4,496 results match your criteria Letters in applied microbiology[Journal]


Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multi-drug resistant motile Aeromonas spp. isolated from ornamental guppy (Poecilia reticulata).

Lett Appl Microbiol 2019 Apr 13. Epub 2019 Apr 13.

Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea.

Aeromonas spp. are opportunistic pathogenic bacteria which are associated with various diseases in ornamental fish, aquaculture raised species, and wild fisheries. In our study, antimicrobial resistance patterns, antimicrobial resistance genes, and class 1 integron gene cassettes of 52 guppy-borne Aeromonas spp. Read More

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http://dx.doi.org/10.1111/lam.13162DOI Listing

Proliferation rate and penicillin binding of enterococcal isolates from patients with immunosupression.

Lett Appl Microbiol 2019 Apr 13. Epub 2019 Apr 13.

Department of Pathology and Experimental Rheumatology, Medical University of Gdańsk, Gdańsk, Poland.

Low proliferation rate of bacterial populations was recently assumed to be a reason for higher resistance to antibiotics and appearance of many chronic infections. Slowly growing populations, called 'small colony variants' (SCVs) have been described in many bacterial species to make from as low as 0,02% up to 46% of population. Thirty enterococcal strains from urine and faeces of renal transplant recipients with asymptomatic, insignificant bacteriuria were studied. Read More

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http://dx.doi.org/10.1111/lam.13161DOI Listing

Clostridium difficile in wild rodents and insectivores in The Netherlands.

Lett Appl Microbiol 2019 Apr 8. Epub 2019 Apr 8.

Institute for Risk Assessment Sciences, Division of Environmental Epidemiology & Veterinary Public Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

With wild rodents and insectivores being present around humans and their living, working, and food production environments, it is important to gain knowledge of the zoonotic pathogens present in these animals. The enteropathogen Clostridium difficile, an opportunistic anaerobic bacteria, can be carried by both animals and humans, and is distributed globally. It is known that there is genetic overlap between human and animal sources of C. Read More

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http://dx.doi.org/10.1111/lam.13159DOI Listing

Comamonas sp. 3ah48 is a dibenz[a,h]anthracene-degrading bacterium that is tolerant to heavy metals.

Lett Appl Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Marine Resources and Energy, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan.

Industrialization often causes polycyclic aromatic hydrocarbon (PAH) and heavy metal contamination of soil and water. In this study, we isolated a bacterium from bottom mud water around a park of Kawasaki Port, Japan, that degrades the 5-ring PAH dibenz[a,h]anthracene (DBA). The strain, Comamonas sp. Read More

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http://dx.doi.org/10.1111/lam.13158DOI Listing
April 2019
2 Reads

Detection of viable but nonculturable Vibrio parahaemolyticus induced by prolonged cold-starvation using propidium monoazide real-time polymerase chain reaction.

Lett Appl Microbiol 2019 Apr 1. Epub 2019 Apr 1.

Department of Food and Nutrition, Chung-Ang University, 4726, Seodong-dearo, Anseong-si, Gyeonggi-do 17546, Republic of Korea.

Viable but nonculturable (VBNC) Vibrio parahaemolyticus cannot be detected by the standard cultivation-based methods. In this study, commonly used viability assessment methods were evaluated for the detection of Vibrio parahaemolyticus in a VBNC state. V. Read More

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http://dx.doi.org/10.1111/lam.13157DOI Listing

Prevalence of Escherichia coli O157 and Salmonella spp. in solid bovine manure in California using real-time quantitative PCR.

Lett Appl Microbiol 2019 Apr 1. Epub 2019 Apr 1.

Deparment of Population Health and Reproduction, School of Veterinary Medicine.

The occurrence of E. coli O157 and Salmonella spp. in solid bovine manure was investigated through a multi-county survey in California. Read More

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http://dx.doi.org/10.1111/lam.13156DOI Listing

Rhenium and Yttrium ions as antimicrobial agents against multidrug resistant Klebsiella pneumonia and Acinetobacter baumanii biofilms.

Lett Appl Microbiol 2019 Mar 31. Epub 2019 Mar 31.

Microbiology at Interfaces Research Group, Manchester Metropolitan University, Chester Street, Manchester, M1 5GD, UK.

