12,540 results match your criteria Journal of pharmaceutical and biomedical analysis[Journal]


Therapeutic drug monitoring of teicoplanin using an LC-MS/MS method: Analysis of 421 measurements in a naturalistic clinical setting.

J Pharm Biomed Anal 2019 Feb 4;167:161-165. Epub 2019 Feb 4.

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea; Department of Clinical Pharmacology & Therapeutics, Samsung Medical Center, Seoul, Republic of Korea. Electronic address:

Teicoplanin is a glycopeptide antibiotic used for treatment of severe Gram-positive bacterial infection. The aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for therapeutic drug monitoring (TDM) of teicoplanin and to review our clinical experience. We established an LC-MS/MS method to analyze serum concentration of teicoplanin using simple protein precipitation with a 5 min run time for each sample. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.001DOI Listing
February 2019

Validation of LC-MS/MS methods for the determination of mirabegron and eight metabolites in human plasma in a paediatric population.

J Pharm Biomed Anal 2019 Feb 7;167:155-160. Epub 2019 Feb 7.

Astellas Pharma Europe B.V., Leiden, The Netherlands.

Mirabegron is the first registered β3-adrenoceptor agonist for treatment of overactive bladder as an alternative to antimuscarinics. Previously four liquid chromatography-tandem mass spectrometry assays were published to determine mirabegron and eight metabolites (M5, M8, M11-M16) in human plasma. In order to support paediatric development, the assays were further optimized to reduce the required blood volume and increase the sensitivity. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.007DOI Listing
February 2019

Role of esterase mediated hydrolysis of simvastatin in human and rat blood and its impact on pharmacokinetic profiles of simvastatin and its active metabolite in rat.

J Pharm Biomed Anal 2019 Feb 6;168:13-22. Epub 2019 Feb 6.

School of Pharmacy, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong Special Administrative Region. Electronic address:

Simvastatin is known as a pro-drug, which could be hydrolyzed by esterases to its active form, simvastatin acid. Although pharmacokinetics of simvastatin and simvastatin acid have been widely studied, hydrolysis of simvastatin to simvastatin acid during blood sampling and plasma preparation has been overlooked in the previous studies, leading to underestimation of simvastatin concentration and overestimation of simvastatin acid concentration in plasma. Since both efficacy and adverse drug reaction of simvastatin are highly dependent on simvastatin and simvastatin acid concentrations in vivo, accurate assessment of the two compounds are critical in their pharmacokinetic and pharmacodynamic studies. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.004DOI Listing
February 2019

Phytochemical profile of Syzygium formosum (Wall.) Masam leaves using HPLC-PDA-MS/MS and a simple HPLC-ELSD method for quality control.

J Pharm Biomed Anal 2019 Feb 11;168:1-12. Epub 2019 Feb 11.

College of Pharmacy, Chungnam National University, Daejeon 3414, South Korea. Electronic address:

Syzygium formosum (SF) leaves have been used for thousands of years in traditional Vietnamese medicine for the treatment of allergy or skin rash. However, the role of the phytochemical profile of SF leaves on their activities is poorly understood. Additionally, there is currently no quality control method for this herbal material, which is required by the pharmaceutical industry. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.014DOI Listing
February 2019

Development of the LC-MS/MS method for determining the p-cresol level in plasma.

J Pharm Biomed Anal 2019 Jan 31;167:149-154. Epub 2019 Jan 31.

Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland. Electronic address:

p-Cresol is a protein-bound uremic retention solute that originates in the intestine through bacterial metabolism and accumulates throughout the body in case of kidney failure. To date, there has been no method to analyze unconjugated p-cresol concentration in the blood with a limit of detection lower than 75 pg. Thus, the aim of this study was to develop and validate a novel liquid chromatography-tandem mass spectrometry method for the determination of unconjugated p-cresol in plasma with a lower detection limit than what has been determined using previously described methods. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.041DOI Listing
January 2019

Metabolic shift of Staphylococcus aureus under sublethal dose of methicillin in the presence of glucose.

J Pharm Biomed Anal 2019 Feb 8;167:140-148. Epub 2019 Feb 8.

Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 45056, USA; Department of Human Sciences, The Ohio State University, Columbus, OH, 43210, USA. Electronic address:

Traditional strategies in developing novel drugs to treat antibiotic-resistant S. aureus have not been very successful to date, therefore, there is an urgent need for creative usage of existing agents that can treat and control S. aureus infection. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183247
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http://dx.doi.org/10.1016/j.jpba.2019.02.010DOI Listing
February 2019
2 Reads

Application of integrative cloud point extraction and concentration for the analysis of polyphenols and alkaloids in mulberry leaves.

J Pharm Biomed Anal 2019 Feb 10;167:132-139. Epub 2019 Feb 10.

