4,474 results match your criteria Letters in applied microbiology[Journal]


A novel quantitative polymerase chain reaction to monitor urinary tract mycoplasma infection in a dog.

Lett Appl Microbiol 2019 Feb 21. Epub 2019 Feb 21.

School of Animal and Veterinary Science, The University of Adelaide, Adelaide, Australia.

The aim of the study was to develop a qPCR assay for diagnosis and monitoring of mycoplasma urinary tract infections (UTI) in a dogs. An English Cocker Spaniel dog with the history of urinary tract infection was physically examined and laboratory findings identified chronic renal insufficiency and urinary tract infection. Attempts to culture organisms from pyuric urine failed, and empirical antibiotic therapy did not resolve the pyuria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13139DOI Listing
February 2019

Towards application of water extract from heat-killed Lactococcus lactis H61 as a cosmetic ingredient.

Lett Appl Microbiol 2019 Feb 21. Epub 2019 Feb 21.

NARO Institute of Livestock and Grassland Science, Ikenodai 2, Tsukuba, Ibaraki, 305-0901, Japan.

We previously reported that oral administration of heat-killed Lactococcus lactis H61 improves certain human skin properties. For topical application of this strain, we reasoned that a bacterial cell extract obtained with an aqueous solvent could be readily formulated as a cosmetic ingredient. In the present study, we characterized the water extract from heat-killed H61. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13136DOI Listing
February 2019

Multi-drug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): Antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes.

Lett Appl Microbiol 2019 Feb 20. Epub 2019 Feb 20.

Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea.

Aeromonas spp. are Gram-negative opportunistic bacteria which have been commonly associated with fish diseases. In this study, antibiogram, antimicrobial resistance genes and integrons of 43 zebrafish-borne Aeromonas spp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13138DOI Listing
February 2019

The use of selected phytochemicals with EDTA against Escherichia coli and Staphylococcus epidermidis single and dual-species biofilms.

Lett Appl Microbiol 2019 Feb 21. Epub 2019 Feb 21.

LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465, Porto, Portugal.

The aim of this study was the development of a novel and effective antibacterial formulation combining selected phytochemical compounds (quercetin, cuminaldehyde, indole-3-carbinol and vanillic acid) with ethylenediaminetetraacetic acid (EDTA), an aminopolycarboxylic acid. The antibacterial activity of the combinations was evaluated against Escherichia coli and Staphylococcus epidermidis in planktonic and sessile states as single and dual-species. The compounds when applied individually demonstrated modest antibacterial activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13137DOI Listing
February 2019

Detection of Bacillus anthracis in Animal Tissues using InBios Active Anthrax Detect Rapid Test Lateral Flow Immunoassay.

Lett Appl Microbiol 2019 Feb 18. Epub 2019 Feb 18.

Centers for Disease Control and Prevention, Atlanta, Georgia, 30333.

The Active Anthrax Detect (AAD) Rapid Test lateral flow immunoassay is a point-of-care assay that was under investigational use for detecting Bacillus anthracis capsular polypeptide (polyglutamic acid) in human blood, serum, and plasma. Small sample volumes, rapid results, and no refrigeration required allow for easy use in either the field or laboratory. Although the test was developed for use in suspect cases of human inhalation anthrax, its features also make it a potentially powerful tool for testing suspect animal cases. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13134
Publisher Site
http://dx.doi.org/10.1111/lam.13134DOI Listing
February 2019
2 Reads

Alteration of Crop Rotation in Continuous Pinellia ternate Cropping Soils Profiled via ITS Amplicon Sequencing.

Lett Appl Microbiol 2019 Feb 18. Epub 2019 Feb 18.

Moutai College, Department of Liquor Making Engineering, Renhuai, 564500, China.

Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13135DOI Listing
February 2019

Antagonistic effects of Streptococcus and Lactobacillus probiotics in pharyngeal biofilms.

Lett Appl Microbiol 2019 Feb 18. Epub 2019 Feb 18.

Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Stopford Building, M13 9PT, UK.

Direct antagonism towards pathogens including Streptococcus pyogenes is a proposed mechanism of pharyngeal probiosis but off-target effects on the symbiotic microbiota of the throat are possible and may be beneficial, harmful or neutral. We have assessed the bacteriological effects of two candidate Lactobacillus probiotics and the established pharyngeal probiotic S. salivarius K12. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13133DOI Listing
February 2019

Impact of Plant Growth Promoting Rhizobacteria on Different Forms of Soil Potassium under Wheat Cultivation.

