45 results match your criteria Kinetoplastid Biology and Disease [Journal]

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Consultation meeting on the development of therapeutic vaccines for post kala azar dermal leishmaniasis.

Kinetoplastid Biol Dis 2007 Aug 17;6. Epub 2007 Aug 17.

Centre for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.

Background: Post kala azar dermal leishmaniasis (PKDL) is a disease that appears after treatment of visceral leishmaniasis (VL). The highest incidence of PKDL in the world is in Sudan. Many patients heal spontaneously within 6 months but those who don't are difficult to treat, often requiring months of daily injections. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-6-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2000869PMC
August 2007
7 Reads

In silico, biologically-inspired modelling of genomic variation generation in surface proteins of Trypanosoma cruzi.

Kinetoplastid Biol Dis 2007 Jul 10;6. Epub 2007 Jul 10.

Computer Science Research Institute and School of Computing and Mathematics, University of Ulster, Jordanstown, BT37 OQB, Northern Ireland, UK.

Background: Protozoan parasites improve the likelihood of invading or adapting to the host through their capacity to present a large repertoire of surface molecules. The understanding of the mechanisms underlying the generation of antigenic diversity is crucial to aid in the development of therapies and the study of evolution. Despite advances driven by molecular biology and genomics, there is a need to gain a deeper understanding of key properties that may facilitate variation generation, models for explaining the role of genomic re-arrangements and the characterisation of surface protein families on the basis of their capacity to generate variation. Read More

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http://dx.doi.org/10.1186/1475-9292-6-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1965468PMC
July 2007
5 Reads

Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping.

Kinetoplastid Biol Dis 2007 Jun 21;6. Epub 2007 Jun 21.

Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121, 40296-710, Salvador, BA, Brazil.

Background: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-6-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1919383PMC
June 2007
5 Reads

Dynamics of infection and competition between two strains of Trypanosoma brucei brucei in the tsetse fly observed using fluorescent markers.

Kinetoplastid Biol Dis 2007 Jun 6;6. Epub 2007 Jun 6.

School of Biological Sciences University of Bristol, Bristol BS8 1UG, UK.

Background: Genetic exchange occurs between Trypanosoma brucei strains during the complex developmental cycle in the tsetse vector, probably within the salivary glands. Successful mating will depend on the dynamics of co-infection with multiple strains, particularly if intraspecific competition occurs. We have previously used T. Read More

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http://dx.doi.org/10.1186/1475-9292-6-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1899512PMC
June 2007
8 Reads

Consultative meeting to develop a strategy for treatment of cutaneous leishmaniasis. Institute Pasteur, Paris. 13-15 June, 2006.

Kinetoplastid Biol Dis 2007 Apr 24;6. Epub 2007 Apr 24.

Drugs for Neglected Diseases Initiative, 1 Place St Gervais, Geneva CH 1201, Switzerland.

Background: A meeting was organized by Drugs for Neglected Diseases initiative (DNDi) and the Institute Pasteur (IP), Paris, to review the treatment for all forms of cutaneous leishmaniasis (CL) and to propose a strategy for the development of new efficacious and affordable treatments.

Method: The global burden of CL was discussed with respect to financial impact; relation to poverty; the stigma of CL lesions and scars (particularly in young women); lack of effective, affordable, easily implemented tools and political will and resources to implement available control tools; and lack of input from pharmaceutical and biotechnology companies to develop new drugs and vaccines.

Results: According to the experts from different endemic countries present, the financial and social burdens of CL are high, but we have limited quantitative data. Read More

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http://dx.doi.org/10.1186/1475-9292-6-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868746PMC
April 2007
8 Reads

The cysteine proteinase inhibitor Z-Phe-Ala-CHN2 alters cell morphology and cell division activity of Trypanosoma brucei bloodstream forms in vivo.

Kinetoplastid Biol Dis 2007 Feb 28;6. Epub 2007 Feb 28.

Abteilung Parasitologie, Hygiene-Institut der Ruprecht Karls-Universität, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany.

Background: Current chemotherapy of human African trypanosomiasis or sleeping sickness relies on drugs developed decades ago, some of which show toxic side effects. One promising line of research towards the development of novel anti-trypanosomal drugs are small-molecule inhibitors of Trypanosoma brucei cysteine proteinases.

