890 results match your criteria Journal of the Society for Gynecologic Investigation[Journal]


Changes in matrix metalloproteinase 2 activities in amniochorions during premature rupture of membranes.

J Soc Gynecol Investig 2006 Dec;13(8):592-7

Departments of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo, Japan.

Objective: Increased proteolytic activities of matrix metalloproteinases (MMPs) such as MMP-3 and MMP-9 are associated with premature rupture of membranes at term. However, it is unclear whether MMP-2 is involved in the premature rupture of membranes. In this study, to elucidate the role of MMP-2, we evaluated the activity of MMP-2 and also the expression of pro-MMP-2, membrane type 1 (MT1)-MMP and tissue inhibitor of metalloproteinase (TIMP)-1 in premature rupture of membranes. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.10.001DOI Listing
December 2006
8 Reads

An animal model of intrauterine growth retardation induced by synthetic thromboxane a(2).

J Soc Gynecol Investig 2006 Dec 15;13(8):566-72. Epub 2006 Nov 15.

Maternity and Perinatal Care Center, Nagoya University Hospital, Nagoya, Japan.

Objective: Intrauterine growth retardation (IUGR) is an important cause of prenatal and neonatal morbidity, and neurologic abnormalities. Although several animal models of IUGR have been developed for scientific investigation, few models approximate the pathophysiology in human fetal growth failure resulting from pregnancy-induced hypertension and preeclampsia. We developed an animal model of IUGR in which fetal growth restriction was induced by administering a synthetic thromboxane A(2) analogue (STA(2)) to the mother. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.09.007
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http://dx.doi.org/10.1016/j.jsgi.2006.09.007DOI Listing
December 2006
6 Reads

Leptin suppresses human chorionic gonadotropin-induced cyclooxygenase-2 expression and prostaglandin production in cultured human granulose luteal cells.

J Soc Gynecol Investig 2006 Dec 9;13(8):551-7. Epub 2006 Nov 9.

Department of Obstetrics and Gynecology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.

Objective: In a previous study, we demonstrated that high leptin levels at the time of human chorionic gonadotropin (hCG) injection impaired the pregnancy rate for women undergoing in vitro fertilization. In this study we examine leptin's effect on prostaglandin formation and cyclooxygenase (COX) expression induced by hCG in human granulose luteal (GL) cells.

Methods: Human GL cells were obtained from women undergoing ovarian hyperstimulation. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.09.004DOI Listing
December 2006
7 Reads

Chronic tumor necrosis factor-alpha infusion in gravid C57BL6/J mice accelerates adipose tissue development in female offspring.

J Soc Gynecol Investig 2006 Dec 7;13(8):558-65. Epub 2006 Nov 7.

Department of Obstetrics and Gynecology, Katholieke Universiteit Leuven, Leuven, Belgium.

Objective: Tumor necrosis factor (TNF)-alpha is thought to mediate, in part, the link between obesity and insulin resistance, and women with gestational diabetes mellitus (GDM) have raised serum TNF-alpha concentrations. Our objective was to investigate whether systemic TNF-alpha administration into gravid C57BL6/J mice causes a GDM-like syndrome and affects growth and adipose tissue (AT) development in the offspring.

Methods: We assessed glucose tolerance and reproductive outcome in mice infused with saline, or 2 mug or 4 mug recombinant mouse (rm)TNF-alpha by subcutaneous mini-osmotic pumps between days (d)11. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.09.001DOI Listing
December 2006
8 Reads

Genetic associations in preterm birth: a primer of marker selection, study design, and data analysis.

J Soc Gynecol Investig 2006 Dec 7;13(8):531-41. Epub 2006 Nov 7.

The Perinatal Research Center, Nashville, TN 37203, USA.

Spontaneous preterm birth (PTB; delivery before 37 weeks gestation) is a primary risk factor for infant morbidity and mortality. The etiology is unclear, but there is evidence that there is a genetic predisposition to PTB. Armed with the suggestion of genetic risk factors and the failure to identify useful biomarkers, investigators are starting to actively pursue the role of genetic predisposition in PTB. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.09.006DOI Listing
December 2006
7 Reads

Estrogen metabolite 2-methoxyestradiol induces apoptosis and inhibits cell proliferation and collagen production in rat and human leiomyoma cells: a potential medicinal treatment for uterine fibroids.

J Soc Gynecol Investig 2006 Dec 7;13(8):542-50. Epub 2006 Nov 7.

Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston, Texas.

Objective: The current study sought to investigate the effect of the estrogen metabolite 2-methoxyestradiol (2-MeOHE(2)) on apoptosis, cell proliferation, and collagen synthesis in human and rat leiomyoma cells.

Methods: [(3)H] thymidine and [(3)H] proline incorporation studies were conducted. The expression of vascular endothelial growth factor (VEGF), cyclin D1, Bcl-2, and Bax were evaluated by Western blot. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.09.003
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http://dx.doi.org/10.1016/j.jsgi.2006.09.003DOI Listing
December 2006
5 Reads

Non-muscle myosin-II-B filament regulation of paracellular resistance in cervical epithelial cells is associated with modulation of the cortical acto-myosin.

