1,775 results match your criteria Journal of tissue engineering and regenerative medicine[Journal]


Proteomics and N-Glycoproteomics analysis of an extracellular matrix-based scaffold-human treated dentin matrix.

J Tissue Eng Regen Med 2019 Apr 12. Epub 2019 Apr 12.

College of Stomatology, Chongqing Medical University, Chongqing, China.

Extracellular matrix (ECM)-based biomaterials developed from mammalian tissues have been successfully used in preclinical and clinical tissue engineering applications. We have previously reported about the applicability of dentin-based scaffold, treated dentin matrix (TDM), for tooth root regeneration. However, TDM protein composition has not been characterized. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2866
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http://dx.doi.org/10.1002/term.2866DOI Listing
April 2019
2 Reads
5.199 Impact Factor

Corrigendum.

Authors:

J Tissue Eng Regen Med 2019 Apr;13(4):704

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http://dx.doi.org/10.1002/term.2850DOI Listing

Developing A Synthetic Composite Membrane For Cleft Palate Repair.

J Tissue Eng Regen Med 2019 Apr 11. Epub 2019 Apr 11.

Department of Materials Science &Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK.

An oronasal fistula is a passage between the oral and nasal cavity. Currently, surgical procedures use mucosal flaps or collagen grafts to make a barrier between oral and nasal cavities. Our aim was to develop a cell-free synthetic repair material for closure of nasal fistulas. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2867
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http://dx.doi.org/10.1002/term.2867DOI Listing
April 2019
4 Reads
5.199 Impact Factor

Human saliva stimulates skin and oral wound healing in vitro.

J Tissue Eng Regen Med 2019 Apr 9. Epub 2019 Apr 9.

Department of Molecular Cell Biology and Immunology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, The Netherlands.

Despite continuous exposure to environmental pathogens, injured mucosa within the oral cavity heals faster and almost scar free compared to skin. Saliva is thought to be one of the main contributing factors. Saliva may possibly also stimulate skin wound healing. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2865
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http://dx.doi.org/10.1002/term.2865DOI Listing
April 2019
12 Reads

Effects of Hydrostatic Pressure and Deviatoric Stress on Human Articular Chondrocytes for Designing Neo-Cartilage Construct.

J Tissue Eng Regen Med 2019 Apr 9. Epub 2019 Apr 9.

Department of Orthopedic Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Autologous chondrocyte implantation is a promising therapy for the treatment of the articular cartilage defects. Recently, we have developed a three-dimensional chondrocyte construct manufactured with a collagen gel/sponge scaffold and cyclic hydrostatic pressure. However, the roles of various mechanical stresses, specifically hydrostatic pressure and deviatoric stress, as well as post-stress loading, were unclear on metabolic function in chondrocytes. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2863
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http://dx.doi.org/10.1002/term.2863DOI Listing
April 2019
10 Reads

Timing of extracorporeal shock wave therapy in rabbits with carpal tunnel syndrome.

J Tissue Eng Regen Med 2019 Apr 9. Epub 2019 Apr 9.

Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul, South Korea.

The purpose of this study is to evaluate the effect of extracorporeal shock wave therapy (ESWT) according to treatment timing in rabbits with 10% dextrose-induced carpal tunnel syndrome (CTS); 0.1-ml 10% dextrose solution was injected under ultrasound guidance twice weekly to the left forepaw subsynovial connective tissue (SSCT) within the carpal tunnel of 36 New Zealand white rabbits to induce CTS. The rabbits were randomly allocated into four groups: G1-S (sham ESWT), G2-E4 (ESWT at 4 weeks), G3-E8 (at 8 weeks), and G4-E16 (at 16 weeks). Read More

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http://dx.doi.org/10.1002/term.2862DOI Listing
April 2019
2 Reads

Vitrification and Storage of Oral Mucosa Epithelial Cell Sheets.

J Tissue Eng Regen Med 2019 Apr 9. Epub 2019 Apr 9.

Emmaus Medical, Inc., Torrance, CA, USA.

Shipping time and shipping delays might affect the quality of the stem cells based engineered "organs". In our laboratory, we have developed a limbal stem cell deficient (LSCD) rabbit model. To reverse the LSCD, we cultured oral mucosal epithelial cells for 2-3 weeks and engineered cultured autologous oral mucosa epithelial cell sheets (CAOMECS), which were grafted on the LSCD cornea. Read More

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http://dx.doi.org/10.1002/term.2864DOI Listing
April 2019
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Therapeutic effect of long-interval repeated intravenous administration of human umbilical cord blood-derived mesenchymal stem cells in DBA/1 mice with collagen-induced arthritis.