Results: In this study, we demonstrate that treatment of T. Read More

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http://dx.doi.org/10.1186/1475-9292-6-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1810305PMC
February 2007
7 Reads

A method for the identification of guinea pig blood meal in the Chagas disease vector, Triatoma infestans.

Kinetoplastid Biol Dis 2007 Jan 12;6. Epub 2007 Jan 12.

Department of Biology, University of Vermont, 109 Carrigan Drive, Burlington, VT 04505, USA.

Background: In a SINE-based PCR assay, a primer set specific for guinea pig genome short interspersed elements DNA was used to test the utility of genomic markers for identifying the source of vertebrate blood meals of Triatoma infestans.

Methods: The investigation consisted of two assays. In Assay 1, thirty-six insects, collected from the Province of Zudáñez in Chuquisaca, Bolivia were frozen 1-40 hours after feeding, under controlled conditions, on guinea pigs. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-6-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783661PMC
January 2007
5 Reads

Leishmania amastigotes as targets for drug screening.

Kinetoplastid Biol Dis 2006 Oct 23;5. Epub 2006 Oct 23.

IRD, UR008 Pathogènie des Trypanosomatidés, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.

Direct drug screening against the mammalian stage of Leishmania has been hampered by cost and the time consuming effort required to accomplish it. The ability to derive transgenic Leishmania expressing reporter genes opened up new possibilities for the development of drug screening tests. Further developments to standardize and gather multiple informations could now be envisionned. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-5-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635419PMC
October 2006
6 Reads

Radically different maxicircle classes within the same kinetoplast: an artefact or a novel feature of the kinetoplast genome?

Kinetoplastid Biol Dis 2006 Sep 18;5. Epub 2006 Sep 18.

Department of Molecular Biology, Lomonosov Moscow State University, Vorobjevy Gory 1, Build. 12, 119992 Moscow, Russia.

We discuss here some results which suggest that radically different maxicircle classes coexist within the same kinetoplast. These data, although tentative and incomplete, may provide a new outlook on the kinetoplast genome structure and expression. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-5-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1578572PMC
September 2006
8 Reads

Highlights of the XXI annual meeting of the Brazilian Society of Protozoology, the XXXII annual meeting on Basic Research in Chagas' disease & an international symposium on vesicle trafficking in parasitic Protozoa--7 to 9 November 2005, Caxambu, Minas Gerais, Brazil.

Kinetoplastid Biol Dis 2006 Aug 17;5. Epub 2006 Aug 17.

Departamento de Microbiologia, Imunologia e Parasitologia and Centro Interdisciplinar de Terapia Gênica (CINTERGEN), R. Mirassol, 207, 04044-010, São Paulo, Brazil.

This report focuses on the 2005 Annual meeting held in Caxambu, Minas Gerais, Brazil that was convened and organized by the Brazilian Society of Protozoology http://www.sbpz.org. Read More

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http://dx.doi.org/10.1186/1475-9292-5-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1560148PMC
August 2006
10 Reads

In vitro growth inhibition of bloodstream forms of Trypanosoma brucei and Trypanosoma congolense by iron chelators.

Kinetoplastid Biol Dis 2006 Aug 16;5. Epub 2006 Aug 16.

Department of Parasitology, Ruprecht-Karls-University, 69120 Heidelberg, Germany.

African trypanosomes exert significant morbidity and mortality in man and livestock. Only a few drugs are available for the treatment of trypanosome infections and therefore, the development of new anti-trypanosomal agents is required. Previously it has been shown that bloodstream-form trypanosomes are sensitive to the iron chelator deferoxamine. Read More

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http://dx.doi.org/10.1186/1475-9292-5-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1563472PMC
August 2006
21 Reads

A simple and rapid method for detection of Trypanosoma evansi in the dromedary camel using a nested polymerase chain reaction.

Kinetoplastid Biol Dis 2006 May 20;5. Epub 2006 May 20.

Molecular Biology Research Unit, National Ribat University, Khartoum, Sudan.

A nested polymerase chain reaction (nPCR)-based assay, was developed and evaluated for rapid detection of Trypanosoma evansi in experimentally infected mice and naturally infected camels (Camelus dromedarius). Four oligonucleotide primers (TE1, TE2, TE3 and TE4), selected from nuclear repetitive gene of T. evansi, were designed and used for PCR amplifications. Read More

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http://dx.doi.org/10.1186/1475-9292-5-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1481541PMC
May 2006
4 Reads

A new initiative for the development of new diagnostic tests for human African trypanosomiasis.