Antimicrobial resistance (AMR) presents major global concerns to patient health. In this study, metal ions of Molybdenum, Rhenium, Yttrium and Thallium were tested against bacteria in planktonic and biofilm form using one strain of each of Klebsiella pneumoniae and Acinetobacter baumannii. The antimicrobial efficacy of the metal ions was evaluated against the planktonic bacterial strains using minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs), whilst the efficacy of the metal ions against biofilms was tested using a crystal violet biofilm assay. Read More

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http://dx.doi.org/10.1111/lam.13154DOI Listing
March 2019
1 Read

Diversity of Saccharomyces cerevisiae yeasts associated to spontaneous and inoculated fermenting grapes from Spanish vineyards.

Lett Appl Microbiol 2019 Mar 31. Epub 2019 Mar 31.

Department of Genetics, Physiology and Microbiology. Unit of Microbiology. Biology Faculty, Complutense University of Madrid, 28040, Madrid, Spain.

The use of commercial yeast strains is a common practice in winemaking leading to a predictable quality in wine production, avoiding stuck or sluggish fermentations. However, the use of commercial yeasts leads to a consequent reduction of autochthonous microbial diversity. In this study, one thousand and forty-seven isolates from three Spanish appellations of origin were checked for fingerprinting on interdelta polymorphisms and the strain composition and diversity analyzed using an extensible open-source platform for processing and analysis of an in-house polymorphism database developed for this study. Read More

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http://dx.doi.org/10.1111/lam.13155DOI Listing

Soil attributes in coal mining areas under recovery with bracatinga (Mimosa scabrella).

Lett Appl Microbiol 2019 Mar 28. Epub 2019 Mar 28.

Universidade Federal de Santa Catarina. Campus Trindade. Setor F, Bloco A, Sala 205, Caixa postal 476, CEP: 88040-900, Florianópolis, Santa Catarina, Brasil.

The coal reserves in the south of Brazil were intensely exploited at the time of great demand for such fuel. This resulted in changes in the environment, mainly in the chemical, physical and biological characteristics of the soil. Due to the potential to control erosive processes, increase soil quality and restore biological diversity, revegetation is a promising alternative to recover those impacted areas. Read More

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http://dx.doi.org/10.1111/lam.13153DOI Listing

High occurrence of enterotoxigenic isolates and low antibiotic resistance rates in Staphylococcus aureus isolated from raw milk from cows and ewes.

Lett Appl Microbiol 2019 Mar 28. Epub 2019 Mar 28.

Université de la Manouba, Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Tunisie.

This study aimed to analyse the frequency of genes encoding virulence factors and to characterize resistance profiles in Staphylococcus aureus isolated from raw milk. Forty-seven and nine S. aureus isolates were recovered from 150 and 100 raw bovine and ovine milk samples, respectively, in Tunisia. Read More

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http://dx.doi.org/10.1111/lam.13152DOI Listing

Biofilm in healthcare settings and their control.

Lett Appl Microbiol 2019 Apr;68(4):268

Division of Pharmacy and Optometry, Faculty of Biology, Medicine and Health, Stopford Building, The University of Manchester, Oxford Road, Manchester, M13 9PT.

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http://dx.doi.org/10.1111/lam.13147DOI Listing

Combined effect of conventional antimicrobials with essential oils and their main components against resistant Streptococcus suis strains.

Lett Appl Microbiol 2019 Mar 12. Epub 2019 Mar 12.

Departamento de Sanidad Animal, Universidad de Córdoba, International Excellence Agrifood Campus 'CeiA3', 14071, Córdoba, Spain.

Streptococcus suis is a major swine pathogen. Different strategies to reduce the antimicrobial resistance to conventional antimicrobials (AMBs) have been proposed, including the combined use with essential oils (EOs). The objective of this work was to evaluate the effectiveness of the combination of cinnamon, oregano, common thyme and red thyme EOs and their main components (cinnamaldehyde, carvacrol and thymol) with conventional AMBs (oxytetracycline, trimethoprim-sulfamethoxazole, gentamicin, and penicillin) against field resistant S. Read More

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http://dx.doi.org/10.1111/lam.13151DOI Listing
March 2019
2 Reads

Towards eco-friendly biocides: preparation, antibiofilm activity of hemibastadin analogues.

