1,836 results match your criteria Journal of muscle research and cell motility[Journal]


Neuregulin-1β attenuates sepsis-induced diaphragm atrophy by activating the PI3K/Akt signaling pathway.

J Muscle Res Cell Motil 2019 Apr 16. Epub 2019 Apr 16.

Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Hai Ning Road, Shanghai, 200080, China.

The aim of this study was to investigate the protective effects of neuregulin-1β (NRG-1β) on sepsis-induced diaphragm atrophy and the possible underlying mechanisms. Sprague-Dawley rats were randomly divided into sham, sepsis and NRG groups. Sepsis was induced by cecal ligation and puncture (CLP). Read More

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http://dx.doi.org/10.1007/s10974-019-09512-2DOI Listing

Rapid time-stamped analysis of filament motility.

J Muscle Res Cell Motil 2019 Apr 10. Epub 2019 Apr 10.

Department of Medicine, McGill University, Montreal, QC, Canada.

The in vitro motility assay is a valuable tool to understand motor protein mechanics, but existing algorithms are not optimized for accurate time resolution. We propose an algorithm that combines trace detection with a time-stamped analysis. By tracking filament ends, we minimize data loss from overlapping and crossing filaments. Read More

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http://dx.doi.org/10.1007/s10974-019-09503-3DOI Listing

The role of satellite and other functional cell types in muscle repair and regeneration.

J Muscle Res Cell Motil 2019 Apr 9. Epub 2019 Apr 9.

College of Animal Sciences, Zhejiang University; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Zhejiang Provincial Laboratory of Feed and Animal Nutrition, No. 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.

Skeletal muscles play essential roles in physiological processes, including motor function, energy hemostasis, and respiration. Skeletal muscles also have the capacity to regenerate after injury. Regeneration of skeletal muscle is an extremely complex biological process, which involves multiple cell types. Read More

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http://dx.doi.org/10.1007/s10974-019-09511-3DOI Listing

Colon 26 adenocarcinoma (C26)-induced cancer cachexia impairs skeletal muscle mitochondrial function and content.

J Muscle Res Cell Motil 2019 Apr 3. Epub 2019 Apr 3.

Department of Physical Therapy, University of Florida, 1275 Center Drive, Gainesville, FL, 32610, USA.

The present study aimed to determine the impact of colon 26 adenocarcinoma (C26)-induced cancer cachexia on skeletal muscle mitochondrial respiration and content. Twelve male CD2F1 mice were injected with C26-cells (tumor bearing (TB) group), whereas 12 age-matched mice received PBS vehicle injection (non-tumor bearing (N-TB) group). Mitochondrial respiration was studied in saponin-permeabilized soleus myofibers. Read More

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http://dx.doi.org/10.1007/s10974-019-09510-4DOI Listing
April 2019
2 Reads

A mechanical model of the half-sarcomere which includes the contribution of titin.

J Muscle Res Cell Motil 2019 Mar 21. Epub 2019 Mar 21.

PhysioLab, University of Florence, Via Sansone, 1 - 50019, Sesto Fiorentino, Italy.

The evidence, in both resting and active muscle, for the presence of an I-band spring element like titin that anchors the Z line to the end of the thick filament did not yet produce a proper theoretical treatment in a complete model of the half-sarcomere. The textbook model developed by A. F. Read More

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http://dx.doi.org/10.1007/s10974-019-09508-yDOI Listing
March 2019
1 Read

Baboon (Papio ursinus) single fibre contractile properties are similar to that of trained humans.

J Muscle Res Cell Motil 2019 Mar 20. Epub 2019 Mar 20.

Division of Exercise Science and Sports Medicine, Department of Human Biology, University of Cape Town, PO Box 115, Newlands, 7725, South Africa.

This study investigated the contractile properties of single skeletal muscle fibres from the Vastus lateralis (VL) of two male adult chachma baboons (Papio ursinus) and compared it to that from five male human cyclists. Species comparisons are observational and statistical analyses were not performed due to the low sample size. The histological analyses revealed that the baboon muscles contained more type II fibres than their human counterparts. Read More

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http://dx.doi.org/10.1007/s10974-019-09509-xDOI Listing
March 2019
1 Read

Proteomic profiling of the mouse diaphragm and refined mass spectrometric analysis of the dystrophic phenotype.

J Muscle Res Cell Motil 2019 Mar 19. Epub 2019 Mar 19.

Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland.

