3,264 results match your criteria Journal of proteomics[Journal]


Understanding the transition from water to land: Insights from multi-omic analyses of the perivitelline fluid of apple snail eggs.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, Hong Kong Baptist University, Hong Kong, China; HKBU Institute of Research and Continuing Education, Shenzhen, China. Electronic address:

Most gastropods deposit their eggs either on land or underwater, but the family Ampullariidae includes members that exhibit both underwater and aerial oviposition, making it an ideal model for understanding mechanisms underlying the evolutionary transition from water to land. We applied SDS-PAGE and LC-MS/MS to analyse the proteome of the egg perivitelline fluid (PVF) of Marisa cornuarietis - an aquatic ovipositing ampullariid. Comparison with the reported PVF proteomes of two aerial ovipositing ampullariids (Pomacea canaliculata and P. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183044
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http://dx.doi.org/10.1016/j.jprot.2018.12.014DOI Listing
December 2018
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Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University - New Jersey Medical School and Cancer Institute of New Jersey, Newark, NJ 07103, USA. Electronic address:

Disulfide bonds (SS) are post-translational modifications important for the proper folding and stabilization of many cellular proteins with therapeutic uses, including antibodies and other biologics. With budding advances of biologics and biosimilars, there is a mounting need for a robust method for accurate identification of SS. Even though several mass spectrometry methods have emerged for this task, their practical use rests on the broad effectiveness of both sample preparation methods and bioinformatics tools. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.010DOI Listing
December 2018

Identification of the proteoforms of surface localized Rod A of Aspergillus flavus and determination of the mechanism of proteoform generation.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Proteomics, Aravind Medical Research Foundation, Aravind Eye Care System, Dr. G. Venkataswamy Eye Research Institute, No:1 Anna Nagar, Madurai, TN, India. Electronic address:

Fungal keratitis is a serious, potentially sight-threatening corneal infection that is more prevalent in the tropical parts of the world including India, and A. flavus and Fusarium solani are the predominant etiological agents. The surface of fungal conidia is covered by hydrophobin family proteins, effectively masking the conidial antigens from immune cells. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.016DOI Listing
December 2018

Dissecting the molecular effects of cigarette smoke on proteasome function.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany. Electronic address:

Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke, one of the main risk factors for COPD, impairs proteasome function in vitro and in vivo. In the present study, we dissected the molecular changes induced by cigarette smoke on the proteasome in lung epithelial cells and mouse lungs. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.015DOI Listing
December 2018

Proteomics turns functional.

J Proteomics 2018 Dec 13. Epub 2018 Dec 13.

Department of Science and High Technology, Center of Bioinformatics, University of Insubria, Busto Arsizio, Italy. Electronic address:

Proteomics is acquiring a pivotal role in the comprehensive understanding of human biology. Biochemical processes involved in complex diseases, such as neurodegenerative diseases, diabetes and cancer, can be identified by combining proteomics analysis and bioinformatics tools. In the last ten years, the main output of differential proteomics investigations evolved from long lists of proteins to the generation of new hypotheses and their functional verification. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.012DOI Listing
December 2018

Response of the biomining Acidithiobacillus ferrooxidans to high cadmium concentrations.

J Proteomics 2018 Dec 13. Epub 2018 Dec 13.

Laboratory of Molecular Microbiology and Biotechnology, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile. Electronic address:

Cadmium is a heavy metal present in contaminated soils. It has no biological role but when entering cells generates DNA damage, overexpression of stress response proteins and misfolded proteins, amongst other deleterious effects. Acidithiobacillus ferrooxidans is an acidophilic bacterium resisting high concentrations of heavy metals such as cadmium. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.013DOI Listing
December 2018

Corrigendum to "Comparative analysis of novel autoantibody isotypes against citrullinatedinter-alpha-trypsin inhibitor heavy chain 3 (ITIH3) 542-556 peptide in serum from Taiwanese females with rheumatoid arthritis, primary Sjögren's syndrome and secondary Sjögren's syndrome in rheumatoid arthritis" [J Proteomics 141: (2016) 1-11].