Lett Appl Microbiol 2019 Apr;68(4):360-368

Université de Toulon, MAPIEM, Toulon, France.

The antibiofilm activity of three hemibastadins analogues was evaluated against different marine bacterial strains through mono-species biofilms and through a multi-species model of biofilm. Results showed that compound 3 exhibited interesting antibiofilm efficiencies effective concentrations (EC ) in the range of 30-100 μmol l without acute toxicity against bacteria. Toxicity against nontargeted organisms was also considered showing that the compound did not affect the global bacterial community at a concentration of 75-100 μmol l . Read More

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http://dx.doi.org/10.1111/lam.13150DOI Listing

Isolation of Desulfovibrio spp. from human gut microbiota using a next-generation sequencing directed culture method.

Lett Appl Microbiol 2019 Mar 5. Epub 2019 Mar 5.

Department of Environmental Health, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, China.

Desulfovibrio spp. is predominant member of sulphate-reducing bacteria in human gut microbiota. Previous studies indicated that the isolation of Desulfovibrio strains from human faecal samples is very important to study the roles of human intestinal Desulfovibrio spp. Read More

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http://dx.doi.org/10.1111/lam.13149DOI Listing

Shochu slop is an excellent medium for Escherichia coli K-12.

Lett Appl Microbiol 2019 Mar 5. Epub 2019 Mar 5.

Education and Research Center for Fermentation Studies, Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, Japan.

We found that shochu slop, the residue generated during the production of distilled shochu liquor, which must be treated as industrial waste, can be used as an excellent medium for Escherichia coli culture. LB medium is generally used in laboratories for culturing E. coli. Read More

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http://dx.doi.org/10.1111/lam.13148DOI Listing

Possible transmission routes of Vibrio spp. in tropical cage-cultured marine fishes.

Lett Appl Microbiol 2019 Mar 4. Epub 2019 Mar 4.

Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, , Selangor, Malaysia.

This study investigates the possible transmission sources of Vibrio spp. in a tropical cage-cultured marine fishes. Samplings of cultured Asian seabass, red snapper, hybrid grouper, wild fish, trash fish, fish fry, water and sediment samples were conducted from December 2016 to August 2017. Read More

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http://dx.doi.org/10.1111/lam.13146DOI Listing

Modulation of Candida albicans virulence in in vitro biofilms by oral bacteria.

Lett Appl Microbiol 2019 Apr;68(4):337-343

School of Dentistry, Cardiff University, Cardiff, UK.

Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of C. albicans virulence factors in in vitro polymicrobial biofilms. Read More

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http://dx.doi.org/10.1111/lam.13145DOI Listing

Bacterial biofilm formation on indwelling urethral catheters.

Lett Appl Microbiol 2019 Apr;68(4):277-293

Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.

Urethral catheters are the most commonly deployed medical devices and used to manage a wide range of conditions in both hospital and community care settings. The use of long-term catheterization, where the catheter remains in place for a period >28 days remains common, and the care of these patients is often undermined by the acquisition of infections and formation of biofilms on catheter surfaces. Particular problems arise from colonization with urease-producing species such as Proteus mirabilis, which form unusual crystalline biofilms that encrust catheter surfaces and block urine flow. Read More

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http://dx.doi.org/10.1111/lam.13144DOI Listing

Artificial dry surface biofilm models for testing the efficacy of cleaning and disinfection.

Lett Appl Microbiol 2019 Apr;68(4):329-336

School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK.

Dry surface biofilms (DSB) harbouring pathogens are widespread in healthcare settings, are difficult to detect and are resistant to cleaning and disinfection interventions. Here, we describe a practical test protocol to palliate the lack of standard efficacy test methods for DSB. Staphylococcus aureus DSB were produced over a 12-day period, grown with or without the presence of organic matter, and their composition and viability were evaluated. Read More

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http://dx.doi.org/10.1111/lam.13143DOI Listing

The health enhancer yeast Saccharomyces cerevisiae in two types of commercial products for animal nutrition.

Lett Appl Microbiol 2019 May 19;68(5):472-478. Epub 2019 Mar 19.

Faculty of Agronomy, Universidad Autónoma de Nuevo León, General Escobedo, México.

