16,187 results match your criteria Journal of clinical pathology[Journal]


Detection of mutations using target capture sequencing in plasma of patients with non-small-cell lung cancer.

J Clin Pathol 2019 Feb 20. Epub 2019 Feb 20.

Department of Respiratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China

PURPOSE : Circulating tumour DNA (ctDNA) is a promising biomarker for detection of non-invasive epidermal growth factor receptor ) mutations in patients with non-small-cell lung cancer (NSCLC). However, the existing methods have limitations in sensitivity or in availability. The aim was to evaluate the accuracy of capture target sequencing for detecting mutations in ctDNA. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205699DOI Listing
February 2019

Agar pre-embedding of small skin biopsies: real-life benefits and challenges in high throughput pathology laboratories.

J Clin Pathol 2019 Feb 20. Epub 2019 Feb 20.

Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), Univeristy of Genoa, Genoa, Italy

Paraffin embedding of small, thin tissue samples requires specific expertise for optimal orientation before tissue sectioning. This study evaluates the real-life utility of the agar pre-embedding technique for small skin biopsies with regards to lengthening of work times, problems in orientation (re-embedding) and ancillary techniques (immunohistochemistry and in situ hybridisation) between two high work flow pathology laboratories, one of which routinely uses the agar pre-embedding technique and one which does not. The mean time required for pre-embedding in agar was 30. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205680DOI Listing
February 2019

The adaptation of AABACUS for quality improvement in laboratory workflow analysis ('L-AABACUS').

J Clin Pathol 2019 Mar;72(3):237-243

Laboratory Medicine Program, University Health Network, Toronto, Canada

The ability to effectively monitor key indicators is important for continuous quality improvement in laboratory immunohistochemistry. This article deals specifically with laboratory turnaround time (TAT) as a key delivery indicator and the impact of laboratory workflow on laboratory TATs. While our laboratory has traditionally relied on the manual calculation of slide-TAT (S-TAT) to monitor delivery, we have determined that automated calculation of case-TAT (C-TAT) would be superior as a delivery indicator. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205725DOI Listing

Digital immunohistochemistry implementation, training and validation: experience and technical notes from a large clinical laboratory.

J Clin Pathol 2019 Feb 14. Epub 2019 Feb 14.

Histopathology Department, Bexley Wing, St James University Hospital, Leeds, UK.

Aims: To consider the value proposition of digitisation of clinical immunohistochemistry services, and to develop an approach to digital immunohistochemistry implementation and validation in a large clinical laboratory.

Methods: A methodology for slide scanning in the laboratory was developed, in addition to a novel validation exercise, to allow pathologists to identify the strengths and weaknesses of digital immunohistochemistry reporting, and train in digital immunohistochemistry slide assessment.

Results: A total of 1480 digital immunohistochemistry slides were assessed by 24 consultant pathologists, with complete clinical concordance between the digital and the glass slide assessment observed. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205628DOI Listing
February 2019
1 Read

Detection of free intraperitoneal tumour cells in peritoneal lavage fluid from patients with peritoneal metastasis before and after treatment with pressurised intraperitoneal aerosol chemotherapy (PIPAC).

J Clin Pathol 2019 Feb 12. Epub 2019 Feb 12.

Odense PIPAC Center, Odense University Hospital, Odense, Denmark.

Aims: In this study, we investigated whether free intraperitoneal tumour cells (FITC) were detectable in ascites or peritoneal lavage fluid (PLF) from patients with peritoneal metastasis (PM) before and after treatment with pressurised intraperitoneal aerosol chemotherapy (PIPAC).

Methods: Ascites or PLF retrieved at the first and third PIPAC procedures was analysed by conventional cytology, carcinoembryonic antigen (CEA) and total protein concentration, and quantitative reverse transcriptase PCR (qRT-PCR) for mRNA expression of CEA, epithelial cell adhesion molecule (EpCAM) and cancer antigen 125 (CA-125). Conventional cytology and qRT-PCR were also performed in a negative control group (benign PLF specimens and inflammatory ascites). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205683DOI Listing
February 2019
1 Read

Nuclear factor erythroid 2-related factors 1 and 2 are able to define the worst prognosis group among high-risk diffuse large B cell lymphomas treated with R-CHOEP.

J Clin Pathol 2019 Feb 12. Epub 2019 Feb 12.

Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.