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan; PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei 11031, Taiwan. Electronic address:

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http://dx.doi.org/10.1016/j.jprot.2018.11.016DOI Listing
December 2018

Corrigendum to "Isotypes of autoantibodies against differentially expressed novel malondialdehyde-modifiedpeptide adducts in serum of Taiwanese women with rheumatoid arthritis" [J Proteomics 170: (2018) 141-150].

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Department of Biotechnology and Animal Science, National Ilan University, Ilan 26047, Taiwan; School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan. Electronic address:

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http://dx.doi.org/10.1016/j.jprot.2018.11.017DOI Listing
December 2018

A decade of Nucleic Acid Programmable Protein Arrays (NAPPA) availability: News, actors, progress, prospects and access.

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain; Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain. Electronic address:

Understanding the dynamic of the proteome is a critical challenge because it requires high sensitive methodologies in high-throughput formats in order to decipher its modifications and complexity. While molecular biology provides relevant information about cell physiology that may be reflected in post-translational changes, High-Throughput (HT) experimental proteomic techniques are essential to provide valuable functional information of the proteins, peptides and the interconnections between them. Hence, many methodological developments and innovations have been reported during the last decade. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.007DOI Listing
December 2018

Improved SILAC method for double labeling of bacterial proteome.

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with advantages such as reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quantification accuracy, limiting the application of SILAC in bacterial proteomics to auxotrophic bacteria or to single labeling with lysine. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183044
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http://dx.doi.org/10.1016/j.jprot.2018.12.011DOI Listing
December 2018
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Early response of C2C12 myotubes to a sub-cytotoxic dose of hemorrhagic metalloproteinase HF3 from Bothrops jararaca venom.

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, Brazil. Electronic address:

Manifestations of local tissue damage, such as hemorrhage and myonecrosis, are among the most dramatic effects of envenomation by viperid snakes. Snake venom metalloproteinases (SVMPs) of the P-III class are main players of the hemorrhagic effect due to their activities in promoting blood vessel disruption. Hemorrhagic Factor 3 (HF3), a P-III class SVMP from Bothrops jararaca, shows a minimum hemorrhagic dose of 240 fmol on rabbit skin. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.006DOI Listing
December 2018
2 Reads

Effects of hypoxia-reoxygenation stress on mitochondrial proteome and bioenergetics of the hypoxia-tolerant marine bivalve Crassostrea gigas.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Department of Marine Biology, University of Rostock, Rostock, Germany; Department of Maritime Systems, Interdisciplinary Faculty, University of Rostock, Rostock, Germany.

Mitochondria are key intracellular targets of hypoxia-reoxygenation (H/R) stress due to their central role in generation of ATP and reactive oxygen species (ROS). Intertidal oysters Crassostrea gigas are adapted to frequent H/R cycles and maintain aerobic function despite frequent oxygen fluctuations. To gain insight into the molecular mechanisms of H/R tolerance, we assessed changes in mitochondrial respiration and (phospho)proteome of C. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.009DOI Listing
December 2018

Early-life exposure to the endocrine disruptor 17-α-ethinylestradiol induces delayed effects in adult brain, liver and ovotestis proteomes of a self-fertilizing fish.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Evolutionary and Adaptive Physiology - Institute of Life, Earth and Environment - University of Namur, 61 Rue de Bruxelles, B5000 Namur, Belgium.

Early-life represents a critically sensitive window to endocrine disrupting chemicals, potentially leading to long-term repercussions on the phenotype later in life. The mechanisms underlying this phenomenon, referred to as the Developmental Origins of Health and Disease (DOHaD), are still poorly understood. To gain molecular understanding of these effects, we exposed mangrove rivulus (Kryptolebias marmoratus) for 28 days post hatching (dph) to 4 and 120 ng/L 17-α-ethinylestradiol, a model xenoestrogen. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.008DOI Listing
December 2018

Ecotoxicoproteomics: A decade of progress in our understanding of anthropogenic impact on the environment.

J Proteomics 2018 Dec 6. Epub 2018 Dec 6.

Laboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, F-30207 Bagnols-sur-Cèze, France. Electronic address:

Anthropogenic pollutants are found worldwide. Their fate and effects on human and ecosystem health must be appropriately monitored. Today, ecotoxicology is focused on the development of new methods to assess the impact of pollutant toxicity on living organisms and ecosystems. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.001DOI Listing
December 2018
4 Reads

A proteomic approach to identify novel disease biomarkers in LCAT deficiency.