Kinetoplastid Biol Dis 2006 Apr 25;5. Epub 2006 Apr 25.

Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK.

Human African trypanosomiasis is a threat to millions of people living in sub-Saharan countries and is fatal unless treated. At present, the serological and parasitological tests used in the field for diagnosis of sleeping sickness have low specificity and sensitivity. There is clearly an urgent need for accurate tools for both diagnosis and staging of the disease. Read More

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http://dx.doi.org/10.1186/1475-9292-5-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464096PMC
April 2006
5 Reads

LmxMPK4, a mitogen-activated protein (MAP) kinase homologue essential for promastigotes and amastigotes of Leishmania mexicana.

Kinetoplastid Biol Dis 2005 Dec 29;4. Epub 2005 Dec 29.

Bernhard Nocht Institute for Tropical Medicine, Parasitology Section, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany.

Background: Leishmania parasites undergo profound morphological and biochemical changes while passing through their life cycle. Protein kinases have been shown to be involved in the differentiation from the extracellular flagellated promastigotes to the intracellular "non-flagellated" amastigotes and vice versa. Moreover, these enzymes are likely involved in the regulation of the proliferation of the different life stages. Read More

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http://dx.doi.org/10.1186/1475-9292-4-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1327672PMC
December 2005
8 Reads

Detection of trypanosomes in small ruminants and pigs in western Kenya: important reservoirs in the epidemiology of sleeping sickness?

Kinetoplastid Biol Dis 2005 Jul 14;4. Epub 2005 Jul 14.

Department of Biochemistry and Biotechnology, Kenyatta University, 00100-Nairobi, Kenya.

Background: Trypanosomosis is a major impediment to livestock farming in sub-Saharan Africa and limits the full potential of agricultural development in the 36 countries where it is endemic. In man, sleeping sickness is fatal if untreated and causes severe morbidity. This study was undertaken in western Kenya, an area that is endemic for both human and livestock trypanosomosis. Read More

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http://dx.doi.org/10.1186/1475-9292-4-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1200563PMC
July 2005
8 Reads

Application of direct agglutination test (DAT) and fast agglutination screening test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil.

Kinetoplastid Biol Dis 2005 Jun 14;4. Epub 2005 Jun 14.

Universidade do Estado de Minas Gerais, Fundação Educacional de Divinópolis, Divinópolis, MG, Brazil.

Background: The direct agglutination test (DAT) has proved to be a very important sero-diagnostic tool combining high levels of intrinsic validity and ease of performance. Otherwise, fast agglutination screening test (FAST) utilises only one serum dilution making the test very suitable for the screening of large populations.

Results: We have tested FAST and DAT for the detection anti-Leishmania antibodies in serum samples from patients with American visceral (AVL) and cutaneous leishmaniases (ACL) in Minas Gerais State, Brazil. Read More

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http://dx.doi.org/10.1186/1475-9292-4-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183242PMC
June 2005
7 Reads

Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10.

Kinetoplastid Biol Dis 2005 Apr 29;4(1). Epub 2005 Apr 29.

Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht St, 74, D-20359 Hamburg, Germany.

BACKGROUND: Leishmania spp., in the course of their parasitic life cycle, encounter two vastly different environments: the gut of sandflies and the phagosomes of mammalian macrophages. During transmission into a mammal, the parasites are exposed to increased ambient temperature as well as to different carbon sources. Read More

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http://dx.doi.org/10.1186/1475-9292-4-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1097755PMC
April 2005
4 Reads

Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.

Kinetoplastid Biol Dis 2005 25;4. Epub 2005 Jan 25.

Laboratorio de Biología Molecular, Instituto de Biomedicina, Universidad de Central Venezuela, Apartado 4043, Caracas 1010A, Venezuela.

We evaluated the adoptive transfer of DCs on -infected neonatal BALB/c mice. DCs were isolated and purified from the spleens of the following donor groups: a) Adult BALB/c mice infected during adulthood with ; b) Adult BALB/c mice infected during neonatal life; c) Healthy neonatal BALB/c mice; d) Healthy adult BALB/c mice. A neonatal model of infection, generated after inoculation with 5 × 10 promastigotes of , was used as the infection control group. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-4-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC548294PMC
January 2005
4 Reads

Experimental study of the function of the excreted/secreted Leishmania LmSIR2 protein by heterologous expression in eukaryotic cell line.

Kinetoplastid Biol Dis 2005 Jan 24;4(1). Epub 2005 Jan 24.

