40,681 results match your criteria Klebsiella Infections


Genetic Alterations Associated with Polymyxin B Resistance in Nosocomial KPC-2-Producing from Brazil.

Microb Drug Resist 2021 Jun 15. Epub 2021 Jun 15.

Laboratório de Microbiologia Molecular, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brazil.

The rapid increased multidrug resistance in has led to a renewed interest in polymyxin antibiotics, such as colistin, as antibiotics of last resort, not least in low/middle income countries. We conducted a genomic survey of clinical polymyxin-resistant to investigate the genetic alterations in isolates harboring . Whole-genome sequencing was performed using an Illumina NextSeq 500 paired-end reads. Read More

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Insights into evolution and coexistence of the colibactin- and yersiniabactin secondary metabolite determinants in enterobacterial populations.

Microb Genom 2021 Jun;7(6)

Institute of Hygiene, University of Münster, Münster, Germany.

The bacterial genotoxin colibactin interferes with the eukaryotic cell cycle by causing dsDNA breaks. It has been linked to bacterially induced colorectal cancer in humans. Colibactin is encoded by a 54 kb genomic region in . Read More

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Predicting Antimicrobial Resistance Using Partial Genome Alignments.

mSystems 2021 Jun 15:e0018521. Epub 2021 Jun 15.

Consortium for Advanced Science and Engineering, University of Chicago, Chicago, Illinois, USA.

Antimicrobial resistance (AMR) is an important global health threat that impacts millions of people worldwide each year. Developing methods that can detect and predict AMR phenotypes can help to mitigate the spread of AMR by informing clinical decision making and appropriate mitigation strategies. Many bioinformatic methods have been developed for predicting AMR phenotypes from whole-genome sequences and AMR genes, but recent studies have indicated that predictions can be made from incomplete genome sequence data. Read More

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Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.

World J Microbiol Biotechnol 2021 Jun 15;37(7):117. Epub 2021 Jun 15.

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.

3-Hydroxypropionic acid (3-HP) represents an economically important platform compound from which a panel of bulk chemicals can be derived. Compared with petroleum-dependent chemical synthesis, bioproduction of 3-HP has attracted more attention due to utilization of renewable biomass. This review outlines bacterial production of 3-HP, covering aspects of host strains (e. Read More

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Burden of illness in US hospitals due to carbapenem-resistant Gram-negative urinary tract infections in patients with or without bacteraemia.

BMC Infect Dis 2021 Jun 14;21(1):572. Epub 2021 Jun 14.

Shionogi Inc., 300 Campus Drive, Suite 100, Florham Park, NJ, 07932, USA.

Background: Urinary tract infections (UTIs) are the most common infections caused by Gram-negative bacteria and represent a major healthcare burden. Carbapenem-resistant (CR) strains of Enterobacterales and non-lactose fermenting pathogens further complicate treatment approaches.

Methods: We conducted a retrospective analysis of the US Premier Healthcare Database (2014-2019) in hospitalised adults with a UTI to estimate the healthcare burden of Gram-negative CR UTIs among patients with or without concurrent bacteraemia. Read More

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Synthetic studies of cystobactamids as antibiotics and bacterial imaging carriers lead to compounds with high efficacy.

Chem Sci 2019 Dec 10;11(5):1316-1334. Epub 2019 Dec 10.

Department of Chemical Biology, Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany

There is an alarming scarcity of novel chemical matter with bioactivity against multidrug-resistant Gram-negative bacterial pathogens. Cystobactamids, recently discovered natural products from myxobacteria, are an exception to this trend. Their unusual chemical structure, composed of oligomeric -aminobenzoic acid moieties, is associated with a high antibiotic activity through the inhibition of gyrase. Read More

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December 2019

Antibody-recruiting protein-catalyzed capture agents to combat antibiotic-resistant bacteria.

Chem Sci 2020 Feb 12;11(11):3054-3067. Epub 2020 Feb 12.

