8,856 results match your criteria Journal of the American Society of Nephrology : JASN[Journal]


Fluconazole Increases Osmotic Water Transport in Renal Collecting Duct through Effects on Aquaporin-2 Trafficking.

J Am Soc Nephrol 2019 Apr 15. Epub 2019 Apr 15.

Max Delbrück Center for Molecular Medicine Berlin, (MDC), Research area Cardiovascular & Metabolic Disease, Berlin, Germany;

Background: Arginine-vasopressin (AVP) binding to vasopressin V2 receptors promotes redistribution of the water channel aquaporin-2 (AQP2) from intracellular vesicles into the plasma membrane of renal collecting duct principal cells. This pathway fine-tunes renal water reabsorption and urinary concentration, and its perturbation is associated with diabetes insipidus. Previously, we identified the antimycotic drug fluconazole as a potential modulator of AQP2 localization. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018060668DOI Listing

An Antifungal for Antidiuresis?

J Am Soc Nephrol 2019 Apr 12. Epub 2019 Apr 12.

Division of Endocrinology and Metabolism, Department of Medicine, Georgetown University, Washington, DC

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019030285DOI Listing

Novel Risk Loci Identified in a Genome-Wide Association Study of Urolithiasis in a Japanese Population.

J Am Soc Nephrol 2019 Apr 11. Epub 2019 Apr 11.

Laboratory of Clinical Genome Sequencing, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Tokyo, Japan;

Background: A family history of urolithiasis is associated with a more than doubling of urolithiasis risk, and a twin study estimating 56% heritability of the condition suggests a pivotal role for host genetic factors. However, previous genome-wide association studies (GWAS) have identified only six risk-related loci.

Methods: To identify novel urolithiasis-related loci in the Japanese population, we performed a large-scale GWAS of 11,130 cases and 187,639 controls, followed by a replication analysis of 2289 cases and 3817 controls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090942DOI Listing
April 2019
1 Read

Mortality in IgA Nephropathy: A Nationwide Population-Based Cohort Study.

J Am Soc Nephrol 2019 Apr 10. Epub 2019 Apr 10.

Department of Pediatrics, Örebro University Hospital, Örebro, Sweden.

The clinical course of IgA nephropathy (IgAN) varies from asymptomatic nonprogressive to aggressive disease, with up to one in four patients manifesting ESRD within 20 years of diagnosis. Although some studies have suggested that mortality appears to be increased in IgAN, such studies lacked matched controls and did not report absolute risk.

Methods: We conducted a population-based cohort study in Sweden, involving patients with biopsy-verified IgAN diagnosed in 1974-2011; main outcome measures were death and ESRD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018101017DOI Listing
April 2019
3 Reads

Thrombospondin Type 1 Domain-Containing 7A (THSD7A) Localizes to the Slit Diaphragm and Stabilizes Membrane Dynamics of Fully Differentiated Podocytes.

J Am Soc Nephrol 2019 Apr 10. Epub 2019 Apr 10.

Institutes of Cellular and Integrative Physiology and

Background: About 3%-5% of adults with membranous nephropathy have autoantibodies directed against thrombospondin type 1 domain-containing 7A (THSD7A), a podocyte-expressed transmembrane protein. However, the temporal and spatial expression of THSD7A and its biologic function for podocytes are unknown, information that is needed to understand the effects of THSD7A autoantibodies in this disease.

Methods: Using a variety of microscopic techniques, we analyzed THSD7A localization in postnatal, adult, and autoantibody-injected mice as well as in human podocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090941DOI Listing
April 2019
1 Read

Severe Arterial Hypertension from Cullin 3 Mutations Is Caused by Both Renal and Vascular Effects.

J Am Soc Nephrol 2019 Apr 9. Epub 2019 Apr 9.

Institut National de la Santé et de la Recherche Médicale U970, Paris Cardiovascular Research Center, Paris, France;

Background: Mutations in four genes, WNK lysine deficient protein kinase 1 and 4 ( and ), kelch like family member 3 (), or Cullin 3 (), can result in familial hyperkalemic hypertension (FHHt), a rare Mendelian form of human arterial hypertension. Although all mutations result in an increased abundance of WNK1 or WNK4, all FHHt-causing mutations, resulting in the skipping of exon 9, lead to a more severe phenotype.

