1,733 results match your criteria Journal of tissue engineering and regenerative medicine[Journal]


Strategies for controlling egress of therapeutic cells from hydrogel microcapsules.

J Tissue Eng Regen Med 2019 Feb 16. Epub 2019 Feb 16.

Department of Physics, University of Ottawa, Ottawa, Canada.

Endothelial progenitor cells (EPCs) and human mesenchymal stem cells (hMSCs) have shown great regenerative potential to repair damaged tissue; however, their injection in vivo results in low retention and poor cell survival. Early clinical research has focused on cell-encapsulation, to improve viability and integration of delivered cells. However, this strategy has been limited by the inability to reproduce large volumes of standardized microcapsules and the lack of information on cell-specific egress and timed release from hydrogel microcapsules. Read More

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http://dx.doi.org/10.1002/term.2818DOI Listing
February 2019

Co-culture with monocytes/macrophages modulates osteogenic differentiation of adipose-derived mesenchymal stromal cells on PLGA/PCL scaffolds.

J Tissue Eng Regen Med 2019 Feb 16. Epub 2019 Feb 16.

Department of Biomaterials, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands.

The effects of immune cells, in particular macrophages, on the behavior of mesenchymal stem cells (MSCs) have recently gained much attention for MSCs-based tissue-engineered constructs. This study aimed to evaluate the effect of monocytes/macrophages on the osteogenic differentiation of adipose-derived mesenchymal stromal cells (ADMSCs) in three-dimensional (3D) co-cultures. For this, we co-cultured THP-1 monocytes, M1 macrophages or M2 macrophages with ADMSCs on 3D poly (lactic-co-glycolic) acid (PLGA)/polycaprolactone (PCL) scaffolds using osteogenic medium for up to 42 days. Read More

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http://doi.wiley.com/10.1002/term.2826
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http://dx.doi.org/10.1002/term.2826DOI Listing
February 2019
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Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.

Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents. A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue engineered products. The objectives of this study were to 1) determine whether matrix stabilization with exogenous lysyl oxidase like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, 2) evaluate whether label-free, non-destructive, Fluorescence Lifetime Imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, 3) form quantitative relationships between destructive and nondestructive measurements to determine whether the strength of these correlations are sufficient to replace destructive testing methods, and 4) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking. Read More

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http://dx.doi.org/10.1002/term.2824DOI Listing
February 2019

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, People's Republic of China.

Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin-1 (Ang-1) dual gene co-expression vector that encoded green fluorescent protein (GFP) was constructed from an Arg-Gly-Asp-modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze-drying method. Read More

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http://dx.doi.org/10.1002/term.2819DOI Listing
February 2019

Low-intensity pulsed ultrasound combination with iPSCs-NCSCs and GDF5 promotes sciatic nerve regeneration and functional recovery.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, P. R. China.

The treatment of lengthy peripheral nerve defect is challenging in the field of nerve regeneration. Our previous studies have shown that low-intensity pulsed ultrasound (LIPUS) could promote the proliferation, cell viability, and neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) and improve the regeneration of damaged peripheral nerve. In this study, the mechanical signal transduction pathway of LIPUS promoting iPSCs-NCSCs proliferation and differentiation was further explored, and the effects of LIPUS combined with iPSCs-NCSCs, perfluorotributylamine (PFTBA), and growth differentiation factor 5 (GDF5) on the repair of peripheral nerve injury were evaluated. Read More

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http://dx.doi.org/10.1002/term.2823DOI Listing
February 2019

Impact of modified gelatin on valvular microtissues.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Department of Human Structure and Repair, Tissue Engineering Group, Ghent University, Ghent, Belgium.

A significant challenge in the field of tissue engineering is the biofabrication of three-dimensional (3D) functional tissues with direct applications in organ-on-a-chip systems and future organ engineering. Multi-cellular valvular microtissues can be used as building blocks for the formation of larger-scale valvular macrotissues. Yet, for the controlled biofabrication of 3D macrotissues with predefined complex shapes, directed assembly of microtissues through bioprinting is needed. Read More

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http://dx.doi.org/10.1002/term.2825DOI Listing
February 2019

Strategic design of peptide-decorated aligned nanofibers impregnated with triiodothyronine for neural regeneration.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Biological Materials Laboratory, CSIR - Central Leather Research Institute, Chennai, India.

Nerve injuries are often debilitating as its regeneration occurs in a slow and laborious manner. Remediation of nerve injury is a colossal task as functional restoration in larger gaps seldom occurs due to the complex nerve regeneration mechanism. A nanofiber-based graft material has been fabricated to provide topographical and biochemical cues to encourage neural differentiation. Read More

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http://dx.doi.org/10.1002/term.2822DOI Listing
February 2019

Serum albumin coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats.