J Soc Gynecol Investig 2006 Dec 7;13(8):579-91. Epub 2006 Nov 7.

Departments of Reproductive Biology, Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, USA.

Objective: To understand myosin regulation of epithelial permeability.

Methods: This was an experimental study, using human cervical epithelial cells CaSki. End points were paracellular permeability (determined in terms of transepithelial electrical resistance); non-muscle myosin-II-B (NMM-II-B) cellular localization; NMM-II-B phosphorylation status; NMM-II-B-actin interaction (determined in vitro by the immunoprecipitation-immunoreactivity method); and NMM-II-B filamentation (determined in vitro using purified NMM-II-B filaments in terms of filaments disassembly/assembly ratios. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.09.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1850386PMC
December 2006
8 Reads

Combined thyroidectomy and renal denervation suppress renin expression and secretion in fetal sheep.

J Soc Gynecol Investig 2006 Dec 23;13(8):604-9. Epub 2006 Oct 23.

Department of Obstetrics/Gynecology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157-1066, USA.

Background And Objectives: Activity of the fetal renin-angiotensin system (RAS) is developmentally regulated, increasing in late gestation toward term. Thyroid hormone and the renal nerves are both important modulators of renal RAS maturation; however, ablation of either influence alone does not totally block the aforementioned developmental late gestation increase in RAS in fetal sheep. In the current study, we used the technique of thyroidectomy combined with bilateral renal denervation (TX+D), which removes thyroid hormone from the circulation and abolishes effects of renal nerve activity, to determine if simultaneous removal of their effects on the kidney would markedly alter renin expression and secretion in late gestation. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.07.006
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http://dx.doi.org/10.1016/j.jsgi.2006.07.006DOI Listing
December 2006
4 Reads

A low plasma volume in formerly preeclamptic women predisposes to the recurrence of hypertensive complications in the next pregnancy.

J Soc Gynecol Investig 2006 Dec 23;13(8):598-603. Epub 2006 Oct 23.

Departments of Obstetrics and Gynecology, University Hospital Maastricht, Maastricht, The Netherlands.

Background: Formerly preeclamptic women with a subnormal plasma volume (PV) have an increased risk to develop a hypertensive disorder in a subsequent pregnancy as compared to women with normal PV. In the current study we tested the hypothesis that formerly preeclamptic women who develop recurrent disease in their next pregnancy differ from their counterparts with an uneventful next pregnancy by a lower pre-pregnant PV, a lower venous capacitance, smaller rises in these indices in early pregnancy, a lower renal adaptive response, and a lower response to mild exercise.

Patients And Methods: We enrolled 33 formerly preeclamptic women in this study. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.07.008DOI Listing
December 2006
6 Reads

Alterations in the maternal peripheral microvascular response in pregnancies complicated by preeclampsia and the impact of fetal sex.

J Soc Gynecol Investig 2006 Dec 19;13(8):573-8. Epub 2006 Oct 19.

Mother and Babies Research Centre, Hunter Medical Research Institute, University of Newcastle, Newcastle, NSW, Australia.

Objective: Peripheral microvascular function is altered in preeclampsia (PE). Recent studies suggest that maternal physiology varies with fetal sex. We wanted to examine if there were sex-specific differences in maternal peripheral microvascular function in normal pregnancy and pregnancy complicated by PE. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.06.006
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http://dx.doi.org/10.1016/j.jsgi.2006.06.006DOI Listing
December 2006
6 Reads

Tissue prothrombinase activity in myometrium from timed-pregnant rats.

J Soc Gynecol Investig 2006 Oct;13(7):477-82

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.

Objective: Fgl2 and thrombin potentially play roles during inflammation-induced preterm delivery. These studies sought to demonstrate functional prothrombinase enzyme activity in rat uterus, consistent with the expression of Fgl2 in this tissue.

Methods: Myometrial and other tissue obtained from non-pregnant and timed-pregnant rats was homogenized in Tris-buffered saline. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.07.009DOI Listing
October 2006
3 Reads

Matrix metalloproteinase-1 and -9 promoter polymorphisms and endometrial carcinoma risk in a Japanese population.

J Soc Gynecol Investig 2006 Oct 20;13(7):523-9. Epub 2006 Sep 20.

Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan.

Objective: The matrix metalloproteinases (MMPs) are a family of zinc-dependent proteases that degrade all the components of the extracellular matrix (ECM). Several studies have demonstrated association between MMP gene polymorphisms and various cancers. The object of this study was to investigate whether the MMP-1 and MMP-9 gene promoter polymorphisms are associated with endometrial carcinomas in a Japanese population. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.07.001
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http://dx.doi.org/10.1016/j.jsgi.2006.07.001DOI Listing
October 2006
6 Reads

Estrogen receptor-beta is the predominant estrogen receptor subtype in normal human synovia.

J Soc Gynecol Investig 2006 Oct 20;13(7):512-7. Epub 2006 Sep 20.