J Tissue Eng Regen Med 2019 Apr 8. Epub 2019 Apr 8.

Stem Cell and Regenerative Bioengineering Institute, Kangstem Biotech Co., Ltd., Biotechnology Center, Seoul National University, Seoul, South Korea.

Rheumatoid arthritis (RA) is a common inflammatory chronic disease. It has been reported that MSCs have the effect of immune suppression in CIA mice model. However, in vivo the therapeutic effect from the long-interval repeated intravenous administration of hUCB-MSCs had not been investigated in CIA mice model. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2861
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http://dx.doi.org/10.1002/term.2861DOI Listing
April 2019
3 Reads
5.199 Impact Factor

Tuning the Biomimetic Behavior of Scaffolds for Regenerative Medicine Through Surface Modifications.

J Tissue Eng Regen Med 2019 Apr 4. Epub 2019 Apr 4.

School of Chemical, Biological, and Materials Engineering, The University of Oklahoma, Norman, Oklahoma.

Tissue engineering and regenerative medicine rely extensively on biomaterial scaffolds to support cell adhesion, proliferation, and differentiation physically and chemically in vitro and in vivo. Changes to the surface characteristics of the scaffolds have the greatest impact on cell response. Here we discuss five dominant surface modification approaches used to biomimetically improve the most common scaffolds for tissue engineering, those based on aliphatic polyesters. Read More

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http://dx.doi.org/10.1002/term.2859DOI Listing
April 2019
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Peptide Nanostructures on Nanofibers for Peripheral Nerve Regeneration.

J Tissue Eng Regen Med 2019 Apr 4. Epub 2019 Apr 4.

Centre of Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA Deemed University, Tirumalaisamudram, Thanjavur, Tamil Nadu, India.

Self-assembled peptide nanofibrous scaffolds [SAPNF] with designer sequences similar to neurite growth promoting molecules enhance the differentiation of neural stem cells. However, SAPNF lack the required mechanical strength to suffice to bridge long critical-sized peripheral nerve defects. Hence, there is a demand for a potential neural substrate which could be biomimetic coupled with bioactive nanostructures to regrow the denuded axons towards the distal end. Read More

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http://dx.doi.org/10.1002/term.2860DOI Listing
April 2019
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Characterization of human skin equivalents developed at body's core and surface temperatures.

J Tissue Eng Regen Med 2019 Apr 3. Epub 2019 Apr 3.

Department of Dermatology, Leiden University Medical Centre, the Netherlands.

Human skin equivalents (HSEs) are in vitro developed three-dimensional models resembling native human skin (NHS) to a high extent. However, the epidermal lipid biosynthesis, barrier lipid composition and organization are altered, leading to an elevated diffusion rate of therapeutic molecules. The altered lipid barrier formation in HSEs may be induced by standardized culture conditions, including a culture temperature of 37°C, which is dissimilar to skin surface temperature. Read More

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http://dx.doi.org/10.1002/term.2858DOI Listing
April 2019
6 Reads

Human Gingival Mesenchymal Stem Cells (GMSCs) pre-treated with vesicular Moringin nanostructures as a new therapeutic approach in a mouse model of Spinal Cord Injury.

J Tissue Eng Regen Med 2019 Apr 3. Epub 2019 Apr 3.

IRCCS Centro Neurolesi Bonino-Pulejo, Contrada Casazza, Messina, Italy.

Spinal cord injury (SCI) is a neurological disorder that arises from a primary acute mechanical lesion, followed by a pathophysiological cascade of events that leads to further spinal cord tissue damage. Several preclinical and clinical studies have highlighted the ability of stem cell therapy to improve long-term functional recovery in SCI. Previously, we demonstrated that Moringin (MOR) treatment accelerates the differentiation process in mesenchymal stem cells inducing an early up-regulation of neural development associated genes. Read More

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http://dx.doi.org/10.1002/term.2857DOI Listing
April 2019
2 Reads

Biodegrading, highly porous elastomeric graft regenerates muscular and innervated carotid artery--comparative study with vein graft.

J Tissue Eng Regen Med 2019 Apr 3. Epub 2019 Apr 3.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Oral & Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.