Northeast Forestry University, Hexing Road 26, 150040, Harbin, PR China. Electronic address:

A simple and efficient method based on cloud point extraction and concentration combined with high performance liquid chromatography was developed for the simultaneous separation and determination of five target compounds (deoxynojirimycin, chlorogenic acid, rutin, isoquercitrin and astragalin) in mulberry leaves samples. Firstly, to obtain a high extraction rate, the ultrasound assisted extraction was developed on acid modified Triton X-114 system. Under the optimal conditions, the total maximum extraction yields of five target compounds reached 20. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.002DOI Listing
February 2019
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Identification and characterization of unknown degradation impurities in beclomethasone dipropionate cream formulation using HPLC, ESI-MS and NMR.

J Pharm Biomed Anal 2019 Feb 10;167:123-131. Epub 2019 Feb 10.

Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung City, Taiwan; Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan. Electronic address:

The present study focuses on identifying the degradation profile and pathways of unknown impurities from beclomethasone dipropionate (BDP) topical cream formulation reported under accelerated stability conditions. Six degradation impurities were observed during the accelerated stability testing of BDP topical cream formulation, and these thermally labile degradation impurities were primarily identified using a simple, effective and mass compatible isocratic reversed-phase high-performance liquid chromatography with ultraviolet detection method. The degradation impurities found in this sample were of very low concentration levels, thus the concentration of these impurities in the sample was enriched by mimicking the thermal degradation conditions to structurally elucidate the unknown impurities. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.013DOI Listing
February 2019

In vitro metabolic profiles of motolimod by using liquid chromatography tandem mass spectrometry: Metabolic stability, metabolite characterization and species comparison.

J Pharm Biomed Anal 2019 Feb 7;167:90-99. Epub 2019 Feb 7.

Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Motolimod (VTX-2337) is an agonist of toll-like receptor 8 (TLR8) with potential immune-stimulating and antineoplastic activities. The purpose of this study was to investigate the in vitro metabolic profiles of VTX-2337. The average in vitro T values were 6. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.009DOI Listing
February 2019
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Hierarchical extraction and simultaneous determination of flavones and triterpenes in different parts of Trichosanthes kirilowii Maxim. by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry.

J Pharm Biomed Anal 2019 Feb 6;167:114-122. Epub 2019 Feb 6.

Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230001, China.

Trichosanthin (root of Trichosanthes kirilowii Maxim.) has received much attention, but T. kirilowii has received little systematic analysis. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.003DOI Listing
February 2019
1 Read

Serum metabolomics model and its metabolic characteristics in patients with different syndromes of dyslipidemia based on nuclear magnetic resonance.

J Pharm Biomed Anal 2019 Feb 4;167:100-113. Epub 2019 Feb 4.

Neurology Department (3 ward), Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. Electronic address:

Dyslipidemia is known as a common clinical disease that affects the health of millions of people around the world. The treatment of dyslipidemia with traditional Chinese medicine (TCM) is generally based on the accurate identification of disease syndromes. TCM syndromes are judged by traditional four-diagnosis method, which is subjective and fuzzy. Read More

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http://dx.doi.org/10.1016/j.jpba.2018.12.042DOI Listing
February 2019
1 Read

Urinary metabolomics for discovering metabolic biomarkers of laryngeal cancer using UPLC-QTOF/MS.

J Pharm Biomed Anal 2019 Jan 30;167:83-89. Epub 2019 Jan 30.

China National Tobacco Quality Supervision & Test Center, Zhengzhou, 45000l, China. Electronic address:

The incidence of laryngeal cancer (LYC) is second only to lung cancer, which is also the second most common cancer in head and neck cancer. Risk assessment metabolomics biomarkers to diagnose LYC have not been found by now. To profile the metabolites in healthy controls (HCs) and LYC patients (LYCs), urine metabonomics study was performed based on reversed phase liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RPLC-QTOF/MS). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183233
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http://dx.doi.org/10.1016/j.jpba.2019.01.035DOI Listing
January 2019
2 Reads

The power of hyphenated chromatography-Time of flight mass spectrometry for unequivocal identification of spirostanes in bodybuilding dietary supplements.

J Pharm Biomed Anal 2019 Feb 1;167:74-82. Epub 2019 Feb 1.

National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, MS 38677, USA. Electronic address:

A previously unidentified purported botanical ingredient was found in dietary supplements marketed for anabolic benefits. In an attempt to assess the 'naturalness' of a group of steroid-like compounds called laxogenins, a UHPLC-QToF method was developed. Several dietary supplements claim to contain 5α-hydroxy laxogenin, which is a derivative of a naturally occurring spirostane-type steroid, laxogenin. Read More

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http://dx.doi.org/10.1016/j.jpba.2018.12.045DOI Listing
February 2019
2.979 Impact Factor

Ex-vivo intestinal absorption study of boswellic acid, cyclodextrin complexes and poloxamer solid dispersions using everted gut sac technique.

