1,815 results match your criteria Legal Medicine [Journal]


LC-MS/MS analysis of two new designer drugs (FLY serie) in rat plasma and its application to a pharmacokinetic study.

Leg Med (Tokyo) 2019 Apr 10;38:58-63. Epub 2019 Apr 10.

Dipartimento di Chimica e Farmacia, Università di Sassari, 07100 Sassari, Italy.

The widespread diffusion of new psychoactive substances, requires a continuous update and development of new methods able to identify and quantify these new molecules in biological matrices. In this study an analytical method for the determination of two new benzodifuranyl derivatives, 1-(2,3,6,7-tetrahydrofuro[2,3-f][1]benzofuran-4-yl)propan-2-amine and 2-(2,3,6,7-tetrahydrofuro[2,3-f][1]benzofuran-4-yl)ethanamine, in rat plasma was developed. A solid phase extraction using C18 cartridges was carried out obtaining good recoveries with low matrix effect. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.04.004DOI Listing
April 2019
2 Reads

Using miRNAs and circRNAs to estimate PMI in advanced stage.

Leg Med (Tokyo) 2019 Apr 2;38:51-57. Epub 2019 Apr 2.

Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address:

In our previous study, we evaluated the stability of multi-RNA markers in heart, liver and skeletal muscle tissues of mice within 8 days after death and concluded that microRNAs (miRNAs) and circular (circRNAs) were more stable as reference genes in dead bodies than other kinds of RNAs. Based on their tissue-specific expression, we obtained reference genes for three kinds of tissues: miR-122, miR-133a and 18S in heart tissues; LC-Ogdh, circ-AFF1 and miR-122 in liver tissues; and miR-133a, circ-AFF1 in skeletal muscle tissues. For the estimation of post mortem interval (PMI), we also selected suitable biomarkers, which exhibited the best correlation coefficient with PMI. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183042
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http://dx.doi.org/10.1016/j.legalmed.2019.04.002DOI Listing
April 2019
1 Read

The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples.

Leg Med (Tokyo) 2019 Mar 28;38:45-50. Epub 2019 Mar 28.

Department of Forensic Science, College of Criminal Justice, Sam Houston State University, 1003 Bowers Blvd., Huntsville, TX 77340-2525, United States; School of Biomedical Sciences, University of Queensland, St. Lucia, QLD 4072, Australia.

The identification of body fluids in evidentiary stains may provide investigators with probative information during an investigation. In this study, quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays were performed to detect the presence of mRNA and miRNA in fresh and environmentally challenged samples. Blood, semen, and reference markers were chosen for both mRNA/miRNA testing. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223193003
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http://dx.doi.org/10.1016/j.legalmed.2019.03.007DOI Listing
March 2019
4 Reads

Sequence and time course of depletion of cardiac cellular proteins and accumulation of plasma proteins in rat early ischemic myocardium.

Leg Med (Tokyo) 2019 Apr 2;38:36-44. Epub 2019 Apr 2.

Department of Forensic Pathology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.

In order to investigate the sequence and time course of fibronectin (Fn), fibrinogen (Fg), complement (C5), myoglobin (Mb), actin (HHF35), and desmin (Dm) for the diagnosis of early myocardial ischemia, the myocardial ischemia model was established in rats, the positive reaction areas of Fn, Fg and C5 and the depletion areas of Mb, HHF35 and Dm in the ischemic cardiomyocytes were studied with immunohistochemistry, image analysis technique and statistical system. The results showed that the depletion of Dm, HHF35 and Mb, and the positive staining of Fg and C5 in ischemic cardiomyocytes were found as early as 15 min after the myocardial ischemia, but the positive staining of Fn occurred till 3 h after myocardial ischemia. With the prolongation of ischemia, the areas of the depletion of Dm, HHF35, Mb and the positive staining of Fg, C5 and Fn gradually enlarged. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.04.001DOI Listing
April 2019
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An infant autopsy case of bowel obstruction due to internal abdominal hernia.

Leg Med (Tokyo) 2019 Mar 25;38:32-35. Epub 2019 Mar 25.

Department of Forensic Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.

Internal abdominal hernia, defined as protrusion of viscera through a defect of the mesentery, has been considered a rare clinical entity. Recent clinical reviews reported a wide range of onset age (from newborns to the elderly) and symptoms (from minimal abdominal symptoms to severe acute abdomen). Sudden and unexpected death due to internal abdominal hernia is rare in infants or toddlers, and only 4 autopsy cases had been reported previously. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.03.006DOI Listing
March 2019
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Preparation of dentin standard samples for age estimation based on increased aspartic acid racemization rate by heating.

