221 results match your criteria Laboratory Hematology [Journal]


Antineutrophil cytoplasmic antibody-associated neutropenia in a patient with type 1 diabetes mellitus.

Lab Hematol 2014 Jun;20(2):7-8

Department of Internal Medicine, Division of Rheumatology, Istanbul University Cerahpasa Medical Faculty, Istanbul, Turkey.

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http://dx.doi.org/10.1532/LH96.13001DOI Listing
June 2014
13 Reads

Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells.

Lab Hematol 2014 Mar;20(1):2-6

Manitoba Institute of Cell Biology, Cancer Care Manitoba, The Genomic Centre for Cancer Research and Diagnosis, The University of Manitoba, Winnipeg, Manitoba, Canada.

We describe a method to isolate small mononucleated Hodgkin (H) cells from multinucleated Reed Sternberg (RS) cells of Hodgkin lymphoma using the ScreenCell filter device. This filtration-based approach lends itself to future clinical applications in that it enables the separation of H and RS cells from lymph node biopsies, bone marrow aspirates, pleural effusions, and blood, including the isolation of monoclonal Hodgkin precursor cells from the blood. Read More

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http://dx.doi.org/10.1532/LH96.12023DOI Listing
March 2014
19 Reads

Induction of fetal hemoglobin as a novel therapeutic strategy for β-hemoglobinopathy.

Lab Hematol 2014 Mar;20(1)

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands.

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http://dx.doi.org/10.1532/LH96.12015DOI Listing
March 2014
8 Reads

Pathogenetic role of Factor VII deficiency and thrombosis in cross-reactive material positive patients.

Lab Hematol 2013 Dec;19(4):17-21

Department of Medicine, University of Padua Medical School, Padua, Italy.

Congenital Factor VII (FVII) deficiency can be divided into two groups: cases of "true" deficiency, or cross-reactive material (CRM) negative and variants that are cross-reactive material positive.The first form is commonly recognized as Type I condition whereas the second one is known as Type II. FVII deficiency has been occasionally associated with thrombotic events, mainly venous. Read More

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http://dx.doi.org/10.1532/LH96.12011DOI Listing
December 2013
5 Reads

The role of the nervous system in hematopoietic stem cell mobilization.

Lab Hematol 2013 Sep;19(3):8-16

Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Hematopoietic stem cells (HSCs) and blood cell progenitors, such as maturing leucocytes, steadily enter from bone marrow (BM) into the circulation under steady-state conditions, and their mobilization is dramatically amplified during stress conditions and by mediators such as granulocyte colony-stimulating factor (G-CSF). This mobilization is dependent upon bone remodeling, the proteolytic enzymes of bone marrow-derived stromal cells, and adhesion molecules such as integrin, but the main mechanisms controlling this traffic are still unclear. The nervous system, as the most important regulator of the body, can affect the mobilization network by secreting catecholamines, so that denervation of catecholaminergic fibers in the BM of mice could lead to declining mobilization in steady state and stress situations, even in the presence of other intact environmental factors in the BM. Read More

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http://dx.doi.org/10.1532/LH96.12013DOI Listing
September 2013
7 Reads

Evaluation of H3 histone methylation and colony formation in erythroid progenitors treated with thalidomide and sodium butyrate.

Lab Hematol 2013 Mar;19(1):1-5

Department of Hematology and Blood Banking, Tarbiat Modares University, Tehran, Iran.

Objectives: β-thalassemia and sickle cell disease are hemoglobinopathies with reduced/absent β chains in the former and dysfunctional β chains in the latter. In both conditions, up-regulation of hemoglobin F through demethylation can alleviate the symptoms. This can be attained with drugs such as thalidomide and sodium butyrate. Read More

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http://rct.ajums.ac.ir/_rct/documents/H3_20131214_120223.pdf
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http://dx.doi.org/10.1532/LH96.12003DOI Listing
March 2013
8 Reads

Biology and bioinformatics of myeloma cell.