Department of Obstetrics and Gynecology, Division of Gynecologic Endocrinology and Reproductive Medicine, Medical University of Vienna, Vienna, Austria.

Objective: Joint pain increases after menopause with more than 50% of woman suffering from arthralgies. Since pain and inflammation of joints originate from synovial tissue, we aimed to discover whether estrogen receptors are present in the human synovia.

Methods: This in vitro study was performed on samples of human synovial tissue, obtained from pre- (n = 8) and postmenopausal woman (n = 11) and men (n = 5) following surgery due to traumatic lesions. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.07.002DOI Listing
October 2006
10 Reads

Oxidant/antioxidant status of placenta, blood, and cord blood samples from pregnant women supplemented with iron.

J Soc Gynecol Investig 2006 Oct 20;13(7):502-5. Epub 2006 Sep 20.

Ankara University School of Medicine, Department of Biochemistry, Ankara, Turkey.

Objective: In this study, it was aimed to investigate oxidant/antioxidant status in placenta and in blood and cord blood samples from pregnant women supplemented with iron during pregnancy.

Methods: For this purpose, 27 pregnant women at admission for delivery participated in the study. Fifteen of them did not take iron tablets and the others took oral iron supplements during pregnancy. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.07.004DOI Listing
October 2006
18 Reads

The role of endometrium in endometriosis.

J Soc Gynecol Investig 2006 Oct 20;13(7):467-76. Epub 2006 Sep 20.

Yale University School of Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, New Haven, Connecticut 06520-8063, USA.

Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus. Several theories have been proposed to explain the pathogenesis of this disease. According to Sampson's retrograde menstruation theory, endometrial cells are refluxed through the fallopian tubes during the menstruation and implant onto peritoneum or pelvic organs. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.07.005DOI Listing
October 2006
8 Reads

The pattern of glucocorticoid and estrogen receptors may explain differences in steroid dependency of intrauterine prostaglandin production at parturition in sheep.

J Soc Gynecol Investig 2006 Oct 20;13(7):506-11. Epub 2006 Sep 20.

Canadian Institutes of Health Research Group in Fetal and Neonatal Health and Development, Canada.

Background: We have recently described two distinct pathways of intrauterine prostaglandin (PG) synthesis: a cortisol-dependent/estradiol-independent mechanism within trophoblast tissue leading to elevations in fetal plasma PGE2, and an estradiol-dependent mechanism within maternal endometrium that leads to increased maternal plasma PGF2(2alpha). We hypothesized that the differential effects of cortisol and estradiol on intrauterine PGH synthase-II (PGHS-II) expression and PG production may be because of the tissue specific expression of the glucocorticoid and estradiol receptors (GR and ER, respectively) within the intrauterine tissues. In addition, we suggest that these two pathways of PG production are linked through the expression of P450(C17hydroxylase) (P450(C17)) and subsequent increase in placental estradiol synthesis. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.07.003DOI Listing
October 2006
7 Reads

Lack of site-specific production of decidual alpha-2 macroglobulin in human pregnancy.

J Soc Gynecol Investig 2006 Oct 15;13(7):491-6. Epub 2006 Sep 15.

Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.

Background: Alpha-2 Macroglobulin (A2M) is a protease inhibitor that is present in both human and rat decidual tissue. In mice, decidual A2M prevents excessive trophoblastic invasion; however, its role in human decidual tissue is unknown. It is possible that A2M may also influence trophoblast invasion in human pregnancy, which would be reflected in increased A2M production in decidua basalis. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.06.003DOI Listing
October 2006
6 Reads

Phospholipid scramblase isoform expression in pregnant rat uterus.

J Soc Gynecol Investig 2006 Oct 15;13(7):497-501. Epub 2006 Sep 15.

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, Vermont 05403, USA.

Objective: Phospholipid scramblases (PLSCRs), a family of novel membrane proteins, facilitate the translocation of aminophospholipids from the inner to the exterior leaf of the cell membrane. Four isoforms of PLSCR (PLSCR1-4) have been reported in mouse and human. The studies described in this report sought to characterize the uterine expression of the PLSCR isoforms in the near-term pregnant rat. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.06.002DOI Listing
October 2006
3 Reads

Potential use of the adenosine triphosphate cell viability assay in endometrial cancer.

J Soc Gynecol Investig 2006 Oct 15;13(7):518-22. Epub 2006 Sep 15.

Gynaecological Oncology Division, Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, China.

Objective: Adenosine triphosphate cell viability assay (ATP-CVA) was used previously to evaluate chemotherapy in uterine cancer cell lines. In this study, we have performed the ATP-CVA on endometrial cancer patients to study the feasibility of using ATP-CVA in endometrial cancer to determine the intrinsic chemosensitivity of the cytotoxic drugs.

Methods: Thirty-three patients with endometrial adenocarcinoma who presented for a staging operation were recruited. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.06.004DOI Listing
October 2006
31 Reads

Differential expression of endothelial nitric oxide synthase in coronary and cardiac tissue in hypoxic fetal guinea pig hearts.

