1,100 results match your criteria Journal of the American Association for Laboratory Animal Science : JAALAS[Journal]


Retrospective Analysis of the Effects of Identification Procedures and Cage Changing by Using Data from Automated, Continuous Monitoring.

J Am Assoc Lab Anim Sci 2019 Feb 14. Epub 2019 Feb 14.

Many variables can influence animal behavior and physiology, potentially affecting scientific study outcomes. Laboratoryand husbandry procedures-including handling, cage cleaning, injections, blood collection, and animal identification-mayproduce a multitude of effects. Previous studies have examined the effects of such procedures by making behavioral andphysiologic measurements at specific time points; this approach can be disruptive and limits the frequency or duration ofobservations. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000056DOI Listing
February 2019

Survey of Behavioral Indices of Welfare in Research Chimpanzees (Pan troglodytes) in the United States.

J Am Assoc Lab Anim Sci 2019 Feb 14. Epub 2019 Feb 14.

Chimpanzees demand specialized housing and care and the highest degree of attention to animal welfare. The current project used a survey method to collate information on chimpanzee housing and behavioral indices of welfare across all 6 of the chimpanzee research facilities in the United States. Data were compiled on 701 chimpanzees ranging from 2 to 62 y old (mean age, 26. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000034DOI Listing
February 2019
5 Reads

Head Tilt in Immunodeficient Mice Due to Contamination of Drinking Water by Burkholderia gladioli.

J Am Assoc Lab Anim Sci 2019 Feb 14. Epub 2019 Feb 14.

Immunodeficient mice in multiple holding rooms presented with head tilt, circling, spinning when picked up by the tail,dehydration, and lethargy. Burkholderia gladioli, a plant pathogen, was identified as the causative agent. Environmental testing revealed the presence of B. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000106DOI Listing
February 2019

Influence of Rater Training on Inter- and Intrarater Reliability When Using the Rat Grimace Scale.

J Am Assoc Lab Anim Sci 2019 Feb 12. Epub 2019 Feb 12.

Rodent grimace scales facilitate assessment of ongoing pain. Reported rater training using these scales varies considerablyand may contribute to the observed variability in interrater reliability. This study evaluated the effect of training on interrater reliability with the Rat Grimace Scale (RGS). Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000044DOI Listing
February 2019

Ammonia Measurement in the IVC Microenvironment.

J Am Assoc Lab Anim Sci 2019 Jan 31. Epub 2019 Jan 31.

In this review, we describe the methods and technology used to measure intracage ammonia levels; the data were derivedfrom 38 articles published since 1970. Ammonia concentration is commonly used as a surrogate for assessing environmentalquality inside rodent cages. Data generated from this group of publications have been used to support new husbandry practices, determine the effect of ammonia on health, and establish the effectiveness of caging systems. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000006DOI Listing
January 2019

Effects of Various Commercially Available Enrichment Options on Handling and Chronic Stress Markers in Female ICR Mice.

J Am Assoc Lab Anim Sci 2019 Jan 29. Epub 2019 Jan 29.

Although social housing of mice generally is preferred, mice must be individually housed in some situations. In these cases, enhanced attention to environmental enrichment is encouraged, but few studies assess the wellbeing of mice provided various enrichments. In this study, we used female ICR mice to evaluate enrichment strategies that encouraged natural behaviorsincluding foraging, exercise, sheltering, and socialization. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000103DOI Listing
January 2019
4 Reads

The Likelihood of Misidentifying Rodent Pasteurellaceae by Using Results from a Single PCR Assay.

J Am Assoc Lab Anim Sci 2019 Jan 16. Epub 2019 Jan 16.

The precise identification of rodent Pasteurellaceae is known to be highly challenging. An unknown strain of Pasteurellaceaeappeared and rapidly spread throughout our animal facilities. Standard microbiology, combined with biochemical analysis, suggested that the bacteria strain was Rodentibacter pneumotropicus or R. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000049DOI Listing
January 2019

Chemical Contaminants from Plastics in the Animal Environment.

J Am Assoc Lab Anim Sci 2019 Jan 15. Epub 2019 Jan 15.