This study aims to investigate the superiorities of fast degrading, elastomeric PGS/PCL grafts over autologous vein grafts in the reconstruction of carotid artery, thus provide more suitable vascular grafts for carotid artery replacement. We fabricated small arterial grafts from microporous tubes of poly (glycerol sebacate) (PGS) reinforced with polycaprolactone (PCL) nanofibers on the outer surface. As control, autologous jugular veins were harvested as vein grafts. Read More

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http://dx.doi.org/10.1002/term.2856DOI Listing
April 2019
2 Reads

Combination of cord blood-derived human hepatic progenitors and hepatogenic factors strongly improves recovery after acute liver injury in mice through modulation of the Wnt/β-catenin signaling.

J Tissue Eng Regen Med 2019 Apr 3. Epub 2019 Apr 3.

Institute of Translational Pharmacology (IFT), Department of Biomedical Sciences, National Research Council (CNR), Rome, Italy.

Cell therapy represents the promising alternative strategy for end-stage liver disease, and hepatic progenitors are the best candidates. The possibility to maximize the paracrine effects of transplanted cells represents a great potential benefit for cell therapy success. We studied how cell type and microenvironment modulate the Wnt/β-catenin signaling in vitro and in vivo. Read More

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http://dx.doi.org/10.1002/term.2854DOI Listing
April 2019
1 Read

Keratin Scaffolds with Human Adipose Stem Cells: Physical and Biological Effects toward Wound Healing.

J Tissue Eng Regen Med 2019 Apr 2. Epub 2019 Apr 2.

Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei, Taiwan.

Keratin, a natural biomaterial derived from wool or human hair, has the intrinsic ability to interact with different types of cells and the potential to serve as a controllable extracellular matrix which can be used a scaffold for tissue engineering. In this study, we demonstrated a simple and fast technique to construct 3D keratin scaffolds for accelerated wound healing using a lyophilization method based on extraction of keratin from human hair. The physical properties of the keratin scaffold s such as water uptake, pore size and porosity can be adjusted by changing the protein concentrations during the fabrication process. Read More

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http://dx.doi.org/10.1002/term.2855DOI Listing
April 2019
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TGF β1 ACTIVATED TYPE 2 DENDRITIC CELLS PROMOTE WOUND HEALING AND INDUCE FIBROBLASTS TO EXPRESS TENASCIN C FOLLOWING CORNEAL FULL-THICKNESS HYDROGEL TRANSPLANTATION.

J Tissue Eng Regen Med 2019 Apr 1. Epub 2019 Apr 1.

School of Medicine and Dentistry, Section of Immunology, Inflammation and Infection, Institute of Medical Sciences, Division of Applied Medicine, University of Aberdeen, Scotland, UK.

We showed previously that 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide hydrochloride (EDC) crosslinked recombinant human collagen III (RHCIII) hydrogels promoted stable regeneration of the human cornea (continued nerve and stromal cell repopulation) for over four years. However, as EDC crosslinking kinetics were difficult to control we additionally tested a sterically bulky carbodiimide. Here, we compared the effects of two carbodiimide crosslinkers - bulky, aromatic CMC, and non-bulky EDC - in a mouse corneal graft model. Read More

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http://dx.doi.org/10.1002/term.2853DOI Listing
April 2019
1 Read

The ideal way to design clinical trials and establishment of evidence for human cellular and tissue-based products in Japan.

Authors:
Yoshiki Sawa

J Tissue Eng Regen Med 2019 Apr 1. Epub 2019 Apr 1.

Division of Cardiovascular Surgery, Department of Surgery, Osaka University Graduate School of Medicine, Suita, Japan.

In Japan, the Pharmaceuticals and Medical Devices (PMD) Act established in 2014 included an additional chapter dedicated to frameworks for human cellular and tissue-based products. To further evaluate the product considered to have "likely to predict efficacy" at the time of receiving "conditional and time-limited marketing authorization," a system has been introduced to determine whether the product is eligible for "full marketing authorization" through statistical "confirmation of the efficacy" in a postmarketing surveillance study using a registry. A movement similar to this regulation has been seen among Western nations. Read More

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http://dx.doi.org/10.1002/term.2852DOI Listing
April 2019
1 Read

Recent advances in 3D bioprinting of stem cells.

J Tissue Eng Regen Med 2019 Mar 13. Epub 2019 Mar 13.

Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Telangana, India.