The diaphragm is a crucial muscle involved in active inspiration and whole body homeostasis. Previous biochemical, immunochemical and cell biological investigations have established the distribution and fibre type-specific expression of key diaphragm proteins. Building on these findings, it was of interest to establish the entire experimentally assessable diaphragm proteome and verify the presence of specific protein isoforms within this specialized subtype of skeletal muscle. Read More

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http://dx.doi.org/10.1007/s10974-019-09507-zDOI Listing
March 2019
1 Read

Comparative analysis of widely used methods to remove nonfunctional myosin heads for the in vitro motility assay.

J Muscle Res Cell Motil 2019 Mar 8. Epub 2019 Mar 8.

Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, 391 82, Sweden.

The in vitro motility assay allows studies of muscle contraction through observation of actin filament propulsion by surface-adsorbed myosin motors or motor fragments isolated from muscle. A possible problem is that motility may be compromised by nonfunctional, "dead", motors, obtained in the isolation process. Here we investigate the effects on motile function of two approaches designed to eliminate the effects of these dead motors. Read More

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http://dx.doi.org/10.1007/s10974-019-09505-1DOI Listing
March 2019
2.086 Impact Factor

A brief history of M. C. Schaub's legacies: a life dedicated to heart and muscle research : In memoriam Marcus C. Schaub (1936-2018).

J Muscle Res Cell Motil 2018 Aug;39(3-4):61-63

Department of Anesthesiology and Pain Medicine, Department of Pharmacology, Cardiovascular Research Centre, University of Alberta, CSB Room 2-150, Edmonton, AB, T6G 2G3, Canada.

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http://dx.doi.org/10.1007/s10974-019-09502-4DOI Listing

Increase in phospholamban content in mouse skeletal muscle after denervation.

J Muscle Res Cell Motil 2019 Feb 26. Epub 2019 Feb 26.

Department of Molecular Pharmacology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.

It is well-known that denervation of motor nerves induces atrophy and decreases contractile force of the skeletal muscle. However, it is not completely understood how denervation alters calcium handling in the skeletal muscle. We investigated the effect of denervation on the expression and function of proteins involved in calcium handling. Read More

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http://dx.doi.org/10.1007/s10974-019-09504-2DOI Listing
February 2019

A new twist on tropomyosin binding to actin filaments: perspectives on thin filament function, assembly and biomechanics.

J Muscle Res Cell Motil 2019 Feb 15. Epub 2019 Feb 15.

Department of Biological Sciences, University of Massachusetts-Lowell, Lowell, MA, USA.

Tropomyosin, best known for its role in the steric regulation of muscle contraction, polymerizes head-to-tail to form cables localized along the length of both muscle and non-muscle actin-based thin filaments. In skeletal and cardiac muscles, tropomyosin, under the control of troponin and myosin, moves in a cooperative manner between blocked, closed and open positions on filaments, thereby masking and exposing actin-binding sites necessary for myosin crossbridge head interactions. While the coiled-coil signature of tropomyosin appears to be simple, closer inspection reveals surprising structural complexity required to perform its role in steric regulation. Read More

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http://dx.doi.org/10.1007/s10974-019-09501-5DOI Listing
February 2019

47th European Muscle Conference in Budapest, Hungary.

Authors:

J Muscle Res Cell Motil 2018 Aug;39(3-4):65-115

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http://dx.doi.org/10.1007/s10974-018-9500-5DOI Listing
August 2018
1 Read

Acidosis affects muscle contraction by slowing the rates myosin attaches to and detaches from actin.

J Muscle Res Cell Motil 2018 Aug 31;39(3-4):135-147. Epub 2018 Oct 31.

Department of Mathematics, University of California, Davis, Davis, CA, 95616, USA.

The loss of muscle force and power during fatigue from intense contractile activity is associated with, and likely caused by, elevated levels of phosphate ([Formula: see text]) and hydrogen ions (decreased pH). To understand how these deficits in muscle performance occur at the molecular level, we used direct measurements of mini-ensembles of myosin generating force in the laser trap assay at pH 7.4 and 6. Read More

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http://link.springer.com/10.1007/s10974-018-9499-7
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http://dx.doi.org/10.1007/s10974-018-9499-7DOI Listing
August 2018
6 Reads

Specific effects of neuregulin-1β on the communication between DRG neurons and skeletal muscle cells in vitro.

J Muscle Res Cell Motil 2018 Aug 12;39(3-4):117-134. Epub 2018 Sep 12.

Department of Anatomy, Shandong University School of Basic Medical Sciences, 44 Wenhua Xi Road, Jinan, 250012, Shandong, China.