J Tissue Eng Regen Med 2019 Feb 12. Epub 2019 Feb 12.

Institute of Clinical Experimental Research, Semmelweis University, Budapest, Hungary.

Serum albumin coated bone allografts (BoneAlbumin) have successfully supported bone regeneration in various experimental models by activating endogenous progenitors. However, the effect of tissue aging, linked to declining stem cell function, has yet to be explicitly examined within the context of BoneAlbuminꞋs regenerative capacity. Stem cell function was tested with an in vitro attachment assay, which showed that albumin coating increases stem cell attachment on demineralized bone surfaces in an aging cell population. Read More

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http://dx.doi.org/10.1002/term.2803DOI Listing
February 2019

Differentiation of the human liver progenitor cell line (HepaRG) on a microfluidic-based biochip.

J Tissue Eng Regen Med 2019 Feb 12. Epub 2019 Feb 12.

Department of system engineering, Saarland University, Saarbrücken, Germany.

HepaRG is a bipotent stem cell line that can be differentiated toward hepatocyte-like and biliary-like cells. The entire cultivation process requires one month and relies on the addition of 2 % dimethyl sulfoxide (DMSO) to the culture. Our motivation in this research is to differentiate HepaRG cells (progenitor cells, undifferentiated cells) toward hepatocyte-like cells by minimizing the cultivation time and without using DMSO treatment by instead using a microfluidic device combined with the following strategies: i) comparison of extracellular matrices (matrigel, collagen I), ii) types of flow (one or both sides), and iii) effects of DMSO. Read More

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http://dx.doi.org/10.1002/term.2802DOI Listing
February 2019

Biohybrids for spinal cord injury repair.

J Tissue Eng Regen Med 2019 Feb 6. Epub 2019 Feb 6.

Neuronal and Tissue Regeneration Laboratory, Prince Felipe Research Center, Valencia, Spain.

Spinal cord injuries (SCI) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and chronic SCI in experimental models, via neuronal differentiation and trophic glial cell support. Here we propose an improved procedure based on transplantation of epSPC in a tubular conduit of hyaluronic acid (HA) containing poly (lactic acid) (PLLA) fibers creating a biohybrid scaffold. Read More

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http://dx.doi.org/10.1002/term.2816DOI Listing
February 2019
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Short term effectiveness of platelet-rich plasma in carpal tunnel syndrome: A controlled study.

J Tissue Eng Regen Med 2019 Feb 1. Epub 2019 Feb 1.

Department of Physical and Rehabilitation Medicine, Hacettepe University Medical School, Ankara, Turkey.

PRP treatment has a potential to become a part of non-surgical approach in CTS as a regenerative method. Platelet-rich plasma (PRP) therapies aim to enhance the self-healing ability of human body, by exposing the injured tissue to a high concentration of autologous growth factors. Nerve tissues also seem to benefit from the regenerative effects of PRP concentrates. Read More

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http://dx.doi.org/10.1002/term.2815DOI Listing
February 2019
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Injectable freeze-dried chitosan/PRP implants improve marrow stimulated cartilage repair in a chronic defect rabbit model.

J Tissue Eng Regen Med 2019 Feb 1. Epub 2019 Feb 1.

Biomedical Engineering Institute, Ecole Polytechnique, Montreal, QC, Canada.

Bone Marrow Stimulation (BMS) improves knee joint function but elicits incomplete repair. Liquid chitosan (CS)-glycerol phosphate/blood clots have been shown to improve BMS-based cartilage repair. Platelet-rich-plasma (PRP) - a rich source of growth factors and cytokines - improves recruitment and chondrogenic potential of subchondral mesenchymal stem cells. Read More

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http://dx.doi.org/10.1002/term.2814DOI Listing
February 2019

A magnetic three-dimensional levitated primary cell culture system for the development of secretory salivary gland-like organoids.

J Tissue Eng Regen Med 2019 Jan 21. Epub 2019 Jan 21.

Nano3D Biosciences Inc, University of Texas Health Sciences Center at Houston, Houston, Texas, USA, Houston, Texas, USA.

Salivary gland (SG) hypofunction and oral dryness can be induced by radiotherapy for head and neck cancers or auto-immune disorders. These are common clinical conditions that involve loss of saliva-secreting epithelial cells. Several oral complications arise with SG hypofunction that interfere with routine daily activities like chewing, swallowing and speaking. Read More

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http://dx.doi.org/10.1002/term.2809DOI Listing
January 2019
1 Read

In vitro cellular testing of Strontium/Calcium substituted phosphate glass discs and microspheres shows potential for bone regeneration.

J Tissue Eng Regen Med 2019 Jan 21. Epub 2019 Jan 21.

