6,176 results match your criteria Lab on a Chip [Journal]


Elastic reversible valves on centrifugal microfluidic platforms.

Lab Chip 2019 Feb 20. Epub 2019 Feb 20.

School of Engineering and Sciences, Nanosensor and Devices, Tecnologico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, 64849, Monterrey, NL, Mexico.

Reversible valves on centrifugal microfluidic platforms facilitate the automation of bioanalytical assays, especially of those requiring a series of steps (such as incubation) in a single reaction chamber. In this study, we present fixed elastic reversible (FER) valves and tunable elastic reversible (TER) valves that are easy to fabricate, implement and control. In the FER valve the compression of an elastic barrier/patch against a microchamber's outlet prevents the release of liquid. Read More

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http://xlink.rsc.org/?DOI=C8LC00849C
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http://dx.doi.org/10.1039/c8lc00849cDOI Listing
February 2019
1 Read

Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides.

Lab Chip 2019 Feb 20. Epub 2019 Feb 20.

School of Physics and Astronomy, University of Leeds, Leeds, UK.

We describe a novel biosensor based on phospholipid-coated nematic liquid crystal (LC) droplets and demonstrate the detection of Smp43, a model antimicrobial peptide (AMP) from the venom of North African scorpion Scorpio maurus palmatus. Mono-disperse lipid-coated LC droplets of diameter 16.7 ± 0. Read More

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http://dx.doi.org/10.1039/c8lc01291aDOI Listing
February 2019
1 Read

Rotating magnetic particles for lab-on-chip applications - a comprehensive review.

Lab Chip 2019 Feb 20. Epub 2019 Feb 20.

Department of Applied Physics, Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands.

Magnetic particles are widely used in lab-on-chip and biosensing applications, because they have a high surface-to-volume ratio, they can be actuated with magnetic fields and many biofunctionalization options are available. The most well-known actuation method is to apply a magnetic field gradient which generates a translational force on the particles and allows separation of the particles from a suspension. A more recently developed magnetic actuation method is to exert torque on magnetic particles by a rotating magnetic field. Read More

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http://xlink.rsc.org/?DOI=C8LC01323C
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http://dx.doi.org/10.1039/c8lc01323cDOI Listing
February 2019
1 Read

Formation of precisely composed cancer cell clusters using a cell assembly generator (CAGE) for studying paracrine signaling at single-cell resolution.

Lab Chip 2019 Feb 20. Epub 2019 Feb 20.

Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.

The function and behaviour of any given cell in a healthy tissue, or in a tumor, is affected by interactions with its neighboring cells. It is therefore important to create methods that allow for reconstruction of tissue niches in vitro for studies of cell-cell signaling and associated cell behaviour. To this end we created the cell assembly generator (CAGE), a microfluidic device which enables the organization of different cell types into precise cell clusters in a flow chamber compatible with high-resolution microscopy. Read More

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http://xlink.rsc.org/?DOI=C8LC01153B
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http://dx.doi.org/10.1039/c8lc01153bDOI Listing
February 2019
1 Read

High-throughput mechanotransduction in Drosophila embryos with mesofluidics.

Lab Chip 2019 Feb 19. Epub 2019 Feb 19.

Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Developing embryos create complexity by expressing genes to coordinate movement which generates mechanical force. An emerging theory is that mechanical force can also serve as an input signal to regulate developmental gene expression. Experimental methods to apply mechanical stimulation to whole embryos have been limited, mainly to aspiration, indentation, or moving a coverslip; these approaches stimulate only a few embryos at a time and require manual alignment. Read More

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http://dx.doi.org/10.1039/c8lc01055bDOI Listing
February 2019

Microfluidic assay for the on-chip electrochemical measurement of cell monolayer permeability.

Lab Chip 2019 Feb 19. Epub 2019 Feb 19.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada and Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, MaRS West Tower, 661 University Avenue, 14th Floor, Toronto, ON M5G 1M1, Canada. and Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada.

Cell monolayers, including endothelial cells lining the vasculature and blood-brain barrier, and epithelial cells lining the lung airways and gut, form a semipermeable barrier across which transport of biomolecules is tightly regulated. The assessment of barrier function is therefore critical in in vitro models of barrier-forming tissues, including microfluidic organ-on-a-chip models. Cell monolayer barrier function is commonly assessed using a fluorescent tracer-based permeability assay in both conventional Transwell and organ-on-a-chip models, but this method requires laborious manual sampling, bulky instrumentation and offline sample processing. Read More

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http://dx.doi.org/10.1039/c8lc01321gDOI Listing
February 2019

A novel technique for in situ uniaxial tests of self-assembled soft biomaterials.

Lab Chip 2019 Feb 18. Epub 2019 Feb 18.

University of Illinois at Urbana-Champaign, 1206 W. Green St, Urbana, IL 61801, USA.