Institute for Systems Biology 401 Terry Ave North Seattle 98109 USA

Antibiotic resistant infections are projected to cause over 10 million deaths by 2050, yet the development of new antibiotics has slowed. This points to an urgent need for methodologies for the rapid development of antibiotics against emerging drug resistant pathogens. We report on a generalizable combined computational and synthetic approach, called antibody-recruiting protein-catalyzed capture agents (AR-PCCs), to address this challenge. Read More

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February 2020

Rapid detection of human origin colistin-resistance genes mcr-1, mcr-3, mcr-8, mcr-10 in clinical fecal samples.

Arch Microbiol 2021 Jun 14. Epub 2021 Jun 14.

Key Laboratory of Molecular Epidemiology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Guangdong Province, No. 8 Longyuan Road, Nanshan district, Shenzhen City, 518055, People's Republic of China.

Plasmid-mediated colistin-resistance genes have been reported in human origin clinical samples worldwide which raises its threats to human infections. Notably, mcr-1, mcr-3, mcr-8, and mcr-10 have been reported isolated directly from clinical samples which creates more seriously threaten to human health than other mcr gene types. A multiplex polymerase chain reaction (Multi-PCR) protocol was developed to detect and genotype mobile colistin-resistance genes (mcr-1, mcr-3, mcr-8, mcr-10) in Enterobacteria for clinical laboratory purposes. Read More

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Plasma Microbial Cell-Free DNA Sequencing Technology for the Diagnosis of Sepsis in the ICU.

Front Mol Biosci 2021 28;8:659390. Epub 2021 May 28.

Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Sepsis is a common life-threatening disease in the intensive care unit (ICU) that is usually treated empirically without pathogen identification. As a non-invasive and high-throughput technology, plasma microbial cell-free DNA (mcfDNA) sequencing can detect unknown pathogens independent of previous clinical or laboratory information. In this study, a total of 199 cases suspected of bloodstream infection (BSI) from January 2020 to June 2020 were collected, and potential pathogens were detected by simultaneous blood culture and plasma mcfDNA sequencing. Read More

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A retrospective study on the microbial spectrum and antibiogram of uropathogens in children in a secondary care hospital in Rural Vellore, South India.

J Family Med Prim Care 2021 Apr 29;10(4):1706-1711. Epub 2021 Apr 29.

Department of Community Health, Christian Medical College, Vellore, Tamil Nadu, India.

Background: Urinary tract infection (UTI) is common among children. Empiric antibiotics have to be started as early as possible or it may lead to an irreversible renal parenchymal damage and renal scarring in children. The objectives were to determine the prevalence and microbial profile of paediatric UTI and to determine the antimicrobial susceptibility pattern. Read More

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Genetic Determinants of Resistance among ESBL-Producing in Community and Hospital Settings in East, Central, and Southern Africa: A Systematic Review and Meta-Analysis of Prevalence.

Can J Infect Dis Med Microbiol 2021 2;2021:5153237. Epub 2021 Jun 2.

Directorate of Information Technology and Communication, National Institute for Medical Research, P.O. Box 9653, Dar es Salaam, Tanzania.

Background: The world prevalence of community and hospital-acquired extended-spectrum lactamase (ESBL)-producing is increasing tremendously. Bacteria harboring ESBLs are currently the number one critical pathogens posing a major threat to human health.

Objective: To provide a summary of molecular evidence on the prevalence of ESBL-producing (ESBL-E) and associated genes at community and hospital settings in East, Central, and Southern African countries. Read More

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Transcriptome Analysis Reveals Cr(VI) Adaptation Mechanisms in sp. Strain AqSCr.

Front Microbiol 2021 27;12:656589. Epub 2021 May 27.

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.

sp. strain AqSCr, isolated from Cr(VI)-polluted groundwater, reduces Cr(VI) both aerobically and anaerobically and resists up 34 mM Cr(VI); this resistance is independent of the ChrA efflux transporter. In this study, we report the whole genome sequence and the transcriptional profile by RNA-Seq of strain AqSCr under Cr(VI)-adapted conditions and found 255 upregulated and 240 downregulated genes compared to controls without Cr(VI) supplementation. Read More

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Carbapenemases producing Klebsiella pneumoniae from the pus of hospitalized patients: In-vitro antibiotic properties of Streptomyces against multidrug resistant infectious bacteria.