The health enhancer yeast Saccharomyces cerevisiae (SC) is widely used in diets for different animals. Two main types of SC-based products are commercially available, one containing live yeasts and one containing SC fermentation by-products, which are supposedly not dependent on live yeasts for their physiological effects in vivo. Culture-based techniques were applied to study yeasts in two types of commercial products: a product containing live SC (LSC) and a SC fermentation product (SCFP). Read More

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http://dx.doi.org/10.1111/lam.13141DOI Listing

Synergistic cranberry juice combinations with natural-borne antimicrobials for the eradication of uropathogenic Escherichia coli biofilm within a short time.

Lett Appl Microbiol 2019 Apr 13;68(4):321-328. Epub 2019 Mar 13.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.

Urinary tract infections (UTI), one of the most common diseases in humans, are caused primarily by uropathogenic Escherichia coli (UPEC). Cranberry juice (CB) is a widely known prophylaxis for UTI, but the treatment of CB alone could not effectively eradicate preformed UPEC biofilms. The aim of this study was to develop enforced CB composites within a short time by adding a small quantity of natural borne antimicrobials. Read More

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http://dx.doi.org/10.1111/lam.13140DOI Listing
April 2019
1 Read

Research and molecular characteristic of Shiga toxin-producing Escherichia coli isolated from sheep carcasses.

Lett Appl Microbiol 2019 Feb 23. Epub 2019 Feb 23.

Laboratory of Food Hygiene and Quality Insurance System, High National Veterinary School of Algiers, Algiers, Algeria.

Domestic ruminants are regarded as the major reservoir of Shiga toxin-producing Escherichia coli (STEC) closely related to human infection. A total of 363 ovine carcasses were swabbed in an Algiers city slaughterhouse for research on STEC. First of all, screening of the STECs was carried out by a multiplex PCR searching for the genes coding for the virulence factors stx , stx and eae. Read More

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http://dx.doi.org/10.1111/lam.13142DOI Listing
February 2019
3 Reads

A novel quantitative polymerase chain reaction to monitor urinary tract mycoplasma infection in a dog.

Lett Appl Microbiol 2019 May 19;68(5):409-414. Epub 2019 Mar 19.

School of Animal and Veterinary Science, The University of Adelaide, Adelaide, SA, Australia.

The aim of the study was to develop a quantitative real-time PCR assay for diagnosis and monitoring of mycoplasma urinary tract infections (UTI) in a dog. An English Cocker Spaniel dog with the history of urinary tract infection was physically examined and laboratory findings identified chronic renal insufficiency and urinary tract infection. Attempts to culture organisms from pyuric urine failed, and empirical antibiotic therapy did not resolve the pyuria. Read More

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http://dx.doi.org/10.1111/lam.13139DOI Listing
May 2019
1 Read
1.659 Impact Factor

Towards application of water extract from heat-killed Lactococcus lactis H61 as a cosmetic ingredient.

Lett Appl Microbiol 2019 Feb 21. Epub 2019 Feb 21.

NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.

We previously reported that oral administration of heat-killed Lactococcus lactis H61 improves certain human skin properties. For topical application of this strain, we reasoned that a bacterial cell extract obtained with an aqueous solvent could be readily formulated as a cosmetic ingredient. In the present study, we characterized the water extract from heat-killed H61. Read More

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http://dx.doi.org/10.1111/lam.13136DOI Listing
February 2019

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes.

Lett Appl Microbiol 2019 May 19;68(5):370-377. Epub 2019 Mar 19.

Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.

Aeromonas spp. are Gram-negative opportunistic bacteria which have been commonly associated with fish diseases. In this study, antibiogram, antimicrobial resistance genes and integrons of 43 zebrafish-borne Aeromonas spp. Read More

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http://dx.doi.org/10.1111/lam.13138DOI Listing

The use of selected phytochemicals with EDTA against Escherichia coli and Staphylococcus epidermidis single- and dual-species biofilms.

Lett Appl Microbiol 2019 Apr 13;68(4):313-320. Epub 2019 Mar 13.

LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.