Lab Hematol 2012 Dec;18(4):30-41

Department of Hematology and Blood Banking, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Multiple myeloma (MM) is a plasma cell disorder that occurs in about 10% of all hematologic cancers. The majority of patients (99%) are over 50 years of age when diagnosed. In the bone marrow (BM), stromal and hematopoietic stem cells (HSCs) are responsible for the production of blood cells. Read More

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http://dx.doi.org/10.1532/LH96.11003DOI Listing
December 2012
17 Reads

Infantile pyknocytosis: an under-recognized form of neonatal hemolytic anemia?

Lab Hematol 2012 Dec;18(4):27-9

Pediatric Hematology Unit, Centro Hospitalar do Porto, Porto, Portugal.

Infantile pyknocytosis (IP) is an under-recognized hematological entity of newborns that can cause a severe neonatal hemolytic anemia. A careful, prompt, and accurate peripheral blood smear examination is essential to establish the diagnosis. Here we describe the clinical features and histological parameters of 1 case of IP. Read More

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http://dx.doi.org/10.1532/LH96.12005DOI Listing
December 2012
123 Reads

Platelet hyperreactivity: report of a case with catastrophic arterial thrombosis in a child.

Lab Hematol 2012 Dec;18(4):22-6

Department of Pathology, The Ohio State University, Columbus, OH, USA.

Platelet hyperreactivity is an important but under-recognized cause of idiopathic arterial thrombosis. We describe a case of arterial thrombosis in a previously healthy 12-year-old girl after minor trauma to the knee, resulting in lower extremity amputation. Family history was positive for arterial and venous thrombosis, but an extensive work-up for the usual inherited thrombophilia risk factors was negative. Read More

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http://dx.doi.org/10.1532/LH96.12006DOI Listing
December 2012
8 Reads

Best practices for use of the HEMOX analyzer in the clinical laboratory: quality control determination and choice of anticoagulant.

Lab Hematol 2012 Sep;18(3):17-9

Associated Regional University Pathologists Laboratories, Salt Lake City, Utah, USA.

The HEMOX Analyzer (TCS Scientific) has been used to measure the full oxygen-dissociation curve (ODC) and to calculate P(50) and the Hill coefficient. The effects of different anticoagulants on sample stability and P(50) values have not been evaluated extensively for this instrument. We characterized an artificial hemoglobin (Equil QC463) for quality control (QC) and compared P(50) values for blood samples drawn into 3 different anticoagulants (acid citrate dextrose [ACD], heparin, and EDTA). Read More

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http://dx.doi.org/10.1532/LH96.12001DOI Listing
September 2012
7 Reads

Reactive hemophagocytic syndrome caused by Rickettsial infection: report of a case.

Lab Hematol 2012 Jun;18(2):14-6

Hematology Laboratory Department, Ibn Rochd Medical Center, University of Casablanca, Morocco.

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http://dx.doi.org/10.1532/LH96.11008DOI Listing
June 2012
2 Reads

Clinically silent massive fetomaternal hemorrhage: important lessons from an illustrative case.

Lab Hematol 2012 Jun;18(2):11-3

Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.

Massive fetomaternal hemorrhage (FMH) >150 mL is rare and may occur in the absence of high-risk obstetrical events. The significance of FMH in Rh D-negative women is alloimmunization with an increased risk of hemolytic disease of the newborn in subsequent Rh D-positive pregnancies and adverse outcomes for the fetus/neonate. The Kleihauer-Betke (KB) acid elution test is used to quantify fetal erythrocytes in the circulation of Rh D-negative women postpartum and to calculate the dose of Rh immune globulin (RhIG) needed for prophylaxis against alloimmunization. Read More

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http://dx.doi.org/10.1532/LH96.12002DOI Listing
June 2012
7 Reads

Study of survivin and X-linked inhibitor of apoptosis protein (XIAP) genes in acute myeloid leukemia (AML).