In spite of being a new field, 3D bioprinting has undergone rapid growth in the recent years. Bioprinting methods offer a unique opportunity for stem cell distribution, positioning, and differentiation at the microscale to make the differentiated architecture of any tissue while maintaining precision and control over the cellular microenvironment. Bioprinting introduces a wide array of approaches to modify stem cell fate. Read More

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http://dx.doi.org/10.1002/term.2839DOI Listing

Reduction of BMP6-induced bone formation by calcium phosphate in wild-type compared with nude mice.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Tissue Engineering Laboratory, Skeletal Biology and Engineering Research Center, Katholieke Universiteit Leuven, Leuven, Belgium.

Nude mice have been extensively used to investigate the potency of tissue engineering strategies for bone repair. However, the contribution of pro-inflammatory and proregenerative stimuli of the host for the process of new bone formation and integration remains poorly understood. In this study, ectopic bone formation was investigated in nude (Nu) versus wild-type (WT) mice. Read More

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http://dx.doi.org/10.1002/term.2837DOI Listing
February 2019

Substrate elasticity induces quiescence and promotes neurogenesis of primary neural stem cells - a biophysical in vitro model of the physiological cerebral milieu.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Department of Neurology, University Hospital Cologne, Cologne, Germany.

In the brain, neural stem cells (NSC) are tightly regulated by external signals and biophysical cues mediated by the local microenvironment or "niche". In particular, the influence of tissue elasticity, known to fundamentally affect the function of various cell types in the body, on NSC remains poorly understood. We, accordingly, aimed to characterize the effects of elastic substrates on critical NSC functions. Read More

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http://dx.doi.org/10.1002/term.2838DOI Listing
February 2019
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Advances in three-dimensional bioprinting of bone: Progress and challenges.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Stem Cell Facility (DBT-Centre of Excellence for Stem Cell Research), All India Institute of Medical Sciences (AIIMS), New Delhi, India.

Several attempts have been made to engineer a viable three-dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re-establishing the 3D dynamic micro-environment of the native bone. Read More

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http://doi.wiley.com/10.1002/term.2847
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http://dx.doi.org/10.1002/term.2847DOI Listing
February 2019
7 Reads

Precision cell delivery in biphasic polymer systems enhances growth of keratinocytes in culture and promotes their attachment on acellular dermal matrices.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

School of Biomedical Engineering, Dalhousie University, Halifax, Canada.

Current approaches for precision deposition of cells are not optimized for moist environments or for substrates with complex surface topographic features, for example, the surface of dermal matrices and other biomaterials. To overcome these challenges, an approach is presented that utilizes cell confinement in phase-separating polymer solutions of polyethylene glycol and dextran to precisely deliver keratinocytes in well-defined colonies. Using this approach, keratinocyte colonies are produced with superior viability, proliferative capacity, and barrier formation compared with the same number of cells dispersedly seeded across substrate surfaces. Read More

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http://dx.doi.org/10.1002/term.2845DOI Listing
February 2019

Beta-tricalcium phosphate ceramic triggers fast and robust bone formation by human mesenchymal stem cells.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Laboratory of Regenerative Medicine, Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy.

Due to their osteoconductive and inductive properties, a variety of calcium phosphate (CaP) scaffolds are commonly used in orthopaedics as graft material to heal bone defects. In this study, we have used two CaP scaffolds with different hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ratios (MBCP®; 60/40 and MBCP ®; 20/80) to investigate their intrinsic capacity to favour human bone marrow stem cells (hBMSCs) osteogenic differentiation capacity. We report that MBCP ® showed in in vitro culture model a higher rate of calcium ion release in comparison with MBCP®. Read More

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http://dx.doi.org/10.1002/term.2848DOI Listing
February 2019

A promising therapeutic option for diabetic bladder dysfunction: Adipose tissue-derived stem cells pretreated by defocused low-energy shock wave.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

Adipose tissue-derived stem cells (ADSCs) have shown effectiveness in treating diabetic bladder dysfunction (DBD). In the present study, ADSCs pretreated by defocused low-energy shock wave (DLSW) were first used to achieve better therapeutic effect. ADSCs were treated by DLSW prior to each passage. Read More

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http://dx.doi.org/10.1002/term.2844DOI Listing
February 2019
2 Reads

Analysis of the therapeutic potential of different administration routes and frequencies of human mesenchymal stromal cells in the SOD1 mouse model of amyotrophic lateral sclerosis.