The aim of this study was the development of a novel and effective antibacterial formulation combining selected phytochemical compounds (quercetin, cuminaldehyde, indole-3-carbinol and vanillic acid) with ethylenediaminetetraacetic acid (EDTA), an aminopolycarboxylic acid. The antibacterial activity of the combinations was evaluated against Escherichia coli and Staphylococcus epidermidis in planktonic and sessile states as single and dual species. The compounds when applied individually demonstrated modest antibacterial activity. Read More

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http://doi.wiley.com/10.1111/lam.13137
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http://dx.doi.org/10.1111/lam.13137DOI Listing
April 2019
1 Read
1.659 Impact Factor

Detection of Bacillus anthracis in animal tissues using InBios active anthrax detect rapid test lateral flow immunoassay.

Lett Appl Microbiol 2019 Feb 18. Epub 2019 Feb 18.

Centers for Disease Control and Prevention, Atlanta, GA, USA.

The Active Anthrax Detect (AAD) Rapid Test lateral flow immunoassay is a point-of-care assay that was under investigational use for detecting Bacillus anthracis capsular polypeptide (polyglutamic acid) in human blood, serum and plasma. Small sample volumes, rapid results and no refrigeration required allow for easy use in either the field or laboratory. Although the test was developed for use in suspect cases of human inhalation anthrax, its features also make it a potentially powerful tool for testing suspect animal cases. Read More

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http://doi.wiley.com/10.1111/lam.13134
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http://dx.doi.org/10.1111/lam.13134DOI Listing
February 2019
11 Reads

Alteration of crop rotation in continuous Pinellia ternate cropping soils profiled via fungal ITS amplicon sequencing.

Lett Appl Microbiol 2019 Feb 18. Epub 2019 Feb 18.

College of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, 311300, China.

Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Read More

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http://dx.doi.org/10.1111/lam.13135DOI Listing
February 2019
1.659 Impact Factor

Antagonistic effects of Streptococcus and Lactobacillus probiotics in pharyngeal biofilms.

Lett Appl Microbiol 2019 Apr 13;68(4):303-312. Epub 2019 Mar 13.

Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.

Direct antagonism towards pathogens including Streptococcus pyogenes is a proposed mechanism of pharyngeal probiosis but off-target effects on the symbiotic microbiota of the throat are possible and may be beneficial, harmful or neutral. We have assessed the bacteriological effects of two candidate Lactobacillus probiotics and the established pharyngeal probiotic Streptococcus salivarius K12. Antagonism towards S. Read More

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http://dx.doi.org/10.1111/lam.13133DOI Listing

Impact of plant growth promoting rhizobacteria on different forms of soil potassium under wheat cultivation.

Lett Appl Microbiol 2019 Feb 17. Epub 2019 Feb 17.

Soil Science Department, Agriculture Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

This study aimed to investigate the effect of some plant growth promoting rhizobacteria with potassium dissolution ability on different forms of potassium in soil under the cultivation of wheat. The factorial experiment was conducted as a randomized complete design with three replications in greenhouse conditions. The treatments consisted of bacterium inoculation (without inoculation, Enterobacter cloacae Rhizo_33, Pseudomonas sp. Read More

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http://dx.doi.org/10.1111/lam.13132DOI Listing
February 2019
1 Read

An in vitro biofilm model of Staphylococcus aureus infection of bone.

Lett Appl Microbiol 2019 Apr 13;68(4):294-302. Epub 2019 Mar 13.

Department of Applied Sciences, University of the West of England, Bristol, UK.

Chronic osteomyelitis is difficult to treat, with biofilm growth and the diffusion barrier to antibiotics presented by bone contributory factors. The aim of this study was to develop and evaluate an in vitro model of osteomyelitis. A bioluminescent strain of Staphylococcus aureus was grown in bone blocks made from bovine femur. Read More

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http://dx.doi.org/10.1111/lam.13131DOI Listing

Anti-biofilm and cytoprotective activities of quercetin against Pseudomonas aeruginosa isolates.

Lett Appl Microbiol 2019 May 19;68(5):464-471. Epub 2019 Mar 19.

Yenepoya Research Centre, Yenepoya University, Mangalore, India.

Increase in infection with multidrug resistant Pseudomonas aeruginosa is a serious global challenge in healthcare. Pseudomonas aeruginosa is capable of causing human infection in various sites and complicates the infection due to its virulence factors. This study was aimed to investigate the effect of quercetin, a dietary flavonoid against the virulence factors of P. Read More

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http://dx.doi.org/10.1111/lam.13129DOI Listing
May 2019
1 Read

Survey on the CRISPR arrays in Lactobacillus helveticus genomes.

