5,283 results match your criteria Legionnaires Disease


effector RavD binds phosphatidylinositol-3-phosphate and helps suppress endolysosomal maturation of the -containing vacuole.

J Biol Chem 2019 Feb 7. Epub 2019 Feb 7.

Biological Sciences, University of Delaware, United States.

Upon phagocytosis into macrophages, the intracellular bacterial pathogen secretes effector proteins that manipulate host cell components, enabling it to evade lysosomal degradation. However, the bacterial proteins involved in this evasion are incompletely characterized. Here, we show that the effector protein RavD targets host membrane compartments and contributes to the molecular mechanism that the pathogen uses to prevent encounters with lysosomes. Read More

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http://dx.doi.org/10.1074/jbc.RA118.007086DOI Listing
February 2019
1 Read

A versatile de novo synthesis of legionaminic acid and 4-epi-legionaminic acid starting from d-serine.

Carbohydr Res 2019 Jan 23;474:34-42. Epub 2019 Jan 23.

AG Schmid, Institute of Organic Chemistry, University of Vienna, Währinger Straße 38, A-1090, Vienna, Austria.

Legionaminic acid and 4-epi-legionaminic acid are 5,7-diacetamido nonulosonic acids and are assumed to play a crucial role in the virulence of Legionella pneumophila, the causative agent of Legionnaires' disease. Moreover, they are ideal target motifs for the development of vaccines and pathogen detection. Herein, we present a versatile de novo synthesis of legionaminic acid and 4-epi-legionaminic acid. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00086215183073
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http://dx.doi.org/10.1016/j.carres.2019.01.009DOI Listing
January 2019
3 Reads

Comparison of Legiolert and a Conventional Culture Method for Detection of from Cooling Towers in Québec.

J AOAC Int 2019 Feb 1. Epub 2019 Feb 1.

EnvironeX Group, 2325 Fernand-Lafontaine Blvd, Longueuil, QC J4N 1N7, Canada.

: Legionnaires' disease is a potentially lethal pneumonia contracted through inhalation of aerosolized water contaminated with bacteria. Detection and control of , the primary species responsible for the disease, is critical to public health. In Québec, cooling towers and evaporative condensers are required to follow a maintenance and testing program to ensure concentrations remain at acceptable levels. Read More

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http://dx.doi.org/10.5740/jaoacint.18-0245DOI Listing
February 2019
11 Reads

Air-conditioner cooling towers as complex reservoirs and continuous source of infection evidenced by a genomic analysis study in 2017, Switzerland.

Euro Surveill 2019 Jan;24(4)

Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.

IntroductionWater supply and air-conditioner cooling towers (ACCT) are potential sources of infection in people. During outbreaks, traditional typing methods cannot sufficiently segregate strains to reliably trace back transmissions to these artificial water systems. Moreover, because multiple strains may be present within these systems, methods to adequately distinguish strains are needed. Read More

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http://dx.doi.org/10.2807/1560-7917.ES.2019.24.4.1800192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351994PMC
January 2019
1 Read

The Mouse as a Model for Pulmonary Legionella Infection.

Methods Mol Biol 2019 ;1921:399-417

Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, VIC, Australia.

Infection of C57BL/6 mice with wild-type Legionella pneumophila typically results in very mild disease. However, in mice where the cytosolic recognition of flagellin is impaired by mutation, L. pneumophila infection results in more severe lung inflammation that is reminiscent of Legionnaires' disease. Read More

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http://dx.doi.org/10.1007/978-1-4939-9048-1_25DOI Listing
January 2019
1 Read

Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila.

Methods Mol Biol 2019 ;1921:323-331

Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany.

Legionnaires' disease is a severe pneumonia caused by inhalation of Legionella pneumophila. Although powerful infection models ranging from monocellular host systems to mammals were developed, numerous intra- and extracellular interactions of L. pneumophila factors with human lung tissue structures remain unknown. Read More

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http://link.springer.com/10.1007/978-1-4939-9048-1_21
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http://dx.doi.org/10.1007/978-1-4939-9048-1_21DOI Listing
January 2019
4 Reads

Genetic Manipulation of Non-pneumophila Legionella: Protocols Developed for Legionella longbeachae.

Methods Mol Biol 2019 ;1921:145-157

Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.