J Soc Gynecol Investig 2006 Oct 18;13(7):483-90. Epub 2006 Sep 18.

Department of Obstetrics, Gynecology and Reproductive Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.

Objective: The purpose of the present study was to quantify the effect of chronic hypoxia on endothelial nitric oxide synthase (eNOS) gene and protein expression of fetal coronary artery segments and cardiac tissue of fetal guinea pig hearts.

Methods: Time-mated pregnant guinea pigs (term = 65 days) were housed in room air (NMX, n = 6) or in a hypoxic chamber containing 10.5% O2 for 14 days (HPX14, n = 6). Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.06.005DOI Listing
October 2006
4 Reads

Systemic activation and vascular infiltration of neutrophils with term labor.

J Soc Gynecol Investig 2006 Sep;13(6):425-9

Departments of Obstetrics and Gynecology and Physiology, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298-0034, USA.

Objective: The purpose of the current study was to determine if neutrophils infiltrate maternal systemic vascular tissue at the time of term labor.

Methods: Subcutaneous fat biopsies were obtained at cesarean delivery or abdominal surgery from laboring women (n = 5), non-laboring women (n = 5), and normal non-pregnant women (n = 5). Immunohistochemical staining was performed for CD66b, a neutrophil antigen, and intercellular adhesion molecule-1 (ICAM-1; CD54), an endothelial cell adhesion molecule for neutrophils. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.06.001DOI Listing
September 2006
5 Reads

Effect of betamethasone in vivo on placental adrenomedullin in human pregnancy.

J Soc Gynecol Investig 2006 Sep 4;13(6):418-24. Epub 2006 Aug 4.

Department of Gynecology, Perinatology and Child Health, University La Sapienza, Rome, Italy.

Objective: The aim of the current study was to determine the effects of in vivo administration of prenatal betamethasone in patients at risk for preterm delivery on adrenomedullin (AM) concentrations in maternal and fetal plasma and on AM localization in placenta and fetal membranes.

Methods: A total of 62 pregnant women between 25 and 35 weeks' gestation were studied. Forty-seven pregnant women received betamethasone (2 x 12 mg intramuscularly given 24 hours apart) for stimulation of fetal lung maturity. Read More

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http://rsx.sagepub.com/cgi/doi/10.1016/j.jsgi.2006.05.003
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http://dx.doi.org/10.1016/j.jsgi.2006.05.003DOI Listing
September 2006
5 Reads

Up-regulation of the endothelin receptor A in placental tissue from first trimester delayed miscarriages.

J Soc Gynecol Investig 2006 Sep 31;13(6):435-41. Epub 2006 Jul 31.

Department of Obstetrics and Gynaecology, Medical University of Graz, Graz, Austria.

Objective: This study tested the hypothesis that the endothelin (ET)/ET receptor (ETR) system in biologic fluids and in the human placenta is altered in delayed miscarriages as compared to apparently normal early pregnancies (reference group).

Methods: Immunoreactive ET (irET) concentrations were measured in plasma, urine, and cervical smears from 57 pregnant women in the weeks 6 to 14 of gestation (46 delayed miscarriages, 11 references) with radioimmunoassay (RIA). ET-1, ETR-A, and ETR-B mRNA, and ETR protein expression were measured in placental tissue of 45 early pregnancies (31 delayed miscarriages, 14 references) using semiquantitative reverse-transcription polymerase chain reaction (RT-PCR) and immunoblotting, respectively. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.05.006
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http://dx.doi.org/10.1016/j.jsgi.2006.05.006DOI Listing
September 2006
7 Reads

Reduced cellular glutathione reductase activity and increased adhesion molecule expression in endothelial cells cultured with maternal plasma from women with preeclampsia.

J Soc Gynecol Investig 2006 Sep 31;13(6):412-7. Epub 2006 Jul 31.

Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA.

Objective: The purpose of the current study was to determine whether maternal circulating components could regulate oxidative status of glutathione redox cycle and adhesion molecule expression in endothelial cells (ECs).

Methods: Maternal plasma was extracted from venous blood obtained from normal term pregnant women and from women with preeclampsia (PE). Normal and PE pregnancies were defined as American College of Obstetricians and Gynecologists criteria. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.05.009
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http://dx.doi.org/10.1016/j.jsgi.2006.05.009DOI Listing
September 2006
7 Reads

Hyperpolarization-activated cation channels are expressed in rat hypothalamic gonadotropin-releasing hormone (GnRH) neurons and immortalized GnRH neurons.

J Soc Gynecol Investig 2006 Sep 31;13(6):442-50. Epub 2006 Jul 31.

Department of Gynecology-Obstetrics, University at Buffalo, School of Medicine and Biomedical Sciences, Buffalo, New York 14222, USA.

Objectives: The current research was conducted to determine whether hyperpolarization-activated cyclic nucleotide-gated (HCN1-4) channels are expressed in gonadotropin-releasing hormone (GnRH) neurons in the female rat hypothalamus and immortalized GnRH neurons (GT1-7 cells).