J Pharm Biomed Anal 2019 Feb 1;167:66-73. Epub 2019 Feb 1.

Department of Chemistry, Sunandan Divatia School of science, SVKM's NMIMS (Deemed-to-be) University, Vile Parle-West, Mumbai, 400056, Maharashtra, India. Electronic address:

Acetyl- Keto-β-boswellic acid (AKBA) is a pentacyclic triterpenic acid found in gum resin of Boswellia serrata. Even though it is shown to have anti-inflammatory activity, its bioavailability gets limited due to its poor aqueous solubility and permeability. The present study, hence, deals in enhancement of the intestinal absorption of AKBA from total boswellic acid fraction (TA fraction) using cyclodextrin (CD) and poloxamer solid dispersion (PXM SDs) formulations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183161
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http://dx.doi.org/10.1016/j.jpba.2018.12.018DOI Listing
February 2019
3 Reads

Sniffing out resistance - Rapid identification of urinary tract infection-causing bacteria and their antibiotic susceptibility using volatile metabolite profiles.

J Pharm Biomed Anal 2019 Jan 31;167:59-65. Epub 2019 Jan 31.

Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, United Kingdom. Electronic address:

Antibiotic resistance is set to be an unprecedented threat to modern medicine. 'Sniffing' bacteria potentially offers a rapid way to determine susceptibility. A successful proof-of-principle study is described, using thermal desorption-gas chromatography-mass spectrometry (TDGCMS) to 'smell' cephalexin and ciprofloxacin resistant and sensitive Urinary Tract Infection (UTI)-causing bacteria. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183141
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http://dx.doi.org/10.1016/j.jpba.2019.01.044DOI Listing
January 2019
1 Read

Structure elucidation of novel degradation products of thiocolchicoside by NMR spectroscopy.

J Pharm Biomed Anal 2019 Jan 31;167:49-58. Epub 2019 Jan 31.

Department of Medicinal Chemistry, GVK Biosciences Pvt. Ltd, IDA Mallapur, 500076 Hyderabad, Telangana, India. Electronic address:

Thiocolchicoside is a natural product analogue often used for its spasmolytic action. To know more about its stability under various stress conditions, the drug was stirred in acid, base and peroxide solutions. In acid hydrolysis, two products were obtained and in both, the glucose got cleaved. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183290
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http://dx.doi.org/10.1016/j.jpba.2018.12.048DOI Listing
January 2019
1 Read

Development and validation of an assay for quantifying budesonide in dried blood spots collected from extremely low gestational age neonates.

J Pharm Biomed Anal 2019 Jan 29;167:7-14. Epub 2019 Jan 29.

Center for Human Toxicology, College of Pharmacy, University of Utah, Salt Lake City, UT, United States; Department of Pathology, School of Medicine, University of Utah, Salt Lake City, Utah, United States.

Budesonide is a potential therapeutic option for the prevention of bronchopulmonary dysplasia in mechanically ventilated premature neonates. The dose and concentrations of budesonide that drive effective prophylaxis are unknown, due in part to the difficulty in obtaining serial blood samples from this fragile population. Of primary concern is the limited total blood volume available for collection for the purposes of a pharmacokinetic study. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183215
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http://dx.doi.org/10.1016/j.jpba.2019.01.048DOI Listing
January 2019
2 Reads

Identification of anti-inflammatory components in Sinomenii Caulis based on spectrum-effect relationship and chemometric methods.

J Pharm Biomed Anal 2019 Jan 30;167:38-48. Epub 2019 Jan 30.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, PR China. Electronic address:

The present study aimed to identify the anti-inflammatory components in Sinomenii Caulis (SC) based on spectrum-effect relationship and chemometric methods. A phytochemical investigation of SC extract was performed firstly and afforded eleven potential bioactive compounds. The HPLC fingerprints of 19 batches of SC samples were evaluated by the chemometric methods such as similarity analysis (SA) and hierarchical clustering analysis (HCA). Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.047DOI Listing
January 2019
1 Read

Determination of volatile organic compounds in SW620 colorectal cancer cells and tumor-bearing mice.

J Pharm Biomed Anal 2019 Jan 30;167:30-37. Epub 2019 Jan 30.

Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address:

Early diagnosis and early treatment are important factors in reducing colorectal cancer (CRC) metastasis and mortality. Volatile organic compounds (VOCs) released by the human body have great potential for use in clinical diagnosis and therapeutic monitoring for CRC. The aim of our study was to identify VOCs with high specificity and high sensitivity for CRC and to provide a method for early diagnosis of CRC. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.050DOI Listing
January 2019
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A novel method using nuclear magnetic resonance for plasma protein binding assessment in drug discovery programs.

J Pharm Biomed Anal 2019 Jan 31;167:21-29. Epub 2019 Jan 31.

