12,666 results match your criteria Journal of pharmaceutical and biomedical analysis[Journal]


Degradation studies of Ibrutinib under stress conditions: Characterisation and structural elucidation of novel degradants.

J Pharm Biomed Anal 2019 Apr 12;172:9-17. Epub 2019 Apr 12.

Department of Engineering Chemistry, Andhra University, Visakhapatnam, A.P., 530003, India.

The aim of the research work is to study the degradation behaviour of Ibrutinib (IBN) which is performed under different stress conditions according to International Conference on Harmonization guidelines (ICH). The study included monitoring degradation of the Ibrutinib drug under acidic, base, oxidation, thermal and photolytic conditions followed by isolation and characterisation of degradation products (DP) by Liquid Chromatography Mass Spectrometry (LCMS), High resolution Mass Spectrometry (HR-MS/MS) and Nuclear Magnetic Resonance (NMR) studies. The IBN drug is stable under oxidation, thermal and photolytic conditions. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.010DOI Listing

Pharmaceutical and preclinical evaluation of Advax adjuvant as a dose-sparing strategy for ant venom immunotherapy.

J Pharm Biomed Anal 2019 Apr 9;172:1-8. Epub 2019 Apr 9.

Jack Jumper Allergy Program, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia; School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, South Australia, 5001, Australia.

A major challenge in broader clinical application of Jack Jumper ant venom immunotherapy (JJA VIT) is the scarcity of ant venom which needs to be manually harvested from wild ants. Adjuvants are commonly used for antigen sparing in other vaccines, and thereby could potentially have major benefits to extend JJA supplies if they were to similarly enhance JJA VIT immunogenicity. The purpose of this study was to evaluate the physicochemical and microbiological stability and murine immunogenicity of low-dose JJA VIT formulated with a novel polysaccharide adjuvant referred to as delta inulin or Advax™. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.017DOI Listing

Speciation analysis of arsenic in urine samples from APL patients treated with single agent AsO by HPLC-HG-AFS.

J Pharm Biomed Anal 2019 Apr 8;171:212-217. Epub 2019 Apr 8.

Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address:

Arsenic trioxide [AsO, arsenite (As) in solution] has been applied successfully for the treatment of acute promyelocytic leukemia (APL). The arsenic speciation analysis of urine is critical to reveal the metabolic mechanism and the relationship between arsenic species and the clinical response. To characterize the arsenic species in urine, a simple and robust HPLC-HG-AFS method was developed and validated to quantify the levels of arsenic species [As and its metabolites, monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), and arsenate (As)] in urine samples from 66 patients with APL. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.014DOI Listing

Development and validation of a multiplexed drug level assay in support of combination biologics therapy clinical studies.

J Pharm Biomed Anal 2019 Apr 13;171:204-211. Epub 2019 Apr 13.

Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Inc., 2000 Galloping Hill Rd, Kenilworth, NJ 07033, USA.

Clinical development of biotherapeutics for combination therapy requires monitoring the concentrations of both drugs in biological samples. Traditionally, two assays are required to measure drug levels one at a time, which poses challenges in sample management, data reporting, and cost. The Meso Scale Discovery (MSD®) U-PLEX platform provides a simple and flexible way to create custom multiplex ligand binding assays (LBAs). Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.021DOI Listing

Multi-class determination of steroid hormones and antibiotics in fatty hotpot ingredients by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2019 Apr 15;171:193-203. Epub 2019 Apr 15.

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, 110016, Shenyang, Liaoning Province, PR China. Electronic address:

Due to the misuse of hormones and antibiotics resulting in drug residues in meat products, serious concerns have arisen regarding to the potential risks for human health. In this paper, a simple and efficient method was developed to simultaneously quantify six steroid hormones and six antibiotics in fatty foods by combining pressurized liquid extraction (PLE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Adsorbents, extraction temperature, extraction time, recycle time and several other parameters were optimized in current investigation. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.019DOI Listing

A systemic combined nontargeted and targeted LC-MS based metabolomic strategy of plasma and liver on pathology exploration of alpha-naphthylisothiocyanate induced cholestatic liver injury in mice.

J Pharm Biomed Anal 2019 Apr 8;171:180-192. Epub 2019 Apr 8.

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China. Electronic address:

The pathology of cholestatic liver injury (CLI) was complicated, which has limited the development of anti-cholestatic drugs for a long period. Metabolomic researches focused on global and dynamic changes of the organism could shed some light on mechanism investigation. In order to characterize and validate metabolite alterations of alpha-naphthylisothiocyanate (ANIT) induced CLI in C57BL/6 mice, a systemic metabolomic approach combining nontargeted HPLC-ESI-QTOF-MS and targeted UFLC-ESI-MS/MS technologies were developed innovatively. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.009DOI Listing
April 2019
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An optimized analytical method for cellular targeted quantification of primary metabolites in tricarboxylic acid cycle and glycolysis using gas chromatography-tandem mass spectrometry and its application in three kinds of hepatic cell lines.