The communication between primary afferent neuron and skeletal muscle (SKM) is one of the important factors on maintaining the structure and function of SKM cells. Neuregulin-1β (NRG-1β) signaling is essential for regulating synaptic neurotransmission. Here, we established a neuromuscular coculture model of dorsal root ganglion (DRG) sensory neurons and SKM cells to explore the nerve-muscle communication in the presence of exogenous NRG-1β. Read More

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http://link.springer.com/10.1007/s10974-018-9498-8
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http://dx.doi.org/10.1007/s10974-018-9498-8DOI Listing
August 2018
8 Reads

A study of the mechanisms of excitation-contraction coupling in frog skeletal muscle based on measurements of [Ca] transients inside the sarcoplasmic reticulum.

J Muscle Res Cell Motil 2018 Apr 24;39(1-2):41-60. Epub 2018 Aug 24.

Departamento de Biofísica, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, CP 11800, Montevideo, Uruguay.

[Ca] transients inside the sarcoplasmic reticulum (SR) were recorded in frog skeletal muscle twitch fibers under voltage clamp using the low affinity indicator Mag Fluo 4 (loaded in its AM form) with the purpose of studying the effect on Ca release of extrinsic Ca buffers (i.e. BAPTA) added at high concentration to the myoplasm. Read More

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http://link.springer.com/10.1007/s10974-018-9497-9
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http://dx.doi.org/10.1007/s10974-018-9497-9DOI Listing
April 2018
16 Reads

Cold shock protein RBM3 attenuates atrophy and induces hypertrophy in skeletal muscle.

J Muscle Res Cell Motil 2018 Apr 26;39(1-2):35-40. Epub 2018 Jul 26.

Department Rehabilitation Sciences, College of Health Sciences, University of Kentucky, 900 S. Limestone, RM 210E, Lexington, KY, 40536-0200, USA.

RNA-binding motif protein 3 (RBM3), a stress-inducible RNA-binding protein that increases protein synthesis and confers cell protection in multiple cell types, has been identified as a possible regulator of skeletal muscle mass. Therefore, the primary aim of this study was to examine the impact of elevated RBM3 on skeletal muscle hypertrophy and resistance to atrophy. Plasmid-mediated overexpression of RBM3 in vitro and in vivo was used to assess the role of RBM3 in muscle. Read More

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http://dx.doi.org/10.1007/s10974-018-9496-xDOI Listing
April 2018
5 Reads

Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.

J Muscle Res Cell Motil 2018 Apr 15;39(1-2):1-16. Epub 2018 Jun 15.

Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.

Laboratory rats are sedentary if housed in conditions where activity is limited. Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks. EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined. Read More

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http://dx.doi.org/10.1007/s10974-018-9493-0DOI Listing
April 2018
5 Reads

BGP-15 improves contractile function of regenerating soleus muscle.

J Muscle Res Cell Motil 2018 Apr 11;39(1-2):25-34. Epub 2018 Jun 11.

Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av Prof. Lineu Prestes, 2415, São Paulo, SP, 05508-000, Brazil.

This study investigated the effect of the heat shock protein inducer O-[3-piperidino-2-hydroxy-1-propyl]-nicotinic amidoxime (BGP-15) on the morphology and contractile function of regenerating soleus muscles from mice. Cryolesioned soleus muscles from young mice treated daily with BGP-15 (15 mg/Kg) were evaluated on post-cryolesion day 10. At this time point, there was a significant decrease in the cross-sectional area of regenerating myofibers, maximal force, specific tetanic force, and fatigue resistance of regenerating soleus muscles. Read More

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http://dx.doi.org/10.1007/s10974-018-9495-yDOI Listing
April 2018
10 Reads

The expression of aquaporin-4 is regulated based on innervation in skeletal muscles.

J Muscle Res Cell Motil 2018 Apr 4;39(1-2):17-23. Epub 2018 Jun 4.

Section for Health-related Physical Education, Division of Human Sciences, Faculty of Engineering, Osaka Institute of Technology, Ohmiya, Asahi-ku, 535-8585, Osaka, Japan.

Aquaporin-4 (AQP4) is a selective water channel, which expresses on the plasma membrane of myofibers and regulates the osmotic pressure, energy metabolism and morphological changes in myofibers by modulating water transport across sarcolemma in skeletal muscles. Although the physiological roles of AQP4 have been gradually clarified in skeletal muscles, the regulatory mechanisms of AQP4 expression have been poorly understood in skeletal muscles. Recently, it was reported that the expression of AQP4 decreased in atrophied skeletal muscles following sciatic nerve transection, but not tail-suspension. Read More

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http://dx.doi.org/10.1007/s10974-018-9494-zDOI Listing
April 2018
2 Reads

Development of apical hypertrophic cardiomyopathy with age in a transgenic mouse model carrying the cardiac actin E99K mutation.

