3,308 results match your criteria Journal of proteomics[Journal]


Network analysis of DUSP12 partners in the nucleus under genotoxic stress.

J Proteomics 2019 Feb 16. Epub 2019 Feb 16.

Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil. Electronic address:

Dual Specificity Phosphatase 12 is a member of the Atypical DUSP Protein Tyrosine Phosphatase family, meaning that it does not contain typical MAP kinase targeting motifs, while being able to dephosphorylate tyrosine and serine/threonine residues. DUSP12 contains, apart from its catalytic domain, a zinc finger domain, making it one of the largest DUSPs, which displays strong nuclear expression in several tissues. In this work we identified nuclear targets of DUSP12 in two different cancer cell lines (A549 and MCF-7), challenging them with genotoxic stimuli to observe the effect on the networks and to link existing information about DUSP12 functions to the data obtained though mass spectrometry. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.008DOI Listing
February 2019

Optimisation of protein extraction for in-depth profiling of the cereal grain proteome.

J Proteomics 2019 Feb 15. Epub 2019 Feb 15.

CSIRO Agriculture and Food, 306 Carmody Rd, St Lucia, QLD 4067, Australia. Electronic address:

Cereal grain proteomics can provide valuable information regarding plant growth, nutritional status, adaptation to environmental stresses, and the role that grain proteins play in health disorders, such as coeliac disease. In this study liquid chromatography-mass spectrometry was used to compare the barley proteome after extraction using seven protocols, with and without defatting and protein precipitation steps that aimed to remove interfering secondary metabolites. Tris-HCl and urea buffers yielded 1405 and 1483 proteins (~79% overlap) from barley (cv Sloop). Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.009DOI Listing
February 2019

Quantitative label-free mass spectrometry using contralateral and adjacent breast tissues reveal differentially expressed proteins and their predicted impacts on pathways and cellular functions in breast cancer.

J Proteomics 2019 Feb 14. Epub 2019 Feb 14.

Genetics Department, Federal University of Parana, Curitiba, Brazil. Electronic address:

Proteins play an essential role in the biological processes associated with cancer. Their altered expression levels can deregulate critical cellular pathways and interactive networks. In this study, the mass spectrometry-based label-free quantification followed by functional annotation was performed to investigate the most significant deregulated proteins among tissues of primary breast tumor (PT) and axillary metastatic lymph node (LN) and corresponding non-tumor tissues contralateral (NCT) and adjacent (ANT) from patients diagnosed with invasive ductal carcinoma. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.007DOI Listing
February 2019
1 Read

Progress in farm animal proteomics: The contribution of combinatorial peptide ligand libraries.

J Proteomics 2019 Feb 11;197:1-13. Epub 2019 Feb 11.

Politecnico di Milano, Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Via Mancinelli 7, Milano 20131, Italy.

The present review covers proteomics discoveries in farm animals using combinatorial peptide ligand libraries (CPLLs). These libraries enhance the identification of low-abundance proteins by compressing the dynamic range of the protein concentration. This technology can be applied in multiple biological fluids, such as plasma or serum, follicular and cerebrospinal fluids, urine, saliva, tears seminal fluid, milk whey and even lymph. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.005DOI Listing
February 2019

Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey.

J Proteomics 2019 Feb 11. Epub 2019 Feb 11.

Technische Universität Berlin, Institut für Chemie, Strasse des 17. Juni 124, 10623 Berlin, Germany. Electronic address:

We report on the variable venom composition of a population of the Caucasus viper (Vipera kaznakovi) in Northeastern Turkey. We applied a combination of venom gland transcriptomics, and de-complexing bottom-up and top-down venomics. In contrast to sole bottom-up venomics approaches and gel or chromatography based venom comparison, our combined approach enables a faster and more detailed comparison of venom proteomes from multiple individuals. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.004DOI Listing
February 2019
1 Read

Integrative analyses of myocardial lipidome and proteome implicate mitochondrial dysfunction in lethal ventricular tachyarrhythmia (LVTA) induced by acute myocardial ischemia (AMI).

J Proteomics 2019 Feb 4;197:14-22. Epub 2019 Feb 4.