Lab Hematol 2012 Mar;18(1):1-10

Department of Clinical and Chemical Pathology, Cairo University.

Apoptosis deregulation is important for cancer development, chemotherapy response, and prognosis. Survivin and X-linked inhibitor of apoptosis protein (XIAP) are 2 members of the inhibitor of apoptosis proteins family (IAP). We used semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to determine the levels of expression of survivin and XIAP in 30 patients with de novo acute myeloid leukemia (AML) and 20 age- and sex-matched healthy volunteers. Read More

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http://dx.doi.org/10.1532/LH96.11005DOI Listing
March 2012
4 Reads

Burkitt's leukemia with an atypical immunophenotype: report of a case and review of literature.

Lab Hematol 2011 Dec;17(4):27-31

Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, OH, USA.

Burkitt's leukemia (BL) constitutes a small but important fraction of acute leukemias in children. It is an aggressive type of leukemia that is responsive to high-intensity, short-duration chemotherapy with complete remission possible in 75% to 90% of cases. The recognition and proper designation of BL is important because treatment differs from that of precursor B-cell acute lymphoblastic leukemia (pre-B ALL). Read More

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http://dx.doi.org/10.1532/LH96.11004DOI Listing
December 2011
13 Reads

Detection of morphologic changes in peripheral mononuclear cells in hepatitis B virus infection using the beckman coulter LH 750.

Authors:
Y Zhu X Cao D Xu

Lab Hematol 2011 Sep;17(3):22-6

Center of Clinical Laboratory Medicine, Second Affiliated Hospital of Nantong University, Nantong, China.

The Beckman Coulter LH 750 with VCS technology can determine intrinsic biophysical properties of peripheral leukocytes in their "near native state." We investigated the possibility of the LH 750 to detect morphologic changes in lymphocytes/monocytes in hepatitis B virus (HBV) infection. The VCS data from 37 active HBV patients, 140 HBV carriers, and 655 controls were analyzed. Read More

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http://dx.doi.org/10.1532/LH96.09013DOI Listing
September 2011
2 Reads

Similarities and discrepancies in homozygous factor VII defects due to mutations in the region of residues Met298 to Cys310 (exon 8) in the catalytic domain of factor VII.

Lab Hematol 2011 Jun;17(2):17-21

Department of Medical and Surgical Sciences, University of Padua Medical School, Padua, Italy.

Patients with the Arg304Gln mutation in factor VII Padua (FVII Padua) show discrepant activity levels that depend on the thromboplastin used in the assay system. This report investigates the possibility that residues close to Arg304 (exon 8) show the same discrepant behavior. All available homozygous patients with a mutation in a 13-residue region (preceding and following Arg304) have been evaluated. Read More

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http://dx.doi.org/10.1532/LH96.11-001DOI Listing
June 2011
5 Reads

What is the role of JAK2(V617F) mutation in leukemic transformation of myeloproliferative neoplasms?

Lab Hematol 2011 Mar;17(1):12-6

Serviço de Hematologia, Hospital Santo António dos Capuchos-CHLC, Lisboa, Portugal.

Background And Objectives: The role of the Janus kinase 2 V617F (JAK2(V617F)) mutation in the pathogenesis of the various BCR-ABL1-negative myeloproliferative neoplasms (MPNs) remains unclear. Its significance in leukemic transformation is a matter of even greater controversy. The aim of this study was to evaluate both the JAK2(V617F) mutational status of the rare cases in which blast crisis occurred in our institution and the response after intensive treatment. Read More

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http://dx.doi.org/10.1532/LH96.10018DOI Listing
March 2011
3 Reads

Prevalence of heparin in samples submitted for lupus anticoagulant testing.

Lab Hematol 2011 Mar;17(1):6-11

Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA.

Introduction: Lupus anticoagulant (LA) testing can be affected by the presence of anticoagulant medications such as heparin, and current guidelines recommend caution when performing and interpreting LA testing for patients who are receiving anticoagulation treatment. We searched our reference laboratory database to determine the prevalence of heparin in samples submitted for LA testing.

