380,233 results match your criteria Journal of pharmacy & bioallied sciences[Journal]


Verapamil prevents the effect of calcium-sensing receptor activation on the blood glucose and insulin levels in rats.

Pharmacol Rep 2019 Jan 25;71(3):478-484. Epub 2019 Jan 25.

Department of Pathophysiology, Faculty of Pharmacy, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

Background: The Ca triggered insulin exocytosis in β cells of the pancreatic islets may be the result of Ca influx through L-type voltage dependent calcium channels (VDCC) localized in the plasma membrane, as well as of liberation of Ca from intracellular storages, induced by activation of the calcium receptor (CaR) coupled with the PLC enzyme present in the pancreatic islets. The present study was designated to determine, in in vivo experiments, the effects of CaR activation by R-568 and inhibition of the receptor by NPS 2143 on the plasma glucose and insulin levels in the presence of verapamil, a calcium channel blocker.

Methods: Wistar rats, after fasting for 14 h before the experiment, were anesthetized with inactin and loaded ip with 1 g/kg glucose. Read More

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http://dx.doi.org/10.1016/j.pharep.2019.01.004DOI Listing
January 2019

Influence of salbutamol on the anticonvulsant potency of the antiepileptic drugs in the maximal electroshock-induced seizures in mice.

Pharmacol Rep 2019 Feb 8;71(3):466-472. Epub 2019 Feb 8.

Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland.

Background: β-Adrenergic receptor agonists are widely used agents in the treatment of asthma or preterm labor. Since prevalence of asthma was shown to be higher in patients with epilepsy and modulation of noradrenergic system activity may modify epilepsy course, the aim of the present study was to examine the effect of salbutamol (SALB), one of the most commonly used β-adrenergic receptor agonist on the anticonvulsant potency of four classical antiepileptic drugs (AEDs): valproate (VPA), carbamazepine (CBZ), phenytoin (DPH) and phenobarbital (PB) in mice subjected to the maximal electroshock (MES)-induced seizures.

Methods: Seizures were caused by a current delivered through ear-clip electrodes. Read More

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http://dx.doi.org/10.1016/j.pharep.2019.02.003DOI Listing
February 2019

Antifibrotic effects of Fraxetin on carbon tetrachloride-induced liver fibrosis by targeting NF-κB/IκBα, MAPKs and Bcl-2/Bax pathways.

Pharmacol Rep 2019 Jan 15;71(3):409-416. Epub 2019 Jan 15.

Department of Pharmacy, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Background: Liver fibrosis is a chronic lesion which ultimately results in cirrhosis and possible death. Although the high incidence and lethality, few therapies are effective for liver fibrosis. Fraxetin (7,8-dihydroxy-6-methoxy coumarin), a natural product extracted from cortex fraxini, has exhibited a significant hepatoprotective and anti-fibrotic properties. Read More

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http://dx.doi.org/10.1016/j.pharep.2019.01.008DOI Listing
January 2019

Facile synthesis of manganese oxide-embedded mesoporous carbons and their adsorbability towards methylene blue.

Chemosphere 2019 Apr 11;227:455-461. Epub 2019 Apr 11.

NTT Hi-Tech Institute, Nguyen Tat Thanh University, 298-300A Nguyen Tat Thanh, Ward 13, District 4, Ho Chi Minh City, 700000, Viet Nam. Electronic address:

Herein, a facile strategy to fabricate the novel manganese oxide-imprinted mesoporous carbons (MOPCx, x presents for pyrolysis temperature) was described via the direct pyrolysis of Mn(BDC)(DMF) (BDC = 1,4-benzenedicarboxylate, DMF = N,N-dimethylformamide) as a self-sacrificed template at various temperatures (x = 550, 750, and 950 C). The characterization results demonstrated the existence of MnO embedded in carbon structures with different morphologies, and enhancing surface areas (249.86-294. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.04.079DOI Listing

Removal of copper ions from aqueous solution using low temperature biochar derived from the pyrolysis of municipal solid waste.

Sci Total Environ 2019 Apr 10;673:777-789. Epub 2019 Apr 10.

College of Engineering, Design and Physical Sciences, Brunel University London, Uxbridge, Middlesex UB8 3PH, UK. Electronic address:

Sustainable methods to produce filter materials are needed to remove a variety of pollutants found in water including organic compounds, heavy metals, and other harmful inorganic and biological contaminants. This study focuses on the removal of Cu(II) from copper aqueous solutions using non-activated char derived from the pyrolysis of mixed municipal discarded materials (MMDM) using a new heat pipe-based pyrolysis reactor. Adsorption experiments were conducted by adding the char to copper solutions of varying concentration (50-250 mg/L) at a constant temperature of 30 °C. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.04.085DOI Listing

6-Methoxyflavonols from the aerial parts of Tetragonia tetragonoides (Pall.) Kuntze and their anti-inflammatory activity.

Bioorg Chem 2019 Apr 11;88:102922. Epub 2019 Apr 11.

Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea. Electronic address:

Dried aerial parts of Tetragonia tetragonoides were extracted with 70% EtOH, and the evaporated residue was successively separated into EtOAc, n-BuOH, and HO fractions. As a result of repeated SiO, ODS, and Sephadex LH-20 column chromatography, four new 6-methoxyflavonol glycosides (2-4, 8) along with four known ones (1, 5-7) were isolated. Several spectroscopic data led to determination of chemical structures for four new 6-methoxyflavonol glycosides (2-4, 8) and four known ones, 6-methoxykaempferol 3-O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosyl-7-O-(6‴'-(E)-caffeoyl)-β-d-glucopyranoside (1), 6-methoxyquercetin (5), 6-methoxykaempferol (6), and 6-methoxykaempferol 7-O-β-d-glucopyranoside (7). Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.102922DOI Listing

Synthesis, biological activity and multiscale molecular modeling studies of bis-coumarins as selective carbonic anhydrase IX and XII inhibitors with effective cytotoxicity against hepatocellular carcinoma.

Bioorg Chem 2019 Mar 7;87:838-850. Epub 2019 Mar 7.

Sakarya University of Applied Sciences, Pamukova Vocational Highschool, Pamukova, Turkey.

A series of novel bis-coumarin derivatives containing triazole moiety as a linker between the alkyl chains was synthesized and their inhibitory activity against the human carbonic anhydrase (hCA) isoforms I, II, IX and XII were evaluated. In addition, cytotoxic effects of the synthesized compounds on renal adenocarcinoma (769P), hepatocellular carcinoma (HepG2) and breast adeno carcinoma (MDA-MB-231) cell lines were examined. While the hCA I and II isoforms were inhibited in the micromolar range, the tumor-associated isoform hCA IX and XII were inhibited in the high nanomolar range. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.03.003DOI Listing

The challenge to predict mobilized peripheral blood stem cells on the fourth day of G-CSF treatment in healthy donors: the predictive value of basal CD34+ cell and platelet counts.

Biol Blood Marrow Transplant 2019 Apr 16. Epub 2019 Apr 16.

Institute of Clinical Physiology (IFC-CNR), Rome, Italy.

A longitudinal, prospective, observational, single-center, cohort study on healthy donors (HDs) was designed to identify predictors of CD34+ cell mobilization on day 4 after G-CSF administration. As potential predictors of mobilization, age, sex, body weight, height, blood volume, as well as white blood cell count (WBC), peripheral blood (PB) mononuclear cells (PBMC) count, platelet (Plt) count, hematocrit, and hemoglobin levels were considered. Two different evaluations of CD34+ cell counts were determined for each donor: baseline (before granulocyte colony-stimulating factor [G-CSF] administration) and in PB on day 4 after G-CSF administration. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.04.011DOI Listing

Population Pharmacokinetics of Clofarabine as Part of Pre-Transplant Conditioning in Pediatric Subjects Prior to Hematopoietic Cell Transplantation (HCT).

Biol Blood Marrow Transplant 2019 Apr 16. Epub 2019 Apr 16.

Department of Pediatrics, Division of Allergy, Immunology, and Bone Marrow Transplantation, University of California San Francisco, San Francisco, CA; Department of Clinical Pharmacy, University of California San Francisco, San Francisco, CA.

The primary objective of this work was to characterize the pharmacokinetics (PK) of systemic clofarabine (clo-fara) in pediatric allogeneic hematopoietic cell transplantation (HCT) recipients receiving either nucleoside monotherapy or a dual nucleoside analogue preparative regimen. Fifty-one children (median age 4.9 years, range 0. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.04.017DOI Listing

Safety-Engineered Syringes: An Intervention to Decrease Hepatitis C Burden in Developing Countries-A Cost-Effectiveness Analysis from Egypt.

Value Health Reg Issues 2019 Apr 16;19:51-58. Epub 2019 Apr 16.

HTA Office, L.L.C., Cairo, Egypt; Pharmacy Practice Department, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt. Electronic address:

Introduction: To assess the cost-effectiveness of introducing the safety-engineered syringe (SES) to decrease hepatitis C burden resultant from unsafe injection practices in healthcare settings.

Methods: A Markov process model for a hypothetical study cohort was developed over a 30-year time horizon to compare the adoption of SES use with the current strategy, conventional syringes (CS), in the Egyptian healthcare settings. The national treatment program was applied in both groups. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22121099193003
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http://dx.doi.org/10.1016/j.vhri.2018.11.009DOI Listing
April 2019
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[18F]fludeoxyglucose PET/CT in small-cell lung cancer: Analysis of the CONVERT randomized controlled trial.

J Thorac Oncol 2019 Apr 16. Epub 2019 Apr 16.

Division of Cancer Sciences, University of Manchester, Manchester, UK; Department of Clinical Oncology, The Christie NHS Foundation Trust, Manchester, UK.

Introduction: We used phase-3 CONVERT trial data to investigate the impact of fludeoxyglucose (F-FDG) PET/CT in small-cell lung cancer (SCLC).