Methods: We created and compared two mouse models, one expressing the mutant Cul3 protein ubiquitously () and the other specifically in vascular smooth muscle cells (). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2017121307DOI Listing
April 2019
2 Reads

GNAS: A New Nephrogenic Cause of Inappropriate Antidiuresis.

J Am Soc Nephrol 2019 Apr 8. Epub 2019 Apr 8.

Department of Renal Medicine, University College London, London, United Kingdom; and.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020143DOI Listing
April 2019
1 Read

Germline-Derived Gain-of-Function Variants of Gs-Coding Gene Identified in Nephrogenic Syndrome of Inappropriate Antidiuresis.

J Am Soc Nephrol 2019 Apr 8. Epub 2019 Apr 8.

Departments of Molecular Endocrinology,

Background: The stimulatory G-protein -subunit encoded by exons 1-13 (-Gs) mediates signal transduction of multiple G protein-coupled receptors, including arginine vasopressin receptor 2 (AVPR2). Various germline-derived loss-of-function -Gs variants of maternal and paternal origin have been found in pseudohypoparathyroidism type Ia and pseudopseudohypoparathyroidism, respectively. Specific somatic gain-of-function -Gs variants have been detected in McCune-Albright syndrome and may result in phosphate wasting. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018121268DOI Listing
April 2019
1 Read

How to Find a Resident Kidney Macrophage: the Single-Cell Sequencing Solution.

J Am Soc Nephrol 2019 Apr 4. Epub 2019 Apr 4.

Molecular Immunity Unit, Department of Medicine, University of Cambridge, Cambridge, UK;

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019030245DOI Listing

Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species.

J Am Soc Nephrol 2019 Apr 4. Epub 2019 Apr 4.

Department of Cell, Developmental, and Integrative Biology.

Background: Resident macrophages regulate homeostatic and disease processes in multiple tissues, including the kidney. Despite having well defined markers to identify these cells in mice, technical limitations have prevented identification of a similar cell type across species. The inability to identify resident macrophage populations across species hinders the translation of data obtained from animal model to human patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090931DOI Listing

Indoxyl Sulfate and p-Cresyl Sulfate Promote Vascular Calcification and Associate with Glucose Intolerance.

J Am Soc Nephrol 2019 Apr 2. Epub 2019 Apr 2.

Laboratory of Pathophysiology, Department of Biomedical Sciences.

Background: Protein-bound uremic toxins indoxyl sulfate (IS) and p-cresyl sulfate (PCS) have been associated with cardiovascular morbidity and mortality in patients with CKD. However, direct evidence for a role of these toxins in CKD-related vascular calcification has not been reported.

Methods: To study early and late vascular alterations by toxin exposure, we exposed CKD rats to vehicle, IS (150 mg/kg per day), or PCS (150 mg/kg per day) for either 4 days (short-term exposure) or 7 weeks (long-term exposure). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018060609DOI Listing
April 2019
1 Read

Inhibition of Sodium Glucose Cotransporter 2 Attenuates the Dysregulation of Kelch-Like 3 and NaCl Cotransporter in Obese Diabetic Mice.

J Am Soc Nephrol 2019 Mar 26. Epub 2019 Mar 26.

Division of Nephrology, Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan;

Background: Mechanisms underlying the frequent association between salt-sensitive hypertension and type 2 diabetes remain obscure. We previously found that protein kinase C (PKC) activation phosphorylates Kelch-like 3 (KLHL3), an E3 ubiquitin ligase component, at serine 433. We investigated whether impaired KLHL3 activity results in increased renal salt reabsorption NaCl cotransporter (NCC). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018070703DOI Listing

Authors' Reply.

J Am Soc Nephrol 2019 Mar 25. Epub 2019 Mar 25.

Urology Division, Renal Transplant Service and.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020124DOI Listing
March 2019
9.343 Impact Factor

Biphosphonate Therapy, Risk of Fracture, and Sites of Bone Mineral Density Assessments in Kidney Transplantation.

J Am Soc Nephrol 2019 Mar 25. Epub 2019 Mar 25.

Renal Physiology, APHP Hôpital Bichat, Paris, France and.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019010079DOI Listing

Mutations in Are Implicated in Steroid-Resistant Nephrotic Syndrome.

J Am Soc Nephrol 2019 Mar 25. Epub 2019 Mar 25.