J Pharm Biomed Anal 2019 Apr 12;171:171-179. Epub 2019 Apr 12.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.

Energy synthesis in aerobic organisms relies on two major metabolic pathways, i.e. tricarboxylic acid (TCA) cycle and glycolysis, the metabolites of which are highly affected by many diseases. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085193009
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http://dx.doi.org/10.1016/j.jpba.2019.04.022DOI Listing
April 2019
1 Read

Reply to "Requirements for accurate H NMR quantification of mineral oil hydrocarbons (paraffins) for pharmaceutical or cosmetic use".

J Pharm Biomed Anal 2019 Apr 10. Epub 2019 Apr 10.

University of Würzburg, Institute for Pharmacy and Food Chemistry, 97074, Würzburg, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.jpba.2019.04.020DOI Listing

Comparison of isotope pattern deconvolution and calibration curve quantification methods for the determination of estrone and 17β-estradiol in human serum.

J Pharm Biomed Anal 2019 Apr 9;171:164-170. Epub 2019 Apr 9.

Department of Clinical Chemistry, University of Liège, CHU Sart-Tilman, Liège, Belgium. Electronic address:

A Liquid Chromatography coupled to tandem mass spectrometry (LC-MS/MS) based method have been developed for the determination of the main estrogen compounds -estrone (E1) and 17β-estradiol (E2)- in human serum. Two isotope dilution mass spectrometry (IDMS) quantification procedures have been used: a classical calibration curve-based method were compared to a recently developed isotope pattern deconvolution (IPD) method. IPD is based on isotopic abundance measurements and multiple linear regression. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085193032
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http://dx.doi.org/10.1016/j.jpba.2019.04.013DOI Listing
April 2019
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Rapid quantitative analysis of ginkgo flavonoids using paper spray mass spectrometry.

J Pharm Biomed Anal 2019 Apr 10;171:158-163. Epub 2019 Apr 10.

Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.

In this work, a method for the rapid and reliable analysis of ginkgo flavonoids was developed by using paper spray mass spectrometry (PS-MS) assisted by microwave-assisted hydrolysis (MAH). Kaempferol-D, quercetin-D, and isorhamnetin-D were used as internal standards (IS). Samples analyses were completed by simply treating with extraction, MAH, dilution, and quantitative analysis by PS-MS. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.018DOI Listing
April 2019
4 Reads

Lysophospholipid profiles of apolipoprotein E-deficient mice reveal potential lipid biomarkers associated with atherosclerosis progression using validated UPLC-QTRAP-MS/MS-based lipidomics approach.

J Pharm Biomed Anal 2019 Apr 5;171:148-157. Epub 2019 Apr 5.

Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Lysophospholipids (Lyso-PLs) are lipid-derived signaling molecules which were demonstrated to have a strong correlation with the progression of atherosclerosis. In this study, we investigated the influence of high-fat diet on Lyso-PL profiles of atherosclerosis-prone apolipoprotein E-deficient (ApoE) mice and wild type C57BL/6 J mice to find out the potential biomarkers associated with atherosclerosis. Firstly, the quantitative profiling method for Lyso-PLs based on ultra-performance liquid chromatography-quadrupole linear ion trap mass spectrometry (UPLC-QTRAP-MS/MS) was established and validated. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.062DOI Listing
April 2019
1 Read

Belizatinib: Novel reactive intermediates and bioactivation pathways characterized by LC-MS/MS.

J Pharm Biomed Anal 2019 Apr 6;171:132-147. Epub 2019 Apr 6.

Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt. Electronic address:

Belizatinib (BZB; TSR-011) is a next-generation anaplastic lymphoma kinase inhibitor that also inhibits tropomyosin-related kinases A/B/C. In this in-vitro study, we examined the formation of reactive metabolites from BZB using rat liver microsomes or human liver microsomes in the presence of a trapping agent (potassium cyanide) to generate iminium reactive intermediates. Identification of the in vitro BZB metabolites indicated that the major in-vitro metabolic reaction involved hydroxylation of the piperidine moiety. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085193001
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http://dx.doi.org/10.1016/j.jpba.2019.04.006DOI Listing
April 2019
2 Reads

Determination of fascaplysin in rat plasma with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS): application to a pharmacokinetic study.

J Pharm Biomed Anal 2019 Apr 8;171:126-131. Epub 2019 Apr 8.

Zhejiang Province Key Laboratory of Anesthesiology, Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address:

A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to determine fascaplysin in rat plasma using methylfascaplysin as an internal standard. Both fascaplysin and methylfascaplysin were synthesized in our laboratory. Protein precipitation was applied for the preparation of the plasma sample. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085193019
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http://dx.doi.org/10.1016/j.jpba.2019.04.012DOI Listing
April 2019
3 Reads

Chemical fingerprints, antioxidant, enzyme inhibitory, and cell assays of three extracts obtained from Sideritis ozturkii Aytaç & Aksoy: An endemic plant from Turkey.

J Pharm Biomed Anal 2019 Apr 8;171:118-125. Epub 2019 Apr 8.