Methods: Double-label fluorescence immunohistochemistry was used to colocalize HCN1-4 channels and GnRH in GnRH neurons in the female rat hypothalamus. Reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and immunocytochemistry were used to analyze HCN channel gene expression in GT1-7 cells. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.010DOI Listing
September 2006
4 Reads

Redox-induced apoptosis of human oocytes in resting follicles in vitro.

J Soc Gynecol Investig 2006 Sep 31;13(6):451-8. Epub 2006 Jul 31.

Peking University First Hospital, Institute of Urology of Peking University, Beijing, P.R. China.

Background: The age-related depletion of the resting follicle (RF) stock occurs as a result of two processes: atresia and entry in growth phase. Due to difficulties in obtaining sufficient numbers of RFs for study, little is known about the apoptotic mechanisms for RF atresia. The present study was designed to investigate the effects of oxidative stress on the apoptosis of RF oocytes. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.005DOI Listing
September 2006
8 Reads

Endothelial activation and cell adhesion molecule concentrations in pregnant women living at high altitude.

J Soc Gynecol Investig 2006 Sep 31;13(6):399-403. Epub 2006 Jul 31.

Maternal and Fetal Medicine Section, Institute of Medical Genetics, Yorkhill, Glasgow, UK.

Objectives: Maternal physiology at high altitude could be considered to resemble an intermediate state between preeclampsia and normal pregnancy. The objective of the current study was to determine if cell adhesion molecules, known to be increased in preeclampsia, are increased with chronic maternal and placental hypoxia (due to high-altitude residence) in the absence of preeclampsia.

Methods: Serum was collected from women residing at 3100 m or 1600 m in the three trimesters of pregnancy and postpartum. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.001DOI Listing
September 2006
4 Reads

Prolonged mild hypoxia alters fetal sheep electrocorticogram activity.

J Soc Gynecol Investig 2006 Sep 31;13(6):404-11. Epub 2006 Jul 31.

Perinatal Research Laboratory, Department of Obstetrics and Gynecology, Center for Research in Obstetrics and Gynecology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.

Objective: To assess the effects of prolonged mild hypoxemia on fetal brain electrocorticogram (ECoG) in late gestation.

Study Design: Fetal and maternal catheters were placed under general anesthesia and animals allocated at random to receive intratracheal maternal administration of either nitrogen (n = 8) or compressed air (n = 8). Five days after surgery (125 days' gestational age), nitrogen infusion was adjusted to reduce fetal brachial artery PO2 by 25%. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.007DOI Listing
September 2006
11 Reads

Progestagenic effects of tibolone are target gene-specific in human endometrial cells.

J Soc Gynecol Investig 2006 Sep 31;13(6):459-65. Epub 2006 Jul 31.

Department of Obstetrics/Gynecology and Reproductive Medicine, SUNY-Stony Brook, Stony Brook, New York 11794, USA.

Objectives: Tibolone (Tib) exhibits progestagenic activities in addition to its tissue-specific estrogenic activities. The purpose of the current study was to determine the progestagenic actions of Tib and its metabolites using target genes known to be regulated by progestins in human endometrial glandular and stromal cells.

Methods: Human endometrial glandular and stromal cells were isolated from endometrial tissue fragments and separately incubated with Tib and its metabolites. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.004DOI Listing
September 2006
4 Reads

Vaginal matrix metalloproteinase levels in pregnant women with bacterial vaginosis.

J Soc Gynecol Investig 2006 Sep 26;13(6):430-4. Epub 2006 Jul 26.

Research Unit in Reproductive Medicine, Teaching and Research Program, Maternal-Fetal Medicine Department, UMAE en Ginecologia y Obstetricia Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, Mexico D F, Mexico.

Objective: To compare matrix metalloproteinase (MMP)-8 and MMP-9 levels in the vaginal secretions of pregnant women with or without asymptomatic bacterial vaginosis (BV).

Methods: In this study, vaginal levels and molecular forms of MMP-8 and MMP-9 were studied in 36 pregnant women between 28 and 34 weeks of gestation with asymptomatic BV and 41 pregnant women, matched for gestational age, without BV.

Results: Vaginal MMP-8 concentrations were significantly higher (P = . Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.008DOI Listing
September 2006
5 Reads

Female infertility and free radicals: potential role in adhesions and endometriosis.

J Soc Gynecol Investig 2006 Sep 26;13(6):390-8. Epub 2006 Jul 26.

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, C.S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan 48201, USA.

Free radicals are highly reactive molecules produced in the cell either as part of, or as end-products of, biochemical reactions that have crucial roles in the homeostasis of the organism. Thus, excess production or impaired elimination of free radicals leads to increased oxidative stress, which has been implicated in the development of several different disease states, including hypoxia-reperfusion injury, cancer, and aging. Peritoneal adhesions and endometriosis are relatively commonly identified in women, and are known to be associated with infertility without clearly understood pathophysiology. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.05.002DOI Listing
September 2006
9 Reads

Investigation of free radical scavenging enzyme activities and lipid peroxidation in human placental tissues with miscarriage.