Methods: CONVERT randomized limited-stage SCLC patients to twice-daily (45Gy in 30-fractions) or once-daily (66Gy in 33-fractions) chemoradiotherapy. Patients were divided into 2 groups in this unplanned analysis: those staged with conventional imaging (contrast-enhanced thorax and abdomen CT and brain imaging with/without bone scintigraphy) and those staged with F-FDG PET/CT in addition. Read More

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http://dx.doi.org/10.1016/j.jtho.2019.03.023DOI Listing

The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.

Int J Pharm 2019 Apr 16. Epub 2019 Apr 16.

Department of Pharmacy, COMSATS University Islamabad, Lahore Campus 54000, Pakistan. Electronic address:

Aim: The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).

Methodology: The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique. In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03785173193030
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http://dx.doi.org/10.1016/j.ijpharm.2019.04.046DOI Listing
April 2019
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New folate receptor targeted nano liposomes for delivery of 5-fluorouracil to cancer cells: Strong implication for enhanced potency and safety.

Life Sci 2019 Apr 16. Epub 2019 Apr 16.

Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:

We previously showed that folate liposomes of 5FU made from Dipalmitoylphosphatidylcholine (DPPC) induced cell death in HT-29 and HeLa cells more potently than bulk 5FU. Also, a primary 5FU liposomal formulation with phosphatidyl choline (PC) exhibited higher cytotoxicity in murine colon cancer cells. In the present study, optimization of 5FU PC liposome, mechanism of cell death induction in human cancer cell lines and its safety along with other assays have been employed for targeted PC liposomes of 5FU. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.04.030DOI Listing

Ca-PKCα-ERK1/2 signaling pathway is involved in the suppressive effect of propofol on proliferation of neural stem cells from the neonatal rat hippocampus.

Brain Res Bull 2019 Apr 16. Epub 2019 Apr 16.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, PR China. Electronic address:

Neonatal exposure to propofol induces persistent behavioral abnormalities in adulthood. In addition to triggering the apoptosis of neurons in the developing brain, anesthetics may contribute to the development of cognitive deficits by interfering neurogenesis. Given the importance of neural stem cell (NSC) proliferation in neurogenesis, the effect of propofol on NSC proliferation and the mechanisms underlying this effect were investigated. Read More

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http://dx.doi.org/10.1016/j.brainresbull.2019.04.005DOI Listing

Pregabalin affords retinal neuroprotection in diabetic rats: Suppression of retinal glutamate, microglia cell expression and apoptotic cell death.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Pharmacology and Toxicology, Faculty of Pharmacy (girls), Al-Azhar University, Cairo, Egypt.

Pregabalin is the first drug to receive FDA approval for treating diabetic neuropathic pain. This study investigated the neuroprotective effect of pregabalin in an experimental model of diabetic retinopathy and tested some possible mechanisms underlying the putative neuroprotective effect. Male Wistar rats received streptozotocin (45 mg/kg) to induce type 1 diabetes mellitus. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.014DOI Listing

Pharmacokinetics and tissue distribution of pilocarpine after eyelid skin application to rats.

J Pharm Sci 2019 Apr 16. Epub 2019 Apr 16.

Graduate School of Pharmaceutical Sciences; Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan. Electronic address:

Extending the delivery of drugs into the eyes while reducing systemic bioavailability is of utmost importance in the management of chronic ocular diseases. Topical application onto the lower eyelid skin, as an alternative to eye drops, is seen to be a valuable strategy in the treatment of chronic eye diseases. To elucidate the critical value of delivering drugs in solution onto the eyeball through the eyelid skin, pharmacokinetic studies of pilocarpine were conducted, and the results were verified using a direct pharmacodynamic study in rats. Read More

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http://dx.doi.org/10.1016/j.xphs.2019.04.012DOI Listing

Copper transporter 1 (CTR1) expression by mouse testicular germ cells, but not Sertoli cells, is essential for functional spermatogenesis.

PLoS One 2019 19;14(4):e0215522. Epub 2019 Apr 19.

Institute of Cellular and Molecular Biology, College of Natural Sciences, The University of Texas at Austin, Austin, TX, United States of America.

An imbalance in copper (Cu) tissue homeostasis has a degenerative effect on spermatogenesis and male fertility. The high-affinity Cu transporter 1 (CTR1; SLC31A1) is the major protein responsible for Cu acquisition in eukaryotes and is highly expressed in mouse testes. Studies on yeast and Drosophila have demonstrated the conserved essential function of Cu and CTR1 for meiosis and fertility, implying that CTR1 may play an essential function in mammalian spermatogenesis. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215522PLOS

Productivity growth of skilled nursing facilities in the treatment of post-acute-care-intensive conditions.

PLoS One 2019 19;14(4):e0215876. Epub 2019 Apr 19.

School of Pharmacy, University of Southern California, Los Angeles, California, United States of America.

Background: Health care is believed to be suffered from a "cost disease," in which a heavy reliance on labor limits opportunities for efficiencies stemming from technological improvement. Although recent evidence shows that U.S. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215876PLOS

Aniracetam does not improve working memory in neurologically healthy pigeons.

PLoS One 2019 19;14(4):e0215612. Epub 2019 Apr 19.