J Infect Public Health 2021 May 27;14(7):892-897. Epub 2021 May 27.

Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.

Background: Klebsiella pneumoniae is predominantly exists in the pus of the human wounds and it creates massive infections in the skin and causes serious health associated infections. Modern antibiotics are highly active in the treatment of wound infections. In this study was aimed to determine resistance of K. Read More

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Importation of Entamoeba histolytica and predominance of Klebsiella pneumoniae in liver abscesses: a 7-year retrospective cohort study from the United Arab Emirates.

Trop Dis Travel Med Vaccines 2021 Jun 12;7(1):17. Epub 2021 Jun 12.

Department of Internal Medicine, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.

Background: There is a dearth of information on liver abscesses in the United Arab Emirates. Herein, we describe the clinical features of liver abscesses and determine their incidence rates and clinical outcomes.

Methods: We retrospectively reviewed the clinical charts of adult patients with a primary diagnosis of liver abscess at a major hospital over a 7-year period. Read More

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Antimicrobial susceptibility of gram-positive and gram-negative bacteria: a 5-year retrospective analysis at a multi-hospital healthcare system in Saudi Arabia.

Ann Clin Microbiol Antimicrob 2021 Jun 12;20(1):43. Epub 2021 Jun 12.

College of Medicine, Alfaisal University, Riyadh, Saudi Arabia.

Background: Studying time-related changes in susceptible pathogens causing healthcare-associated infections (HAIs) is vital in improving local antimicrobial and infection control practices.

Objectives: Describe susceptibility patterns to several antimicrobials in gram-positive and gram-negative pathogens isolated from patients causing HAIs at three private tertiary care hospitals in Saudi Arabia over a 5-year period.

Methods: Data on trends of antimicrobial susceptibility among bacteria causing HAIs events in children and adults at three tertiary private hospitals located in Riyadh and Qassim, Saudi Arabia, were collected retrospectively between 2015 and 2019 using the surveillance data datasets. Read More

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Global trends and hotspots in research of carbapenem-resistant Enterobacteriaceae (CRE): a bibliometric analysis from 2010 to 2020.

Ann Palliat Med 2021 May 25. Epub 2021 May 25.

Department of Critical Care Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Background: Infectious disease caused by carbapenem-resistant Enterobacteriaceae (CRE) has become one of the most serious challenges due to its high morbidity and mortality and research on it has aroused great concern worldwide in the last decade. Thus, a bibliometric analysis of relevant publications is needed to identify the situation of current investigations and prioritize the future research areas.

Methods: The current study retrieved articles related to CRE published between 2010 and 2020 from the Web of Science core collection database. Read More

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Rapid detection of colistin-resistant Enterobacterales using the resazurin reduction-based assay.

J Glob Antimicrob Resist 2021 Jun 9. Epub 2021 Jun 9.

Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, 840212, Sultan Abubakar Road, City Campus Complex, Sokoto State, Nigeria.

Objective: The emergence and dissemination of resistance determinants that confer colistin resistance among the Enterobacterales isolated from foods, animals, and humans create a serious public health problem due to the lack of remedial choices for infections caused by these isolates. This research aimed to evaluate the potential application of the resazurin reduction-based Rapid ResaPolymyxin NP assay in detecting the susceptibility of colistin in Enterobacterales .

Methods: Colistinsusceptibility test was performed by Rapid ResaPolymyxin NP technique and compared with reference broth microdilution method (BMD). Read More

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Establishing a quantitative index of meropenem hydrolysis for the detection of KPC- and NDM-producing bacteria by MALDI-TOF MS.

J Microbiol Methods 2021 Jun 9;187:106268. Epub 2021 Jun 9.

Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil. Electronic address:

Background: Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS), commonly used for microorganism identification, can also be applied for the detection of carbapenemase-producing bacteria by the evaluation of carbapenem hydrolysis. Since KPC- and NDM-producing bacteria are related to high mortality rates, diagnostic assays for its detection are essential. The aim of this study was to develop and evaluate a method to establish a quantitative measure (hydrolysis index - HI) to detect meropenem hydrolysis by MLADI-TOF MS. Read More

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Starvation induces shrinkage of the bacterial cytoplasm.

Proc Natl Acad Sci U S A 2021 Jun;118(24)

Department of Biology, Washington University in St. Louis, St. Louis, MO 63130;

Environmental fluctuations are a common challenge for single-celled organisms; enteric bacteria such as experience dramatic changes in nutrient availability, pH, and temperature during their journey into and out of the host. While the effects of altered nutrient availability on gene expression and protein synthesis are well known, their impacts on cytoplasmic dynamics and cell morphology have been largely overlooked. Here, we discover that depletion of utilizable nutrients results in shrinkage of 's inner membrane from the cell wall. Read More

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Etiology and prevalence of ESBLs in adult community-onset urinary tract infections in East China: a prospective multicenter study.

J Infect 2021 Jun 8. Epub 2021 Jun 8.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, Zhejiang, China; Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Objectives: Community onset urinary tract infections (COUTIs) drew attention recently owing to their increased prevalence and associations with resistant pathogens. The study is aimed at investigating the etiology of COUTIs as well as prevalence and the related risk factors of extended-spectrum β-lactamase (ESBL) in COUTIs in China.

Methods: The prospective study was performed in nineteen hospitals during November 1, 2017 and August 31, 2019. Read More

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The interface between COVID-19 and bacterial healthcare-associated infections.

Authors:
Ronan F O'Toole

Clin Microbiol Infect 2021 Jun 7. Epub 2021 Jun 7.

Department of Pharmacy and Biomedical Sciences, School of Molecular Sciences, College of Science, Health and Engineering, La Trobe University, Victoria, Australia. Electronic address:

Background: A wide range of bacterial infections occur in COVID-19 patients, particularly, in those with severe coronaviral disease. Some of these are community acquired co-infections.

Objective: To review recent data which indicate the occurrence of hospital-onset bacterial infections, including with antibiotic-resistant isolates, in COVID-19 patients. Read More

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The association between bacteria colonizing the upper respiratory tract and lower respiratory tract infection in young children: a systematic review and meta-analysis.

Clin Microbiol Infect 2021 Jun 7. Epub 2021 Jun 7.

Division of Medical Microbiology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa; Division of Infection and Immunity, School of Biomedical Sciences, University of Western Australia, Perth, Australia. Electronic address:

Background: Bacteria colonizing the upper respiratory tract (URT) of young children play a key role in the pathogenesis of lower respiratory tract infection (LRTI).

Objectives: To systematically review the literature on the association between bacteria colonizing the URT and LRTI among young children.

Data Sources: MEDLINE, Academic Search Premier, Africa-Wide Information and CINAHL, Scopus and Web of Science. Read More

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Evaluation of the antioxidant, antibacterial, and antibiofilm activity of the sesquiterpene nerolidol.

Arch Microbiol 2021 Jun 10. Epub 2021 Jun 10.

Laboratório de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.

The aim of this study was to evaluate the antioxidant, antibacterial, and antibiofilm activities of nerolidol. The antioxidant activity of nerolidol was determined using the total antioxidant activity method. Antibacterial activity was performed using the microdilution method to determine the minimum inhibitory concentration (MIC) against seven standard strains of the ATCC and four bacterial clinical isolates with a resistance profile, following the Clinical and Laboratory Standards Institute (CLSI). Read More

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Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil.

Arch Microbiol 2021 Jun 10. Epub 2021 Jun 10.

Postgraduate Program in Environmental Technology, Universidade de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.