IRBM Science Park S.p.A., Via Pontina km 30.600, 00071, Pomezia, Rome, Italy. Electronic address:

A new methodology based on Nuclear Magnetic Resonance (NMR) was developed to determine plasma protein binding (PPB) of drug candidates in drug discovery programs. A strong correlation was found between the attenuation of NMR signals of diverse drugs in the presence of different plasma concentrations and their fraction bound (f) reported in the literature. Based on these results, a protocol for a rapid calculation of f of small molecules was established. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.049DOI Listing
January 2019

NADES-mediated folk plant extracts as novel antifungal agents against Candida albicans.

J Pharm Biomed Anal 2019 Jan 30;167:15-20. Epub 2019 Jan 30.

Instituto de Biología Agrícola de Mendoza (IBAM-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina.

Candida albicans is an opportunistic pathogenic yeast commonly found in mouth, gastrointestinal tract and vagina. Under certain conditions, it causes skin, mucosal and systemic infections. With growing concern over the emergence of resistant strains to conventional antifungals, the development of novel antifungal agents for the management of this pathogen is an urgent need. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.026DOI Listing
January 2019
1 Read

Analysis of unstable degradation impurities of a benzodiazepine and their quantification without isolation using multiple linear regression.

J Pharm Biomed Anal 2019 Jan 17;167:1-6. Epub 2019 Jan 17.

Pharma Research & Development, GlaxoSmithKline, Upper Providence, PA, USA.

This manuscript presents a novel methodology for calculating the relative response factors (RRFs) of unstable degradation impurities of molibresib (1). The degradation impurities were observed by HPLC during stress testing and were accompanied by large mass balance deficits. However, the impurities could not be isolated for traditional RRF determination due to their instability. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.028DOI Listing
January 2019
1 Read

Enantiomeric determination of α-lipoic acid in urine by LC/MS/MS.

J Pharm Biomed Anal 2019 Jan 28;166:435-439. Epub 2019 Jan 28.

Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-Ku, Tokyo, 142-8501, Japan. Electronic address:

An analytical method for the enantiomeric determination of α-lipoic acid in human urine was developed to evaluate the pharmacokinetics of α-lipoic acid, an ingredient in health food. Urine samples were collected over time after administering α-lipoic acid dietary supplement to healthy subjects. The samples were cleaned up by solid-phase extraction using an Oasis MAX cartridge. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.042DOI Listing
January 2019

Hydrophilic-interaction planar chromatography in ultra-sensitive determination of α-aminocephalosporin antibiotics. Application to analysis of cefalexin in goat milk samples using modified QuEChERS extraction technique.

J Pharm Biomed Anal 2019 Jan 3;166:421-434. Epub 2019 Jan 3.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.

A highly sensitive, selective and precise HPTLC method coupled with fluorescence detection was developed and validated for the determination of α-aminocephalosporin antibiotics; namely cefalexin, cefadroxil and cefradine in their standard solutions. The applicability of the developed methodology was demonstrated via analysis of cefalexin in goat milk samples. Full optimization of the fluorescence derivatization reaction was carried out with regard to the standard solutions of the studied compounds or after extraction of milk samples. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.001DOI Listing
January 2019

Determination of the species status of Fallopia multiflora, Fallopia multiflora var. angulata and Fallopia multiflora var. ciliinervis based on morphology, molecular phylogeny, and chemical analysis.

J Pharm Biomed Anal 2019 Jan 25;166:406-420. Epub 2019 Jan 25.

College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China. Electronic address:

Relationships among Fallopia multiflora (Thunb.) Haraldson., F. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.040DOI Listing
January 2019

Isotope dilution LC-orbitrap-HRMS with automated sample preparation for the simultaneous quantification of 11 antimycotics in human serum.

J Pharm Biomed Anal 2019 Jan 25;166:398-405. Epub 2019 Jan 25.

Institute of Laboratory Medicine, University Hospital, LMU Munich, Germany.

Introduction: The aim of this project was to develop and validate an isotope-dilution liquid chromatography high resolution mass spectrometry (LC-HRMS) method for the quantification of the 11 most widely used systemic antimycotics and to study whether HRMS is a feasible alternative for therapeutic drug monitoring (TDM) when compared to tandem MS (MS/MS) technology.

Methods: After protein precipitation, followed by automated online sample clean-up the analytes were separated within 4 min on a C18 column using an acetonitrile-water gradient. Eleven antimycotics, namely 5-flucytosine, amphotericin B, anidulafungin, fluconazole, isavuconazole, itraconazole, ketoconazole, micafungin, OH-itraconazole, posaconazole and voriconazole were finally quantified in full MS scan mode using positive electrospray ionization (ESI +) with a mass range fromm/z 110-1300 using HRMS. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.038DOI Listing
January 2019

Simultaneous quantitation of 14 DNA alkylation adducts in human liver and kidney cells by UHPLC-MS/MS: Application to profiling DNA adducts of genotoxic reagents.