J Soc Gynecol Investig 2006 Jul;13(5):384-8

Background: Miscarriage (early pregnancy failure) is a pregnancy-related disease, the pathophysiology of which is still not completely understood. Lipid peroxidation and alterations in antioxidant enzyme activities may be of importance in the pathogenesis of this disorder. This study was planned to investigate the possible relation between free radical scavenging enzyme activities and lipid peroxidation levels in placenta tissues with miscarriage. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.04.003DOI Listing
July 2006
12 Reads

Decreased plasma visfatin concentrations in women with gestational diabetes mellitus.

J Soc Gynecol Investig 2006 Jul;13(5):364-7

Department of Obstetrics and Gynecology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.

Objective: To test the hypothesis that plasma visfatin concentrations will be lower in women with gestational diabetes mellitus, we evaluated women with gestational diabetes mellitus and healthy pregnant women, and then correlated their plasma visfatin concentrations with body mass index (BMI) and various other parameters.

Methods: A total of 40 women were evaluated: 20 women with gestational diabetes mellitus and 20 healthy pregnant women to serve as control subjects. Plasma visfatin concentrations were analyzed using an enzyme-linked immunosorbent assay. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.04.007DOI Listing
July 2006
6 Reads

Placental lactate transporter activity and expression in intrauterine growth restriction.

J Soc Gynecol Investig 2006 Jul;13(5):357-63

Division of Human Development, University of Manchester, St. Mary's Hospital, Manchester, United Kingdom.

Objectives: To compare lactate uptake in the microvillous plasma membrane (maternal facing [MVM]) in term and preterm placentas in intrauterine growth restriction (IUGR) and appropriate weight for gestational age (AGA) controls, and in the basal plasma membrane (fetal facing [BM]) at term. In addition, we examine the expression of monocarboxylate transporters (MCT1 and MCT4).

Methods: We measured [14C] L-lactate uptakes into vesicles prepared from MVM and BM, stimulated by an inwardly directed H+ gradient. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.04.006DOI Listing
July 2006
3 Reads

Multiplex measurement of cytokine/receptor gene polymorphisms and interaction between interleukin-10 (-1082) genotype and chorioamnionitis in extreme preterm delivery.

J Soc Gynecol Investig 2006 Jul;13(5):350-6

Department of Gynecology and Obstetrics, Hannover Medical School, Hannover, Germany.

Objectives: To establish a multiplex amplification refractory mutation system (ARMS) in fluid and dried whole blood, and to perform a pilot study to examine the role for single-nucleotide polymorphisms (SNPs) of inflammation-associated genes (interleukin [IL]-1 and -10, tumor necrosis factor-alpha [TNFA], and toll-like receptor-4 [TLR4]) and their interaction with clinical chorioamnionitis (CAM) in prematurity.

Methods: We established a quadruplex ARMS to detect the four above SNPs. Fifty-four women delivered at gestational age less than 32 weeks and 83 healthy female volunteers were genotyped. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.04.004
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http://dx.doi.org/10.1016/j.jsgi.2006.04.004DOI Listing
July 2006
8 Reads

Mechanisms of paracrine regulation by fetal membranes of human uterine quiescence.

J Soc Gynecol Investig 2006 Jul;13(5):343-9

Departmento de Obstetricia y Ginecología, Unidad de Medicina Materno Fetal, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.

Objective: To test the hypothesis that fetal membranes (chorion or amnion) release one or more factors responsible for myometrial quiescence.

Methods: Myometrial samples were excised from women at elective term cesarean delivery prior to the onset of labor. Fetal membranes were obtained after cesarean delivery either before or during labor, and either term (greater than 37 weeks) or preterm (less than or equal to 36 weeks). Read More

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http://rsx.sagepub.com/cgi/doi/10.1016/j.jsgi.2006.04.005
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http://dx.doi.org/10.1016/j.jsgi.2006.04.005DOI Listing
July 2006
7 Reads

SHP protein tyrosine phosphatase expression in rat uterine tissue.

J Soc Gynecol Investig 2006 Jul;13(5):338-42

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.

Objective: Enhanced tyrosine phosphorylation of phospholipase C-gamma1 (PLCgamma1) is associated with increased spontaneous contractile activity. PLCgamma1 phosphorylation is regulated by cellular protein tyrosine kinases and tyrosine phosphatases (PTPs). The studies in this report were undertaken to characterize the expression of two PTPs known to bind to PLCgamma1: Src-homology phosphatase type-1 (SHP-1) and type-2 (SHP-2). Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.04.008DOI Listing
July 2006
3 Reads

Intracytoplasmic sperm injection--an assisted reproduction technique that should make us cautious about imprinting deregulation.

J Soc Gynecol Investig 2006 Jul;13(5):317-28

Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Dragana, Alexandroupolis, Greece.