Department of Forensic Medicine, Central laboratory, Shantou University Medical College, Shantou, 15041, China.. Electronic address:

Lethal ventricular tachyarrhythmia (LVTA) is the most prevalent electrophysiological event leading to sudden cardiac death (SCD). In this study, the myocardial lipidome and proteome were analysed in rats experiencing LVTA as a consequence of acute myocardial ischemia (AMI). Results showed that 257 lipid species and 814 myocardial proteins were disrupted during LVTA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193003
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http://dx.doi.org/10.1016/j.jprot.2019.01.021DOI Listing
February 2019
5 Reads

Comparative proteomics and metabolomics of JAZ7-mediated drought tolerance in Arabidopsis.

J Proteomics 2019 Mar 5;196:81-91. Epub 2019 Feb 5.

Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China; Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology, University of Florida, Gainesville, FL, USA; Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, USA. Electronic address:

Jasmonates (JAs) are important phytohormones that regulate a wide range of plant processes, including growth, development and stress responses. Jasmonate ZIM-domain (JAZ) proteins are transcriptional repressors in JA signaling. Overexpression of JAZ7 was found to confer drought tolerance in Arabidopsis thaliana (A. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.001DOI Listing

iTRAQ-based analysis of leaf proteome identifies important proteins in secondary metabolite biosynthesis and defence pathways crucial to cross-protection against TMV.

J Proteomics 2019 Mar 3;196:42-56. Epub 2019 Feb 3.

Department of Biological Sciences, National University of Singapore (NUS), 14 Science Drive 4, Singapore 117543, Singapore; Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604, Singapore; National University of Singapore Suzhou Research Institute, Suzhou, Jiangsu 215123, China. Electronic address:

Cross-protection is a phenomenon in which infection with a mild virus strain protects host plants against subsequent infection with a closely related severe virus strain. This study showed that a mild strain mutant virus, Tobacco mosaic virus (TMV)-43A could cross protect Nicotiana benthamiana plants against wild-type TMV. Furthermore, we investigated the host responses at the proteome level to identify important host proteins involved in cross-protection. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.002DOI Listing
March 2019
2 Reads
3.888 Impact Factor

Mass spectrometry-based Shiga toxin identification: A clinical validation.

J Proteomics 2019 Feb 1. Epub 2019 Feb 1.

National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada; Department of Human Anatomy and Cell Sciences, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address:

After we published our preliminary study on the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and curated E. coli toxin databases on the identification of E. coli Shiga toxins (Stxs) in the Journal of Proteomics in year 2018, we were encouraged to further refine the method and test clinical isolates. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.020DOI Listing
February 2019

Proteome analysis of non-small cell lung cancer cell line secretomes and patient sputum reveals biofluid biomarker candidates for cisplatin response prediction.

J Proteomics 2019 Mar 30;196:106-119. Epub 2019 Jan 30.

Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Medical Oncology, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands. Electronic address:

Molecular markers are urgently needed to select non-small cell lung cancer (NSCLC) patients most likely to benefit from platinum-based chemotherapies. Of particular interest are proteins that can be found in biofluids like sputum for non-invasive detection. Therefore, we profiled the secretomes of 6 NSCLC cell lines with varying IC50-values for cisplatin, using label-free GeLC-MS/MS-based proteomics. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.018DOI Listing

Univariate and classification analysis reveals potential diagnostic biomarkers for early stage ovarian cancer Type 1 and Type 2.

J Proteomics 2019 Mar 30;196:57-68. Epub 2019 Jan 30.

Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden. Electronic address:

Biomarkers for early detection of ovarian tumors are urgently needed. Tumors of the ovary grow within cysts and most are benign. Surgical sampling is the only way to ensure accurate diagnosis, but often leads to morbidity and loss of female hormones. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.017DOI Listing
March 2019
2 Reads

Molecular pathways of varicocele and its repair - A paired labelled shotgun proteomics approach.

J Proteomics 2019 Mar 30;196:22-32. Epub 2019 Jan 30.

Department of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address:

Varicocelectomy is associated to improved semen quality and sperm functional quality, but individual response is highly variable. Thus, a prospective study was performed including 25 men who collected a semen sample before and 12 months after subinguinal microsurgical varicocelectomy. Semen analysis, sperm functional analysis, and seminal plasma proteomic analysis was performed before and 12 months after varicocelectomy, and according to improvement or not of semen quality (positive and negative outcome). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193002
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http://dx.doi.org/10.1016/j.jprot.2019.01.019DOI Listing
March 2019
2 Reads

On the characterisation of the porcine gland-specific salivary proteome.