Aims: Oxidative stress markers and antioxidant enzymes have previously been shown to have prognostic value and associate with adverse outcome in patients with diffuse large B cell lymphoma (DLBCL). Nuclear factor erythroid 2-related factor 1 (Nrf1) and factor 2 (Nrf2) are among the principal inducers of antioxidant enzyme production. Kelch ECH associating protein 1 (Keap1) is a negative regulator of Nrf2, and BTB (BR-C, ttk and bab) domain and CNC homolog 1 (Bach1) represses the function of both factors. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205584DOI Listing
February 2019
1 Read

Accuracy of renal tumour biopsy for the diagnosis and subtyping of papillary renal cell carcinoma: analysis of paired biopsy and nephrectomy specimens with focus on discordant cases.

J Clin Pathol 2019 Feb 12. Epub 2019 Feb 12.

Department of Pathology, University Health Network, Toronto, Ontario, Canada.

Aims: Renal tumour biopsy (RTB) is increasingly recognised as a useful diagnostic tool in the management of small renal masses, particularly those that are incidentally found. Intratumoural heterogeneity with respect to morphology, grade and molecular features represents a frequently identified limitation to the use of RTB. While previous studies have evaluated pathological correlation between RTB and nephrectomy, no studies to date have focused specifically on the role of RTB for the diagnosis of papillary renal cell carcinoma (PRCC) and its further subclassification into clinically relevant subtypes. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205655
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February 2019
4 Reads

Benign spindle cell lesions of the breast: a diagnostic approach to solitary fibrous tumour, nodular pseudoangiomatous stromal hyperplasia and nodular fasciitis.

J Clin Pathol 2019 Feb 9. Epub 2019 Feb 9.

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada

Benign spindle cell lesions of the breast include neoplastic and reactive entities that are diagnostically challenging given their rarity and similar histomorphology. Accurate diagnosis on percutaneous core biopsy within this category is essential as some lesions require excision and surveillance, whereas others may be observed. We present three cases of rare benign spindle cell lesions of the breast that reflect the diversity of this group: solitary fibrous tumour, nodular pseudoangiomatous stromal hyperplasia and nodular fasciitis. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205561DOI Listing
February 2019
1 Read

Histopathological evaluation of scleritis.

J Clin Pathol 2019 Feb 5. Epub 2019 Feb 5.

Department of Ophthalmology, USF Health Morsani College of Medicine, Tampa, Florida, USA

The sclera is an uncommon site of primary inflammation. Biopsy is infrequently employed in the evaluation of scleritis, but familiarity with its differential diagnosis is instrumental in ensuring efficient histological evaluation. This review provides a clinical overview of scleritis and describes the context in which scleral biopsy might arise. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205360DOI Listing
February 2019

Additional loss of MSH2 and MSH6 expression in sporadic deficient mismatch repair colorectal cancer due to MLH1 promoter hypermethylation.

J Clin Pathol 2019 Feb 5. Epub 2019 Feb 5.

Pathology, University of Leeds, Leeds, UK

Colorectal cancer (CRC) is common with 3% of cases associated with germline mutations in the mismatch repair pathway characteristic of Lynch syndrome (LS). The UK National Institute for Health and Care Excellence recommends screening for LS in all patients newly diagnosed with CRC, irrespective of age. The Yorkshire Cancer Research Bowel Cancer Improvement Programme includes a regional LS screening service for all new diagnoses of CRC. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205687
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http://dx.doi.org/10.1136/jclinpath-2018-205687DOI Listing
February 2019
2 Reads

Relevance of TP53 for CLL diagnostics.

J Clin Pathol 2019 Feb 2. Epub 2019 Feb 2.

Department of Haematology, Beaumont Hospital, Dublin, Ireland.

disruption in chronic lymphocytic leukaemia (CLL) is a well-established prognostic marker and informs on the appropriate course of treatment for patients. status is commonly assessed by fluorescence in situ hybridisation for del(17 p) and Sanger sequencing for mutations. At present, current screening methods for mutations fail to detect diagnostically relevant mutations potentially leading to inappropriate treatment decisions. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205622DOI Listing
February 2019

gene: its function and dysregulation in cancer.

J Clin Pathol 2019 Jan 29. Epub 2019 Jan 29.

Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Sydney, Australia.

In this review, we summarise the function and structure of telomerase reverse transcriptase () in humans, including its regulation. The dysregulation of telomerase through promoter mutations across a range of cancers is discussed. The molecular mechanism activated by promoter mutations is outlined. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205653
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http://dx.doi.org/10.1136/jclinpath-2018-205653DOI Listing
January 2019
4 Reads

International Endocervical Adenocarcinoma Criteria and Classification (IECC): correlation with adverse clinicopathological features and patient outcome.