University of Mauritius, Faculty of Science, Department of Health Sciences, Réduit, Mauritius.

This study was geared towards assessing the possible antioxidant, enzyme inhibitory, and cytotoxic activities of ethyl acetate, methanol, and water extracts of Sideritis ozturkii Aytaç & Aksoy. The phytochemical profiles of the studied extracts were characterised by HPLC-MS/MS. The methanol extract, rich in phenolics (78. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085193029
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http://dx.doi.org/10.1016/j.jpba.2019.04.011DOI Listing
April 2019
2 Reads

A sensitive and selective multiple reaction monitoring mass spectrometry method for simultaneous quantification of flavonol glycoside, terpene lactones, and biflavonoids in Ginkgo biloba leaves.

J Pharm Biomed Anal 2019 Mar 27;170:335-340. Epub 2019 Mar 27.

The College of Forestry, Beijing Forestry University, 100083, Beijing, PR China; Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083, Beijing, PR China; Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040, Harbin, PR China. Electronic address:

In this study, an efficient and sensitive UHPLC-QQQ-MS/MS (MRM) analytical strategy was established firstly for simultaneous determination of 11 components, including 3 original flavonol glycoside, 4 terpene lactones and 4 biflavonoids in Ginkgo biloba leaves. The validated strategy exhibited proper linearity (R ≥0.99) in the range of 0. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.058DOI Listing
March 2019
1 Read

Development and validation of a simple, fast, and sensitive LC/MS/MS method for the quantification of oxfendazole in human plasma and its application to clinical pharmacokinetic study.

J Pharm Biomed Anal 2019 Mar 26;171:111-117. Epub 2019 Mar 26.

Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, IA, 52242, USA. Electronic address:

The most popular standard treatments for soil transmitted helminths in humans including mebendazole, albendazole, levamisole, and pyrantel pamoate, show greatly variable efficacy against different species of parasites and have unfavorable pharmacokinetic characteristics, such as short half-life. The transition of oxfendazole, a potent broad-spectrum anthelmintic with long half-life, from veterinary medicine to human use has been considered as a promising approach. However, analytical methods for the quantitative detection of oxfendazole in human matrix are very limited and lack sensitivity. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.048DOI Listing
March 2019
1 Read

Requirements for accurate H NMR quantification of mineral oil hydrocarbons (paraffins) for pharmaceutical or cosmetic use.

J Pharm Biomed Anal 2019 Apr 1. Epub 2019 Apr 1.

Chemisches und Veterinäruntersuchungsamt (CVUA) Karlsruhe, Weissenburger Strasse 3, 76187 Karlsruhe, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.jpba.2019.03.059DOI Listing
April 2019
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Simultaneous quantification of tegoprazan and its major metabolite M1 in dog plasma using liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2019 Mar 21;171:99-103. Epub 2019 Mar 21.

CJ Healthcare Corporation, Deokpyeong-ro 811, Majang-myeon, Icheon-si, Gyeonggi-do, 17389, Republic of Korea. Electronic address:

Tegoprazan is a novel potassium-competitive acid blocker (P-CAB) recently approved in Korea as a next-generation therapeutics for gastric acid-related diseases. In the present study, we demonstrate a simple bioanalytical liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of tegoprazan and its major metabolite (M1) in dog plasma. The developed method is based on protein precipitation and LC-MS/MS, validated according to the regulatory guidance for bioanalytical method validation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183293
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http://dx.doi.org/10.1016/j.jpba.2019.03.045DOI Listing
March 2019
2 Reads

In vivo metabolic profiles of Bu-Zhong-Yi-Qi-Tang, a famous traditional Chinese medicine prescription, in rats by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.

J Pharm Biomed Anal 2019 Apr 3;171:81-98. Epub 2019 Apr 3.

College of Pharmacy, Jinan University, Guangzhou 510632, PR China; Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, PR China; College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China. Electronic address:

Bu-Zhong-Yi-Qi-Tang (BZYQT), a famous traditional Chinese medicine prescription (TCMP), has been extensively used for conditioning sub-health status and diseases caused by spleen-qi deficiency in China for over 700 years. BZYQT is prevalent not only in China, but also in Japan and South Korea for the clinical treatment of chronic diseases, such as fatigue, tuberculosis and loss of appetite after surgery. However, due to a lack of research on the holistic metabolism of BZYQT, the in vivo bioactive components of BZYQT remain unclear, hindering further study of its in vivo mechanism of action and quality control. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.001DOI Listing

A clinical study for the evaluation of pharmacokinetic interaction between daclatasvir and fluoxetine.