J Tissue Eng Regen Med 2019 Apr 20;13(4):649-663. Epub 2019 Mar 20.

Department of Neurology, Hannover Medical School, Hannover, Germany.

Cellular therapy represents a novel option for the treatment of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS). Its major aim is the generation of a protective environment for degenerating motor neurons. Mesenchymal stromal cells secrete different growth factors and have antiapoptotic and immunomodulatory properties. Read More

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http://dx.doi.org/10.1002/term.2846DOI Listing
April 2019
3 Reads

Rapid treatment of full-thickness skin loss using ovine tendon collagen type I scaffold with skin cells.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Tissue Engineering Centre, UKM Medical Centre, Kuala Lumpur, Malaysia.

The full-thickness skin wound is a common skin complication affecting millions of people worldwide. Delayed treatment of this condition causes the loss of skin function and integrity that could lead to the development of chronic wounds or even death. This study was aimed to develop a rapid wound treatment modality using ovine tendon collagen type I (OTC-I) bio-scaffold with or without noncultured skin cells. Read More

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http://dx.doi.org/10.1002/term.2842DOI Listing
February 2019

CD8+ T Cells are Involved in Early Inflammation Before Macrophages in a Rat Adipose Tissue Engineering Chamber Model.

J Tissue Eng Regen Med 2019 Feb 27. Epub 2019 Feb 27.

Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Adipose tissues regenerated using tissue engineering chambers (TECs) show the potential for applications in reconstruction of soft tissue defects. Previous studies have shown that early inflammation plays a key role in angiogenesis and adipogenesis required for adipose tissue generation. However, the sequence of events of the early inflammatory cascade is unclear. Read More

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http://doi.wiley.com/10.1002/term.2836
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http://dx.doi.org/10.1002/term.2836DOI Listing
February 2019
9 Reads

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea.

J Tissue Eng Regen Med 2019 Feb 26. Epub 2019 Feb 26.

Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Tracheal resection is often performed for malignant tumours, congenital anomalies, inflammatory lesions, and traumatic injuries. There is no consensus on the best approach for the restoration of tracheal functionality in patients with tracheal defects. Artificial grafts made of polypropylene and collagen sponge have been clinically used by our group. Read More

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http://dx.doi.org/10.1002/term.2849DOI Listing
February 2019
1 Read

Adipose tissue-derived extracellular matrix hydrogels as a release platform for secreted paracrine factors.

J Tissue Eng Regen Med 2019 Feb 26. Epub 2019 Feb 26.

Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Fat grafting is an established clinical intervention to promote tissue repair. The role of the fat's extracellular matrix (ECM) in regeneration is largely neglected. We investigated in vitro the use of human adipose tissue-derived ECM hydrogels as release platform for factors secreted by adipose-derived stromal cells (ASCs). Read More

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http://dx.doi.org/10.1002/term.2843DOI Listing
February 2019
2 Reads
5.199 Impact Factor

Collagen sponge scaffolds containing growth factors for the functional regeneration of tracheal epithelium.

J Tissue Eng Regen Med 2019 Feb 26. Epub 2019 Feb 26.

Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Tracheal epithelia have barrier and mucociliary clearance functions that prevent invasion of extraneous particles and infectious materials. Hence, following tracheal reconstructions, functional and morphological regeneration of epithelia is required to prevent respiratory declines and infectious diseases. Although growth factors (GFs) promote the regeneration of tracheal epithelial morphologies, it remains unclear whether tracheal grafts containing GFs are beneficial for regeneration of tracheal epithelial functions. Read More

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http://dx.doi.org/10.1002/term.2835DOI Listing
February 2019
1 Read

Photo-crosslinked gelatin-hyaluronic acid methacrylate hydrogel-committed nucleus pulposus-like differentiation of adipose stromal cells for intervertebral disc repair.

J Tissue Eng Regen Med 2019 Apr 19;13(4):682-693. Epub 2019 Mar 19.

Department of Orthopaedics, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Nucleus pulposus-like differentiation is always the challenge with application of stem cells for intervertebral disc repair. The combination of injectable biomaterials and stem cells may provide a resolution for this problem, as the transmembrane force can affect the intracellular environment through integrin αβ. In this study, we developed a strategy of photo-crosslinked gelatin-hyaluronic acid methacrylate (GelHA) hydrogel to commit the nucleus pulposus-like differentiation of adipose stromal cells (ASCs) for intervertebral disc repair. Read More

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http://dx.doi.org/10.1002/term.2841DOI Listing

Combinatorial tissue engineering partially restores function after spinal cord injury.

