6,671 results match your criteria Keratitis Fungal


Systematic optimization of cationic surface engineered mucoadhesive vesicles employing Design of Experiment (DoE): A preclinical investigation.

Int J Biol Macromol 2019 Apr 17. Epub 2019 Apr 17.

Pharmaceutics Research Projects Laboratory, Department of Pharmaceutical Sciences, Dr Hari Singh Gour Central University, Sagar, M.P. 470 003, India. Electronic address:

Fungal keratitis (FK) is treated by topical natamycin (Nat) which is an effective antifungal agent. However, it has numerous therapeutic limitations i.e. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.118DOI Listing
April 2019
3 Reads

Microbial Keratitis in Stevens-Johnson Syndrome: A Prospective Study.

Cornea 2019 Apr 16. Epub 2019 Apr 16.

Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.

Purpose: To evaluate the microbiological profile and outcome in cases with infective keratitis in Stevens-Johnson syndrome (SJS).

Methods: Eighty-three eyes of 68 patients with SJS presenting with microbial keratitis were recruited and managed with standard antimicrobial therapy.

Results: Microbial keratitis developed in 34% of patients with SJS (83 eyes, 68 patients) over a period of 5 years. Read More

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http://dx.doi.org/10.1097/ICO.0000000000001960DOI Listing
April 2019
1 Read

Bandage contact lens and topical steroids are risk factors for the development of microbial keratitis after epithelium-off CXL.

BMJ Open Ophthalmol 2019 16;4(1):e000231. Epub 2019 Feb 16.

St Paul's Eye Unit, Royal Liverpool University Hospital, Liverpool, UK.

Objective: To investigate the role of bandage contact lenses (BCL) and topical steroids as risk factors for the development of microbial keratitis after epithelium-off corneal collagen cross-linking (CXL).

Methods And Analysis: Patients undergoing CXL between February 2011 and July 2017 were included. Patients were divided into two groups: those who were treated postoperatively with a BCL, topical antimicrobial and steroids (group 1) and those who received only a topical antimicrobial until healing of the epithelial defect before introduction of topical steroids (group 2). Read More

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http://dx.doi.org/10.1136/bmjophth-2018-000231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440609PMC
February 2019

Colonization of therapeutic contact lens by dematiaceous fungi.

Cont Lens Anterior Eye 2019 Apr 10. Epub 2019 Apr 10.

Department of Cornea and Refractive Services, Aravind Eye Hospital 1, Anna Nagar, Madurai, 625020, Tamil Nadu, India.

Purpose: To report 2 patients with colonization of therapeutic contact lens with dematiaceous fungi.

Methods: Case report.

Results: The first patient had a retained soft contact lens on an opaque cornea for 4 years with brownish black multiple colonies on the soft contact lens and culture grew Bipolaris spp. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13670484183104
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http://dx.doi.org/10.1016/j.clae.2019.04.008DOI Listing
April 2019
1 Read

Rational Design of Short Peptide Variants by Using Kunitzin-RE, an Amphibian-Derived Bioactivity Peptide, for Acquired Potent Broad-Spectrum Antimicrobial and Improved Therapeutic Potential of Commensalism Coinfection of Pathogens.

J Med Chem 2019 Apr 19. Epub 2019 Apr 19.

Institute of Animal Nutrition , Northeast Agricultural University , Harbin 150030 , P. R. China.

Commensalism coinfection of pathogens has seriously jeopardized human health. Currently, Kunitzin-RE, as an amphibian-derived bioactivity peptide, is regarded as a potential antimicrobial candidate. However, its antimicrobial properties were unsatisfactory. Read More

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http://pubs.acs.org/doi/10.1021/acs.jmedchem.9b00149
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http://dx.doi.org/10.1021/acs.jmedchem.9b00149DOI Listing
April 2019
3 Reads

Microbial Keratitis in Taiwan: A 20-Year update.

Am J Ophthalmol 2019 Apr 2. Epub 2019 Apr 2.

Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan. Electronic address:

Purpose: To investigate the demographics, risk factors, microbiology and resistance pattern at a tertiary hospital and to detect the shifting trend over two decades.

Design: A retrospective observational case series.

Methods: We reviewed all records of patients with microbial keratitis (MK) that were hospitalized in National Taiwan University Hospital between 2007 and 2016. Read More

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http://dx.doi.org/10.1016/j.ajo.2019.03.023DOI Listing
April 2019
1 Read

Fungal Keratitis Caused by Colletotrichum dematium: Case Study and Review.