Wolfson STEM Centre, School of Medicine, University of Nottingham, Nottingham, UK.

Phosphate glasses (PBG) are ideal materials for regenerative medicine strategies since their composition, degradation rates and ion release profiles can easily be controlled. Strontium has previously been found to simultaneously affect bone resorption and deposition. Therefore, by combining the inherent properties of resorbable PBG and therapeutic activity of strontium, these glasses could be used as a delivery device of therapeutic factors for the treatment of orthopaedic diseases such as osteoporosis. Read More

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http://doi.wiley.com/10.1002/term.2796
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http://dx.doi.org/10.1002/term.2796DOI Listing
January 2019
4 Reads

Visualizing Cell-Laden Fibrin-Based Hydrogels using Cryogenic Scanning Electron Microscopy and Confocal Microscopy.

J Tissue Eng Regen Med 2019 Jan 18. Epub 2019 Jan 18.

Departments of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.

The present investigation explores the microscopic aspects of cell-laden hydrogels at high resolutions, using three-dimensional cell cultures in semi-synthetic constructs that are of very-high water content (>98% water). The study aims to provide an imaging strategy for these constructs, while minimizing artifacts. Constructs of PEG-fibrinogen (PEG-Fb) and fibrin hydrogels containing embedded mesenchymal cells (human dermal fibroblasts) were first imaged by confocal microscopy. Read More

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http://dx.doi.org/10.1002/term.2813DOI Listing
January 2019

Reconstructive urology and tissue engineering: converging developmental paths.

J Tissue Eng Regen Med 2019 Jan 18. Epub 2019 Jan 18.

Chair of Urology, Department of Regenerative Medicine, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.

Reconstructive urology is a complex and demanding branch of modern urology. Complicated cases, necessity of microsurgical approach and constant exposure to urine make urinary reconstruction especially difficult. With impaired healing, excessive scarring and recurring fibrosis functional results are still not satisfying. Read More

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http://dx.doi.org/10.1002/term.2812DOI Listing
January 2019

Promising effects of exosomes isolated from menstrual blood-derived mesenchymal stem cell on wound healing process in diabetic mouse model.

J Tissue Eng Regen Med 2019 Jan 18. Epub 2019 Jan 18.

Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Wound healing is a complicated process that contains a number of overlapping and consecutive phases, disruption in each of which can cause chronic non-healing wounds. In the current study, we investigated the effects of exosomes as paracrine factors released from menstrual blood-derived mesenchymal stem cells (MenSCs) on wound healing process in diabetic mice. The exosomes were isolated from MenSCs conditioned media using ultracentrifugation and were characterized by SEM and western blotting assay. Read More

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http://doi.wiley.com/10.1002/term.2799
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http://dx.doi.org/10.1002/term.2799DOI Listing
January 2019
7 Reads

Extracellular vesicles can act as a potent immunomodulators of human microglial cells.

J Tissue Eng Regen Med 2019 Jan 16. Epub 2019 Jan 16.

Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.

Functional impairments of microglia have been recently associated with several neurological conditions. Therefore, modulation of anti-inflammatory and phagocytic properties of microglial cells could represent a novel therapeutic approach. In the present study, we investigated the effects of extracellular vesicles (EVs) derived from stem cells from the dental pulp of human exfoliated deciduous teeth (SHEDs) on the inflammatory response and functional properties of immortalized human microglial cells. Read More

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http://dx.doi.org/10.1002/term.2810DOI Listing
January 2019

Effect of the small compound TD-198946 on glycosaminoglycan synthesis and transforming growth factor-β3 associated chondrogenesis of human synovium-derived stem cells in vitro.

J Tissue Eng Regen Med 2019 Jan 16. Epub 2019 Jan 16.

Osaka University Graduate School of Medicine, Orthopaedic surgery, Suita, Osaka, Japan.

As an alternative to chondrocytes-based cartilage repair, stem cell-based therapies have been investigated. Specifically, human synovium-derived stem cells (hSSCs) are a promising cell source based on their highly capacities for chondrogenesis, but some methodological improvements are still required towards optimal cartilage regeneration. Recently, a small compound, TD-198946, was reported to promote chondrogenesis of several stem cells, but the effect on hSSCs is still unknown. Read More

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http://dx.doi.org/10.1002/term.2795DOI Listing
January 2019

Effect of donor variation on osteogenesis and vasculogenesis in hydrogel cocultures.

J Tissue Eng Regen Med 2019 Jan 16. Epub 2019 Jan 16.

Department of Oral and Maxillofacial Surgery and Special Dental Care, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.

