24,983 results match your criteria Lactose Intolerance


Lactose in processed foods: evaluating the availability of information regarding its amount.

Cien Saude Colet 2018 Dec;23(12):4119-4128

Curso de Nutrição e Metabolismo, Departamento de Ciências da Saúde, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo. Ribeirão Preto SP Brasil.

Patients with lactose intolerance, a high prevalence disease, should control the intake of food with lactose to avoid gastrointestinal symptoms. Since tolerance depends on the amount of lactose ingested, it is essential to discuss the availability of the amount of lactose information in processed foods, which is currently not mandatory. The study measured the availability of information on the amount of lactose on the labels of processed food that contained milk or lactose in the ingredient list. Read More

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December 2018
1 Read

Expression, purification, and characterization of pyruvate kinase from : A key allosteric regulatory enzyme.

Int J Mycobacteriol 2018 Oct-Dec;7(4):368-374

Department of Biotechnology, Kuvempu University, Shimoga, Karnataka, India.

Background: The current research aims to isolate pyruvate kinase (Pyk) gene from Mycobacterium tuberculosis and expression of the gene (Rv1617) to obtain a purified enzyme. The enzyme activity and secondary structural features were assessed through biochemical assays and circular dichroism (CD) spectroscopy, respectively.

Methods: The Pyk-encoding gene from the complete genome of M. Read More

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December 2018

Function Perturbation Impact on Feedback Stabilization of Boolean Control Networks.

IEEE Trans Neural Netw Learn Syst 2018 Dec 5. Epub 2018 Dec 5.

Function perturbation analysis of Boolean networks is an important topic in the study of gene regulation due to gene mutation or immeasurable variables. This brief studies the function perturbation impact on feedback stabilization of Boolean control networks (BCNs) by using the algebraic state space representation approach. First, the state feedback stabilization control design of BCNs is recalled and the function perturbation problem is formulated. Read More

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December 2018

Screening novel β-galactosidases from a sequence-based metagenome and characterization of an alkaline β-galactosidase for the enzymatic synthesis of galactooligosaccharides.

Protein Expr Purif 2018 Dec 4;155:104-111. Epub 2018 Dec 4.

State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

βgalactosidases have wide industrial applications in lactose hydrolysis and transglycosylation reactions. Therefore, there is a need to mine novel and high-quality β-galactosidases with good tolerance and novel features from harsh environments and genomic databases. In this study, an Escherichia coli β-galactosidase-deficient host, ΔlacZ(DE3)pRARE, was constructed by the CRISPR-Cas9 system for screening active β-galactosidases. Read More

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December 2018
1.695 Impact Factor

Energy and nitrogen partitioning in dairy cows at low or high metabolizable protein levels is affected differently by postrumen glucogenic and lipogenic substrates.

J Dairy Sci 2018 Oct 24. Epub 2018 Oct 24.

Wageningen Livestock Research, Wageningen University and Research, PO Box 338, 6700 AH Wageningen, the Netherlands.

This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle. Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest. A total mixed ration consisting of 42% corn silage, 31% grass silage, and 27% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and metabolizable protein requirements, respectively, and was fed at 90% of ad libitum intake by individual cow. Read More

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October 2018

Effects of timing of palmitic acid supplementation on production responses of early-lactation dairy cows.

Authors:
J de Souza A L Lock

J Dairy Sci 2018 Oct 24. Epub 2018 Oct 24.

Department of Animal Science, Michigan State University, East Lansing 48824. Electronic address:

The objective of our study was to evaluate the effects of timing of palmitic acid (C16:0) supplementation on production responses of early-lactation dairy cows. Fifty-two multiparous cows were used in a randomized complete block design experiment. During the fresh period (FR; 1-24 d in milk) cows were assigned to either a control diet containing no supplemental fat (CON) or a diet supplemented with C16:0 (palmitic acid, PA; 1. Read More

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October 2018

Photoinactivation of the Staphylococcus aureus Lactose-Specific EIICB Phosphotransferase Component with p-azidophenyl-β-D-Galactoside and Phosphorylation of the Covalently Bound Substrate.

J Mol Microbiol Biotechnol 2018 Dec 6;28(3):147-158. Epub 2018 Dec 6.