Current biomedical research into Legionnaires' disease is dominated by studies of Legionella pneumophila, largely because this pathogen is responsible for approximately 90% of clinical disease worldwide. However, in certain geographical regions, infections with non-pneumophila species are responsible for a significant proportion of diagnosed Legionnaires' disease. Understanding the pathogenesis of these non-pneumophila species of Legionella is an important step toward clinical intervention. Read More

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http://dx.doi.org/10.1007/978-1-4939-9048-1_9DOI Listing
January 2019
2 Reads

Screening Targeted Legionella pneumophila Mutant Libraries In Vivo Using INSeq.

Methods Mol Biol 2019 ;1921:123-144

Division of Biology, Kansas State University, Manhattan, KS, USA.

Legionella pneumophila is an intracellular bacterial pathogen that can cause a severe inflammatory pneumonia in humans called Legionnaires' disease, which results from bacterial replication within alveolar macrophages. L. pneumophila replication within macrophages is dependent on hundreds of individual protein virulence factors. Read More

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http://dx.doi.org/10.1007/978-1-4939-9048-1_8DOI Listing
January 2019
1 Read

Migration of Acanthamoeba castellanii Through Legionella Biofilms.

Methods Mol Biol 2019 ;1921:79-89

Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland.

The amoeba-resistant bacterium Legionella pneumophila infects humans through aerosols and thereby can cause a life-threatening pneumonia termed Legionnaires' disease. In the environment L. pneumophila forms and colonizes biofilms, which usually comprise complex multispecies communities. Read More

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http://dx.doi.org/10.1007/978-1-4939-9048-1_5DOI Listing
January 2019
1 Read

Draft Genome Sequences of Legionella taurinensis Recovered from a Hot Water System in Austria, 2018.

Microbiol Resour Announc 2019 Jan 17;8(3). Epub 2019 Jan 17.

Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Vienna, Austria.

Members of the genus are widespread in natural water sources. This is the first report on the draft genome sequences of Legionella taurinensis in Austria. The isolates were recovered from a hot water system associated with a case of Legionnaires' disease caused by Legionella pneumophila in 2018. Read More

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http://dx.doi.org/10.1128/MRA.01478-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346165PMC
January 2019
1 Read

Cardiovascular events and de novo AF following Legionnaires disease.

Eur J Intern Med 2019 Jan 24. Epub 2019 Jan 24.

Department of Intensive Care Medicine, Centro Hospitalar São João, Portugal.

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http://dx.doi.org/10.1016/j.ejim.2019.01.010DOI Listing
January 2019
1 Read

Using an optimal set of features with a machine learning-based approach to predict effector proteins for Legionella pneumophila.

PLoS One 2019 25;14(1):e0202312. Epub 2019 Jan 25.

School of Electrical Engineering and Computer Science, Washington State University, Pullman, Washington, United States of America.

Type IV secretion systems exist in a number of bacterial pathogens and are used to secrete effector proteins directly into host cells in order to change their environment making the environment hospitable for the bacteria. In recent years, several machine learning algorithms have been developed to predict effector proteins, potentially facilitating experimental verification. However, inconsistencies exist between their results. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202312PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347213PMC
January 2019
1 Read

Legionella SBT applied directly to respiratory samples as a rapid molecular epidemiological tool.

Sci Rep 2019 Jan 24;9(1):623. Epub 2019 Jan 24.

Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.

Legionnaires' disease (LD) is an atypical pneumonia caused by the inhalation of Legionella. The methods used for the diagnosis of LD are direct culture of respiratory samples and urinary antigen detection. However, the sensitivity of culture is low, and the urinary antigen test is specific only for L. Read More

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http://dx.doi.org/10.1038/s41598-018-36924-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346096PMC
January 2019
2 Reads

The International Collaborative on Fatigue Following Infection (COFFI).

Fatigue 2018 19;6(2):106-121. Epub 2018 Jan 19.

Kirby Institute, University of New South Wales, Sydney, New South Wales, Australia.

Background: The purpose of the Collaborative on Fatigue Following Infection (COFFI) is for investigators of post-infection fatigue (PIF) and other syndromes to collaborate on these enigmatic and poorly understood conditions by studying relatively homogeneous populations with known infectious triggers. Utilizing COFFI, pooled data and stored biosamples will support both epidemiological and laboratory research to better understand the etiology and risk factors for development and progression of PIF.