Mycopathologia 2019 Apr 4. Epub 2019 Apr 4.

Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic.

Colletotrichum species are known as important pathogens of plants with an impact on crop production. Some of these species are also known as a cause of rare ophthalmic infections in humans. A case of keratitis caused by Colletotrichum dematium after corneal trauma in a 56-year-old woman is presented. Read More

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http://link.springer.com/10.1007/s11046-019-00335-w
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http://dx.doi.org/10.1007/s11046-019-00335-wDOI Listing
April 2019
2 Reads

keratitis: passive immunotherapy with antibodies raised against divalent flagellin.

Iran J Basic Med Sci 2019 Jan;22(1):58-64

Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.

Objectives: infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches for prevention and treatment of the infections seem to be still vital. In this report, we aimed to investigate the therapeutic effects of topical administration of the antibodies against type a and b-flagellin (FLA and FLB) in keratitis model of infection in mice.

Materials And Methods: Scratched corneas of mice were treated with approximately 10 CFUs/eye of PAK and/or PAO1 strains of . Read More

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http://dx.doi.org/10.22038/ijbms.2018.31499.7643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437458PMC
January 2019
1 Read

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains.

Ann Agric Environ Med 2019 Mar 13;26(1):198-202. Epub 2019 Mar 13.

Department of Medical Biology, Medical University, Warsaw, Poland.

Introduction: Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease - Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Read More

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http://dx.doi.org/10.26444/aaem/105394DOI Listing
March 2019
1 Read

Multi-locus DNA sequence analysis, antifungal agent susceptibility, and fungal keratitis outcome in horses from Southeastern United States.

PLoS One 2019 28;14(3):e0214214. Epub 2019 Mar 28.

Department of Clinical Sciences, NC State University, Raleigh, NC, United States of America.

Morphological characterization and multi-locus DNA sequence analysis of fungal isolates obtained from 32 clinical cases of equine fungal keratitis (FK) was performed to identify species and determine associations with antifungal susceptibility, response to therapy and clinical outcome. Two species of Aspergillus (A. flavus and A. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214214PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438541PMC

Characterization of Sex Differences in Ocular Herpes Simplex Virus 1 Infection and Herpes Stromal Keratitis Pathogenesis of Wild-Type and Herpesvirus Entry Mediator Knockout Mice.

mSphere 2019 03 27;4(2). Epub 2019 Mar 27.

Department of Microbiology and Immunology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.

Sex differences related to immune response and inflammation play a role in the susceptibility and pathogenesis of a variety of viral infections and disease (S. L. Klein, Bioessays 34:1050-1059, 2012, https://doi. Read More

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http://dx.doi.org/10.1128/mSphere.00073-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437272PMC
March 2019
2 Reads

Molecular characterization of bacterial, viral and fungal endosymbionts of Acanthamoeba isolates in keratitis patients of Iran.

Exp Parasitol 2019 Mar 24;200:48-54. Epub 2019 Mar 24.

Division of Molecular Biology & Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Free-living amoebae belong to the genus Acanthamoeba; can feed on microbial population by phagocytosis, and with the capability to act as a reservoir and a vehicle of microorganisms to susceptible host. Therefore, the role of endosymbiosis in the pathogenesis of Acanthamoeba is complex and not fully understood. The aim of the present study was to identify bacterial, fungal, and human adenovirus (HADV) endosymbionts as well as evaluating the endosymbionts role of such organisms in the pathogenesis of Acanthamoeba in keratitis patients living in Iran. Read More

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http://dx.doi.org/10.1016/j.exppara.2019.03.013DOI Listing

Mycotic Antimicrobial Localized Injection (MALIN): A Randomized Clinical Trial Evaluating Intrastromal Injection of Voriconazole.

Ophthalmology 2019 Mar 20. Epub 2019 Mar 20.

Francis I. Proctor Foundation, University of California San Francisco, USA; UCSF Department of Ophthalmology, University of California, San Francisco, USA.

Objective: To determine if there is a benefit to adjuvant intrastromal voriconazole injections (ISV) for primary treatment of filamentous fungal keratitis.

Design: Outcome-masked, randomized controlled clinical trial STUDY PARTICIPANTS: Patients presenting with moderate vision loss from a smear-positive fungal ulcer.

Intervention: Study eyes were randomized to topical natamycin plus ISV versus topical natamycin alone. Read More

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http://dx.doi.org/10.1016/j.ophtha.2019.03.020DOI Listing

Aspergillus fumigatus corneal infection is regulated by chitin synthases and by neutrophil-derived acidic mammalian chitinase.