J Pharm Biomed Anal 2019 Apr 5;171:104-110. Epub 2019 Apr 5.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut 71526, Egypt; Current address: Schulich School of Engineering, University of Calgary, Calgary, AB T2N 4V8, Canada. Electronic address:

A simple and sensitive chromatographic method has been developed for the quantitative analysis of an antiviral agent, daclatasvir (DCV), that commonly prescribed for the treatment of hepatitis C viral (HCV) infection. The method was applied to detect DCV in human plasma and real blood samples collected from patients diagnosed with HCV and treated with DCV. The analysis strategy was based on recording the native fluorescence of DCV in plasma, after pre-column treatment to precipitate the plasma proteins using a readily applicable protocol. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183292
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http://dx.doi.org/10.1016/j.jpba.2019.03.065DOI Listing
April 2019
4 Reads

A study on structural characterization of degradation products of cangrelor using LC/QTOF/MS/MS and NMR.

J Pharm Biomed Anal 2019 Mar 28;170:327-334. Epub 2019 Mar 28.

Centre for Chemical Science & Technology, Institute of Science & Technology, JNTUH, Kukatpally, Hyderabad, 500 085, India.

A complete degradation study was performed on cangrelor drug substance as per the ICH guidelines. The study reveals that a total of six degradation products (DP-1 to DP-6) were found and out of these, three unknown degradation products (DP-1, DP-5 and DP-6) were not reported in the literature. Based on the degradation study, the drug substance cangrelor was found to be sensitive towards acidic, basic and oxidative conditions. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.031DOI Listing
March 2019
4 Reads

Measurement uncertainty and risk of false conformity decision in the performance evaluation of liquid chromatography analytical procedures.

J Pharm Biomed Anal 2019 Apr 4;171:73-80. Epub 2019 Apr 4.

Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 13, CEP 05508-000, São Paulo, SP, Brazil. Electronic address:

Liquid chromatography is one of the main techniques used in pharmaceutical quality control analytical procedures. However, there will always be a measurement uncertainty (MU) associated with them, that can lead to the approval of an out of specification lot (consumer risk) or rejection of a lot within specification (producer risk). Thus, the aim of this study was to evaluate the performance of liquid chromatography analytical procedures based on their measurement uncertainty and to estimate the risk of false conformity decisions. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.005DOI Listing
April 2019
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Simultaneous analysis of volatile and semi-volatile components in a topical formulation by gas chromatography using a programmed temperature vaporization inlet and flame ionization detection.

J Pharm Biomed Anal 2019 Apr 1;171:65-72. Epub 2019 Apr 1.

KU Leuven - University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, O&N2, Herestraat 49/923, B-3000 Leuven, Belgium. Electronic address:

Topical formulations are medications applied locally on the skin to treat ailment. They are made up of complex mixtures of active ingredients and excipients. Till date, no analytical method has been found in literature that is able to simultaneously analyze volatile and semi-volatile actives present in topical formulations. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.057DOI Listing
April 2019
2 Reads

Structural characterization and screening of chemical markers of flavonoids in Lysimachiae Herba and Desmodii Styracifolii Herba by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry based metabolomics approach.

J Pharm Biomed Anal 2019 Apr 3;171:52-64. Epub 2019 Apr 3.

Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, Hebei 050017, PR China. Electronic address:

In traditional Chinese medicine, Lysimachiae Herba (LH) and Desmodii Styracifolii Herba (DSH) have been widely used for the treatment of calculi, but there is a certain focus in clinical application. Flavonoids as their pharmacologically active substances were focusly studied to make clear of their chemical compositions and reveal the similarities and differences between LH and DHS by analysis of characteristic marker components at the molecular level. An ultra high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) approach based on metabolite profiling was established. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.003DOI Listing
April 2019
2 Reads

Pharmacokinetic comparisons of major bioactive components after oral administration of raw and steamed rhubarb by UPLC-MS/MS.

J Pharm Biomed Anal 2019 Apr 2;171:43-51. Epub 2019 Apr 2.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16 Nanxiao Lane, Dongzhimennei, Beijing, 100700, China. Electronic address:

A study was performed to compare the pharmacokinetics of major bioactive analytes in rhubarb plants to explore the pharmacological differences between raw pieces (RP) and steamed pieces (SP) of rhubarb. A rapid and efficient ultra-performance liquid chromatographic coupled with mass/mass spectrometry (UPLC-MS/MS) method was established to determine the concentrations of six analytes in rat plasma after oral administration of RP and SP. It was found that the AUC (area under the plasma concentration-time curve), T (time to reach maximum plasma concentration) and C (maximum plasma concentration) values were obviously increased in RP for rhein-8-O-β-d-glucophyranoside, rhein and aloe-emodin. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.002DOI Listing
April 2019
2 Reads
2.979 Impact Factor

Identification of eupatilin and ginkgolide B as p38 ligands from medicinal herbs by surface plasmon resonance biosensor-based active ingredients recognition system.

J Pharm Biomed Anal 2019 Mar 15;171:35-42. Epub 2019 Mar 15.

School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address:

Screening of bioactive ligands for a certain protein target from medicinal herbs is a highly important yet challenging task during drug discovery process. In this study, a surface plasmon resonance biosensor-based active ingredient recognition system (SPR-AIRS) was applied to screen p38 mitogen-activated protein kinase (p38) ligands from herbal extracts. After p38 protein was immobilized on a SPR chip and the suitability of SPR-AIRS was validated, thirty-four p38-related medicinal herbs were selected and pre-screened. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.029DOI Listing
March 2019
1 Read

An unexpected Griess reaction on the important anti-malarial drug primaquine and its application for drug determination.