J Muscle Res Cell Motil 2017 Dec 26;38(5-6):421-435. Epub 2018 Mar 26.

Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, 52242, USA.

In both humans and mice, the Glu-99-Lys (E99K) mutation in the cardiac actin gene (ACTC) results in little understood apical hypertrophic cardiomyopathy (AHCM). To determine how cross-bridge kinetics change with AHCM development, we applied sinusoidal length perturbations to skinned papillary muscle fibres from 2- and 5-month old E99K transgenic (Tg) and non-transgenic (NTg) mice, and studied tension and its transients. These age groups were chosen because our preliminary studies indicated that AHCM develops with age. Read More

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http://dx.doi.org/10.1007/s10974-018-9492-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003854PMC
December 2017
17 Reads
2.090 Impact Factor

Hans-Christoph Lüttgau 20 July 1926-5 November 2017.

J Muscle Res Cell Motil 2017 Dec;38(5-6):399-403

School of Life Sciences, University of Glasgow, University Avenue, Glasgow, G12 8QQ, UK.

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http://dx.doi.org/10.1007/s10974-018-9491-2DOI Listing
December 2017
4 Reads

Abstracts of EMC2017.

Authors:

J Muscle Res Cell Motil 2017 Dec 7:339-398. Epub 2017 Dec 7.

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http://dx.doi.org/10.1007/s10974-017-9490-8DOI Listing
December 2017
18 Reads

Are mechanically sensitive regulators involved in the function and (patho)physiology of cerebral palsy-related contractures?

J Muscle Res Cell Motil 2017 Aug 30;38(3-4):317-330. Epub 2017 Nov 30.

Exercise Physiology Research Group, Department of Movement Sciences, Biomedical Sciences Group, KU Leuven, Tervuursevest 101, box 1500, 3001, Leuven, Belgium.

Skeletal muscle tissue is mechanosensitive, as it is able to sense mechanical impacts and to translate these into biochemical signals making the tissue adapt. Among its mechanosensitive nature, skeletal muscle tissue is the largest metabolic organ of the human body. Disturbances in skeletal muscle mechanosensing and metabolism cause and contribute to many diseases, i. Read More

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http://dx.doi.org/10.1007/s10974-017-9489-1DOI Listing
August 2017
4 Reads

Changes in contractile and metabolic parameters of skeletal muscle as rats age from 3 to 12 months.

J Muscle Res Cell Motil 2017 Dec 28;38(5-6):405-420. Epub 2017 Nov 28.

Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.

Laboratory rats are considered mature at 3 months despite that musculoskeletal growth is still occurring. Changes in muscle physiological and biochemical characteristics during development from 3 months, however, are not well understood. Whole muscles and single skinned fibres from fast-twitch extensor digitorum longus (EDL) and predominantly slow-twitch soleus (SOL) muscles were examined from male Sprague-Dawley rats (3, 6, 9, 12 months). Read More

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http://dx.doi.org/10.1007/s10974-017-9484-6DOI Listing
December 2017
7 Reads

Editorial on EMC 2017 special issue.

Authors:
Martina Krüger

J Muscle Res Cell Motil 2017 Aug 23;38(3-4):271-273. Epub 2017 Nov 23.

Institute of Cardiovascular Physiology, Düsseldorf, Germany.

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http://dx.doi.org/10.1007/s10974-017-9485-5DOI Listing
August 2017
9 Reads

Obituary Bernhard Brenner.

J Muscle Res Cell Motil 2017 Aug;38(3-4):269-270

Medizinische Hochschule Hannover, Hannover, Germany.

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http://dx.doi.org/10.1007/s10974-017-9488-2DOI Listing
August 2017
7 Reads

When signalling goes wrong: pathogenic variants in structural and signalling proteins causing cardiomyopathies.

J Muscle Res Cell Motil 2017 Aug 8;38(3-4):303-316. Epub 2017 Nov 8.

Division of Cardiovascular Medicine, Radcliffe Department of Medicine and British Heart Foundation Centre of Research Excellence, University of Oxford, Oxford, UK.