Accidental exposure of our mice to bisphenol A (BPA) from damaged polycarbonate cages 20 y ago provided some of thefirst evidence of the harmful effects of exposure to this common chemical. Recently we found that housing mice in damagedpolysulfone cages resulted in similar harmful effects due to exposure to bisphenol S (BPS). This problem was unexpected for2 reasons. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000074DOI Listing
January 2019
1 Read
1.118 Impact Factor

Regional Anesthesia for Dentistry and Orofacial Surgery in Rhesus Macaques (Macaca mulatta).

J Am Assoc Lab Anim Sci 2019 Jan 10. Epub 2019 Jan 10.

Regional anesthesia is a commonly used adjunct to orofacial dental and surgical procedures in companion animals andhumans. However, appropriate techniques for anesthetizing branches of the mandibular and maxillary nerves have not beendescribed for rhesus monkeys. Skulls of 3 adult rhesus monkeys were examined to identify relevant foramina, establish appropriate landmarks for injection, and estimate injection angles and depth. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000068DOI Listing
January 2019

Capnography-guided Endotracheal Intubation as an Alternative to Existing Intubation Methods in Rabbits.

J Am Assoc Lab Anim Sci 2019 Jan 9. Epub 2019 Jan 9.

Rabbits provide a unique challenge for routine endotracheal intubation in clinical practice because of various distinctiveanatomic and physiologic features. Many previously proposed methods for endotracheal intubation in rabbits are limited byseveral factors, including the needs for expensive equipment and high levels of technical expertise. We evaluated capnographyfor its effectiveness in assisting endotracheal intubation in rabbits. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000150DOI Listing
January 2019
1 Read

Evaluation of 4 Presurgical Skin Preparation Methods in Mice.

J Am Assoc Lab Anim Sci 2019 Jan 4;58(1):71-77. Epub 2019 Jan 4.

Division of Animal Resources, Emory University, Atlanta, Georgia, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia.

Mice routinely undergo surgical procedures for use in research; however, studies of skin preparation methods to achieve antisepsis are rare. The present study evaluated 4 skin preparation treatments: depilatory agent followed by povidone-iodine and alcohol scrub; depilatory agent followed by povidone-iodine and saline scrub; electric clippers followed by povidone-iodine and alcohol scrub; and electric clippers followed by povidone-iodine and saline scrub. Swabs for bacterial culture were obtained immediately after hair removal and after scrubbing to measure the reduction in bacterial load. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351048PMC
January 2019
1 Read

Ratio of the Bronchial Lumen to Pulmonary Artery Diameter in Rhesus Macaques () without Clinical Pulmonary Disease.

J Am Assoc Lab Anim Sci 2019 Jan 27;58(1):83-86. Epub 2018 Dec 27.

Department of Surgery and Radiology, University of Tehran, Tehran, Iran.

In human medicine, CT is widely used to detect changes in bronchial luminal diameter. The diameter of the artery that runs adjacent to the bronchus does not change dramatically along the airway path, such that this artery can be used as a reference to detect changes in the bronchial luminal diameter. The bronchoarterial ratio is increasingly used in veterinary medicine for the detection of lower airway diseases in animals. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351050PMC
January 2019
1 Read

Alfaxalone-Xylazine Anesthesia in Laboratory Mice ().

J Am Assoc Lab Anim Sci 2019 Jan 20;58(1):30-39. Epub 2018 Dec 20.

University Laboratory Animal Resources, University of Pennsylvania, Philadelphia, Pennsylvania, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania;, Email:

Since its recent reformulation, alfaxalone has gained popularity as an injectable veterinary anesthetic, including promising studies demonstrating the use of alfaxalone-xylazine for anesthesia in mice. Here we sought to expand these studies by testing additional dose ranges, elaborating on physiologic monitoring, testing sex- and strain-associated differences, and evaluating efficacy during actual surgical conditions. C57BL/6J mice showed significant sex-associated differences in anesthetic sensitivity, with males requiring higher doses of alfaxalone (80-120 mg/kg IP alfaxalone with 10 mg/kg IP xylazine) than females (40-80 mg/kg IP alfaxalone with 10 mg/kg IP xylazine) to achieve a surgical plane of anesthesia. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351053PMC
January 2019

Comparison of Isoflurane, Ketamine-Dexmedetomidine, and Ketamine-Xylazine for General Anesthesia during Oral Procedures in Rice Rats ().