Lett Appl Microbiol 2019 Feb 17. Epub 2019 Feb 17.

Soil Science Department, Agriculture Faculty, Shahid Chamran University of Ahvaz, Iran.

This study aimed to investigate the effect of some plant growth promoting rhizobacteria with potassium dissolution ability on different forms of potassium in soil under the cultivation of wheat. The factorial experiment was conducted as a randomized complete design with three replications in greenhouse conditions. The treatments consisted of bacterium inoculation (without inoculation, Enterobacter cloacae Rhizo_33, Pseudomonas sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13132DOI Listing
February 2019

An in vitro biofilm model of Staphylococcus aureus infection of bone.

Lett Appl Microbiol 2019 Feb 15. Epub 2019 Feb 15.

Department of Applied Sciences, University of the West of England, Bristol, Frenchay Campus, Coldharbour Lane, Bristol, BS16 1QY.

Chronic osteomyelitis is difficult to treat, with biofilm growth and the diffusion barrier to antibiotics presented by bone contributory factors. The aim of this study was to develop and evaluate an in vitro model of osteomyelitis. A bioluminescent strain of Staphylococcus aureus was grown in bone blocks made from bovine femur. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13131DOI Listing
February 2019

Anti-biofilm and cytoprotective activities of quercetin against Pseudomonas aeruginosa isolates.

Lett Appl Microbiol 2019 Feb 14. Epub 2019 Feb 14.

Yenepoya Research Centre, Yenepoya University, Mangalore, 575018, Karnataka, India.

Increase in infection with multidrug resistant Pseudomonas aeruginosa is a serious global challenge in healthcare. P. aeruginosa is capable of causing human infection in various sites and complicates the infection due to its virulence factors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13129DOI Listing
February 2019

Survey on the CRISPR arrays in Lactobacillus helveticus genomes.

Lett Appl Microbiol 2019 Feb 14. Epub 2019 Feb 14.

CREA Research Centre for Animal Production and Aquaculture (CREA-ZA), Lodi, Italy.

Lactobacillus helveticus is a homofermentative thermophilic LAB that is mainly used in the manufacture of Swiss type and long-ripened Italian hard cheeses. In this study, the presence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were analysed in 25 L. helveticus genomes and identified in 23 of these genomes. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13128
Publisher Site
http://dx.doi.org/10.1111/lam.13128DOI Listing
February 2019
4 Reads

Loop-mediated isothermal amplification (LAMP) for paratyphoid fever - a proof-of-principle analysis.

Lett Appl Microbiol 2019 Feb 14. Epub 2019 Feb 14.

Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany.

In-house loop-mediated isothermal amplification (LAMP) procedures for the detection of paratyphoid fever associated bacteria on serovar level were evaluated. Therefore, LAMP primers for Salmonella genus, for two LAMP schemes for S. Paratyphi A, for S. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13130
Publisher Site
http://dx.doi.org/10.1111/lam.13130DOI Listing
February 2019
3 Reads

Biofilm contamination of high-touched surfaces in intensive care units: epidemiology and potential impacts.

Lett Appl Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.

The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm, and pathogens, including ESKAPE - Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susceptibility profiles) of the bacterial contamination on intensive care units (ICUs) surfaces. Fifty-seven high-touched surfaces were collected from adult, paediatric and neonatal ICUs from two large public Brazilian hospitals from central and north regions. Samples (~4cm ) were subjected to culture (qualitative), qPCR targeting 16s rRNA gene (microbial load - bacteria/cm ), 16s rRNA amplicon sequencing (microbiome analysis) and scanning electron (SEM) or confocal laser scanning microscopy (CLSM) (biofilm presence). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13127DOI Listing
February 2019

Pharmacodynamics of ciprofloxacin against Pseudomonas aeruginosa planktonic and biofilm-derived cells.

Lett Appl Microbiol 2019 Feb 11. Epub 2019 Feb 11.

Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, Coldharbour Lane, Bristol, BS16 1QY, England, United Kingdom.