J Proteomics 2019 Mar 29;196:92-105. Epub 2019 Jan 29.

Laboratory of Applied Veterinary Morphology, Department of Veterinary Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium; Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Electronic address:

To expand the knowledge on the porcine salivary proteome, secretions from the three major salivary glands were collected from anaesthetised piglets. Pilocarpine and isoproterenol were simultaneously injected intraperitoneally to increase the volume and protein concentration of the saliva, respectively. The protein composition and relative protein-specific abundance of saliva secreted by the parotid gland and by the mandibular and monostomatic sublingual gland, were determined using iTRAQ. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.016DOI Listing

Sensitive profiling of cell surface proteome by using an optimized biotinylation method.

J Proteomics 2019 Mar 29;196:33-41. Epub 2019 Jan 29.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China. Electronic address:

Cell surface proteins are responsible for many critical functions. Systematical profiling of these proteins would provide a unique molecular fingerprint to classify cells and provide important information to guide immunotherapy. Cell surface biotinylation method is one of the effective methods for cell surface proteome profiling. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.015DOI Listing

Platelet membrane lipid rafts protein composition varies following GPVI and CLEC-2 receptors activation.

J Proteomics 2019 Mar 22;195:88-97. Epub 2019 Jan 22.

Platelet Proteomics Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidad de Santiago de Compostela, Instituto de Investigación Sanitaria (IDIS), Santiago de Compostela, Spain. Electronic address:

Lipid rafts are membrane microdomains that have been proposed to play an important role in several platelet-signalling cascades, including those mediated by the receptors Glycoprotein VI (GPVI), and C-type lectin domain family 1 member B (CLEC-2), both involved in thrombus formation. We have performed a LC-MS/MS proteomic analysis of lipid rafts isolated from platelets activated through GPVI and CLEC-2 as well as from resting platelets. Our aim was to determine the magnitude of changes in lipid rafts protein composition and to elucidate the relevance of these alterations in platelet function. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.014DOI Listing
March 2019
4 Reads

Molecular characterization and in vitro interaction analysis of Op14-3-3 μ protein from Opuntia ficus-indica: identification of a new client protein from shikimate pathway.

J Proteomics 2019 Jan 22. Epub 2019 Jan 22.

IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí 78216, Mexico. Electronic address:

In plants, 14-3-3 proteins are important modulators of protein-protein interactions in response to environmental stresses. The aim of the present work was to characterize one Opuntia ficus-indica 14-3-3 and get information about its client proteins. To achieve this goal, O. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.013DOI Listing
January 2019
4 Reads

iTRAQ-based quantitative proteomics analysis of the spleen reveals innate immunity and cell death pathways associated with heat stress in broilers (Gallus gallus).

J Proteomics 2019 Mar 18;196:11-21. Epub 2019 Jan 18.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Heat stress induces immune dysfunction and cell death, but the mechanisms by which this occurs are not fully understood. Therefore, the isobaric tags for relative and absolute quantification (iTRAQ) was used to identify differentially abundant proteins (DAPs) in the spleen between heat-stressed group and control group, and real time qPCR (RT-qPCR), and Parallel Reaction Monitoring (PRM) were performed to validate the differentially abundant proteins of interest. The results showed that nine down regulated DAPs related to innate immunity were enriched in the Toll-like receptor signaling pathway (IRF3 and CD40), NOD-like receptor signaling pathway (TNFAIP3, IL-18, CathL2, IRF3, IAP3 and CYBA), RIG-I-like receptor signaling pathway (TRIM25 and IRF3), and Cytosolic DNA-sensing pathway (IL-18, POLR3F and IRF3). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193002
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http://dx.doi.org/10.1016/j.jprot.2019.01.012DOI Listing
March 2019
4 Reads

A large scale proteome analysis of the gefitinib primary resistance overcome by KDAC inhibition in KRAS mutated adenocarcinoma cells overexpressing amphiregulin.

J Proteomics 2019 Mar 17;195:114-124. Epub 2019 Jan 17.