Multidrug-resistant (MDR) and hypervirulent Klebsiella pneumoniae (hvKp) clones have become a major threat to global public health. The clonal group 258 (CG258) is considered a high-risk CG and the K. pneumoniae strains belonging to it are often multi-resistant and to spread mainly in the hospital environment. Read More

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rMAP: the Rapid Microbial Analysis Pipeline for ESKAPE bacterial group whole-genome sequence data.

Microb Genom 2021 Jun;7(6)

The African Center of Excellence in Bioinformatics and Data-Intensive Sciences, the Infectious Diseases Institute, College of Health Sciences, Makerere University, Kampala, Uganda.

The recent re-emergence of multidrug-resistant pathogens has exacerbated their threat to worldwide public health. The evolution of the genomics era has led to the generation of huge volumes of sequencing data at an unprecedented rate due to the ever-reducing costs of whole-genome sequencing (WGS). We have developed the Rapid Microbial Analysis Pipeline (rMAP), a user-friendly pipeline capable of profiling the resistomes of ESKAPE pathogens (, , , , and species) using WGS data generated from Illumina's sequencing platforms. Read More

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Development of the infant gut microbiome predicts temperament across the first year of life.

Dev Psychopathol 2021 Jun 10:1-12. Epub 2021 Jun 10.

Department of Psychology, Chapman University, Orange, CA, USA.

Perturbations to the gut microbiome are implicated in altered neurodevelopmental trajectories that may shape life span risk for emotion dysregulation and affective disorders. However, the sensitive periods during which the microbiome may influence neurodevelopment remain understudied. We investigated relationships between gut microbiome composition across infancy and temperament at 12 months of age. Read More

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Risk factors for the development of neonatal sepsis in a neonatal intensive care unit of a tertiary care hospital of Nepal.

BMC Infect Dis 2021 Jun 9;21(1):546. Epub 2021 Jun 9.

Oxford University Clinical Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal.

Background: Sepsis is an overwhelming and life-threatening response to bacteria in bloodstream and a major cause of neonatal morbidity and mortality. Understanding the etiology and potential risk factors for neonatal sepsis is urgently required, particularly in low-income countries where burden of infection is high and its epidemiology is poorly understood.

Methods: A prospective observational cohort study was conducted between April 2016 and October 2017 in a level three NICU at a tertiary care hospital in Nepal to determine the bacterial etiology and potential risk factors for neonatal sepsis. Read More

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Prevalence and risk factors associated with multi-drug resistant organisms (MDRO) carriage among pediatric patients at the time of admission in a tertiary care hospital of a developing country. A cross-sectional study.

BMC Infect Dis 2021 Jun 9;21(1):547. Epub 2021 Jun 9.

Department of Pediatrics and Child Health, Aga Khan University Hospital, Karachi, Pakistan.

Background: The rise of Multidrug-resistant organisms (MDROs) poses a considerable burden on the healthcare systems, particularly in low-middle income countries like Pakistan. There is a scarcity of data on the carriage of MDRO particularly in the pediatrics population therefore, we aimed to determine MDRO carriage in pediatric patients at the time of admission to a tertiary care hospital in Karachi, Pakistan, and to identify the risk factors associated with it.

Methods: A cross-sectional study conducted at the pediatric department of Aga Khan University Hospital (AKUH) from May to September 2019 on 347 children aged 1-18 years. Read More

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The role of combination therapy in the treatment of severe infections caused by carbapenem resistant gram-negatives: a systematic review of clinical studies.

BMC Infect Dis 2021 Jun 9;21(1):545. Epub 2021 Jun 9.

Division of Infectious Diseases, Department of Diagnostic and Public Health, University of Verona, P.Le L.A. Scuro 10, 37134, Verona, Italy.

Background: Effective treatment of sepsis due to carbapenem-resistant Gram-negative bacteria (CR-GNB) remains a challenge for clinicians worldwide. In recent years, the combination of antibiotics has become the preferred treatment strategy for CR-GNB infection. However, robust evidence to support this approach is lacking. Read More

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