Objective: Due to the extensive use of intracytoplasmic sperm injection (ICSI) in assisted reproduction, not only among couples with severe male factor infertility problems, but to a broader scale, a lot of concern has been raised regarding the safety of the method and its implications in epigenetic control and imprinting dysregulation. This review means to provide a comprehensive report of the published scientific data, outline putative associations between ICSI and epigenetic control, and suggest measures to improve the current state of affairs and reach more scientifically consolidated results.

Methods: This review was conducted by studying a broad spectrum of articles dealing with the subject of epigenetic control and its relation with ICSI. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.04.002
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http://dx.doi.org/10.1016/j.jsgi.2006.04.002DOI Listing
July 2006
8 Reads

Expression of nitric oxide synthase isoforms in the ovine fetal brain: alteration by hormonal and hemodynamic stimuli.

J Soc Gynecol Investig 2006 Jul 6;13(5):329-37. Epub 2006 Jun 6.

Department of Physiology and Functional Genomics, University of Florida College of Medicine, Gainesville, Florida 32610-0274, USA.

Objective: Nitric oxide (NO) is synthesized in the brain through the action of three isoforms of nitric oxide synthase (NOS). The local generation of NO in neurons, glia, and vasculature modulates neuronal activity, as well as regional cerebral blood flow. We propose that, in the fetal brain, cerebral hypoperfusion alters the expression of NOS isoforms, and that estrogen administration modulates the NOS response to hypoperfusion. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.04.001
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http://dx.doi.org/10.1016/j.jsgi.2006.04.001DOI Listing
July 2006
8 Reads

Association of two polymorphisms in the peroxisome proliferator-activated receptor-gamma gene with adenomyosis, endometriosis, and leiomyomata in Japanese women.

J Soc Gynecol Investig 2006 Jul 24;13(5):372-7. Epub 2006 May 24.

Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan.

Objective: The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is a nuclear hormone receptor that plays an important role in many diseases. This study investigated whether two polymorphisms (Pro12Ala in exon B and C161T in exon 6) of the PPAR-gamma2 gene are related to adenomyosis, endometriosis, or leiomyomata.

Methods: A total of 390 patients with adenomyosis, endometriosis, and/or leiomyomata were classified into four groups: 103 patients with adenomyosis (21 adenomyosis only and 82 adenomyosis with endometriosis and/or leiomyomata), 95 patients with endometriosis only, 100 patients with leiomyomata only, and 92 patients with endometriosis and leiomyomata. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.03.005DOI Listing
July 2006
6 Reads

Insulin-like growth factor-I regulates vascular endothelial growth factor secretion by human oviductal epithelial cells and stromal fibroblasts.

J Soc Gynecol Investig 2006 Jul 19;13(5):368-71. Epub 2006 May 19.

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Yufu-shi, Oita, Japan.

Objective: The aim of the current study is to evaluate the effect of insulin-like growth factor-I (IGF-I) on the production of vascular endothelial growth factor (VEGF) in the human fallopian tube.

Methods: Human oviductal epithelial cells (OEC) and oviductal stromal fibroblasts (OSF) were isolated from the ampullary segment of the fallopian tubes of six premenopausal patients in the proliferative phase of the menstrual cycle. The secretion of VEGF165 by cultured OEC and OSF in response to IGF-I was measured using an enzyme-linked immunosorbent assay (ELISA). Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.03.007DOI Listing
July 2006
4 Reads

Leflunomide--an immunomodulator--induces regression of endometrial explants in a rat model of endometriosis.

J Soc Gynecol Investig 2006 Jul 19;13(5):378-83. Epub 2006 May 19.

Department of Obstetrics and Gynecology, Zekai Tahir Burak Women Health Care, Research and Education Hospital, Ankara, Turkey.

Objective: To test if leflunomide, an immunomodulator, could impede the growth of an ectopic uterine tissue.

Methods: Endometriosis was surgically induced in 26 rats by transplanting an autologous fragment of endometrial tissue onto the inner surface of the abdominal wall. Four weeks later two rats were killed. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.03.006DOI Listing
July 2006
4 Reads

Potassium channels in the vasodilating action of levosimendan on the human umbilical artery.

J Soc Gynecol Investig 2006 May;13(4):312-5

Objective: Levosimendan is a calcium-sensitizing agent and inodilator working via potassium channels, which is under current investigation in the treatment of heart failure. We investigated the type of potassium channels that play a role on the dilatating effect of levosimendan on the contractile tones of the isolated human umbilical artery (HUA).

Methods: The response in the HUA was recorded isometrically by a force displacement transducer in isolated organ baths. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.02.005DOI Listing
May 2006
3 Reads

The granulin-epithelin precursor is a steroid-regulated growth factor in endometrial cancer.

J Soc Gynecol Investig 2006 May;13(4):304-11

Mayo Clinic, Gynecologic Surgery, Rochester, Minnesota 55905, USA.