Department of Psychology, University of Otago, Dunedin, Otago, New Zealand.

Understanding the effects of cognitive enhancing drugs is an important area of research. Much of the research, however, has focused on restoring memory following some sort of disruption to the brain, such as damage or injections of scopolamine. Aniracetam is a positive AMPA-receptor modulator that has shown promise for improving memory under conditions when the brain has been damaged, but its effectiveness in improving memory in neurologically healthy subjects is unclear. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215612PLOS

Doxycycline, Azithromycin and Vitamin C (DAV): A potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs).

Aging (Albany NY) 2019 Apr 19. Epub 2019 Apr 19.

Translational Medicine, School of Environment and Life Sciences, Biomedical Research Centre (BRC), University of Salford, Greater Manchester, M5 4WT, United Kingdom.

Here, we devised a new strategy for eradicating cancer stem cells (CSCs), via a "synthetic-metabolic" approach, involving two FDA-approved antibiotics and a dietary vitamin supplement. This approach was designed to induce a "rho-zero-like" phenotype in cancer cells. This strategy effectively results in the synergistic eradication of CSCs, using vanishingly small quantities of two antibiotics. Read More

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http://dx.doi.org/10.18632/aging.101905DOI Listing

Assessing the Bioavailability of Zinc and Indole Compounds from Mycelial Cultures of the Bay Mushroom Imleria badia (Agaricomycetes) Using In Vitro Models.

Int J Med Mushrooms 2019 ;21(4):343-352

Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.

Zinc and indole compounds demonstrate anti-inflammatory, antidepressant, and antioxidant activity. Edible mushrooms are good sources of these substances. Therefore, in this study, we aimed to study the accumulation, release, and absorption of zinc and indole compounds from mycelial cultures of Imleria badia species using in vitro models. Read More

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http://dx.doi.org/10.1615/IntJMedMushrooms.2019030328DOI Listing
January 2019

Role of Aqueous Extract of the Wood Ear Mushroom, Auricularia polytricha (Agaricomycetes), in Avoidance of Haloperidol-lnduced Catalepsy via Oxidative Stress in Rats.

Int J Med Mushrooms 2019 ;21(4):323-330

School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, 302017, Jaipur, India.

Haloperidol-induced catalepsy is an animal model of a psychotic disorder that may be associated with neurodegeneration and free radical damage. Auricularia polytricha is effective in both prevention and treatment of numerous types of neurological disorders. In the present study, anticataleptic activity of aqueous extract of A polytricha (AEAP) at different doses (400 and 600 mg/kg, respectively, p. Read More

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http://dx.doi.org/10.1615/IntJMedMushrooms.2019030351DOI Listing
January 2019
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Analysis of Intraspecies Genetic Variability among Collections of Medicinal Red Belt Conk Mushroom, Fomitopsis pinicola (Agaricomycetes).

Int J Med Mushrooms 2019 ;21(3):291-300

Department of Mycology and Algology, Faculty of Biology, Moscow Lomonosov State University, Moscow, Russia.

Intraspecies genetic variability of the medicinal dikaryotic polypore mushroom Fomitopsis pinicola was analyzed by using variable internal transcribed space (ITS) region of the ribosomal DNA gene cluster and the somatic compatibility test. The results revealed very low ITS sequence polymorphism among strains of F. pinicola from geographically distant origins (Russia, Finland, and France). Read More

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http://dx.doi.org/10.1615/IntJMedMushrooms.2019030083DOI Listing
January 2019

Chemical Compounds and Antioxidant Activity of Volatile Oil from the White Jelly Mushroom, Tremella fuciformis (Tremellomycetes).

Int J Med Mushrooms 2019 ;21(3):207-214

National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai, China; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.

To fully analyze the composition of volatile oil extracted from Tremella fuciformis, hydrodistillation (HD) and solid phase microextraction (SPME) were adopted simultaneously. In both cases, the analysis was carried out using gas chromatography-mass spectrometry and the antioxidant activity of the volatile oil was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with rutin as a positive control. Nineteen components in HD and 68 components in SPME were identified, respectively. Read More

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http://dx.doi.org/10.1615/IntJMedMushrooms.2019030099DOI Listing
January 2019

Computational Modeling of Bone Cells and Their Biomechanical Behaviors in Responses to Mechanical Stimuli.

Crit Rev Eukaryot Gene Expr 2019 ;29(1):51-67

Department of Hand surgery, Department of Plastic Reconstructive Surgery, Ningbo No. 6 Hospital, Ningbo, 315040, China; School of Pharmacy and Medical Sciences, and UniSA Cancer Research Institute, University of South Australia, Adelaide, SA 5001, Australia.

Bone cells, including osteoblasts, osteoclasts, and osteocytes, have the ability to develop and maintain bone architecture. Although improved experimental testing approaches are increasing our understanding of the complex structures and functions of bone cells and bone, computational models, particularly finite element analyses, are being used to extend this knowledge and to develop a more theoretical understanding of bone cell behaviors. There are many challenges to developing an accurate and validated computational model due to the complex structure and biomechanical behaviors of the bone cells and bone tissue. Read More

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http://dx.doi.org/10.1615/CritRevEukaryotGeneExpr.2019025150DOI Listing
January 2019

Gene Delivery Using Lipoplexes and Polyplexes: Principles, Limitations and Solutions.