J Pharm Biomed Anal 2019 Jan 25;166:387-397. Epub 2019 Jan 25.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address:

A rapid, sensitive and wide coverage ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method has been developed and validated for the simultaneous quantitation of 14 alkylation DNA adducts in cell genomic DNA, RNA and cell contents isolated from the in vitro cultured human kidney cell line 293 T and the human liver cell line L02 exposed to 3 genotoxic reagents: N-methyl-N-nitrosourea (MNU), methyl methanesulfonate (MMS) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). After exposure, DNA was isolated and directly hydrolysed under acid conditions or digested by enzymes to obtain the hydrolysates containing DNA alkylation adducts followed by optimization of the pretreatment method and chromatographic separation conditions. Quantification was performed on a Waters ACQUITY UPLC BEH Amide column (1. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.034DOI Listing
January 2019
2 Reads

Hypercrosslinked strong anion-exchange polymers for selective extraction of fluoroquinolones in milk samples.

J Pharm Biomed Anal 2018 Dec 31;166:379-386. Epub 2018 Dec 31.

Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangdong 510006, China. Electronic address:

In this work, hypercrosslinked strong anion-exchange polymer resins (HXLPP-SAX) were synthesized and evaluated as solid-phase extraction (SPE) sorbents for extracting fluoroquinolones (FQs) in milk samples. These particles were prepared using a protocol that was facile and cost-effective by combining hypercrosslinking reactions and quaternisation reactions in one step. After the synthesis, the morphological properties and anion-exchange, adsorption, and selectivity performances of the HXLPP-SAX resins were characterized and evaluated. Read More

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http://dx.doi.org/10.1016/j.jpba.2018.12.047DOI Listing
December 2018

Electrospun nanofiber-based niflumic acid capsules with superior physicochemical properties.

J Pharm Biomed Anal 2019 Jan 23;166:371-378. Epub 2019 Jan 23.

Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Interdisciplinary Excellence Centre, Eötvös Street 6, H-6720, Szeged, Hungary.

The aim of this study was to assess whether nanofibrous drug mats have potential as delivery systems for poorly water-soluble drugs. Amorphous nanofiber mats from a model poorly water-soluble active pharmaceutical ingredient (API), niflumic acid, together with the polymer excipient, polyvinyl pyrrolidine, were prepared by nozzle-free electrospinning. This technique offers a scalable way for drug formulation, and by increasing the surface area of the drug, the dissolution rate and therefore bioavailability of the API can be improved. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.037DOI Listing
January 2019
2 Reads

Identification and determination of bioactive phenylpropanoid glycosides of Aloysia polystachya (Griseb. et Moldenke) by HPLC-MS.

J Pharm Biomed Anal 2019 Jan 22;166:364-370. Epub 2019 Jan 22.

Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, Italy. Electronic address:

Aloysia polystachya (Griseb. et Moldenke) has not been deeply investigated in past years and currently data about its chemical composition are limited. Phenolic compounds characterization can be very difficult in vegetable matrices, owing to bonds to sugar moieties or conjugation, giving rise to complex structures. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.033DOI Listing
January 2019
1 Read

Identification of novel serum biomarker for the detection of acute myeloid leukemia based on liquid chromatography-mass spectrometry.

J Pharm Biomed Anal 2019 Jan 14;166:357-363. Epub 2019 Jan 14.

Department of Hematology, The Central Hospital of Xuhui District, Shanghai, 20031, China. Electronic address:

Acute myeloid leukemia (AML) is a life-threatening hematological malignancy. Traditional diagnosis of AML depends on invasive bone marrow biopsies. To recognize the metabolic characteristics related with AML and search for early non-invasive biomarkers of AML, in this work we applied ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOFMS)-based metabolomoc method to profile serum metabolites from 55 de novo AML patients and 45 age- and gender-matched healthy subjects and to screen and validate AML biomarkers. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.022DOI Listing
January 2019

A metabolomic study based on accurate mass and isotopic fine structures by dual mode combined-FT-ICR-MS to explore the effects of Rhodiola crenulata extract on Alzheimer disease in rats.

J Pharm Biomed Anal 2019 Jan 14;166:347-356. Epub 2019 Jan 14.

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China. Electronic address:

A metabolomic strategy based on accurate mass and isotopic fine structures (IFSs) by dual mode combined-Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was established to explore the effects of Rhodiola crenulata extract (RCE) on Alzheimer disease (AD) in rats. Experimental AD model was induced in rats by bilateral hippocampal injection of Aβ, and Morris water maze task (MWM) was used to evaluate the effects of RCE on AD. Subsequently, the metabolomic study was performed using HPLC-FT-ICR-MS, fraction collector and direct infusion (DI)-FT-ICR-MS to screen and identify the potential biomarkers. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.021DOI Listing
January 2019

Metabolomics profiling provides valuable insights into the underlying mechanisms of Morinda officinalis on protecting glucocorticoid-induced osteoporosis.