J Proteomics 2018 Dec 6. Epub 2018 Dec 6.

Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Italy. Electronic address:

Genetic LCAT deficiency is a rare recessive autosomal disease due to loss-of-function mutations in the gene coding for the enzyme lecithin:cholesterol acyltransferase (LCAT). Homozygous carriers are characterized by corneal opacity, haemolytic anaemia and renal disease, which represent the first cause of morbidity and mortality in these subjects. Diagnostic and prognostic markers capable of early detecting declining kidney function in these subjects are not available, and the specific serum or urine proteomic signature of LCAT deficient carriers has never been assessed. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.005DOI Listing
December 2018

Statistical analysis of proteomics data: A review on feature selection.

J Proteomics 2018 Dec 6. Epub 2018 Dec 6.

Department of Science and High Technology (DiSAT), University of Insubria, Busto Arsizio, Italy. Electronic address:

The spread of "-omics" strategies has strongly changed the way of thinking about the scientific method. Indeed, managing huge amounts of data imposes the replacement of the classical deductive approach with a data-driven inductive approach, so to generate mechanistical hypotheses from data. Data reduction is a crucial step in the process of proteomics data analysis, because of the sparsity of significant features in big datasets. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.004DOI Listing
December 2018

Postmortem protein stability investigations of the human hepatic drug-metabolizing cytochrome P450 enzymes CYP1A2 and CYP3A4 using mass spectrometry.

J Proteomics 2018 Dec 7. Epub 2018 Dec 7.

Department of Forensic Medicine, Aarhus University, Denmark.

Variability in expression and activity of hepatic drug-metabolizing cytochrome P450 (CYP) enzymes can play a causal role in fatal intoxication cases and is thus of forensic interest. We investigated the feasibility of LC-MS/MS based quantification and in vitro enzyme activity measurements of two major drug-metabolizing enzymes CYP1A2 and CYP3A4 in postmortem human liver microsomes (HLM). In autopsy cases (postmortem interval 24-36 h) we found CYP1A2 and CYP3A4 protein levels similar to that measured in a non-decayed reference HLM pool, whereas CYP1A2 and CYP3A4 enzyme activities were absent or severely decreased. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.024DOI Listing
December 2018

Proteomic analysis of protein homeostasis and aggregation.

J Proteomics 2018 Dec 6. Epub 2018 Dec 6.

Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Wita Stwosza 59, Gdansk 80308, Poland. Electronic address:

Protein homeostasis (proteostasis) refers to the ability of cells to preserve the correct balance between protein synthesis, folding and degradation. Proteostasis is essential for optimal cell growth and survival under stressful conditions. Various extracellular and intracellular stresses including heat shock, oxidative stress, proteasome malfunction, mutations and aging-related modifications can result in disturbed proteostasis manifested by enhanced misfolding and aggregation of proteins. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.003DOI Listing
December 2018

Quantifying proteins at microgram levels integrating gel electrophoresis and smartphone technology.

J Proteomics 2018 Dec 7. Epub 2018 Dec 7.

Spectrometry, Sample Preparation and Mechanization Group - GEPAM, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil; National Institute of Science and Technology for Bioanalytics, Brazil. Electronic address:

A combination between gel electrophoresis and smartphone technology is applied for quantifying proteins in a serum sample. Electrophoresis not only allows one to separate different proteins but also to build-up a calibration curve for those proteins. To expand its applicability, a smartphone allows one to capture a gel image, through its camera. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.002DOI Listing
December 2018

Proteomic analysis of olfactory bulb suggests CACNA1E as a promoter of CREB signaling in microbiota-induced depression.

J Proteomics 2018 Dec 4. Epub 2018 Dec 4.

Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Electronic address:

Major depressive disorders impact approximately 17% of the population worldwide, whose high morbidity and considerable adversity have resulted in enormous social and economic burden. In addition, clinically depressed patients often show reduced volume of olfactory bulb (OB) and decreased olfactory sensitivity. Although mounting evidence conveyed that the gut microbiota may implicate the pathophysiology of major depressive disorder (MDD) via the microbe-gut-brain axis, knowledge about its distinctive molecular mechanism is rudimentary. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.023DOI Listing
December 2018
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Dirhodium (II) complex interferes with iron-transport system to exert antibacterial action against Streptococcus pneumoniae.

J Proteomics 2018 Dec 4. Epub 2018 Dec 4.

Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

Drug resistance in bacteria is becoming a significant threat to global public health, and the development of novel and efficient antibacterial compounds is urgently needed. Recently, rhodium complexes have attracted attention as antimicrobial agents, yet their antibacterial mechanism remains unknown. In this study, we observed that the dirhodium (II) complex RhAc inhibited Streptococcus. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.021DOI Listing
December 2018
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An integrated quantitative proteomic and metabolomics approach to reveal the negative regulation mechanism of LamB in antibiotics resistance.

J Proteomics 2018 Dec 4. Epub 2018 Dec 4.

Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (School of Life Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, PR China. Electronic address:

Previously, a maltose-specific channel porin, LamB was found to be associate with multi-drug resistance in a lamB deleted strain, but the exact mechanisms require further elucidation. Herein, differential protein expression between the Escherichia coli mutant strain ΔlamB and the wild type strain BW25113, with and without ciprofloxacin (CFLX), was identified using iTRAQ based liquid chromatography-tandem mass spectrometry (LC-MS/MS); while differential metabolite expression was examined using gas chromatography-mass spectrometry (GC-MS). Further Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that lamB deletion leads to a decrease in several key metabolic pathways such as tricarboxylic acid (TCA) cycle, pentose phosphate pathway and glycolysis/gluconeogenesis. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.022DOI Listing
December 2018
3 Reads

Oncolytic adenovirus Delta-24-RGD induces a widespread glioma proteotype remodeling during autophagy.

J Proteomics 2018 Nov 29. Epub 2018 Nov 29.

Clinical Neuroproteomics Group, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain; IDISNA, Navarra Institute for Health Research, Pamplona, Spain; Proteored-ISCIII, Proteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain. Electronic address:

Adenovirus Delta-24-RGD has shown a remarkable efficacy in a phase I clinical trial for glioblastoma. Delta-24-RGD induces autophagy in glioma cells, however, the molecular derangements associated with Delta-24-RGD infection remains poorly understood. Here, proteomics was applied to characterize the glioma metabolic disturbances soon after Delta-24-RGD internalization and late in infection. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.020DOI Listing
November 2018
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Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy.

J Proteomics 2018 Nov 29. Epub 2018 Nov 29.

Laboratório de Biologia Celular, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil. Electronic address:

Chagas disease, caused by the protozoan Trypanosoma cruzi, affects millions of people worldwide, especially in Latin America. Approximately 30% of the cases evolve to the chronic symptomatic stage due to cardiac and/or digestive damage, generally accompanied by nervous system impairment. Given the higher frequency and severity of clinical manifestations related to cardiac tissue lesion, the goal of this study was the identification of proteins associated with the disease progression towards its cardiac form. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.019DOI Listing
November 2018
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Comparative proteomics of Tobacco mosaic virus-infected Nicotiana tabacum plants identified major host proteins involved in photosystems and plant defence.

J Proteomics 2018 Nov 29. Epub 2018 Nov 29.

Department of Biological Sciences, National University of Singapore (NUS), 14 Science Drive 4, 117543, Singapore; Temasek Life Sciences Laboratory, 1 Research Link, 117604, Singapore; National University of Singapore Suzhou Research Institute, Suzhou, Jiangsu 215123, China. Electronic address:

Tobacco mosaic virus (TMV) is a positive single-stranded RNA virus. Its 5' end ORF codes for the replicase proteins, namely 126 kDa and 183 kDa, respectively. These proteins interact with many host proteins to form a virus replication complex (VRC). Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.018DOI Listing
November 2018
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3.888 Impact Factor

Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate.

J Proteomics 2018 Nov 22. Epub 2018 Nov 22.

Research group of Host Microbe interaction, Department of Medical Biology, UiT- The Arctic University of Norway, Tromsø, Norway.

Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.013DOI Listing
November 2018

A resistant cowpea (Vigna unguiculata [L.] Walp.) genotype became susceptible to cowpea severe mosaic virus (CPSMV) after exposure to salt stress.

J Proteomics 2018 Nov 22. Epub 2018 Nov 22.

Department of Biochemistry and Molecular Biology, Federal University of Ceara, CE 60440-900, Brazil.

In nature, plants are simultaneously challenged by biotic and abiotic stresses. However, little is known about the effects of these combined stresses for most crops. This work aimed to evaluate the responsed of the virus-resistant cowpea genotype BRS-Marataoã to the exposure of salt stress combined with CPSMV infection. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.015DOI Listing
November 2018
1 Read
3.888 Impact Factor

Collagen sequence analysis of fossil camels, Camelops and c.f. Paracamelus, from the Arctic and sub-Arctic of Plio-Pleistocene North America.

J Proteomics 2018 Nov 22. Epub 2018 Nov 22.

Department of Biology & Department of Earth Sciences, Carleton University, 1125 Colonel By Dr, Ottawa, ON K1S 5B6, Canada.

Proteomic analyses of ancient remains are increasing in number and offer great potential to recover phylogenetic information on extinct animals beyond the reach of ancient DNA, but limitations in proteomic techniques remain unclear. Here we carry out LC-MS/MS sequence analysis of a ~3.5 million year old giant camel specimen from Nunavut along with the younger Pleistocene remains of the Yukon giant camel (c. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.014DOI Listing
November 2018
6 Reads

Proteomic analysis highlights the immune responses of the hepatopancreas against Hematodinium infection in Portunus trituberculatus.

J Proteomics 2018 Nov 20. Epub 2018 Nov 20.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; University of Chinese Academy of Sciences, Beijing 100049, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address:

The parasitic dinoflagellate Hematodinium is considered an important pathogen of economically important marine crustaceans and has been reported from many wild and cultured species. While limited studies have been conducted to reveal the host-parasite interaction in crustaceans, the underlying molecular mechanisms between Hematodinium and its crustacean hosts are scarcely known. We conducted a comprehensive study to investigate the proteomic responses to Hematodinium infection in the hepatopancreas of Portunus trituberculatus using an iTRAQ-based quantitative proteomic technology. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.012DOI Listing
November 2018
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Temporal proteomics of Arabidopsis plasma membrane during cold- and de-acclimation.

J Proteomics 2018 Nov 14. Epub 2018 Nov 14.

Department of Plant-bioscience, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan; United Graduate School of Agricultural Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan. Electronic address:

Freezing stress is one of the most important limiting factors of plant survival. Plants have developed a freezing adaptation mechanism upon sensing low temperatures (cold acclimation). Compositional changes in the plasma membrane, one of the initial sites of freezing injury, is prerequisite of achieving cold acclimation and have been investigated in several plant species. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183039
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http://dx.doi.org/10.1016/j.jprot.2018.11.008DOI Listing
November 2018
12 Reads

Using proteins to study how microbes contribute to soil ecosystem services: The current state and future perspectives of soil metaproteomics.

J Proteomics 2018 Nov 13. Epub 2018 Nov 13.

CEBAS-CSIC, Campus Universitario de Espinardo, Murcia E-30100, Spain.

Metaproteomics was established to analyse both the structure and the function of microbial communities and, particularly in soils, their contribution to ecosystem services. In this review, we provide an overview on how the study of the soil metaproteome can provide fundamental information on the role of microbial communities in soil ecosystem services. We further discuss the strengths and weaknesses of soil metaproteomics in comparison to other culture-independent OMIC techniques. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183040
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http://dx.doi.org/10.1016/j.jprot.2018.11.011DOI Listing
November 2018
4 Reads

A reflection on the role of ICP-MS in proteomics: Update and future perspective.

J Proteomics 2018 Nov 13. Epub 2018 Nov 13.