Methods: We reviewed 18,676 LA reflexive testing panels. Read More

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http://dx.doi.org/10.1532/LH96.10024DOI Listing
March 2011
3 Reads

Leukocyte differential for acute abdominal pain in adults.

Lab Hematol 2011 Mar;17(1):1-5

Service de Gériatrie et Médecine Interne, Centre Hospitalier Universitaire de Nancy, Hôpitaux de Brabois, Vandoeuvre, France.

Background: Abdominal pain is a common symptom underlying a variety of disorders with different prognoses. Neutrophilia or lymphopenia have been used as prognostic markers in several stress- or infection-mediated disorders. We studied the clinical relevance of the leukocyte differential in the initial workup of adult patients presenting with abdominal pain. Read More

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http://dx.doi.org/10.1532/LH96.10023DOI Listing
March 2011
9 Reads

Evaluation of peroxidase activity by alpha-naphthol/pyronine staining compared with benzidine staining in 101 acute leukemia cases.

Lab Hematol 2010 Dec;16(4):76-82

University Teaching Hospital, Nancy, France.

Cytochemical detection of myeloperoxidase (MPO) activity, a strong marker for myeloid differentiation, is usually performed by benzidine dihydrochloride staining, with the threshold at 3%. Several reports have demonstrated the potential toxicity of benzidine, and bans have been issued, under French law, prohibiting female technicians from being exposed to the aromatic hydrocarbon group, including benzidine. The aim of this study was to test an alpha-naphthol and pyronine-based substitute using a standardized kit (MYELOPEROXIDASE KIT, RAL [Réactifs RAL, Martillac, France]) to measure MPO activity in blast cells. Read More

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http://dx.doi.org/10.1532/LH96.10007DOI Listing
December 2010
31 Reads

Review: Stem cells and gene therapy.

Lab Hematol 2010 Sep;16(3):53-73

College of Applied Medical Sciences, Al-Kharj University, Al-Kharj, Saudi Arabia.

Both stem cell and gene therapy research are currently the focus of intense research in institutions and companies around the world. Both approaches hold great promise by offering radical new and successful ways of treating debilitating and incurable diseases effectively. Gene therapy is an approach to treat, cure, or ultimately prevent disease by changing the pattern of gene expression. Read More

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http://dx.doi.org/10.1532/LH96.10010DOI Listing
September 2010
4 Reads

A practical approach to the flow cytometric detection and diagnosis of T-cell lymphoproliferative disorders.

Lab Hematol 2010 Sep;16(3):32-52

Department of Pathology and Ireland Cancer Center of Case Western Reserve University/University Hospitals Case Medical Center, Cleveland, Ohio 44106 , USA.

The flow cytometric analysis of T-cell malignancies is difficult due to the heterogeneity of T-cells and the lack of convenient methods to detect T-cell clonality. Neoplastic T-cells are most often detected by their altered level of surface antigen expression, and detection requires an extensive knowledge of the phenotype of normal T-lymphocytes. This review focuses on the methods to distinguish malignant T-cells from their normal counterparts and the phenotypic features of the T-cell lymphoproliferative disorders. Read More

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http://dx.doi.org/10.1532/LH96.10001DOI Listing
September 2010
8 Reads

Do we need time adjusted mean platelet volume measurements?

Lab Hematol 2010 Sep;16(3):28-31

Deptartment of Anaesthesiology and Pain Treatment, Maastricht University Medical Center, Maastricht, the Netherlands.

Mean platelet volume (MPV) is associated with various diseases. Several authors reported anticoagulant and time dependency. Therefore, standardized laboratory methods are essential. Read More

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http://dx.doi.org/10.1532/LH96.10011DOI Listing
September 2010
24 Reads

Blast flagging with the UniCel DxH 800 Coulter Cellular Analysis System.