J Am Assoc Lab Anim Sci 2019 Jan 20;58(1):40-49. Epub 2018 Dec 20.

Department of Physiological Sciences, University of Florida, Gainesville, Florida;, Email:

Rice rats () are an unconventional laboratory species that has been used to study photoperiodicity, periodontitis, and osteonecrosis of the jaw. Interventional procedures that require anesthesia, including oral procedures, are sometimes necessary in preclinical settings. The use of anesthetics including isoflurane and ketamine combined with α2-adrenoreceptor agonists, such as dexmedetomidine and xylazine, is well-established for laboratory rodents. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351055PMC
January 2019
1 Read
1.118 Impact Factor

Effects of General Anesthesia on 2 Urinary Biomarkers of Kidney Injury-Hepatitis A Virus Cellular Receptor 1 and Lipocalin 2-in Male C57BL/6J Mice.

J Am Assoc Lab Anim Sci 2019 Jan 11;58(1):21-29. Epub 2018 Dec 11.

Department of Comparative Medicine, Penn State University College of Medicine, Milton S Hershey Medical Center, Hershey, Pennsylvania.

Urinary biomarkers are used increasingly for sensitive prediction of kidney injury in preclinical and clinical studies. Given the frequent requirement of anesthesia in various animal models of disease, it is important to define the effects of anesthesia on kidney injury biomarkers to guide the appropriate selection of anesthetic agents and to avoid potential confounders in the interpretation of data. Therefore, we performed a prospective study using male C57BL/6J mice ( = 45) exposed to a single anesthetic episode to determine the effects several common anesthesia regimens on the urinary excretion of 2 commonly used kidney injury biomarkers: hepatitis A virus cellular receptor 1 (HAVCR1, also known as KIM1) and lipocalin 2 (LCN2, also known as NGAL). Read More

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https://www.ingentaconnect.com/content/10.30802/AALAS-JAALAS
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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351046PMC
January 2019
1 Read

Daily Water Intake by Common Marmosets () and Recommendations Regarding Fluid Regulation.

J Am Assoc Lab Anim Sci 2019 Jan 11;58(1):16-20. Epub 2018 Dec 11.

Division of Comparative Medicine;, Email:

The typical daily water intake of common marmosets () in a research setting has not been well characterized. Because these New World primates are in demand as animal models for neurobehavioral experiments, which can include the potential use of fluid regulation for training, veterinary and research staff need to understand how marmosets keep hydrated under normal circumstances. In the current study, we measured the water consumption of older (age, 5 to 12 y; = 11) and younger (age, 1 to 2 y; = 11) marmosets every 3 h during the 12-h light phase in 2 different months (January and July). Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351047PMC
January 2019

Wellbeing of Alcohol-preferring Rats Euthanized with Carbon Dioxide at Very Low and Low Volume Displacement Rates.

Authors:
Debra L Hickman

J Am Assoc Lab Anim Sci 2019 Jan 7;58(1):78-82. Epub 2018 Dec 7.

Laboratory Animal Resource Center, School of Medicine, Indiana University, Indianapolis, Illinois;, Email:

The 2013 recommend the use of very-low or low flow rates of 100% carbon dioxide to euthanize small rodents. Although inhalation of high concentrations of carbon dioxide are generally recognized as painful in humans, whether the use of these low-flow methods of euthanasia increase potential distress for rats is unclear. This study compared physiologic and behavioral markers of animal wellbeing for rats euthanized by using 10% volume displacement per minute (VD/min), 30% VD/min, and 70% VD/min of 100% carbon dioxide. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351044PMC
January 2019

3D-printed Wash Station with Integrated Anesthesia Delivery Manifold for High-throughput Depilation of Laboratory Mice.

J Am Assoc Lab Anim Sci 2019 Jan 7;58(1):65-70. Epub 2018 Dec 7.

Department of Biological Sciences, Notre Dame Integrated Imaging Facility, Harper Cancer Research Center, University of Notre Dame, Notre Dame, Indiana;, Email:

Depilation (that is, hair removal) is a necessary prerequisite for many small animal surgeries and optical imaging experiments. Over-the-counter depilatory creams are widely used, owing to their efficacy, safety, and low rates of skin irritation and infection. However, the use of these creams is generally messy and time-consuming and generates considerable waste. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351045PMC
January 2019

Anesthesia and Euthanasia of Brine Shrimp ().