To introduce a functional vascular network into tissue-engineered bone equivalents, human endothelial colony forming cells (ECFCs) and multipotent mesenchymal stromal cells (MSCs) can be cocultured. Here, we studied the impact of donor variation of human bone marrow-derived MSCs and cord blood-derived ECFCs on vasculogenesis and osteogenesis using a 3D in vitro coculture model. Further, to make the step towards cocultures consisting of cells derived from a single donor, we tested how induced pluripotent stem cell (iPSC)-derived human endothelial cells (iECs) performed in coculture models. Read More

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http://dx.doi.org/10.1002/term.2807DOI Listing
January 2019

Cryopreserved H O -preconditioned human adipose-derived stem cells exhibit fast post-thaw recovery and enhanced bioactivity against oxidative stress.

J Tissue Eng Regen Med 2019 Jan 15. Epub 2019 Jan 15.

Department of Surgery, Radiology and Physical Medicine, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

Human adipose-derived stem cells (ASCs), despite being one of the most attractive cell populations for tissue engineering and regenerative medicine, currently have certain limitations that reduce their therapeutic efficacy. One of the most serious problems is the poor engraftment of cryopreserved ASCs at injured tissue, attributed to the diminished biological activity of ASCs immediately post-thaw and their poor survival under harsh conditions of oxidative stress. Seeking to address these issues, we have developed a hormetic strategy to preadapt human ASCs to oxidative stress based on a new hydrogen peroxide preconditioning procedure, resulting in cells we call HC016. Read More

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http://dx.doi.org/10.1002/term.2797DOI Listing
January 2019

Developing palatal bone using human mesenchymal stem cell and stem cells from exfoliated deciduous teeth cell sheets.

J Tissue Eng Regen Med 2019 Jan 15. Epub 2019 Jan 15.

Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.

Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only aesthetic but also functional problems with speech, eating, and breathing. Bone grafts using autologous cancellous bone have been a standard treatment to repair the hard tissue defect in cleft palates. Read More

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http://doi.wiley.com/10.1002/term.2811
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http://dx.doi.org/10.1002/term.2811DOI Listing
January 2019
3 Reads

Conditioned media serived from mesenchymal stem cell cultures: The next generation for regenerative medicine.

J Tissue Eng Regen Med 2019 Jan 15. Epub 2019 Jan 15.

Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.

Recent studies suggest that the main driving force behind the therapeutic activity observed in mesenchymal stem cells (MSCs) are the paracrine factors secreted by these cells. These biomolecules also trigger anti-apoptotic events to prevent further degeneration of the diseased organ through paracrine signalling mechanisms. In comparison to the normal physiological conditions, an increased paracrine gradient is observed within the peripheral system of diseased organs which enhances the migration of tissue specific MSCs towards the site of infection or injury to promote healing. Read More

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http://dx.doi.org/10.1002/term.2806DOI Listing
January 2019

Gas-foamed poly (lactide-co-glycolide) and poly (lactide-co-glycolide) with bioactive glass fibers demonstrate insufficient bone repair in lapine osteochondral defects.

J Tissue Eng Regen Med 2019 Jan 15. Epub 2019 Jan 15.

Department of Orthopaedics and Traumatology, Clinicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland.

Deep osteochondral defects may leave voids in the subchondral bone, increasing the risk of joint structure collapse. To ensure a stable foundation for the cartilage repair, bone grafts can be used for filling these defects. Poly (lactide-co-glycolide) (PLGA) is a biodegradable material that improves bone healing and supports bone matrix deposition. Read More

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http://doi.wiley.com/10.1002/term.2801
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http://dx.doi.org/10.1002/term.2801DOI Listing
January 2019
4 Reads

A simple, static preservation system for shipping retinal pigment epithelium cell sheets.

J Tissue Eng Regen Med 2019 Jan 15. Epub 2019 Jan 15.

Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan.

The ability to move cells and tissues from bench to bedside is an essential aspect of regenerative medicine. In this study, we propose a simple, static shipping system to deliver tissue-engineered cell sheets. Notably, this system is electronic-device-free and simplified to minimize the number of packing and opening steps involved. Read More

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http://dx.doi.org/10.1002/term.2805DOI Listing
January 2019
1 Read

Attenuation of osteoarthritis progression in mice following intra-articular administration of simvastatin-conjugated gelatin hydrogel.

J Tissue Eng Regen Med 2019 Jan 15. Epub 2019 Jan 15.

Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.

Simvastatin is clinically used for the treatment of hypercholesterolaemia. This study investigated the effects of the intra-articular administration of a simvastatin-conjugated gelatin hydrogel on osteoarthritis (OA) progression in a murine model. The medial meniscus of the knee joint was de-stabilized to induce OA in 10-week-old mice. Read More

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http://dx.doi.org/10.1002/term.2804DOI Listing
January 2019
15 Reads

Hyaline cartilage next generation implants from Adipose Tissue Derived Mesenchymal Stem Cells: Comparative study on 3D-Printed Polycaprolactone scaffold patterns.