Lab Hematol 2010 Jun;16(2):23-5

Clinical Hematology, Department of Laboratories, Barnes-Jewish Hospital, St. Louis, Missouri 63110, USA.

We recently conducted a series of evaluations of the new UniCel DxH 800 Coulter Cellular Analysis System at Barnes-Jewish Hospital in St. Louis, Missouri. This report addresses issues relating to the flagging performance of the DxH 800, especially as it relates to the detection of blast cells. Read More

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http://dx.doi.org/10.1532/LH96.09015DOI Listing
June 2010
49 Reads

Identification and selective depletion of alloreactive T-cells for adoptive immunotherapy.

Lab Hematol 2010 Mar;16(1):8-22

University Children's Hospital of Frankfurt/Main, Frankfurt/Main, Germany.

Background: T-cell-depleted allografts may exhibit delayed T-cell recovery, severe infections, and relapse after haploidentical hematopoietic stem cell transplantation (HSCT). Required donor lymphocyte infusions containing nonalloreactive cells may transfer immune function without causing graft-versus-host disease.

Methods: We developed an ex vivo approach for the immunomagnetic depletion of alloreactive CD25+, CD69+, and HLA-DR+ T-cells. Read More

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http://dx.doi.org/10.1532/LH96.09010DOI Listing
March 2010
2 Reads

Reticulocyte count and reticulocyte maturation profile in human umbilical cord blood from healthy newborns.

Lab Hematol 2010 Mar;16(1):3-7

Department of Laboratory Medicine, AO Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.

Most fetal hematologic parameters show a significant relationship with gestational age: a linear increase is evident throughout gestation for several hematologic parameters. A few reports have described reference values for umbilical cord blood reticulocyte counts performed with automated hematology analyzers. Our aim was to use automated hematology analyzers (ADVIA 120; Siemens Healthcare Diagnostics) to establish reference intervals for reticulocyte parameters in cord blood from healthy newborns of 34 to 42 weeks of gestation. Read More

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http://dx.doi.org/10.1532/LH96.09012DOI Listing
March 2010
122 Reads

Effects of EDTA on routine and specialized coagulation testing and an easy method to distinguish EDTA-treated from citrated plasma samples.

Lab Hematol 2009 ;15(4):45-8

ARUP Institute for Clinical and Experimental Pathology, Salt Lake City ARUP Hemostasis/Thrombosis Laboratory, Salt Lake City, UT 84108, USA.

Coagulation testing is performed with citrate-treated plasma. Samples submitted in other anticoagulants, such as EDTA, should not be tested. We aimed to evaluate the effects of EDTA on routine and specialized coagulation tests and to establish sodium tetraphenylborate testing as a quick and reliable method to identify EDTA-treated plasma samples. Read More

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http://dx.doi.org/10.1532/LH96.09013DOI Listing
February 2010
91 Reads

Indications and diagnostic utility of bone marrow examination in different bone marrow disorders in Iran.

Lab Hematol 2009 ;15(4):38-44

Department of Pathology, Rasoul Akram Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

The shortage of published literature regarding the indications for bone marrow examination and the prevalence of hematologic disorders in the Middle East, especially Iran, prompted us to review 1154 bone marrow specimens sent to the Pathology Department of Rasoul Akram Hospital in Tehran from January 2002 to December 2006. We aimed to determine the diagnostic utility of the bone marrow examination for different bone marrow disorders by determining the clinical suspicion and indications for bone marrow examination for each patient and comparing these findings with the final pathologic diagnoses. The patients comprised 657 males and 497 females, with ages ranging from 1 to 95 years. Read More

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http://dx.doi.org/10.1532/LH96.09009DOI Listing
February 2010
4 Reads

False-positive crystals in urine from contamination by Multistix 10 SG reagent strips for urinalysis.

Lab Hematol 2009 ;15(4):35-7

Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.