J Clin Pathol 2019 Jan 24. Epub 2019 Jan 24.

Department of Laboratory Medicine and Molecular Diagnostics, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada

Aims: The International Endocervical Adenocarcinoma Criteria and Classification (IECC) was recently proposed as an improved method for categorising endocervical adenocarcinoma (EA) into human papillomavirus (HPV)-associated adenocarcinomas (HPVAs) and non-HPV-associated adenocarcinomas (NHPVAs). Such categorisation correlates with patient age and tumour size; however, its association with patient outcome remains to be established.

Methods: Institutional cases of EA with histological material available were selected. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205632DOI Listing
January 2019
2 Reads

Neoadjuvant chemoradiation in locally advanced rectal cancer: the surgeon's perspective.

J Clin Pathol 2019 Feb;72(2):133-134

Division of General Surgery, University of Toronto, Toronto, Ontario, Canada

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http://dx.doi.org/10.1136/jclinpath-2018-205595DOI Listing
February 2019

Breast specimen handling and reporting in the post-neoadjuvant setting: challenges and advances.

J Clin Pathol 2019 Feb;72(2):120-132

Laboratory Medicine Program, University Health Network, Toronto, Ontario, Canada

Neoadjuvant systemic therapy is becoming more commonly used in patients with earlier stages of breast cancer. To assess tumour response to neoadjuvant chemotherapy, pathological evaluation is the gold standard. Depending on the treatment response, the pathological examination of these specimens can be quite challenging. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205598DOI Listing
February 2019
7 Reads

Gross and microscopic changes of liver neoplasms and background hepatic structures following neoadjuvant therapy.

J Clin Pathol 2019 Feb;72(2):112-119

Department of Pathobiology and Laboratory Medicine, The University of Toronto, Toronto, Ontario, Canada

Liver transplantation is a surgical option with curative intent used in the management of some cases of hepatocellular carcinoma and cholangiocarcinoma (hilar, rarely intrahepatic). A number of different therapeutic modalities including ablative techniques, arterially directed therapies, radiation and chemotherapy are used in the neoadjuvant setting prior to liver transplantation with the goals of preventing tumour progression, decreasing post-transplant recurrence and possibly downstaging patients with tumour burden beyond what is acceptable by current transplant criteria. Pathologists evaluating hepatic explants must be aware of these neoadjuvant therapies and the alterations induced by them in both tumourous and non-tumourous tissue. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205596DOI Listing
February 2019
1 Read

Neoadjuvant therapy in gynaecological malignancies: What pathologists need to know.

J Clin Pathol 2019 Feb;72(2):102-111

Department of Anatomical Pathology, Laboratory Medicine Program, University Health Network, Toronto, Ontario, Canada.

In recent times, there has been a growing tendency to treat advanced gynaecological malignancies with neoadjuvant chemotherapy (NACT), with the goal of reducing tumour volume and enhancing operability resulting in optimal cytoreduction. This approach is used in particular for patients with advanced high-grade serous carcinoma of the ovary, fallopian tube or peritoneum. Pathology plays a crucial role in the management of these patients, both before and after NACT. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205634DOI Listing
February 2019

Postmortem microbiology sampling following death in hospital: an ESGFOR task force consensus statement.

J Clin Pathol 2019 Jan 19. Epub 2019 Jan 19.

Microbiology Laboratory, Biology Department, Instituto Nacional de Toxicología y Ciencias Forenses, Las Rozas, Spain.

Postmortem microbiology (PMM) is a valuable tool in the identification of the cause of death and of factors contributory to death where death has been caused by infection. The value of PMM is dependent on careful autopsy planning, appropriate sampling, minimisation of postmortem bacterial translocation and avoidance of sample contamination. Interpretation of PMM results requires careful consideration in light of the clinical history, macroscopic findings and the histological appearances of the tissues. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205365DOI Listing
January 2019

Neurotrophic tropomyosin or tyrosine receptor kinase (NTRK) genes.

Authors:
Runjan Chetty

J Clin Pathol 2019 Mar 13;72(3):187-190. Epub 2019 Jan 13.