Lett Appl Microbiol 2019 May 21;68(5):394-402. Epub 2019 Mar 21.

CREA Research Centre for Animal Production and Aquaculture (CREA-ZA), Lodi, Italy.

Lactobacillus helveticus is a homofermentative thermophilic lactic acid bacteria that is mainly used in the manufacture of Swiss type and long-ripened Italian hard cheeses. In this study, the presence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were analysed in 25 L. helveticus genomes and identified in 23 of these genomes. Read More

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http://doi.wiley.com/10.1111/lam.13128
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http://dx.doi.org/10.1111/lam.13128DOI Listing
May 2019
12 Reads

Loop-mediated isothermal amplification (LAMP) for paratyphoid fever - a proof-of-principle analysis.

Lett Appl Microbiol 2019 Feb 14. Epub 2019 Feb 14.

Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany.

In-house loop-mediated isothermal amplification (LAMP) procedures for the detection of paratyphoid fever associated bacteria on serovar level were evaluated. Therefore, LAMP primers for Salmonella genus, for two LAMP schemes for S. Paratyphi A, for S. Read More

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http://doi.wiley.com/10.1111/lam.13130
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http://dx.doi.org/10.1111/lam.13130DOI Listing
February 2019
7 Reads

Biofilm contamination of high-touched surfaces in intensive care units: epidemiology and potential impacts.

Lett Appl Microbiol 2019 Apr 15;68(4):269-276. Epub 2019 Mar 15.

Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia.

The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susceptibility profiles) of the bacterial contamination on intensive care units (ICUs) surfaces. Fifty-seven high-touched surfaces were collected from adult, paediatric and neonatal ICUs from two large public Brazilian hospitals from central and north regions. Samples (c. Read More

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http://dx.doi.org/10.1111/lam.13127DOI Listing

Pharmacodynamics of ciprofloxacin against Pseudomonas aeruginosa planktonic and biofilm-derived cells.

Lett Appl Microbiol 2019 Apr 7;68(4):350-359. Epub 2019 Mar 7.

Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, UK.

The influence of growth phase and state on the survival and recovery of Pseudomonas aeruginosa exposed to ciprofloxacin was investigated using batch culture grown planktonic cells and disaggregated biofilm populations. Biofilms were either nonantibiotic exposed or previously exposed to ciprofloxacin before disaggregation and subsequent challenge with ciprofloxacin. Viable counts showed that late stationary phase cells were tolerant to ciprofloxacin over 24 h exposure, while all other populations presented a biphasic killing pattern. Read More

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http://doi.wiley.com/10.1111/lam.13126
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http://dx.doi.org/10.1111/lam.13126DOI Listing
April 2019
7 Reads

Biological ball filters regulate bacterial communities in marron (Cherax cainii) culture system.

Lett Appl Microbiol 2019 May 19;68(5):455-463. Epub 2019 Mar 19.

School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.

This study aimed to characterize the bacterial communities in rearing water treated with commercial plastic biological ball filters named as Bio-ball in marron culture for 60 days. Inclusion of Bio-ball in the aquaculture tanks showed improvement in water quality parameters and enrichment of bacterial communities in terms of operational taxonomic units. The water treated with Bio-ball showed significantly less nitrate, nitrite, ammonia, phosphorus and high dissolved oxygen concentration than untreated control group. Read More

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http://dx.doi.org/10.1111/lam.13125DOI Listing

Suppressive effect of Lactobacillus fermentum Lim2 on Clostridioides difficile 027 toxin production.

Lett Appl Microbiol 2019 May 24;68(5):386-393. Epub 2019 Mar 24.

Division of Animal Science, Chonnam National University, Gwangju, Korea.

Clostridioides difficile is a spore-forming, Gram-positive, anaerobic pathogen that caused gastrointestinal illness. During dysbiosis, overgrowth of C. difficile resulting in higher levels of toxin production. Read More

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http://dx.doi.org/10.1111/lam.13124DOI Listing
May 2019
2 Reads

Acinetobacter baumannii biofilm biomass mediates tolerance to cold plasma.