Methods: COFFI consists of prospective cohorts from the UK, Netherlands, Norway, USA, New Zealand and Australia, with some cohorts closed and some open to recruitment. Read More

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https://www.tandfonline.com/doi/full/10.1080/21641846.2018.1
Publisher Site
http://dx.doi.org/10.1080/21641846.2018.1426086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333416PMC
January 2018
5 Reads

Controlling Legionella pneumophila in water systems at reduced hot water temperatures with copper and silver ionization.

Am J Infect Control 2019 Jan 17. Epub 2019 Jan 17.

Department of Microbiology, Virology, and Infection Prevention Control, Great Ormond Street Hospital NHS Foundation Trust, London, United Kingdom.

Background: Hospital-acquired Legionnaires' disease is associated with the presence of Legionella pneumophila in hospital water systems. In the United Kingdom, the Department of Health recommends maintaining hot water temperatures >55°C and cold water temperatures <20°C at the point of delivery to prevent proliferation of L pneumophila in water systems. In this study, we evaluated the efficacy of copper and silver ionization to control L pneumophila at deliberately reduced hot water temperatures (43°C) within a newly installed water system in a new building linked to a large health care facility in the United Kingdom. Read More

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http://dx.doi.org/10.1016/j.ajic.2018.12.005DOI Listing
January 2019
4 Reads

Legionnaires Disease Surveillance in US Department of Veterans Affairs Medical Facilities and Assessment of Health Care Facility Association.

JAMA Netw Open 2018 Jun 1;1(2):e180230. Epub 2018 Jun 1.

National Infectious Diseases Service, Specialty Care Services, Veterans Health Administration, Department of Veterans Affairs, Washington, DC.

Importance: Legionnaires disease (LD) incidence is increasing in the United States. Health care facilities are a high-risk setting for transmission of Legionella bacteria from building water systems to occupants. However, the contribution of LD in health care facilities to national LD rates is not well characterized. Read More

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http://dx.doi.org/10.1001/jamanetworkopen.2018.0230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324594PMC
June 2018
1 Read

Prevention of Health Care-Associated Legionnaires Disease.

Authors:
Shawn J Skerrett

JAMA Netw Open 2018 Jun 1;1(2):e180232. Epub 2018 Jun 1.

Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Washington School of Medicine, Seattle.

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http://dx.doi.org/10.1001/jamanetworkopen.2018.0232DOI Listing
June 2018
1 Read

Overview and comparison of Legionella regulations worldwide.

Am J Infect Control 2019 Jan 9. Epub 2019 Jan 9.

Research Group Building Physics, Construction and Climate Control, Department of Architecture and Urban Planning, Ghent University, Ghent, Belgium.

Background: Legionnaires disease occurs worldwide. Many authorities have guidelines and regulations to prevent and control Legionella in water systems. These regulations are based on often very limited field and laboratory observations and measurements. Read More

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http://dx.doi.org/10.1016/j.ajic.2018.10.006DOI Listing
January 2019
2 Reads

Therapeutic hypothermia as an adjunct to extracorporeal membrane oxygenation for acute respiratory distress and refractory hypoxemia.

Perfusion 2019 Jan 10:267659118822941. Epub 2019 Jan 10.

2 Division of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, Canada.

Legionella community-acquired pneumonia necessitating veno-venous extracorporeal membrane oxygenation for severe acute respiratory distress syndrome has been reported in adults. However, few options remain in cases of refractory hypoxemia on veno-venous extracorporeal membrane oxygenation. Herein, we describe adjunctive extended therapeutic hypothermia for refractory hypoxemia despite veno-venous extracorporeal membrane oxygenation for successful management of severe acute respiratory distress syndrome secondary to Legionella. Read More

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http://dx.doi.org/10.1177/0267659118822941DOI Listing
January 2019
2 Reads
1.083 Impact Factor

New approach to environmental investigation of an explosive legionnaires´ disease outbreak in Spain: early identification of potential risk sources by rapid Legionella spp immunosensing technique.

BMC Infect Dis 2018 Dec 27;18(1):696. Epub 2018 Dec 27.

Ministry of Health of the Council of Communities of Castilla - La Mancha, Av. de Madrid s/n, 45600 Talavera de la Reina, Toledo, Spain.