Eur J Immunol 2019 Mar 23. Epub 2019 Mar 23.

Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio, United States.

Aspergillus fumigatus is an important cause of pulmonary and systemic infections in immune compromised individuals, and of corneal ulcers and blindness in immune competent patients. To examine the role of chitin synthases in Aspergillus corneal infection, we analyzed Aspergillus mutants of chitin synthase family 1 and family 2, and found that the quadruple mutants from both families were more readily killed by neutrophils in vitro, and that both exhibited an impaired ability to grow in the cornea. Further, inhibition of chitin synthases using Nikkomycin Z enhanced neutrophil killing in vitro and in vivo in a murine model of A. Read More

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http://dx.doi.org/10.1002/eji.201847851DOI Listing

Visual impairment in fungal versus bacterial corneal ulcers four years after successful antimicrobial treatment.

Am J Ophthalmol 2019 Mar 19. Epub 2019 Mar 19.

Department of Ophthalmology; Francis I. Proctor Foundation. Electronic address:

Purpose: To compare longitudinal visual acuity outcomes of fungal corneal ulcers with those of bacterial ulcers.

Design: Prospective cohort study SETTING: Tertiary eye hospital in South India STUDY POPULATION: 100 individuals who had been diagnosed four years prior with either fungal or bacterial keratitis and had been enrolled in one of two concurrent randomized trials, out of 152 consecutive participants eligible for the study.

Exposure: Causative organism of infectious keratitis (bacteria versus fungus) MAIN OUTCOME MEASURES: Presenting visual acuity, best spectacle corrected visual acuity (BSCVA), and hard contact lens-corrected visual acuity (CLVA). Read More

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http://dx.doi.org/10.1016/j.ajo.2019.03.010DOI Listing

Predicting factors and prediction model for discriminating between fungal infection and bacterial infection in severe microbial keratitis.

PLoS One 2019 20;14(3):e0214076. Epub 2019 Mar 20.

Department of Clinical Epidemiology and Biostatistics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

A retrospective medical record review including 344 patients who were admitted with severe microbial keratitis at Ramathibodi Hospital, Bangkok, Thailand, from January 2010 to December 2016 was conducted. Causative organisms were identified in 136 patients based on positive culture results, pathological reports and confocal microscopy findings. Eighty-six eyes (63. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214076PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426210PMC

Severe mycotic keratoconjunctivitis caused by sp. in an immunocompetent child successfully treated with intravenous voriconazole and keratoplasty: case report and short review of the literature.

Ther Adv Infect Dis 2019 Jan-Dec;6:2049936118811213. Epub 2018 Nov 12.

University of Texas Rio Grande Valley, Rio Grande Valley, TX, USA.

Background: Pediatric mycotic infections in the eye are uncommon. However, ophthalmic infections by several fungal species have been described in immunocompetent subjects. Mycotic keratitis with or without conjunctivitis (MK) may account for more than 50% of all cases, particularly in tropical and sub-tropical areas. Read More

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http://dx.doi.org/10.1177/2049936118811213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416680PMC
November 2018

Post-traumatic endophthalmitis caused by .

JMM Case Rep 2019 Feb 20;6(2):e005175. Epub 2019 Feb 20.

Departament of Microbiology, University Hospital Marqués de Valdecilla - IDIVAL, Santander, Spain.

Introduction: complex has been associated with infections in both immunocompetent and immunocompromised patients. Infection can be localized or disseminated, affecting skin and soft tissues, the respiratory system, bones and joints, the circulatory system and especially the central nervous system. Ocular infections such as keratitis, scleritis, conjunctivitis, dacryocystitis, orbital cellulitis and endophthalmitis due to spp. Read More

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http://dx.doi.org/10.1099/jmmcr.0.005175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421342PMC
February 2019
3 Reads

Equine ulcerative keratitis with furrow: A review of the outcomes of 72 cases from 1987 to 2015.

Equine Vet J 2019 Mar 18. Epub 2019 Mar 18.

Comparative Ophthalmology, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.

Background: Ulcerative keratitis with peripheral furrow formation is a poorly-described condition which has been associated with a grave prognosis due to rapid necrosis of the cornea.

Objective: To describe the infectious aetiologies associated with furrow-forming ulcerative keratitis, its overall clinical course and the efficacy of medical and surgical intervention in horses.

Study Design: Retrospective clinical case series. Read More

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http://dx.doi.org/10.1111/evj.13101DOI Listing

Fungal keratitis caused by : A first case report.