We introduce a novel method to form 3D biomimetic tissues from a droplet of a cell-extracellular matrix (ECM) mixture on a sensor stage and to quantify tissue force and stiffness as a function of time under optical microscopes. This method exploits advances in micro-nano fabrication and capillarity for self-assembly and self-alignment of tissues on the stage. It allows simultaneous investigation of the microstructure of the tissue in situ while its mechanical response is quantified, thus linking tissue biophysics with physiology and revealing structural-functional properties of 3D tissues. Read More

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http://xlink.rsc.org/?DOI=C8LC01273C
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http://dx.doi.org/10.1039/c8lc01273cDOI Listing
February 2019
1 Read

Surface acoustic waves enable rotational manipulation of Caenorhabditis elegans.

Lab Chip 2019 Feb 15. Epub 2019 Feb 15.

Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.

Controllable, precise, and stable rotational manipulation of model organisms is valuable in many biomedical, bioengineering, and biophysics applications. We present an acoustofluidic chip capable of rotating Caenorhabditis elegans (C. elegans) in both static and continuous flow in a controllable manner. Read More

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http://dx.doi.org/10.1039/c8lc01012aDOI Listing
February 2019

Gradient in the electric field for particle position detection in microfluidic channels.

Lab Chip 2019 Feb 15. Epub 2019 Feb 15.

BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, University of Twente, Drienerlolaan 5, Enschede, The Netherlands.

In this work, a new method to track particles in microfluidic channels is presented. Particle position tracking in microfluidic systems is crucial to characterize sorting systems or to improve the analysis of cells in impedance flow cytometry studies. By developing an electric field gradient in a two parallel electrode array the position of the particles can be tracked in one axis by impedance analysis. Read More

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http://xlink.rsc.org/?DOI=C8LC01333K
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http://dx.doi.org/10.1039/c8lc01333kDOI Listing
February 2019
1 Read

On-chip phenotypic investigation of combinatory antibiotic effects by generating orthogonal concentration gradients.

Lab Chip 2019 Feb 15. Epub 2019 Feb 15.

Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea. and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.

Combinatory therapy using two or more kinds of antibiotics is attracting considerable attention for inhibiting multi-drug resistant pathogenic bacteria. Although the therapy mostly leads to more powerful antimicrobial effects than using a single antibiotic (synergy), interference may arise from certain antibiotic combinations, resulting in the antimicrobial effect being suppressed (antagonism). Here, we present a microfluidic-based phenotypic screening chip to investigate combinatory antibiotic effects by automatically generating two orthogonal concentration gradients on a bacteria-trapping agarose gel. Read More

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http://dx.doi.org/10.1039/c8lc01406jDOI Listing
February 2019

Tumor cell capture from blood by flowing across antibody-coated surfaces.

Lab Chip 2019 Feb 14. Epub 2019 Feb 14.

Medical Cell Biophysics Group, Technical Medical Centre, Faculty of Science and Technology, University of Twente, The Netherlands.

The load of circulating tumor cells (CTC) is related to poor outcomes in cancer patients. A sufficient number of these cells would enable a full characterization of the cancer. An approach to probe larger blood volumes, allowing for the detection of more of these very rare CTC, is the use of leukapheresis. Read More

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http://dx.doi.org/10.1039/c8lc01158cDOI Listing
February 2019

Performance tuning of microfluidic flow-focusing droplet generators.

Lab Chip 2019 Feb 14. Epub 2019 Feb 14.

Biological Design Center, 610 Commonwealth Avenue, Boston, MA 02215, USA. and Boston University Department of Electrical and Computer Engineering, Boston, USA.

The required step in all droplet-based devices is droplet formation. A droplet generator must deliver an application-specific performance that includes a prescribed droplet size and generation frequency while producing monodisperse droplets. The desired performance is usually reached through several cost- and time-inefficient design iterations. Read More

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http://dx.doi.org/10.1039/c8lc01253aDOI Listing
February 2019

Two-way communication between ex vivo tissues on a microfluidic chip: application to tumor-lymph node interaction.

Lab Chip 2019 Feb 11. Epub 2019 Feb 11.

Department of Chemistry, University of Virginia, Charlottesville, VA, USA. and Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA and Carter Immunology Center, University of Virginia, Charlottesville, VA, USA and University of Virginia Cancer Center, Charlottesville, VA, USA.

Experimentally accessible tools to replicate the complex biological events of in vivo organs offer the potential to reveal mechanisms of disease and potential routes to therapy. In particular, models of inter-organ communication are emerging as the next essential step towards creating a body-on-a-chip, and may be particularly useful for poorly understood processes such as tumor immunity. In this paper, we report the first multi-compartment microfluidic chip that continuously recirculates a small volume of media through two ex vivo tissue samples to support inter-organ cross-talk via secreted factors. Read More

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http://dx.doi.org/10.1039/c8lc00957kDOI Listing
February 2019

An ultrasensitive test for profiling circulating tumor DNA using integrated comprehensive droplet digital detection.