Background: An explosive outbreak of Legionnaires' disease (LD) was identified on 11 December 2015 in Manzanares, Ciudad Real, Spain, and was declared closed by 03 February 2016. The number of declared cases was 593 with 277 confirmed cases so that it can be considered as one of the outbreaks with highest attack rate. This rate could be attributed to the ageing of the population, among others, in addition to various risk factors and habits, and the meteorological conditions (thermal inversion) maintained in this municipality at the time. Read More

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http://dx.doi.org/10.1186/s12879-018-3605-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307211PMC
December 2018
2 Reads

The effector LtpM is a new type of phosphoinositide-activated glucosyltransferase.

J Biol Chem 2018 Dec 20. Epub 2018 Dec 20.

Centre for Experimental Medicine, Queen's University Belfast, United Kingdom.

causes Legionnaires' disease, a severe form of pneumonia. translocates more than 300 effectors into host cells via its Dot/Icm type IV secretion system to enable its replication in target cells. Here, we studied the effector LtpM, which is encoded in a recombination hot spot in Paris. Read More

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http://dx.doi.org/10.1074/jbc.RA118.005952DOI Listing
December 2018
1 Read

Improvement of Legionnaires' disease diagnosis using real-time PCR assay: a retrospective analysis, Italy, 2010 to 2015.

Euro Surveill 2018 Dec;23(50)

Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.

AimTo evaluate real-time PCR as a diagnostic method for Legionnaires' disease (LD). Detection of DNA is among the laboratory criteria of a probable LD case, according to the European Centre for Disease Prevention and Control, although the utility and advantages, as compared to culture, are widely recognised.MethodsTwo independent laboratories, one using an in-house and the other a commercial real-time PCR assay, analysed 354 respiratory samples from 311 patients hospitalised with pneumonia between 2010-15. Read More

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http://dx.doi.org/10.2807/1560-7917.ES.2018.23.50.1800032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299505PMC
December 2018
2 Reads

A community outbreak of Legionnaires' disease in Geneva, Switzerland, June to September 2017.

Swiss Med Wkly 2018 11 2;148:w14687. Epub 2018 Dec 2.

Laboratory of Virology, Division of Laboratory Medicine and Division of Infectious Diseases, University of Geneva Hospitals, Switzerland; University of Geneva Medical School, Switzerland.

Purpose: Eight confirmed cases of Legionnaires’ disease were identified at the Geneva University Hospitals between 28 July 2017 and 02 August 2017, leading to a detailed outbreak investigation.

Methods: Legionnaires’ disease cases were defined according to Swiss and European (ELDSNet) consensus guidelines. An outbreak investigation task force was put in place. Read More

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http://dx.doi.org/10.4414/smw.2018.14687DOI Listing
November 2018
2 Reads

Induction of protective immunity by recombinant peptidoglycan associated lipoprotein (rPAL) protein of Legionella pneumophila in a BALB/c mouse model.

Microb Pathog 2019 Mar 11;128:100-105. Epub 2018 Dec 11.

Department of Microbiology, Faculty of Advanced Sciences, Medical Sciences University Islamic Azad, Tehran, Iran.

Legionella pneumophila causes a severe form of pneumonia known as Legionnaires' disease especially in patients with impaired cellular immune response. In order to prevent the disease, immunogenicity and the level of the induction of protective immunity from the recombinant peptidoglycan-associated lipoprotein (rPAL) against Legionella pneumophila in BALB/c mice was examined. Mice immunized with (rPAL) rapidly increased an antibody response in serum and also displayed a strong activation of both innate and adaptive cell-mediated immunity as determined by antigen-specific splenocyte proliferation, an early production of pro-inflammatory cytokines in the serum and in the splenocyte cultures. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08824010183119
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http://dx.doi.org/10.1016/j.micpath.2018.12.014DOI Listing
March 2019
8 Reads
2.000 Impact Factor

-Containing Vacuoles Capture PtdIns(4)-Rich Vesicles Derived from the Golgi Apparatus.

MBio 2018 12 11;9(6). Epub 2018 Dec 11.

Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

is the causative agent of a pneumonia termed Legionnaires' disease. The facultative intracellular bacterium employs the Icm/Dot type IV secretion system (T4SS) and a plethora of translocated "effector" proteins to interfere with host vesicle trafficking pathways and establish a replicative niche, the -containing vacuole (LCV). Internalization of the pathogen and the events immediately ensuing are accompanied by host cell-mediated phosphoinositide (PI) lipid changes and the Icm/Dot-controlled conversion of the LCV from a PtdIns(3)-positive vacuole into a PtdIns(4)-positive replication-permissive compartment, which tightly associates with the endoplasmic reticulum. Read More

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http://dx.doi.org/10.1128/mBio.02420-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299486PMC
December 2018
15 Reads

Probable reinfection with Legionella pneumophila - A case report.

Int J Hyg Environ Health 2018 Nov 27. Epub 2018 Nov 27.

Robert Koch Institute, Seestr. 10, Berlin, Germany. Electronic address:

In Germany community-acquired Legionnaires' disease is usually caused by the species Legionella pneumophila. Recurrent cases of Legionnaires' disease are rarely reported and are due either to a second infection (reinfection) or a relapse of a previous case. We report a case of recurrent Legionnaires' disease in an 86-year-old female patient infected with Legionella pneumophila serogroup 1, monoclonal antibody-subtype Knoxville, sequence type unknown. Read More

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http://dx.doi.org/10.1016/j.ijheh.2018.11.001DOI Listing
November 2018
1 Read

Travel-associated Legionnaires' disease: would changing cluster definition lead to the prevention of a larger number of cases?

Epidemiol Infect 2018 Dec 3:1-6. Epub 2018 Dec 3.

Biolinea Int.,Palma de Mallorca,Spain.

According to European Guidelines for Legionnaires' Disease prevention and control, travel-associated Legionnaires' disease (TALD) cases are managed differently if classified as sporadic or as part of a cluster and more stringent control measures are deployed after clusters are identified. In this study, we propose to modify the current cluster definition: 'two or more cases of Legionnaires' disease (LD) who stayed at, or visited, the same commercial accommodation site 2-10 days before onset of illness and whose onset is within the same 2-year period' with a new cluster definition, i.e. Read More

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http://dx.doi.org/10.1017/S0950268818003266DOI Listing
December 2018
2 Reads

Legionella Colonization of Hotel Water Systems in Touristic Places of Greece: Association with System Characteristics and Physicochemical Parameters.

Int J Environ Res Public Health 2018 11 30;15(12). Epub 2018 Nov 30.

Department of Hygiene and Epidemiology, Medical School, University of Thessaly, 41222 Larissa, Greece.

This study aimed to assess the colonization of hotel water systems in central Greece and Corfu by , and to investigate the association between physicochemical parameters and colonization. Standardized hygiene inspection was conducted in 51 hotels, and 556 water samples were analyzed for spp Free chlorine concentration, pH, hardness, conductivity, and trace metals were defined in cold water samples. The results of inspections and chemical analyses were associated with the microbiological results using univariate and logistic regression analysis. Read More

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http://dx.doi.org/10.3390/ijerph15122707DOI Listing
November 2018
3 Reads

Legionella occurrence in municipal and industrial wastewater treatment plants and risks of reclaimed wastewater reuse: Review.

Water Res 2019 Feb 1;149:21-34. Epub 2018 Nov 1.

Leibniz University Hannover, Institute for Sanitary Engineering and Waste Management, Hannover, 30167, Germany. Electronic address:

Wastewater treatment plants (WWTPs) have been identified as confirmed but until today underestimated sources of Legionella, playing an important role in local and community cases and outbreaks of Legionnaires' disease. In general, aerobic biological systems provide an optimum environment for the growth of Legionella due to high organic nitrogen and oxygen concentrations, ideal temperatures and the presence of protozoa. However, few studies have investigated the occurrence of Legionella in WWTPs, and many questions in regards to the interacting factors that promote the proliferation and persistence of Legionella in these treatment systems are still unanswered. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00431354183089
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http://dx.doi.org/10.1016/j.watres.2018.10.080DOI Listing
February 2019
17 Reads

and Legionnaires' disease: Time to explore in India.

Indian J Med Microbiol 2018 Jul-Sep;36(3):324-333

Department of Microbiology, Indira Gandhi Medical College and Research Institute, Puducherry, India.