Lett Appl Microbiol 2019 Apr 13;68(4):344-349. Epub 2019 Mar 13.

Biofilm Research Group, School of Pharmacy, Queen's University, Belfast, UK.

Acinetobacter baumannii is an intrinsically multidrug-resistant pathogen that, when existing as a biofilm, confers increased environmental tolerance to desiccation, nutrient starvation as well as increased tolerance to antimicrobials. Outbreaks of A. baumannii infections within the clinical setting are often associated with the biofilm phenotype. Read More

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http://dx.doi.org/10.1111/lam.13122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446819PMC

Highly selective resolution of racemic 1-phenyl-1,2-ethanediol by a novel strain Kurthia gibsonii SC0312.

Lett Appl Microbiol 2019 May 19;68(5):446-454. Epub 2019 Mar 19.

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

Chiral 1-phenyl-1,2-ethanediol (PED) performs vital effect for the preparation of pharmaceuticals, agrochemicals and cosmetics. In the study, a newly isolated strain Kurthia gibsoniiSC0312 with the ability to selectively oxidize racemic PED to achieve (S)-PED was evaluated in the aqueous reaction system. The strain showed excellent catalytic performances within the range of pH 5·5-8·5, temperature 25-45°C and the amount of cell 15 mg ml to 30 mg ml . Read More

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http://dx.doi.org/10.1111/lam.13123DOI Listing

Highly variable vancomycin-resistant enterococci in the north-eastern part of the Czech Republic.

Lett Appl Microbiol 2019 Jan 29. Epub 2019 Jan 29.

Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.

Vancomycin-resistant enterococci (VRE) are nosocomial pathogens of increasing medical importance. This study involved 121 VRE selectively obtained from a representative set of 1464 samples collected from various sources in the north-eastern part of the Czech Republic. In total, 119 VRE belonged to Enterococcus faecium and two to Enterococcus faecalis. Read More

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http://dx.doi.org/10.1111/lam.13121DOI Listing
January 2019

Aporphinoid alkaloids as antimicrobial agents against Yersinia enterocolitica.

Lett Appl Microbiol 2019 May 8;68(5):437-445. Epub 2019 Mar 8.

Instituto de Investigación en Tecnología Química- Consejo Nacional de Investigaciones Científicas y Técnicas (INTEQUI-CONICET), San Luis, Argentina.

Foodborne diseases have become a health issue worldwide, mainly due to the consumption of contaminated foods that are either raw, improperly heat treated or cross-contaminated after adequate heat treatment foods. A group of alkaloids extracted from plants were tested to evaluate their antimicrobial effect against different strains of Yersinia enterocolitica and other foodborne bacteria. The results obtained reveal that oliveridine and pachypodanthine inhibited Y. Read More

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http://dx.doi.org/10.1111/lam.13120DOI Listing
May 2019
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Characterization of the bacterial microbiome in first-pass meconium using propidium monoazide (PMA) to exclude nonviable bacterial DNA.

Lett Appl Microbiol 2019 May 15;68(5):378-385. Epub 2019 Feb 15.

Division of Obstetrics and Gynaecology, Faculty of Health & Medical Sciences, The University of Western Australia, Crawley, WA, Australia.

Numerous studies have reported bacterial DNA in first-pass meconium samples, suggesting that the human gut microbiome is seeded prior to birth. However, these studies have not been able to discriminate between DNA from living bacterial cells, DNA from dead bacterial cells or cell-free DNA. Here we have used propidium monoazide (PMA) together with 16S rRNA gene sequencing to determine whether there are intact bacterial cells in the fetal gut. Read More

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Plasmid curing resulted in improved heterologous gene expression in Leuconostoc citreum EFEL2700.

Lett Appl Microbiol 2019 May 20;68(5):430-436. Epub 2019 Feb 20.

Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Korea.

Leuconostoc citreum EFEL2700 isolated from kimchi was used as a host strain for genetic and metabolic engineering in our previous studies, but the cells of EFEL2700 contained a cryptic plasmid (P-cells). Thus, we created plasmid-free cells (F-cells) using the CRISPR/Cas9 system. In this study, we compared the microbial characteristics of P- and F-cells in terms of growth rate, biochemical properties, transformation efficiency, plasmid copy number and protein expression level. Read More

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http://doi.wiley.com/10.1111/lam.13118
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http://dx.doi.org/10.1111/lam.13118DOI Listing
May 2019
9 Reads
1.659 Impact Factor

Plant growth promotion and suppression of bacterial leaf blight in rice by Paenibacillus polymyxa Sx3.

