16,205 results match your criteria Journal of clinical pathology[Journal]


C-reactive protein and neutrophil count laboratory test requests from primary care: what is the demand and would substitution by point-of-care technology be viable?

J Clin Pathol 2019 Apr 16. Epub 2019 Apr 16.

Nuffield Department of Primary Care Health Sciences, University of Oxford, Oxford, UK.

AIMS : C-reactive protein (CRP) and neutrophil count (NC) are important diagnostic indicators of inflammation. Point-of-care (POC) technologies for these markers are available but rarely used in community settings in the UK. To inform the potential for POC tests, it is necessary to understand the demand for testing. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205688DOI Listing

Machine learning algorithms for the detection of spurious white blood cell differentials due to erythrocyte lysis resistance.

J Clin Pathol 2019 Apr 16. Epub 2019 Apr 16.

Experimental and Health Sciences, Pompeu Fabra University, Barcelona, Spain

Aims: Red blood cell (RBC) lysis resistance interferes with white blood cell (WBC) count and differential; still, its detection relies on the identification of an abnormal scattergram, and this is not clearly adverted by specific flags in the Beckman-Coulter DXH-800. The aims were to analyse precisely the effect of RBC lysis resistance interference in WBC counts, differentials and cell population data (CPD) and then to design, develop and implement a novel diagnostic machine learning (ML) model to optimise the detection of samples presenting this phenomenon.

Methods: WBC counts, differentials and CPD from 232 patients (anaemia or liver disease) were compared with 100 healthy controls (HC) using analysis of variance. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2019-205820
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http://dx.doi.org/10.1136/jclinpath-2019-205820DOI Listing
April 2019
1 Read

Fibrinolysis during liver transplantation: analysis by the Thrombodynamics method.

J Clin Pathol 2019 Apr 11. Epub 2019 Apr 11.

Laboratoire d'hématologie hôpital Pellegrin- PTRR, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.

An issue in orthotopic liver transplantation (OLT) is the diagnosis of hyperfibrinolysis. The Thrombodynamics-4D assay (TD4D) is a videomicroscopy system allowing the dynamic analysis of fibrin clot. Fibrinolysis is highlighted by a change in clot intensity. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205560DOI Listing

Improving the specific diagnosis of trematode, cestode and nematode infections by a multiplex single-tube real-time PCR assay.

J Clin Pathol 2019 Apr 5. Epub 2019 Apr 5.

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Aims: Helminth infections are becoming uncommon in high-income countries and laboratory staff may lose expertise in their morphological identification, especially in histological sections where speciation of helminths is challenging. Commercially available molecular diagnostic panels for faecal specimens only offer tests for protozoa but not helminths. We aim to improve the identification accuracy of helminths using a multiplex PCR assay. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205590DOI Listing
April 2019
1 Read

First evidence of a paediatric patient with Cornelia de Lange syndrome with acute lymphoblastic leukaemia.

J Clin Pathol 2019 Apr 4. Epub 2019 Apr 4.

Centro di Ricerca Tettamanti, Clinica Pediatrica, Università di Milano, Bicocca, Monza, Italy

Cornelia de Lange syndrome (CdLS) is a rare autosomal-dominant genetic disorder characterised by prenatal and postnatal growth and mental retardation, facial dysmorphism and upper limb abnormalities. Germline mutations of cohesin complex genes , , or their regulators and have been identified in CdLS as well as somatic mutations in myeloid disorders. We describe the first case of a paediatric patient with CdLS with B-cell precursor Acute Lymphoblastic Leukaemia (ALL). Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205707DOI Listing
April 2019
2 Reads

First family case of haemoglobinopathy Titusville in France and literature overview.

J Clin Pathol 2019 Apr 2. Epub 2019 Apr 2.

Clinical Chemistry Laboratory, University Hospital of Nice, Nice, France.

Normal haemoglobin is a tetramer molecule, consisting of two α and β haemoglobin chains. Haemoglobinopathies occur when abnormalities in these proteins are present. More than 1000 naturally occurring human haemoglobin variants with single amino acid substitution throughout the molecule have been identified and can be discovered through their clinical and biological manifestations. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205616DOI Listing

Red cell adenylate kinase deficiency in India: identification of two novel missense mutations (c.71A>G and c.413G>A).

J Clin Pathol 2019 Mar 27. Epub 2019 Mar 27.

Department of Haematogenetics, National Institute of Immunohaematology, Mumbai, India

Adenylate kinase (AK) deficiency is a rare erythroenzymopathy associated with hereditary nonspherocytic haemolytic anaemia along with mental/psychomotor retardation in few cases. Diagnosis of AK deficiency depends on the decreased level of enzyme activity in red cell and identification of a mutation in the gene. Until, only eight mutations causing AK deficiency have been reported in the literature. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205718DOI Listing
March 2019
1 Read

Expression of LEF1 and TCF1 (TCF7) proteins associates with clinical progression of nasopharyngeal carcinoma.

J Clin Pathol 2019 Mar 27. Epub 2019 Mar 27.

Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, China

Aims: Our previous study has demonstrated that β-catenin pathway was abnormally activated in nasopharyngeal carcinoma (NPC). The purposes of the present study are to investigate whether the alterations of LEF1 and TCF1 (TCF7) proteins, the important components of the canonical Wnt/β-catenin pathway, are associated with clinicopathological features and prognostic implications.

Methods: We collected 391 cases of NPC, 53 non-cancerous control nasopharyngeal mucosa and 28 pairs of NPC and their matched metastases, detected expression of LEF1 and TCF1 (TCF7) proteins in these tissues by immunohistochemistry. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205698DOI Listing

Hyperammonaemia following exercise may also reveal PGK1 deficiency.

