4,878 results match your criteria Keratitis Herpes Simplex


Clinical Features and Management of Stenotrophomonas Maltophilia Keratitis.

Ophthalmol Ther 2021 May 13. Epub 2021 May 13.

Department of Ophthalmology, Chosun University College of Medicine, 365 Pilmun-daero, Dong-gu, Gwangju, Republic of Korea.

Introduction: Stenotrophomonas maltophilia keratitis is an uncommon infectious disease of the cornea. The clinical features, antibiotic susceptibility, and clinical outcomes of S. maltophilia keratitis were investigated in this study. Read More

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Combined Bacterial and Herpes Simplex Virus Keratitis following Small-Incision Lenticule Extraction for the Correction of Myopia.

Case Rep Ophthalmol 2021 Jan-Apr;12(1):227-231. Epub 2021 Apr 12.

Department of Cornea and Refractive Surgery, Instituto de Oftalmología, Conde de Valenciana, Mexico City, Mexico.

A 28-year-old male presented unilateral visual loss, intense ocular pain, redness and intraocular hypertension in his right eye 2 days after undergoing small-incision lenticule extraction (SMILE) in both eyes. Initial examination of the affected eye revealed the presence of white infiltrates within the corneal interface, as well as a central epithelial defect. The patient was diagnosed with infectious keratitis, posteriorly the eye was irrigated with balanced saline solution and treatment was initiated with hourly moxifloxacin 0. Read More

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Bilateral Limbal Stem Cell Alterations in Patients With Unilateral Herpes Simplex Keratitis and Herpes Zoster Ophthalmicus as Shown by In Vivo Confocal Microscopy.

Invest Ophthalmol Vis Sci 2021 May;62(6):12

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, No.143, Yiman Street, Harbin City, Nangang District, Heilongjiang Province, China.

Purpose: The purpose of this study was to investigate the limbal changes in the palisades of Vogt (POV) in patients with herpes simplex keratitis (HSK) and herpes zoster ophthalmicus (HZO) with the application of in vivo confocal microscopy (IVCM).

Methods: We enrolled 35 eyes of 35 consecutive patients with HSK and 4 patients with HZO in this observational study. Thirty-five participants were also recruited from a healthy population as the control group. Read More

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Subacute toxicological evaluation of AT-533 and AT-533 gel in Sprague-Dawley rats.

Exp Ther Med 2021 Jun 15;21(6):632. Epub 2021 Apr 15.

Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.

As a novel heat shock protein 90 inhibitor, AT-533 exhibits various biological activities , including anti-viral, anti-tumor and anti-inflammatory activities. Moreover, AT-533 gel, a gel dosage form of AT-533, has been suggested to have anti-keratitis and herpes simplex virus type-1 infection-induced effects on the skin lesions of animals. However, the safety evaluation of AT-533 and AT-533 gel has, to the best of our knowledge, not been examined in toxicological tests. Read More

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Herpes Simplex Keratitis in Patients with SARS-CoV-2 Infection: A Series of Five Cases.

Medicina (Kaunas) 2021 Apr 24;57(5). Epub 2021 Apr 24.

Department of Ophthalmology of Slovak Medical University and University Hospital in Bratislava, Antolska 11, 851 07 Bratislava, Slovakia.

Herpes simplex virus type 1 (HSV-1) is a leading cause of infectious blindness worldwide. Most of the initial infection cases manifest as acute epithelial keratitis. Reactivation of herpesviruses is common in critically ill patients, including patients with severe Coronavirus disease (COVID-19). Read More

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Commentary: Herpes simplex virus stromal keratitis preferred practice patterns among ophthalmologists vis-à-vis the Herpetic Eye Diseases Study.

Indian J Ophthalmol 2021 May;69(5):1340-1341

Department of Retina, Uvea, and Cataract, ASG Eye Hospital, 149 BT Road, Kolkata, West Bengal, India.

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Classification criteria for herpes simplex virus anterior uveitis.

Am J Ophthalmol 2021 Apr 9. Epub 2021 Apr 9.

the Department of Medicine, Texas A&M University, College Station, TX, USA.

Purpose: To determine classification criteria for herpes simplex virus (HSV) anterior uveitis DESIGN: : Machine learning of cases with HSV anterior uveitis and 8 other anterior uveitides.

Methods: Cases of anterior uveitides were collected in an informatics-designed preliminary database, and a final database was constructed of cases achieving supermajority agreement on the diagnosis, using formal consensus techniques. Cases were split into a training set and a validation set. Read More

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Polluted Air Exposure Compromises Corneal Immunity and Exacerbates Inflammation in Acute Herpes Simplex Keratitis.

Front Immunol 2021 25;12:618597. Epub 2021 Feb 25.