Department of Anatomical Pathology, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada

The neurotrophic tropomyosin or tyrosine receptor kinase (NTRK) genes (1-3) are proto-oncogenes that when activated are encountered in a wide array of tumours. The recent advent of very specific and selective inhibitors of their gene fusions makes the NRTK gene fusions actionable. NRTK gene fusions are very characteristic of specific tumours: salivary mammary analogue secretory carcinoma, breast secretory carcinoma, infantile fibrosarcoma and congenital mesoblastic nephroma. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205672DOI Listing

Update on eighth edition American Joint Committee on Cancer classification for Merkel cell carcinoma and histopathological parameters that determine prognosis.

J Clin Pathol 2019 Jan 13. Epub 2019 Jan 13.

Department of Pathology, Dermatopathology Section, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA

Merkel cell carcinoma (MCC) is a rare primary cutaneous neuroendocrine carcinoma. The annual incidence of MCC is increasing in the USA. Timely diagnosis and proper staging of this tumour are crucial as MCC has high rates of regional recurrence and lymph node and distant metastasis. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205504
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http://dx.doi.org/10.1136/jclinpath-2018-205504DOI Listing
January 2019
4 Reads

Microvessel density in breast cancer: the impact of field area on prognostic informativeness.

J Clin Pathol 2019 Jan 10. Epub 2019 Jan 10.

Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.

Aims: Tumour microvessel density (MVD) is assessed by counting vessels in the most vascularised tumour region, the vascular hot spot. Current uncertainty regarding the prognostic role of MVD in breast cancer could, in part, be explained by variations in field area size for MVD assessment. We aimed to identify the field area size that provides the most accurate prognostic information in breast carcinoma. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205536
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http://dx.doi.org/10.1136/jclinpath-2018-205536DOI Listing
January 2019
8 Reads

Molecular characterisation of haemoglobin E-Udon Thani (HBB:c.[79G>A;92+7A>G]): a novel form of Hb E-β-thalassaemia syndrome.

J Clin Pathol 2019 Jan 10. Epub 2019 Jan 10.

Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand

Interaction of β-globin gene with an in trans β-thalassaemia gene leads to thalassaemia syndrome, known as haemoglobin (Hb) E-β-thalassaemia disease, with variable clinical and haematological severity. Here, we reported for the first time the Hb E-β-thalassaemia syndrome caused alternatively by an in cis interaction of β and a novel IVSI#7;A>G mutation, namely the Hb E-Udon Thani (HBB:c.[79G>A;92+7 A>G]). Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205525
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http://dx.doi.org/10.1136/jclinpath-2018-205525DOI Listing
January 2019
7 Reads

Stromal morphological changes and immunophenotypic features of precancerous lesions and hepatocellular carcinoma.

J Clin Pathol 2019 Jan 4. Epub 2019 Jan 4.

Department of Hepatobiliary Surgery, Peking University Organ Transplantation Institute, Peking University People's Hospital, Beijing, China

Aims: To evaluate stromal histopathological features and immunostaining expression for differential diagnosis of low- and high-grade dysplastic nodules (HGDN) to early and progressed hepatocellular carcinomas (eHCC, pHCC).

Materials: We evaluated sinusoid capillarisation (SC), solitary artery (SA), ductular reaction (DR), stromal invasion and expression of six biomarkers (GPC3, HSP70, GS, CD34, CK19, EpCAM) in a series of 97 cases.

Results: Stromal morphological changes, including SC, DR and SA, exhibited significant differences in differential diagnosis. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205611DOI Listing
January 2019

Relative criticalness of common laboratory tests for critical value reporting.

J Clin Pathol 2018 Dec 28. Epub 2018 Dec 28.

Biomedical Institution for Global Health and Technology, National University of Singapore, Singapore, Singapore

We determined the relative strengths of association between 23 most commonly ordered laboratory tests and the adverse outcome as indicators of relative criticalness. The lowest and highest results for 23 most commonly ordered laboratory tests, 24 hours prior to death during critical care unit (CCU) stay or discharge from CCU were extracted from a publicly available CCU database (Medical Information Mart for Intensive Care-III). Following this, the Random Forest model was applied to assess the association between the laboratory results and the outcomes (death or discharge). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205549DOI Listing
December 2018

Neoadjuvant chemoradiation and rectal cancer.

J Clin Pathol 2019 Feb 28;72(2):97-101. Epub 2018 Dec 28.

Laboratory Medicine Program, Department of Anatomical Pathology, University Health Network and University of Toronto, Toronto, Ontario, Canada.