Center for Genetic Medicine Research, Children's National Health System, Washington, DC;

Background: Studies have identified mutations in >50 genes that can lead to monogenic steroid-resistant nephrotic syndrome (SRNS). The gene, which encodes one of the protein components of the nuclear pore complex nucleoporin 160 kD (Nup160), is expressed in both human and mouse kidney cells. Knockdown of impairs mouse podocytes in cell culture. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018080786DOI Listing
March 2019
1 Read
9.343 Impact Factor

Filling the Gap: Nephrocytes as Model System in Kidney Research.

J Am Soc Nephrol 2019 Mar 25. Epub 2019 Mar 25.

INSERM UMR1163, Laboratory of Epithelial Biology and Disease, Imagine Institute, Paris Descartes University, Sorbonne Paris Cité, Hôpital Necker-Enfants Malades, Paris, France

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020181DOI Listing
March 2019
1 Read

Protein Phosphatase 1 Inhibitor-1 Mediates the cAMP-Dependent Stimulation of the Renal NaCl Cotransporter.

J Am Soc Nephrol 2019 Mar 22. Epub 2019 Mar 22.

Institute of Anatomy, University of Zurich, Zurich, Switzerland;

Background: A number of cAMP-elevating hormones stimulate phosphorylation (and hence activity) of the NaCl cotransporter (NCC) in the distal convoluted tubule (DCT). Evidence suggests that protein phosphatase 1 (PP1) and other protein phosphatases modulate NCC phosphorylation, but little is known about PP1's role and the mechanism regulating its function in the DCT.

Methods: We used mouse kidney preparations to test whether a DCT-enriched inhibitor of PP1, protein phosphatase 1 inhibitor-1 (I1), mediates cAMP's effects on NCC, and conducted yeast two-hybrid and coimmunoprecipitation experiments in NCC-expressing MDCK cells to explore protein interactions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018050540DOI Listing
March 2019
1 Read

Improving CKD-Specific Patient-Reported Measures of Health-Related Quality of Life.

J Am Soc Nephrol 2019 Apr 21;30(4):664-677. Epub 2019 Mar 21.

John Ware Research Group, Outcomes Measurement Department, Watertown, Massachusetts.

Background: Patient-reported outcome measures that are more practical and clinically useful are needed for patients with CKD. We compared a new CKD-specific quality-of-life impact scale (CKD-QOL) with currently used measures.

Methods: Patients (=485) in different treatment groups (nondialysis stages 3-5, on dialysis, or post-transplant) completed the kidney-specific CKD-QOL and Kidney Disease Quality of Life-36 (KDQOL-36) forms and the generic SF-12 Health Survey at baseline and 3 months. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018080814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442339PMC

Patient-Reported Outcomes: Toward Better Measurement of Patient-Centered Care in CKD.

J Am Soc Nephrol 2019 Apr 21;30(4):523-525. Epub 2019 Mar 21.

Department of Health Policy and Management, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland.

View Article

Download full-text PDF

Source
http://www.jasn.org/lookup/doi/10.1681/ASN.2019020169
Publisher Site
http://dx.doi.org/10.1681/ASN.2019020169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442338PMC
April 2019
2 Reads

Kidney Disease Quality of Life 36-Item Short Form Survey (KDQOL-36) Normative Values for the United States Dialysis Population and New Single Summary Score.

J Am Soc Nephrol 2019 Apr 21;30(4):654-663. Epub 2019 Mar 21.

Division of General Internal Medicine and Health Services Research, University of California Los Angeles, Los Angeles, California.

Background: The Kidney Disease Quality of Life 36-item short form survey (KDQOL-36) is a widely used, patient-reported outcome measure for patients on dialysis. Efforts to aid interpretation are needed.

Methods: We used a sample of 58,851 dialysis patients participating in the Medical Education Institute (MEI) KDQOL Complete program, and 443,947 patients from the US Renal Data System (USRDS) to develop the KDQOL-36 Summary Score (KSS) for the kidney-targeted KDQOL-36 scales (Burdens of Kidney Disease [BKD], Symptoms and Problems of Kidney Disease [SPKD], and Effects of Kidney Disease [EKD]). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018100994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442334PMC

Piecing Together the Risk of Sudden Cardiac Death on Dialysis.

Authors:
Eric D Weinhandl

J Am Soc Nephrol 2019 Apr 18;30(4):521-523. Epub 2019 Mar 18.

Pharmaceutical Care and Health Systems, University of Minnesota System, Minneapolis, Minnesota

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442352PMC
April 2019
1 Read

Comparative Cardiac Safety of Selective Serotonin Reuptake Inhibitors among Individuals Receiving Maintenance Hemodialysis.