UR008 "Pathogénie des Trypanosomatidés", Centre IRD de Montpellier, 911 avenue Agropolis, BP5045, 34032 Montpellier, France.

BACKGROUND: In yeast and Caenorhabditis elegans, Silent Information Regulator (SIR2) proteins have been shown to be involved in ageing regulation. In Leishmania, the LmSIR2rp was originally isolated from the excreted/secreted material of the Leishmania parasites. Among the function(s) of this protein in Leishmania biology, we have documented its implication in parasite survival, and in particular in Leishmania amastigotes. Read More

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http://kinetoplastids.biomedcentral.com/articles/10.1186/147
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http://dx.doi.org/10.1186/1475-9292-4-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC548286PMC
January 2005
7 Reads

Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections.

Kinetoplastid Biol Dis 2004 Sep 17;3(1). Epub 2004 Sep 17.

Faculty of Agriculture and Applied Biological Sciences, K, U, Leuven, Department of Animal Science, Kasteelpark Arenberg 30, 3000 Leuven, Belgium.

BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. Read More

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http://dx.doi.org/10.1186/1475-9292-3-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC521498PMC
September 2004
4 Reads

Trypanosoma rangeli Transcriptome Project: Generation and analysis of expressed sequence tags.

Kinetoplastid Biol Dis 2004 May 13;3(1). Epub 2004 May 13.

Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Caixa postal 476, Santa Catarina, Brazil, 88040-900.

Trypanosoma rangeli is an important hemoflagellate parasite of several mammalian species in Central and South America, sharing geographical areas, vectors and reservoirs with T. cruzi, the causative agent of Chagas disease. Thus, the occurrence of single and/or mixed infections, including in humans, must be expected and are of great importance for specific diagnosis and epidemiology. Read More

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http://dx.doi.org/10.1186/1475-9292-3-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC419976PMC
May 2004
6 Reads

Chagas' disease and AIDS.

Kinetoplastid Biol Dis 2004 May 13;3(1). Epub 2004 May 13.

Department of Medicine (Division of Infectious Diseases), Albert Einstein College of Medicine and Montefiore Medical Center, Bronx New York 10461, USA.

Chagas' disease caused by Trypanosoma cruzi is an opportunistic infection in the setting of HIV/AIDS. Some individuals with HIV and chronic T. cruzi infection may experience a reactivation, which is most commonly manifested by meningoencephalitis. Read More

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http://dx.doi.org/10.1186/1475-9292-3-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC420481PMC
May 2004
3 Reads

Species concepts for trypanosomes: from morphological to molecular definitions?

Authors:
Wendy Gibson

Kinetoplastid Biol Dis 2003 Oct 28;2(1):10. Epub 2003 Oct 28.

School of Biological Sciences, University of Bristol, Bristol BS8 1UG, UK.

The way species and subspecies names are applied in African trypanosomes of subgenera Trypanozoon and Nannomonas is reviewed in the light of data from molecular taxonomy. In subgenus Trypanozoon the taxonomic importance of pathogenicity, host range and distribution appear to have been inflated relative to actual levels of genetic divergence. The opposite is true for subgenus Nannomonas, where current taxonomic usage badly underrepresents genetic diversity. Read More

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http://dx.doi.org/10.1186/1475-9292-2-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC280663PMC
October 2003
2 Reads

Evolution of energy metabolism and its compartmentation in Kinetoplastida.

Kinetoplastid Biol Dis 2003 Oct 28;2(1):11. Epub 2003 Oct 28.

Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Université Catholique de Louvain, Avenue Hippocrate 74, B-1200 Brussels, Belgium.

Kinetoplastida are protozoan organisms that probably diverged early in evolution from other eukaryotes. They are characterized by a number of unique features with respect to their energy and carbohydrate metabolism. These organisms possess peculiar peroxisomes, called glycosomes, which play a central role in this metabolism; the organelles harbour enzymes of several catabolic and anabolic routes, including major parts of the glycolytic and pentosephosphate pathways. Read More

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http://dx.doi.org/10.1186/1475-9292-2-11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC317351PMC
October 2003
5 Reads

Genetic subdivisions within Trypanosoma cruzi (Discrete Typing Units) and their relevance for molecular epidemiology and experimental evolution.

Authors:
Michel Tibayrenc

Kinetoplastid Biol Dis 2003 Oct 28;2(1):12. Epub 2003 Oct 28.