J Am Assoc Lab Anim Sci 2019 Jan 29;58(1):58-64. Epub 2018 Nov 29.

Division of Comparative Medicine, Vanderbilt University Medical Center, Nashville, Tennessee; Translational Pathology Shared Resource, Vanderbilt University Medical Center, Nashville, Tennessee.

Invertebrates are often overlooked as laboratory animals, yet they are commonly used in toxicology, developmental, cellular and molecular biology, and radiation studies with euthanasia as an endpoint. Little is known regarding appropriate euthanasia methods for invertebrate species, particularly for . Here, we evaluated the AVMA-recommended 2-step method of euthanasia in brine shrimp (). Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351054PMC
January 2019

Effects of Intrauterine Air Bubbles on Embryonic Development in Mice.

J Am Assoc Lab Anim Sci 2019 Jan 29;58(1):7-15. Epub 2018 Nov 29.

College of Animal Science and Technology, Hebei Agricultural University, Baoding, Hebei, China;, Email:

During murine embryo transfer, air bubbles frequently are loaded with embryos into the transfer catheter, but the role of air bubbles on embryonic development is unclear. This study shows that intrauterine air disrupted embryo spacing, induced deciduoma, and impaired postimplantation development. RNA sequencing showed that the gene expression profile of air-induced deciduoma differed significantly from that of embryo-induced decidua but is similar to tetraploid-induced deciduoma. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351051PMC
January 2019
2 Reads

Validation of an Autoclave Procedure for Sterilization of Mouse () Carcasses.

J Am Assoc Lab Anim Sci 2019 Jan 6;58(1):87-91. Epub 2018 Nov 6.

Central Animal Facility, University Medical Center Goettingen, Georg-August University, Goettingen, Germany.

The sterilization of potentially infectious animal carcasses is an important biologic safety issue in animal facilities operating as infection or quarantine barriers. However, the literature lacks a validated protocol. Here we describe the validation of an autoclave program suitable for daily use in a small rodent biocontainment unit. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351042PMC
January 2019

Comparison of Dexmedetomidine-Ketamine-Midazolam and Isoflurane for Anesthesia of Black-tailed Prairie Dogs ().

J Am Assoc Lab Anim Sci 2019 Jan 5;58(1):50-57. Epub 2018 Nov 5.

Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.

Few studies evaluate anesthesia in black-tailed prairie dogs (). Isoflurane inhalant anesthesia is used in this species most commonly, but injectable protocols are poorly described. Here we compared the physiologic effects, including anesthetic depth, vital signs, and hematologic changes, of anesthetic protocols using isoflurane or a combination of dexmedetomidine, ketamine, and midazolam in black-tailed prairie dogs. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351049PMC
January 2019
5 Reads

Acrylamide Production in Autoclaved Rodent Feed.

J Am Assoc Lab Anim Sci 2018 Oct 25. Epub 2018 Oct 25.

Sterilization of rodent feed by steam autoclaving is a common practice in many research institutions. Often we only considerthe beneficial effects of this process-the reduction of microbial contamination-and forget that the high temperatures andpressures can have negative effects on diet quality. The purpose of our study was to assess both the physical and chemicalchanges to a standard rodent feed autoclaved at multiple sterilization temperatures and the effects of the treated diets on mice. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241378PMC
October 2018

Effects of Music Enrichment on Individually Housed Male New Zealand White Rabbits.

J Am Assoc Lab Anim Sci 2018 Oct 25. Epub 2018 Oct 25.

The beneficial effect of music has been demonstrated in many species. Although commercially available music CDs intended for use with rabbits are available, these enrichments have not been critically evaluated to determine whether they reduce distress. In this study, we used an aging colony of male rabbits to evaluate how the provision of music enrichment affected the wellbeing of the colony. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241384PMC
October 2018

Body Condition Scoring for Adult Zebrafish (Danio rerio).

J Am Assoc Lab Anim Sci 2018 Oct 25. Epub 2018 Oct 25.

Body condition scoring (BCS) is a simple, rapid, noninvasive tool used to assess body condition in animals. In this study, wedeveloped and validated a diagram-based BCS for adult zebrafish (Danio rerio), a popular research model. After receiving 20min of hands-on training regarding the scoring system, 5 people each rated 95 adult zebrafish. Read More

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http://www.ingentaconnect.com/content/10.30802/AALAS-JAALAS-
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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241379PMC
October 2018
11 Reads

Preventing Adverse Events at Research Facilities.