Lab Chip 2019 Feb 8. Epub 2019 Feb 8.

Sue and Bill Gross Stem Cell Research Center, Sue & Bill Gross Hall CIRM Institute, University of California, Irvine, 845 Health Sciences Road, Suite 3027, Irvine, CA 92697, USA. and Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA 92697, USA and Chao Family Comprehensive Cancer Center, University of California, Irvine, Irvine, CA 92697, USA and Edwards Life Sciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, CA 92697, USA and Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA and Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA.

Current cancer detection systems lack the required sensitivity to reliably detect minimal residual disease (MRD) and recurrence at the earliest stages when treatment would be most effective. To address this issue, we present a novel liquid biopsy approach that utilizes an integrated comprehensive droplet digital detection (IC3D) digital PCR system which combines microfluidic droplet partitioning, fluorescent multiplex PCR chemistry, and our rapid 3D, large-volume droplet counting technology. The IC3D ddPCR assay can detect cancer-specific, ultra-rare genomic targets due to large sample input and high degree of partitioning. Read More

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http://dx.doi.org/10.1039/c8lc01399cDOI Listing
February 2019
2 Reads

Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP.

Lab Chip 2019 Feb 8. Epub 2019 Feb 8.

Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.

Digital nucleic acid amplification and detection methods provide excellent sensitivity and specificity and allow absolute quantification of target nucleic acids. Isothermal methods such as digital loop-mediated isothermal amplification (digital LAMP) have potential for use in rapid disease diagnosis in low-resource settings due to their speed and lack of thermal cycling. We previously developed a self-digitization (SD) chip, a simple microfluidics device that automatically digitizes a sample into an array of nanoliter wells, for use in digital LAMP. Read More

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http://dx.doi.org/10.1039/c8lc01223gDOI Listing
February 2019

Tailored 3D printed micro-crystallization chip for versatile and high-efficiency droplet evaporative crystallization.

Lab Chip 2019 Feb 7. Epub 2019 Feb 7.

State Key Laboratory of Fine Chemicals, Engineering Laboratory for Petrochemical Energy-efficient Separation Technology of Liaoning Province, School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning 116024, China.

Droplet evaporative crystallization on a micro-structured platform with limited interfacial area has potential applications in crystallization theory, bioengineering, and particle drug preparation. Here, an efficient and versatile approach is discussed for multiple drop-evaporative crystallization processes on a micro-crystallization chip fabricated via three-dimensional printing. A chip with limited interfacial area could be fabricated on a highly controlled crystallizer interface. Read More

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http://dx.doi.org/10.1039/c8lc01319eDOI Listing
February 2019
1 Read

Paper-based multiplexed vertical flow assay for point-of-care testing.

Lab Chip 2019 Feb 7. Epub 2019 Feb 7.

Electrical & Computer Engineering Department, University of California, Los Angeles, California 90095, USA. and California NanoSystems Institute (CNSI), University of California, Los Angeles, California 90095, USA and Bioengineering Department, University of California, Los Angeles, California 90095, USA.

We developed a multiplexed point-of-care immunodiagnostic assay for antibody detection in human sera made through the vertical stacking of functional paper layers. In this multiplexed vertical flow immunodiagnostic assay (xVFA), a colorimetric signal is generated by gold nanoparticles captured on a spatially-multiplexed sensing membrane containing specific antigens. The assay is completed in 20 minutes, following which the sensing membrane is imaged by a cost-effective mobile-phone reader. Read More

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http://xlink.rsc.org/?DOI=C9LC00011A
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http://dx.doi.org/10.1039/c9lc00011aDOI Listing
February 2019
4 Reads

Point of care technologies for sepsis diagnosis and treatment.

Lab Chip 2019 Feb 6. Epub 2019 Feb 6.

Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, USA. and Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.

Sepsis is a rapidly progressing, life threatening immune response triggered by infection that affects millions worldwide each year. Current clinical diagnosis relies on broad physiological parameters and time consuming lab-based cell culture. If proper treatment is not provided, cases of sepsis can drastically increase in severity over the course of a few hours. Read More

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http://xlink.rsc.org/?DOI=C8LC01102H
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http://dx.doi.org/10.1039/c8lc01102hDOI Listing
February 2019
4 Reads

An integrated device for the rapid and sensitive detection of the influenza hemagglutinin.

Lab Chip 2019 Feb 6. Epub 2019 Feb 6.

Department of Bioengineering, University of Washington, Seattle, WA, USA.