J Tissue Eng Regen Med 2019 Jan 13. Epub 2019 Jan 13.

Department of Physiology and Pharmacology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki (A.U.Th), Thessaloniki, Greece.

We used additive manufacturing to fabricate 3D printed polycaprolactone (PCL) scaffolds of different geometry topologies and porosities. We present a comparative analysis of hyaline cartilage development from Adipose Tissue Derived Mesenchymal Stem Cells (ADMSCs) on three different, newly designed scaffold geometry patterns. The first scaffold design (MESO), was based on a rectilinear layer pattern. Read More

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http://dx.doi.org/10.1002/term.2798DOI Listing
January 2019
116 Reads

DNMT inhibitors effectively induce gene expression changes suggestive of cardiomyogenic differentiation of human amniotic fluid-derived mesenchymal stem cells via chromatin remodeling.

J Tissue Eng Regen Med 2019 Jan 13. Epub 2019 Jan 13.

Institute of Biochemistry, Life Sciences Center, Vilnius University, Lithuania.

Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) are a new potential stem cell source for cell therapy and regenerative medicine. These are fetal mesenchymal stem cells with multi-lineage differentiation potential found in amniotic fluid. The aim of the present study was to evaluate in vitro differentiation initiation of AF-MSCs into cardiac progenitors upon application of inhibitors of DNA methyltransferases (DNMT), such as Decitabine (5-aza-2'-deoxycytidine) and Zebularine. Read More

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http://dx.doi.org/10.1002/term.2800DOI Listing
January 2019

Prevalence of CAM-deformity and its influence on therapy success in patients with osteonecrosis of the femoral head.

J Tissue Eng Regen Med 2019 Jan 13. Epub 2019 Jan 13.

Department of Orthopaedics & Traumatology, University of Duisburg-Essen, Essen, Germany.

Osteonecrosis of the femoral head (ONFH) and CAM-type femoroacetabular impingement (FAI) present two different pathologies of the hip joint. The aim of this study is to describe the prevalence of CAM-FAI in a collective of ONFH hips and to evaluate its influence on ONFH therapy outcome. A cohort of 86 ONFH hips with a mean follow-up of 46. Read More

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http://dx.doi.org/10.1002/term.2794DOI Listing
January 2019
1 Read

Bundling of axons through a capillary alginate gel enhances the detection of axonal action potentials using microelectrode arrays.

J Tissue Eng Regen Med 2019 Jan 13. Epub 2019 Jan 13.

Department of Medical Education, College of Medicine, University of Central Florida, Orlando, FL, USA.

Microelectrode arrays (MEAs) have become important tools in high throughput assessment of neuronal activity. However, geometric and electrical constraints largely limit their ability to detect action potentials to the neuronal soma. Enhancing the resolution of these systems to detect axonal action potentials has proved both challenging and complex. Read More

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http://dx.doi.org/10.1002/term.2793DOI Listing
January 2019

Calorie restriction enhances adult mouse lung stem cells function and reverses several ageing-induced changes.

J Tissue Eng Regen Med 2018 Dec 18. Epub 2018 Dec 18.

Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo, Japan.

Ageing is associated with decreased lung function and an increased incidence of lung infections. Several studies have suggested that long-term calorie restriction (CR) promotes health and longevity and results in the reduced risk of several diseases. The effect of CR is thought to be through improving the function of tissue stem cells. Read More

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http://dx.doi.org/10.1002/term.2792DOI Listing
December 2018

Translating the application of transforming growth factor-β1, chondroitinase-ABC, and lysyl oxidase-like 2 for mechanically robust tissue-engineered human neocartilage.

J Tissue Eng Regen Med 2018 Dec 17. Epub 2018 Dec 17.

Department of Biomedical Engineering, University of California, Irvine, Irvine, California.

Strategies to overcome the limited availability of human articular chondrocytes and their tendency to dedifferentiate during expansion are required to advance their clinical use and to engineer functional cartilage on par with native articular cartilage. This work sought to determine whether a biochemical factor (transforming growth factor-β1 [T]), a biophysical agent (chondroitinase-ABC [C]), and a collagen crosslinking enzyme (lysyl oxidase-like 2 [L]) are efficacious in forming three-dimensional human neocartilage from expanded human articular chondrocytes. Among the treatment regimens, the combination of the three stimuli (TCL treatment) led to the most robust glycosaminoglycan content, total collagen content, and type II collagen production. Read More

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http://dx.doi.org/10.1002/term.2791DOI Listing
December 2018
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A computational simulation of cyclic stretch of an individual stem cell using a nonlinear model.