J Am Soc Nephrol 2019 Apr 18;30(4):611-623. Epub 2019 Mar 18.

Division of Nephrology and Hypertension, Department of Medicine, University of North Carolina Kidney Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina.

Background: Individuals receiving maintenance hemodialysis may be particularly susceptible to the lethal cardiac consequences of drug-induced QT prolongation because they have a substantial cardiovascular disease burden and high level of polypharmacy, as well as recurrent exposure to electrolyte shifts during dialysis. Electrophysiologic data indicate that among the selective serotonin reuptake inhibitors (SSRIs), citalopram and escitalopram prolong the QT interval to the greatest extent. However, the relative cardiac safety of SSRIs in the hemodialysis population is unknown. Read More

View Article

Download full-text PDF

Source
http://www.jasn.org/lookup/doi/10.1681/ASN.2018101032
Publisher Site
http://dx.doi.org/10.1681/ASN.2018101032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442344PMC
April 2019
7 Reads

Archetype Analysis Identifies Distinct Profiles in Renal Transplant Recipients with Transplant Glomerulopathy Associated with Allograft Survival.

J Am Soc Nephrol 2019 Apr 14;30(4):625-639. Epub 2019 Mar 14.

Paris Translational Research Center for Organ Transplantation, Institut national de la santé et de la recherche médicale, Unité mixte de recherche-S970, Paris, France;

Background: Transplant glomerulopathy, a common glomerular lesion observed after kidney transplant that is associated with poor prognosis, is not a specific entity but rather the end stage of overlapping disease pathways. Its heterogeneity has not been precisely characterized to date.

Methods: Our study included consecutive kidney transplant recipients from three centers in France and one in Canada who presented with a diagnosis of transplant glomerulopathy (Banff cg score ≥1 by light microscopy), on the basis of biopsies performed from January of 2004 through December of 2014. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018070777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442337PMC
April 2019
3 Reads

The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells.

J Am Soc Nephrol 2019 Apr 13;30(4):564-576. Epub 2019 Mar 13.

Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital of Cologne, Cologne, Germany;

Background: RNA-binding proteins (RBPs) are fundamental regulators of cellular biology that affect all steps in the generation and processing of RNA molecules. Recent evidence suggests that regulation of RBPs that modulate both RNA stability and translation may have a profound effect on the proteome. However, regulation of RBPs in clinically relevant experimental conditions has not been studied systematically. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442340PMC
April 2019
2 Reads

Authors' Reply.

J Am Soc Nephrol 2019 Apr 13;30(4):714. Epub 2019 Mar 13.

Division of Nephrology, Department of Medicine and

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442336PMC

Macula Densa SGLT1-NOS1-Tubuloglomerular Feedback Pathway, a New Mechanism for Glomerular Hyperfiltration during Hyperglycemia.

J Am Soc Nephrol 2019 Apr 13;30(4):578-593. Epub 2019 Mar 13.

Department of Molecular Pharmacology and Physiology, College of Medicine.

Background: Glomerular hyperfiltration is common in early diabetes and is considered a risk factor for later diabetic nephropathy. We propose that sodium-glucose cotransporter 1 (SGLT1) senses increases in luminal glucose at the macula densa, enhancing generation of neuronal nitric oxide synthase 1 (NOS1)-dependent nitric oxide (NO) in the macula densa and blunting the tubuloglomerular feedback (TGF) response, thereby promoting the rise in GFR.

Methods: We used microperfusion, micropuncture, and renal clearance of FITC-inulin to examine the effects of tubular glucose on NO generation at the macula densa, TGF, and GFR in wild-type and macula densa-specific NOS1 knockout mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018080844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442354PMC
April 2019
1 Read

Complementary Roles for Single-Nucleus and Single-Cell RNA Sequencing in Kidney Disease Research.

J Am Soc Nephrol 2019 Apr 13;30(4):712-713. Epub 2019 Mar 13.

Department of Renal Medicine, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom; and

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442346PMC

The Other Glucose Transporter, SGLT1 - Also a Potential Trouble Maker in Diabetes?

J Am Soc Nephrol 2019 Apr 13;30(4):519-521. Epub 2019 Mar 13.

Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019020171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442345PMC

LRG1 Promotes Diabetic Kidney Disease Progression by Enhancing TGF--Induced Angiogenesis.