Influenza is a viral respiratory tract infection responsible for up to 5 million cases of severe infection and nearly 600 000 deaths worldwide each year. While treatments for influenza exist, diagnostics for the virus at the point of care are limited in their sensitivity and ability to differentiate between subtypes. We have developed an integrated two-dimensional paper network (2DPN) for the detection of the influenza virus by the surface glycoprotein, hemagglutinin. Read More

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http://xlink.rsc.org/?DOI=C8LC00691A
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http://dx.doi.org/10.1039/c8lc00691aDOI Listing
February 2019
3 Reads

Reliable interfaces for EGaIn multi-layer stretchable circuits and microelectronics.

Lab Chip 2019 Feb 6. Epub 2019 Feb 6.

Institute of Systems and Robotics, University of Coimbra, Rua Silvio Lima-Polo II, 3030-290 Coimbra, Portugal.

We tackle two well-known problems in the fabrication of stretchable electronics: interfacing soft circuit wiring with silicon chips and fabrication of multi-layer circuits. We demonstrate techniques that allow integration of embedded flexible printed circuit boards (FPCBs) populated with microelectronics into soft circuits composed of liquid metal (LM) interconnects. These methods utilize vertical interconnect accesses (VIAs) that are produced by filling LM alloy into cavities formed by laser ablation. Read More

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http://xlink.rsc.org/?DOI=C8LC01093E
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http://dx.doi.org/10.1039/c8lc01093eDOI Listing
February 2019
2 Reads

3D arrays of microcages by two-photon lithography for spatial organization of living cells.

Lab Chip 2019 Feb 6. Epub 2019 Feb 6.

Department of Microsystems Engineering (IMTEK), University of Freiburg, Germany.

This paper addresses a nanoengineering approach to create a fully three-dimensional (3D) network of living cells, providing an advanced solution to in vitro studies on either neuronal networks or artificial organs. The concept of our work relies on stackable scaffolds composed of microcontainers designed and dimensioned to favor the geometrically constrained growth of cells. The container geometry allows cells to communicate in the culture medium and freely grow their projections to form a 3D arrangement of living cells. Read More

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http://xlink.rsc.org/?DOI=C8LC01240G
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http://dx.doi.org/10.1039/c8lc01240gDOI Listing
February 2019
3 Reads

Chemical-PDMS binding kinetics and implications for bioavailability in microfluidic devices.

Lab Chip 2019 Feb 5. Epub 2019 Feb 5.

Department of Physics and Astronomy, Vanderbilt University, Nashville, TN, USA.

Microfluidic organ-on-chip devices constructed from polydimethylsiloxane (PDMS) have proven useful in studying both beneficial and adverse effects of drugs, supplements, and potential toxicants. Despite multiple advantages, one clear drawback of PDMS-based devices is binding of hydrophobic chemicals to their exposed surfaces. Chemical binding to PDMS can change the timing and extent of chemical delivery to cells in such devices, potentially altering dose-response curves. Read More

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http://xlink.rsc.org/?DOI=C8LC00796A
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http://dx.doi.org/10.1039/c8lc00796aDOI Listing
February 2019
2 Reads

Engineering human islet organoids from iPSCs using an organ-on-chip platform.

Lab Chip 2019 Feb 5. Epub 2019 Feb 5.

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China. and Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China and CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China and University of Chinese Academy of Sciences, Beijing, China.

Human pluripotent stem cell (hPSC)-derived islet cells provide promising resources for diabetes studies, cell replacement treatment and drug screening. Recently, hPSC-derived organoids have represented a new class of in vitro organ models for disease modeling and regenerative medicine. However, rebuilding biomimetic human islet organoids from hPSCs remains challenging. Read More

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http://xlink.rsc.org/?DOI=C8LC01298A
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http://dx.doi.org/10.1039/c8lc01298aDOI Listing
February 2019
4 Reads

Rapid imaging, detection, and quantification of Nosema ceranae spores in honey bees using mobile phone-based fluorescence microscopy.

Lab Chip 2019 Feb 5. Epub 2019 Feb 5.

Electrical and Computer Engineering Department, University of California at Los Angeles, Los Angeles, CA 90095, USA. and Bioengineering Department, University of California at Los Angeles, Los Angeles, CA 90095, USA and California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, CA 90095, USA.

Recent declines in honey bee colonies in the United States have put increased strain on agricultural pollination. Nosema ceranae and Nosema apis, are microsporidian parasites that are highly pathogenic to honey bees and have been implicated as a factor in honey bee losses. While traditional methods for quantifying Nosema infection have high sensitivity and specificity, there is no field-portable device for field measurements by beekeepers. Read More

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http://xlink.rsc.org/?DOI=C8LC01342J
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http://dx.doi.org/10.1039/c8lc01342jDOI Listing
February 2019
1 Read

Modular soft robotic microdevices for dexterous biomanipulation.

Lab Chip 2019 Feb 4. Epub 2019 Feb 4.