Department of Physical and Analytical Chemistry, University of Oviedo, Spain. Electronic address:

Mass spectrometry is the technique of reference for the identification and quantification of proteins. Whereas ESI and MALDI ionization sources are inherently not quantitative being highly influenced by the chemical nature of the analyte and the matrix, ICP-MS uses a hard ionization source that destroys proteins into its atoms and measures the elemental signal, which is independent of its chemical environment. As a consequence, ICP-MS turns up as an excellent technique for the screening, mapping and quantification of peptides and proteins in a sample through elemental detection (any element but C, H, N, or O) once they have been previously separated by chromatography. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183040
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http://dx.doi.org/10.1016/j.jprot.2018.11.010DOI Listing
November 2018
3 Reads

High sensitivity proteomics of prostate cancer tissue microarrays to discriminate between healthy and cancerous tissue.

J Proteomics 2018 Nov 12. Epub 2018 Nov 12.

MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, H-1117 Budapest, Hungary.

Biopsies, in the form of tissue microarrays (TMAs) were studied to identify anomalies indicative of prostate cancer at the proteome level. TMAs offer a valuable source of well-characterized biological material. However, because of the small tissue sample size method development was essential to provide the sensitivity and reliability necessary for the analysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183039
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http://dx.doi.org/10.1016/j.jprot.2018.11.009DOI Listing
November 2018
4 Reads

The C-type lectin-like receptor Nkrp1b: Structural proteomics reveals features affecting protein conformation and interactions.

J Proteomics 2018 Nov 8. Epub 2018 Nov 8.

BIOCEV- Institute of Microbiology of the Czech Academy of Sciences, Vestec, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic. Electronic address:

The cytotoxicity of mouse natural killer (NK) cells in response to pathological changes in target cells is regulated via the Nkrp1b receptor. Here, we characterized the Nkrp1b structure and structural features (stalk, loop, and oligomerization state) that affect its interactions. To study the Nkrp1b protein structure and the functional importance of its stalk, two Nkrp1b protein variants differing by the presence of the stalk were prepared. Read More

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https://www.sciencedirect.com/journal/journal-of-proteomics/
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https://linkinghub.elsevier.com/retrieve/pii/S18743919183039
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http://dx.doi.org/10.1016/j.jprot.2018.11.007DOI Listing
November 2018
6 Reads

Proteome characterization in various biological fluids of Trypanosoma brucei gambiense-infected subjects.

J Proteomics 2018 Nov 7. Epub 2018 Nov 7.

Institute of Neuroepidemiology and Tropical Neurology, School of Medicine, CNRS FR 3503 GEIST, University of Limoges, INSERM UMR 1094 Tropical Neuroepidemiology, Limoges, France. Electronic address:

Human African trypanosomiasis (HAT) is a neglected tropical disease that is endemic in sub-Saharan Africa. Control of the disease has been recently improved by better screening and treatment strategies, and the disease is on the WHO list of possible elimination. However, some physiopathological aspects of the disease transmission and progression remain unclear. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.005DOI Listing
November 2018
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Proteomics and Penicillium chrysogenum: Unveiling the secrets behind penicillin production.

J Proteomics 2018 Nov 7. Epub 2018 Nov 7.

INBIOTEC (Instituto de Biotecnología de León), Avda. Real 1 - Parque Científico de León, 24006 León, Spain; Departamento de Ciencias Biomédicas, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.

Discovery, industrial production and clinical applications of penicillin, together with scientific findings on penicillin biosynthesis and its complex regulation, are model milestones of the historical evolution of the most recognized 'magic bullet' against microbial infections available in the worldwide market. Thousands of tons of penicillin produced nowadays are the result of a huge number of technical, industrial and scientific tackled and solved challenges. This combination of, sometimes unsuspected, findings has given Proteomics the chance to support the understanding of the physiology of the high-producing fungal strains and the development of enhanced mutants by means of inverse engineering. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.006DOI Listing
November 2018

Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics.

J Proteomics 2018 Nov 7. Epub 2018 Nov 7.

State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, China; Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address:

Phosphorus (P) is a key macronutrient limiting cell growth and bloom formation of marine dinoflagellates. Physiological responses to changing ambient P have been investigated in dinoflagellates; however, the molecular mechanisms behind these responses remain limited. Here, we compared the protein expression profiles of a marine dinoflagellate Alexandrium catenella grown in inorganic P-replete, P-deficient, and inorganic- and organic-P resupplied conditions using an iTRAQ-based quantitative proteomic approach. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.004DOI Listing
November 2018
12 Reads

Manganese alters expression of proteins involved in the oxidative stress of Meyerozyma guilliermondii.