Laboratorio Traslacional de Inmunopatología y Oftalmología, Departamento de Patología, Facultad de Medicina, Universidad de Buenos Aires, Cuidad Autónoma de Buenos Aires, Argentina.

Air pollution is a serious environmental issue worldwide in developing countries' megacities, affecting the population's health, including the ocular surface, by predisposing or exacerbating other ocular diseases. Herpes simplex keratitis (HSK) is caused by the herpes simplex virus type 1 (HSV-1). The primary or recurring infection in the ocular site causes progressive corneal scarring that may result in visual impairment. Read More

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February 2021

IFN-λ Regulates Neutrophil Biology to Suppress Inflammation in Herpes Simplex Virus-1-Induced Corneal Immunopathology.

J Immunol 2021 Apr;206(8):1866-1877

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849;

HSV-1 infection of the cornea causes a severe immunoinflammatory and vision-impairing condition called herpetic stromal keratitis (SK). The virus replication in corneal epithelium followed by neutrophil- and CD4 T cell-mediated inflammation plays a dominant role in SK. Although previous studies demonstrate critical functions of type I IFNs (IFN-α/β) in HSV-1 infection, the role of recently discovered IFN-λ (type III IFN), specifically at the corneal mucosa, is poorly defined. Read More

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Entry receptor bias in evolutionarily distant HSV-1 clinical strains drives divergent ocular and nervous system pathologies.

Ocul Surf 2021 Mar 22. Epub 2021 Mar 22.

Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, 60612, USA; Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL, 60612, USA. Electronic address:

Purpose: Herpes simplex virus-1 (HSV-1) infection leads to varying pathologies including the development of ocular lesions, stromal keratitis and encephalitis. While the role for host immunity in disease progression is well understood, the contribution of genetic variances in generating preferential viral entry receptor usage and resulting immunopathogenesis in humans are not known.

Methods: Ocular cultures were obtained from patients presenting distinct pathologies of herpes simplex keratitis (HSK). Read More

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Innate and Adaptive Gene Single Nucleotide Polymorphisms Associated With Susceptibility of Severe Inflammatory Complications in Acanthamoeba Keratitis.

Invest Ophthalmol Vis Sci 2021 Mar;62(3):33

University College London (UCL) Institute of Ophthalmology, London, United Kingdom.

Purpose: Over a third of patients with Acanthamoeba keratitis (AK) experience severe inflammatory complications (SICs). This study aimed to determine if some contact lens (CL) wearers with AK were predisposed to SICs due to variations in key immune genes.

Methods: CL wearers with AK who attended Moorfields Eye Hospital were recruited prospectively between April 2013 and October 2014. Read More

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Detection of SARS-CoV-2 RNA in the corneal epithelium of a patient after recovery from COVID-19.

Am J Ophthalmol Case Rep 2021 Jun 16;22:101074. Epub 2021 Mar 16.

Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, USA.

Purpose: To report a case of a patient presenting with unilateral keratouveitis associated with ocular hypertension six weeks after being discharged from the hospital for COVID-19. Ocular specimens were obtained for testing.

Observations: A 69-year-old African American woman developed poor vision while hospitalized for COVID-19 in April but did not seek ophthalmic care until end of May. Read More

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Commentary: Diagnostic markers for suspected herpes simplex virus keratitis - A bridge too far.

Indian J Ophthalmol 2021 Apr;69(4):858-859

Cornea and Anterior Segment Services, MGM Eye Institute, Raipur, Chhattisgarh, India.

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Quantitative analysis of herpes simplex virus-1 transcript in suspected viral keratitis corneal buttons and its clinical significance.

Indian J Ophthalmol 2021 Apr;69(4):852-858

Ocular Microbiology, Dr Rajendra Prasad Centre for Ophthalmic Sciences, AIIMS, New Delhi, India.

Purpose: The evaluation of Herpes Simplex virus-1 (HSV-1) transcript by different investigative methods (qPCR, PCR and IHC) in corneal buttons from suspected viral keratitis patients and the comparison of results with histopathological findings and clinical diagnosis.

Methods: Sixty corneal buttons, 30 suspected viral keratitis, and 30 controls (keratoconus and bullous keratopathy) obtained after primary penetrating keratoplasty, were included in the study. All the corneal buttons were subjected to reverse transcriptase quantitative PCR (qPCR) for the detection of latency-associated transcript (LAT) gene, conventional PCR for polymerase (pol) gene, and immunohistochemistry (IHC) for HSV-1 antigen respectively. Read More

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Neurotrophic keratitis after penetrating keratoplasty for lattice dystrophy.

Am J Ophthalmol Case Rep 2021 Jun 26;22:101058. Epub 2021 Feb 26.

Storm Eye Institute, Medical University of South Carolina, Charleston, SC, USA.

Purpose: To report clinical outcomes of a patient with unilateral neurotrophic keratitis following penetrating keratoplasty for lattice dystrophy treated with topical recombinant human nerve growth factor.