Neoadjuvant chemoradiation (NACR) is now standard of care in stage II and III rectal cancer. The advent of this modality of treatment has impacted on the way the pathological evaluation of resection specimens that have been subjected to preoperative chemoradiation is conducted. The gross description, sectioning and microscopic examination have had to be adapted to accommodate the changes induced by NACR. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205592
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http://dx.doi.org/10.1136/jclinpath-2018-205592DOI Listing
February 2019
1 Read

Peculiar papular eruption on the vulva and inguinal folds.

J Clin Pathol 2019 Feb 20;72(2):185. Epub 2018 Dec 20.

Department of Dermatology, Cairo University, Cairo, Egypt

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http://dx.doi.org/10.1136/jclinpath-2018-205434DOI Listing
February 2019

Novel prognostic histopathological grading system in oral squamous cell carcinoma based on tumour budding and cell nest size shows high interobserver and intraobserver concordance.

J Clin Pathol 2018 Dec 8. Epub 2018 Dec 8.

Institute of Pathology, Technical University of Munich (TUM), Munich, Germany.

Aims: Squamous cell carcinoma of the oral cavity (OSCC) is a common tumour entity with a variable, partially highly aggressive clinical course. Recently, we proposed a novel (three-tiered) clinically useful grading scheme strongly associated with patient outcome in OSCC, consisting of a sum score of the histomorphological patterns tumour budding and cell nest size which outperforms WHO based grading algorithms currently in use. The aim of our study was to probe for interobserver and intraobserver reliability of this novel grading system. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205454
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http://dx.doi.org/10.1136/jclinpath-2018-205454DOI Listing
December 2018
7 Reads

Deep learning for detecting tumour-infiltrating lymphocytes in testicular germ cell tumours.

J Clin Pathol 2019 Feb 5;72(2):157-164. Epub 2018 Dec 5.

Nuffield Department of Surgical Sciences and NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, UK.

Aims: To evaluate if a deep learning algorithm can be trained to identify tumour-infiltrating lymphocytes (TILs) in tissue samples of testicular germ cell tumours and to assess whether the TIL counts correlate with relapse status of the patient.

Methods: TILs were manually annotated in 259 tumour regions from 28 whole-slide images (WSIs) of H&E-stained tissue samples. A deep learning algorithm was trained on half of the regions and tested on the other half. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205328DOI Listing
February 2019
1 Read

Assessment of in vitro effects of direct thrombin inhibitors and activated factor X inhibitors through clot waveform analysis.

J Clin Pathol 2019 Mar 5;72(3):244-250. Epub 2018 Dec 5.

Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan.

Aims: Clot waveform analysis (CWA) has been reported to extend the interpretation of clotting time measurement. The parameters obtained from successive derivatives of the clotting reaction curves reflect the rates of activation of individual coagulation factors, theoretically dissecting the cascade pathway. This study aims to assess the in vitro effects of direct thrombin inhibitors (DTIs) and activated factor X (FXa) inhibitors. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205517DOI Listing
March 2019
1 Read

JAK2-tree: a simple CBC-based decision rule to guide appropriate JAK2 V617F mutation testing.

J Clin Pathol 2019 Feb 4;72(2):172-176. Epub 2018 Dec 4.

Division of Haematology, Calgary Lab Services & Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.

Aims: The JAK2 V617F mutation is highly recurrent in many of the myeloproliferative neoplasms, a molecular variant that can be easily detected using sensitive and minimally invasive techniques. Given the ease of JAK2 V617F testing, this test may be improperly requested for the purposes of patient 'screening' and to optimise laboratory resource utilisation, it behooves clinicians and laboratorians to perform JAK2 V617F testing only when most appropriate.

Methods: To assist with the screening of patients being considered for JAK2 V617F testing, we developed a clinical decision rule, "JAK2-tree", which can be easily applied to basic CBC parameters (haemoglobin, platelet and white blood cell counts). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205527DOI Listing
February 2019
1 Read

and DNA-repair gene polymorphisms genotyping as a low-cost lung adenocarcinoma screening tool.

J Clin Pathol 2019 Jan 22;72(1):75-80. Epub 2018 Nov 22.

Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.

Aim: and DNA repair polymorphisms have been proposed as cancer risk factors. This study evaluated the usability of Arg72Pro single-nucleotide polymorphism, Arg399Gln and G135C as a low-cost lung adenocarcinoma screening tool.

Patients And Methods: This case-control study included 78 atients with lung adenocarcinoma and 79 healthy matched controls. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205553DOI Listing
January 2019

Revitalising an academic pathology department: lessons learnt.

J Clin Pathol 2019 Mar 22;72(3):213-220. Epub 2018 Nov 22.

Department of Pathology, McGill University and MGill University Health Center, Montreal, Quebec, Canada.