Max Planck Institute for Systems Physiology, Dortmund,

Background: The phosphoenolpyruvate (PEP):lactose phosphotransferase system of Staphylococcus aureus transports and phosphorylates lactose and various phenylgalactosides. Their phosphorylation is catalyzed by the Cys476-phosphorylated EIIB domain of the lactose-specific permease enzyme IICB (EIICBLac). Phosphorylation causes the release of galactosides bound to the EIIC domain into the cytoplasm by a mechanism not yet understood. Read More

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December 2018
4 Reads

Janus glycosides of next generation: Synthesis of 4-(3-chloropropoxy)phenyl and 4-(3-azidopropoxy)phenyl glycosides.

Carbohydr Res 2018 Nov 25;471:95-104. Epub 2018 Nov 25.

N. D. Zelinsky Institute of Organic Chemistry of the Russian Academy of Sciences, Leninsky prosp., 47, 119991, Moscow, Russian Federation. Electronic address:

Efficient procedures for the preparative synthesis of per-O-acyl derivatives of 4-(3-chloropropoxy)phenyl (CPP) glycosides of a series of common mono- and disaccharides (d-glucose, d-galactose, d-mannose, l-rhamnose, d-arabinofuranose, d-glucosamine, lactose) are described. The CPP glycosides obtained were transformed in almost quantitative yields to the corresponding unprotected 4-(3-azidopropoxy)phenyl (APP) glycosides, which could become next-generation Janus glycosides with cleavable spacer aglycon, ready for conjugation or further transformation. Read More

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November 2018
1.929 Impact Factor

On-chip biosynthesis of GM1 pentasaccharide-related complex glycans.

Chem Commun (Camb) 2018 Dec 3. Epub 2018 Dec 3.

School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 37673, Korea and Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Korea.

A functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and to apply glycan-involved applications simultaneously. GM3 trisaccharide, GM2 tetrasaccharide, and GM1 pentasaccharide were successfully directly biosynthesized on lactose-immobilized surfaces through three consecutive glycosyltransferase reactions along with small amounts of enzymes and donors, without any additional processes. Biosynthesized GM1 pentasaccharide-related complex glycans were demonstrated to provide information on the substrate specificity of whole cholera toxin. Read More

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December 2018
1 Read

Clinical Efficacy Of Lactase Enzyme Supplement In Infant Colic: A Randomised Controlled Trial.

J Pak Med Assoc 2018 Dec;68(12):1744-1747

Kharadar General Hospital, Karachi.

Objective: To determine the efficacy of lactase enzyme supplement in infant colic.

Methods: The double-blind randomised clinical trial was conducted from November 2014 to June 2017 at Kharadar General Hospital, Karachi, and comprised infants aged 0-6 months with infant colic, excessive crying lasting at least 3 hours a day on at least 3 days a week for at least 3 weeks. The subjects were randomised into intervention group A which received lactase enzyme Colibid, and placebo group B. Read More

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December 2018

[Optimization of Nasal Drug Absorption from Powder Formulations: The Feasibility of Controlling Drug Absorption by the Use of Pharmaceutical Excipients].

Authors:
Akiko Tanaka

Yakugaku Zasshi 2018 ;138(12):1467-1472

Department of Biopharmaceutics, Kyoto Pharmaceutical University.

Nasal application of powder formulations has garnered attention because of its significant potential for systemic drug delivery. Because a powder drug must first diffuse from the formulation and dissolve in the nasal cavity fluid before transepithelial permeation, dissolution and diffusion are distinct but important factors for nasal drug absorption. Since the formulation is directly administered onto the nasal mucosal surface, the effect of excipients on drug absorption may be significant. Read More

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January 2018

The Role of Disaccharidase Deficiencies in Functional Abdominal Pain Disorders-A Narrative Review.

Nutrients 2018 Nov 29;10(12). Epub 2018 Nov 29.

Department of Pediatric Gastroenterology, Hepatology and Nutrition, University of Miami Miller School of Medicine, 1601 NW 12th Ave, Miami, FL 33137, USA.

Disaccharidase deficiencies are reportedly underdiagnosed in pediatric populations. Though typically thought to cause diarrheal disease, they can also be a cause of abdominal pain and dyspepsia, and patients diagnosed with these functional disorders may actually have associated enzyme deficiencies. While the effects of lactose deficiency have been widely studied, sucrase, maltase, and isomaltase are less frequently considered when approaching a patient with an apparent functional abdominal pain disorder. Read More

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November 2018

Comparative effectiveness and safety of interventions for acute diarrhea and gastroenteritis in children: A systematic review and network meta-analysis.