J Pharm Biomed Anal 2019 Jan 14;166:336-346. Epub 2019 Jan 14.

Department of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, China; Zhejiang Chinese Medical University School of Pharmacy, Hangzhou 310053, China. Electronic address:

Morinda officinalis (MO) has long been used as a traditional herbal medicine for the treatment of bone fractures and joint diseases in China. Monotropein (Mon) and rubiadin-1-methyl ether (Rub) are major bioactive components in MO. Ample evidence shows that MO and its chemical constituents can prevent osteoporosis induced by estrogen-deficiency and ageing. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.019DOI Listing
January 2019
2 Reads

The effect of fatty acids in red blood cell membranes on the dynamics of inflammatory markers following the coronary stent implantation.

J Pharm Biomed Anal 2019 Jan 11;166:310-325. Epub 2019 Jan 11.

Department of Biological and Biochemical Sciences, University of Pardubice, 532 10 Pardubice, Czech Republic.

The effect of 20 fatty acids in erythrocyte cell membranes on the extent of inflammatory response and cell oxidative stress was evaluated using multidimensional statistical data analysis in 54 patients suffering from ischemic heart disease undergoing percutaneous coronary intervention with coronary stent implantation using multidimensional statistical data analysis. A systemic inflammatory response was indicated by an increase of C-reactive protein (CRP), serum amyloid A (SAA) and ceruloplasmin 48 h after stent implantation and by an increase of interleukin-6 (IL-6) 24 h after intervention. The increase of malondialdehyde (MDA) after 48 h was used as a marker of cell damage by oxidative stress. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.002DOI Listing
January 2019

Analytical quality by design: Development and control strategy for a LC method to evaluate the cannabinoids content in cannabis olive oil extracts.

J Pharm Biomed Anal 2019 Jan 21;166:326-335. Epub 2019 Jan 21.

University of Florence, Department of Chemistry "U. Schiff", Via U. Schiff 6, 50019 Sesto Fiorentino, Florence, Italy. Electronic address:

Cannabidiol (CBD) and Δ-tetrahydrocannabinol (Δ-THC) are considered as the most interesting cannabinoids in Cannabis sativa L. for the clinical practice. Since 2013, the Italian law allows pharmacists to prepare and dispense cannabis extracts to patients under medical prescription, and requires the evaluation of CBD and Δ-THC content in cannabis extracts before sale. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.032DOI Listing
January 2019
6 Reads

Comparison between counterfeit and authentic medicines: A novel approach using differential scanning calorimetry and hierarchical cluster analysis.

J Pharm Biomed Anal 2019 Jan 17;166:304-309. Epub 2019 Jan 17.

Brazilian Federal Police of Rio Grande do Sul, 90160-093, Porto Alegre, RS, Brazil; Instituto Nacional de Ciência e Tecnologia Forense (INCT Forense), Porto Alegre, RS, Brazil. Electronic address:

Erectile dysfunction medicines such as Cialis and Viagra are very popular worldwide and are between the most prevalent counterfeit medicines in Brazil. A range of analytical methods has been used to analyze Cialis and Viagra, such as ATR-FTIR, GCMS and UPLC-MS. Until now, there are no data available of DSC methods for analysis of counterfeit medicines of Cialis and Viagra. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.029DOI Listing
January 2019
1 Read

Bioassay based screening for the antiplatelet aggregation quality markers of Polygonum multiflorum with UPLC and chemometrics.

J Pharm Biomed Anal 2019 Jan 9;166:264-272. Epub 2019 Jan 9.

China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing, China. Electronic address:

Currently, an increasing number of patients are seriously affected by acute thrombotic events. In China, Polygonum multiflorum (PM) is commonly used to treat diseases associated with thrombosis. Our previous work showed that PM could inhibit the platelet aggregation that plays a key role in the pathogenesis of thrombosis. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.005DOI Listing
January 2019
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High-performance countercurrent chromatographic isolation of acylated iridoid diglycosides from Verbascum ovalifolium Donn ex Sims and evaluation of their inhibitory potential on IL-8 and TNF-α production.

J Pharm Biomed Anal 2019 Jan 18;166:295-303. Epub 2019 Jan 18.

Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland.

In traditional folk medicine, Verbascum species have been used since ancient times to treat respiratory disorders, hemorrhoids, diarrhea, wounds, eczema and other types of inflammatory skin conditions. Despite the fact that more than 200 bioactive constituents (phenylethanoids, flavonoids, neolignan glycosides, phenolic acids, iridoids, saponins and polysaccharides) have been previously isolated from various Verbascum species, to date preparative high-performance countercurrent chromatography (HPCCC) has never been employed for this purpose. Therefore, in this study, simple HPCCC methods were successfully developed with the aim to primarily isolate acylated iridoid diglycosides from Verbascum ovalifolium Donn ex Sims (oval-leaved mullein). Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.031DOI Listing
January 2019
2 Reads

HPLC-MS/MS analysis of ilimaquinone and its application in a pharmacokinetic study in rats.