J Proteomics 2018 Nov 7. Epub 2018 Nov 7.

Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológica (NUPEB), Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Minas Gerais, Brazil. Electronic address:

Organisms, in general, respond to environmental stress by altering their pattern of protein expression (proteome), as an alternative to growing in stressful conditions. A strain of Meyerozyma guilliermondii resistant to manganese was isolated from a sample of water collected from mine drainage in southeastern Minas Gerais (Brazil), and demonstrated manganese detoxification capacity. Protein extracts containing the soluble fractions were obtained after growth of the strain in the absence and presence of MnSO. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.001DOI Listing
November 2018
4 Reads

Toward characterizing germination and early growth in the non-orthodox forest tree species Quercus ilex through complementary gel and gel-free proteomic analysis of embryo and seedlings.

J Proteomics 2018 Nov 6. Epub 2018 Nov 6.

Agroforestry and Plant Biochemistry, Proteomics, and Systems Biology Research Group, Department of Biochemistry and Molecular Biology- ETSIAM, University of Cordoba, UCO-CeiA3, Spain. Electronic address:

By using two complementary proteomics, gel-based and gel-free (shotgun) approaches, the protein profiles of the non-orthodox forest tree species Quercus ilex seeds during germination and early seedling growth have been compared. Proteins were extracted from embryo axis, radicle and shoot tissues at different developmental stages. Proteins were subjected to one- and two-dimensional gel electrophoresis. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.003DOI Listing
November 2018
3 Reads

Determination of food allergens by LC-MS: Impacts of sample preparation, food matrix, and thermal processing on peptide detectability and quantification.

J Proteomics 2018 Nov 5. Epub 2018 Nov 5.

Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry, University of Stuttgart, Allmandring 5b, Stuttgart 70569, Germany. Electronic address:

Food allergies are a growing worldwide concern and the contamination of products with food allergens represents a significant health risk to allergic consumers. With the introduction of reference doses, quantitative methods are needed for the monitoring of allergen levels, and the potential of LC-MS/MS is of hugely growing interest. In this study, we demonstrate that relevant food matrices (bakery products and chocolates) and thermal food processing substantially influence the quantification of 18 marker peptides from various nut and peanut allergens via targeted proteomics. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.11.002DOI Listing
November 2018
4 Reads

Integrated serum proteins and fatty acids analysis for putative biomarker discovery in inflammatory bowel disease.

J Proteomics 2018 Nov 2. Epub 2018 Nov 2.

Center for Translational Research on Autoimmune and Allergic Disease - CAAD, University of Piemonte Orientale, Novara, Italy; University of Turin, Medical School, Department of Public Health and Pediatric Sciences, Viral Pathogenesis Unit, Turin, Italy; University of Piemonte Orientale, Department of Translational Medicine, Virology Unit, Novara, Italy.

The diagnosis and management of Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is still challenging. There is no definitive gold standard diagnostic test, which is made on patient history and with endoscopic and histological findings. This study analyzed serum proteins and fatty acids using mass spectrometry-based techniques. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.017DOI Listing
November 2018
11 Reads

Venom proteome of Bungarus sindanus (Sind krait) from Pakistan and in vivo cross-neutralization of toxicity using an Indian polyvalent antivenom.

J Proteomics 2018 Oct 29. Epub 2018 Oct 29.

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Electronic address:

The proteome of the Pakistani B. sindanus venom was investigated with reverse-phase HPLC and nano-ESI-LCMS/MS analysis. At least 36 distinct proteins belonging to 8 toxin protein families were identified. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.016DOI Listing
October 2018
2 Reads
3.890 Impact Factor

Cytological and iTRAQ-based quantitative proteomic analyses of hau CMS in Brassica napus L.

J Proteomics 2018 Oct 29. Epub 2018 Oct 29.

National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:

Hau cytoplasmic male sterility (CMS) is a new type of CMS that was originally identified in Brassica juncea and subsequently transferred to B. napus and B. rapa. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.014DOI Listing
October 2018
4 Reads

Valorisation of the microalgae Nannochloropsis gaditana biomass by proteomic approach in the context of circular economy.