Cardiomyopathies are a diverse group of cardiac disorders with distinct phenotypes, depending on the proteins and pathways affected. A substantial proportion of cardiomyopathies are inherited and those will be the focus of this review article. With the wide application of high-throughput sequencing in the practice of clinical genetics, the roles of novel genes in cardiomyopathies are recognised. Read More

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http://link.springer.com/10.1007/s10974-017-9487-3
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http://dx.doi.org/10.1007/s10974-017-9487-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742121PMC
August 2017
10 Reads

Intrinsic MYH7 expression regulation contributes to tissue level allelic imbalance in hypertrophic cardiomyopathy.

J Muscle Res Cell Motil 2017 Aug 3;38(3-4):291-302. Epub 2017 Nov 3.

Institute of Molecular and Cell Physiology, Hannover Medical School, Hanover, Germany.

HCM, the most common inherited cardiac disease, is mainly caused by mutations in sarcomeric genes. More than a third of the patients are heterozygous for mutations in the MYH7 gene encoding for the β-myosin heavy chain. In HCM-patients, expression of the mutant and the wildtype allele can be unequal, thus leading to fractions of mutant and wildtype mRNA and protein which deviate from 1:1. Read More

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http://dx.doi.org/10.1007/s10974-017-9486-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742120PMC
August 2017
28 Reads

Anisotropic mechanosensitive pathways in the diaphragm and their implications in muscular dystrophies.

J Muscle Res Cell Motil 2017 Dec 6;38(5-6):437-446. Epub 2017 Oct 6.

Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.

The diaphragm is the "respiratory pump;" the muscle that generates pressure to allow ventilation. Diaphragm muscles play a vital function and thus are subjected to continuous mechanical loading. One of its peculiarities is the ability to generate distinct mechanical and biochemical responses depending on the direction through which the mechanical forces applied to it. Read More

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http://dx.doi.org/10.1007/s10974-017-9483-7DOI Listing
December 2017
8 Reads

Kinetic coupling of phosphate release, force generation and rate-limiting steps in the cross-bridge cycle.

J Muscle Res Cell Motil 2017 Aug 16;38(3-4):275-289. Epub 2017 Sep 16.

Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni, 63, 50134, Firenze, Italy.

A basic goal in muscle research is to understand how the cyclic ATPase activity of cross-bridges is converted into mechanical force. A direct approach to study the chemo-mechanical coupling between P release and the force-generating step is provided by the kinetics of force response induced by a rapid change in [P]. Classical studies on fibres using caged-P discovered that rapid increases in [P] induce fast force decays dependent on final [P] whose kinetics were interpreted to probe a fast force-generating step prior to P release. Read More

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http://dx.doi.org/10.1007/s10974-017-9482-8DOI Listing
August 2017
9 Reads

Spot light on skeletal muscles: optogenetic stimulation to understand and restore skeletal muscle function.

J Muscle Res Cell Motil 2017 Aug 16;38(3-4):331-337. Epub 2017 Sep 16.

Institute of Physiology I, Medical Faculty, University of Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany.

Damage of peripheral nerves results in paralysis of skeletal muscle. Currently, the only treatment option to restore proper function is electrical stimulation of the innervating nerve or of the skeletal muscles directly. However this approach has low spatial and temporal precision leading to co-activation of antagonistic muscles and lacks cell-type selectivity resulting in pain or discomfort by stimulation of sensible nerves. Read More

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http://dx.doi.org/10.1007/s10974-017-9481-9DOI Listing
August 2017
8 Reads

Are there two different binding sites for ATP on the myosin head, or only one that switches between two conformers?

J Muscle Res Cell Motil 2017 04;38(2):137-142

Centre de Biochimie Structurale (CBS), INSERM, CNRS, Université de Montpellier, Montpellier, France.

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http://dx.doi.org/10.1007/s10974-017-9480-xDOI Listing
April 2017
7 Reads

Insight into muscle physiology through understanding mechanisms of muscle pathology.

J Muscle Res Cell Motil 2017 02 29;38(1):1-2. Epub 2017 Aug 29.

INSERM U1046, CNRS UMR9214, University of Montpellier, Montpellier, France.

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http://dx.doi.org/10.1007/s10974-017-9479-3DOI Listing
February 2017
5 Reads

Proteomic profiling of the dystrophin complex and membrane fraction from dystrophic mdx muscle reveals decreases in the cytolinker desmoglein and increases in the extracellular matrix stabilizers biglycan and fibronectin.

J Muscle Res Cell Motil 2017 04 12;38(2):251-268. Epub 2017 Aug 12.

Department of Biology, Maynooth University, National University of Ireland, Maynooth, County Kildare, Ireland.