Authors:
V Wensley Koch

J Am Assoc Lab Anim Sci 2018 Oct 22. Epub 2018 Oct 22.

In this article, adverse events are defined as events that lead to significant injury or illness, unrelieved pain or distress, orthe death of an animal, excluding those caused by IACUC-approved research procedures. The ability to identify possible adverse events is an essential step in planning for risk prevention and mitigation. Using data from news sources and the Animal and Plant Health Inspection Service (APHIS) website, this article provides guidance to research facilities regarding various types of adverse events to consider in risk management plans because these events might occur or have occurred and, in some cases, have been documented as noncompliances at APHIS-regulated research facilities. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241386PMC
October 2018

Sterilization of Silastic Capsules Containing 17β-Estradiol for Effective Hormone Delivery in Mus musculus.

J Am Assoc Lab Anim Sci 2018 Oct 12. Epub 2018 Oct 12.

Silastic capsules are frequently used to study the physiologic effects of estrogen exposure in animal models. The Officeof Laboratory Animal Welfare requires the sterilization of nonpharmaceutical-grade compounds before use. We compared 2commonly used terminal sterilization methods-ionizing radiation (IR) and ethylene oxide (EO)-for their utility in sterilizingsilastic capsules containing 0. Read More

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http://www.ingentaconnect.com/content/10.30802/AALAS-JAALAS-
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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241387PMC
October 2018
1 Read

Using Hysterectomy Rederivation to Produce Guinea Pigs (Cavia porcellus) Free of Guinea Pig Cytomegalovirus.

J Am Assoc Lab Anim Sci 2018 Oct 10. Epub 2018 Oct 10.

Due to similarities in placentation, guinea pigs can be used as models of human cytomegalovirus infection, but they mustbe free of guinea pig cytomegalovirus. Many commercial guinea pig colonies are enzootically infected with guinea pigcytomegalovirus, which can be transmitted vertically as well as horizontally through saliva, vaginal secretions, and milk.These characteristics make its eradication in a commercial setting challenging. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241382PMC
October 2018
1 Read

Home Cage Compared with Induction Chamber for Euthanasia of Laboratory Rats.

Authors:
Debra L Hickman

J Am Assoc Lab Anim Sci 2018 Oct 10. Epub 2018 Oct 10.

This study compared behavioral and physiologic changes in Sprague-Dawley and Brown Norway rats that were euthanizedby using a 30% volume displacement rate of CO2 in either their home cage or an induction chamber; rats euthanized in thehome cage were hypothesized to demonstrate a higher level of animal wellbeing. No significant differences were detectedin the physiologic responses to home cage versus induction chamber euthanasia groups. A few strain-related behavioraldifferences occurred. Read More

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http://www.ingentaconnect.com/content/10.30802/AALAS-JAALAS-
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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241385PMC
October 2018
12 Reads

Interpreting Neuroendocrine Hormones, Corticosterone, and Blood Glucose to Assess the Wellbeing of Anesthetized Rats during Euthanasia.

Authors:
Debra L Hickman

J Am Assoc Lab Anim Sci 2018 Oct 10. Epub 2018 Oct 10.

Current recommendations for assessing animal wellbeing during euthanasia suggest that measuring neuroendocrine hormones-such as ACTH, noradrenaline, and adrenaline-is preferable to measuring corticosterone and blood glucose because of the sensitivity of neuroendocrine hormones to the acute stress associated with rapid methods of euthanasia. However, theseneuroendocrine hormones can be stimulated in ways that confound interpretation of welfare assessment in euthanasia studies.Although this property does not negate the usefulness of neuroendocrine hormones as tools of assessment, it is importantto differentiate the stress associated with the induction of anesthesia before the loss of consciousness (an animal wellbeingconcern) with the physiologic responses that occur after the loss of consciousness (not an animal wellbeing concern). Read More

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http://www.ingentaconnect.com/content/10.30802/AALAS-JAALAS-
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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241377PMC
October 2018
1 Read

Abstracts of Scientific Presentations 2018 AALAS National Meeting Baltimore, Maryland.