Dipstick testing of urine is commonly performed as part of point-of-care testing. Unless separate aliquots are used for dipstick testing, substances may be eluted from the test strip and contaminate patient samples. Such contamination has the potential to lead to interference in subsequent assays if the contaminated specimen is sent to the central laboratory for additional testing. Read More

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http://dx.doi.org/10.1532/LH96.09008DOI Listing
February 2010
6 Reads

The coinheritance of beta- and alpha- thalassemia: a review of one patient and her family.

Lab Hematol 2009 ;15(3):30-3

Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, Ohio 43205, USA.

The diagnosis and management of alpha-thalassemia may be complicated by the variability of the phenotype, which is due to the interaction of coinherited alpha-thalassemia and the variable severity of beta-thalassemia mutations. A well-documented case of complex beta- and alpha-thalassemia coinheritance is described. Laboratory and clinical data for the patient and her family are reviewed. Read More

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http://dx.doi.org/10.1532/LH96.09002DOI Listing
December 2009
2 Reads

Ethnic and genetic causes of neutropenia: clinical and therapeutic implications.

Authors:
Guido D'Angelo

Lab Hematol 2009 ;15(3):25-9

Ematologia/Coagulazione, Laboratorio di Chimica-Clinica, Ematologia e Microbiologia, Azienda Ospedaliera S. Antonio Abate, Varese, Italy.

The white blood cell count represents clinical data linked with different pathologic conditions, as well as with lifestyle. Another very important condition that affects the number of leukocytes is race and ethnic group-the geographic zone of origin. Genetic studies have identified the gene that controls the expression of the Duffy antigen receptor for chemokine (DARC), which is associated with the ethnic group to which individuals belong. Read More

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http://dx.doi.org/10.1532/LH96.09005DOI Listing
December 2009
4 Reads

Compound heterozygosity for hemoglobin S [beta6(A3)Glu6Val] and hemoglobin Korle-Bu [beta73(E17)Asp73Asn].

Lab Hematol 2009 ;15(3):20-4

Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30303, USA.

We report a case of compound heterozygous hemoglobins S [beta6(A3)Glu6Val] and Korle-Bu [beta73(E17)Asp73Asn] in a 2-year-old girl. This hemoglobin genotype is associated with a benign clinical course, much like the sickle cell trait; however, its laboratory characteristics are very similar to compound heterozygous hemoglobin S and hemoglobin D-Los Angeles [beta121(GH4)Glu121Gln], which produces severe sickling hemolytic anemia. We describe laboratory data used to resolve this important differential diagnosis and review the interactions between hemoglobin S and the variant hemoglobins that may account for the different clinical phenotypes in compound heterozygotes. Read More

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http://dx.doi.org/10.1532/LH96.09004DOI Listing
December 2009
9 Reads

Usefulness of immature platelet fraction for the clinical evaluation of myelodysplastic syndromes.

Lab Hematol 2009 ;15(2):13-6

Faculty of Pharmacological Science, Himeji Dokkyo University, Himeji, Japan.

Ratios of young platelets or reticulated platelets can be routinely obtained as an immature platelet fraction (IPF) with the XE-2100 automated hematology analyzer (Sysmex, Kobe, Japan). We combined IPF analysis of 31 patients with myelodysplastic syndrome (MDS) with a complete blood count, a bone marrow examination, and a chromosome analysis. The patients with >40 x 10(9)/L platelets were classified as group A, and those with > or =40 x 10(9)/L were placed in group B. Read More

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http://dx.doi.org/10.1532/LH96.09003DOI Listing
September 2009
7 Reads

An effective method for hemoglobin E detection: DEAE sepharose microcolumn.

Lab Hematol 2009 ;15(2):10-2

Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, Thailand.

Hemoglobin E (Hb E) is a variant hemoglobin that can lead to considerable morbidity when it occurs in a compound heterozygous state with beta-thalassemia. Therefore, its detection is important because it permits antenatal counseling. Hb E is prevalent in economically weaker regions of the world. Read More

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http://dx.doi.org/10.1532/LH96.08011DOI Listing
September 2009
5 Reads

The association of D-dimer levels with clinical outcomes in patients presenting with acute pulmonary embolism.