J Pharm Biomed Anal 2019 Jan 17;166:291-294. Epub 2019 Jan 17.

College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea. Electronic address:

Ilimaquinone, a metabolite isolated from the marine sponge Hippiospongia metachromia, has antimicrobial, cytotoxic, anti-HIV, anti-inflammatory, and anti-cancer activities. A new quantitative analytical method for determination of ilimaquinone in rat plasma using HPLC-MS/MS was developed and validated. Ascorbic acid was added to ensure the stability of ilimaquinone in plasma. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.030DOI Listing
January 2019
3 Reads

Rapid characterization of the absorbed constituents in rat serum after oral administration and action mechanism of Naozhenning granule using LC-MS and network pharmacology.

J Pharm Biomed Anal 2019 Jan 14;166:281-290. Epub 2019 Jan 14.

Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China. Electronic address:

Naozhenning granule is a Chinese herbal formula, which is mainly used in the treatment of concussion, cerebral post-traumatic syndrome. Although its effectiveness has been certified in the clinical use, the mechanism of action and bioactive components of Naozhenning remain unknown. In this study, the ultrahigh performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry was applied to identify the absorbed constituents of Naozhenning in the rat serum. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.020DOI Listing
January 2019
2 Reads

Characterization of tolazamide binding with glycated and normal human serum albumin by using high-performance affinity chromatography.

J Pharm Biomed Anal 2019 Jan 14;166:273-280. Epub 2019 Jan 14.

Chemistry Department, University of Nebraska-Lincoln, Lincoln, NE 68588, USA. Electronic address:

Sulfonylurea drugs are antidiabetic drugs that are utilized in the treatment of type II diabetes and often have significant binding with human serum albumin (HSA). Immobilized samples of normal or glycated HSA in affinity microcolumns were used to investigate interactions of these proteins with the sulfonylurea drug tolazamide. HPLC and frontal analysis were used to first examine the overall binding of this drug with these samples of HSA. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.025DOI Listing
January 2019
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Determination of residues and metabolites of more than 140 pharmacologically active substances in meat by liquid chromatography coupled to high resolution Orbitrap mass spectrometry.

J Pharm Biomed Anal 2019 Jan 14;166:252-263. Epub 2019 Jan 14.

Institute of Food Safety, Animal Health and Environment "BIOR", Lejupes iela 3, Riga LV-1076, Latvia.

This study reports an analytical method for simultaneous identification, screening and quantification of 164 residues and metabolites of pharmacologically active substances belonging to such therapeutic classes as anti-infectious (antibiotics and chemotheurapeutics), anti-inflammatory and antiparasitic agents (against protozoa, endo- and ectoparasites), corticoids and agents acting on the nervous and reproductive systems, substances with hormonal and thyreostatic action, and beta agonists. Different sample preparation procedures were compared and optimised for the detection of selected veterinary drugs in chicken, porcine and bovine meat by ultra-high performance liquid chromatography coupled to high-resolution Orbitrap mass spectrometry. The optimised instrumental method and sample preparation procedures were validated by fortifying blank matrix at four levels (0. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.024DOI Listing
January 2019
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Development and validation of a UPLC-MS/MS method for determination of motesanib in plasma: Application to metabolic stability and pharmacokinetic studies in rats.

J Pharm Biomed Anal 2019 Jan 14;166:244-251. Epub 2019 Jan 14.

Pharmacology and Toxicology Department, Faculty of Pharmacy (Girls) Al Azhar University, Cairo, Egypt.

Motesanib is a potent angiokinase inhibitor, has shown potential therapeutic effects against various cancers. An accurate, reproducible, rapid, specific, sensitive, and valid ultraperformance liquid chromatography-tandem mass spectrometry method was established to quantify motesanib in rat plasma. Motesanib and linifanib (used as an internal standard; IS) were extracted from plasma by liquid-liquid extraction using tert-butyl methyl ether as extracting agent. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.023DOI Listing
January 2019
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Rapid, hydrolysis-free, dilute-and-shoot method for the determination of buprenorphine, norbuprenorphine and their glucuronides in urine samples using UHPLC-MS/MS.

J Pharm Biomed Anal 2019 Jan 11;166:236-243. Epub 2019 Jan 11.

Università di Urbino Carlo Bo, DISPEA, Piazza Rinascimento 6, 61029 Urbino, Italy.