Institute of Mechanical Engineering and Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

We present a methodology for building biologically inspired, soft microelectromechanical systems (MEMS) devices. Our strategy combines several advanced techniques including programmable colloidal self-assembly, light-harvesting with plasmonic nanotransducers, and in situ polymerization of compliant hydrogel mechanisms. We synthesize optomechanical microactuators using a template-assisted microfluidic approach in which gold nanorods coated with thermoresponsive poly(N-isopropylmethacrylamide) (pNIPMAM) polymer function as nanoscale building blocks. Read More

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http://xlink.rsc.org/?DOI=C8LC01200H
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http://dx.doi.org/10.1039/c8lc01200hDOI Listing
February 2019
1 Read

Transformable bubble-filled alginate microfibers via vertical microfluidics.

Lab Chip 2019 Feb 1. Epub 2019 Feb 1.

Department of Physics, King's College London, Strand, London, WC2R 2LS, UK.

A novel buoyancy-assisted vertical microfluidic setup has been developed to fabricate a new class of transformable bubble-filled hydrogel microfibers. A co-axial flow of an aqueous sodium-alginate solution enveloping an air phase was injected into a quiescent aqueous CaCl2 solution, through a vertically-oriented co-axial glass-capillary setup. This induced instantaneous gelation and produced bubble-filled calcium-alginate fibers. Read More

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http://xlink.rsc.org/?DOI=C8LC01081A
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http://dx.doi.org/10.1039/c8lc01081aDOI Listing
February 2019
2 Reads

Simultaneous electrokinetic stacking and separation of anionic and cationic species on a paper fluidic channel.

Lab Chip 2019 Feb 1. Epub 2019 Feb 1.

Research Center for Analytical Sciences, College of Sciences, Northeastern University, Shenyang 110819, P. R. China.

On-line enrichment is effective for improving the sensitivity of paper-based analytical devices (PADs). Electrokinetic stacking of ionic species - anionic or cationic species, respectively, on a paper-based fluidic channel has been well demonstrated in the literature. In this work, we further demonstrated that both anionic and cationic species can be electrokinetically stacked and separated simultaneously on the same paper fluidic channel. Read More

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http://xlink.rsc.org/?DOI=C8LC01320A
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http://dx.doi.org/10.1039/c8lc01320aDOI Listing
February 2019
2 Reads

On-chip stool liquefaction via acoustofluidics.

Lab Chip 2019 Jan 31. Epub 2019 Jan 31.

Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.

Microfluidic-based portable devices for stool analysis are important for detecting established biomarkers for gastrointestinal disorders and understanding the relationship between gut microbiota imbalances and various health conditions, ranging from digestive disorders to neurodegenerative diseases. However, the challenge of processing stool samples in microfluidic devices hinders the development of a standalone platform. Here, we present the first microfluidic chip that can liquefy stool samples via acoustic streaming. Read More

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http://dx.doi.org/10.1039/c8lc01310aDOI Listing
January 2019

A microfluidic platform for the characterisation of membrane active antimicrobials.

Lab Chip 2019 Jan 30. Epub 2019 Jan 30.

Cavendish Laboratory, Univ. of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK.

The spread of bacterial resistance against conventional antibiotics generates a great need for the discovery of novel antimicrobials. Polypeptide antibiotics constitute a promising class of antimicrobial agents that favour attack on bacterial membranes. However, efficient measurement platforms for evaluating their mechanisms of action in a systematic manner are lacking. Read More

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http://xlink.rsc.org/?DOI=C8LC00932E
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http://dx.doi.org/10.1039/c8lc00932eDOI Listing
January 2019
3 Reads

Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy.

Lab Chip 2019 Jan 30. Epub 2019 Jan 30.

Department of Bioengineering, McGill University, Montreal, Quebec H3A 0E9, Canada. and Department of Pathology and Laboratory Medicine, University of California Davis, Davis, CA, USA.

In this paper we present for the first time a system comprised of two mobile phones, one for illumination and the other for microscopy, as a portable, user-friendly, and cost-effective microscopy platform for a wide range of applications. Versatile and adaptive illumination is made with a Retina display of an Apple mobile phone device. The phone screen is used to project various illumination patterns onto the specimen being imaged, each corresponding to a different illumination mode, such as bright-field, dark-field, point illumination, Rheinberg illumination, and fluorescence microscopy. Read More

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http://dx.doi.org/10.1039/c8lc00995cDOI Listing
January 2019
1 Read

Rapid liquid biopsy for Mohs surgery: rare target cell separation from surgical margin lavage fluid with a high recovery rate and selectivity.

Lab Chip 2019 Jan 29. Epub 2019 Jan 29.

Institute of Microelectronics, Peking University, Beijing 100871, China. and National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing 100871, China.