Lab Hematol 2009 ;15(1):4-9

Department of Medicine, St. Joseph's Regional Medical Center, Paterson, NJ, USA.

The D-dimer fragment of fibrin degradation has been a useful adjunct in the diagnosis of venous thromboembolism (VTE). In conjunction with predictive algorithms, the high negative predictive value (NPV) of D-dimer measurements has provided this analyte with a prominent position in the diagnosis of pulmonary embolism (PE). The purpose of this study was to determine if D-dimer levels correlate with ventilation/perfusion (V/Q) derangements as assessed by the alveolar-arterial oxygen tension gradient (DeltaA-a) and to ascertain if quantitative measurements of D-dimer on admission have prognostic value in terms of during-admission mortality and recurrence over a 60-week period. Read More

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http://dx.doi.org/10.1532/LH96.08014DOI Listing
April 2009
3 Reads

Aberrant phenotypic expression of the T-cell-associated antigen CD8 on B-cell chronic lymphocytic leukemia cells.

Lab Hematol 2009 ;15(1):1-3

Section of Hematology, Department of Biomedical Sciences, University of Catania, Ferrarotto Hospital, via S. Citelli 6, Catania, Italy.

B-cell chronic lymphocytic leukemia (B-CLL) is a heterogeneous hematologic malignancy caused by a clonally expanded B-cell population that recirculates between the blood and tissues. Described is the case of an 81-year-old female B-CLL patient with aberrant expression of a T-cell-associated antigen, CD8, on CD19+ B-CLL cells. Despite chlorambucil therapy and a subsequent salvage regimen, the patient rapidly entered progressive disease and died 9 months later. Read More

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http://dx.doi.org/10.1532/LH96.08015DOI Listing
April 2009
12 Reads

Ex vivo coagulation test on tissue factor-expressing cells with a calibrated automated thrombogram.

Authors:
O Takamiya M Sakata

Lab Hematol 2008 ;14(4):39-44

Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Asahi 390-8621, Matsumoto, Japan.

A calibrated automated thrombogram is not affected by the turbidity of platelet and cell preparations because the measurement is based on fluorescence. To examine conditions that mimic the physiological state, we investigated thrombograms that show thrombin generation on tissue factor (TF)-bearing cells. An increase in the number of J82 cells did not affect the endogenous thrombin potential (ETP) of normal plasma, although the lag time (LT), the peak height, and the time to peak (ttPeak) did depend on cell concentration. Read More

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http://dx.doi.org/10.1532/LH96.08010DOI Listing
February 2009
10 Reads

EDTA-dependent lymphoagglutination.

Lab Hematol 2008 ;14(4):35-7

Laboratory of Haematology, CHU Nancy-Brabois, Vandoeuvre, France.

Lymphoagglutination is an extremely rare EDTA-dependent phenomenon and is far less frequent than neutrophil agglutination. We describe 6 new cases and briefly review the literature on lymphoagglutination. Read More

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http://dx.doi.org/10.1532/LH96.08006DOI Listing
February 2009
2 Reads

Compound heterozygosity for hemoglobin C and hemoglobin Korle-Bu.

Lab Hematol 2008 ;14(3):30-4

Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30303, USA.

We describe a case of compound heterozygosity for hemoglobin C (beta6 Glu-->Lys) and hemoglobin Korle-Bu (beta73 Asp-->Asn). To our knowledge, this is the third case report of this unusual hemoglobin genotype, and the first to integrate data from cation exchange high-performance liquid chromatography, hemoglobin electrophoresis, and beta-globin gene sequencing. The principal hematological finding in our case was microcytosis without significant anemia. Read More

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http://dx.doi.org/10.1532/LH96.08001DOI Listing
February 2009
3 Reads

Stability tests for hematological parameters in antidoping analyses.