J Tissue Eng Regen Med 2018 Dec 17. Epub 2018 Dec 17.

Division of Biomedical Engineering, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.

Many experiments have shown that mechanical stimuli like cyclic strains might be helpful in stem cell differentiation. To maximize such differentiations efficiency, it is imperative to detect the cellular mechanical responses to these stimuli. The purpose of this research was to show that a newly presented hyper-viscoelastic model could correctly predict the level of stresses required to obtain a different response from a single mesenchymal stem cell cultured in a fibrin hydrogel block under a 10% cyclic strain at a frequency of 1 Hz, employing finite element method. Read More

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http://doi.wiley.com/10.1002/term.2790
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http://dx.doi.org/10.1002/term.2790DOI Listing
December 2018
6 Reads

Packaging functionally important plasma proteins into the α-granules of human-induced pluripotent stem cell-derived megakaryocytes.

J Tissue Eng Regen Med 2018 Dec 17. Epub 2018 Dec 17.

Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin.

The contents of platelet α-granules arrive via a number of pathways; some are synthesized by megakaryocytes (MKs), for example, von Willebrand factor (VWF), whereas others are endocytosed from plasma, for example, fibrinogen (Fgn) and factor V (FV). Currently, almost all in vitro-induced pluripotent stem cell (iPSC)-derived MKs are generated under serum-free conditions, and their α-granule cargoes lack components that would normally be taken up from plasma during the course of megakaryopoiesis. How this might affect the ability of in vitro-derived platelets to contribute fully to haemostasis is not known. Read More

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http://dx.doi.org/10.1002/term.2785DOI Listing
December 2018

Platelet-derived growth factor stabilises vascularisation in collagen-glycosaminoglycan scaffolds in vitro.

J Tissue Eng Regen Med 2018 Dec 16. Epub 2018 Dec 16.

Kearney Lab, Department of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland.

Collagen-glycosaminoglycan (CG) scaffolds have been widely developed for a range of regenerative medicine applications. To enhance their efficacy, CG scaffolds have previously been prevascularised in vitro using human umbilical vein endothelial cells and human mesenchymal stromal cells (hMSCs); however, at later timepoints, a regression of vascularisation is observed. This is undesirable for longer preculture periods (e. Read More

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http://dx.doi.org/10.1002/term.2789DOI Listing
December 2018
4 Reads

Porcine-derived biomaterials in tissue engineering and reconstructive surgery: Considerations and alternatives in Muslim patients.

J Tissue Eng Regen Med 2018 Dec 16. Epub 2018 Dec 16.

Department of Plastic Surgery, Hand Surgery and Burn Center, Faculty of Medicine, RWTH University Hospital, Aachen, Germany.

During the last three decades, tissue engineering and reconstructive surgery have become standard therapeutic options in the world of medicine. Several biomaterials, either alone or in combination with cultured cellular products, have been introduced to compensate for the scarcity of autologous donor tissue or to improve healing in a variety of surgical specialties, for example, abdominal/visceral surgery, plastic surgery, and cardiovascular surgery. Many of these biomaterials are of porcine origin. Read More

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http://dx.doi.org/10.1002/term.2788DOI Listing
December 2018
1 Read

Characterization of plasma fibronectin for migration, proliferation and differentiation on human articular chondrocytes.

J Tissue Eng Regen Med 2018 Dec 14. Epub 2018 Dec 14.

TransTissue Technologies GmbH, Berlin.

Plasma fibronectin (pFN) plays a crucial role in wound healing by binding to integrins and inducing cell migration. It is known to induce the migration and proliferation of mesenchymal progenitor in vitro, which play a key role during microfracture in cartilage repair. Endogenous chondrocytes from the native cartilage of the defect rim might aid in cartilage repair. Read More

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http://dx.doi.org/10.1002/term.2787DOI Listing
December 2018
1 Read

Optogenetic control of iPS cell-derived neurons in 2D and 3D culture systems using channelrhodopsin-2 expression driven by the synapsin-1 and calcium-calmodulin kinase II promoters.

J Tissue Eng Regen Med 2018 Dec 14. Epub 2018 Dec 14.

Department of Oncology, Old Road Campus Research Building, University of Oxford, Oxford, UK.

Development of an optogenetically controllable human neural network model in three-dimensional (3D) cultures can provide an investigative system that is more physiologically relevant and better able to mimic aspects of human brain function. Light-sensitive neurons were generated by transducing channelrhodopsin-2 (ChR2) into human induced pluripotent stem cell (hiPSC) derived neural progenitor cells (Axol) using lentiviruses and cell-type specific promoters. A mixed population of human iPSC-derived cortical neurons, astrocytes and progenitor cells were obtained (Axol-ChR2) upon neural differentiation. Read More

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http://dx.doi.org/10.1002/term.2786DOI Listing
December 2018

Macromolecular crowding as a means to assess the effectiveness of chondrogenic media.