PLoS One 2018 5;13(12):e0207701. Epub 2018 Dec 5.

Department of Health Research Methods, Evidence & Impact; McMaster University, Hamilton, Canada.

Background: Many interventions have shown effectiveness in reducing the duration of acute diarrhea and gastroenteritis (ADG) in children. Yet, there is lack of comparative efficacy of interventions that seem to be better than placebo among which, the clinicians must choose. Our aim was to determine the comparative effectiveness and safety of the pharmacological and nutritional interventions for reducing the duration of ADG in children. Read More

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December 2018
1 Read
3.234 Impact Factor

Cereal type significantly affects the composition and reconstitution characteristics of dried fermented milk-cereal composites.

J Sci Food Agric 2018 Dec 5. Epub 2018 Dec 5.

Teagasc Food Research Centre Moorepark, Fermoy, Co. Cork, Ireland.

Background: Dairy and cereal are frequently combined to create composites with enhanced nutritional benefits. Commercially-available dried dairy-cereal composites are typically reconstituted and cooked to produce porridge or soup.

Results: Dried fermented milk-cereal composites (FMCC) with ~ 193 g kg protein were prepared by blending fermented milk with parboiled oats (FMCCo), wheat (FMCCw) or barley (FMCCb), incubating the blend, drying and milling. Read More

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December 2018
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Biological Activities of Lactose-Based Prebiotics and Symbiosis with Probiotics on Controlling Osteoporosis, Blood-Lipid and Glucose Levels.

Medicina (Kaunas) 2018 Dec 3;54(6). Epub 2018 Dec 3.

Department of Food Engineering, Faculty of Food Science, Szent István University, Ménesi st 44, HU-1118 Budapest, Hungary.

Lactose-based prebiotics are synthesized by enzymatic- or microbial- biotransformation of lactose and have unique functional values. In this comprehensive review article, the biochemical mechanisms of controlling osteoporosis, blood-lipid, and glucose levels by lactose-based prebiotics and symbiosis with probiotics are reported along with the results of clinical investigations. Interaction between lactose-based prebiotics and probiotics reduces osteoporosis by (a) transforming insoluble inorganic salts to soluble and increasing their absorption to gut wall; (b) maintaining and protecting mineral absorption surface in the intestine; (c) increasing the expression of calcium-binding proteins in the gut wall; (d) remodeling osteoclasts and osteoblasts formation; (e) releasing bone modulating factors; and (f) degrading mineral complexing phytic acid. Read More

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December 2018

Determination of chemical stability of sitagliptin by LC-UV, LC-MS and FT-IR methods.

J Pharm Biomed Anal 2018 Nov 15;164:789-807. Epub 2018 Nov 15.

Department of Medicinal Chemistry, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland.

Sitagliptin was stored at high temperature/high humidity, dry hot air, UV/VIS light and different pH. Then, a selective LC-UV method was developed for determination of sitagliptin in the presence of degradation products and for estimation of degradation kinetics. Because parent drugs can react with excipients in final pharmaceutical formulations, stability of sitagliptin was also examined in the presence of excipients of different reactivity, using FT-IR and LC-UV methods. Read More

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November 2018

Cheese Yeasts.

Yeast 2018 Dec 4. Epub 2018 Dec 4.

Agroscope, Schwarzenburgstrasse 161, CH-3003, Bern, Switzerland.

Numerous traditionally aged cheeses are surface ripened and develop a biofilm, known as the cheese rind, on their surfaces. The rind of such cheeses comprises a complex community of bacterial and fungal species that are jointly responsible for the typical characteristics of the various cheese varieties. Surface ripening starts directly after brining with the rapid colonization of the cheese surface by yeasts. Read More

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December 2018

Nonfat milk attenuates acute hyperglycemia in individuals with android obesity: A randomized control trial.

Food Sci Nutr 2018 Nov 12;6(8):2104-2112. Epub 2018 Sep 12.

Cardiovascular Aging Research Laboratory Department of Kinesiology and Health Education The University of Texas at Austin Austin Texas.

Background: Elevated android body fat increases the risk of developing cardiometabolic diseases. Postprandial hyperglycemia contributes to the proatherogenic metabolic state evident in android adiposity. Due to the insulinotropic effect of milk-derived proteins, postprandial hyperglycemia has been shown to be reduced with the addition of dairy products. Read More

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November 2018

Interpreting rapid diagnostic test (RDT) for Plasmodium falciparum.