J Tissue Eng Regen Med 2019 Feb 26. Epub 2019 Feb 26.

Department of Neurology, Mayo Clinic, Rochester, Minnesota, USA.

Hydrogel scaffolds provide a beneficial microenvironment in transected rat spinal cord. A combinatorial biomaterials-based strategy provided a microenvironment that facilitated regeneration while reducing foreign body reaction to the three-dimensional spinal cord construct. We used poly lactic-co-glycolic acid microspheres to provide sustained release of rapamycin from Schwann cell (SC)-loaded, positively charged oligo-polyethylene glycol fumarate scaffolds. Read More

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http://dx.doi.org/10.1002/term.2840DOI Listing
February 2019
3 Reads

Nanoparticulate mineralized collagen glycosaminoglycan materials directly and indirectly inhibit osteoclastogenesis and osteoclast activation.

J Tissue Eng Regen Med 2019 Feb 25. Epub 2019 Feb 25.

Division of Plastic and Reconstructive Surgery, UCLA David Geffen School of Medicine, Los Angeles, California.

The ability of the extracellular matrix (ECM) to direct cell fate has generated the potential for developing a materials-only strategy for tissue regeneration. Previously, we described a nanoparticulate mineralized collagen glycosaminoglycan (MC-GAG) material that efficiently induced osteogenic differentiation of human mesenchymal stem cells (hMSCs) and calvarial bone healing without exogenous growth factors or progenitor cell expansion. In this work, we evaluated the interactions between MC-GAG and primary human osteoclasts (hOCs). Read More

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http://dx.doi.org/10.1002/term.2834DOI Listing
February 2019

Protective effects of curcumin on radioiodine-induced salivary gland dysfunction in mice.

J Tissue Eng Regen Med 2019 Apr 20;13(4):674-681. Epub 2019 Mar 20.

Translational Research Center, Inha University, Incheon, Republic of Korea.

Radioiodine (RI) treatment is widely used in patients with differentiated thyroid cancer. However, RI exposure often induces salivary gland (SG) dysfunction. In this study, we investigated the effect of curcumin on RI-induced SG dysfunction in mice. Read More

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http://dx.doi.org/10.1002/term.2833DOI Listing
April 2019
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Nanostructured fibrin agarose hydrogel as a novel haemostatic agent.

J Tissue Eng Regen Med 2019 Apr 20;13(4):664-673. Epub 2019 Mar 20.

Instituto de Biomedicina de Sevilla, IBIS (HUVR/CSIC/Universidad de Sevilla, Junta de Andalucía), Spain.

Blood loss remains a major concern during surgery and can increase the morbidity of the intervention. The use of topical haemostatic agents to overcome this issue therefore becomes necessary. Fibrin sealants are promising haemostatic agents due to their capacity to promote coagulation, but their effectiveness and applicability need to be improved. Read More

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http://dx.doi.org/10.1002/term.2831DOI Listing
April 2019
4 Reads

Scaffolding kidney organoids on silk.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine.

End stage kidney disease affects hundreds of thousands of patients in the United States. The therapy of choice is kidney replacement, but availability of organs is limited, and alternative sources of tissue are needed. Generation of new kidney tissue in the laboratory has been made possible through pluripotent cell reprogramming and directed differentiation. Read More

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http://dx.doi.org/10.1002/term.2830DOI Listing
February 2019

Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair in a goat model.

J Tissue Eng Regen Med 2019 Apr 1;13(4):694-703. Epub 2019 Apr 1.

Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Traditional treatment therapies for tracheal stenosis often cause severe post-operative complications. To solve the current difficulties, novel and more suitable long-term treatments are needed. A whole-segment tissue-engineered trachea (TET) representing the native goat trachea was 3D printed using a poly(caprolactone) (PCL) scaffold engineered with autologous auricular cartilage cells. Read More

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http://dx.doi.org/10.1002/term.2828DOI Listing
April 2019
5.199 Impact Factor

Efficacy of pulsed electromagnetic fields and electromagnetic fields tuned to the ion cyclotron resonance frequency of Ca on chondrogenic differentiation.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Microsystems Laboratory, Institute of Microengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Previous studies provide strong evidence for the therapeutic effect of electromagnetic fields (EMFs) on different tissues including cartilage. Diverse exposure parameters applied in scientific reports and the unknown interacting mechanism of EMF with biological systems make EMF studies challenging. In 1985, Liboff proposed that when magnetic fields are tuned to the cyclotron resonance frequencies of critical ions, the motion of ions through cell membranes is enhanced, and thus biological effects appear. Read More

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http://dx.doi.org/10.1002/term.2829DOI Listing
February 2019
1 Read

The effects of dynamic compressive loading on human mesenchymal stem cell osteogenesis in the stiff layer of a bilayer hydrogel.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Chemical and Biological Engineering, University of Colorado, Boulder, CO.