J Tissue Eng Regen Med 2018 Dec 14. Epub 2018 Dec 14.

Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland.

Chondrocyte-based tissue engineering requires in vitro cell expansion, which is associated with phenotypic losses, decrease in Collagen Type II synthesis and increase in Collagen Type I synthesis. Another major obstacle in clinical translation of chondrocyte-based therapies is the lack of extracellular matrix (ECM) in the engineered cartilage substitutes. Various research and commercially available media claim that they can maintain chondrogenic phenotype, whereas macromolecular crowding (MMC) has been shown to increase tissue-specific ECM deposition and maintain cell phenotype in vitro. Read More

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http://dx.doi.org/10.1002/term.2783DOI Listing
December 2018

RGD-functionalized polyurethane scaffolds promote umbilical cord blood mesenchymal stem cell expansion and osteogenic differentiation.

J Tissue Eng Regen Med 2018 Dec 11. Epub 2018 Dec 11.

Biological Systems Engineering Laboratory, Department of Chemical Engineering and Chemical Technology, Imperial College London, London, UK.

Biomimetic materials are essential for the production of clinically relevant bone grafts for bone tissue engineering applications. Their ability to modulate stem cell proliferation and differentiation can be used to harness the regenerative potential of those cells and optimize the efficiency of engineered bone grafts. The arginyl-glycyl-aspartic acid (RGD) peptide has been recognized as the adhesion motif of various extracellular matrix proteins and can affect stem cell behaviour in biomaterials. Read More

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http://dx.doi.org/10.1002/term.2784DOI Listing
December 2018
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Quantifying proliferative and surface marker heterogeneity in colony-founding connective tissue progenitors and their progeny using time-lapse microscopy.

J Tissue Eng Regen Med 2018 Dec 7. Epub 2018 Dec 7.

Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio, USA.

Connective tissue progenitors (CTPs) are defined as the heterogeneous population of tissue-resident stem and progenitor cells that are capable of proliferating and differentiating into connective tissue phenotypes. The prevalence and variation in clonal progeny of CTPs can be characterized using a colony formation assay. However, colony assays do not directly assess the characteristics of the colony-founding CTP. Read More

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http://dx.doi.org/10.1002/term.2782DOI Listing
December 2018

Persistence of fluorescent nanoparticle-labelled bone marrow mesenchymal stem cells in vitro and after intra-articular injection.

J Tissue Eng Regen Med 2018 Dec 7. Epub 2018 Dec 7.

Department of Large Animal Clinical Sciences, Texas A&M University, College Station, Texas, USA.

Mesenchymal stem cells (MSCs) improve the osteoarthritis condition, but the fate of MSCs after intra-articular injection is unclear. We used fluorescent nanoparticles (quantum dots [QDs]) to track equine MSCs (QD-labelled MSCs [QD-MSCs]) in vivo after intra-articular injection into normal and osteoarthritic joints. One week after injection of QD-MSCs, unlabelled MSCs, or vehicle, we determined the presence of QD-MSCs in synovium and articular cartilage histologically. Read More

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http://dx.doi.org/10.1002/term.2781DOI Listing
December 2018
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Gap closure of different shape wounds: In vitro and in vivo experimental models in the presence of engineered protein adhesive hydrogel.

J Tissue Eng Regen Med 2018 Dec 7. Epub 2018 Dec 7.

Biological Materials Laboratory, Microbiology Division, CSIR-CLRI (Central Leather Reasearch Institute), Adyar, Chennai, India.

The present study emphasizes the role of engineered protein (gallic acid engineered gelatin [GEG]) on the closure of wound gaps of different shapes assessed under in vitro (fibroblast cell line) and in vivo (rat) experimental models. Circular, triangle, rectangle, and square are the shapes selected for the study. Intending engineered protein (GEG) augments the cell migration in rectangle and triangle shapes and reduces the gap space significantly compared with circular and square shapes. Read More

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http://dx.doi.org/10.1002/term.2779DOI Listing
December 2018

Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds.

J Tissue Eng Regen Med 2018 Dec 7. Epub 2018 Dec 7.

Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.

Recent studies have demonstrated that surface characteristics, porosity, and mechanical strength of three-dimensional 45S5-type bioactive glass (BG)-based scaffolds are directly correlated with osteogenic properties. Three-dimensional BG-based scaffolds obtained from maritime natural sponges (MNSs) as sacrificial templates exhibit the required morphological properties; however, no in vivo data about the osteogenic features are available. In this study, uncoated (Group A) and gelatin-coated (Group B) crystalline MNS-obtained BG-based scaffolds were evaluated mechanically and seeded with human mesenchymal stem cells prior to subcutaneous implantation in immunodeficient mice. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2780
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http://dx.doi.org/10.1002/term.2780DOI Listing
December 2018
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Fibrin glue displays promising in vitro characteristics as a potential carrier of adipose progenitor cells for tissue regeneration.