Buprenorphine and its metabolites are routinely monitored to assess patient compliance with drug detoxification programs or as pain killers. A rapid method for the simultaneous analysis of buprenorphine, norbuprenorphine, and glucuronides in urine using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. Urine samples were diluted in water containing formic acid 0. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.014DOI Listing
January 2019

A systematic review of the stability of finished pharmaceutical products and drug substances beyond their labeled expiry dates.

J Pharm Biomed Anal 2019 Jan 11;166:222-235. Epub 2019 Jan 11.

University of Würzburg, Institute for Pharmacy and Food Chemistry, Am Hubland, 97074 Würzburg, Germany. Electronic address:

In recent years, there has been a very active debate about the stability of drug products especially after exceeding the expiry dates. The regulatory authorities require comprehensive stability data for market approval. The shelf-life obtained determines the expiry date, which is typically between 1 and 5 years and commonly set in a conservative manner. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.016DOI Listing
January 2019

Ultrasound-assisted emulsification liquid phase microextraction method based on deep eutectic solvent as extraction solvent for determination of five pesticides in traditional Chinese medicine.

J Pharm Biomed Anal 2019 Jan 14;166:213-221. Epub 2019 Jan 14.

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, 110016, Shenyang, Liaoning Province, PR China; School of Pharmacy, Huaihai Institute of Technology, Lianyungang, Jiangsu 222001, China. Electronic address:

A kind of green solvents termed deep eutectic solvent (DES) have been considered alternatives to traditional organic solvents. In this study, a method was developed for determination of five pesticide residues including triadimenol, fipronil, tebuconazole, hexaconazole and diniconazole in five different traditional Chinese medicines (TCMs). In the developed method, DES was selected as extraction solvent, ultrasound-assisted emulsification liquid phase microextraction (UA-ELPME) was used for extraction and high performance liquid chromatography (HPLC) equipped with a diode array detector (DAD) was used for quantification. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.018DOI Listing
January 2019

Sub-picomolar quantification of PTH 1-34 in plasma by UHPLC-MS/MS after subcutaneous injection of teriparatide and identification of PTH 1-33, its degradation product.

J Pharm Biomed Anal 2019 Jan 9;166:205-212. Epub 2019 Jan 9.

Service of Clinical Pharmacology, Lausanne University Hospital (CHUV), Lausanne, Switzerland.

Teriparatide (PTH 1-34, Forsteo) is a bioactive N-terminal fragment of the native endogenous parathyroid hormone (PTH 1-84) recommended for the treatment of osteoporosis in patients with high risk of fracture. Since PTH 1-34 may undergo proteolysis we have validated an ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for unambiguously measuring intact PTH 1-34 with the same sensitivity as ELISA, at subpicomolar level (LLOQ at 0.4 pM). Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.011DOI Listing
January 2019
3 Reads

Determination of total and unbound ribociclib in human plasma and brain tumor tissues using liquid chromatography coupled with tandem mass spectrometry.

J Pharm Biomed Anal 2019 Jan 11;166:197-204. Epub 2019 Jan 11.

Karmanos Cancer Institute, Wayne State University, Detroit, MI, 48201, United States. Electronic address:

Ribociclib is a selective, orally bioavailable inhibitor of cyclin-dependent kinase (CDK) 4/6, which has therapeutic potential for a variety of cancer types. This study was to develop and validate a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for determining total and unbound concentrations of ribociclib in human plasma and brain tumor tissue samples. Plasma and tumor homogenate samples were extracted using protein precipitation with acetonitrile. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.017DOI Listing
January 2019
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A strategy based on fingerprinting and chemometrics for the detection of regulated plants in plant food supplements from the Belgian market: Two case studies.

J Pharm Biomed Anal 2019 Jan 11;166:189-196. Epub 2019 Jan 11.

Scientific Direction Chemical and Physical Health Risks, Section Medicines & Health Products, Sciensano, J. Wytsmanstraat 14, B-1050, Brussels, Belgium.

The sale and consumption of plant food supplements is increasing, especially in the western world. A lot of these supplements can be bought through internet, where a lot of illegal trade is going on. Every year seized dietary supplements are send to laboratories in order to screen for the presence of chemical adulterants or illegally added active pharmaceutical ingredients, though also herbal adulteration occurs and is given less attention. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183276
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http://dx.doi.org/10.1016/j.jpba.2019.01.015DOI Listing
January 2019
9 Reads

A validated UPLC-MS/MS method for pharmacokinetic study of inflachromene, a novel microglia inhibitor.

J Pharm Biomed Anal 2019 Jan 9;166:183-188. Epub 2019 Jan 9.

Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea. Electronic address:

Inflachromene (ICM), a novel microglia inhibitor, is under development to treat neuroinflammatory disorders. For the pharmacokinetic evaluation of ICM, we developed quantitative ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method in rat plasma. Protein precipitation method with acetonitrile (ACN) was used for plasma sample preparation. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.013DOI Listing
January 2019
1 Read
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