Legionella pneumophila was first recognised as a fatal cause of pneumonia more than four decades ago, during the 1976-American Legion convention in Philadelphia, USA. Legionella spp. continue to cause disease outbreaks of public health significance, and at present, Legionnaires' disease (LD) has emerged as an important cause of community and hospital-acquired pneumonia. Read More

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http://dx.doi.org/10.4103/ijmm.IJMM_18_298DOI Listing
November 2018
1 Read

Highlighting the Potency of Biosurfactants Produced by Strains as Anti- Agents.

Biomed Res Int 2018 22;2018:8194368. Epub 2018 Oct 22.

Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Université de Poitiers, France.

, the causative agent of Legionnaires' disease, is a waterborne bacterium mainly found in man-made water systems in close association with free-living amoebae and multispecies biofilms. strains, originating from various environments including freshwater systems or isolated from hospitalized patients, were tested for their antagonistic activity towards . A high amount of tested strains was thus found to be active. Read More

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http://dx.doi.org/10.1155/2018/8194368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217892PMC
October 2018
2 Reads

Legionella feeleii: pneumonia or Pontiac fever? Bacterial virulence traits and host immune response.

Med Microbiol Immunol 2019 Feb 1;208(1):25-32. Epub 2018 Nov 1.

School of Basic Medical Science, Hebei Medical University, Shijiazhuang, 050017, People's Republic of China.

Gram-negative bacterium Legionella is able to proliferate intracellularly in mammalian host cells and amoeba, which became known in 1976 since they caused a large outbreak of pneumonia. It had been reported that different strains of Legionella pneumophila, Legionella micdadei, Legionella longbeachae, and Legionella feeleii caused human respiratory diseases, which were known as Pontiac fever or Legionnaires' disease. However, the differences of the virulence traits among the strains of the single species and the pathogenesis of the two diseases that were due to the bacterial virulence factors had not been well elucidated. Read More

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http://link.springer.com/10.1007/s00430-018-0571-0
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http://dx.doi.org/10.1007/s00430-018-0571-0DOI Listing
February 2019
4 Reads

Genomic heterogeneity differentiates clinical and environmental subgroups of Legionella pneumophila sequence type 1.

PLoS One 2018 18;13(10):e0206110. Epub 2018 Oct 18.

Pneumonia Response and Surveillance Laboratory, Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.

Legionella spp. are the cause of a severe bacterial pneumonia known as Legionnaires' disease (LD). In some cases, current genetic subtyping methods cannot resolve LD outbreaks caused by common, potentially endemic L. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0206110PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193728PMC
October 2018
7 Reads

Simulation of Legionnaires' disease prospective spatiotemporal cluster detection, Allegheny County, Pennsylvania, USA.

Epidemiol Infect 2018 Oct 18:1-9. Epub 2018 Oct 18.

New York City Department of Health and Mental Hygiene,Bureau of Communicable Disease,Queens, New York,USA.

Legionnaires' disease (LD) incidence in the USA has quadrupled since 2000. Health departments must detect LD outbreaks quickly to identify and remediate sources. We tested the performance of a system to prospectively detect simulated LD outbreaks in Allegheny County, Pennsylvania, USA. Read More

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http://dx.doi.org/10.1017/S0950268818002789DOI Listing
October 2018
2 Reads

Peptidyl-Prolyl-/-Isomerases Mip and PpiB of Contribute to Surface Translocation, Growth at Suboptimal Temperature, and Infection.

Infect Immun 2019 Jan 19;87(1). Epub 2018 Dec 19.

Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany

The gammaproteobacterium is the causative agent of Legionnaires' disease, an atypical pneumonia that manifests itself with severe lung damage. , a common inhabitant of freshwater environments, replicates in free-living amoebae and persists in biofilms in natural and man-made water systems. Its environmental versatility is reflected in its ability to survive and grow within a broad temperature range as well as its capability to colonize and infect a wide range of hosts, including protozoa and humans. Read More

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http://dx.doi.org/10.1128/IAI.00939-17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300627PMC
January 2019
2 Reads

The Importance of Revisiting Legionellales Diversity.

Trends Parasitol 2018 12 12;34(12):1027-1037. Epub 2018 Oct 12.

Université de Poitiers, Laboratoire Ecologie et Biologie des Interactions - UMR CNRS 7267, F-86073 Poitiers, France.