BMC Res Notes 2018 Dec 4;11(1):850. Epub 2018 Dec 4.

Department of Medical Microbiology and Parasitology, College of Medicine of the University of Lagos, Idi-araba, Lagos, Nigeria.

Objective: Rapid diagnostic tests have been of tremendous help in malaria control in endemic areas, helping in diagnosis and treatment of malaria cases. It is heavily relied upon in many endemic areas where microscopy cannot be obtained. However, caution should be taken in the interpretation of its result in clinical setting due to its limitations and inherent weakness. Read More

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December 2018
1 Read

Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats.

Br J Nutr 2018 Dec;120(12):1338-1348

2Departamento de Fisiologia,Disciplina de Fisiologia da Nutrição,Universidade Federal de São Paulo,Rua Botucatu 862,2° andar,04023-060 São Paulo,SP, Brazil.

Studies have shown the positive effects of prebiotics on the intestinal absorption of Ca and Fe. The present study evaluated the effect of fructo-oligosaccharide (FOS) supplementation in soya beverage (SB) on absorption mechanisms of Ca and Fe in recently weaned rats. Male Wistar rats were divided into four groups: lactose-free cows' milk (CM), lactose-free CM with FOS (0·8 g/100 ml) (CMF), SB and soya beverage with FOS (0·8 g/100 ml) (SBF). Read More

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December 2018
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Optimization of the 503 antigen induction strategy of Leishmania infantum chagasi expressed in Escherichia coli M15.

Prep Biochem Biotechnol 2018 Nov 29:1-9. Epub 2018 Nov 29.

a Biochemical Engineering Laboratory Chemical Engineering Postgraduate Studies Department , Federal University of Rio Grande do Norte , Natal , Brazil.

Leishmaniosis is a complex of diseases that can be fatal, if not given proper attention. Despite its relevance in the public health system, there is no vaccine capable of preventing the disease in humans so far and its treatment is expensive and aggressive to human health. The present study aims to optimize the induction parameters of the 503 Leishmania i. Read More

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November 2018

Cloning, Expression and Characterization of a Novel Cold-adapted β-galactosidase from the Deep-sea Bacterium sp. ML52.

Mar Drugs 2018 Nov 27;16(12). Epub 2018 Nov 27.

Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

The bacterium sp. ML52, isolated from deep-sea water, was found to synthesize an intracellular cold-adapted β-galactosidase. A novel β-galactosidase gene from strain ML52, encoding 1058 amino acids residues, was cloned and expressed in . Read More

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November 2018
2.853 Impact Factor

Longitudinal study of breastfeeding and growth in 0-6 month infants.

Asia Pac J Clin Nutr 2018 ;27(6):1294-1301

Department of Early Child Development, Capital Institute of Pediatrics, Beijing, China.

Background And Objectives: To explore advantages and challenges for exclusive breastfeeding (EBF), compared to non-exclusive breastfeeding (nEBF).

Methods And Study Design: Mothers from 7 cities in China were visited at 3, 10, 60, 120, and 180 days postpartum. Data about feeding practices, infant growth, and the macronutrient contents of human milk (HM) were collected. Read More

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January 2018
7 Reads

Enhanced Properties and Lactose Hydrolysis Efficiencies of Food-Grade β-Galactosidases Immobilized on Various Supports: a Comparative Approach.

Appl Biochem Biotechnol 2018 Nov 28. Epub 2018 Nov 28.

College of Food and Biological Engineering, Qiqihar University, Qiqihar, 161006, People's Republic of China.

In this study, a fungal and two yeast β-galactosidases were immobilized using alginate and chitosan. The biochemical parameters and lactose hydrolysis abilities of immobilized enzymes were analyzed. The pH optima of immobilized fungal β-galactosidases shifted to more acidic pH compared to free enzyme. Read More

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November 2018

Low FODMAP diet in Egyptian patients with Crohn's disease in remission phase with functional gastrointestinal symptoms.

JGH Open 2018 Feb 5;2(1):15-20. Epub 2018 Feb 5.

Gastroenterology Unit, Faculty of Medicine Alexandria University Alexandria Egypt.