UR62 "Genetics of Infectious Diseases", UMR CNRS/IRD 9926, IRD Centre, BP 64501, 34394 Montpellier Cedex 5, France.

BACKGROUND: This paper summarizes the main results obtained on Trypanosoma cruzi genetic diversity and population structure since this parasite became the theme of many genetic and molecular studies in the early seventies. RESULTS: T. cruzi exibits a paradigmatic pattern of long-term, clonal evolution, which has structured its natural populations into several discrete genetic subdivisions or "Discrete Typing Units" (DTU). Read More

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http://dx.doi.org/10.1186/1475-9292-2-12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270070PMC
October 2003
6 Reads

Concepts of species in trypanosomatids.

Authors:
Hooman Momen

Kinetoplastid Biol Dis 2003 Oct 28;2(1):13. Epub 2003 Oct 28.

World Health Organization EIP/IMD/BLT1211 Geneva 27, Switzerland.

This paper is a commentary on "Species concepts for trypanosomes: from morphological to molecular definitions?" by Wendy Gibson published in this journal 1. Taxonomy has been traditionally based on expert opinion which is influenced among other factors by the philosophical and educational background of the expert concerned. This has resulted in widely different criteria for species among the trypanosomatids when compared to the actual genetic diversity involved. Read More

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http://dx.doi.org/10.1186/1475-9292-2-13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270071PMC
October 2003
2 Reads

Physiological consequences of drug resistance in Leishmania and their relevance for chemotherapy.

Kinetoplastid Biol Dis 2003 Oct 28;2(1):14. Epub 2003 Oct 28.

Laboratory of Molecular Physiology, I,M,E, Facultad de Medicina, Universidad Central de Venezuela, Venezuela.

In the early twentieth century, infectious diseases were a leading cause of death worldwide. Through the following years, morbidity and mortality caused by infectious diseases decreased considerably in the developed world, but not in the developing world, where infectious diseases remain an important reason for concern. For example, leishmaniosis has become into a serious Third World problem. Read More

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http://dx.doi.org/10.1186/1475-9292-2-14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC272938PMC
October 2003
2 Reads

Molecular phylogenetics of Trypanosomatidae: contrasting results from 18S rRNA and protein phylogenies.

Kinetoplastid Biol Dis 2003 Oct 28;2(1):15. Epub 2003 Oct 28.

Department of Biological Sciences, University of South Carolina, Columbia SC 29205 USA.

Phylogenetic analyses of the family Trypanosomatidae have been conducted using both 18S rRNA gene sequences and a variety of protein sequences. Using a variety of phylogenetic methods, 18S rRNA phylogenies indicate that the genus Trypanosoma is not monophyletic. Rather, they suggest that the American and African trypanosomes constitute distinct clades. Read More

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http://dx.doi.org/10.1186/1475-9292-2-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270072PMC
October 2003
2 Reads

Towards a framework for the evolutionary genomics of Kinetoplastids: what kind of data and how much?

Kinetoplastid Biol Dis 2003 Oct 28;2(1):16. Epub 2003 Oct 28.

Lab, de Biologia Molecular de Tripanosomatídeos, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Av, Brasil 4365, Rio de Janeiro, RJ, Brasil, 21045-900.

The current status of kinetoplastids phylogeny and evolution is discussed in view of the recent progresses on genomics. Some ideas on a potential framework for the evolutionary genomics of kinetoplastids are presented. Read More

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http://dx.doi.org/10.1186/1475-9292-2-16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC280664PMC
October 2003
2 Reads

Chagas disease and HIV co-infection: genetic analyses of two Trypanosoma cruzi strains under experimental immunosuppression.

Kinetoplastid Biol Dis 2003 Oct 28;2(1):17. Epub 2003 Oct 28.

Departamento de Ciências Biológicas, Escola Nacional de Saúde Publica, Fundação Oswaldo Cruz-FIOCRUZ, Rua Leopoldo Bulhões, 1480/6 degrees andar, 21041-210, Rio de Janeiro, RJ, Brazil.