Leg Med (Tokyo) 2019 Mar 18;38:25-31. Epub 2019 Mar 18.

Department of Forensic Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

Age estimation in adults based on aspartic acid racemization (AAR) provides fewer errors and higher precision than that based on bone morphology for the identification of cadavers. The technique has been established in some labs as a routine method. However, as the essential requisites for the technique, a wide age range of teeth of the same type as the target tooth must be collected for calibration for each examination. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.03.005DOI Listing

Dependency relationships among ear characters in a Spanish sample, its forensic interest.

Leg Med (Tokyo) 2019 Mar 18;38:14-24. Epub 2019 Mar 18.

University Institute of Research in Police Sciences (IUICP), Faculty of Law, Alcalá University, Libreros, 27, Ground Floor, 28802-Alcalá de Henares, Madrid, Spain; Department of Physics and Mathematics, Alcalá University, 28871 Alcalá de Henares, Madrid, Spain. Electronic address:

The aim of this paper is to provide information on dependency relationships between the morphological characters of the external ear and their importance in physical identification. At present, there is a lack of population data in this field, and little research has been published. Our study sample consisted of 281 Spanish university students of European descent aged between 18 and 31 years old. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.03.003DOI Listing

Eye temperature measured after death in human bodies as an alternative method of time of death estimation in the early post mortem period. A successive study on new series of cases with exactly known time of death.

Leg Med (Tokyo) 2019 Mar 18;38:10-13. Epub 2019 Mar 18.

Department of Anesthesiology and Intensive Therapy, Medical University of Gdańsk, ul. Smoluchowskiego 17, 80-214 Gdańsk, Poland.

The paper presents a continuation of the studies on time of death (TOD) estimation based on post-mortem temperature measurements in the human eyeball. In the current study, single (in 20 patients) or double (within a 1-hour interval in 10 patients) eyeball and rectal temperature measurements were taken in patients who died in the University Hospital Intensive Therapy Unit. The actual TOD in each patient was exactly known and the body temperature was recorded shortly after (between 50 min and 3 h 30 min). Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.03.004DOI Listing

Accuracy of the third molar index cut-off value for estimating 18 years of age: Validation in a Japanese samples.

Leg Med (Tokyo) 2019 Mar 4;38:5-9. Epub 2019 Mar 4.

AgEstimation Project, Macerata, Italy.

Aims: To evaluate the cut-off value of third molar maturity index (I) = 0.08 for discriminating minors from adults in Japanese population.

Material And Methods: 276 panoramic radiographs of healthy Japanese subjects (139 girls and 137 boys) age range from 14 to 24 years were evaluated. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.03.001DOI Listing
March 2019
1.441 Impact Factor

Sudden unexpected death in a case of necrotizing eosinophilic myocarditis.

Leg Med (Tokyo) 2019 Mar 7;38:1-4. Epub 2019 Mar 7.

DIMEC, University of Bologna, Department of Medical and Surgical Sciences, Section of Legal Medicine, 40126 Bologna, Italy.

Acute myocarditis is related to a significant number of sudden deaths among young adults and its diagnosis is often demanded to post-mortem investigations performed on a forensic setting. Eosinophilic myocarditis (EM) is a rare form of myocarditis that is pathologically characterized by myocardial inflammation with eosinophils, often in association with elevated levels of circulating blood eosinophils. The sudden death of a 19-year-old boy with no past medical history is reported. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.03.002DOI Listing
March 2019
2 Reads

Abnormal detection of Y-STR alleles at DYS385 from female DNA in forensic casework and interchromosomal insertional translocation of P4 palindrome (HSFY/DYS385) from AZFb region.

Leg Med (Tokyo) 2019 Mar 11;37:95-102. Epub 2019 Feb 11.

Seoul Institute, National Forensic Service, 139, Jiyang-ro, Yangcheon-gu, Seoul 08036, Republic of Korea.

The unknown origin of DNA samples derived from crime scenes generates a considerable amount of uncertainty, as do unexpected short tandem repeat (STR) results caused by sample mix-ups, contamination, medical interventions, and transgender individuals (broad meaning). Genetic abnormalities such as somatic/germline mutations, mosaicism or chimerism, sex reversal cases, aneuploidies, and chromosomal structural rearrangements are also possible causes of such results. The evidence offered by the present study suggested that additional DYS385 alleles, as seen in mixed stain samples and in the potentially single-source DNA profile of a female, originated from the female DNA source only. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.02.002DOI Listing
March 2019
1 Read

Current and future directions of high resolution and tandem mass spectrometry in postmortem and human performance toxicology.