Crit Rev Eukaryot Gene Expr 2019 ;29(1):29-36

Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Gene therapy has attracted considerable attention for the treatment of genetic and acquired diseases. Successful gene therapy occurs when the therapeutic genes penetrate targeted cells and become available to the intracellular active site. Currently, a promising approach in gene delivery is the use of nonviral gene delivery vectors that lack immunogenicity but have low toxicity and potential tissue specificity. Read More

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http://dx.doi.org/10.1615/CritRevEukaryotGeneExpr.2018025132DOI Listing
January 2019

The Role of Melanin in Skin Cancer.

Crit Rev Eukaryot Gene Expr 2019 ;29(1):17-24

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Low melanin level in white skin results in many genetic alterations that activate oncogenes to form metastatic melanomas because of interaction with ultraviolet rays. These melanomas are uncommon, but they are dangerous and spread rapidly in the individual's body. Individuals with fair, freckled skin; a weak immune system; or have a personal or family history of melanoma are at high risk to have melanoma. Read More

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http://dx.doi.org/10.1615/CritRevEukaryotGeneExpr.2018024980DOI Listing
January 2019

Pediatric drug safety signal detection of non-chemotherapy drug-induced neutropenia and agranulocytosis using electronic healthcare records.

Expert Opin Drug Saf 2019 Apr 19:1-7. Epub 2019 Apr 19.

a Clinical Research Center , National Center for Children's Health, Beijing Children's Hospital, Capital Medical University , Beijing , China.

Objectives: This study aimed to develop a procedure to explore the adverse drug reaction signals of drug-induced neutropenia (DIN) or drug-induced agranulocytosis (DIA) in children using an electronic health records (EHRs) database.

Methods: A two-stage design was presented. First, the suspected drugs to induce DIN or DIA were selected. Read More

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http://dx.doi.org/10.1080/14740338.2019.1604682DOI Listing

Managing costs and care for chronic idiopathic constipation.

Authors:
Dana McCormick

Am J Manag Care 2019 Mar;25(4 Suppl):S63-S69

Director of Pharmacy, Blue Cross Blue Shield of Texas, Richardson, TX. Email:

For decades, chronic idiopathic constipation (CIC) has been one of the most common chronic functional gastrointestinal disorders encountered by clinicians. Common comorbidities include depression, diabetes, functional dyspepsia, hypothyroidism, overweight, obesity, and neurological disorders. CIC imposes a large economic burden on the American healthcare system with estimated costs of $1912 to $7522 in 2007 US dollars per patient per year. Read More

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March 2019
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Novel Measures of Benzodiazepine and Z-Drug Utilisation Trends in a Canadian Provincial Adult Population (2001-2016).

J Popul Ther Clin Pharmacol 2019 Jan 22;26(1):e22-e38. Epub 2019 Jan 22.

College of Pharmacy, University of Manitoba.

Purpose:  1) To evaluate trends for benzodiazepines (BZD) and Z-Drugs over 15-years in a general Canadian adult population measured by: a) consumption b) pharmacologic exposure c) dose intensity and d) prevalence of use. 2) To demonstrate the utility of Diazepam Milligram Equivalence (DME) based measurements when used in conjunction with traditional standard measurements of drug utilization such as the Defined Daily Dose (DDD) system.

Methods: Administrative data covering all prescriptions from April 2001-March 2016 for BZD and Z-Drugs for patients ≥18 years was used. Read More

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http://www.jptcp.com/index.php/jptcp/article/view/52
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http://dx.doi.org/10.22374/1710-6222.26.1.3DOI Listing
January 2019
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Multiplex Touchdown PCR assay to enhance specificity and sensitivity for concurrent detection of four foodborne pathogens in raw milk.

J Appl Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Department of Medicinal Chemistry, Faculty of Pharmacy, Shiraz, Iran.

Aims: The aim of this study was to develop a multiplex touchdown PCR (multiplex TD-PCR) for rapid and simultaneous detection of four major foodborne pathogens to avoid mispriming and unwanted production during gene amplification. Touchdown PCR is the modified form of standard PCR, which enhances specificity, sensitivity.

Methods And Results: For this reason, a multiplex TD-PCR assay with a pre-enrichment step was developed to detect four foodborne pathogens namely Escherichia coli O157:H7, Listeria monocytogenes, Staphylococcus aureus, and Salmonella enterica serovar Enteritidis in pure culture and raw milk samples. Read More

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http://dx.doi.org/10.1111/jam.14285DOI Listing

Molecular design opportunities presented by solvent-exposed regions of target proteins.

Med Res Rev 2019 Apr 19. Epub 2019 Apr 19.

Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, People's Republic of China.