J Pharm Biomed Anal 2019 Mar 30;171:8-14. Epub 2019 Mar 30.

Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 501405, China. Electronic address:

Primaquine (PMQ), a well-known anti-malarial drug, is of increasing importance as people moving toward global malaria eradication. PMQ has serious side effects that it often causes acute hemolytic toxicity in people with glucose-6-phosphate dehydrogenase (G6PD) deficiency. The development of simple and reliable approaches for quantitative dose monitoring is thus becoming important during malarial treatment with PMQ. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.063DOI Listing
March 2019
2 Reads
2.979 Impact Factor

Pharmacokinetics of 40 kDa PEG in rodents using high-field NMR spectroscopy.

J Pharm Biomed Anal 2019 Apr 1;171:30-34. Epub 2019 Apr 1.

Discovery Analytical Sciences, Bristol-Myers Squibb, Route 206 & Province Line Rd, Princeton NJ 08543, USA.

Conjugation of macromolecular drugs to polyethylene glycol (PEG) improves their therapeutic potential by reducing their rate of degradation, thereby extending the drugs half life. As a substantial component of the drug, it is necessary to measure the pharmacokinetic (PK) characteristics of PEG in vivo. A quantitative NMR-based method was developed and successfully applied to measuring double-branched polyethylene glycol 40 kDa (PEG40) in serum samples, enabling determination of PK parameters of PEG40 in preclinical species. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085193040
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http://dx.doi.org/10.1016/j.jpba.2019.03.066DOI Listing
April 2019
1 Read

Fatty acids-based microemulsion liquid chromatographic determination of multiple caffeoylquinic acid isomers and caffeic acid in honeysuckle sample.

J Pharm Biomed Anal 2019 Apr 1;171:22-29. Epub 2019 Apr 1.

College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address:

A green and efficient microemulsion liquid chromatographic (MELC) method using fatty acid as co-surfactant and electrochemical detection was established and validated for the determination of four caffeoylquinic acid isomers and caffeic acid in honeysuckle samples. The influences of each individual component within the isocratic oil-in-water (O/W) microemulsion mobile phase were systematically investigated, such as the type and concentration of co-surfactant, concentration of sodium dodecyl sulphate (SDS), organic modifier addition, type and concentration of oil phase, pH and detection voltage. Results indicated that excellent resolution was achieved using 3. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.064DOI Listing
April 2019
1 Read

LC-MS/MS study of the degradation processes of nitisinone and its by-products.

J Pharm Biomed Anal 2019 Mar 30;171:15-21. Epub 2019 Mar 30.

Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6 Str, 44-100 Gliwice, Poland.

Nitisinone (2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione, NTBC) was the first synthetically produced triketone herbicide. However, its unsatisfactory herbicidal properties, negative impact on the natural environment and the high cost of synthesis have hindered its commercialization as a plant protection agent. Nevertheless, NTBC has become the medical treatment of choice for a rare hereditary metabolic disease -hepatorenal tyrosinemia. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.046DOI Listing

Spectroscopic identification of benign (follicular adenoma) and cancerous lesions (follicular thyroid carcinoma) in thyroid tissues.

J Pharm Biomed Anal 2019 Mar 28;170:321-326. Epub 2019 Mar 28.

Center for Innovation and Transfer of Natural Sciences and Engineering Knowledge, University of Rzeszow, PL-35959, Rzeszow, Poland.

Thyroid follicular nodules are quite common in the population, however only a small proportion is malignant. Thyroid cancer differs from adenoma by features of cellular atypia, angioinvasiveness and possibility of metastasis via blood vessels mainly in the lungs and bones. Pathomorphological examination of the postoperative material plays a significant role in the diagnosis of cystic thyroid lesions. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.061DOI Listing
March 2019
3 Reads

Chemical and metabolic analysis of Achyranthes bidentate saponins with intestinal microflora-mediated biotransformation by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with metabolism platform.

J Pharm Biomed Anal 2019 Mar 28;170:305-320. Epub 2019 Mar 28.

Anhui University of Chinese Medicine, Hefei 230012, China; Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui Province Key Laboratory of R&D of Chinese Medicine, Hefei 230012, China; Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei 230012, China.

Achyranthes bidentate (AB) is a typical traditional Chinese medicine (TCM) that has been widely used in clinical practices for more than a thousand years. Modern pharmacological studies have shown that triterpene saponins are the main pharmacological active ingredients in AB. Meanwhile, the poor oral bioavailability of triterpene saponins in AB indicates that these ingredients are probably metabolized by intestinal microflora before absorption. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.041DOI Listing
March 2019
2 Reads
2.979 Impact Factor

Elimination of ghost peaks by optimization of anion exchange chromatography method for determination of gamma-carboxyglutamic acid (Gla)-domainless impurity in recombinant activated clotting factor VII drug products.