J Am Soc Nephrol 2019 Apr 11;30(4):546-562. Epub 2019 Mar 11.

Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York;

Background: Glomerular endothelial dysfunction and neoangiogenesis have long been implicated in the pathogenesis of diabetic kidney disease (DKD). However, the specific molecular pathways contributing to these processes in the early stages of DKD are not well understood. Our recent transcriptomic profiling of glomerular endothelial cells identified a number of proangiogenic genes that were upregulated in diabetic mice, including leucine-rich -2-glycoprotein 1 (LRG1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018060599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442349PMC
April 2019
2 Reads

Early Acute Microvascular Kidney Transplant Rejection in the Absence of Anti-HLA Antibodies Is Associated with Preformed IgG Antibodies against Diverse Glomerular Endothelial Cell Antigens.

J Am Soc Nephrol 2019 Apr 8;30(4):692-709. Epub 2019 Mar 8.

Paris Descartes, Sorbonne Paris Cité University, Paris, France;

Background: Although anti-HLA antibodies (Abs) cause most antibody-mediated rejections of renal allografts, non-anti-HLA Abs have also been postulated to contribute. A better understanding of such Abs in rejection is needed.

Methods: We conducted a nationwide study to identify kidney transplant recipients without anti-HLA donor-specific Abs who experienced acute graft dysfunction within 3 months after transplantation and showed evidence of microvascular injury, called acute microvascular rejection (AMVR). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018080868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442343PMC
April 2019
1 Read

DNMT1 in Progenitor Cells Is Essential for Transposable Element Silencing and Kidney Development.

J Am Soc Nephrol 2019 Apr 8;30(4):594-609. Epub 2019 Mar 8.

Renal-Electrolyte and Hypertension Division, Department of Medicine,

Background: Cytosine methylation of regulatory regions, such as promoters and enhancers, plays a key role in regulating gene expression, however, its role in kidney development has not been analyzed.

Methods: To identify functionally important epigenome-modifying enzymes and genome regions where methylation modifications are functionally important for kidney development, we performed genome-wide methylation analysis, expression profiling, and systematic genetic targeting of DNA methyltransferases (, , and ) and Ten-eleven translocation methylcytosine hydroxylases () in nephron progenitor cells () in mice.

Results: Genome-wide methylome analysis indicated dynamic changes on promoters and enhancers during development. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018070687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442333PMC
April 2019
4 Reads

Effects of Hemodiafiltration versus Conventional Hemodialysis in Children with ESKD: The HDF, Heart and Height Study.

J Am Soc Nephrol 2019 Apr 7;30(4):678-691. Epub 2019 Mar 7.

Nephrology Unit, Center for Pediatrics and Adolescent Medicine, Heidelberg, Germany.

Background: Hypertension and cardiovascular disease are common in children undergoing dialysis. Studies suggest that hemodiafiltration (HDF) may reduce cardiovascular mortality in adults, but data for children are scarce.

Methods: The HDF, Heart and Height study is a nonrandomized observational study comparing outcomes on conventional hemodialysis (HD) versus postdilution online HDF in children. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018100990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442347PMC
April 2019
1 Read
9.343 Impact Factor

Single-Cell RNA Profiling of Glomerular Cells Shows Dynamic Changes in Experimental Diabetic Kidney Disease.

J Am Soc Nephrol 2019 Apr 7;30(4):533-545. Epub 2019 Mar 7.

Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York;

Background: Recent single-cell RNA sequencing (scRNA-seq) analyses have offered much insight into cell-specific gene expression profiles in normal kidneys. However, in diseased kidneys, understanding of changes in specific cells, particularly glomerular cells, remains limited.

Methods: To elucidate the glomerular cell-specific gene expression changes in diabetic kidney disease, we performed scRNA-seq analysis of isolated glomerular cells from streptozotocin-induced diabetic endothelial nitric oxide synthase (eNOS)-deficient (eNOS) mice and control eNOS mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442341PMC
April 2019
4 Reads

Expanding the Patient's Voice in Nephrology with Patient-Reported Outcomes.

J Am Soc Nephrol 2019 Apr 7;30(4):530-532. Epub 2019 Mar 7.

Division of General Internal Medicine and Health Services Research, University of California, Los Angeles, Los Angeles, California.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019010019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442351PMC

Efficacy and Safety of Tenapanor in Patients with Hyperphosphatemia Receiving Maintenance Hemodialysis: A Randomized Phase 3 Trial.