Indian J Med Microbiol 2018 Oct-Dec;36(4):594-596

Department of Ocular Microbiology, Aravind Eye Hospital and Post Graduate Institute of Ophthalmology, Madurai, Tamil Nadu, India.

We report a case of keratitis caused by a rare fungus Podospora austroamericana. Clinical and microbiological evaluation of the corneal ulcer was done and the treatment outcome was studied. The fungus was grown from the corneal scraping, and it was identified as P. Read More

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http://dx.doi.org/10.4103/ijmm.IJMM_19_1DOI Listing

Exserohilum keratitis: Clinical profile of nine patients and comparison of morphology versus ITS-Based DNA sequencing for species identification of the fungal isolates.

Indian J Med Microbiol 2018 Oct-Dec;36(4):564-568

Ophthalmic Pathology Laboratory, Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, Telangana, India.

Purpose: The objective of this study was to describe the microbiological and clinical features of nine cases of Exserohilum keratitis.

Patients And Methods: Fungal isolates from corneal scrapings were identified based on macroscopic and microscopic characteristics of the colonies and DNA sequencing of ITS1-5.8S-ITS2 region in the rRNA gene. Read More

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http://dx.doi.org/10.4103/ijmm.IJMM_19_51DOI Listing
March 2019
1.037 Impact Factor

Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection.

Exp Eye Res 2019 Mar 8;182:19-29. Epub 2019 Mar 8.

Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, Shandong, PR China. Electronic address:

Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize pathogen-associated molecular patterns to initiate downstream signal cascades to active immune responses. Our previous studies reported that, in vitro human cornea epithelium cells represented a novel target of TSLP and that TSLP/TSLPR/STAT5 signaling played an important role in the response to Aspergillus fumigatus challenge. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.020DOI Listing
March 2019
2 Reads

Proteome analysis of biofilm produced by a Fusarium falciforme keratitis infectious agent.

Microb Pathog 2019 May 6;130:232-241. Epub 2019 Mar 6.

Laboratorio de Microbiología y Proteómica, Instituto de Oftalmología "Fundación de Asistencia Privada Conde de Valenciana", Mexico City, Mexico. Electronic address:

Biofilms are structures that confer adaptive ability to and facilitate the virulence of fungal pathogens. Certain multi-functional proteins have been shown to be involved in fungal pathogenesis and these proteins may also be implicated in biofilm formation. The aim of this study was to identify a fungal agent isolated from the human cornea, to analyze the ability of this organism to form biofilms in vitro and to investigate protein expression in this condition. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.03.001DOI Listing
May 2019
2 Reads
2.000 Impact Factor

Differential Expression of Antimicrobial Peptides in Streptococcus pneumoniae Keratitis and STAT3-Dependent Expression of LL-37 by Streptococcus pneumoniae in Human Corneal Epithelial Cells.

Pathogens 2019 Mar 6;8(1). Epub 2019 Mar 6.

Prof. Brien Holden Eye Research Center, LV Prasad Eye Institute, Hyderabad 500034, India.

is the leading cause of bacterial keratitis in the developing world with a growing trend of acquiring resistance against various antibiotics. In the current study, we determined the expression of different antimicrobial peptides (AMPs) in response to in patients, as well as in primary and immortalized human corneal epithelial cells. We further focused on LL-37 and determined its expression in human cornea infected with and studied the killing ability of LL-37 against The expression of AMPs was determined by quantitative PCR and the phosphorylation of signaling proteins was evaluated by immunoblot analysis. Read More

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http://dx.doi.org/10.3390/pathogens8010031DOI Listing
March 2019
1 Read

Microbial Keratitis in Thyroid Eye Disease: Clinical Features, Microbiological Profile, and Treatment Outcome.

Ophthalmic Plast Reconstr Surg 2019 Mar 5. Epub 2019 Mar 5.

Ophthalmic Plastic Surgery Service, L.V. Prasad Eye Institute, Hyderabad, India; Jhaveri Microbiology laboratory, L.V. Prasad Eye Institute, Hyderabad, India; and Tej Kohli Cornea Insititute, L.V. Prasad Eye Institute, Hyderabad, India.

Purpose: To report the incidence, clinical features, microbiologic profile, and risk indicators in the development of microbial keratitis in Thyroid Eye Disease (TED).