Bilayer hydrogels with a soft cartilage-like layer and a stiff bone-like layer embedded with human mesenchymal stem cells (hMSCs) are promising for osteochondral tissue engineering. The goals of this work were to evaluate the effects of dynamic compressive loading (2.5% applied strain, 1 Hz) on osteogenesis in the stiff layer and spatially map local mechanical responses (strain, stress, hydrostatic pressure and fluid velocity). Read More

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http://dx.doi.org/10.1002/term.2827DOI Listing
February 2019
1 Read

Platelet-rich plasma, a powerful tool in dermatology.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Cellular and Functional Biology and Biomolecules Engineering Group, School of Science, Universidad Antonio Nariño, Bogotá, Colombia.

Platelet-rich plasma (PRP), a platelet concentrate contained in a small volume of plasma, has become a promising option in the last decade to treat different diseases related to the skin due to its high concentration of growth factors. When it is of autologous origin, it decreases the probability of suffering adverse reactions and transfusion-transmitted infections, thus it is an optimal and safe therapy for the patient. PRP has been used in the treatment of several dermatological conditions such as acne, alopecia, and skin ulcers. Read More

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http://dx.doi.org/10.1002/term.2832DOI Listing
February 2019
6 Reads

Periosteum-derived mesenchymal progenitor cells in engineered implants promote fracture healing in a critical-size defect rat model.

J Tissue Eng Regen Med 2019 Feb 20. Epub 2019 Feb 20.

Orthopaedic Surgery and Traumatology Department, Clínica Universidad de Navarra, Pamplona, Spain.

An attractive alternative to bone autografts is the use of autologous mesenchymal progenitor cells (MSCs) in combination with biomaterials. We compared the therapeutic potential of different sources of mesenchymal stem cells in combination with biomaterials in a bone nonunion model. A critical-size defect was created in Sprague-Dawley rats. Read More

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http://dx.doi.org/10.1002/term.2821DOI Listing
February 2019
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In vitro comparative study of human mesenchymal stromal cells from dermis and adipose tissue for application in skin wound healing.

J Tissue Eng Regen Med 2019 Feb 18. Epub 2019 Feb 18.

Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.

Novel strategies combining cell therapy, tissue engineering, and regenerative medicine have been developed to treat major skin wounds. Although mesenchymal stromal cells (MSCs) from different tissues have similar stem cell features, such as self-renewing mesodermal differentiation potential and expression of immunophenotypic markers, they also have distinct characteristics. Therefore, we aimed to characterize the application of MSCs derived from the dermis and adipose tissue (DSCs and ASCs, respectively) in cutaneous wound healing by in vitro approaches. Read More

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http://dx.doi.org/10.1002/term.2820DOI Listing
February 2019
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Strategies for controlling egress of therapeutic cells from hydrogel microcapsules.

J Tissue Eng Regen Med 2019 Apr 19;13(4):612-624. Epub 2019 Mar 19.

Department of Physics, University of Ottawa, Ottawa, Ontario, Canada.

Endothelial progenitor cells and human mesenchymal stem cells (hMSCs) have shown great regenerative potential to repair damaged tissue; however, their injection in vivo results in low retention and poor cell survival. Early clinical research has focussed on cell encapsulation to improve viability and integration of delivered cells. However, this strategy has been limited by the inability to reproduce large volumes of standardized microcapsules and the lack of information on cell-specific egress and timed release from hydrogel microcapsules. Read More

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http://dx.doi.org/10.1002/term.2818DOI Listing
April 2019
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Coculture with monocytes/macrophages modulates osteogenic differentiation of adipose-derived mesenchymal stromal cells on poly(lactic-co-glycolic) acid/polycaprolactone scaffolds.

J Tissue Eng Regen Med 2019 Feb 16. Epub 2019 Feb 16.