Univ. Grenoble Alpes, LBFA and BEeSy, PROMETHEE Proteomic Platform, Grenoble, France; Inserm, U1055, PROMETHEE Proteomic Platform, Grenoble, France; CHU Grenoble Alpes, Institut de Biologie et de Pathologie, PROMETHEE Proteomic Platform, Grenoble, France. Electronic address:

KDAC inhibitors (KDACi) overcome gefitinib primary resistance in non-small cell lung cancer (NSCLC) including mutant-KRAS lung adenocarcinoma. To identify which proteins are involved in the restoration of this sensitivity and to provide new therapeutic targets for mutant-KRAS lung adenocarcinoma, we performed an iTRAQ quantitative proteomic analysis after subcellular fractionation of H358-NSCLC treated with gefitinib and KDACi (TSA/NAM) versus gefitinib alone. The 86 proteins found to have been significantly dysregulated between the two conditions, were mainly involved in cellular metabolism and cell transcription processes. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.009DOI Listing
March 2019
3 Reads

High-resolution quantitative proteomics applied to the study of the specific protein signature in the sputum and saliva of active tuberculosis patients and their infected and uninfected contacts.

J Proteomics 2019 Mar 18;195:41-52. Epub 2019 Jan 18.

Spanish National Research Council (CSIC), Vigo, Pontevedra, Spain. Electronic address:

Our goal was to establish panels of protein biomarkers that are characteristic of patients with microbiologically confirmed pulmonary tuberculosis (TB) and their contacts, including latent TB-infected (LTBI) and uninfected patients. Since the first pathogen-host contact occurs in the oral and nasal passages the saliva and sputum were chosen as the biological fluids to be studied. Quantitative shotgun proteomics was performed using a LTQ-Orbitrap-Elite platform. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.010DOI Listing
March 2019
10 Reads

Quantitative proteomics reveals systematic dysregulations of liver protein metabolism in sucralose-treated mice.

J Proteomics 2019 Mar 17;196:1-10. Epub 2019 Jan 17.

Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States. Electronic address:

Sucralose, one kind of "sugar-free" artificial sweeteners, is widely used as food and drinks additives. It is generally considered that sucralose is safe because majority of ingested sucralose is not metabolized and absorbed by human body. However, increasing evidence shows the negative effects of artificial sweeteners in perturbations of gut microbiota which plays an important role in a variety of processes related to host health such as immune system development. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193001
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http://dx.doi.org/10.1016/j.jprot.2019.01.011DOI Listing
March 2019
4 Reads
3.888 Impact Factor

Lable-free based comparative proteomic analysis of secretory proteins of rough Brucella mutants.

J Proteomics 2019 Mar 17;195:66-75. Epub 2019 Jan 17.

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, PR China. Electronic address:

Brucella rough mutants are reported to induce infected macrophage death, which is type IV secretion system (T4SS) dependent. T4SS and its secretory proteins play a major role in host-bacteria interactions, but the crucial secretory proteins to promote macrophage death during Brucella rough mutant infection have not been characterized. In this study, we found that T4SS components played no role for macrophage death induced by Brucella rough mutant infection, but some T4SS effectors did. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193001
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http://dx.doi.org/10.1016/j.jprot.2019.01.008DOI Listing
March 2019
7 Reads

First look into the venom of Roatan Island's critically endangered coral snake Micrurus ruatanus: Proteomic characterization, toxicity, immunorecognition and neutralization by an antivenom.

J Proteomics 2019 Jan 17. Epub 2019 Jan 17.

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address:

A proteomic and toxicological study of the venom from one specimen of Micrurus ruatanus, a critically endangered coral snake species endemic to Roatan Island, Honduras, was carried out. Immunorecognition and neutralization of venom lethality by an anticoral antivenom was also evaluated. Forty peaks were collected from RP-HPLC fractionation of the venom. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.007DOI Listing
January 2019
1 Read

Accumulation of virulence-associated proteins in Campylobacter jejuni Outer Membrane Vesicles at human body temperature.

J Proteomics 2019 Mar 11;195:33-40. Epub 2019 Jan 11.

Department of Molecular Biology, Umeå University, Umeå 90187, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå 90187, Sweden. Electronic address:

Campylobacter jejuni is the major cause of bacterial gastroenteritis in humans. In contrast, colonization in avian hosts is asymptomatic. Body temperature differs between human (37 °C) and avian (42 °C) hosts, and bacterial growth in 37 °C is therefore a potential cue for higher virulence properties during human infection. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.005DOI Listing
March 2019
1 Read

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

J Proteomics 2019 Mar 11;195:98-113. Epub 2019 Jan 11.

Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China; Changchun Sci-Tech University, Changchun 130600, China. Electronic address:

The ability to activate and regulate stem cells during wound healing and tissue/organ regeneration is a promising field which could bring innovative approaches to regenerative medicine. The regenerative capacity of invertebrates has been well documented, however in mammals, stem cells that drive organ regeneration are rare. Deer antler is unique in providing a mammalian model of complete organ regeneration based on stem cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193001
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http://dx.doi.org/10.1016/j.jprot.2019.01.004DOI Listing
March 2019
2 Reads

Increased growth rate and productivity following stable depletion of miR-7 in a mAb producing CHO cell line causes an increase in proteins associated with the Akt pathway and ribosome biogenesis.

J Proteomics 2019 Mar 11;195:23-32. Epub 2019 Jan 11.

National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.

Cell line engineering using microRNAs represents a desirable route for improving the efficiency of recombinant protein production by CHO cells. In this study we generated stable CHO DP12 cells expressing a miR-7 sponge transcript which sequesters miR-7 from its endogenous targets. Depletion of miR-7 results in a 65% increase in cell growth and >3-fold increase in yield of secreted IgG protein. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.003DOI Listing
March 2019
1 Read

Multivariate data analysis for the detection of human alpha-acid glycoprotein aberrant glycosylation in pancreatic ductal adenocarcinoma.

J Proteomics 2019 Mar 11;195:76-87. Epub 2019 Jan 11.

Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain.

Relative quantification of human alpha-acid glycoprotein (hAGP) glycan isomers using [C]/[C]-aniline in combination with multivariate data analysis is proposed as an efficient method for the identification of pancreatic ductal adenocarcinoma (PDAC) glycan biomarkers in serum samples. Intact and desialylated glycans from hAGP, purified from serum samples of patients with PDAC and chronic pancreatitis (ChrP), were labeled with aniline and analyzed by μZIC-HILIC-MS. Afterwards, partial least squares discriminant analysis (PLS-DA) was applied to the relative areas obtained for all glycan isomers in the different samples: pathological (ChrP or PDAC) versus healthy samples. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.01.006DOI Listing
March 2019
3 Reads

Proteomic characterization of outer membrane vesicles from gut mucosa-derived fusobacterium nucleatum.

J Proteomics 2019 Mar 8;195:125-137. Epub 2019 Jan 8.

Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States. Electronic address:

Fusobacterium nucleatum is a Gram-negative bacterium commonly found in the oral cavity and is often involved in periodontal diseases. Recent studies have shown increased F. nucleatum prevalence in colorectal cancer (CRC) tissues, and causal data has linked this bacterium to CRC tumorigenesis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183045
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http://dx.doi.org/10.1016/j.jprot.2018.12.029DOI Listing
March 2019
11 Reads

The protein S-nitrosylation of splicing and translational machinery in vascular endothelial cells is susceptible to oxidative stress induced by oxidized low-density lipoprotein.

J Proteomics 2019 Mar 7;195:11-22. Epub 2019 Jan 7.

National Glycoengineering Research Center, The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital, Shandong University, Jinan, Shandong 250021, China. Electronic address:

Oxidized low-density lipoprotein (ox-LDL) can impair endothelial function and lead to the atherosclerosis development. Protein S-nitrosylation is sensitive to cellular redox state and acts as a crucial regulator and executor of nitric oxide (NO) signaling pathways. Aberrant S-nitrosylation contributes to the pathogenesis of cardiovascular and cerebrovascular diseases. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193000
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http://dx.doi.org/10.1016/j.jprot.2019.01.001DOI Listing
March 2019
8 Reads

SWATH proteomic profiling of prostate cancer cells identifies NUSAP1 as a potential molecular target for Galiellalactone.

J Proteomics 2019 Feb 25;193:217-229. Epub 2018 Oct 25.

Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain; Hospital Universitario Reina Sofía, Madrid, Spain.

Galiellalactone (GL) is a fungal metabolite that presents antitumor and antiinflammatory activities in vitro and in vivo. Previous studies have shown that GL targets NF-κB and STAT3 pathways and induces G/M cell cycle arrest in androgen-insensitive prostate cancer cells. In this study, we show that GL-induced cell cycle arrest is independent of the NF-κB and STAT3 pathways in DU145 and PC-3 cells, and also that GL did not affect cell cycling in androgen-sensitive prostate cancer cells such as LNCaP and 22Rv1 cells. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.10.012DOI Listing
February 2019
1 Read

Plasma proteome profiling of high-altitude polycythemia using TMT-based quantitative proteomics approach.