The almost complete loss of the membrane cytoskeletal protein dystrophin and concomitant drastic reduction in dystrophin-associated glycoproteins are the underlying mechanisms of the highly progressive neuromuscular disorder Duchenne muscular dystrophy. In order to identify new potential binding partners of dystrophin or proteins in close proximity to the sarcolemmal dystrophin complex, proteomic profiling of the isolated dystrophin-glycoprotein complex was carried out. Subcellular membrane fractionation and detergent solubilisation, in combination with ion exchange, lectin chromatography and density gradient ultracentrifugation, was performed to isolate a dystrophin complex-enriched fraction. Read More

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http://dx.doi.org/10.1007/s10974-017-9478-4DOI Listing
April 2017
20 Reads

Structure of isolated Z-disks from honeybee flight muscle.

J Muscle Res Cell Motil 2017 04 21;38(2):241-250. Epub 2017 Jul 21.

Astbury Center, University of Leeds, Leeds, LS2 9JT, UK.

The Z-disk is a complex structure comprising some 40 proteins that are involved in the transmission of force developed during muscle contraction and in important signalling pathways that govern muscle homeostasis. In the Z-disk the ends of antiparallel thin filaments from adjacent sarcomeres are crosslinked by α-actinin. The structure of the Z-disk lattice varies greatly throughout the animal kingdom. Read More

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http://dx.doi.org/10.1007/s10974-017-9477-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660141PMC
April 2017
6 Reads

Myosin II sequences for Lethocerus indicus.

J Muscle Res Cell Motil 2017 04 13;38(2):193-200. Epub 2017 Jul 13.

Department of Cell Biology, Duke University, Box 3011, Durham, NC, 27705, USA.

We present the genomic and expressed myosin II sequences from the giant waterbug, Lethocerus indicus. The intron rich gene appears relatively ancient and contains six regions of mutually exclusive exons that are alternatively spliced. Alternatively spliced regions may be involved in the asymmetric myosin dimer structure known as the interacting heads motif, as well as stabilizing the interacting heads motif within the thick filament. Read More

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http://dx.doi.org/10.1007/s10974-017-9476-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660136PMC
April 2017
11 Reads

The Secretory Leukocyte Protease Inhibitor mRNA expression is involved with inflammatory control after downhill exercise in the triceps brachii intermediary head in Wistar rats.

J Muscle Res Cell Motil 2017 04 30;38(2):231-239. Epub 2017 Jun 30.

Departamento de Biociências - Campus da Baixada Santista, Universidade Federal de São Paulo, UNIFESP, Rua Silva Jardim, 136, Vila Mathias, Santos, SP, CEP: 11015-020, Brazil.

After severe skeletal muscle damage, communication between inflammatory macrophages, myogenic cells, and modulatory secretion factors is essential to induce re-establishment of skeletal muscle structure. To analyze when characteristic gene expression of macrophages, myogenic cells, and SLPI are modulated after an exercise-induced muscle damage (EIMD) downhill protocol. Twenty-six rats were exposed to an experimental protocol of exercise and euthanized before (CTRL), immediately after (G0), and 24 (G24) and 48 (G48) hours after the exercise. Read More

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http://dx.doi.org/10.1007/s10974-017-9475-7DOI Listing
April 2017
16 Reads

Physiology and pathophysiology of excitation-contraction coupling: the functional role of ryanodine receptor.

J Muscle Res Cell Motil 2017 02 26;38(1):37-45. Epub 2017 Jun 26.

The Wu Center for Molecular Cardiology, Columbia University, New York, NY, USA.

Calcium (Ca) release from intracellular stores plays a key role in the regulation of skeletal muscle contraction. The type 1 ryanodine receptors (RyR1) is the major Ca release channel on the sarcoplasmic reticulum (SR) of myocytes in skeletal muscle and is required for excitation-contraction (E-C) coupling. This article explores the role of RyR1 in skeletal muscle physiology and pathophysiology. Read More

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http://dx.doi.org/10.1007/s10974-017-9470-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813681PMC
February 2017
15 Reads

Follistatin treatment suppresses SERCA1b levels independently of other players of calcium homeostasis in C2C12 myotubes.

J Muscle Res Cell Motil 2017 04 21;38(2):215-229. Epub 2017 Jun 21.

Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Follistatin (FS) is a high affinity activin-binding protein, neutralizing the effects of the Transforming Growth Factor-beta (TGF-β) superfamily members, as myostatin (MSTN). Since MSTN emerged as a negative regulator, FS has been considered as a stimulator of skeletal muscle growth and differentiation. Here, we studied the effect of FS administration on the Ca-homeostasis of differentiating C2C12 skeletal muscle cells. Read More

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http://dx.doi.org/10.1007/s10974-017-9474-8DOI Listing
April 2017
16 Reads

Acute high-caffeine exposure increases autophagic flux and reduces protein synthesis in C2C12 skeletal myotubes.