Authors:

J Am Assoc Lab Anim Sci 2018 Sep;57(5):534-642

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159684PMC
September 2018
4 Reads

Reference Intervals for Plasma Biochemical Variables by Point-of-Care Testing in Captive Black-tailed Prairie Dogs (Cynomys ludovicianus).

J Am Assoc Lab Anim Sci 2018 Sep 12. Epub 2018 Sep 12.

Black-tailed prairie dogs (Cynomys ludovicianus) are kept in zoological collections, maintained as companion pets, and aretested in field and laboratory settings. Biochemical analysis for routine health and research purposes can be performed byusing point-of-care (POC) testing; however, analyzer- and species-specific reference intervals need to be determined. In this prospective study, 50 captive-raised sexually intact prairie dogs (16 females, 34 males) underwent plasma biochemical analysisby using a veterinary POC biochemical analyzer. Read More

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http://www.ingentaconnect.com/content/10.30802/AALAS-JAALAS-
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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241388PMC
September 2018
4 Reads

Effects of Laboratory Animal Science Training on Scientists' Attitudes and Practice in Egypt.

J Am Assoc Lab Anim Sci 2018 Sep 12. Epub 2018 Sep 12.

The implementation of principles and guidelines that govern the various areas of research in an educational institution isone of the key factors in international recognition of its research integrity and value. The privilege of conducting research using animal subjects requires adherence to international regulations and standards governing the humane care and use of laboratory animals. The IACUC at our university deemed it critical to have an animal care and use training program to raise researchers' understanding and knowledge. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241381PMC
September 2018
8 Reads
1.118 Impact Factor

Evaluation of Infrared Thermography for Temperature Measurement in Adult Male NMRI Nude Mice.

J Am Assoc Lab Anim Sci 2018 Sep 12. Epub 2018 Sep 12.

Temperature monitoring during critical care provides important data required to guide treatment delivery. Body temperatureis an easily quantified clinical parameter that can yield much information concerning the health of an animal. In researchsettings, temperature has been adopted as a means to judge humane endpoints. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241389PMC
September 2018
16 Reads

Effects of Extruded Compared with Pelleted Diets on Laboratory Mice Housed in Individually Ventilated Cages and the Cage Environment.

J Am Assoc Lab Anim Sci 2018 Sep 5. Epub 2018 Sep 5.

The physical form of the diet fed to laboratory animals should be evaluated to reduce experimental variations and confoundingfactors. This 14-d study evaluated the effects of diet form (pelleted or extruded) on intracage ammonia concentrations,feed disappearance, body weight, cage weight, and the degree of cage soilage and whether these effects were influenced bystrain or stock or sex. Mice (C57BL/6, ICR, and nude; age, 4 wk) were randomly assigned to 4 treatment groups representingpelleted and extruded diets from each of 2 vendors (pelleted diet groups, P1 and P2; extruded diet groups, E1 and E2). Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241380PMC
September 2018
5 Reads

Ivermectin-compounded Feed Compared with Topical Moxidectin-Imidacloprid for Eradication of in Laboratory Mice.

J Am Assoc Lab Anim Sci 2018 Sep 5;57(5):483-497. Epub 2018 Sep 5.

Tri-Institutional Training Program in Laboratory Animal Medicine and Science, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, New York, Center for Comparative Medicine and Pathology, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, New York;, Email:

Demodex musculi is a prostigmatid follicular mite that has rarely been reported in laboratory mice. Although prevalence of this species has not been assessed formally, we have found that many imported mouse strains from noncommercial sources harbor Demodex mites. To assess whether an acaricide can be used to eradicate this mite, infested immunocompromised mice were provided ivermectin-compounded (12 ppm) feed without restriction for 8 wk (n = 10), were treated topically with moxidectin and imidacloprid (MI; 3 and 13 mg/kg, respectively) weekly for 8 wk (n = 10), or remained untreated (n = 10). Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159677PMC
September 2018
14 Reads

Sex- and Strain-related Differences in the Stress Response of Mice to CO₂ Euthanasia.

J Am Assoc Lab Anim Sci 2018 Sep 30;57(5):513-519. Epub 2018 Aug 30.

Comparative Medicine and Quality, The Jackson Laboratory, Bar Harbor, Maine.