Observations: A 75-year-old male with lattice dystrophy and history of herpes simplex keratitis, presented with recurrent neurotrophic ulceration in the right eye two years following penetrating keratoplasty. The patient was successfully treated with topical recombinant human nerve growth factor. Read More

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20 years since the Herpetic Eye Disease Study: Lessons, developments and applications to clinical practice.

Clin Exp Optom 2021 Apr 24;104(3):396-405. Epub 2021 Feb 24.

Faculty of Medicine and Health, University of Sydney, Sydney, Australia.

Herpes Simplex Virus (HSV) is the most common virus that causes eye disease. Although around 60% of the world's population are seropositive for HSV antigens, fortunately, it is estimated that only 1% of seropositive individuals develop eye disease. The most common ocular manifestation of HSV is keratitis, while uveitis and retinal necrosis occur in a small number of cases. Read More

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Development of Genome Editing Approaches against Herpes Simplex Virus Infections.

Viruses 2021 02 22;13(2). Epub 2021 Feb 22.

School of Public Health, University of California, Berkeley, CA 94720, USA.

Herpes simplex virus 1 (HSV-1) is a herpesvirus that may cause cold sores or keratitis in healthy or immunocompetent individuals, but can lead to severe and potentially life-threatening complications in immune-immature individuals, such as neonates or immune-compromised patients. Like all other herpesviruses, HSV-1 can engage in lytic infection as well as establish latent infection. Current anti-HSV-1 therapies effectively block viral replication and infection. Read More

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February 2021

Vaccine Development for Herpes Simplex Viruses: A Commentary of Special Issue Editors.

Vaccines (Basel) 2021 Feb 16;9(2). Epub 2021 Feb 16.

Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Via Fossato di Mortara 64/b, 44121 Ferrara, Italy.

Herpes simplex virus type 1 and 2 (HSV1 and HSV2) are global, widespread human pathogens transmitted by direct contact that cause lifelong, recurrent asymptomatic and painful symptomatic clinical illnesses (cold sores, keratitis, blepharitis, meningitis, encephalitis, genital infections), overt disease and severe sequelae in neonatal and immune-compromised patients, and increased risk of cervical cancer and other sexually transmitted infections, including HIV [... Read More

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February 2021

The Role of Tissue Resident Memory CD4 T Cells in Herpes Simplex Viral and HIV Infection.

Viruses 2021 02 25;13(3). Epub 2021 Feb 25.

Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, NSW 2145, Australia.

Tissue-resident memory T cells (TRM) were first described in 2009. While initially the major focus was on CD8 TRM, there has recently been increased interest in defining the phenotype and the role of CD4 TRM in diseases. Circulating CD4 T cells seed CD4 TRM, but there also appears to be an equilibrium between CD4 TRM and blood CD4 T cells. Read More

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February 2021

keratitis mimicking superior limbic keratoconjunctivitis and herpes simplex virus.

Am J Ophthalmol Case Rep 2021 Jun 13;22:101030. Epub 2021 Feb 13.

Department of Ophthalmology, Nassau University Medical Center, East Meadow, NY, 11554, USA.

Purpose: keratitis is a rare type of infectious keratitis and may mimic other corneal diseases and lead to delay in diagnosis. This case illustrates how often escapes accurate diagnosis due to its insidious onset, variable clinical manifestations, and unusual characteristics on cultures.

Observation: The patient presented with an epithelial defect and superior pannus and scarring, which was misdiagnosed as superior limbic keratoconjunctivitis (SLK) and herpes simplex virus (HSV) keratitis. Read More

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Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus.

Bio Protoc 2020 Sep 5;10(17):e3748. Epub 2020 Sep 5.

Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, USA.

The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA through DNA modifications such as epigenetic marks and DNA-binding proteins (, histones) as well as alteration of the chromatin state of genomic DNA within the nucleus. We recently reported a multi-step screening method for the identification of efficient sgRNAs targeting the Herpes simplex virus (HSV-1) genome and reported a differential mechanism for viral inhibition by CRISPR-Cas9 in the latent versus lytic phase. Read More

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September 2020

Efficient establishment of reactivatable latency by an acyclovir-resistant herpes simplex virus 1 thymidine kinase substitution mutant with reduced neuronal replication.

Virology 2021 Apr 3;556:140-148. Epub 2021 Feb 3.

Department of Medical Microbiology and Parasitology, and Department of Infectious Diseases of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China. Electronic address:

Herpes simplex virus 1 causes recurrent diseases by reactivating from latency, which requires the viral thymidine kinase (TK) gene. An acyclovir-resistant mutation in TK, V204G, was previously repeatedly identified in a patient with recurrent herpetic keratitis. We found that compared with its parental strain KOS, a laboratory-derived V204G mutant virus was impaired in replication in cultured neurons despite little defect in non-neuronal cells. Read More

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Quantitative real-time polymerase chain reaction analysis in herpes simplex virus keratitis with and without epithelial involvement.