J Muscle Res Cell Motil 2017 04 20;38(2):201-214. Epub 2017 Jun 20.

Division of Natural Sciences and Engineering, University of South Carolina Upstate, Spartanburg, SC, 29303, USA.

Caffeine is a highly catabolic dietary stimulant. High caffeine concentrations (1-10 mM) have previously been shown to inhibit protein synthesis and increase protein degradation in various mammalian cell lines. The purpose of this study was to examine the effect of short-term caffeine exposure on cell signaling pathways that regulate protein metabolism in mammalian skeletal muscle cells. Read More

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http://dx.doi.org/10.1007/s10974-017-9473-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660649PMC
April 2017
6 Reads

Cooperativity of myosin interaction with thin filaments is enhanced by stabilizing substitutions in tropomyosin.

J Muscle Res Cell Motil 2017 04 24;38(2):183-191. Epub 2017 May 24.

Institute of Mechanics, Moscow State University, 1 Mitchurinsky prosp., Moscow, 119192, Russia.

Muscle contraction is powered by myosin interaction with actin-based thin filaments containing Ca-regulatory proteins, tropomyosin and troponin. Coiled-coil tropomyosin molecules form a long helical strand that winds around actin filament and either shields actin from myosin binding or opens it. Non-canonical residues G126 and D137 in the central part of tropomyosin destabilize its coiled-coil structure. Read More

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http://dx.doi.org/10.1007/s10974-017-9472-xDOI Listing
April 2017
14 Reads

Marked decrease of aquaporin-4 protein is independent of the changes in α1-syntrophin and TRPV4 levels in response to denervation-induced muscle atrophy in vivo.

J Muscle Res Cell Motil 2017 04 9;38(2):175-181. Epub 2017 May 9.

Section for Health-related Physical Education, Division of Human Sciences, Faculty of Engineering, Osaka Institute of Technology, Ohmiya, Asahi-ku, Osaka, 535-8585, Japan.

Aquaporin-4 (AQP4) is a selective water channel mediating water transport across cell membranes in skeletal muscles. Recently, it was noted that AQP4 is one of the key molecules regulating muscle morphology. Indeed, the AQP4 accumulation level was stably maintained in hypertrophied skeletal muscles. Read More

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http://dx.doi.org/10.1007/s10974-017-9471-yDOI Listing
April 2017
15 Reads

Epigenetic regulation of muscle development.

J Muscle Res Cell Motil 2017 02 28;38(1):31-35. Epub 2017 Mar 28.

CNRS URA 3738, Department of Developmental and Stem Cell Biology, Institut Pasteur, Stem Cells and Development, 25 rue du Dr. Roux, 75724, Paris Cedex 15, France.

In eukaryote cells, chromatin appears in several forms and is composed of genomic DNA, protein and RNA. The protein content of chromatin is composed primarily of core histones that are packaged into nucleosomes resulting in the condensation of the DNA. Several epigenetic mechanisms regulate the stability of the nucleosomes and the protein-protein interactions that modify the transcriptional activity of the DNA. Read More

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http://dx.doi.org/10.1007/s10974-017-9469-5DOI Listing
February 2017
10 Reads

Energy demand and supply in human skeletal muscle.

Authors:
C J Barclay

J Muscle Res Cell Motil 2017 04 12;38(2):143-155. Epub 2017 Mar 12.

School of Allied Health Sciences, Griffith University, Gold Coast, QLD, 4222, Australia.

The energy required for muscle contraction is provided by the breakdown of ATP but the amount of ATP in muscles cells is sufficient to power only a short duration of contraction. Buffering of ATP by phosphocreatine, a reaction catalysed by creatine kinase, extends the duration of activity possible but sustained activity depends on continual regeneration of PCr. This is achieved using ATP generated by oxidative processes and, during intense activity, by anaerobic glycolysis. Read More

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http://dx.doi.org/10.1007/s10974-017-9467-7DOI Listing
April 2017
5 Reads

Mouse soleus (slow) muscle shows greater intramyocellular lipid droplet accumulation than EDL (fast) muscle: fiber type-specific analysis.

J Muscle Res Cell Motil 2017 04 9;38(2):163-173. Epub 2017 Mar 9.

Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.