The influence of growth phase and state on survival and recovery of Pseudomonas aeruginosa exposed to ciprofloxacin was investigated using batch culture grown planktonic cells and disaggregated biofilm populations. Biofilms were either non-antibiotic exposed or previously exposed to ciprofloxacin before disaggregation and subsequent challenge with ciprofloxacin. Viable counts showed that late stationary phase cells were tolerant to ciprofloxacin over 24 h exposure, whilst all other populations presented a biphasic killing pattern. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13126
Publisher Site
http://dx.doi.org/10.1111/lam.13126DOI Listing
February 2019
1 Read

Biological ball filters regulate bacterial communities in marron (Cherax cainii) culture system.

Lett Appl Microbiol 2019 Feb 4. Epub 2019 Feb 4.

School of Molecular and Life Sciences, Curtin University, Bentley, WA, 6845, Australia.

This study aimed to characterize the bacterial communities in rearing water treated with commercial plastic biological ball filters named as Bio-ball in marron culture for 60 days. Inclusion of Bio-ball in the aquaculture tanks showed improvement in water quality parameters and enrichment of bacterial communities in terms of operational taxonomic units (OTUs). The water treated with Bio-ball showed significantly less nitrate, nitrite, ammonia, phosphorus and high dissolved oxygen concentration than untreated control group. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13125DOI Listing
February 2019

Suppressive effect of Lactobacillus fermentum Lim2 on Clostridioides difficile 027 toxin production.

Lett Appl Microbiol 2019 Feb 3. Epub 2019 Feb 3.

Division of Animal Science, Chonnam National University, Gwangju, 61186, Korea.

Clostridioides difficile is a spore-forming, Gram-positive, anaerobic pathogen that caused gastrointestinal illness. During dysbiosis, overgrowth of C. difficile resulting in higher levels of toxin production. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13124DOI Listing
February 2019
1 Read

Acinetobacter baumannii Biofilm Biomass Mediates Tolerance to Cold Plasma Exposure.

Lett Appl Microbiol 2019 Feb 1. Epub 2019 Feb 1.

Biofilm Research Group, School of Pharmacy, Queen's University Belfast, BT9 7BL, UK.

Acinetobacter baumannii is an intrinsically multi-drug resistant pathogen that, when existing as a biofilm, confers increased environmental tolerance to desiccation, nutrient starvation as well as increased tolerance to antimicrobials. Outbreaks of A. baumannii infections within the clinical setting are often associated with the biofilm phenotype. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13122DOI Listing
February 2019

Highly selective resolution of racemic 1-phenyl-1,2-ethanediol by a novel strain Kurthia gibsonii SC0312.

Lett Appl Microbiol 2019 Jan 31. Epub 2019 Jan 31.

Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.

Chiral 1-phenyl-1,2-ethanediol (PED) performs vital effect for the preparation of pharmaceuticals, agrochemicals, and cosmetics. In the study, a newly isolated strain Kurthia gibsonii SC0312 with the ability to selectively oxidize racemic PED to achieve (S)-PED was evaluated in the aqueous reaction system. The strain showed excellent catalytic performances within the range of pH 5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13123DOI Listing
January 2019

Highly variable vancomycin-resistant enterococci in the north-eastern part of the Czech Republic.

Lett Appl Microbiol 2019 Jan 29. Epub 2019 Jan 29.

Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.

Vancomycin-resistant enterococci (VRE) are nosocomial pathogens of increasing medical importance. This study involved 121 VRE selectively obtained from a representative set of 1464 samples collected from various sources in the north-eastern part of the Czech Republic. In total, 119 VRE belonged to Enterococcus faecium and two to Enterococcus faecalis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13121DOI Listing
January 2019

Aporphinoid alkaloids as antimicrobial agents against Yersinia enterocolitica.

Lett Appl Microbiol 2019 Jan 27. Epub 2019 Jan 27.

Instituto de Investigación en Tecnología Química- Consejo Nacional de Investigaciones Científicas y Técnicas (INTEQUI- CONICET), Almirante Brown 1455, San Luis, P.O. 5700, Argentina.

Foodborne diseases have become a health issue worldwide, mainly due to the consumption of contaminated foods that are either raw, improperly heat treated or crosscontaminated after adequate heat treatment foods. A group of alkaloids extracted from plants were tested to evaluate their antimicrobial effect against different strains of Yersinia enterocolitica and other foodborne bacteria. The results obtained reveal that oliveridine and pachypodanthine inhibited Y. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13120DOI Listing
January 2019

Characterization of the bacterial microbiome in first-pass meconium using propidium monoazide (PMA) to exclude nonviable bacterial DNA.