J Pharm Biomed Anal 2019 Mar 22;171:1-7. Epub 2019 Mar 22.

Biopharmaceutical Research Center, AryoGen Pharmed Inc., Alborz University of Medical Sciences, Karaj, Iran. Electronic address:

Secreted recombinant activated clotting factor VII activated (rFVIIa) in cell culture media missing gamma-carboxyglutamic acid (Gla) domain as a result of failure in gamma-carboxylation or cell lysis is called Gla-domainless impurity which has less negative charge compared to native rFVIIa. Based on risk assessment, this type of impurity is considered as critical drug product quality attribute of rFVIIa and its quantitative analysis in product batches is a critical issue in quality control laboratories. Analysis of Gla-domainless impurity is accomplished by Strong Anion Exchange Chromatography (SAX) in recombinant factor VIIa using Tris and Bis-Tris propane salt buffers as equilibrating buffers and high concentration ammonium acetate as an eluent. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.011DOI Listing
March 2019
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Qualitative analysis of the roots of Salvia miltiorrhiza and Salvia yunnanensis based on NIR, UHPLC and LC-MS-MS.

J Pharm Biomed Anal 2019 Jan 9;170:295-304. Epub 2019 Jan 9.

Bayer AG, Leverkusen, 51368, Germany.

Near infrared spectroscopy (NIR) was applied to discriminate the roots of salvia miltiorhiza Bunge (Danshen for short) and Salvia yunnanensis C. H. Wright (Zidanshen for short) by means of principal component analysis (PCA), improved and simplified K nearest neighbors (IS-KNN). Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.010DOI Listing
January 2019
2 Reads
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Efficient extraction of flavonoids from Flos Sophorae Immaturus by tailored and sustainable deep eutectic solvent as green extraction media.

J Pharm Biomed Anal 2018 Dec 20;170:285-294. Epub 2018 Dec 20.

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040, Harbin, PR China. Electronic address:

In the face of the many shortcomings of conventional organic solvents in the age of green chemistry, deep eutectic solvents (DESs) appear under the spotlight of natural product extraction because of its outstanding advantages. In this study, the extraction of six compounds from Flos Sophorae Immaturus (FSI) with DES-5 (choline chloride/1,4-butanediol) as its topgallant solvent was determined by screening nine DESs. After single factor test and BBD experiment, the optimum conditions of deep eutectic solvent-based microwave-assisted extraction (DES-MAE) were: choline chloride/1,4-butanediol (molar ratio of 1:2) and water content (25%, v/v), time 20 min, microwave power 600 W, temperature 62 ℃, liquid/solid ratio 26 mL/g. Read More

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http://dx.doi.org/10.1016/j.jpba.2018.12.032DOI Listing
December 2018
3 Reads
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Stability of meropenem in plasma versus dried blood spots (DBS).

J Pharm Biomed Anal 2019 Mar 25;170:279-284. Epub 2019 Mar 25.

Institute of Clinical Pharmacology, Otto-von-Guericke University, Magdeburg, Germany.

Meropenem, a beta-lactam antibiotic belonging to the group of carbapenems, is widely used in the treatment of serious and complicated infections. Therapeutic drug monitoring (TDM) is strongly recommended to achieve therapeutic success, but the limited stability of the drug in plasma makes transport between clinic and laboratory difficult. The aim of this study was to investigate whether the stability of meropenem was improved in dried blood spots (DBS) and whether sample transport between clinic and laboratory could be simplified by using this medium. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.055DOI Listing
March 2019
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Quantification of estradiol cypionate in plasma by liquid chromatography coupled with tandem mass spectrometry: Application in a pharmacokinetic study in healthy female volunteers.

J Pharm Biomed Anal 2019 Mar 26;170:273-278. Epub 2019 Mar 26.

Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil; Faculty of Medicine Sao Leopoldo Mandic, Campinas, SP, Brazil; Faculty of Medicine, University of Mogi das Cruzes, Mogi das Cruzes, Brazil.

The combination of medroxyprogesterone acetate 25 mg + estradiol cypionate 5 mg is a highly effective, monthly injectable contraceptive. For the first time, this study presents the development and validation of a sensitive method for estradiol cypionate analysis in human plasma by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Aliquots (500 μL) of plasma were extracted with ethyl ether (100%) and derivatized with dansyl chloride. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.053DOI Listing
March 2019
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Pharmacokinetic study on bruceoside A revealed the potential role of quassinoid glycosides for the anticancer properties of Fructus Bruceae.

J Pharm Biomed Anal 2019 Mar 23;170:264-272. Epub 2019 Mar 23.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Bruceoside A, an abundant quassinoid glycoside in Fructus Bruceae, was chosen for the pharmacokinetic study. It is the first case report on the pharmacokinetic study of quassinoid glycosides so far. A sensitive, accurate, and repeatable UHPLC-MS/MS method was developed for the determination of bruceoside A and its major metabolite. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.052DOI Listing
March 2019
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Determination of kansuiphorin C and kansuinin A in rat feces using UFLC-MS/MS and its application in the comparative excretion study on normal and malignant ascites rats.