Background And Aim: Despite being in remission, functional gastrointestinal disease (FGID) in Crohn's disease (CD) patients can reduce their quality of life. The Egyptian daily diet contains a high amount of FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, And Polyols). As the low FODMAP diet has been proven to be effective in irritable bowel syndrome worldwide, it was reasonable to take a step further and begin to study the effect of low FODMAP in Egyptian CD patients with FGID. Read More

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February 2018
1 Read

Engineered occluded apo-intermediate of LacY.

Proc Natl Acad Sci U S A 2018 Dec 26;115(50):12716-12721. Epub 2018 Nov 26.

Department of Physiology, University of California, Los Angeles, CA 90095-7327;

The lactose permease of (LacY) utilizes an alternating access symport mechanism with multiple conformational intermediates, but only inward (cytoplasmic)- or outward (periplasmic)-open structures have been characterized by X-ray crystallography. It is demonstrated here with sugar-binding studies that cross-linking paired-Cys replacements across the closed cytoplasmic cavity stabilize an occluded conformer with an inaccessible sugar-binding site. In addition, a nanobody (Nb) that stabilizes a periplasmic-open conformer with an easily accessible sugar-binding site in WT LacY fails to cause the cytoplasmic cross-linked mutants to become accessible to galactoside, showing that the periplasmic cavity is closed. Read More

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December 2018
1 Read

GC-MS metabolite profiling for specific detection of dwarf somaclonal variation in banana plants.

Appl Plant Sci 2018 Nov 12;6(11):e01194. Epub 2018 Nov 12.

Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador.

Premise Of The Study: The production of banana ( spp.; Musaceae) plants is affected by various types of somaclonal variations (SV), including dwarfism. However, methods for specific detection of SV are still scarce. Read More

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November 2018
1 Read

The putative β-glucosidase BGL3I regulates cellulase induction in .

Biotechnol Biofuels 2018 19;11:314. Epub 2018 Nov 19.

1CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai, 200032 China.

Background: The filamentous fungus (anamorph of ) displays increased cellulase expression while growing on inducers such as lactose or cellulose. However, the mechanism of cellulase induction in is not yet completely characterized. Here, a protein annotated as β-glucosidase (BGL3I) was found to be involved in cellulase induction in . Read More

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November 2018

Bioconversion of cheese whey permeate into fungal oil by .

J Biol Eng 2018 14;12:25. Epub 2018 Nov 14.

1Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616 USA.

Background: Oleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus was cultivated in whey permeate, a byproduct from cheese production, to produce an oil-rich fungal biomass. Response surface methodology was used to optimize the fermentation conditions such as pH and temperature for increased biomass yield and lipid accumulation. Read More

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November 2018

Role of Temperate Bacteriophage ϕ20617 on DSM 20617 Autolysis and Biology.

Front Microbiol 2018 9;9:2719. Epub 2018 Nov 9.

Department of Food Environmental and Nutritional Sciences, University of Milan, Milan, Italy.

DSM 20167 showed autolytic behavior when cultured in lactose- and sucrose-limited conditions. The amount of cell lysis induced was inversely related to the energetic status of the cells, as demonstrated by exposing cells to membrane-uncoupling and glycolysis inhibitors. Genome sequence analysis of strain DSM 20617 revealed the presence of a -type temperate bacteriophage, designated Φ20617, whose genomic organization and structure resemble those of temperate streptococcal bacteriophages. Read More

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November 2018
5 Reads

Contribution of the inflammasome to inflammaging.

J Inflamm (Lond) 2018 16;15:23. Epub 2018 Nov 16.

Department of Neurological Surgery, Lois Pope LIFE Center, The Miami Project to Cure Paralysis, Miller School of Medicine, University of Miami, 1095 NW 14th Terrace, 3-25, Miami, FL 33136-1060 USA.

Background: Inflammation is a natural part of the aging process. This process is referred to as inflammaging. Inflammaging has been associated with deleterious outcomes in the aging brain in diseases such as Alzheimer's disease and Parkinson's disease. Read More

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November 2018
1 Read

Colloidal properties of protein complexes formed in β-casein concentrate solutions as influenced by heating and cooling in the presence of different solutes.

Colloids Surf B Biointerfaces 2018 Oct 28;174:343-351. Epub 2018 Oct 28.

Department of Food Science, University of Wisconsin-Madison, WI, USA; Center for Dairy Research, University of Wisconsin-Madison, WI, USA.

Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature. The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration. The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN. Read More

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October 2018
6 Reads

Hypertonic milk replacers increase gastrointestinal permeability in healthy dairy calves.