Authors:
Michael Fagiola

Leg Med (Tokyo) 2019 Mar 16;37:86-94. Epub 2019 Feb 16.

Nassau County Medical Examiner - Department of Forensic Toxicology, 2251 Hempstead Turnpike - Building R, East Meadow, NY 11554, United States. Electronic address:

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http://dx.doi.org/10.1016/j.legalmed.2019.02.004DOI Listing

Analysis of copy number variation in the NEDD4L gene potentially implicated in body height in the Japanese population.

Leg Med (Tokyo) 2019 Mar 11;37:83-85. Epub 2019 Feb 11.

Department of Medical Genetics and Biochemistry, School of Medical Sciences, University of Fukui, Fukui 910-1193, Japan; Life Science Innovation Center, University of Fukui, Fukui 910-1193, Japan. Electronic address:

Recently it has been recognized that a considerable number of copy number variations (CNVs) are associated with diseases and other complex human traits. In our previous study, we developed a simple quantitative real-time PCR (Q-PCR) method for analysis of CNV copy number, which had the advantage of obviating the need for reference DNA with a known copy number. Using DNA samples obtained from 231 Japanese individuals, we applied this method for analyzing the copy number of a candidate CNV associated with body height, located in the neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L) gene. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.02.003DOI Listing

Histopathology mapping of biochemical changes in diffuse axonal injury by FTIR micro-spectroscopy.

Leg Med (Tokyo) 2019 Mar 2;37:76-82. Epub 2019 Feb 2.

Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China. Electronic address:

The diagnosis of diffuse axonal injury (DAI) is an important task in forensic pathology and clinical medicine. This study aimed to explore the use of Fourier transform infrared spectroscopy (FTIR) to detect DAI. The DAI area of the rat model was detected point by point by the FTIR-mapping system. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.02.001DOI Listing
March 2019
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Worldwide tendency and focused research in forensic anthropology: A bibliometric analysis of decade (2008-2017).

Leg Med (Tokyo) 2019 Mar 22;37:67-75. Epub 2019 Jan 22.

College of Medicine & Forensics, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, PR China; Key Laboratory of the Health Ministry for Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, PR China; Key Laboratory of Shaanxi Province for Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, PR China; State Key Laboratory for Manufacturing Systems Engineering, Xi'an, Shaanxi, PR China. Electronic address:

Purpose: The objective of this study was to illustrate the global research productivity and tendency of forensic anthropology in recent ten years (2008-2017) by bibliometric analysis.

Methods: "Forensic anthropology" was used as the Medical Subject Headings term and topic in PubMed and Web of Science Core Collection.

Results: As 5130 articles retrieved, two independent investigators evaluated all of them respectively. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.01.008DOI Listing
March 2019
3 Reads

Genetic polymorphism of 27 X-chromosomal short tandem repeats in an Egyptian population.

Leg Med (Tokyo) 2019 Mar 24;37:64-66. Epub 2019 Jan 24.

Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

We presented allele frequencies of 27 X-chromosomal short tandem repeats (DXS6807, DXS9902, DXS6795, DXS6810, DXS10076, DXS10077, DXS10078, DXS10162, DXS10163, DXS10164, DXS7132, DXS981, DXS6800, DXS6803, DXS6809, DXS6789, DXS6799, DXS7424, DXS101, DXS7133, GATA172D05, DXS10103, HPRTB, GATA31E08, DXS8377, DXS10147, and DXS7423) obtained from 352 unrelated individuals in Egypt. No deviation from Hardy-Weinberg equilibrium was detected. Two pairs of adjacent loci showed significant linkage disequilibrium. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.01.009DOI Listing
March 2019
2 Reads

Evaluation of the effect of heat damage on DNA extracted from the dental pulp of restored teeth.

Leg Med (Tokyo) 2019 Mar 14;37:54-59. Epub 2019 Jan 14.

Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Ciudad de México 073201, Mexico. Electronic address:

We evaluate structural damage effects of heat on DNA obtained from the dental pulp of restored premolars. We studied three groups (A, B and C) each group comprised twenty premolars extracted from five patients. Three of the four premolars of each donator were restored with different materials: amalgam, glass ionomer and resin, and one unrestored premolar was used as control. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.01.006DOI Listing
March 2019
1.441 Impact Factor

Facial approximation: Soft tissue thickness values for Caucasian males using cone beam computer tomography.