Lett Appl Microbiol 2019 Jan 23. Epub 2019 Jan 23.

Division of Obstetrics and Gynaecology, Faculty of Health & Medical Sciences, The University of Western Australia, Crawley, WA, Australia.

Numerous studies have reported bacterial DNA in first-pass meconium samples, suggesting that the human gut microbiome is seeded prior to birth. However, these studies have not been able to discriminate between DNA from living bacterial cells, DNA from dead bacterial cells or cell-free DNA. Here we have used propidium monoazide (PMA) together with 16S rRNA gene sequencing to determine whether there are intact bacterial cells in the fetal gut. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13119DOI Listing
January 2019

Plasmid curing resulted in improved heterologous gene expression in Leuconostoc citreum EFEL2700.

Lett Appl Microbiol 2019 Jan 21. Epub 2019 Jan 21.

Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Korea.

Leuconostoc citreum EFEL2700 isolated from kimchi was used as a host strain for genetic and metabolic engineering in our previous studies, but the cells of EFEL2700 contained a cryptic plasmid (P-cells). Thus, we created plasmid-free cells (F-cells) using the CRISPR/Cas9 system. In this study, we compared the microbial characteristics of P- and F-cells in terms of growth rate, biochemical properties, transformation efficiency, plasmid copy number and protein expression level. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13118
Publisher Site
http://dx.doi.org/10.1111/lam.13118DOI Listing
January 2019
6 Reads
1.659 Impact Factor

Plant growth promotion and suppression of bacterial leaf blight in rice by Paenibacillus polymyxa Sx3.

Lett Appl Microbiol 2019 Jan 19. Epub 2019 Jan 19.

State Key laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

The effects and mechanisms of Paenibacillus polymyxa Sx3 on growth promotion and the suppression of bacterial leaf blight in rice were evaluated in this study. The results from a plate assay indicated that Sx3 inhibited the growth of 20 strains of Xanthomonas oryzae pv. oryzae (Xoo). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13117DOI Listing
January 2019
3 Reads

Empowering rice seedling growth by endophytic Bradyrhizobium sp. SUTN9-2.

Lett Appl Microbiol 2019 Mar 6;68(3):258-266. Epub 2019 Feb 6.

School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

Bradyrhizobium sp. strain SUTN9-2 was confirmed as rice endophytic bacteria and also as rice growth promotion agent. SUTN9-2 showed the capability of plant growth promotion characteristics, such as indole-3-acetic acid (IAA) and 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase productions and nitrogen fixation. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13114
Publisher Site
http://dx.doi.org/10.1111/lam.13114DOI Listing
March 2019
10 Reads

Detection of Talaromyces macrosporus and Talaromyces trachyspermus by a PCR assay targeting the hydrophobin gene.

Lett Appl Microbiol 2019 Jan 12. Epub 2019 Jan 12.

R & D Center, Aohata Corporation, Takehara, Hiroshima, Japan.

Talaromyces species are typical fungi capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Hydrophobins, which are unique to fungi, are small secreted proteins that form amphipathic layers on the outer surface of fungal cell walls. In this study, species-specific primer sets for detecting and identifying Talaromyces macrosporus and Talaromyces trachyspermus were designed based on hydrophobin gene sequences. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13116
Publisher Site
http://dx.doi.org/10.1111/lam.13116DOI Listing
January 2019
1 Read

A loop-mediated isothermal amplification assay for the diagnosis of pulmonary tuberculosis.

Lett Appl Microbiol 2019 Mar;68(3):219-225

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Quantitated Mycobacterium tuberculosis (M.tb) H37Rv DNA was used to analyse the sensitivity and the specificity was assessed using DNA isolated from the reference strain H37Rv, 12 nontuberculous mycobacterium (NTM) species and five nonmycobacterium species. Furthermore, performance of the assay was evaluated on the sputum samples and compared with smear microscopy, culture and PCR. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13115DOI Listing
March 2019
5 Reads
1.659 Impact Factor

Putative virulence factors of extra-intestinal Escherichia coli isolated from bovine mastitis with different clinical scores.

Lett Appl Microbiol 2019 Jan 10. Epub 2019 Jan 10.

Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, São Paulo State University - UNESP, Botucatu, SP, Brazil.