Pathology is a specialty that bridges basic medical science and clinical practice. In the era of personalised medicine, this specialty is facing unprecedented challenges. Some of these challenges are institution-specific, while many are shared worldwide at different magnitude. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205516DOI Listing
March 2019
9 Reads

Distinguishing thymoma from T-lymphoblastic leukaemia/lymphoma: a case-based evaluation.

J Clin Pathol 2019 Mar 22;72(3):251-257. Epub 2018 Nov 22.

Department of Leukemia, The University of Texas, MD Anderson Cancer Center, Houston, Texas, USA

T-lymphoblastic lymphoma and thymoma are distinct primary mediastinal neoplasms that can have similar clinical presentations and overlapping histological features. Microscopic distinction is occasionally difficult because the immature lymphocytes associated with thymoma may resemble T-lymphoblastic leukaemia/lymphoma cells, morphologically and immunohistochemically. An accurate diagnosis assumes particular importance since the treatment approaches for these two entities differ considerably. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205399DOI Listing
March 2019
14 Reads

Circulating tumour DNA, microRNA and metabolites in cerebrospinal fluid as biomarkers for central nervous system malignancies.

J Clin Pathol 2018 Nov 22. Epub 2018 Nov 22.

Department of Pathology and Laboratory Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, USA

Central nervous system (CNS) malignancies can be difficult to diagnose and many do not respond satisfactorily to existing therapies. Monitoring patients with CNS malignancies for treatment response and tumour recurrence can be challenging because of the difficulty and risks of brain biopsies, and the low specificity and sensitivity of the less invasive methodologies that are currently available. Uncertainty about tumour diagnosis or whether a tumour has responded to treatment or has recurred can cause delays in therapeutic decisions that can impact patient outcome. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205414DOI Listing
November 2018
8 Reads

Recurrent exon 2 mutations in benign breast fibroepithelial lesions in adolescents and young adults.

J Clin Pathol 2019 Mar 22;72(3):258-262. Epub 2018 Nov 22.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA

Aims: Most benign breast fibroepithelial lesions (FEL) in adults harbour recurrent somatic exon 2 mutations and rare promoter hotspot mutations. We sought to determine the frequency of exon 2 and promoter hotspot mutations in fibroadenomas (FA) and benign phyllodes tumours (BePT) in adolescents and young adults.

Methods: DNA from 21 consecutive FAs and eight consecutive BePTs in adolescents and young adults was subjected to Sanger sequencing of the exon 2 of and the promoter hotspot locus. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205570DOI Listing
March 2019
12 Reads

Prognostic significance of tumour infiltration growth pattern in patients with advanced gastric cancer.

J Clin Pathol 2019 Feb 22;72(2):165-171. Epub 2018 Nov 22.

Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China

Aims: The prognostic significance of infiltration growth pattern (INF) in patients with gastric cancer (GC) remains controversial. In the present study, we evaluated the impact of INF pattern on the prognosis of patients with advanced GC.

Methods: A total of 1455 patients with advanced GC who underwent curative gastrectomy in our institution were retrospectively analysed. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205403DOI Listing
February 2019
1 Read

Modified approach to fibrinogen replacement in the setting of dysfibrinogenaemia.

J Clin Pathol 2019 Feb 21;72(2):177-180. Epub 2018 Nov 21.

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.

Most fibrinogen replacement strategies focus on quantitative deficiencies. A thrombin time (TT) mixing study helped to assess qualitative defects caused by dysfibrinogens. Plasma samples were collected from non-anticoagulated subjects (n=6) meeting laboratory criteria for suspected dysfibrinogenaemia (TT > 22 s; fibrinogen activity <180) and from a control group. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205438DOI Listing
February 2019
7 Reads

Evaluation of susceptibility of Gram-positive pathogens from a tertiary care hospital in Singapore to a novel oxazolidinone, tedizolid, by a gradient diffusion method and broth microdilution.

J Clin Pathol 2019 Feb 16;72(2):181-184. Epub 2018 Nov 16.

Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore.

We compared the in vitro antimicrobial activities of tedizolid and linezolid on the Sensititre broth microdilution system for Gram-positive cocci isolates (n=146) from skin and skin structure infections and bloodstream infections, bronchoalveolar lavage and sputum. These pathogens included 40 methicillin-resistant , 38 coagulase-negative staphylococci, 20 and 48 beta-haemolytic spp. Susceptibility was simultaneously determined for 48 vanA vancomycin-resistant enterococci isolates 2013-2016 from rectal swabs (23 and 25 , of which 4 were linezolid-non-susceptible). Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205473
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http://dx.doi.org/10.1136/jclinpath-2018-205473DOI Listing
February 2019
16 Reads

RAS genes in colorectal carcinoma: pathogenesis, testing guidelines and treatment implications.