Leg Med (Tokyo) 2019 Mar 4;37:49-53. Epub 2019 Jan 4.

Department of Interdisciplinary Medicine, Section of Legal Medicine, Policlinico di Bari Hospital, University of Bari, Bari, Italy.

The aim of this study was to obtain soft tissue thickness (STTs) of Caucasian males starting from 12 bone landmarks, to allow facial reconstruction and assign an identity to skeletal remains. When a skeleton is found without any circumstantial evidence of identity, through anthropological investigations it is possible to estimate age, sex, and race studying specific characteristics of bones. This first step of identification process helps investigators to narrow down the identity suppositions, but not always forensic analysis guarantees a final positive identification. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.01.002DOI Listing

Genotyping of 49-plex autosomal SNP panel in Iranian Turkmens ethnic group.

Leg Med (Tokyo) 2019 Mar 2;37:45-48. Epub 2019 Jan 2.

Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address:

A total of 94 unrelated individuals from Turkmens ethnic group in Iran were typed for forty-nine of the autosomal single nucleotide polymorphisms (SNPs) in the SNPforID 52plex using the SNaPshot assay. Allele frequencies are presents for the 49 SNPs. No deviation from Hardy-Weinberg equilibrium (HWE) was observed in all but one of the 49 SNP systems and no significant linkage disequilibrium was detected for any SNP pairs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183021
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http://dx.doi.org/10.1016/j.legalmed.2019.01.004DOI Listing
March 2019
4 Reads

Identification of extra alleles in DYS385a/b multi-allelic patterns.

Leg Med (Tokyo) 2019 Mar 2;37:41-44. Epub 2019 Jan 2.

AGCU ScienTech Incorporation, Wuxi 214174, China; School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. Electronic address:

Y chromosome short tandem repeats (Y-STRs) are widely used in forensic DNA analysis. Y-STRs usually genotyped as a monoallelic pattern, or a diallelic pattern at some double-copy loci. Abnormal genotypes with three or four alleles have been reported at DYS385a/b, which is a double-copy locus. Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.01.003DOI Listing
March 2019
4 Reads

Investigation on STR profiling of maternal DNA from a degraded placenta with an abandoned newborn male baby.

Leg Med (Tokyo) 2019 Mar 2;37:37-40. Epub 2019 Jan 2.

Seoul Institute, National Forensic Service, 139, Jiyang-ro, Yangcheon-gu, Seoul 08036, Republic of Korea.

The placenta is a unique and complex organ composed of a mixture of fetal and maternal tissues. In this study, we aimed to detect maternal short tandem repeats (STRs) in degraded placenta from a newborn male baby found abandoned in a river. In order to deduce maternal alleles-which was not possible by sampling of different parts of the placenta-we collected samples from the maternal blood pool in the intervillous space and applied a multi-step method (named tempo-gap DNA extraction) for extracting DNA at defined time points after cell lysis (10 min, 2 h 10 min, and 4 h 10 min). Read More

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http://dx.doi.org/10.1016/j.legalmed.2019.01.001DOI Listing
March 2019
6 Reads

Forensic parameters and mutation analysis of 23 short tandem repeat (PowerPlex® Fusion System) loci in Fujian Han Chinese population.

Leg Med (Tokyo) 2019 Mar 2;37:33-36. Epub 2019 Jan 2.

Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China; Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China. Electronic address:

Kinship testing based on genetic markers has valuable practical applications. Short tandem repeat polymorphisms (STRPs) can have large number of alleles, and become the dominant marker for kinship identification. However, the high mutation rates affect the identification accuracy. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183035
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http://dx.doi.org/10.1016/j.legalmed.2019.01.005DOI Listing
March 2019
9 Reads

Age estimation based on maturation of the medial clavicular epiphysis in a Japanese population using multidetector computed tomography.

Leg Med (Tokyo) 2019 Mar 23;37:28-32. Epub 2018 Dec 23.

Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Education and Research Center of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Electronic address:

To estimate forensic age, the Study Group of Forensic Age Diagnostics of the German Association of Forensic Medicine (AGFAD) has recommended the radiological investigation of the medial clavicular epiphysis (MCE). This study aimed to investigate the relation between the maturation of the MCE and age in a contemporary Japanese population using a standard five-stage system by Schmeling et al. and Kellinghaus substage system. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.12.003DOI Listing

Forensic parameters of 15 autosomal STRs (Identifiler™ kit) in three Mayan groups and one Mestizo population from Guatemala.