Solvent-exposed regions, or solvent-filled pockets, within or adjacent to the ligand-binding sites of drug-target proteins provide opportunities for substantial modifications of existing small-molecular drug molecules without serious loss of activity. In this review, we present recent selected examples of exploitation of solvent-exposed regions of proteins in drug design and development from the recent medicinal-chemistry literature. Read More

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http://dx.doi.org/10.1002/med.21581DOI Listing

Population modeling highlights drug disposition differences between tenofovir alafenamide and tenofovir disoproxil fumarate in the blood and semen.

Clin Pharmacol Ther 2019 Apr 19. Epub 2019 Apr 19.

Unc Eshelman School Of Pharmacy, Division Of Pharmacotherapy And Experimental Therapeutics, University Of North Carolina At Chapel Hill, Chapel Hill, NC.

Understanding antiretroviral disposition in the male genital tract, a distinct viral compartment, can provide insight for HIV eradication. Population pharmacokinetic modeling was conducted to investigate the disposition of tenofovir disoproxil fumarate (TDF), tenofovir alafenamide (TAF), and emtricitabine and their metabolites in blood and semen. Blood plasma and seminal plasma (SP) concentrations of tenofovir and emtricitabine, and tenofovir-diphosphate and emtricitabine-triphosphate concentrations in the peripheral blood mononuclear cells (PBMC) and seminal mononuclear cells, were measured. Read More

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http://dx.doi.org/10.1002/cpt.1464DOI Listing

Use of 4β-hydroxycholesterol plasma concentrations as an endogenous biomarker of CYP3A activity: Clinical validation in individuals with type 2 diabetes.

Clin Pharmacol Ther 2019 Apr 19. Epub 2019 Apr 19.

Faculty of Pharmacy, Université de Montréal, Montreal, QC, Canada.

The relevance of endogenous 4β-hydroxycholesterol (4β-OHC) plasma concentrations or of the 4β-OHC/total cholesterol concentration ratio (4β-OHC ratio) as surrogate markers of CYP3A activity was evaluated in individuals with (n=38) or without (n=35) type 2 diabetes (T2D). Midazolam was used as a comparator to validate exploratory measures of phenotypic CYP3A activity. Metabolic ratios (MR) of orally administered midazolam in the non-diabetic and diabetic populations correlated significantly with 4β-OHC (r =0. Read More

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http://dx.doi.org/10.1002/cpt.1472DOI Listing

Effect of electrochemotherapy with betulinic acid or cisplatin on regulation of heat shock proteins in metastatic human carcinoma cells in vitro.

Oncol Rep 2019 Apr 8. Epub 2019 Apr 8.

Department of Molecular and Cellular Biology, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, 50‑556 Wroclaw, Poland.

Betulinic acid (BTA) is naturally occurring triterpene that has received interest as a novel therapeutic substance with cytotoxicity towards a number of cancer cell lines. Despite the wide spectrum of biological and pharmacological effects, its effect may be limited its lipophobic properties. Therefore, strategies to improve the access of BTA to the cells are required to enhance the anticancer effects. Read More

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http://dx.doi.org/10.3892/or.2019.7103DOI Listing

JS‑K induces G2/M phase cell cycle arrest and apoptosis in A549 and H460 cells via the p53/p21WAF1/CIP1 and p27KIP1 pathways.

Oncol Rep 2019 Apr 9. Epub 2019 Apr 9.

Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.

Lung cancer is one of the most common malignancies worldwide, with high mortality and morbidity rates. O2‑​(2,4‑​dinitrophenyl)‑1‑​[(4‑ethoxycarbonyl)piperazin‑1‑yl]diazen‑1‑ium‑1,2‑diolate (JS‑K) is a potent anticancer agent that acts against a subset of human non‑small cell lung cancer (NSCLC) cell lines; however, the underlying mechanisms of JS‑K in NSCLC remain unclear. The present study aimed to evaluate the anticancer effect of JS‑K and investigate its underlying mechanisms in A549 and H460 cells. Read More

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http://dx.doi.org/10.3892/or.2019.7104DOI Listing

Association Between Pharmacy Closures and Adherence to Cardiovascular Medications Among Older US Adults.

JAMA Netw Open 2019 Apr 5;2(4):e192606. Epub 2019 Apr 5.

Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Importance: It is unknown whether and how pharmacy closures alter medication adherence.

Objective: To examine the association between pharmacy closures and adherence to statins, β-blockers, and oral anticoagulants among adults 50 years or older in the United States.

Design, Setting, And Participants: In this retrospective cohort study, comparative interrupted time series analyses were performed using a nationally representative 5% random sample of anonymized, longitudinal, individual-level pharmacy claims from IQVIA LRx LifeLink. Read More

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http://dx.doi.org/10.1001/jamanetworkopen.2019.2606DOI Listing

Reversal of carbapenemase-producing Klebsiella pneumoniae epidemiology from blaKPC- to blaVIM-harbouring isolates in a Greek ICU after introduction of ceftazidime/avibactam.

J Antimicrob Chemother 2019 Apr 19. Epub 2019 Apr 19.

Department of Microbiology, School of Medicine, University of Patras, 26504, Rion-Patras, Greece.