J Dairy Sci 2018 Nov 21. Epub 2018 Nov 21.

Trouw Nutrition Research and Development, PO Box 299, 3800 AG, Amersfoort, the Netherlands.

Hypertonic milk replacers are commonly used in animal production systems and their effect on the gastrointestinal system of young animals is insufficiently studied. Total lactose inclusion or its partial replacement with dextrose increases intestinal osmotic pressure, which may compromise gastrointestinal barrier function. In this experiment, we investigated the effect of increased osmolality of calf milk replacer (CMR) on gastrointestinal permeability in 30 Holstein Friesian (n = 17) or crossbred (n = 13) bull calves. Read More

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November 2018

Characterization of the partition rate of ibuprofen across the water-octanol interface and the influence of common pharmaceutical excipients.

J Pharm Sci 2018 Nov 21. Epub 2018 Nov 21.

NCE-Formulation Sciences, Drug Product Development Abbvie Inc. 1 North Waukegan Road, North Chicago, IL 60064. Electronic address:

This work reports the measurement of the partition rate of a model drug, ibuprofen, from aqueous solutions into octanol with the absence and presence of common pharmaceutical excipients including glucose, lactose, maltoheptaose, HPMC, PVP, PVP-VA, HPMCAS, sodium dodecyl sulfate, Tween 80, and sodium taurocholate at varying concentrations. This attempts to assess the kinetic aspect of IBU partitioning across the water-octanol interface by applying a mechanistic model and to characterize the interfacial resistance. A significant reduction of the Pi across the water-octanol interface was observed with extremely low concentration of the selected excipients in the aqueous media. Read More

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November 2018
5 Reads
2.590 Impact Factor

Evaluation of rumination time, subsequent yield, and milk trait changes dependent on the period of lactation and reproductive status of dairy cows.

Pol J Vet Sci 2018 Sep;21(3):567-572

Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės 18, Kaunas, Lithuania.

The aim of this research was to determine rumination time (RT) and the subsequent milk yield, along with trait changes during lactation dependent on the reproductive status of dairy cows. 728 cows were selected for evaluation in regards to 1-150 days of milk production (DIM). According to their period of lactation and reproductive status, the cows were selected for the following groups: Inseminated (1-35 days after insemination, n=182), Open (45-90 days after calving, n=126), Fresh (1-44 days after calving, n=45); Not-pregnant (>35-60 days after inse- mination and not-pregnant, n=55); Pregnant (35-60 days after insemination and pregnant (n=320). Read More

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September 2018

Chemical characterization of the milk oligosaccharides of some Artiodactyla species including giraffe (Giraffa camelopardalis), sitatunga (Tragelaphus spekii), deer (Cervus nippon yesoensis) and water buffalo (Bubalus bubalis).

Glycoconj J 2018 12 22;35(6):561-574. Epub 2018 Nov 22.

Department of Life and Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.

Mammalian milk/colostrum usually contains oligosaccharides along with the predominant disaccharide lactose. It has been found that the number and identity of these milk oligosaccharides varies among mammalian species. Oligosaccharides predominate over lactose in the milk/colostrum of Arctoidea species (Carnivora), whereas lactose predominates over milk oligosaccharides in Artiodactyla including cow, sheep, goat, camel, reindeer and pig. Read More

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December 2018

Synthesis of Fucose-Containing Disaccharides by Glycosylhydrolases from Various Origins.

Appl Biochem Biotechnol 2018 Nov 23. Epub 2018 Nov 23.

Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, México D.F., 09340, México.

Glycosylhydrolases of various origins were used to produce fucose-containing disaccharides with prebiotic potential using different donor substrates and L-fucose as the acceptor substrate. Eight different disaccharides were synthesized as follows: three β-D-galactosyl-L-fucosides with glycosidase CloneZyme Gly-001-02 using D-lactose as a donor substrate, two with a structure similar to prebiotics; one β-D-galactosyl-L-fucose with β-D-galactosidase from Aspergillus oryzae using D-lactose as a substrate donor; and four α-D-glucosyl-L-fucosides with α-D-glucosidase from Saccharomyces cerevisiae using D-maltose as a donor substrate. All disaccharides were purified and hydrolyzed. Read More

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November 2018
1 Read

Diet and the Role of Food in Common Gastrointestinal Diseases.