J Clin Pathol 2019 Feb 13;72(2):135-139. Epub 2018 Nov 13.

Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, USA

The RAS family is among the most commonly mutated genes in all human malignancies including colon cancer. In normal cells, RAS proteins act as a link in the intracellular signal transduction initiated by binding of growth factors to cell membrane receptors mediating cell survival. RAS isoproteins have great morphological similarities, but despite that, they are thought to have different functions in different tissues. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205471
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http://dx.doi.org/10.1136/jclinpath-2018-205471DOI Listing
February 2019
11 Reads

Apocrine lesions of the breast: part 2 of a two-part review. Invasive apocrine carcinoma, the molecular apocrine signature and utility of immunohistochemistry in the diagnosis of apocrine lesions of the breast.

J Clin Pathol 2019 Jan 13;72(1):7-11. Epub 2018 Nov 13.

Histopathology, Breast Check, Irish National Breast Screening Programme and St. Vincent's University Hospital, Dublin, Ireland.

Pure apocrine carcinoma of the breast is rare and has been defined by using a combination of morphologic (apocrine morphology in >90% of tumour cells) and immunohistochemical criteria (oestrogen receptor (ER) and progesterone receptor (PR) negative and androgen receptor (AR) positive). Recent advances in the molecular classification of breast tumours have uncovered a subset of breast tumours associated with high expression of androgen receptor mRNA including the so-called 'luminal androgen receptor (LAR) tumours' and 'molecular apocrine tumours' (MATs). Recognition of these tumour subsets has opened potential avenues for therapies exploiting the AR pathway in triple negative breast carcinoma (TNBC). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205485DOI Listing
January 2019
2 Reads

Apocrine lesions of the breast: part 1 of a two-part review: benign, atypical and in situ apocrine proliferations of the breast.

J Clin Pathol 2019 Jan 8;72(1):1-6. Epub 2018 Nov 8.

Department of Histopathology, St Vincent's University Hospital, Dublin, Ireland.

Apocrine morphology is a common phenomenon encountered in everyday breast pathology practice, and is defined as cuboidal or columnar cells exhibiting abundant eosinophilic granular cytoplasm, prominent apical granules, a low nuclear-cytoplasmic ratio, and round nuclei with pale chromatin and prominent nucleoli. Apocrine morphology is recognised in benign, atypical and malignant lesions of the breast. The morphology of apocrine atypia and non-high-grade apocrine ductal carcinoma in situ (DCIS) is less well defined due to the relative rarity of these lesions. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205484
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http://dx.doi.org/10.1136/jclinpath-2018-205484DOI Listing
January 2019
19 Reads

Tumour heterogeneity in triplet-paired metastatic tumour tissues in metastatic renal cell carcinoma: concordance analysis of target gene sequencing data.

J Clin Pathol 2019 Feb 8;72(2):152-156. Epub 2018 Nov 8.

Department of Urology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang, South Korea

Aims: The aim of the present study was to determine the concordant correlation in the expression of 88 target genes from triple-paired metastatic tissues in individual patients with metastatic renal carcinoma (mRCC) using a target gene sequencing (TGS) approach.

Methods: Between 2002 and 2017, a total of 350 triple-paired metastatic tissue samples from 262 patients with mRCC obtained from either nephrectomy or metastasectomy were used for TGS of 88 candidate genes. After quality check, 243 tissue samples from 81 patients were finally applied to TGS. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205456
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http://dx.doi.org/10.1136/jclinpath-2018-205456DOI Listing
February 2019
7 Reads

Diagnosis of intrathyroidal ectopic thymus in thyroid fine needle aspiration samples.

J Clin Pathol 2019 Feb 1;72(2):145-151. Epub 2018 Nov 1.

Department of Radiology, Children's Mercy Hospital, Kansas City, Missouri, USA.

Aims: Intrathyroidal ectopic thymus (ITET) is a rare cause of paediatric thyroid nodules. Although ultrasonography of ITET demonstrates a characteristic appearance similar to that of normal thymus, accurate differentiation from other thyroid nodule etiologies by ultrasonography is difficult, and so that fine needle aspiration (FNA) is usually performed for further analysis. The aim of this study was to evaluate the utility of flow cytometry (FCM) in confirming the diagnosis of ITET in thyroid FNA samples. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205424DOI Listing
February 2019
2 Reads

Positive association of PIK3CA mutation with KRAS mutation but not BRAF mutation in colorectal cancer suggests co-selection is gene specific but not pathway specific.