J Pharm Biomed Anal 2019 Mar 26;170:254-263. Epub 2019 Mar 26.

Jiangsu Key Laboratory for High Technology Research of TCM Formulae, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, No. 138, Xianlin Road, Qixia District, Nanjing, 210023, PR China. Electronic address:

Malignant ascites (MA) is one of the severe complications of gastrointestinal tumors, affecting the patients' survival time and quality of life. Euphorbia kansui is a commonly used toxic Chinese herbal medicine for malignant ascites. Our previous study showed that the biological and toxicological effects of kansui were closely related to the gastrointestinal tract. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.054DOI Listing
March 2019
2 Reads
2.979 Impact Factor

New HPLC-MS method for rapid and simultaneous quantification of doxycycline, diethylcarbamazine and albendazole metabolites in rat plasma and organs after concomitant oral administration.

J Pharm Biomed Anal 2019 Mar 26;170:243-253. Epub 2019 Mar 26.

School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK. Electronic address:

A sensitive and relatively fast, cost-effective high-performance liquid chromatographic method coupled with mass spectrometer (HPLC-MS) is herein reported for the first time for a simultaneous quantification of plasma and organs concentration of three therapeutic agents that are widely used in treatment of lymphatic filariasis (LF), namely, doxycycline (DOX), diethylcarbamazine (DEC) and albendazole (ABZ) metabolites. The method was developed and validated as per ICH and FDA guidelines and successfully employed to quantify DOX, DEC and ABZ metabolites (albendazole sulfoxide (ABZ-OX) and albendazole sulfone (ABZ-ON)) in the plasma and organs of Sprague Dawley rats after oral concomitant administration of the above mentioned therapeutic agents. Importantly, a simple, one-step protein precipitation and extraction method was used to extract the four compounds efficiently with a recovery in the range of 79. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.047DOI Listing
March 2019
4 Reads
2.979 Impact Factor

Characterization of the species-specific acid-base equilibria of adrenaline and noradrenaline.

J Pharm Biomed Anal 2019 Feb 22;170:215-219. Epub 2019 Feb 22.

Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hungary; Research Group of Drugs of Abuse and Doping Agents, Hungarian Academy of Sciences, Hungary. Electronic address:

Adrenaline, noradrenaline, the biogenic catecholamines of vital importance, and four closely related compounds were studied by H NMR-pH titrations, and the resulting acid-base properties are quantified in terms of three macroscopic and twelve microscopic protonation constants for both molecules. The species-specific basicities are interpreted by means of inductive and shielding effects by comparing the protonation constants of the catecholamines, including dopamine. The site-specific basicities determined this way could be key parameters for the interpretation of biochemical behavior. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.035DOI Listing
February 2019
2.979 Impact Factor

Rapid multiresidue analysis of authorized/banned cyclopolypeptide antibiotics in feed by liquid chromatography-tandem mass spectrometry based on dispersive solid-phase extraction.

J Pharm Biomed Anal 2019 Mar 26;170:234-242. Epub 2019 Mar 26.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

An effective analytical strategy was developed for simultaneous determination of seven cyclopolypeptide antibiotics (vancomycin, polymyxin B, polymyxin E, teicoplanin A2, bacitracin A, daptomycin and virginiamycin M1) in feed using liquid chromatography-tandem mass spectrometry. The extraction of sample was based on acidified methanol-aqueous solution, followed by a simply dispersive solid-phase extraction for further purification with primary secondary amine as an adsorbent. The method showed good linearity for target analytes in the experimental concentration ranges. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.050DOI Listing
March 2019
2 Reads

Development and validation of a green method for dissolution monitoring of pharmaceutical combinations. Meloxican and pridinol.

J Pharm Biomed Anal 2019 Mar 18;170:228-233. Epub 2019 Mar 18.

Área de Análisis de Medicamentos, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario e Instituto de Química Rosario (IQUIR, CONICET-UNR), Argentina. Electronic address:

The development of a chemometric method for monitoring the pharmaceutical dissolution, under green analytical chemistry principles, was reported. Meloxicam (MEL) and pridinol (PRI) were employed as a combination model. Multivariate curve resolution with alternating least squares (MCR-ALS) was proposed to resolve UV spectra of the analytes during pharmaceutical dissolution. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.038DOI Listing

Target exposure and pharmacodynamics study of the indoleamine 2,3-dioxygenase-1 (IDO-1) inhibitor epacadostat in the CT26 mouse tumor model.

J Pharm Biomed Anal 2019 Mar 22;170:220-227. Epub 2019 Mar 22.