J Proteomics 2019 Mar 31;194:60-69. Epub 2018 Dec 31.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China. Electronic address:

High-altitude polycythemia (HAPC) is one of the classic chronic mountain sicknesses and has been a serious public health problem in high-altitude regions. Despite numerous studies on HAPC via genomics or transcriptomics approaches, the pathogenesis of HAPC is still unclear. Here, we performed a TMT- based comparative quantitative proteomics analysis to reveal the changes of plasma proteomics profiles between HAPC subjects and healthy controls. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.031DOI Listing
March 2019
1 Read

SWATH based quantitative proteomics analysis reveals Hfq2 play an important role on pleiotropic physiological functions in Aeromonas hydrophila.

J Proteomics 2019 Mar 28;195:1-10. Epub 2018 Dec 28.

Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (School of Life Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, PR China. Electronic address:

The RNA-binding protein Hfq protein is a well-characterized post-transcriptional regulator and plays an important role in the regulation of various physiological functions. Most bacterial genomes have only one copy of hfq, but a few bacterial species carry another distinct copy of hfq (hfq2) on the chromosome. However, the physiological properties of Hfq2 remain elusive. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.030DOI Listing
March 2019
2 Reads
3.888 Impact Factor

iTRAQ-based quantitative proteomics reveals insights into metabolic and molecular responses of glucose-grown cells of Rubrivivax benzoatilyticus JA2.

J Proteomics 2019 Mar 28;194:49-59. Epub 2018 Dec 28.

Department of Plant Sciences, P.O. Central University, University of Hyderabad, Hyderabad 500046, India. Electronic address:

Anoxygenic photosynthetic bacteria thrive under diverse habitats utilising an extended range of inorganic/organic compounds under different growth modes. Although they display incredible metabolic flexibility, their responses and adaptations to changing carbon regimes is largely unexplored. In the present study, we employed iTRAQ-based global proteomic profiling and physiological studies to uncover the adaptive strategies of a phototrophic bacterium, Rubrivivax benzoatilyticus JA2 to glucose. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.027DOI Listing
March 2019
1 Read

Polyphosphate kinase 1 of Burkholderia pseudomallei controls quorum sensing, RpoS and host cell invasion.

J Proteomics 2019 Mar 28;194:14-24. Epub 2018 Dec 28.

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand; Center for Neuroscience, Faculty of Science, Mahidol University, Bangkok, Thailand. Electronic address:

Burkholderia pseudomallei is a Gram negative bacterium and the causative agent of melioidosis. Nonetheless, how virulence factors and pathogenic mechanisms are regulated have been elusive. In this study, we determined a role of polyphosphate kinase 1 (Ppk1) in regulation of quorum sensing (QS) and the sigma factor RpoS, and identified genes co-regulated by Ppk1, QS and RpoS. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.024DOI Listing
March 2019
1 Read

Exosome beads array for multiplexed phenotyping in cancer.

J Proteomics 2018 Dec 28. Epub 2018 Dec 28.

Department of Medicine and Cytometry General Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain; Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain.

Exosomes are small extracellular vesicles (EV) released from all cells that differ from others EV in their cellular origin, abundance and biogenesis. These different types of extracellular vesicles are recognized as potential markers of human diseases, including cancer and, in recent years, there has been an important advance in the molecular characterization of exosomes from different types of cancer. In particular, due to their presence and stability in most body fluids and the similarity of their content with tumor cells, exosomes have great potential as non-invasive biomarkers for liquid biopsy. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.023DOI Listing
December 2018
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Integrative hepatic metabolomics and proteomics reveal insights into the mechanism of different feed efficiency with high or low dietary forage levels in Holstein heifers.

J Proteomics 2019 Mar 28;194:1-13. Epub 2018 Dec 28.

State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, PR China. Electronic address:

Feed efficiency (FE) can be affected by dietary composition, management, and metabolic conditions. The liver is the central metabolic organ in cattle; however, specific metabolic changes in the liver under different dietary forage levels have not been clarified. Therefore, this study aimed to investigate the hepatic physiology mechanisms of high or low dietary forage levels (80% or 20% forage, namely S80 and S20) on FE in dairy heifers using integrative metabolomic and proteomic methods. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.026DOI Listing
March 2019
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Cone snail prolyl-4-hydroxylase α-subunit sequences derived from transcriptomic data and mass spectrometric analysis of variable proline hydroxylation in C. amadis venom.