J Am Soc Nephrol 2019 Apr 7;30(4):641-652. Epub 2019 Mar 7.

Division of Nephrology, Stanford University School of Medicine, Stanford, California.

Background: Guidelines recommend reducing elevated serum phosphate in patients with CKD. Tenapanor, a minimally absorbed inhibitor of gastrointestinal sodium/hydrogen exchanger 3 (NHE3), reduces paracellular phosphate transport.

Methods: In this phase 3 randomized, double-blind trial, we randomly assigned patients with hyperphosphatemia receiving maintenance hemodialysis to receive twice-daily oral tenapanor (3, 10, or 30 mg [the latter down-titrated, if needed]) for 8 weeks. Read More

View Article

Download full-text PDF

Source
http://www.jasn.org/lookup/doi/10.1681/ASN.2018080832
Publisher Site
http://dx.doi.org/10.1681/ASN.2018080832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442342PMC
April 2019
30 Reads

Staying the Course: Through End of Life in ESRD.

J Am Soc Nephrol 2019 Feb 19. Epub 2019 Feb 19.

Internal Medicine/Nephrology, University of Michigan, Ann Arbor, Michigan

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019010020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405150PMC
February 2019

Lower Extremity Amputation and Health Care Utilization in the Last Year of Life among Medicare Beneficiaries with ESRD.

J Am Soc Nephrol 2019 Feb 19. Epub 2019 Feb 19.

Division of Nephrology, Department of Medicine and the Kidney Research Institute, University of Washington, Seattle, Washington.

Background: Lower extremity amputation is common among patients with ESRD, and often portends a poor prognosis. However, little is known about end-of-life care among patients with ESRD who undergo amputation.

Methods: We conducted a mortality follow-back study of Medicare beneficiaries with ESRD who died in 2002 through 2014 to analyze patterns of lower extremity amputation in the last year of life compared with a parallel cohort of beneficiaries without ESRD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018101002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405144PMC
February 2019
1 Read

Overall and Site-Specific Cancer Mortality in Patients on Dialysis and after Kidney Transplant.

J Am Soc Nephrol 2019 Feb 14. Epub 2019 Feb 14.

School of Public Health, University of Sydney, Sydney, Australia.

Patients with ESRD have a substantially increased cancer risk, but few studies have examined the patterns of cancer mortality along a patient's journey from dialysis to transplantation.

Methods: We identified all Australian patients on dialysis and patients with transplants from 1980 to 2014 from the Australia and New Zealand Dialysis and Transplant Registry. Using standardized mortality ratios (SMRs), we compared cancer mortality among patients on dialysis and patients with transplants versus the general population (overall and by age, sex, year, and site); we also performed a subgroup analysis excluding patients with preexisting cancers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405152PMC
February 2019
30 Reads
9.343 Impact Factor

Renal Perfusion and Renal Nerve Activity.

J Am Soc Nephrol 2019 Apr 13;30(4):711. Epub 2019 Feb 13.

Adult Nephrology, Shaare Zedek Medical Center, Jerusalem, Israel

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018121226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442353PMC

Long-Range Chromatin Interactions in the Kidney.

J Am Soc Nephrol 2019 Feb 13. Epub 2019 Feb 13.

Renal-Electrolyte and Hypertension Division, Department of Medicine and

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019010044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405157PMC
February 2019

Authors' Reply.

J Am Soc Nephrol 2019 Apr 13;30(4):711. Epub 2019 Feb 13.

Department of Nuclear Medicine, Physiology and PET, Rigshospitalet, Glostrup, Denmark.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019010049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442348PMC

Integrated Functional Genomic Analysis Enables Annotation of Kidney Genome-Wide Association Study Loci.

J Am Soc Nephrol 2019 Feb 13. Epub 2019 Feb 13.

Department of Anatomic Pathology,

Background: Linking genetic risk loci identified by genome-wide association studies (GWAS) to their causal genes remains a major challenge. Disease-associated genetic variants are concentrated in regions containing regulatory DNA elements, such as promoters and enhancers. Although researchers have previously published DNA maps of these regulatory regions for kidney tubule cells and glomerular endothelial cells, maps for podocytes and mesangial cells have not been available. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018030309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405142PMC
February 2019
1 Read

Spliced XBP1 Rescues Renal Interstitial Inflammation Due to Loss of in Collecting Ducts.

J Am Soc Nephrol 2019 Feb 11. Epub 2019 Feb 11.