We investigated the genes kpsMTII, iucD, sfaDE, afaBC, papA, and papC, (proposed to be involved in extra-intestinal pathogenic E. coli - ExPEC), phylogroup classification, and the in vitro swimming and swarming motility in 50 E. coli isolated from bovine mastitis with different clinical severity scores (mild, moderate and severe). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13113DOI Listing
January 2019

Three species of Aeromonas (A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia.

Lett Appl Microbiol 2019 Mar 11;68(3):212-218. Epub 2019 Feb 11.

Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.

Hundreds of farmed Siamese crocodiles (Crocodylus siamensis) died during July 2016 at a farm in Wenchang, Hainan, China. In two necropsied crocodiles, we observed symptoms of dermatorrhagia, hepatomegaly and hepatic congestion. Pulmonitis was diagnosed by pulmonary congestion and pulmonary fibrinous exudate. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13112
Publisher Site
http://dx.doi.org/10.1111/lam.13112DOI Listing
March 2019
6 Reads
1.659 Impact Factor

Intracellular expression of Vitreoscilla haemoglobin improves lipid production in Yarrowia lipolytica.

Lett Appl Microbiol 2019 Mar 17;68(3):248-257. Epub 2019 Jan 17.

Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.

Genetic modification by overexpressing Vitreoscilla haemoglobin (VHb) is an efficient and economic method for improving O utilization for microbial fermentation. In this study, VHb was expressed in oleaginous yeast, Yarrowia lipolytica, and its effect on lipid accumulation was analysed. During fermentation, the dissolved oxygen (DO) concentration was controlled at 5, 10, 20 and 30 of full O saturation and also uncontrolled by varying the stirring speed and aeration rate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13111DOI Listing

Extracellular production of functional single-chain variable fragment against aflatoxin B using Escherichia coli.

Lett Appl Microbiol 2019 Mar 6;68(3):241-247. Epub 2019 Jan 6.

Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea.

scFv-BM3 is a single-chain variable fragment (scFv) against aflatoxin B (AFB ) engineered by affinity maturation and site-directed mutagenesis, and thus has a 31-fold higher affinity than its wild-type. To apply scFv-BM3 to immunological detection of AFB , periplasmic expression in Escherichia coli was attempted to produce a functional form of scFv-BM3. scFv-BM3 accumulated as inactive aggregates in the cells. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13110
Publisher Site
http://dx.doi.org/10.1111/lam.13110DOI Listing
March 2019
1 Read

A quantitative method to assess the role of indoor air decontamination to simultaneously reduce contamination of environmental surfaces: testing with vegetative and spore-forming bacteria.

Lett Appl Microbiol 2019 Mar 21;68(3):206-211. Epub 2019 Jan 21.

RB, Montvale, NJ, USA.

Indoor air can spread pathogens, which can be removed/inactivated by a variety of means in healthcare and other settings. We quantitatively assessed if air decontamination could also simultaneously reduce environmental surface contamination in the same setting. Two types of vegetative bacteria (Staphylococcus aureus and Acinetobacter baumannii), and a bacterial spore-former (Geobacillus stearothermophilus) were tested as representative airborne bacteria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13109DOI Listing

Development of real-time loop-mediated isothermal amplification (RealAmp) method for sensitive and rapid detection of pathogenic and nonpathogenic Leptospira.

Lett Appl Microbiol 2019 Feb;68(2):196-203

Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

Leptospirosis is a zoonotic disease that is spread through water contaminated with the spirochete Leptospira. In our study, RealAmp (real-time loop-mediated isothermal amplification) was developed to distinguish between pathogenic and nonpathogenic Leptospira spp. Melt curve analysis of RealAmp showed two distinct melt curves for pathogenic and nonpathogenic Leptospira spp with a Tm value of 90 ± 2 and 88 ± 2°C. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13108
Publisher Site
http://dx.doi.org/10.1111/lam.13108DOI Listing
February 2019
1 Read

Microbiota composition of dadih - a traditional fermented buffalo milk of West Sumatra.

Authors:
K Venema I S Surono

Lett Appl Microbiol 2019 Mar 31;68(3):234-240. Epub 2019 Jan 31.

Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta, Indonesia.