Methods: All patients who were diagnosed to have TED and developed microbial keratitis between the years 2009 to 2017 at the Ophthalmic Plastic Surgery service, LV Prasad Eye Institute were included in this retrospective interventional study. The clinical features, microbiological profile, and treatment outcome of the infection were studied. Read More

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http://dx.doi.org/10.1097/IOP.0000000000001361DOI Listing
March 2019
2 Reads

[Fungal keratitis caused by Colletotrichum gloeosporioides: A case report].

Rev Esp Quimioter 2019 Apr 6;32(2):198-199. Epub 2019 Mar 6.

Ismail Zakariya-Yousef Breval, Hospital Juan Ramón Jiménez, Ronda exterior norte s/n, 21005, Huelva. Spain.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441994PMC
April 2019
1 Read

Endophthalmitis associated with fungal keratitis and penetrating injuries in North China.

Eur J Ophthalmol 2019 Mar 4:1120672119833896. Epub 2019 Mar 4.

3 Clinical Lab of Qilu Hospital (Qingdao), Shandong University, Qingdao, P.R.China.

Purpose:: To report the laboratory findings, management strategies, and visual outcomes of culture-proven exogenous fungal endophthalmitis in North China.

Methods:: The microbiological and treatment records of patients with culture-positive exogenous fungal endophthalmitis who visited the Affiliated Hospital of Qingdao University from January 2012 to December 2016 were reviewed.

Results:: A total of 39 eyes (39 patients) were identified over a 5-year period. Read More

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http://dx.doi.org/10.1177/1120672119833896DOI Listing
March 2019
3 Reads

Successful treatment of Fusarium solani anterior chamber involvement secondary to contact lens associated corneal ulcer with intracameral Amphotericin B.

GMS Ophthalmol Cases 2019 12;9:Doc03. Epub 2019 Feb 12.

Department of Ophthalmology, University Hospital Regensburg, Germany.

spp. are leading fungal pathogenes in contact lens associated keratitis and may evoke endophthalmitis. Since spp. Read More

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http://dx.doi.org/10.3205/oc000092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381420PMC
February 2019
1 Read

Staphylococcus aureus alpha-hemolysin impairs corneal epithelial wound healing and promotes intracellular bacterial invasion.

Exp Eye Res 2019 Apr 27;181:263-270. Epub 2019 Feb 27.

Stem Cell Therapy and Corneal Tissue Engineering Laboratory, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1905 W. Taylor St., L-213, Chicago, IL, 60612, United States. Electronic address:

Colonization by Staphylococcus aureus (S. aureus) has been implicated in many infectious and wound healing disorders. This study was performed to characterize the pathogenic role of S. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447303PMC
April 2019
5 Reads
2.709 Impact Factor

Corneal lymphangiogenesis ameliorates corneal inflammation and edema in late stage of bacterial keratitis.

Sci Rep 2019 Feb 27;9(1):2984. Epub 2019 Feb 27.

Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan.

Lymphatic vessels play a crucial role in systemic immune response and regulation of tissue fluid homeostasis. Corneal lymphangiogenesis in bacterial keratitis has not been studied. In this study, we investigated the mechanism and the role of corneal lymphangiogenesis in a murine bacterial keratitis model using Pseudomonas aeruginosa. Read More

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http://dx.doi.org/10.1038/s41598-019-39876-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393676PMC
February 2019
2 Reads

[The German keratomycosis registry : Initial results of a multicenter survey].

Ophthalmologe 2019 Feb 27. Epub 2019 Feb 27.

Klinik für Augenheilkunde, Universitätsaugenklinik Düsseldorf, Moorenstr. 5, 40225, Düsseldorf, Deutschland.

Background And Purpose: Mycotic keratitis is a serious but relatively rare disease. No targeted data collection in Germany existed until the foundation of the German Pilz-Keratitis Register in 2015.

Patients And Methods: The inclusion of retrospective and prospective patients was carried out. Read More

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http://link.springer.com/10.1007/s00347-019-0871-9
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http://dx.doi.org/10.1007/s00347-019-0871-9DOI Listing
February 2019
6 Reads

Conjunctival microbiome changes associated with fungal keratitis: metagenomic analysis.

Int J Ophthalmol 2019 18;12(2):194-200. Epub 2019 Feb 18.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao 266071, Shandong Province, China.

Aim: To investigate the ocular surface microbiome profile of patients with fungal keratitis (FK) through bacterial 16S rDNA sequencing.