Objectives: The majority of endometrial cancers arise as a result of estrogen stimulation, the molecular targets of which remain incompletely defined. We hypothesize that the granulin-epithelin precursor (GEP) may be one such target. In this study, we examined the frequency of GEP and estrogen receptor (ER) co-expression in human endometrial cancers. Read More

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http://journals.sagepub.com/doi/10.1016/j.jsgi.2006.03.003
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http://dx.doi.org/10.1016/j.jsgi.2006.03.003DOI Listing
May 2006
16 Reads

Preoperative treatment of uterine leiomyomas: clinical findings and expression of transforming growth factor-beta3 and connective tissue growth factor.

J Soc Gynecol Investig 2006 May;13(4):297-303

Dipartimento di Scienze Ostetrico-Ginecologiche, Urologiche e Medicina della Riproduzione, Universitá degli Studi di Napoli Federico II, Napoli, Italia.

Objective: To evaluate the clinical features and the expression of transforming growth factor-beta3 (TGF-beta3) and connective tissue growth factor (CTGF) in myometrium and uterine leiomyomas after preoperative treatment with gonadotropin-releasing hormone-analogs (GnRH-a) and tibolone.

Methods: Twenty-three patients received 3.75 mg leuprolide acetate depot for 4 months. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.02.008DOI Listing
May 2006
7 Reads

The depolarized expression of the alpha-6 integrin subunit in the endometria of women with endometriosis.

J Soc Gynecol Investig 2006 May;13(4):292-6

Department of Obstetrics and Gynecology, Hospital Universitari del Mar, Barcelona, Spain.

Objective: The current study sought to compare the endometrial localization of the integrin subunit alpha-6 in women with endometriosis and women without the disease. Alpha-6 integrins have an important function, not only in the attachment of cells to the extracellular matrix and laminin, but they also serve as inductors of cell migration and invasion, depending on their pattern of expression in the cell membrane.

Methods: The endometriosis group consisted of 32 women with a confirmed diagnosis of endometriosis by laparoscopy or laparotomy. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.03.002DOI Listing
May 2006
26 Reads
2 Citations

Analysis of free hemoglobin level and hemoglobin peptides from human puerperal uterine secretions.

J Soc Gynecol Investig 2006 May;13(4):285-91

Department of Analytical Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland.

Objective: Hemocidins are a novel class of antibacterial peptides generated proteolytically from hemoglobin. These peptides play a particularly important role in maintaining vaginal homeostasis during menstrual bleeding. To investigate the hemoglobin fragmentation process during the last stages of pregnancy, we examined uterine secretion (lochia) samples from a group of 22 healthy women who underwent cesarean delivery at term. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.02.009DOI Listing
May 2006
3 Reads

Association of fetal choroid plexus cysts with trisomy 18 in a population previously screened by nuchal translucency thickness measurement.

J Soc Gynecol Investig 2006 May;13(4):280-4

Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Linkou Medical Center, Taoyuan, Taiwan, ROC.

Objective: The study's aim was to determine any association between choroid plexus cysts (CPCs) and trisomy 18 in a population of fetuses previously screened by nuchal translucency (NT) thickness measurement.

Methods: During the study period (May 1999 to December 2004), 7,795 fetuses had an NT scan and second-trimester fetal anatomical scan at our institution. The prevalence of trisomy 18 was determined among four types of pregnancies: 1) those with isolated CPCs, 2) those with CPCs and enlarged NT, 3) those with CPCs and other ultrasound markers, and 4) those with CPCs, enlarged NT, and other ultrasound markers. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.02.013DOI Listing
May 2006
6 Reads

Differential secretion of matrix metalloproteinase-2 and -9 after selective infection with group B streptococci in human fetal membranes.

J Soc Gynecol Investig 2006 May;13(4):271-9

Direction of Research, Nacional de Perinatologia, Mexico City, Mexico.

Objective: This study evaluated the secretions of zymogen and active forms of matrix metalloproteinase (MMP)-9 and MMP-2 and their specific inhibitors, TIMP-1 and TIMP-2 by fetal membranes stimulated with group B Streptoccocci (GBS).

Methods: We used an in vitro experimental model that allowed us to estimate the individual contribution of the amnion (AM) and the choriodecidua (CHD) to the microbial insult. Membranes were obtained after delivery by elective cesarean delivery from women at 37 to 40 weeks of gestation without evidence of either active labor or intrauterine infection. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.02.006DOI Listing
May 2006
25 Reads

Macrophage migration inhibitory factor-nitric oxide interaction in human fetal membranes at term pregnancy.

J Soc Gynecol Investig 2006 May;13(4):263-70

Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy.

Objectives: Macrophage migration inhibitory factor (MIF), a multifunctional proinflammatory cytokine, has been recently involved in many aspects of reproduction including pregnancy. However, no evidence is available on the role of MIF in gestational tissues nor on factors regulating MIF production. This study, conducted on explants of human fetal membranes at term gestation, has been undertaken to investigate whether: (1) MIF is produced by fetal membranes; (2) nitric oxide (NO) can regulate local MIF production; and (3) MIF, in turn, can influence NO release in these tissues. Read More

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http://dx.doi.org/10.1016/j.jsgi.2006.02.010DOI Listing
May 2006
6 Reads