In melanoma surgery, it is difficult to identify residual scattered tumor cells at the surgical margin because of invasive growth. Mohs surgery, widely applied to increase the cure rate and decrease the recurrence rate of melanoma, involves examination of the tissue for tumor cells after tissue removal. Here, we established a liquid biopsy platform for rapid (<5 h), sensitive examination of residual tumor cells at the margin after Mohs surgery using clinical samples from patients with pigment nevus for a demonstration. Read More

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http://xlink.rsc.org/?DOI=C8LC01335G
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http://dx.doi.org/10.1039/c8lc01335gDOI Listing
January 2019
3 Reads

Live sperm trap microarray for high throughput imaging and analysis.

Lab Chip 2019 Jan 29. Epub 2019 Jan 29.

Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, ON M5S 3G8, Canada.

There is a growing appreciation and understanding of cell-to-cell variability in biological samples. However, research and clinical practice in male fertility has relied on population, or sample-based characteristics. Single-cell resolution is particularly important given the winner-takes-all nature of both natural and in vitro fertilization: it is the properties of a single cell, not the population, that are passed to the next generation. Read More

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http://dx.doi.org/10.1039/c8lc01204kDOI Listing
January 2019
1 Read

Chloroform compatible, thiol-ene based replica molded micro chemical devices as an alternative to glass microfluidic chips.

Lab Chip 2019 Jan 28. Epub 2019 Jan 28.

Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark.

Polymeric microfluidic chips offer a number of benefits compared to their glass equivalents, including lower material costs and ease and flexibility of fabrication. However, the main drawback of polymeric materials is often their limited resistance to (organic) solvents. Previously, thiol-ene materials were shown to be more solvent resistant than most other commonly used polymers; however, they still fall short in "harsh" chemical environments, such as when chlorinated solvents are present. Read More

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http://dx.doi.org/10.1039/c8lc01260aDOI Listing
January 2019
1 Read

Liquid biopsy: a perspective for probing blood for cancer.

Lab Chip 2019 Feb;19(4):548-549

Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA.

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http://xlink.rsc.org/?DOI=C8LC90117A
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http://dx.doi.org/10.1039/c8lc90117aDOI Listing
February 2019
3 Reads

Storing and releasing rhodamine as a model hydrophobic compound in polydimethylsiloxane microfluidic devices.

Lab Chip 2019 Feb;19(4):574-579

Dept of Bioengineering, University of Illinois at Chicago, Chicago, USA.

Polydimethylsiloxane (PDMS) is a ubiquitous material used in soft lithography and microfluidics. Due to its hydrophobic nature, PDMS tends to absorb small hydrophobic molecules, and is seen as a major disadvantage of the material in pharmaceutical and cell culture studies. While there have been extensive reports of attempts to treat PDMS to limit or block this absorption, little attention has been given to using this property as a feature in microfluidic devices. Read More

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http://xlink.rsc.org/?DOI=C9LC00039A
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http://dx.doi.org/10.1039/c9lc00039aDOI Listing
February 2019
1 Read

A liquid metal based capacitive soft pressure microsensor.

Lab Chip 2019 Jan 25. Epub 2019 Jan 25.

Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. and University of Chinese Academy of Sciences, Beijing 100039, China.

A liquid-metal based capacitive soft pressure microsensor is proposed in this work for measuring pressure in microchannels. To measure the pressure of the target microchannel, a short detection channel is fabricated and connected to the target microchannel. Because the detection channel has only one outlet at the end which is connected to the target microchannel, the fluid in the detection channel will stay still during the measurement and the pressure remains constant inside the detection channel. Read More

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http://dx.doi.org/10.1039/c8lc01357hDOI Listing
January 2019

Analysis and simulation of multiphase hydrodynamics in capillary microseparators.

Lab Chip 2019 Feb;19(4):706-715

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

The capillary microseparator is an important microfluidic device for achieving the inline separation of biphasic segmented flows. While it has found wide applications in areas such as on-chip synthesis of pharmaceuticals and fine chemicals, many aspects regarding its operating ranges and hydrodynamic details remain to be elucidated. In this work, we employ OpenFOAM computational fluid dynamics (CFD) method to systematically simulate the performance of the capillary microseparator under the retention, normal operation and breakthrough regimes. Read More

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http://dx.doi.org/10.1039/c8lc01296bDOI Listing
February 2019
9 Reads

Single-step assembly of asymmetric vesicles.

Lab Chip 2019 Jan 23. Epub 2019 Jan 23.

School of Engineering and Applied Science and Department of Physics, Harvard University, 02138 Cambridge, MA, USA.

Asymmetric vesicles are membranes in which amphiphiles are asymmetrically distributed between each membrane leaflet. This asymmetry dictates chemical and physical properties of these vesicles, enabling their use as more realistic models of biological cell membranes, which also are asymmetric, and improves their potential for drug delivery and cosmetic applications. However, their fabrication is difficult as the self-assembly of amphiphiles always leads to symmetric vesicles. Read More

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http://dx.doi.org/10.1039/c8lc00882eDOI Listing
January 2019

Paraffin-embedding lithography and micro-dissected tissue micro-arrays: tools for biological and pharmacological analysis of ex vivo solid tumors.