Dadih is an Indonesian traditional spontaneously fermented buffalo milk, produced in West-Sumatra, which is nutritious and has health benefits. The mechanism of action behind the health benefits is largely unknown, but several probiotic strains have been isolated from dadih, which may contribute to its health properties. To identify the composition of its microbiota, two artisanal dadih samples (n = 8) were collected from four producers. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13107
Publisher Site
http://dx.doi.org/10.1111/lam.13107DOI Listing
March 2019
4 Reads

Molecular characterization of virulence and antibiotic resistance genes among Salmonella serovars isolated from broilers in Egypt.

Lett Appl Microbiol 2019 Feb 6;68(2):188-195. Epub 2019 Jan 6.

Department of Zoonotic Diseases, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt.

This study was carried out to investigate the resistance to some antimicrobial groups among Salmonellae isolated from broilers in Egypt. The prevalence of some virulence and resistance genes among the recovered multidrug resistant (MDR) isolates was also scrutinized. A total of 55 (15·6%) Salmonella isolates were recovered from 353 different samples (liver, yolk sac, gall bladder and caecum), gathered from apparently healthy and diseased broilers suffered from diarrhoea, dehydration and respiratory distress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13106DOI Listing
February 2019

The action mechanism and biocontrol potentiality of novel isolates of Saccharomyces cerevisiae against the aflatoxigenic Aspergillus flavus.

Lett Appl Microbiol 2019 Feb 4;68(2):104-111. Epub 2019 Jan 4.

Botany & Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt.

Inhibition of Aspergillus flavus growth and its aflatoxins production using the biocontrol agent Saccharomyces cerevisiae as well as to explore its mode of action was studied. Eight strains of S. cerevisiae strains were able to suppress the growth of A. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13105DOI Listing
February 2019
1 Read

Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification.

Lett Appl Microbiol 2019 Feb 4;68(2):173-181. Epub 2019 Jan 4.

School of Chemical Engineering and Science, Swinburne University of Technology, Kuching, Sarawak, Malaysia.

Biocalcification through the use of ureolytic bacteria and biochemical activities has evolved in recent decades into a fervent resourceful effective technology suitable for soil stabilization, crack repair and bioremediation. Extensive studies have been carried out on numerous ureolytic bacterial species isolated from soils and sewage samples. However, very limited attention has been given to limestone caves with natural calcite formations as a possible source for isolation of ureolytic bacteria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13103DOI Listing
February 2019
2 Reads

Fibre degradation of wheat straw by Pleurotus erygnii under low moisture conditions during solid-state fermentation.

Lett Appl Microbiol 2019 Feb 2;68(2):182-187. Epub 2019 Jan 2.

School of Environment, Natural Resources and Geography, Bangor University, Bangor, Gwynedd, UK.

The application of solid-state fermentation offers an alternative to conventional, submerged approaches for a variety of bioconversion processes, including animal feeds, biofuels and fungal bioproducts. Optimizing solid-state fermentation under low moisture conditions could significantly impact the proportion of dry biomass that could be processed and improve the commercial viability of this approach, because of reduced input costs and higher yields of final products. Pleurotus erygnii that appeared to show tolerance to low moisture conditions was grown on saturated and desaturated wheat straw. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13104
Publisher Site
http://dx.doi.org/10.1111/lam.13104DOI Listing
February 2019
2 Reads

Endophytic Bacillus strains isolated from alfalfa (Medicago sativa L.) seeds: enhancing the lifespan of Caenorhabditis elegans.

Lett Appl Microbiol 2019 Mar 20;68(3):226-233. Epub 2019 Jan 20.

College of Life Sciences, Agricultural University of Hebei, Baoding, China.

Medicago sativa L. (alfalfa) is an important forage crop throughout the world. Despite the abiotic nutritional components of alfalfa having been extensively studied, there is only limited information on alfalfa endophytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13102DOI Listing
March 2019
1 Read

Effective biosynthesis of benzoyl-pyrrothine dithiolopyrrolone antibiotic by cinnamic acid-precursor addition in culture of Saccharothrix algeriensis NRRL B-24137.

Lett Appl Microbiol 2019 Feb 4;68(2):165-172. Epub 2019 Jan 4.

Laboratoire de Biologie des Systèmes Microbiens, Ecole Normale Supérieure de Kouba, Alger, Algeria.