The 2013 edition of the AVMA Guidelines for the Euthanasia of Animals recommends a 10% to 30% volume displacement rate (VDR) per minute for CO2 euthanasia of rodents. Here we sought to evaluate behavior and plasma catecholamine levels in multiple strains of male and female mice, euthanized individually or in a group, with CO2 VDR of 10% to 100%. Behavioral observations included ataxia, labored breathing, time to recumbency, time to surgical plane of anesthesia, and the number of jumps or paws at the face during the euthanasia process. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159681PMC
September 2018
10 Reads

DNA-based Determination of Ancestry in Cynomolgus Macaques ().

J Am Assoc Lab Anim Sci 2018 Sep 30;57(5):432-442. Epub 2018 Aug 30.

California National Primate Research Center, University of California, Davis, California, School of Mathematics and Natural Sciences, Arizona State University at the West Campus, Glendale, Arizona;, Email:

Interest in the genetic composition of cynomolgus macaques (Macaca fascicularis) has increased due to the rising demand for NHP models in human biomedical research. Significant genetic differences among regional populations of cynomolgus macaques can confound interpretations of research results because they do not solely reflect differences in experimental treatment effects. Therefore, the common origin of cynomolgus macaques used as research subjects should be verified by using region-specific genetic markers to minimize the influence of underlying genetic variation among animals selected as research subjects on phenotypes under study. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159685PMC
September 2018
29 Reads

Administering Fixed Oral Doses of Curcumin to Rats through Voluntary Consumption.

J Am Assoc Lab Anim Sci 2018 Sep 29;57(5):508-512. Epub 2018 Aug 29.

Department of Anatomical Pathology, Flinders University, Flinders Medical Centre, Adelaide, South Australia.

Curcumin, a polyphenol derived from turmeric, has a wide variety of therapeutic benefits including antiinflammatory, antioxidative, and chemopreventative effects. Oral gavage is widely performed to administer curcumin in laboratory rodents in several experimental models. Although effective, this method can increase stress in the animal, potentially influencing experimental results. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159675PMC
September 2018
17 Reads

Contaminated Shipping Materials Identified as the Source of Rotaviral Infection of Exported Mice.

J Am Assoc Lab Anim Sci 2018 Sep 14;57(5):529-533. Epub 2018 Aug 14.

Laboratory Animal Resource Center, University of California, San Francisco, California.

Over a 4-wk period in 2017, we received notification from 7 different institutions that mice exported from our SPF barrier facilities had tested positive for mouse rotavirus (MRV). The exports originated from several different buildings across multiple campuses. Our institution excludes MRV in all of our barrier facilities and has historically been free of this virus. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159673PMC
September 2018
1 Read

PCR Testing of Filter Material from IVC Lids for Microbial Monitoring of Mouse Colonies.

J Am Assoc Lab Anim Sci 2018 Sep 9;57(5):477-482. Epub 2018 Aug 9.

Georgetown University, Division of Comparative Medicine, Washington, DC;, Email:

Testing sentinel animals exposed to soiled bedding from colony animals is the most common method used for health monitoring in rodent facilities. Although environmental sampling is being explored-and, in many cases, has been implemented-as an alternative, exhaust plenum sampling is not effective for all ventilated rack designs. This study evaluated PCR testing of filter paper from sentinel cages on ventilated racks. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159679PMC
September 2018
1 Read

Evaluation of Analgesic Efficacy of Meloxicam and 2 Formulations of Buprenorphine after Laparotomy in Female Sprague-Dawley Rats.

J Am Assoc Lab Anim Sci 2018 Sep 9;57(5):498-507. Epub 2018 Aug 9.

Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois.

Managing postoperative pain in rodents is an important part of any animal care and use program, and identifying an optimal analgesic plan for a surgical procedure is critical to providing for animal welfare. Opioids and NSAID are commonly used in rodents, but few studies have evaluated their efficacy in surgical models. The current study aimed to evaluate the therapeutic efficacy of clinically relevant doses of buprenorphine (2 formulations) or meloxicam used in combination with ketamine and xylazine anesthesia in a Sprague-Dawley rat ovariohysterectomy surgical model. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159676PMC
September 2018
1 Read

Improving the Patency of Jugular Vein Catheters in Sprague-Dawley Rats by Using an Antiseptic Nitrocellulose Coating.