Bacteria of the order Legionellales, such as Legionella pneumophila, the agent of Legionnaires' disease, and Coxiella burnetii, the agent of Q fever, are widely recognized as human pathogens. While our view of the Legionellales is often limited to clinical isolates, ecological surveys are continually uncovering new members of the Legionellales that do not fall into the recognized pathogenic species. Here we emphasize that most of these Legionellales are nonpathogenic forms that have evolved symbiotic lifestyles with nonvertebrate hosts. Read More

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http://dx.doi.org/10.1016/j.pt.2018.09.008DOI Listing
December 2018
2 Reads

Spatial structuring of a Legionella pneumophila population within the water system of a large occupational building.

Microb Genom 2018 Oct 12;4(10). Epub 2018 Oct 12.

1​Pathogen Genomics, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.

The diversity of Legionella pneumophila populations within single water systems is not well understood, particularly in those unassociated with cases of Legionnaires' disease. Here, we performed genomic analysis of 235 L. pneumophila isolates obtained from 28 water samples in 13 locations within a large occupational building. Read More

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http://www.microbiologyresearch.org/content/journal/mgen/10.
Publisher Site
http://dx.doi.org/10.1099/mgen.0.000226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249432PMC
October 2018
7 Reads

Preventing hospital-acquired Legionnaires' disease: A snapshot of clinical practices and water management approaches in US acute-care hospitals.

Infect Control Hosp Epidemiol 2018 Dec 8;39(12):1470-1472. Epub 2018 Oct 8.

1Rollins School of Public Health,Emory University,Atlanta,Georgia.

In 2017, we surveyed 101 SHEA Research Network hospitals regarding Legionnaires' disease (LD). Of 29 respondents, 94% have or are developing a water management plan with varying characteristics and personnel engaged. Most LD diagnostic testing is limited to urine antigen testing. Read More

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http://dx.doi.org/10.1017/ice.2018.240DOI Listing
December 2018
4 Reads

Editorial: Biology and Pathogenesis of .

Front Cell Infect Microbiol 2018 19;8:328. Epub 2018 Sep 19.

Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States.

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http://dx.doi.org/10.3389/fcimb.2018.00328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156375PMC
September 2018
13 Reads

The Growing Impact of Legionella in the Flint Water Crisis.

J Natl Black Nurses Assoc 2018 Jul;29(1):44-50

University of Michigan-Flint School of Nursing, 2180 William S. White Building, 303 E. Kearsley Street, Flint, MI 48502- 1950.

The water crisis in Flint, Michigan raised national awareness about lead- tainted drinking water, particularly its harm to children and pregnant and lactating women. However, according to recent findings, there may be another by-product of this man-made crisis. Failure to use anticorrosive material in the water, a practice mandated by the 1978 Clean Water Act, is being linked to an outbreak of Legionnaires' disease, an illness detrimental primarily to adults over 65 years of age. Read More

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July 2018
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Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics.

Methods Mol Biol 2018 ;1841:59-76

Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland.

The causative agent of Legionnaires' disease, Legionella pneumophila, replicates in free-living amoebae as well as in macrophages of the innate immune system within a distinct membrane-bound compartment, the "Legionella-containing-vacuole" (LCV). LCV formation is a complex process and requires the bacterial Icm/Dot type IV secretion system, which translocates approximately 300 different "effector" proteins. Intact LCVs from infected Dictyostelium discoideum amoebae or RAW 264. Read More

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http://dx.doi.org/10.1007/978-1-4939-8695-8_6DOI Listing
January 2018
5 Reads

Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins.

Methods Mol Biol 2018 ;1844:33-38

Department of Biological Sciences, Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN, USA.

Ubiquitination is one of the most important posttranslational modifications in eukaryotic cells where it regulates the activity, cellular localization, and half-life of proteins. Ubiquitination thus affects many essential cellular processes, including vesicle trafficking, cell cycle, DNA repair, immune response, and protein homeostasis. The ubiquitin system is exclusive to eukaryotes; however, pathogenic bacteria have developed effective strategies to influence the host ubiquitin system for their own benefit. Read More

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http://dx.doi.org/10.1007/978-1-4939-8706-1_3DOI Listing
January 2018
14 Reads

The virulence of Legionella pneumophila is positively correlated with its ability to stimulate NF-κB activation.

Future Microbiol 2018 09 21;13:1247-1259. Epub 2018 Sep 21.

CAS Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, PR China.