Objectives: Our aim was to determine the epidemiology of bloodstream infections (BSIs) by carbapenemase-producing Klebsiella pneumoniae (CP-Kp) after the introduction of ceftazidime/avibactam in January 2018 among ICU patients.

Patients And Methods: All patients hospitalized at the ICU of the University General Hospital of Patras, Greece with CP-Kp BSI during 2015-18 were included. MICs of meropenem, fosfomycin, tigecycline and ceftazidime/avibactam (only for isolates from 2018) were determined by Etest, whereas for colistin, the broth microdilution method was applied. Read More

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http://dx.doi.org/10.1093/jac/dkz125DOI Listing

Association Between Secondary Prevention Medication Use and Outcomes in Frail Older Adults After Acute Myocardial Infarction.

Circ Cardiovasc Qual Outcomes 2019 Apr;12(4):e004942

Division of Geriatrics, University of California, San Francisco and San Francisco VA Medical Center, San Francisco (M.A.S., S.J.L.).

Background Secondary prevention medications are often not prescribed to frail, older adults following acute myocardial infarction, potentially because of the absence of data to support use, perceived lack of benefit, and concern over possible harms. We examined the effect of using more guideline-recommended medications after myocardial infarction on mortality, rehospitalization, and functional decline in the frailest and oldest segment of the US population-long-stay nursing home residents. Methods and Results We conducted a retrospective cohort study of nursing home residents aged ≥65 years using 2007 to 2010 national US Minimum Data Set clinical assessment data and Medicare claims. Read More

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https://www.ahajournals.org/doi/10.1161/CIRCOUTCOMES.118.004
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http://dx.doi.org/10.1161/CIRCOUTCOMES.118.004942DOI Listing
April 2019
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Lycopene protects myocardial ischemia injury through anti-apoptosis and anti-oxidative stress.

Eur Rev Med Pharmacol Sci 2019 Apr;23(7):3096-3104

Department of Pharmacy, Emergency General Hospital, Beijing, China.

Objective: The aim of this research was to explore the protective effect of lycopene (Lyc) on myocardial ischemia injury through anti-apoptosis and anti-oxidative stress.

Materials And Methods: 75 rats were divided into 5 groups: sham operation group (control group), model group, low-dose group (Lyc+2 mg/kg), medium-dose group (Lyc+4 mg/kg) and high-dose group (Lyc+6 mg/kg). The rat model of myocardial ischemia was established by a subcutaneous injection of isoproterenol (85 mg/kg) for two consecutive days. Read More

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http://www.europeanreview.org/article/17593
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http://dx.doi.org/10.26355/eurrev_201904_17593DOI Listing
April 2019
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Oral delivery of nanoparticles - let's not forget about the protein corona.

Expert Opin Drug Deliv 2019 Apr 19. Epub 2019 Apr 19.

b Laboratory of Supramolecular and BioNano Materials (SupraBioNanoLab), Department of Chemistry , Materials and Chemical Engineering , Politecnico di Milano, Milano , Italy.

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http://dx.doi.org/10.1080/17425247.2019.1610384DOI Listing
April 2019
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Vagiallene, a Rearranged C Acetogenin from Laurencia obtusa.

Org Lett 2019 Apr 19. Epub 2019 Apr 19.

Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy , National and Kapodistrian University of Athens , Panepistimiopolis Zografou , Athens 15771 , Greece.

Vagiallene (1), a rearranged C acetogenin with a molecular formula and a carbon skeleton unprecedented in natural products, was isolated as a trace constituent from the organic extract of the red alga Laurencia obtusa from Lefkada island. The planar structure and the relative configuration of 1 were established on the basis of extensive analysis of its spectroscopic data, while its absolute configuration was determined by comparison of its experimental and quantum-mechanically predicted electronic circular dichroism spectra. Read More

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http://dx.doi.org/10.1021/acs.orglett.9b00897DOI Listing

Sulforaphane Potentially Ameliorates Arsenic Induced Hepatotoxicity in Albino Wistar Rats: Implication of PI3K/Akt/Nrf2 Signaling Pathway.

Cell Physiol Biochem 2019 ;52(5):1203-1222

Department of Zoology, Bharathiar University, Coimbatore, India.

Background/aims: Agriculture and industrial expansion in recent years have resulted in the undue discharge of arsenic into the environment, building arsenic toxicity a major worldwide anxiety. Oxidative stress is considered as the most conspicuous effect of arsenic toxicity. The current study was designed to evaluate the protective ability of sulforaphane (SFN) against arsenic (As) induced hepatotoxicity by activation of PI3K induced Akt and Nrf2 mediated signaling pathway. Read More

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http://dx.doi.org/10.33594/000000082DOI Listing
January 2019

[Development of an ELISA-based high throughput screening method for novel anticancer agents targeting β-catenin/Lef1 interaction].

Sheng Wu Gong Cheng Xue Bao 2019 Apr;35(4):707-717

Center for New Drug Screening & Evaluation, School of Pharmacy, Wannan Medical College, Wuhu 241002, Anhui, China.