Dithiolopyrrolone antibiotics, produced by several micro-organisms, are known for their strong antimicrobial and antitumor activities. Among of this micro-organisms, Saccharothrix algeriensis NRRL B-24137, a rare actinobacterium, has the ability to produce several dithiolopyrrolones derivatives depending on precursors added in the culture medium. After 10 days of strain fermentation on semi-synthetic medium supplemented with cinnamic acid and HPLC purification, biosynthesis of benzoyl-pyrrothine dithiolopyrrolone was evidenced through complete spectroscopic (UV-visible and 1H and 13C NMR) and spectrometric (electron impact mass spectrum) analyses. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13101
Publisher Site
http://dx.doi.org/10.1111/lam.13101DOI Listing
February 2019
1 Read

Coriander essential oil and linalool - interactions with antibiotics against Gram-positive and Gram-negative bacteria.

Lett Appl Microbiol 2019 Feb 4;68(2):156-164. Epub 2019 Jan 4.

Department of Pharmacognosy, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Iasi, Romania.

The aim of this study was to evaluate the antibacterial activity of coriander essential oil and its major constituent, linalool, in combination with antibiotics against Gram-positive (methicillin-susceptible and methicillin-resistant Staphylococcus aureus, S. epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli). The chemical composition of coriander essential oil was analyzed by gas chromatography with flame ionization and mass spectrometry detection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13100DOI Listing
February 2019
6 Reads

Occurrence of pathogenic Vibrio parahaemolyticus in seafood distribution channels and their antibiotic resistance profiles in S. Korea.

Lett Appl Microbiol 2019 Feb 4;68(2):128-133. Epub 2019 Jan 4.

Department of Food and Nutrition, Sookmyung Women's University, Seoul, Korea.

This study investigated the occurrence of Vibrio parahaemolyticus in seafood distribution channels, and the incidence of pathogenic and antibiotic-resistant strains. One hundred sixty-seven samples (126 fishery samples and 41 environmental samples) were collected from fishery auction markets, fish markets and online markets in S. Korea from March to September, 2017. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/lam.13099DOI Listing
February 2019
7 Reads

Arabinoxylan from Argentinian whole wheat flour promote the growth of Lactobacillus reuteri and Bifidobacterium breve.

Lett Appl Microbiol 2019 Feb 4;68(2):142-148. Epub 2019 Jan 4.

Instituto de Ciencia y Tecnología en Alimentos Córdoba, (ICYTAC-CONICET-UNC), Córdoba, Argentina.

Arabinoxylans are part of dietary fibre and have received attention given their emergent prebiotic character. Four arabinoxylans extracts were obtained from Argentinian soft and hard wheat. In vitro assays were performed to describe the extent to which the extracts from whole wheat flour support selective growth of Bifidobacterium breve and probiotic Lactobacillus reuteri ATCC23272 in a defined media. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13097
Publisher Site
http://dx.doi.org/10.1111/lam.13097DOI Listing
February 2019
6 Reads

Isolation and characterization of soil bacteria able to rapidly degrade the organophosphorus nematicide fosthiazate.

Lett Appl Microbiol 2019 Feb 2;68(2):149-155. Epub 2019 Jan 2.

Laboratory of Plant and Environmental Biotechnology, Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Larissa, Greece.

Foshtiazate is an organophosphorus nematicide commonly used in protected crops and potato plantations. It is toxic to mammals, birds and honeybees, it is persistent in certain soils and can be transported to water resources. Recent studies by our group demonstrated, for the first time, the development of enhanced biodegradation of fosthiazate in agricultural soils. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13098
Publisher Site
http://dx.doi.org/10.1111/lam.13098DOI Listing
February 2019
14 Reads

Effect of gamma irradiation on inactivation of Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes on pistachios.

Lett Appl Microbiol 2019 Jan 29;68(1):96-102. Epub 2018 Nov 29.

Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Center for Food Safety and Toxicology and Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul, Korea.

This study evaluated the efficacy of gamma irradiation to inactivate food-borne pathogens on pistachios (Pistacia vera L.). Pistachios inoculated with Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes were subjected to gamma irradiation in the range of 0, 0·5, 1, 3 and 5 kGy, and colour change was evaluated after treatment and during storage at room temperature. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13095
Publisher Site
http://dx.doi.org/10.1111/lam.13095DOI Listing
January 2019
14 Reads

Prevalence of Shiga toxin-producing Escherichia coli in pasture-based dairy herds.

Lett Appl Microbiol 2019 Feb 29;68(2):112-119. Epub 2018 Nov 29.

Food & Bio-based Products, AgResearch Ltd, Hopkirk Research Institute, Palmerston North, New Zealand.