Lett Appl Microbiol 2019 May 18;68(5):423-429. Epub 2019 Feb 18.

State Key laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

The effects and mechanisms of Paenibacillus polymyxa Sx3 on growth promotion and the suppression of bacterial leaf blight in rice were evaluated in this study. The results from a plate assay indicated that Sx3 inhibited the growth of 20 strains of Xanthomonas oryzae pv. oryzae (Xoo). Read More

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http://dx.doi.org/10.1111/lam.13117DOI Listing
May 2019
11 Reads

Empowering rice seedling growth by endophytic Bradyrhizobium sp. SUTN9-2.

Lett Appl Microbiol 2019 Mar 6;68(3):258-266. Epub 2019 Feb 6.

School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

Bradyrhizobium sp. strain SUTN9-2 was confirmed as rice endophytic bacteria and also as rice growth promotion agent. SUTN9-2 showed the capability of plant growth promotion characteristics, such as indole-3-acetic acid (IAA) and 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase productions and nitrogen fixation. Read More

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http://doi.wiley.com/10.1111/lam.13114
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http://dx.doi.org/10.1111/lam.13114DOI Listing
March 2019
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Detection of Talaromyces macrosporus and Talaromyces trachyspermus by a PCR assay targeting the hydrophobin gene.

Lett Appl Microbiol 2019 May 31;68(5):415-422. Epub 2019 Jan 31.

R & D Center, Aohata Corporation, Takehara, Hiroshima, Japan.

Talaromyces species are typical fungi capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Hydrophobins, which are unique to fungi, are small secreted proteins that form amphipathic layers on the outer surface of fungal cell walls. In this study, species-specific primer sets for detecting and identifying Talaromyces macrosporus and Talaromyces trachyspermus were designed based on hydrophobin gene sequences. Read More

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http://doi.wiley.com/10.1111/lam.13116
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May 2019
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A loop-mediated isothermal amplification assay for the diagnosis of pulmonary tuberculosis.

Lett Appl Microbiol 2019 Mar;68(3):219-225

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Quantitated Mycobacterium tuberculosis (M.tb) H37Rv DNA was used to analyse the sensitivity and the specificity was assessed using DNA isolated from the reference strain H37Rv, 12 nontuberculous mycobacterium (NTM) species and five nonmycobacterium species. Furthermore, performance of the assay was evaluated on the sputum samples and compared with smear microscopy, culture and PCR. Read More

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http://dx.doi.org/10.1111/lam.13115DOI Listing
March 2019
5 Reads
1.659 Impact Factor

Putative virulence factors of extra-intestinal Escherichia coli isolated from bovine mastitis with different clinical scores.

Lett Appl Microbiol 2019 May 8;68(5):403-408. Epub 2019 Mar 8.

Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, Botucatu, Brazil.

We investigated the genes kpsMTII, iucD, sfaDE, afaBC, papA and papC, (proposed to be involved in extra-intestinal pathogenic Escherichia coli-ExPEC), phylogroup classification and the in vitro swimming and swarming motility in 50 E. coli isolated from bovine mastitis with different clinical severity scores (mild, moderate and severe). The aforementioned genes were detected in 12 (n = 12/50; 24·0%) isolates. Read More

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http://dx.doi.org/10.1111/lam.13113DOI Listing

Three species of Aeromonas (A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia.

Lett Appl Microbiol 2019 Mar 11;68(3):212-218. Epub 2019 Feb 11.

Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.

Hundreds of farmed Siamese crocodiles (Crocodylus siamensis) died during July 2016 at a farm in Wenchang, Hainan, China. In two necropsied crocodiles, we observed symptoms of dermatorrhagia, hepatomegaly and hepatic congestion. Pulmonitis was diagnosed by pulmonary congestion and pulmonary fibrinous exudate. Read More

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http://doi.wiley.com/10.1111/lam.13112
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http://dx.doi.org/10.1111/lam.13112DOI Listing
March 2019
23 Reads
1.659 Impact Factor