Lab Chip 2019 Feb;19(4):693-705

Centre de recherche du CHUM (CRCHUM)/Institut du Cancer de Montréal, Montreal, Quebec, Canada.

There is an urgent need and strong clinical and pharmaceutical interest in developing assays that allow for the direct testing of therapeutic agents on primary tissues. Current technologies fail to provide the required sample longevity, throughput, and integration with standard clinically proven assays to make the approach viable. Here we report a microfluidic micro-histological platform that enables ex vivo culture of a large array of prostate and ovarian cancer micro-dissected tissue (MDT) followed by direct on-chip fixation and paraffination, a process we term paraffin-embedding lithography (PEL). Read More

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http://dx.doi.org/10.1039/c8lc00982aDOI Listing
February 2019

Spatiotemporal quantification of acoustic cell patterning using Voronoï tessellation.

Lab Chip 2019 Feb;19(4):562-573

Department of Materials, Department of Bioengineering, and Institute for Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.

Acoustic patterning using ultrasound standing waves has recently emerged as a potent biotechnology enabling the remote generation of ordered cell systems. This capability has opened up exciting opportunities, for example, in guiding the development of organoid cultures or the organization of complex tissues. The success of these studies is often contingent on the formation of tightly-packed and uniform cell arrays; however, a number of factors can act to disrupt or prevent acoustic patterning. Read More

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http://dx.doi.org/10.1039/c8lc01108gDOI Listing
February 2019
2 Reads

Three-dimensional origami paper-based device for portable immunoassay applications.

Lab Chip 2019 Feb;19(4):598-607

Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan.

In this study, we demonstrate a three-dimensional surface-modified origami-paper-based analytical device (3D-soPAD) for immunoassay applications. The platform enables the sequential steps of immunoassays to be easily performed using a folded, sliding paper design featuring multiple pre-stored reagents, allowing us to take advantage of the vertical diffusion of the analyte through the different paper layers. The cellulose substrate is composed of carboxymethyl cellulose modified with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, which provide covalent bonding sites for bio-recognition molecules. Read More

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http://dx.doi.org/10.1039/c8lc01255eDOI Listing
February 2019

Introducing an automated high content confocal imaging approach for Organs-on-Chips.

Lab Chip 2019 Jan;19(3):410-421

AstraZeneca IMED Biotech Unit, Discovery Sciences, Cambridge, UK.

Organ-Chips are micro-engineered systems that aim to recapitulate the organ microenvironment. Implementation of Organ-Chips within the pharmaceutical industry aims to improve the probability of success of drugs reaching late stage clinical trial by generating models for drug discovery that are of human origin and have disease relevance. We are adopting the use of Organ-Chips for enhancing pre-clinical efficacy and toxicity evaluation and prediction. Read More

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http://xlink.rsc.org/?DOI=C8LC00829A
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http://dx.doi.org/10.1039/c8lc00829aDOI Listing
January 2019
6 Reads

Latchable microfluidic valve arrays based on shape memory polymer actuators.

Lab Chip 2019 Feb;19(4):608-617

Soft Transducers Laboratory (LMTS), Ecole Polytechnique Fédérale de Lausanne (EPFL), 2000 Neuchâtel, Switzerland.

We report arrays of latching microfluidic valves based on shape memory polymers (SMPs), and show their applications as reagent mixers and as peristaltic pumps. The valve design takes advantage of the SMP's multiple stable shapes and over a hundred-fold stiffness change with temperature to enable a) permanent zero-power latching in either open or closed positions (>15 h), as well as b) extended cyclic operation (>3000 cycles). The moving element in the valves consists of a tri-layer with a 50 μm thick central SMP layer, 25 μm thick patterned carbon-silicone (CB/PDMS) heaters underneath, and a 38 μm thick styrene ethylene butylene styrene (SEBS) impermeable film on top. Read More

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http://dx.doi.org/10.1039/c8lc01024bDOI Listing
February 2019

Femtoliter droplet confinement of Streptococcus pneumoniae: bacterial genetic transformation by cell-cell interaction in droplets.

Lab Chip 2019 Feb;19(4):682-692

Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.

Streptococcus pneumoniae (pneumococcus), a deadly bacterial human pathogen, uses genetic transformation to gain antibiotic resistance. Genetic transformation begins when a pneumococcal strain in a transient specialized physiological state called competence, attacks and lyses another strain, releasing DNA, taking up fragments of the liberated DNA, and integrating divergent genes into its genome. While many steps of the process are known and generally understood, the precise mechanism of this natural genetic transformation is not fully understood and the current standard strategies to study it have limitations in specifically controlling and observing the process in detail. Read More

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http://dx.doi.org/10.1039/c8lc01367eDOI Listing
February 2019

SERS-based droplet microfluidics for high-throughput gradient analysis.