Med Clin North Am 2019 Jan;103(1):101-110

Department of Internal Medicine, University of Texas Southwestern, 5323 Harry Hines Boulevard, Dallas, TX 75390-9151, USA.

Food plays an essential role in normal cellular processes; however, certain foods may also trigger or worsen certain disease states. This article focuses particularly on the role of food in common gastrointestinal and liver diseases, and discusses the current evidence that either supports or debunks common dietary recommendations. Nutrition topics discussed include the use of artificial sweetener for weight loss, avoidance of all dairy products in the setting of lactose intolerance, dietary recommendations for diverticular disease, and dietary management in cirrhotic patients with hepatic encephalopathy. Read More

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January 2019
1 Read

Enhanced production, overexpression and characterization of a hyperthermophilic multimodular GH family 2 β‑glucuronidase (TpGUS) cloned from Thermotoga petrophila RKU-1 in a mesophilic host.

Int J Biol Macromol 2018 Nov 20;123:1132-1142. Epub 2018 Nov 20.

Institute of Industrial Biotechnology, GC University, Lahore 54000, Pakistan.

A multimodular hyperthermophilic β‑glucuronidase (TpGUS) from Thermotoga petrophila RKU-1, belongs to glycoside hydrolase family 2 (GH2), was cloned and overexpressed in Escherichia coli BL21 CodonPlus (DE3)-RIPL. Expression and production of extracellular TpGUS was enhanced through various specific cultivation and induction strategies. Extracellular TpGUS activity was improved by 3. Read More

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November 2018
2 Reads

Changes in the nutrients of camels' milk alter the functional features of the intestine microbiota.

Food Funct 2018 Dec;9(12):6484-6494

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.

Heat treatment alters the nutritive quality of camels' milk and thus the intestine microbiota, but the effect of heat treatment-induced nutrient loss on the functional features of the intestine microbiota is unknown. In this study, the influence of two heat treatments of camels' milk on the intestine microbiota was investigated to establish the correlations between milk nutrients and the functional features of the intestine microbiota. Camels' milk, heat treated at low (65 °C) or high (100 °C) temperatures, was administered to mice (LM = low; HM = high); control mice received sterile distilled water (CW = control). Read More

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December 2018

Spectrum Of Bacterial Culture And Drug Sensitivity Vs Resistance In Uncomplicated Urinary Tract Infection.

J Ayub Med Coll Abbottabad 2018 Jul-Sep;30(3):432-438

Department of Pathology, Ayub Medical College, Abbottabad, Pakistan.

Background: The growing antibiotic resistance against uropathogens has made its treatment a challenge for the physicians. This study was conducted to know the spectrum of bacteria responsible for urinary tract infection and their susceptibility and resistance to available antibiotics.

Methods: This hospital based cross-sectional study was carried out from May to October 2017. Read More

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November 2018
7 Reads

2LARTH, a micro-immunotherapy medicine, exerts anti-inflammatory effects in vitro and reduces TNF-α and IL-1β secretion.

J Inflamm Res 2018 29;11:397-405. Epub 2018 Oct 29.

Clinical Affairs, Labo'Life Belgium, Gembloux, Belgium.

Background: Tumor necrosis factor-α (TNF-α) and IL-1β are 2 pro-inflammatory cytokines known to be involved in rheumatic diseases. The therapeutic strategy used in micro-immunotherapy (MI) to reduce chronic inflammation and attenuate pain consists in mainly targeting these 2 cytokines. 2LARTH is a sublingually administered medicine consisting of lactose-saccharose globules impregnated with ethanolic preparations of immune mediators and nucleic acids at ultra-low doses. Read More

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October 2018
2 Reads

Analysis of lactase in lactose intolerance supplements.

Biochem Mol Biol Educ 2018 Nov;46(6):652-662

Department of Chemistry, College of Science, Technology, Engineering and Mathematics, Youngstown State University, Youngstown, Ohio.

Lactase is the enzyme responsible for the digestion of the disaccharide lactose, and deficiency in this enzyme causes the prevalent medical condition lactose intolerance. Management of lactose intolerance can be achieved through the administration of lactase supplements. Lactase is an appropriate platform for advanced enzymatic study because its medical application is a motivator for student learning. Read More

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November 2018
8 Reads

The Effect of Radiotherapy for Treatment of Head and Neck Cancer on Oral Flora and Saliva.