Skeletal muscle is the main tissue of lipid metabolism and accordingly is critical for homeostasis and energy production; however, the determinants of lipid accumulation in skeletal muscle are unknown. Here, we examined whether the soleus muscle (predominantly slow-twitch fibers) has a higher lipid accumulation capacity than that of the extensor digitorum longus (EDL, predominantly fast-twitch fibers) muscle in mice. Soleus and EDL muscles were harvested from male C57BL/6J mice. Read More

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http://dx.doi.org/10.1007/s10974-017-9468-6DOI Listing
April 2017
11 Reads

Respiratory muscle contractile inactivity induced by mechanical ventilation in piglets leads to leaky ryanodine receptors and diaphragm weakness.

J Muscle Res Cell Motil 2017 02 4;38(1):17-24. Epub 2017 Mar 4.

Inserm U1046, CNRS UMR 91214, Université de Montpellier, Centre Hospitalier Regional Universitaire de Montpellier, 34295, Montpellier, France.

Respiratory muscle contractile inactivity during mechanical ventilation (MV) induces diaphragm muscle weakness, a condition referred to as ventilator-induced diaphragmatic dysfunction (VIDD). Although VIDD pathophysiological mechanisms are still not fully understood, it has been recently suggested that remodeling of the sarcoplasmic reticulum (SR) calcium release channel/ryanodine receptors (RyR1) in the diaphragm is a proximal mechanism of VIDD. Here, we used piglets, a large animal model of VIDD that is more relevant to human pathophysiology, to determine whether RyR1 alterations are observed in the presence of diaphragm weakness. Read More

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http://dx.doi.org/10.1007/s10974-017-9464-xDOI Listing
February 2017
8 Reads

Shortening speed dependent force potentiation is attenuated but not eliminated in skeletal muscles without myosin phosphorylation.

J Muscle Res Cell Motil 2017 04 2;38(2):157-162. Epub 2017 Mar 2.

Centre for Bone and Muscle Health, Brock University, 274 Walker Complex, 1812 Sir Isaac Brock Way, St. Catharines, ON, L2S 3A1, Canada.

We investigated the influence of shortening speed on concentric force potentiation at different frequencies in muscles devoid of skeletal myosin light chain kinase (skMLCK) and unable to phosphorylate myosin. EDL muscles from skMLCK mice were activated in vitro (25 °C) across a range of stimulation frequencies (10-100 Hz) during shortening ramps at 0.10, 0. Read More

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http://dx.doi.org/10.1007/s10974-017-9465-9DOI Listing
April 2017
7 Reads

The TRPM4 channel is functionally important for the beneficial cardiac remodeling induced by endurance training.

J Muscle Res Cell Motil 2017 02 21;38(1):3-16. Epub 2017 Feb 21.

Physiologie & Médecine Expérimentale du Cœur et des Muscles, INSERM U1046, CNRS UMR 9214, University of Montpellier, 34295, Montpellier cedex 5, France.

Cardiac hypertrophy (CH) is an adaptive process that exists in two distinct forms and allows the heart to adequately respond to an organism's needs. The first form of CH is physiological, adaptive and reversible. The second is pathological, irreversible and associated with fibrosis and cardiomyocyte death. Read More

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http://dx.doi.org/10.1007/s10974-017-9466-8DOI Listing
February 2017
32 Reads

45th European Muscle Conference in Montpellier, France.

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J Muscle Res Cell Motil 2017 02;38(1):47-136

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http://dx.doi.org/10.1007/s10974-016-9457-1DOI Listing
February 2017
11 Reads

Characterization of fast-twitch and slow-twitch skeletal muscles of calsequestrin 2 (CASQ2)-knock out mice: unexpected adaptive changes of fast-twitch muscles only.

J Muscle Res Cell Motil 2016 12 27;37(6):225-233. Epub 2017 Jan 27.

Dipartimento di Scienze Biomediche dell'Università di Padova, Istituto Interuniversitario di Miologia, Viale G. Colombo 3, 35121, Padova, Italy.

This study investigates the functional role of calsequestrin 2 (CASQ2) in both fast-twitch and slow-twitch skeletal muscles by using CASQ2-/- mice; CASQ2 is expressed throughout life in slow-twitch muscles, but only in the developmental and neonatal stages in fast-twitch muscles. CASQ2-/- causes increase in calsequestrin 1 (CASQ1) expression, but without functional changes in both muscle types. CASQ2-/- mice have ultrastructural changes in fast-twitch muscles only, i. Read More

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http://dx.doi.org/10.1007/s10974-016-9463-3DOI Listing
December 2016
8 Reads