Aim: Our work is to study the correlation between the virulence of different Legionella pneumophila in mouse model and its ability to activate NF-κB signaling pathway in vitro.

Materials & Methods: We measured the abilities of different strains of L. pneumophila to induce the activation of NF-κB signaling pathway in vitro. Read More

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http://dx.doi.org/10.2217/fmb-2018-0051DOI Listing
September 2018
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Two Community Clusters of Legionnaires' Disease Directly Linked to a Biologic Wastewater Treatment Plant, the Netherlands.

Emerg Infect Dis 2018 10;24(10):1914-1918

A biologic wastewater treatment plant was identified as a common source for 2 consecutive Legionnaires' disease clusters in the Netherlands in 2016 and 2017. Sequence typing and transmission modeling indicated direct and long-distance transmission of Legionella, indicating this source type should also be investigated in sporadic Legionnaires' disease cases. Read More

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http://dx.doi.org/10.3201/eid2410.180906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154163PMC
October 2018
6 Reads

Disseminated extrapulmonary Legionella pneumophila infection presenting with panniculitis: case report and literature review.

BMC Infect Dis 2018 Sep 17;18(1):467. Epub 2018 Sep 17.

Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 270 Rama VI Road, Ratchathewi District, Bangkok, Thailand.

Background: Legionellosis is a well-known cause of pneumonia. Primary cutaneous and subcutaneous infection caused by Legionella pneumophila is rare and the diagnosis is challenging.

Case Presentation: A 38-year-old Thai woman with systemic lupus erythematosus and myasthenia gravis treated with prednisolone and azathioprine presented to our hospital with low-grade fever, diarrhea, and indurated skin lesions on both thighs. Read More

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http://dx.doi.org/10.1186/s12879-018-3378-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142325PMC
September 2018
2 Reads

Clusters of Healthcare-Associated Legionnaires' Disease in Two Hospitals of Central Greece.

Case Rep Infect Dis 2018 15;2018:2570758. Epub 2018 Aug 15.

Department of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece.

Healthcare-associated Legionnaires' disease often leads to fatal respiratory tract infection among hospitalized patients. In this report, three cases of Legionnaires' disease among patients in two different hospitals (Hospital A and Hospital B) were investigated. After conducting an epidemiologic and environmental investigation, the water distribution systems (WDSs) were identified as the possible source of infection, as serogroup 1 (1) was isolated from both clinical and environmental samples. Read More

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http://dx.doi.org/10.1155/2018/2570758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114232PMC
August 2018
14 Reads

Author Response to Comments on "Rolling epidemic of Legionnaires' disease outbreaks in small geographic areas".

Emerg Microbes Infect 2018 Sep 5;7(1):151. Epub 2018 Sep 5.

School of Public Health and Community Medicine, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.

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http://dx.doi.org/10.1038/s41426-018-0150-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123464PMC
September 2018
2 Reads

Comments on "Rolling epidemic of Legionnaires' disease outbreaks in small geographic areas".

Emerg Microbes Infect 2018 Sep 5;7(1):150. Epub 2018 Sep 5.

Bureau of Communicable Disease, New York City Department of Health and Mental Hygiene, Long Island City, NY, 11101, USA.

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http://dx.doi.org/10.1038/s41426-018-0152-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123401PMC
September 2018
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Immunosuppressive Treatment and Its Effect on the Occurrence of Pneumocystis jiroveci, Mycoplasma pneumoniae, Chlamydophila pnemoniae, and Legionella pneumophila Infections/Colonizations Among Lung Transplant Recipients.

Transplant Proc 2018 Sep 13;50(7):2053-2058. Epub 2018 Mar 13.

Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, Medical University of Silesia in Katowice, Silesian Center for Heart Diseases, Zabrze, Poland. Electronic address:

Background: The aim of the study was to assess the frequency of infections caused by Pneumocystis jiroveci, Chlamydophila pneumoniae, Legionella pneumophila, and Mycoplasma pneumoniae among lung transplant recipients in the context of immunosuppression.

Methods: The study group consisted of 94 patients (37 women and 57 men; mean age 42.03 years) transplanted between 2009 and 2016 at the Silesia Center for Heart Diseases (SCCS). Read More

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http://dx.doi.org/10.1016/j.transproceed.2017.12.059DOI Listing
September 2018
19 Reads