Departments of Internal Medicine and

Background: encodes a resident protein in the endoplasmic reticulum membrane that, when mutated, causes human autosomal dominant polycystic liver disease. Selective inactivation of in all distal nephron segments in embryonic mouse kidney results in polycystin-1-mediated polycystic kidney disease (PKD). It also activates the Ire1-Xbp1 branch of the unfolded protein response, producing Xbp1s, the active transcription factor promoting expression of specific genes to alleviate endoplasmic reticulum stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018060614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405156PMC
February 2019
1 Read
9.343 Impact Factor

Treatment with 2,4-Dihydroxybenzoic Acid Prevents FSGS Progression and Renal Fibrosis in Podocyte-Specific Knockout Mice.

J Am Soc Nephrol 2019 Feb 8. Epub 2019 Feb 8.

Division of Nephrology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts;

Background: Although studies have identified >55 genes as causing steroid-resistant nephrotic syndrome (SRNS) and localized its pathogenesis to glomerular podocytes, the disease mechanisms of SRNS remain largely enigmatic. We recently reported that individuals with mutations in COQ6, a coenzyme Q (also called CoQ, CoQ, or ubiquinone) biosynthesis pathway enzyme, develop SRNS with sensorineural deafness, and demonstrated the beneficial effect of CoQ for maintenace of kidney function.

Methods: To study function in podocytes, we generated a podocyte-specific knockout mouse ( ) model and a transient siRNA-based knockdown in a human podocyte cell line. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018060625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405149PMC
February 2019
7 Reads

Iron, Hepcidin, and Death in Human AKI.

J Am Soc Nephrol 2019 Feb 8. Epub 2019 Feb 8.

Division of Renal Medicine, Brigham and Women's Hospital, Boston, Massachusetts.

Background: Iron is a key mediator of AKI in animal models, but data on circulating iron parameters in human AKI are limited.

Methods: We examined results from the ARF Trial Network study to assess the association of plasma catalytic iron, total iron, transferrin, ferritin, free hemoglobin, and hepcidin with 60-day mortality. Participants included critically ill patients with AKI requiring RRT who were enrolled in the study. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018100979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405140PMC
February 2019
5 Reads
9.343 Impact Factor

Cardiopulmonary Resuscitation in Outpatient Dialysis Clinics: Perception of Futility?

J Am Soc Nephrol 2019 Feb 7. Epub 2019 Feb 7.

Division of Nephrology, University Health Network, Toronto, Ontario, Canada

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2019010046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405143PMC
February 2019
1 Read

Outcomes for Hemodialysis Patients Given Cardiopulmonary Resuscitation for Cardiac Arrest at Outpatient Dialysis Clinics.

J Am Soc Nephrol 2019 Feb 7. Epub 2019 Feb 7.

Duke Clinical Research Institute.

Background: Out-of-hospital cardiac arrest, the leading cause of death among patients on hemodialysis, occurs frequently within outpatient dialysis centers. Practice guidelines recommend resuscitation training for all dialysis clinic staff and on-site defibrillator availability, but the extent of staff involvement in cardiopulmonary resuscitation (CPR) efforts and its association with outcomes is unknown.

Methods: We used data from the Cardiac Arrest Registry to Enhance Survival and the Centers for Medicare & Medicaid Services dialysis facility database to identify patients who had cardiac arrest within outpatient dialysis clinics between 2010 and 2016 in the southeastern United States. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018090911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405155PMC
February 2019
1 Read

Systemic Succinate Homeostasis and Local Succinate Signaling Affect Blood Pressure and Modify Risks for Calcium Oxalate Lithogenesis.

J Am Soc Nephrol 2019 Feb 6. Epub 2019 Feb 6.

Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel;

Background: In the kidney, low urinary citrate increases the risk for developing kidney stones, and elevation of luminal succinate in the juxtaglomerular apparatus increases renin secretion, causing hypertension. Although the association between stone formation and hypertension is well established, the molecular mechanism linking these pathophysiologies has been elusive.

Methods: To investigate the relationship between succinate and citrate/oxalate levels, we assessed blood and urine levels of metabolites, renal protein expression, and BP (using 24-hour telemetric monitoring) in male mice lacking slc26a6 (a transporter that inhibits the succinate transporter NaDC-1 to control citrate absorption from the urinary lumen). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1681/ASN.2018030277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405146PMC
February 2019
4 Reads