Methods: The swab samples were collected from 8 patients with FK (Group 1 from the corneal ulcer, Group 2 from the conjunctival sac of the infected eyes, and Group 3 from the conjunctival sac of the fellow eyes) and 10 healthy eyes (Group 4 from the conjunctival sac). Bacterial 16S rDNA V4-V5 region sequencing was performed to characterize the bacterial communities on the ocular surfaces of the patients with FK. Read More

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http://dx.doi.org/10.18240/ijo.2019.02.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376244PMC
February 2019

Mechanisms of Fluoroquinolone and Aminoglycoside Resistance in Keratitis-Associated Pseudomonas aeruginosa.

Microb Drug Resist 2019 Feb 26. Epub 2019 Feb 26.

1 Department of Ocular Microbiology and Aravind Medical Research Foundation, Madurai, India.

Global emergence of multidrug resistant (MDR) strains limits therapeutic efficacy in Pseudomonas aeruginosa corneal ulcers. Identifying the primary causal factors of resistance shall improve clinical management. In this study, we sought to identify the underlying mechanisms of fluoroquinolone and aminoglycoside resistance in MDR, non-MDR, and drug susceptible P. Read More

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http://dx.doi.org/10.1089/mdr.2018.0218DOI Listing
February 2019
1 Read

Clinical outcomes in ocular pythiosis patients treated with a combination therapy protocol in Thailand: A prospective study.

Med Mycol 2019 Feb 26. Epub 2019 Feb 26.

Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Ocular pythiosis is the second most common form of human pythiosis, and the rates of evisceration/enucleation in Thailand are 55-79%. This prospective study was conducted to evaluate treatment outcomes of the combination therapy protocol and the potential use of serum (1→3)-β-glucan (BG) and Pythium insidiosum-specific antibody (Pi-Ab) as an aid to diagnosis and monitoring of ocular pythiosis. Thirty patients were enrolled in the study and 14 (non-globe salvage) required evisceration/enucleation. Read More

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http://dx.doi.org/10.1093/mmy/myz013DOI Listing
February 2019
2 Reads

Trends in contact lens microbial keratitis 1999-2015; a retrospective clinical review.

Clin Exp Ophthalmol 2019 Feb 22. Epub 2019 Feb 22.

School of Optometry and Vision Science, University of New South Wales, Australia.

Importance: Contact lens microbial keratitis (CLMK) is the most common cause of microbial keratitis in our community.

Background: Define the trend in rate of CLMK and define patient demographics/culture results that may have a predictive value in patients with CLMK DESIGN: Retrospective review of clinical records of patients with MK PARTICIPANTS: All patients with positive corneal scraping between 1999-2015 at the Princess Alexandra Hospital, Brisbane, Queensland identified through local microbiology database METHODS: Trend in CLMK tested with chi-squared test of peak 3 years vs other years and Poisson regression of interrupted time series. Patient characteristics predictive of CLMK were defined by creating a polynomial regression model by stepwise variable selection. Read More

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http://dx.doi.org/10.1111/ceo.13484DOI Listing
February 2019
5 Reads

Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of and Isolates from Corneal Scrapings.

Biomed Res Int 2019 20;2019:6395840. Epub 2019 Jan 20.

Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Coimbatore, India.

Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly, and are reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis patients, of which 411 (82. Read More

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http://dx.doi.org/10.1155/2019/6395840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360544PMC
January 2019
1 Read

Povidone-iodine concentration and in vitro killing time of bacterial corneal ulcer isolates.

Digit J Ophthalmol 2018 19;24(4):24-26. Epub 2018 Dec 19.

Albany Medical College, Albany, New York.

Background: The concentration and dosing of povidone-iodine (PI) solution used in surgical site prophylaxis are variable. Prior in vitro work has demonstrated that dilute PI solutions (<1%) had greater bactericidal activity than stock solutions (10%). Studies using pathologic clinical isolates from the eye have yielded mixed results. Read More

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http://dx.doi.org/10.5693/djo.01.2018.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380258PMC
March 2019
1 Read

Pharmacologic therapy of mycotic keratitis.

Surv Ophthalmol 2019 Feb 22. Epub 2019 Feb 22.

Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Mycotic keratitis continues to be an important cause of corneal blindness, especially in tropical and subtropical countries. The prognosis is poor compared with many other forms of keratitis because of the lack of effective antifungal drugs. The currently available antifungal drugs suffer from multiple drawbacks such as poor ocular penetration, unpredictable bioavailability, and adverse effects associated with systemic medications. Read More

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http://dx.doi.org/10.1016/j.survophthal.2019.02.007DOI Listing
February 2019
1 Read

Pannexin 1 Channels Contribute to IL-1β Expression via NLRP3/Caspase-1 Inflammasome in Aspergillus Fumigatus Keratitis.