J Proteomics 2018 Oct 29. Epub 2018 Oct 29.

Research Project Manager of Innovation Department, Endesa Generación, S.A., Spain.

Nannochloropsis gaditana is a non-flagellated microalgae that has been widely used for different purposes, mostly related with the industrial production of biofuels or aquiculture. However, in order to increase the economic viability of the obtained microalgae biomass from a production plant coupled to a coal power plant, a proteomic approach was initiated by using fresh and atomized microalgae samples, as the main used commercial forms. Above 51,000 high quality spectra were obtained per sample in the MS/MS analysis of whole proteome of N. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.015DOI Listing
October 2018

Search for proteomic biomarkers related to bovine pre-slaughter stress using liquid isoelectric focusing (OFFGEL) and mass spectrometry.

J Proteomics 2018 Oct 29. Epub 2018 Oct 29.

Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46980 Paterna, Valencia, Spain. Electronic address:

Proteome changes derived from animals that have suffered pre-slaughter stress are a fact. In this study, Proteomic analysis was carried out on 20 bovine loin samples from Asturiana de los Valles and crossbreds cattle previously classified as normal and DFD meat at 24 h post-mortem using pH measurements. Sarcoplasmic sub-proteome of Longissimus thoracis at 24 h post-mortem was fractionated by the use of liquid isoelectric focusing (OFFGEL) in the pH range 3-10, followed by SDS-PAGE analysis of each retrieved fraction. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.013DOI Listing
October 2018

Identification and characterization of the zebra finch (Taeniopygia guttata) sperm proteome.

J Proteomics 2018 Oct 23. Epub 2018 Oct 23.

School of Life Sciences, Arizona State University, AZ, USA. Electronic address:

Spermatozoa exhibit remarkable variability in size, shape, and performance. Our understanding of the molecular basis of this variation, however, is limited, especially in avian taxa. The zebra finch (Taeniopygia guttata) is a model organism in the study of avian sperm biology and sperm competition. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.009DOI Listing
October 2018
10 Reads

Identification of nephropathy predictors in urine from children with a recent diagnosis of type 1 diabetes.

J Proteomics 2018 Oct 24. Epub 2018 Oct 24.

ProMiFa, Protein Microsequencing Facility, San Raffaele Scientific Institute, Milan, Italy. Electronic address:

Despite research progresses, the chance to accurately predict the risk for diabetic nephropathy (DN) is still poor. So far, the first evidence of DN is micro-albuminuria, which is detected only 10-20 years after the onset of diabetes. Our goal is to develop new predictive tools of nephropathy starting from urine, which can be easily obtained using noninvasive procedures and it is directly related to kidney. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.010DOI Listing
October 2018
5 Reads

Strategies for managing multi-patient 3D mass spectrometry imaging data.

J Proteomics 2018 Oct 19. Epub 2018 Oct 19.

The Maastricht Multimodal Molecular Imaging Institute (M4I), Maastricht University, 6229 ER Maastricht, the Netherlands. Electronic address:

Mass spectrometry imaging (MSI) has emerged as a powerful tool in biomedical research to reveal the localization of a broad scale of compounds ranging from metabolites to proteins in diseased tissues, such as malignant tumors. MSI is most commonly used for the two-dimensional imaging of tissues from multiple patients or for the three-dimensional (3D) imaging of tissue from a single patient. These applications are potentially introducing a sampling bias on a sample or patient level, respectively. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.008DOI Listing
October 2018
1 Read

Comparative proteomic analysis revealed complex responses to classical/novel duck reovirus infections in the spleen tissue of Cairna moschata.

J Proteomics 2018 Oct 16. Epub 2018 Oct 16.

Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address:

Duck reovirus (DRV), a member of the genus Orthoreovirus in the family Reoviridae, was first isolated from Muscovy ducks. The disease associated with DRV causes great economic losses to the duck industry. However, the responses of duck (Cairna moschata) to the classical/novel DRV (C/NDRV) infections are largely unknown. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.006DOI Listing
October 2018
8 Reads