BACKGROUND: Recently new aspects of the immunopathology of Chagas disease have been described in patients infected with HIV and unusual clinical manifestations such as cutaneous lesions, involvement of central nervous system and/or serious cardiac lesions related to the reactivation of the parasite have been reported. Two uncloned Trypanosoma cruzi strains previously isolated from chronic chagasic patients with HIV co-infection were studied in order to evaluate the impact of the immunosuppression on the genetic diversity of the parasite. RESULTS: We have exploited an experimental model to determine whether genetically distinct populations appear after immunosuppression as a consequence of in vivo selection or in vitro propagation. Read More

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http://dx.doi.org/10.1186/1475-9292-2-17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270073PMC
October 2003
2 Reads

Evaluation of a polymerase chain reaction assay for the diagnosis of bovine trypanosomiasis and epidemiological surveillance in Bolivia.

Kinetoplastid Biol Dis 2003 Oct 28;2(1). Epub 2003 Oct 28.

Laboratorio de Investigación y Diagnóstico Veterinario (LIDIVET) Santa Cruz, Bolivia.

BACKGROUND: Sporadic outbreaks of bovine trypanosomiasis have been reported in Bolivia since 1996 when T. vivax and T. evansi were identified for the first time by parasitological means. Read More

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http://dx.doi.org/10.1186/1475-9292-2-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC280665PMC
October 2003
2 Reads

Life after death: are trypanosomatids programmed to die for the survival of their partners?

Kinetoplastid Biol Dis 2003 Jun 25;2(1). Epub 2003 Jun 25.

Institute of Biochemistry, University, of Lausanne, Ch, des Boveresses 155, 1066 Epalinges, Switzerland.

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http://dx.doi.org/10.1186/1475-9292-2-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC166280PMC
June 2003
3 Reads

Apoptosis-like death in trypanosomatids: search for putative pathways and genes involved.

Authors:
Ali Ouaissi

Kinetoplastid Biol Dis 2003 Jun 25;2(1). Epub 2003 Jun 25.

IRD UR 008 "Pathogénie des Trypanosomatides", Centre IRD de Montpellier, 911 Avenue Agropolis, BP 64501, 34394 Montpellier, France.

Members of the Trypanosomatidae family comprises species that are causative of important human diseases such as Chagas'disease, Leishmaniasis and sleeping sickness. A wealth of evidence has accumulated that illustrates the ability of these unicellular organisms to undergo, with or without induction (stress conditions), a cell death with some features resembling apoptosis-like phenomenon. However, despite the apparent phenotypic similarities between the apoptosis-like death of kinetoplastids and mammalian nucleated cell programmed cell death (PCD), the pathways seem to differ significantly. Read More

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http://dx.doi.org/10.1186/1475-9292-2-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC166162PMC
June 2003
3 Reads

Maintainence of parasitaemia - is it to die for?

Authors:
Kevin M Tyler

Kinetoplastid Biol Dis 2003 Jun 24;2(1). Epub 2003 Jun 24.

School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, Norfolk, UK.

One of the major differences between protozoan differentiation and metazoan differentiation is that protozoan cells normally retain potency during differentiation, which need not, therefore, be considered altruistic. Altruism does, however, arise at the level of the organism and consequently, protozoons have the potential to evolve altruistic traits. This is particularly true when, as with Trypanosoma brucei parasitaemias, populations are genetically homogeneous. Read More

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http://dx.doi.org/10.1186/1475-9292-2-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC166139PMC
June 2003
2 Reads

Programmed cell death in trypanosomatids: is it an altruistic mechanism for survival of the fittest?

Kinetoplastid Biol Dis 2003 Jun 25;2(1). Epub 2003 Jun 25.

Laboratory of Bacterial, Parasitic and Unconventional Agents,Division of Emerging and Transfusion Transmitted Diseases, OBRR/CBER/FDA, Bldg,29, Rm,222, HFM-310, 8800 Rockville Pike, Bethesda, MD 20892, USA.

The protozoan parasites Leishmania, Trypanosoma cruzi and Trypanosoma brucei show multiple features consistent with a form of programmed cell death (PCD). Despite some similarities with apoptosis of mammalian cells, PCD in trypanosomatid protozoans appears to be significantly different. In these unicellular organisms, PCD could represent an altruistic mechanism for the selection of cells, from the parasite population, that are fit to be transmitted to the next host. Read More

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http://dx.doi.org/10.1186/1475-9292-2-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC194864PMC
June 2003
3 Reads

Role of the long slender to short stumpy transition in the life cycle of the african trypanosomes.

Kinetoplastid Biol Dis 2003 Jun 25;2(1). Epub 2003 Jun 25.

Department of Epidemiology School of Public Health University of North Carolina Chapel Hill, North Carolina 27599-7435 U,S,A.