Shiga toxin-producing Escherichia coli strains (STEC) are food-borne pathogens. While E. coli O157:H7 is commonly associated with cattle, less is known about the prevalence of non-O157 STEC serogroups in bovines. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13096
Publisher Site
http://dx.doi.org/10.1111/lam.13096DOI Listing
February 2019
6 Reads

Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing.

Lett Appl Microbiol 2019 Feb 29;68(2):120-127. Epub 2018 Nov 29.

Department of Dental Materials and Prosthodontics, Aracatuba Dental School, São Paulo State University (UNESP), Aracatuba, São Paulo, Brazil.

Conditions of the acrylic resin (AR) surface, such as roughness, can promote a favourable environment for the adhesion of micro-organisms, even on the surface of ocular prostheses. This study evaluated the influence of photopolymerized glaze application on the roughness of ARs and adhesion of Staphylococcus aureus and Staphylococcus epidermidis on ocular AR surfaces submitted to accelerated ageing. Two hundred and eighty-eight samples of white colour (N1) and colourless ARs were distributed in eight groups (n = 9), based on surface treatments (glaze or ARs submitted to only a final polishing), accelerated ageing (before and after) and periods of microbial growth (24- and 48-h). Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13094
Publisher Site
http://dx.doi.org/10.1111/lam.13094DOI Listing
February 2019
5 Reads

Identification and removal of contaminating microbial DNA from PCR reagents: impact on low-biomass microbiome analyses.

Lett Appl Microbiol 2019 Jan 23;68(1):2-8. Epub 2018 Nov 23.

Division of Obstetrics and Gynaecology, Faculty of Health & Medical Sciences, The University of Western Australia, Perth, WA, Australia.

Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to potential contamination introduced by PCR reagents. In this study, we assessed the proportion to which our DNA extraction kit and PCR master mix introduce contaminating microbial DNA to bacterial microbial profiles generated by 16S rRNA gene sequencing. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13091
Publisher Site
http://dx.doi.org/10.1111/lam.13091DOI Listing
January 2019
3 Reads

Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance.

Lett Appl Microbiol 2019 Jan 22;68(1):81-86. Epub 2018 Nov 22.

Department of Membrane Transport, Institute of Physiology of the Czech Academy of Sciences, Prague 4, Czech Republic.

Saccharomyces kudriavzevii is a nonconventional and rather osmosensitive yeast with a high potential of use in fermentation processes. To elucidate the basis of its relative osmosensitivity, the role of the STL1 gene encoding a putative glycerol uptake system was studied. Under higher osmotic pressure, the addition of a low amount of glycerol to the growth medium improved the growth of S. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13093
Publisher Site
http://dx.doi.org/10.1111/lam.13093DOI Listing
January 2019
1 Read

The antibiotics potentiator bicarbonate causes upregulation of tryptophanase and iron acquisition proteins in Escherichia coli.

Lett Appl Microbiol 2019 Jan 26;68(1):87-95. Epub 2018 Nov 26.

Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.

We have reported that bicarbonate (NaHCO ) potentiates the activity of aminoglycosides in Escherichia coli, but the action mechanism was not identified. To eventually understand how NaHCO can potentiate antibiotics, we thought that a rational first step was to examine the effect of NaHCO separately and to inspect initial gene expression changes triggered by it. In this work, we started by confirming that NaHCO can reduce the number of viable E. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13092
Publisher Site
http://dx.doi.org/10.1111/lam.13092DOI Listing
January 2019
8 Reads

Biotransformation of ginsenoside Rb1 to Gyp-XVII and minor ginsenoside Rg3 by endophytic bacterium Flavobacterium sp. GE 32 isolated from Panax ginseng.

Authors:
Y Fu

Lett Appl Microbiol 2019 Feb 6;68(2):134-141. Epub 2019 Jan 6.

College of Chemistry and Life Science, Anshan Normal University, Anshan, China.

The rare ginsenoside Rg3 is attracting more attention because of its good physiological activity and urgent need. There are many pathways to obtain ginsenoside Rg3, including chemical and biological methods. Among these, the conversion of the protopanaxadiol-type ginsenosides by microbial hydrolysis is a trend due to its high efficiency and mild conditions. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/lam.13090
Publisher Site
http://dx.doi.org/10.1111/lam.13090DOI Listing
February 2019
10 Reads