Lab Chip 2019 Feb;19(4):674-681

Department of Bionano Technology, Hanyang University, Ansan 15588, South Korea.

In the last two decades, microfluidic technology has emerged as a highly efficient tool for the study of various chemical and biological reactions. Recently, we reported that high-throughput detection of various concentrations of a reagent is possible using a continuous gradient microfluidic channel combined with a surface-enhanced Raman scattering (SERS) detection platform. In this continuous flow regime, however, the deposition of nanoparticle aggregates on channel surfaces induces the "memory effect," affecting both sensitivity and reproducibility. Read More

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http://xlink.rsc.org/?DOI=C8LC01180J
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http://dx.doi.org/10.1039/c8lc01180jDOI Listing
February 2019
5 Reads

Large-scale production of compound bubbles using parallelized microfluidics for efficient extraction of metal ions.

Lab Chip 2019 Feb;19(4):665-673

Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Recent advances in microfluidic technologies have enabled production of micro-scale compound bubbles that consist of gaseous cores surrounded by thin liquid shells, achieving control and uniformity not possible using conventional techniques. These compound bubbles have demonstrated enormous utility as functional materials for drug delivery, as ultra-lightweight structural materials, as engineered acoustic materials, and also as separating agents for extraction of metal ions from waste fluid streams. Despite these successful demonstrations, compound bubbles have largely remained at the laboratory-scale due to the slow production rates endemic to microfluidics (<10 mL h-1). Read More

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http://dx.doi.org/10.1039/c8lc01267aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372331PMC
February 2019
1 Read

A miniaturized optical tomography platform for volumetric imaging of engineered living systems.

Lab Chip 2019 Feb;19(4):550-561

Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne St, Cambridge, MA 02139, USA.

Volumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications. The unprecedented structural details that these techniques provide have helped in our understanding of different aspects of architecture of cells, tissues, and organ systems as they occur in their natural states. Nonetheless, the instrumentation for most of these techniques is sophisticated, bulky, and costly, and is less affordable to most laboratory settings. Read More

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http://dx.doi.org/10.1039/c8lc01190gDOI Listing
February 2019

Two-level submicron high porosity membranes (2LHPM) for the capture and release of white blood cells (WBCs).

Lab Chip 2019 Feb;19(4):589-597

National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, Québec J4B 6Y4, Canada.

A method modifying a vacuum-assisted UV micro-molding (VAUM) process is proposed for the fabrication of polymer two-level submicron high porosity membranes (2LHPM). The modified process allows for the fabrication of robust, large-area membranes over 5 × 5 cm2 with a hierarchical architecture made from a 200 nm-thick layer having submicron level pores (as small as 500 nm) supported by a 20 μm-thick layer forming a microporous structure with 10-15 μm diameter pores. The fabricated freestanding membranes are flexible and mechanically robust enough for post manipulation and filtration of cell samples. Read More

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http://dx.doi.org/10.1039/c8lc01256cDOI Listing
February 2019

Organic-free, versatile sessile droplet microfluidic device for chemical separation using an aqueous two-phase system.

Lab Chip 2019 Feb;19(4):654-664

Department of Chemistry, Queen's University, 90 Bader Lane, Kingston, Ontario K7L 3N6, Canada.

This work presents a novel portable, versatile sessile droplet microfluidic (SDMF) device to perform liquid manipulation operations such as confining, splitting and colorimetric detection. Furthermore, chemical isolations based on an aqueous two-phase system (ATPS) for separating an analyte of choice from a complicated sample matrix can be carried out. ATPS extractions can replace conventional liquid-liquid extractions and take away the need for harmful organic solvents. Read More

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http://dx.doi.org/10.1039/c8lc01121dDOI Listing
February 2019

Digital microfluidics and nuclear magnetic resonance spectroscopy for in situ diffusion measurements and reaction monitoring.

Lab Chip 2019 Feb;19(4):641-653

Department of Chemistry, University of Toronto, 80 St George St., Toronto, ON M5S 3H6, Canada.

In recent years microcoils and related structures have been developed to increase the mass sensitivity of nuclear magnetic resonance spectroscopy, allowing this extremely powerful analytical technique to be extended to small sample volumes (<5 μl). In general, microchannels have been used to deliver the samples of interest to these microcoils; however, these systems tend to have large dead volumes and require more complex fluidic connections. Here, we introduce a two-plate digital microfluidic (DMF) strategy to interface small-volume samples with NMR microcoils. Read More

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http://dx.doi.org/10.1039/c8lc01214hDOI Listing
February 2019
1 Read