Curr Eye Res 2019 Feb 22:1-10. Epub 2019 Feb 22.

a Department of Ophthalmology , The Affiliated Hospital of Qingdao University , Qingdao , Shandong Province , China.

Purpose: Pannexin 1 channels are deemed to play important roles in inflammation. However, there is limited information regarding their roles in fungal infection diseases, especially fungal keratitis. This study aimed to investigate the role of pannexin 1 channels in Aspergillus fumigatus (A. Read More

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http://dx.doi.org/10.1080/02713683.2019.1584321DOI Listing
February 2019
3 Reads

Donor-Related Nontuberculous Mycobacterial Interface Infection After Descemet Membrane Endothelial Keratoplasty.

Cornea 2019 May;38(5):632-634

Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium.

Purpose: To describe the first reported case of Mycobacterium chelonae-related interface keratitis after Descemet membrane endothelial keratoplasty (DMEK), successfully treated with DMEK exchange.

Methods: A case of donor-related DMEK interface keratitis, treated with medical therapy and DMEK exchange, was studied retrospectively.

Results: A patient with Fuchs endothelial dystrophy developed infectious interface keratitis after DMEK. Read More

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http://dx.doi.org/10.1097/ICO.0000000000001895DOI Listing
May 2019
2 Reads

Natamycin niosomes as a promising ocular nanosized delivery system with ketorolac tromethamine for dual effects for treatment of candida rabbit keratitis; in vitro/in vivo and histopathological studies.

Drug Dev Ind Pharm 2019 Feb 12:1-15. Epub 2019 Feb 12.

c Department of surgical pathology , Faculty of Medicine, Cairo University , Cairo , Egypt.

Objectives: This study was aimed to develop dual-purpose natamycin (NAT)-loaded niosomes in ketorolac tromethamine (KT) gels topical ocular drug delivery system to improve the clinical efficacy of natamycin through enhancing its penetration through corneal tissue and reducing inflammation associated with Fungal keratitis (FK).

Significance: Nanosized carrier systems, as niosomes would provide great potential for improving NAT ocular bioavailability.NAT niosomal dispersion formulae were prepared and then incorporated in 0. Read More

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http://dx.doi.org/10.1080/03639045.2019.1579827DOI Listing
February 2019
8 Reads

A case of recurrent keratitis caused by Paecilomyces lilacinus and treated by voriconazole.

Arq Bras Oftalmol 2019 Mar-Apr;82(2):152-154

Department of Ophthalmology and Visual Sciences, Universidade Federal de São Paulo, São Paulo, SP, Brazil.

We describe here a case of a 21-year-old woman who presented with low visual acuity, pain, and hyperemia in the left eye for 45 days. Her eye had extensive corneal infiltrate, with melting and a central perforation that was glued with cyanoacrylate, but with Seidel (+). She underwent tectonic corneal transplantation, and anterior chamber lavage with subconjunctival infiltration with voriconazole, as well as intracameral injections of amphotericin B. Read More

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http://www.gnresearch.org/doi/10.5935/0004-2749.20190031
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http://dx.doi.org/10.5935/0004-2749.20190031DOI Listing
July 2018
10 Reads

The Acanthamoeba- Fungal keratitis study.

Am J Ophthalmol 2019 Feb 2. Epub 2019 Feb 2.

Aravind Eye Hospital and Post-Graduate Institute of Ophthalmology, Coimbatore, India 641014.

Purpose: To ascertain the incidence of Acanthamoeba Keratitis, and the co-existence of Acanthamoeba & fungi in microbial keratitis Design; A prospective cross-sectional study METHODS: Patients presenting with stromal keratitis were additionally tested for Acanthamoeba irrespective of the clinical diagnosis. Culture positivity was the gold standard RESULTS: Of the 401 cases included in the study, 40 were positive for Acanthamoeba (10 %), 16 of these 40 were positive for both Acanthamoeba and fungi (4.5% of the Study Group was Acanthamoeba and fungal Keratitis positive); 5/40 for Acanthamoeba and bacteria; 2/40 had triple infection with Acanthamoeba, fungi and bacteria. Read More

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http://dx.doi.org/10.1016/j.ajo.2019.01.024DOI Listing
February 2019
3 Reads

Clinical and microbiological characteristics of Moraxella keratitis.

Br J Ophthalmol 2019 Feb 1. Epub 2019 Feb 1.