Int Ophthalmol 2021 May 25;41(5):1807-1813. Epub 2021 Feb 25.

Translational Ophthalmology Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To evaluate the quantitative real-time polymerase chain reaction (qRT-PCR) analysis in herpes simplex virus (HSV) keratitis with and without epithelial involvement.

Methods: This cross-sectional study was performed on 70 patients with different HSV types and an active ocular lesion in Farabi Eye Hospital, Tehran, Iran. From these 70 patients, 171 samples were collected, including seventy tear samples and 33 scraping samples from involved eyes; and 68 samples from uninvolved fellow eyes. Read More

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Dot1l Aggravates Keratitis Induced by Herpes Simplex Virus Type 1 in Mice via p38 MAPK-Mediated Oxidative Stress.

Oxid Med Cell Longev 2021 15;2021:6612689. Epub 2021 Feb 15.

Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, 430060 Hubei, China.

Background: Disruptor of telomeric silencing 1-like (Dot1l) plays a vital role in biological processes as a well-known methyltransferase. However, its role in herpes simplex virus type 1- (HSV-1-) infected keratitis remains unclear.

Methods: and models were assessed to investigate the role of Dot1l in HSV-1 induced keratitis. Read More

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February 2021

Predisposing factors, microbiological features and outcomes of patients with clinical presumed concomitant microbial and herpes simplex keratitis.

Eye (Lond) 2021 Feb 19. Epub 2021 Feb 19.

The University of Sydney, Save Sight Institute, Discipline of Ophthalmology, Faculty of Medicine and Health, Sydney, NSW, Australia.

Background/objectives: To describe the predisposing factors, pathogens and outcomes in patients with clinical presumed concomitant microbial and herpes simplex keratitis (HSK) at Sydney Eye Hospital, Australia over a 5-year period.

Subjects/methods: A retrospective case review was conducted. Patients with clinical presumed concomitant microbial and HSK from 2012 to 2016 were identified from pathology and hospital coding databases. Read More

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February 2021

Herpes simplex virus-1 KOS-63 strain is virulent and causes titer-dependent corneal nerve damage and keratitis.

Sci Rep 2021 Feb 19;11(1):4267. Epub 2021 Feb 19.

Department of Ophthalmology, Center for Translational Ocular Immunology, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, 02111, USA.

To investigate the acute clinical, immunological, and corneal nerve changes following corneal HSV-1 KOS-63 strain inoculation. Corneas of C57BL/6 mice were inoculated with either low dose (Ld) or high dose (Hd) HSV-1 KOS-63 or culture medium. Clinical evaluation was conducted up to 7 days post inoculation (dpi). Read More

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February 2021

Herpes Simplex Virus Keratitis in a University Tertiary Referral Centre - Clinical Features and Surgical Approaches.

Klin Monbl Augenheilkd 2021 Feb 19. Epub 2021 Feb 19.

Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes und Medizinische Fakultät der Universität des Saarlandes, Homburg, Germany.

Purpose: To assess prevalence, clinical manifestations, required keratoplasties, follow-up, and outcome in patients with Herpes Simplex Virus Keratitis (HSK) attending a University Tertiary Referral Center.

Design: Retrospective (12 years), descriptive, observational study.

Methods: A total of 817 eyes with clinical diagnosis of HSK from 779 patients were classified by the type of presentation. Read More

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February 2021

Marginal epithelial herpes simplex virus keratitis-There is more than meets the "eye".

J Fr Ophtalmol 2021 Jan 13. Epub 2021 Jan 13.

Cornea, Cataract and Refractive Surgery, Dr. Shroff's Charity Eye Hospital, 5027, Kedarnath Marg, Daryaganj, New Delhi 110002, India.

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January 2021

Novel Application of Hot Melt Extrusion Technology for Preparation and Evaluation of Valacyclovir Hydrochloride Ocular Inserts.

AAPS PharmSciTech 2021 Jan 14;22(1):48. Epub 2021 Jan 14.

Department of Pharmaceutics and Drug Delivery, Pii Center for Pharmaceutical Technology, School of Pharmacy, University of Mississippi, University, Mississippi, 38677, USA.

The objective of this study was to investigate the processability of hot-melt extrusion (HME) to formulate ocular inserts of valacyclovir hydrochloride and evaluate the in vivo bioavailability of the formulation. To optimize the formulation of this drug, different physical mixtures of the polymers and plasticizer were prepared. The physical mixture was extruded through a co-rotating twin-screw extruder, and the obtained ocular inserts were cut with dimensions of 4 mm × 2 mm × 1 mm to enhance the formulation instillation in the eye. Read More

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January 2021