J Proteomics 2019 Mar 27;194:37-48. Epub 2018 Dec 27.

Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India; National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India. Electronic address:

Putative prolyl-4-hydroxylase (P4H) α-subunit sequences have been extracted by mining transcriptomic data obtained from seven cone snail species C. amadis, C. monile, C. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.028DOI Listing

Silac-based quantitative proteomic analysis of Lactobacillus reuteri CRL 1101 response to the presence of selenite and selenium nanoparticles.

J Proteomics 2019 Mar 27;195:53-65. Epub 2018 Dec 27.

Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Av. Complutense s/n, 28040 Madrid, Spain. Electronic address:

Stable isotope labeling in cell culture (SILAC) was applied for the first time on a lactic acid bacterium strain (L. reuteri CRL1101) for analyzing differential protein expression associated to selenite(NaSeO) and selenium nanoparticles (SeNPs) exposure. 57 and 47 proteins were found de-regulated by >1,5 fold in presence of selenite and SeNPs, respectively. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.025DOI Listing

Differential protein expression of blood platelet components associated with adverse transfusion reactions.

J Proteomics 2019 Mar 24;194:25-36. Epub 2018 Dec 24.

GIMAP-EA3064, University of Lyon, Saint-Etienne, France; French Blood Bank (EFS) Auvergne-Rhône-Alpes, Saint-Etienne, France. Electronic address:

Platelets found within platelet components (PCs) intended for transfusion release inflammatory molecules. Despite the implementation of leukoreduction, some of these PCs are occasionally associated with adverse transfusion reactions (ATRs). The aim of this study was to decipher the platelet proteome in two types of PCs, buffy-coat-derived pooled PCs (PPCs) and single-donor apheresis PCs (SDA-PCs), associated with ATRs. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.019DOI Listing
March 2019
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Potential mechanism of nitrite degradation by Lactobacillus fermentum RC4 based on proteomic analysis.

J Proteomics 2019 Mar 21;194:70-78. Epub 2018 Dec 21.

Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China; Food Science and Nutrition Department, Nanjing Normal University, Nanjing, China. Electronic address:

Nitrite helps meat products retain colour and prevents decay, but excessive intake can be carcinogenic. To investigate the mechanism of nitrite degradation by lactic acid bacteria, Lactobacillus fermentum RC4 were grown in broth containing NaNO at 0, 100, 300, or 500 mg/L, and 8-plex iTRAQ proteomics and bioinformatics analysis were employed to explore protein expression patterns during nitrite degradation. Real-time PCR was used to confirm mRNA expression levels of selected genes. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.021DOI Listing
March 2019
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Chronic exposure of bumblebees to neonicotinoid imidacloprid suppresses the entire mevalonate pathway and fatty acid synthesis.

J Proteomics 2019 Mar 21;196:69-80. Epub 2018 Dec 21.

R&D Department, Biobest Group, Ilse Velden 18, Westerlo B-2260, Belgium.

Determining the side effects of pesticides on pollinators is an important topic due to the increasing loss of pollinators. We aimed to determine the effects of chronic sublethal exposure of the neonicotinoid pesticide imidacloprid on the bumblebee Bombus terrestris under laboratory conditions. The analytical standard of imidacloprid in sugar solution was used for the treatment. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.022DOI Listing
March 2019
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Affinity chromatography revealed 14-3-3 interactome of tomato (Solanum lycopersicum L.) during blue light-induced de-etiolation.

J Proteomics 2019 Feb 22;193:44-61. Epub 2018 Dec 22.

Department of Molecular Biology, Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University in Olomouc, Šlechtitelu 27, 783 71 Olomouc, Czechia. Electronic address:

De-etiolation is the first developmental process under light control allowing the heterotrophic seedling to become autotrophic. The phytohormones cytokinins (CKs) largely contribute to this process. Reversible phosphorylation is a key event of cell signaling, allowing proteins to become active or generating a binding site for specific protein interaction. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.017DOI Listing
February 2019

Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber Apostichopus japonicus.

J Proteomics 2019 Feb 21;193:27-43. Epub 2018 Dec 21.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address:

Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.020DOI Listing
February 2019
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Physiological and TMT-based proteomic analysis of oat early seedlings in response to alkali stress.