Leg Med (Tokyo) 2019 Mar 24;37:25-27. Epub 2018 Dec 24.

Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCiénega-UdeG), Ocotlán, Jalisco, Mexico. Electronic address:

The population of Guatemala includes Mestizos (admixed) and different Mayan groups (Native Americans), which have been poorly studied in regards to short tandem repeat (STR) loci used for human identification (HID) purposes. Therefore, 483 unrelated Guatemalan volunteers from one Mestizo and three Mayan populations (Poqomchi, Ixil, and Achi) were analyzed with an AmpFlSTR Identifiler™ kit. Allele frequencies and forensic parameters were obtained for 15 autosomal STRs in these populations. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.12.004DOI Listing

Age estimation based on the volume change in the maxillary premolar crown using micro CT.

Leg Med (Tokyo) 2019 Mar 15;37:18-24. Epub 2018 Dec 15.

Division of Forensic Odontology, Department of Diagnostic & Therapeutic Science, Meikai University School of Dentistry, Saitama, Japan; Forensic Odontology Center, Meikai University School of Dentistry, Saitama, Japan.

Age is often estimated using teeth because numerous external and internal changes appear due to aging. The purpose of this study was to investigate an age estimation method based on the volume ratio of the internal structure of the crown. 61 maxillary first premolars and 50 maxillary second premolars from subjects aged 20-79 years old were used. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183032
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http://dx.doi.org/10.1016/j.legalmed.2018.12.001DOI Listing
March 2019
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DNA extraction of urinary bladder swabs collected from carbonized and decomposing corpses: Possible application in disaster victim identification.

Leg Med (Tokyo) 2019 Mar 17;37:15-17. Epub 2018 Dec 17.

Post Graduate Studies in Genetics and Molecular Biology (Pós Graduação em Genética e Biologia Molecular), UNIRIO, Rio de Janeiro, Brazil; Forensic Genetics Research Institute (Instituto de Pesquisa e Perícias em Genética Forense), IPPGF/PCERJ, Rio de Janeiro, Brazil.

A disaster is an unexpected event causing death or injury to many people. In such events, a large number of casualties may take place, exposing corpses to a harsh environment for days or months. DNA profiling is recognized as one of the primary methods for identifying mass disaster victims, especially when it involves decomposed or fragmented bodies. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183023
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http://dx.doi.org/10.1016/j.legalmed.2018.12.002DOI Listing
March 2019
1 Read

Cellular uptake of paraquat determines subsequent toxicity including mitochondrial damage in lung epithelial cells.

Leg Med (Tokyo) 2019 Mar 22;37:7-14. Epub 2018 Nov 22.

Department of Forensic Medicine, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.

Paraquat (PQ) is one of the commonly used herbicides in the world, despite its high toxicity. The ingestion of PQ accidentally or intentionally causes severe damage in diverse organs including the lung. Pulmonary fibrosis triggered by PQ accumulation in the lung epithelial cells is one of the major causes of death. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183031
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http://dx.doi.org/10.1016/j.legalmed.2018.11.008DOI Listing
March 2019
2 Reads

Detection of cocaine and metabolites from mouse femur buried in soil.

Leg Med (Tokyo) 2019 Mar 9;37:1-6. Epub 2018 Nov 9.

Department of Legal Medicine, School of Medicine, Tokyo Women's Medical University, Tokyo 162-8666, Japan.

Drug addicts are frequently involved in committing homicides, and burial in the ground is often performed by offenders for body disposal. Therefore, toxicological analyses of buried bones are vitally important for investigating the cause and circumstance of death. Cocaine concentrations in heart blood, femoral muscle, and femur were measured in mice after injections of 5, 15, and 30 mg/kg cocaine and in femurs buried in soil. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.11.005DOI Listing
March 2019
10 Reads

Genetic diversity and haplotype structure of 27 Y-STR loci in a Yanbian Korean population from Jilin Province, Northeast China.

Leg Med (Tokyo) 2019 Feb 22;36:110-112. Epub 2018 Nov 22.

Institute of Forensic Medicine and Laboratory Medicine, Jining Medical University, Forensic Science Center of Jining Medical University, Jining, Shandong 272067, PR China. Electronic address:

In this study, 27 Y-STRs were analyzed in 347 male individuals from the Yanbian Korean population. Haplotype diversity (HD) and discrimination capacity (DC) values were calculated. Pairwise R values were evaluated in AMOVA analysis and visualized through multidimensional scaling (MDS). Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.11.010DOI Listing
February 2019

Collagen degradation as a possibility to determine the post-mortem interval (PMI) of human bones in a forensic context - A survey.