It is shown using mouse models that the African trypanosomes exert a significant drain upon their host's carbohydrate (energy) resources; and that the higher the parasitemia, the greater the energy demand. It is, therefore, hypothesized that the long slender (LS) to short stumpy (SS) transition evolved, in part, to help control the parasitemia and to increase host survival time. It is also suggested that the SS population is heterogeneous. Read More

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http://dx.doi.org/10.1186/1475-9292-2-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165594PMC
June 2003
2 Reads

The development of Trypanosoma brucei within the tsetse fly midgut observed using green fluorescent trypanosomes.

Kinetoplastid Biol Dis 2003 Apr 10;2(1). Epub 2003 Apr 10.

School of Biological Sciences, University of Bristol, Bristol BS8 1UG, UK.

BACKGROUND: The protozoan pathogen, Trypanosoma brucei, undergoes complex cycles of differentiation and multiplication in its vector, the tsetse fly, genus Glossina. Flies are refractory to infection and resistance mechanisms operate at a number of levels and timepoints. Here we have used highly conspicuous green fluorescent trypanosomes to study the early events in establishment of infection in the fly midgut. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC156611PMC
April 2003
7 Reads

Leishmania species and zymodemes isolated from endemic areas of cutaneous leishmaniasis in Jordan.

Kinetoplastid Biol Dis 2002 Nov 20;1(1). Epub 2002 Nov 20.

Department of Applied Biology, Jordan University of Science and Technology, P, O, Box 3030, Irbid 22110, Jordan.

BACKGROUND: Cutaneous leishmaniasis (CL) is endemic in the Middle Eastern countries. New cases are emerging in areas previously free of the disease. In Jordan, the diagnosis of cases during the 1960s and 1970s was mainly reported in military hospitals in Amman. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC149425PMC
November 2002
2 Reads

Molecular determinants and regulation of Leishmania virulence.

Kinetoplastid Biol Dis 2002 May 20;1(1). Epub 2002 May 20.

Department of Microbiology/Immunology, University of Health Sciences/Chicago Medical School, North Chicago, IL, USA.

A Leishmania model to explain microbial virulence in chronic infectious diseases is proposed. All these diseases progress from infection to symptomatic phase to host death or recovery. The outcome of each phase is depicted to result from the interactions of a distinct group of parasite molecules with a specific host immune compartment. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC119322PMC
May 2002
2 Reads

PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats.

Kinetoplastid Biol Dis 2002 May 29;1(1). Epub 2002 May 29.

CIRDES, BP454, 01 Bobo-Dioulasso, Burkina Faso.

Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas. T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC119323PMC
May 2002
2 Reads

From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

Kinetoplastid Biol Dis 2002 May 31;1(1). Epub 2002 May 31.

Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil.

Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC119324PMC
May 2002
3 Reads

What can we hope to gain for trypanosomiasis control from molecular studies on tsetse biology ?

Kinetoplastid Biol Dis 2002 Jun 6;1(1). Epub 2002 Jun 6.

Department of Epidemiology and Public Health, Section of Vector Biology, Yale University School of Medicine, 60 College St, 606 LEPH, New Haven, CT 06510, USA.

At times of crisis when epidemics rage and begin to take their toll on affected populations, as we have been witnessing with African trypanosomiasis in subSahara, the dichotomy of basic versus applied research deepens. While undoubtedly the treatment of thousands of infected people is the top priority, without continued research and development on the biology of disease agents and on ecological and evolutionary forces impacting these epidemics, little progress can be gained in the long run for the eventual control of these diseases. Here, we argue the need for additional research in one under-investigated area, that is the biology of the tsetse vector. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC119325PMC
June 2002
3 Reads

Salivaria or Stercoraria? The Trypanosoma rangeli dilemma.

Kinetoplastid Biol Dis 2002 Jul 5;1(1). Epub 2002 Jul 5.

Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Caixa, Postal 476, 88040-900 Florianópolis, SC, Brasil.

The taxonomic status of Trypanosoma rangeli as well as the tools for its molecular characterization is briefly commented. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC119326PMC
July 2002
68 Reads

Combating Kinetoplastid diseases.

Kinetoplastid Biol Dis 2002 Jul 5;1(1). Epub 2002 Jul 5.

Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Av, Brasil 4365, Rio de Janeiro, CEP 21045-900, Brasil.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC119327PMC
July 2002
5 Reads
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