To develop an enzyme-linked immunosorbent assay (ELISA)-based high throughput screening (HTS) method for β-catenin/Lef1 interaction antagonists screening, Escherichia coli Rosetta (DE3) competent cells were transformed with β-catenin-pET-30a(+) plasmid. β-catenin protein was expressed after induction and purified using affinity chromatography. The biological activity of purified β-catenin was further analyzed by GST Pulldown assay. Read More

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http://www.cnki.net/kcms/doi/10.13345/j.cjb.180344.html
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http://dx.doi.org/10.13345/j.cjb.180344DOI Listing
April 2019
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Neuroprotective Effects of Zafirlukast, Piracetam and Their Combination on L-Methionine-Induced Vascular Dementia in Rats.

Fundam Clin Pharmacol 2019 Apr 18. Epub 2019 Apr 18.

Pharmacology Department, October University for Modern Science and Arts, 11787, 6, October City, Egypt.

Vascular dementia is considered a vascular cognitive impairment disease caused by neuronal degeneration in the brain. Several studies have supported the hypothesis that oxidative stress and endothelial dysfunction are the main pathogenic factors in vascular dementia. This current study aims to determine the possible neuroprotective effects of zafirlukast, piracetam and the combination of piracetam and zafirlukast on L-methionine-induced vascular dementia in rats. Read More

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http://dx.doi.org/10.1111/fcp.12473DOI Listing

Mesoporous silica nanoparticles, a safe option for silymarin delivery: preparation, characterization, and in vivo evaluation.

Drug Deliv Transl Res 2019 Apr 18. Epub 2019 Apr 18.

Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.

The present work aimed to prepare silymarin-loaded mesoporous silica nanoparticles (MSNs) and to assess the system's dissolution enhancement ability on the pharmacodynamic performance of silymarin as a hepatoprotective agent. For this purpose, a soft-templating technique was used to prepare silymarin-loaded MSNs. The loaded MSNs were further characterized for their particle size, zeta potential, surface properties, and in vitro drug dissolution testing. Read More

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http://dx.doi.org/10.1007/s13346-019-00640-3DOI Listing

Translation Between Two Models; Application with Integrated Glucose Homeostasis Models.

Pharm Res 2019 Apr 17;36(6):86. Epub 2019 Apr 17.

Department of Pharmaceutical Biosciences, Uppsala University, Box 591, 751 23, Uppsala, Sweden.

Purpose: For some biological systems, there exist several models with somewhat different features and perspectives. We propose an evaluation method for NLME models by analyzing real and simulated data from the model of main interest using a structurally different, but similar, NLME model. We showcase this method using the Integrated Glucose Insulin (IGI) model and the Integrated Minimal Model (IMM). Read More

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http://dx.doi.org/10.1007/s11095-019-2592-9DOI Listing

Canagliflozin reduces inflammation and fibrosis biomarkers: a potential mechanism of action for beneficial effects of SGLT2 inhibitors in diabetic kidney disease.

Diabetologia 2019 Apr 17. Epub 2019 Apr 17.

Department of Internal Medicine IV (Nephrology and Hypertension), Medical University of Innsbruck, Innsbruck, Austria.

Aims/hypothesis: The sodium-glucose cotransporter 2 (SGLT2) inhibitor canagliflozin slows progression of kidney function decline in type 2 diabetes. The aim of this study was to assess the effect of the SGLT2 inhibitor canagliflozin on biomarkers for progression of diabetic kidney disease (DKD).

Methods: A canagliflozin mechanism of action (MoA) network model was constructed based on an in vitro transcriptomics experiment in human proximal tubular cells and molecular features linked to SGLT2 inhibitors from scientific literature. Read More

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http://dx.doi.org/10.1007/s00125-019-4859-4DOI Listing

Safety aspects of antiepileptic drugs-a population-based study of adverse effects relative to changes in utilisation.

Eur J Clin Pharmacol 2019 Apr 18. Epub 2019 Apr 18.

Programme for Pharmacy, Faculty of Health Sciences, Oslo Metropolitan University, Oslo, Norway.

Purpose: Antiepileptic drugs (AEDs) are increasingly used, and knowledge about adverse effects is scarce based on clinical studies. The purpose of the present study was to characterise adverse effects reports of AEDs in Norway relative to changes in utilisation in various indications from population-based data to elucidate important safety aspects of use of AEDs.

Methods: Aggregated data of adverse effects reported for AEDs in Norway from the EudraVigilance-database (2004-2013) in addition to indication-specific use of AEDs during 2004-2015 from the Norwegian Prescription Database were used. Read More

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http://dx.doi.org/10.1007/s00228-019-02678-1DOI Listing
April 2019
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Comparative analyses of plasma amyloid-β levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system.

Sci Adv 2019 Apr 17;5(4):eaav1388. Epub 2019 Apr 17.

Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.

Detection of amyloid-β (Aβ) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Aβ is deemed a less invasive and more accessible hallmark of AD, as Aβ can penetrate blood-brain barriers. However, correlations between biofluidic Aβ concentrations and AD progression has been tenuous. Read More

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http://dx.doi.org/10.1126/sciadv.aav1388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469948PMC