Leg Med (Tokyo) 2019 Feb 22;36:96-102. Epub 2018 Nov 22.

Institute of Biologic Anthropology, Albert-Ludwigs-University, Hebelstraße 29, 79104 Freiburg im Breisgau, Germany.

To find out the time since death (PMI) of a human individual, from which only skeletal remains are left is a difficult question to answer, since methods currently available don't bring concrete answers. Subsequent to our validation study on porcine bones, we focused on human bones analysing if collagen degradation would occur at a predictive rate so that the method may be used for PMI-estimation. We tried to reproduce a method for determining the Co/NCo (Collagen to Non-Collagen) ratio of porcine bones Boaks et al. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183029
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http://dx.doi.org/10.1016/j.legalmed.2018.11.009DOI Listing
February 2019
6 Reads

Use of a proforma to aid in reducing coercion into informal admission for acute adult psychiatric inpatients in the U.K.

Leg Med (Tokyo) 2019 Feb 23;36:103-109. Epub 2018 Nov 23.

Coventry and Warwickshire Partnership NHS Trust, Caludon Centre, Clifford Bridge Road, Coventry, England CV2 2TE, United Kingdom.

Background: People with acute psychiatric illness may be at risk of coercion into informal admission. A lack of capacity assessment (CA) and provision of adequate information (PAI) for informal patients may constitute a risk of coercive admitting practice, resulting in increased use of the mental health act (MHA) in the days following admission. We developed and tested a proforma to aid in ensuring CA and PAI for informal admissions. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.11.011DOI Listing
February 2019

The epidemiology of sexual assault of older female nursing home residents, in Victoria Australia, between 2000 and 2015.

Leg Med (Tokyo) 2019 Feb 10;36:89-95. Epub 2018 Nov 10.

Department of Forensic Medicine, Monash University, Southbank, Victoria 3006, Australia.

Sexual assault is the least acknowledged, detected, and reported type of assault against nursing home residents. Nursing home staff are responsible for reporting suspected allegations to the police, who will contact a clinical forensic examiner to conduct a forensic medical examination. This study examined the epidemiology of sexual assaults of older women (aged 65 years and older) residing in nursing homes in Victoria, Australia, between 2000 and 2015, whose alleged incidents were referred to a clinical forensic examiner for a forensic medical examination. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.11.006DOI Listing
February 2019
10 Reads

Extensive gastric necrosis secondary to acute gastric dilatation: A case report.

Leg Med (Tokyo) 2019 Feb 13;36:85-88. Epub 2018 Nov 13.

Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan.

We report a case of sudden death in a patient who developed extensive gastric necrosis secondary to acute gastric dilatation. A 36-year-old man with mental retardation (but without difficulties in activities of daily living), developed an illness after a meal out with friends, necessitating 3 hospital visits. He returned home after receiving drug therapy; however, his condition deteriorated, and he was transferred to our hospital via ambulance. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183032
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http://dx.doi.org/10.1016/j.legalmed.2018.11.007DOI Listing
February 2019
15 Reads

Fatal accidental asphyxia in the reverse jack-knife position on a chair with wheels.

Leg Med (Tokyo) 2019 02 3;36:81-84. Epub 2018 Nov 3.

Department of Forensic Medicine, Graduate School of Biomedical Sciences, Tokushima University, Japan.

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http://dx.doi.org/10.1016/j.legalmed.2018.11.001DOI Listing
February 2019

Forensic microbiology applications: A systematic review.

Leg Med (Tokyo) 2019 Feb 3;36:73-80. Epub 2018 Nov 3.

Legal Medicine Section - Department for Health Promotion and Mother-Child Care, University of Palermo, Via del Vespro, 129, 90127, Italy. Electronic address:

According to the Human Microbiome Project (HMP), a healthy human body contains ten times more microbes than human cells. Microbial communities colonize different organs of the body, playing fundamental roles both in human health and disease. Despite the vast scientific knowledge of the role of microbial communities in a living body, little is known at present about microbial changes occurring after death, thus leading many authors to investigate the composition of the thanatomicrobiome and its potential applications in the forensic field. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.11.002DOI Listing
February 2019
19 Reads

Na/K-ATPase, acetylcholinesterase and glutathione S-transferase activities as new markers of postmortem interval in Swiss mice.

Leg Med (Tokyo) 2019 Feb 3;36:67-72. Epub 2018 Nov 3.