J Clin Pathol 2019 Mar 25;72(3):263-264. Epub 2018 Oct 25.

Molecular Pathology Group, Unit of Academic Molecular Pathology, Division of Cancer and Stem Cell, School of Medicine, University of Nottingham, Queen's Medical Centre Campus, Nottingham, UK

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http://dx.doi.org/10.1136/jclinpath-2018-205483DOI Listing
March 2019
3 Reads

HbA1c: a review of non-glycaemic variables.

J Clin Pathol 2019 Jan 25;72(1):12-19. Epub 2018 Oct 25.

Department of Chemical Pathology, Western Sussex Hospitals NHS Foundation Trust, Worthing, England.

Identification of the correlation between HbA1c and diabetic complications has yielded one of the most clinically useful biomarkers. HbA1c has revolutionised the diagnosis and monitoring of diabetes mellitus. However, with widespread adoption of HbA1c has come increasing recognition that non-glycaemic variables can also affect HbA1c, with varying clinical significance. Read More

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http://dx.doi.org/10.1136/jclinpath-2017-204755DOI Listing
January 2019
2 Reads

Diagnostic predictive value of Xpert Bladder Cancer Monitor in the follow-up of patients affected by non-muscle invasive bladder cancer.

J Clin Pathol 2019 Feb 24;72(2):140-144. Epub 2018 Oct 24.

Department of Urology, Central Hospital of Bolzano, Bolzano, Italy.

Aims: Cystoscopy and urine cytology represent the gold standard for monitoring superficial bladder cancer (BC). Xpert BC Monitor is a new urinary marker based on the evaluation of five target mRNAs overexpressed in patients with bladder cancer. The aim of our study was to evaluate the diagnostic accuracy of Xpert BC Monitor in follow-up of patients with non-muscle invasive bladder cancer (NMIBC). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205393DOI Listing
February 2019
3 Reads

Benign notochordal cell tumour: clinicopathology and molecular profiling of 13 cases.

J Clin Pathol 2019 Jan 24;72(1):66-74. Epub 2018 Oct 24.

Department of Neuropathology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, China National Clinical Research Center for Neurological Diseases (NCRC-ND), Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing Key Laboratory of Brain Tumor, Beijing, China

Aims: To study the clinicopathological and molecular features of benign notochordal cell tumours (BNCTs) and their differential diagnosis from chordoma.

Methods: 13 cases of BNCT were investigated. The genome-wide copy number imbalances were performed using Oncoscan CNV array in three cases and fluorescence in situ hybridisation (FISH) detection of epidermal growth factor receptor (EGFR)/chromosome 7 enumeration probe (CEP7), LSI1p36/1q21, LSI19p13/19q13, CEP3/CEP12 and Telvysion 6 P was performed in 13 cases. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205441DOI Listing
January 2019
3 Reads

Fat clearance of upper gastrointestinal resection specimens increases lymph node yield and may result in nodal upstaging.

J Clin Pathol 2019 Jan 23;72(1):86-89. Epub 2018 Oct 23.

Department of Histopathology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK.

Introduction: Lymph node retrieval and quantification is an important element in staging upper gastrointestinal cancers. Our department introduced fat clearance for oesophagectomy and gastrectomy specimens in 2014. This study assessed the impact of this change on lymph node yield and upstaging. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205457
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http://dx.doi.org/10.1136/jclinpath-2018-205457DOI Listing
January 2019
11 Reads

Feasibility and safety of targeting the anterior superior iliac spine to perform a bone marrow procedure: a prospective, clinical study.

J Clin Pathol 2018 Dec 23;71(12):1116-1119. Epub 2018 Oct 23.

Division of Hematology, Department of Medicine, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA.

Aims: The bone marrow procedure (BMP) has been performed worldwide for years. Nonetheless, no generally accepted standards or guidelines for the performance of the BMP exist. Recent studies suggested that the lateral angulation technique (LAT), targeting the anterior superior iliac spine (ASIS) after penetration of the posterior superior iliac spine, yields longer biopsy cores and is safer for patients. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205309
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http://dx.doi.org/10.1136/jclinpath-2018-205309DOI Listing
December 2018
7 Reads