Department of Biomaterials, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, The Netherlands.

The effects of immune cells, in particular macrophages, on the behaviour of mesenchymal stromal cells (MSCs) have recently gained much attention for MSCs-based tissue-engineered constructs. This study aimed to evaluate the effect of monocytes/macrophages on the osteogenic differentiation of adipose-derived mesenchymal stromal cells (ADMSCs) in three-dimensional (3D) cocultures. For this, we cocultured THP-1 monocytes, M1 macrophages, or M2 macrophages with ADMSCs on 3D poly(lactic-co-glycolic) acid (PLGA)/polycaprolactone (PCL) scaffolds using osteogenic medium for up to 42 days. Read More

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http://doi.wiley.com/10.1002/term.2826
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http://dx.doi.org/10.1002/term.2826DOI Listing
February 2019
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Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.

J Tissue Eng Regen Med 2019 Apr 20;13(4):637-648. Epub 2019 Mar 20.

Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.

Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents. A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue-engineered products. The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking. Read More

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http://dx.doi.org/10.1002/term.2824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461514PMC
April 2019
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Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, China.

Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin-1 (Ang-1) dual gene coexpression vector that encoded green fluorescent protein (GFP) was constructed from an arginine-glycine-aspartic acid-modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze-drying method. Read More

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http://dx.doi.org/10.1002/term.2819DOI Listing
February 2019
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Low-intensity pulsed ultrasound combination with induced pluripotent stem cells-derived neural crest stem cells and growth differentiation factor 5 promotes sciatic nerve regeneration and functional recovery.

J Tissue Eng Regen Med 2019 Apr 19;13(4):625-636. Epub 2019 Mar 19.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, China.

The treatment of lengthy peripheral nerve defect is challenging in the field of nerve regeneration. Our previous studies have shown that low-intensity pulsed ultrasound (LIPUS) could promote the proliferation, cell viability, and neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) and improve the regeneration of damaged peripheral nerve. In this study, the mechanical signal transduction pathway of LIPUS promoting iPSCs-NCSCs proliferation and differentiation was further explored, and the effects of LIPUS combined with iPSCs-NCSCs, perfluorotributylamine (PFTBA), and growth differentiation factor 5 (GDF5) on the repair of peripheral nerve injury were evaluated. Read More

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http://dx.doi.org/10.1002/term.2823DOI Listing
April 2019
2 Reads
5.199 Impact Factor

Impact of modified gelatin on valvular microtissues.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Department of Human Structure and Repair, Tissue Engineering Group, Ghent University, Ghent, Belgium.

A significant challenge in the field of tissue engineering is the biofabrication of three-dimensional (3D) functional tissues with direct applications in organ-on-a-chip systems and future organ engineering. Multicellular valvular microtissues can be used as building blocks for the formation of larger scale valvular macrotissues. Yet, for the controlled biofabrication of 3D macrotissues with predefined complex shapes, directed assembly of microtissues through bioprinting is needed. Read More

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http://dx.doi.org/10.1002/term.2825DOI Listing
February 2019
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Strategic design of peptide-decorated aligned nanofibers impregnated with triiodothyronine for neural regeneration.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Biological Materials Laboratory, CSIR - Central Leather Research Institute, Chennai, India.

Nerve injuries are often debilitating as its regeneration occurs in a slow and laborious manner. Remediation of nerve injury is a colossal task as functional restoration in larger gaps seldom occurs due to the complex nerve regeneration mechanism. A nanofiber-based graft material has been fabricated to provide topographical and biochemical cues to encourage neural differentiation. Read More

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http://dx.doi.org/10.1002/term.2822DOI Listing
February 2019

Serum albumin-coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats.

J Tissue Eng Regen Med 2019 Mar 18;13(3):416-422. Epub 2019 Feb 18.

Institute of Clinical Experimental Research, Semmelweis University, Budapest, Hungary.

Serum albumin-coated bone allografts (BoneAlbumin) have successfully supported bone regeneration in various experimental models by activating endogenous progenitors. However, the effect of tissue aging, linked to declining stem cell function, has yet to be explicitly examined within the context of BoneAlbumin's regenerative capacity. Stem cell function was tested with an in vitro attachment assay, which showed that albumin coating increases stem cell attachment on demineralized bone surfaces in an aging cell population. Read More

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http://dx.doi.org/10.1002/term.2803DOI Listing