Programa de Pós-graduação em Bioquímica e Bioprospecção, Laboratório de Pesquisa em Farmacologia Bioquímica (LaFarBio), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), CEP 96010-900 Pelotas, RS, Brazil. Electronic address:

Determining precisely the postmortem interval (PMI) is a key parameter for forensic researches, given that various physical, biochemical and metabolic changes begin to occur in the body after death. In the present study, the Na/K-ATPase, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated. For this, male adult Swiss mice were killed by isoflurane inhalation anesthesia and divided into four groups according to time of death (0, 6, 24 and 48 h). Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.11.003DOI Listing
February 2019
15 Reads

Quantification of secondary dentin formation based on the analysis of MDCT scans and dental OPGs in a contemporary Malaysian population.

Leg Med (Tokyo) 2019 02 3;36:59-66. Epub 2018 Nov 3.

Centre for Forensic Anthropology, M420, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.

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http://dx.doi.org/10.1016/j.legalmed.2018.11.004DOI Listing
February 2019
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Dental age estimation in Malaysian adults based on volumetric analysis of pulp/tooth ratio using CBCT data.

Leg Med (Tokyo) 2019 Feb 21;36:50-58. Epub 2018 Oct 21.

Department of Dental Research Management Centre, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia. Electronic address:

Forensic odontology plays an important role in human identification and dental age estimation is an integral part of this process. The aim of the study was to investigate the association between chronological age and pulp/tooth volume ratio in a Malaysian population (Malays and Chinese) from cone-beam computed tomography (CBCT) scans, enhanced with Mimics software. Three hundred CBCT scans of 153 males and 147 females, aged between 16 and 65 years were divided into 5 age groups. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.10.005DOI Listing
February 2019
19 Reads

Pathology diagnosis of an atypical thyroid cartilage lesion.

Leg Med (Tokyo) 2019 Feb 19;36:47-49. Epub 2018 Oct 19.

CHU Poitiers, Département de médecine légale, IML/UMJ, Poitiers, France; Univ Poitiers, Faculté de médecine et de pharmacie, Poitiers, France.

In forensic practice, laryngeal lesions are of particular interest for the pathologist, as they may sign foul play. We report the case of a decedent, whose body was found in an advanced state of putrefaction, at the bottom of a ledge, after a one-month disappearance. The face and neck were skeletised. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.10.003DOI Listing
February 2019
11 Reads

Undetected severe complication after elective lumbar surgery - How could it happen?

Leg Med (Tokyo) 2019 02 19;36:43-46. Epub 2018 Oct 19.

Department of Legal Medicine, Ludwig Maximilians University, Munich, Germany.

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http://dx.doi.org/10.1016/j.legalmed.2018.10.004DOI Listing
February 2019

Genetic polymorphisms of 20 STR loci in a Chaoshan group from Jieyang, China.

Leg Med (Tokyo) 2019 Feb 1;36:41-42. Epub 2018 Oct 1.

School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. Electronic address:

We obtained allelic frequencies and forensic parameters of 20 short tandem repeat (STR) loci (D3S1358, FGA, D5S818, D7S820, CSF1PO, D16S539, D19S433, vWA, D8S1179, D18S51, D13S317, TPOX, TH01, D2S1338, D12S391, D1S1656, D21S11, D6S1043, Penta D, Penta E) from 529 unrelated individuals in Jieyang Han population using PowerPlex® 21 (Promega, Madison, Wi, USA). The relationship between the Jieyang Han group and other Han populations was studied and the results showed that the Jieyang Han population had the closest genetic relationship with the Fujian Han population. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.09.016DOI Listing
February 2019
6 Reads
1.440 Impact Factor

Autopsy case of right ventricular rhabdomyoma in tuberous sclerosis complex.

Leg Med (Tokyo) 2019 Feb 9;36:37-40. Epub 2018 Oct 9.

Medical Examiner's Office of Hyogo Prefecture, Kobe, Japan; Department of Forensic Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.

Tuberous sclerosis complex (TSC) is a genetic multisystem disorder characterized by widespread hamartomas in several organs, including the brain, heart, skin, eyes, kidney, lung, and liver. Rhabdomyoma is the most common cardiac tumor diagnosed in fetuses, neonates and infants, and is closely linked to TSC. Here we describe an autopsy case of right ventricular rhabdomyoma in TSC. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.10.001DOI Listing
February 2019
14 Reads

An experimental study on investigating the postmortem interval in dichlorvos poisoned rats by GC/MS-based metabolomics.