J Tissue Eng Regen Med 2018 Dec 4. Epub 2018 Dec 4.

Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Munich University Hospital, Ludwig Maximilians University, Munich, Germany.

Adipose-derived multipotent stem/progenitor cells (ASPCs) were shown to be ideal candidates for cell-based regenerative therapies. Yet, despite their huge potential, successful clinical applications are still rare. It was suggested that the efficacy of ASPCs at the recipient site depends on the vehicle of cell delivery. Read More

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http://doi.wiley.com/10.1002/term.2778
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http://dx.doi.org/10.1002/term.2778DOI Listing
December 2018
1 Read
5.199 Impact Factor

Enterococcus faecalis sir2-like gene enhances aerobic metabolism of themselves and mitochondrial respiration of mammal cells to bring about improving metabolic syndrome through the PGC-1α pathway.

J Tissue Eng Regen Med 2018 Dec 4. Epub 2018 Dec 4.

Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou, China.

Recent studies showed that probiotics could improve metabolic syndrome, making the identification of factors affecting metabolic control more important than ever. The mammalian sirtuin protein family has received much attention for its regulatory role, especially in various mitochondrial ATP, glucose, and lipid metabolic pathways. However, compared with the mammalian sirtuin protein family, the function of prokaryotic sir2 protein is much less known. Read More

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http://dx.doi.org/10.1002/term.2775DOI Listing
December 2018

A collagen-coated sponge silk scaffold for functional meniscus regeneration.

J Tissue Eng Regen Med 2018 Nov 28. Epub 2018 Nov 28.

Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China.

Tissue engineering is a promising solution for meniscal regeneration after meniscectomy. However, in situ reconstruction still poses a formidable challenge due to multifunctional roles of the meniscus in the knee. In this study, we fabricate a silk sponge from 9% (w/v) silk fibroin solution through freeze drying and then coat its internal space and external surface with collagen sponge. Read More

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http://dx.doi.org/10.1002/term.2777DOI Listing
November 2018
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Reduced sirtuin 1/adenosine monophosphate-activated protein kinase in amyotrophic lateral sclerosis patient-derived mesenchymal stem cells can be restored by resveratrol.

J Tissue Eng Regen Med 2019 Jan 10;13(1):110-115. Epub 2018 Dec 10.

Department of Biology, College of Natural Science, Chosun University, Gwangju, Korea.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease affecting motor neuron system. Our previous study has shown that bone marrow-mesenchymal stem cells (BM-MSCs) from ALS patients have functional limitations in releasing neurotrophic factors and exhibit the senescence phenotype. In this study, we examined sirtuin 1/adenosine monophosphate-activated protein kinase (SIRT1/AMPK) activities and identified significant decreases in the ALS-MSCs compared with normal healthy control originated BM-MSCs. Read More

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http://doi.wiley.com/10.1002/term.2776
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http://dx.doi.org/10.1002/term.2776DOI Listing
January 2019
16 Reads
5.199 Impact Factor

Subnormothermic short-term cultivation improves the vascularization capacity of adipose tissue-derived microvascular fragments.

J Tissue Eng Regen Med 2018 Nov 23. Epub 2018 Nov 23.

Institute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, Germany.

Adipose tissue-derived microvascular fragments (ad-MVFs) are promising vascularization units for tissue engineering. In this study, we analysed the effects of normothermic (37°C) and subnormothermic (20°C) short-term cultivation on their viability and network forming capacity. Ad-MVFs from green fluorescent protein (GFP) and GFP C57BL/6 mice were cultivated for 24 hr at 37°C or 20°C. Read More

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http://dx.doi.org/10.1002/term.2774DOI Listing
November 2018
1 Read

Tissue-engineered nanoclay-based 3D in vitro breast cancer model for studying breast cancer metastasis to bone.

J Tissue Eng Regen Med 2018 Nov 22. Epub 2018 Nov 22.

Department of Civil and Environmental Engineering, CIE 201, NDSU, Fargo, North Dakota, United States.

Breast cancer (BrCa) preferentially spreads to bone and colonises within the bone marrow to cause bone metastases. To improve the outcome of patients with BrCa bone metastasis, we need to understand better the mechanisms underlying bone metastasis. Researchers have relied heavily upon in vivo xenografts due to limited availability of human bone metastasis samples. Read More

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http://dx.doi.org/10.1002/term.2773DOI Listing
November 2018
6 Reads