J Am Assoc Lab Anim Sci 2018 Sep 3;57(5):520-528. Epub 2018 Aug 3.

Divisions of Clinical Pharmacology, Indiana University School of Medicine, Indianapolis, Indiana;, Email:

Preclinical studies in animals often require frequent blood sampling over prolonged periods. A preferred method in rats is the implantation of a polyurethane catheter into the jugular vein, with heparinized glycerol as a lock solution. However, analysis of various biologic compounds (for example, microRNA) precludes the use of heparin. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159680PMC
September 2018
1 Read

Vibration-induced Behavioral Responses and Response Threshold in Female C57BL/6 Mice.

J Am Assoc Lab Anim Sci 2018 Sep 30;57(5):447-455. Epub 2018 Jul 30.

Division of Laboratory Animal Resources, Duke University Medical Center, Durham, North Carolina;, Email:

Despite documented adverse effects, limits for rodent exposure to vibration in the laboratory animal facility have not been established. This study used female C57BL/6 mice to determine the frequencies of vibration at which mice were most sensitive to behavioral changes, the highest magnitude of vibration that would not cause behavioral changes, the behavioral changes that occur in response to vibration, and the extent to which mice habituate to vibration. Mice were exposed to frequencies of vibration between 20 and 190 Hz at accelerations of 0. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-00092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159678PMC
September 2018
1 Read

Effect of Continuous Trio Breeding Compared with Continuous Pair Breeding in 'Shoebox' Caging on Measures of Reproductive Performance in Estrogen Receptor Knockout Mice.

J Am Assoc Lab Anim Sci 2018 Jul;57(4):328-334

Departments of Comparative Medicine, Oregon Health and Science University, Portland, Oregon.

Some performance standards for continuous trio breeding in 'shoebox' cages for inbred stocks and outbred strains of mice challenge the minimum floor space recommendations in the 8th edition of the Guide for the Care and Use of Laboratory Animals. In our study, we evaluated whether continuous trio breeding could be successfully applied to a breeding colony of genetically engineered mice housed in shoebox cages with a floor area of 67.6 in2. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059215PMC
July 2018
2 Reads

The Race to Publish.

Authors:
Virginia Dawson

J Am Assoc Lab Anim Sci 2018 Jul;57(4):326-327

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059212PMC
July 2018
2 Reads

Hepatic Vitamin A Concentrations in Vervets () Supplemented with Carotenoids Derived from Oil Palm.

J Am Assoc Lab Anim Sci 2018 Sep 18;57(5):456-464. Epub 2018 Jul 18.

Noncommunicable Diseases Research Unit, South African Medical Research Council, Cape Town, South Africa, Division of Medical Physiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa.

Commonly used in biomedical research, vervets (Chlorocebus aethiops) are omnivorous but primarily meet their vitamin A requirements from provitamin A carotenoids. Hypervitaminosis A has occurred in vervets that consume feed high in preformed vitamin A. We investigated the vitamin A status of vervets supplemented daily with various antioxidants derived from palm oil. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159682PMC
September 2018
13 Reads

Effects of 3 Rodent Beddings on Biochemical Measures in Rats and Mice.

J Am Assoc Lab Anim Sci 2018 Sep 16;57(5):443-446. Epub 2018 Jul 16.

Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.

Components of bedding might interact with experimental treatments and affect the outcome of various experiments. Here we studied the biochemical effects of 3 rodent bedding materials that are commonly used in Egypt. Male and female rats and mice were assigned randomly into 4 single-sex and single-species groups (10 animals per group). Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159672PMC
September 2018
17 Reads
1.120 Impact Factor

Facility-wide Eradication of by using PCR-validated Vaporized Hydrogen Peroxide.

J Am Assoc Lab Anim Sci 2018 Sep 13;57(5):465-476. Epub 2018 Jul 13.

Comparative Medicine, H. Lee Cancer Center & Research Institute, University of South Florida, Tampa, Florida.

Facility-wide Corynebacterium bovis eradication was established using vaporized hydrogen peroxide (VHP) decontamination guided by C. bovis PCR surveillance. Prior attempts limited to culling PCR-positive mice and decontaminating affected rooms were ineffective in preventing recurrence. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-17-000135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159683PMC
September 2018
32 Reads