Leg Med (Tokyo) 2019 Feb 11;36:28-36. Epub 2018 Oct 11.

West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. Electronic address:

The estimation of the postmortem interval (PMI) is always a key issue in forensic science. Although many attempts based on metabolomics approaches have been proven to be feasible and accurate for PMI estimation, there have been no reports regarding the determination of the PMI in acute dichlorvos (DDVP) poisoning. In this study, all rats were killed by acute DDVP poisoning at a dose three fold the oral LD (240 mg/kg). Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.10.002DOI Listing
February 2019

Traumatic axonal injury revealed by postmortem magnetic resonance imaging: A case report.

Leg Med (Tokyo) 2019 Feb 1;36:9-16. Epub 2018 Oct 1.

Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.

In forensic investigations, it is important to detect traumatic axonal injuries (TAIs) to reveal head trauma that might otherwise remain occult. These lesions are subtle and frequently ambiguous on macroscopic evaluations. We present a case of TAI revealed by pre-autopsy postmortem magnetic resonance imaging (PMMR). Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.09.019DOI Listing
February 2019
6 Reads

Association between sudden unexpected deaths in bathtubs and ambient temperature among elderly Japanese adults: A time-series regression study.

Leg Med (Tokyo) 2019 Feb 3;36:21-27. Epub 2018 Oct 3.

Department of Legal Medicine, Nippon Medical School, 1-1-5 Sendagi Bunkyo-ku, Tokyo 113-8602, Japan; Tokyo Medical Examiner's Office, Tokyo Metropolitan Government, Bunkyo-ku, Tokyo, Japan. Electronic address:

Background: Sudden unexpected deaths in bathtubs among elderly Japanese adults occur predominantly during the cold season. This study investigated the relationship between these deaths and bathing day temperature among elderly adults in Tokyo.

Methods: Data for 1408 cases of bath-related deaths from January 1 to December 31, 2015 were obtained from the Tokyo Medical Examiner's Office. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.09.021DOI Listing
February 2019
7 Reads

Association of SNPs in transferrin and transferrin receptor genes with blood iron levels in human.

Leg Med (Tokyo) 2019 Feb 4;36:17-20. Epub 2018 Oct 4.

Department of Legal Medicine, Shimane University School of Medicine, Izumo 693-8501, Japan.

Iron is bound to mobile transferrin (TF) and ferritin in blood. TF receptors (TFRC and TFR2) regulate intracellular iron by delivering iron from TF into the cytoplasm. In this study, we examined the effects of 10 single nucleotide polymorphisms (SNPs) in each of the genes for TF and TF receptors on blood iron concentrations in Japanese subjects. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.09.022DOI Listing
February 2019
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A quantitative morphological analysis of three-dimensional CT coxal bone images of contemporary Japanese using homologous models for sex and age estimation.

Leg Med (Tokyo) 2019 Feb 1;36:1-8. Epub 2018 Oct 1.

Division of Forensic Medicine, Department of Forensic Science, Iwate Medical University, Iwate 028-3694, Japan.

Sexual dimorphisms and age-dependent morphological features of the human coxal bone were quantitatively analyzed using homologous models created from three-dimensional (3D) computed tomography images of the pelvis (male: 514 samples, female: 388 samples, age 16-100). Bilateral average coxal images of each sex and age decade were generated separately through principle component analyses (PCA). By measuring average point-to-point distances of 8472 corresponding points (average corresponding point differences [ACPDs]) between each homologous coxal image and the average images, the sex of more than 93% of the samples was correctly assigned. Read More

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http://dx.doi.org/10.1016/j.legalmed.2018.09.017DOI Listing
February 2019
12 Reads

The evaluation of Oryza sativa L (Black rice) extracts for detection of spermatozoa on the clothing and vaginal swab samples.

Leg Med (Tokyo) 2018 Nov 2;35:91-97. Epub 2018 Oct 2.

Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Thailand; Department of Forensic Sciences, Faculty of Allied Health Sciences, Thammasat University, Thailand. Electronic address:

Investigation of sexual assault cases from the evidence involving vaginal swab, clothing and others is examined by a forensic scientist. The explanation of trace findings on spermatozoa on clothing is often problematic due to the use of different staining methods. Conventional staining method used either Papanicolaou (PAP) or Dip quick® stain as synthetic dyes which are expensive imported material and harmful to human health. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13446223183023
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http://dx.doi.org/10.1016/j.legalmed.2018.09.020DOI Listing
November 2018
1 Read