Lab Hematol 2008 ;14(3):24-9

Institute of Biochemistry, German Sports University Cologne, Cologne, Germany.

The purpose of this study was to evaluate the influences of delayed sample analysis on the stability of erythrocyte parameters and reticulocyte parameters for antidoping tests performed on the ADVIA120 system. We analyzed erythrocyte count, hemoglobin, hematocrit, mean cell volume, percentage of hypochromic erythrocytes, percentage of macrocytes, absolute reticulocyte count, percentage of reticulocytes, mean cell volume of reticulocytes, cell hemoglobin of reticulocytes, percentage of high-fluorescent reticulocytes, and OFF-Score during a 48-hour storage period at 4 degrees C or 21 degrees C. Data analysis was performed by fitting linear or nonlinear mixed-effects models. Read More

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http://dx.doi.org/10.1532/LH96.08005DOI Listing
February 2009
3 Reads

Sebastian syndrome with abnormal platelet response to ristocetin.

Lab Hematol 2008 ;14(3):19-23

Department of Pathology, University of Florida, Gainesville, FL 32610-0275, USA.

Background: Sebastian syndrome is characterized by enlarged platelets and Döhle-like body leukocyte inclusions. This syndrome is an MYH-9-related disease, a group that also includes May-Hegglin anomaly and Fechtner syndrome. The differential diagnosis of the MYH-9 diseases requires ultrastructural studies. Read More

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http://dx.doi.org/10.1532/LH96.08007DOI Listing
February 2009
9 Reads

Effect on sport hemolysis of cold water leg immersion in athletes after training sessions.

Lab Hematol 2008 ;14(2):15-8

Dipartimento Tecnologie per Salute, School of Medicine, University of Milan, Milan, Italy.

The principal source of increased turnover of erythrocytes in athletes is sport hemolysis, the intravascular hemolysis that characteristically occurs with athletic performance in sport. The use of the parameter mean sphered cell volume (MSCV), automatically measured by means of the Coulter LH750, could be useful for diagnosing the presence of sport hemolysis. We studied the behavior of MSCV and mean corpuscular volume (MCV) in 30 top-level rugby players who underwent a heavy training session followed by 3 different recovery methods, administered to 3 subgroups of 10 athletes. Read More

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http://dx.doi.org/10.1532/LH96.08004DOI Listing
February 2009
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A case of CD45-negative diffuse large B-cell lymphoma in thyroid gland.

Lab Hematol 2008 ;14(2):12-4

Department of Infection Control and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan.

CD45, also referred to as the leukocyte common antigen (LCA), is selectively expressed on nucleated hematopoietic cells in healthy individuals. It is also widely expressed in various types of hematologic malignancies--although 10% of acute lymphoblastic leukemia and more than 50% of multiple myeloma have been reported to lack its expression. In this paper, we report a rare case of CD45-negative non-Hodgkin's lymphoma (NHL) that affected the thyroid gland. Read More

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http://dx.doi.org/10.1532/LH96.08002DOI Listing
February 2009
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Acute lymphoblastic leukemia with hypereosinophilia and 9p21 deletion: case report and review of the literature.

Lab Hematol 2008 ;14(1):7-9

Laboratorio di Chimica-Clinica, Ematologia e Microbiologia, Ematologia/Coagulazione, Azienda Ospedaliera S. Antonio Abate-Gallarate, Varese, Italy.

Acute lymphoblastic leukemia (ALL) associated with eosinophilia is very rare, with approximately 44 reported cases. We are reporting this case not only because of the rarity of ALL with peripheral blood eosinophilia, but also because we observed a homozygous deletion of the 9p21 locus corresponding to the p16 gene, a cytogenetic abnormality that was not reported in other documented cases. ALL with eosinophilia must be considered a distinct clini-copathologic entity. Read More

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http://cardenjennings.metapress.com/openurl.asp?genre=articl
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http://dx.doi.org/10.1532/LH96.07018DOI Listing
August 2009
4 Reads