ImaBiotech SAS, Parc Eurasanté, 152 rue du Dr Yersin, 59120, Loos, France; ImaBiotech Corp, 44 Manning Rd, MA, 01821, Billerica, United States. Electronic address:

Indoleamine-2,3-dioxygénase (IDO1) is an enzyme which converts tryptophan (Trp) into kynurenine (Kyn). Having a critical role in tumor immune escape by decreasing Trp and increasing Kyn levels in the microenvironment, IDO1 was one of the first targets for small molecules drug discovery in the field of immuno-oncology. A potent and selective IDO1 inhibitor such as Epacadostat (EPA) was shown to enhance the antitumor activity by restoring the immune system fitness. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.038DOI Listing

Deciphering the intervention mechanism of Taohong Siwu Decoction following the abnormal uterine bleeding rats based on serum metabolic profiles.

J Pharm Biomed Anal 2019 Mar 23;170:204-214. Epub 2019 Mar 23.

School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China; AnHui Province Key Laboratory of Chinese Medicinal Formula, Hefei, Anhui, 230012, China. Electronic address:

Abnormal uterine bleeding (AUB), one of the most significant characters of incomplete abortion, is a widespread phenomenon in gynecological that put a woman into a terrible physiological and psychological state. Taohong Siwu Decoction (TSD) is a traditional Chinese medicine (TCM) prescriptions which have treated AUB in China for decades. Our previous study elucidated that TSD reduced the volume of uterine bleedings as well as repaired the endometrium. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.051DOI Listing
March 2019
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An optimized TaqMan real-time PCR method for authentication of ASINI CORII COLLA (donkey-hide gelatin).

J Pharm Biomed Anal 2019 Mar 15;170:196-203. Epub 2019 Mar 15.

National Institutes for Food and Drug Control, NO. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, PR China. Electronic address:

In this study, probe/primers of high specificity and sensitivity were selected to analyze donkey-hide gelatin for donkey DNA and to look for horse, ox, and pig DNA as possible adulterants. The mitochondrial CO I genes in donkey, horse, and ox were selected as target sequences for design and synthesis of three pairs of specific probes and primers. In addition, eight pairs of probe/primers were obtained via literature search. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.028DOI Listing
March 2019
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Potential pitfalls in LC-MS/MS quantification of colistin for therapeutic drug monitoring of patients treated with colistimethate.

J Pharm Biomed Anal 2019 Mar 14;170:193-195. Epub 2019 Mar 14.

Central Laboratory of Analyses, IRCCS Istituto Giannina Gaslini, Genoa, Italy. Electronic address:

Starting from a warning of possible artificially high plasma colistin concentrations observed in clinical samples in our recently published LC-MS/MS method, we reviewed our analytical assay and demonstrated, for the first time, that caution must be paid to ionization conditions employed in the MS system. The artefact reported here demonstrates that additional experiments should be performed in the course of validation of LC-MS/MS methods to be used in samples containing both colistin and colistimethate. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.023DOI Listing
March 2019
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Quantitative determination and pharmacokinetic study of fusaricidin A in mice plasma and tissues using ultra-high performance liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2019 Mar 19;170:187-192. Epub 2019 Mar 19.

National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA. Electronic address:

Fusaricidins are a family of cyclic lipodepsipeptides that convey antifungal and antibacterial activity. Fusaricidin A (FA) is one of the Fusaricidins major compounds and it is showing promising activity against fungi and bacteria. In the present study, a fast and sensitive ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for the analysis of FA in mice plasma, liver, kidney and brain tissues. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.042DOI Listing
March 2019
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Pharmacokinetics and metabolism of GW9508 in rat by liquid chromatography/electrospray ionization tandem mass spectrometry.

J Pharm Biomed Anal 2019 Mar 19;170:176-186. Epub 2019 Mar 19.

Department of Pharmacy, Maternal and Child Health Care Hospital of Zaozhuang, No. 25 East Cultural Road, Zaozhuang 277100, China. Electronic address:

In this study, a simple, fast and sensitive LC/MS/MS method was developed and validated for the determination of GW9508 in rat plasma. The sample was precipitated with acetonitrile and subsequently separated on ZORBAX Eclipse XDB C column (50 mm × 2.1 mm, 5 μm). Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.040DOI Listing

Analytical human biomonitoring method for the identification and quantification of the metabolite BDCPP originated from the organophosphate flame retardant TDCPP in urine.

J Pharm Biomed Anal 2019 Mar 19;170:169-175. Epub 2019 Mar 19.

TNO, Department Environmental Modelling, Sensing and Analysis (EMSA), Princetonlaan 8, 3584 CB, Utrecht, The Netherlands.

Tris(1,3-dichloropropyl) phosphate (TDCPP, CAS 13674-87-8) is one of the most commonly used organophosphate flame retardants (OPFRs) in cars, residential furniture and other products containing polyurethane foam to meet the required flammability standards. For the tasks of the working group Analyses in Biological Material from the German Research Foundation (DFG), a human biomonitoring process for TDCPP is developed. The metabolism of TDCPP is described in different in vivo studies and it is already shown that Bis (1,3-dichloropropyl) phosphate (BDCPP, CAS 72236-72-7) is the primary compound specific metabolite of TDCPP which is often detectable in urine samples. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.03.036DOI Listing
March 2019
1 Read