Ophthalmology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland

Background/aims: To describe the risk factors, clinical features, bacterial subspecies characteristics and treatment outcomes of Moraxella keratitis in a single centre.

Methods: A retrospective review of all patients diagnosed with Moraxella keratitis between November 2012 and December 2017 at the Royal Victoria Eye and Ear Hospital, Dublin, Ireland was performed. Matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry was used to identify subspecies. Read More

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http://dx.doi.org/10.1136/bjophthalmol-2018-313557DOI Listing
February 2019
2 Reads

A ten year study of prevalence, antimicrobial susceptibility pattern, and genotypic characterization of Methicillin resistant Staphylococcus aureus causing ocular infections in a tertiary eye care hospital in South India.

Infect Genet Evol 2019 Apr 30;69:203-210. Epub 2019 Jan 30.

Department of Ocular Microbiology, Aravind Eye Hospital, Madurai, India. Electronic address:

Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of vision threatening ocular infections. This study aimed to determine the prevalence and antimicrobial susceptibility pattern of MRSA and their genotypic characterization in ocular infections. The study period was from January 2007 to December 2017 in Aravind Eye Hospital, Madurai. Read More

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http://dx.doi.org/10.1016/j.meegid.2019.01.031DOI Listing
April 2019
2 Reads

Designing and enhancing the antifungal activity of corneal specific cell penetrating peptide using gelatin hydrogel delivery system.

Int J Nanomedicine 2019 15;14:605-622. Epub 2019 Jan 15.

Department of Nanobiotechnology, Vision Research Foundation, Sankara Nethralaya campus, Chennai, Tamil Nadu, India,

Background: Fungal keratitis is a major cause of corneal blindness accounting for more than one-third of microbiologically proven cases. The management of fungal keratitis is through topical or systemic antifungal medications alone or in combination with surgical treatment. Topical medications such as natamycin and voriconazole pose major challenges due to poor penetration across the corneal epithelium. Read More

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https://www.dovepress.com/designing-and-enhancing-the-antifu
Publisher Site
http://dx.doi.org/10.2147/IJN.S184911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339655PMC
February 2019
9 Reads

Maresin1 regulates neutrophil recruitment and IL-10 expression in Aspergillus fumigatus keratitis.

Int Immunopharmacol 2019 Apr 25;69:103-108. Epub 2019 Jan 25.

Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. Electronic address:

Purpose: Maresin1, a lipid mediator derived from polyunsaturated fatty acids, has been shown to suppress the inflammatory response in various inflammatory diseases. However, its effects in fungal keratitis are still uncertain. In this study, we investigated the role of maresin1 (MaR1) in Aspergillus fumigatus keratitis of the eye in a mouse model. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.01.032DOI Listing
April 2019
2 Reads

IL-17 produced by Th17 cells alleviates the severity of fungal keratitis by suppressing CX43 expression in corneal peripheral vascular endothelial cells.

Cell Cycle 2019 Feb 20;18(3):274-287. Epub 2019 Jan 20.

a Department of Ophthalmology , The First Affiliated Hospital of Dalian Medical University , Dalian , P. R. China.

Fungal keratitis is a relatively common ocular disease requiring positive medical management combined with surgical intervention. Interleukin-17 (IL-17) was reported to promote the activation and mobilization of neutrophile granulocyte to foci of inflammation. This study investigated the effect of IL-17 production from Th17 cells on the progression of fungal keratitis. Read More

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https://www.tandfonline.com/doi/full/10.1080/15384101.2018.1
Publisher Site
http://dx.doi.org/10.1080/15384101.2018.1556059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380429PMC
February 2019
11 Reads

Corneal Cross-Linking Window Absorption (CXL-WA) as an Adjuvant Therapy in the Management of Keratitis.

Case Rep Ophthalmol Med 2018 10;2018:4856019. Epub 2018 Dec 10.

Humanitas Clinical and Research Center, Via Manzoni 56, Rozzano, Milano, Italy.

Purpose: To evaluate the effectiveness of corneal cross-linking window absorption (CXL-WA) as an adjuvant therapy for keratitis.

Methods: A 90-year-old male came to our clinic complaining of hyperemic conjunctivitis and progressive visual loss in the right eye. Slit-lamp examination showed keratic precipitates, severe corneal opacity, and stromal edema. Read More

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https://www.hindawi.com/journals/criopm/2018/4856019/
Publisher Site
http://dx.doi.org/10.1155/2018/4856019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311300PMC
December 2018
9 Reads