Oral Health Prev Dent 2018 ;16(5):425-429

Purpose: Radiotherapy causes xerostomia in patients treated for head and neck cancer. This study investigated changes in quality and quantity of saliva after radiotherapy and possible associations between these changes and alterations in oral flora.

Materials And Methods: The study was a prospective cohort study of patients receiving radiotherapy for head and neck cancer. Read More

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January 2018
2 Reads

Increase in anti-apoptotic molecules, nucleolin, and heat shock protein 70, against upregulated LRRK2 kinase activity.

Anim Cells Syst (Seoul) 2018 12;22(5):273-280. Epub 2018 Sep 12.

InAm Neuroscience Research Center, Sanbon Medical Center, College of Medicine, Wonkwang University, Gunpo-si, Republic of Korea.

Leucine-rich repeat kinase 2 (LRRK2) is involved in Parkinson's disease (PD) pathology. A previous study showed that rotenone treatment induced apoptosis, mitochondrial damage, and nucleolar disruption via up-regulated LRRK2 kinase activity, and these effects were rescued by an LRRK2 kinase inhibitor. Heat-shock protein 70 (Hsp70) is an anti-oxidative stress chaperone, and overexpression of Hsp70 enhanced tolerance to rotenone. Read More

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September 2018
1 Read

Influence of moisture variation on the performance of Raman spectroscopy in quantitative pharmaceutical analyses.

J Pharm Biomed Anal 2018 Oct 15;164:528-535. Epub 2018 Oct 15.

Duquesne University Graduate School of Pharmaceutical Sciences, Pittsburgh, PA 15282, United States; Duquesne University Center for Pharmaceutical Technology, Pittsburgh, PA 15282, United States. Electronic address:

To develop a robust quantitative calibration model for spectroscopy, different sources of variability that are not directly related to the components of interest should be included in the calibration samples; this variability should be similar to that which is anticipated during validation and routine operation. Moisture content of pharmaceutical samples can vary as a function of supplier, storage conditions, geographic origin or seasonal variation. Additionally, some pharmaceutical operations (e. Read More

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October 2018

Pharmacological stress impairs working memory performance and attenuates dorsolateral prefrontal cortex glutamate modulation.

Neuroimage 2018 Nov 17;186:437-445. Epub 2018 Nov 17.

Brain Imaging Research Division, Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA.

Working memory processes are associated with the dorsolateral prefrontal cortex (dlPFC). Prior research using proton functional magnetic resonance spectroscopy (H fMRS) observed significant dlPFC glutamate modulation during letter 2-back performance, indicative of working memory-driven increase in excitatory neural activity. Acute stress has been shown to impair working memory performance. Read More

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November 2018
3 Reads
6.360 Impact Factor

Adaptive Evolution of Industrial Under Cell Envelope Stress Provides Phenotypic Diversity.

Front Microbiol 2018 5;9:2654. Epub 2018 Nov 5.

DairySafe Group, Instituto de Productos Lácteos de Asturias (IPLA)-Consejo Superior de Investigaciones Científicas (CSIC), Villaviciosa, Spain.

is widely used as a starter in the manufacture of cheese and fermented milk. Its main role is the production of lactic acid, but also contributes to the sensory attributes of cheese. Unfortunately, the diversity of suitable strains to be commercialized as dairy starters is limited. Read More

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November 2018
2 Reads

Yogurt and other fermented foods as sources of health-promoting bacteria.

Nutr Rev 2018 Dec;76(Supplement_1):4-15

Department of Food Science and Technology, University of Nebraska, Lincoln, Nebraska, USA.

Increased consumption of yogurt, kefir, and other fermented foods has been driven, in part, by the health benefits these products may confer. Epidemiological studies have shown that the consumption of fermented foods is associated with reduced risks of type 2 diabetes, metabolic syndrome, and heart disease, along with improved weight management. The microorganisms present in these foods are suggested to contribute to these health benefits. Read More

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December 2018
15 Reads

Effects of Sodium and Chloride source and concentration on nursery pig growth performance.

J Anim Sci 2018 Nov 19. Epub 2018 Nov 19.

Department of Animal Sciences and Industry, College of Agriculture, Manhattan.

Three studies were conducted to determine the effects of source and concentration of Na and Cl on pig growth performance from 7 to 12 kg. In all 3 experiments, pigs were fed a common diet (0.33% Na and 0. Read More

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November 2018
6 Reads