J Proteomics 2019 Feb 18;193:10-26. Epub 2018 Dec 18.

Science Innovation Team of Oats, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China.

Oats are an important cereal crop worldwide, and they also serve as a phytoremediation crop to ameliorate salinized and alkalized soils. However, the mechanism of the oat response to alkali remains unclear. Physiological and tandem mass tag (TMT)-based proteomic analyses were employed to elucidate the mechanism of the oat response to alkali stress. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.018DOI Listing
February 2019
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Understanding the transition from water to land: Insights from multi-omic analyses of the perivitelline fluid of apple snail eggs.

J Proteomics 2019 Mar 14;194:79-88. Epub 2018 Dec 14.

Department of Biology, Hong Kong Baptist University, Hong Kong, China; HKBU Institute of Research and Continuing Education, Shenzhen, China. Electronic address:

Unlike most of the freshwater gastropod families, the family Ampullariidae includes members that exhibit both underwater and aerial oviposition, making it an ideal model for understanding mechanisms underlying the evolutionary transition from water to land. We applied SDS-PAGE and LC-MS/MS to analyse the proteome of the egg perivitelline fluid (PVF) of Marisa cornuarietis - an aquatic ovipositing ampullariid. Comparison with the reported PVF proteomes of two aerial ovipositing ampullariids (Pomacea canaliculata and P. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.014DOI Listing
March 2019
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Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University - New Jersey Medical School and Cancer Institute of New Jersey, Newark, NJ 07103, USA. Electronic address:

Disulfide bonds (SS) are post-translational modifications important for the proper folding and stabilization of many cellular proteins with therapeutic uses, including antibodies and other biologics. With budding advances of biologics and biosimilars, there is a mounting need for a robust method for accurate identification of SS. Even though several mass spectrometry methods have emerged for this task, their practical use rests on the broad effectiveness of both sample preparation methods and bioinformatics tools. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.010DOI Listing
December 2018
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Identification of the proteoforms of surface localized Rod A of Aspergillus flavus and determination of the mechanism of proteoform generation.

J Proteomics 2019 Feb 15;193:62-70. Epub 2018 Dec 15.

Department of Proteomics, Aravind Medical Research Foundation, Aravind Eye Care System, Dr. G. Venkataswamy Eye Research Institute, No:1 Anna Nagar, Madurai, TN, India. Electronic address:

Fungal keratitis is a serious, potentially sight-threatening corneal infection that is more prevalent in the tropical parts of the world including India, and A. flavus and Fusarium solani are the predominant etiological agents. The surface of fungal conidia is covered by hydrophobin family proteins, effectively masking the conidial antigens from immune cells. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.016DOI Listing
February 2019
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Dissecting the molecular effects of cigarette smoke on proteasome function.

J Proteomics 2019 Feb 14;193:1-9. Epub 2018 Dec 14.

Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany. Electronic address:

Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke, one of the main risk factors for COPD, impairs proteasome function in vitro and in vivo. In the present study, we dissected the molecular changes induced by cigarette smoke on the proteasome in lung epithelial cells and mouse lungs. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.015DOI Listing
February 2019
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Proteomics turns functional.

J Proteomics 2018 Dec 13. Epub 2018 Dec 13.

Department of Science and High Technology, Center of Bioinformatics, University of Insubria, Busto Arsizio, Italy. Electronic address:

Proteomics is acquiring a pivotal role in the comprehensive understanding of human biology. Biochemical processes involved in complex diseases, such as neurodegenerative diseases, diabetes and cancer, can be identified by combining proteomics analysis and bioinformatics tools. In the last ten years, the main output of differential proteomics investigations evolved from long lists of proteins to the generation of new hypotheses and their functional verification. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.012DOI Listing
December 2018
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Response of the biomining Acidithiobacillus ferrooxidans to high cadmium concentrations.

J Proteomics 2018 Dec 13. Epub 2018 Dec 13.

Laboratory of Molecular Microbiology and Biotechnology, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile. Electronic address:

Cadmium is a heavy metal present in contaminated soils. It has no biological role but when entering cells generates DNA damage, overexpression of stress response proteins and misfolded proteins, amongst other deleterious effects. Acidithiobacillus ferrooxidans is an acidophilic bacterium resisting high concentrations of heavy metals such as cadmium. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.013DOI Listing
December 2018