J Clin Pathol 2019 Mar 27. Epub 2019 Mar 27.

Paris-Est Neuromuscular Center, Institute of Myology, University Hospital Pitié Salpêtrière, Paris, France.

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http://dx.doi.org/10.1136/jclinpath-2019-205750DOI Listing

Survey of UK histopathology consultants' attitudes towards academic and molecular pathology.

J Clin Pathol 2019 Mar 25. Epub 2019 Mar 25.

Academic Unit of Oral and Maxillofacial Medicine, Pathology and Surgery, University of Sheffield, Sheffield, UK.

Objective: Academic pathology is facing a crisis; an ongoing decline in academic pathology posts, a paucity of academic pathologist's in-training and unfilled posts at a time when cellular pathology departments are challenged to deliver increasing numbers of molecular tests. The National Cancer Research Institute initiative in Cellular & Molecular Pathology commissioned a survey to assess attitudes of cellular pathology consultants towards research in order to understand barriers and identify possible solutions to improve this situation. As cellular pathology is encompassing an increasing number of diagnostic molecular tests, we also surveyed the current approach to and extent of training in molecular pathology. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205568DOI Listing
March 2019
4 Reads

Impact and importance of a centralised review panel for lymphoma diagnostics in the WHO era: a single-centre experience.

J Clin Pathol 2019 Mar 25. Epub 2019 Mar 25.

Department of Histopathology, St James's Hospital, Dublin, Ireland.

Lymphoma diagnosis is complex, requiring a wide array of adjunctive tests to reach accurate diagnoses. We retrospectively examined the rates of concordance between referral and review lymphoma diagnoses on cases referred to St James's Hospital, Dublin for multidisciplinary team review between 2013 and 2016. Frequency and cost of adjunctive diagnostic tests performed were also analysed. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205691DOI Listing

Selective screening of late-onset Pompe disease (LOPD) in patients with non-diagnostic muscle biopsies.

J Clin Pathol 2019 Mar 16. Epub 2019 Mar 16.

Division of Neurology, Institute of Clinical Neurophysiology, University Medical Centre Ljubljana, Ljubljana, Slovenia.

Aims: As of 2016, there were five patients with Pompe in Slovenia (two infantile, one childhood and two adult onset) with a prevalence of 1:400 000; however, the prevalence of late-onset Pompe disease (LOPD) in some other countries means this ratio could be an underestimate. Since an LOPD muscle biopsy could be unspecific or even normal, the purpose of this study is to assess the prevalence of LOPD in patients with non-diagnostic muscle biopsies.

Methods: Six hundred biopsies were recorded at the Neuromuscular Tissue Bank of the University of Ljubljana for the period 2004-2014. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205446DOI Listing

Comparison of methodologies for the detection of mutations in bone marrow trephine specimens.

J Clin Pathol 2019 Mar 14. Epub 2019 Mar 14.

Nuffield Division of Clinical Laboratory Sciences, University of Oxford, Oxford, UK.

Aims: V600E detection assists in the diagnosis of hairy cell leukaemia (HCL); however, testing practices vary. We evaluated the clinical utility of 5 mutation testing strategies for use on bone marrow trephines (BMT).

Methods: 11 HCL, 5 HCL 'mimic', 2 treated HCL and 10 normal BMT specimens were tested for mutant comparing Sanger sequencing, pyrosequencing, amplicon-based next generation sequencing (NGS), automated (Idylla) PCR and immunohistochemistry (IHC). Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205734DOI Listing
March 2019
1 Read

Comparative prognostic analysis for triple-negative breast cancer with metaplastic and invasive ductal carcinoma.

J Clin Pathol 2019 Mar 14. Epub 2019 Mar 14.

Pathology Tissue Bank, Qilu Hospital of Shandong University, Jinan, Shandong, China

Aims: Triple-negative breast cancer comprises different histological subtypes, including metaplastic breast cancer (MBC) and ductal carcinomas (IDCs). The purpose of this study was to compare triple-negative MBC (TN-MBC) with triple-negative IDC (TN-IDC) in terms of survival and predictive factors.

Methods: With access to the Surveillance, Epidemiology and End Result (SEER) database, a total of 19 383 patients met the eligibility criteria. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205544DOI Listing
March 2019
1 Read

Measuring too much or too little in adult coeliac disease.

J Clin Pathol 2019 May 14;72(5):341-342. Epub 2019 Mar 14.

First Department of Internal Medicine, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy

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http://dx.doi.org/10.1136/jclinpath-2018-205667DOI Listing
May 2019
2.915 Impact Factor

PD-L1 expression on routine samples of non-small cell lung cancer: results and critical issues from a 1-year experience of a centralised laboratory.

J Clin Pathol 2019 Mar 7. Epub 2019 Mar 7.

Department of Public Health, University of Naples 'Federico II', Naples, Italy.

Aims: Our laboratory is a centralised centre receiving routine non-small cell lung cancer (NSCLC) samples for programmed death ligand-1 (PD-L1) immunohistochemical (IHC) evaluation. Since literature data are not concordant here we review our clinical records to assess the rate of PD-L1 positive and negative NSCLC cases in real-world practice.

Methods: PD-L1 expression was evaluated by a validated 22C3 IHC laboratory developed test on 211 prospectively collected routine NSCLC samples, received from 10 outside institutions. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205732DOI Listing
March 2019
1 Read

High kinesin family member 11 expression predicts poor prognosis in patients with clear cell renal cell carcinoma.

J Clin Pathol 2019 May 28;72(5):354-362. Epub 2019 Feb 28.

State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Biological Sciences, Inner Mongolia University, Inner Mongolia, China

Aims: Kinesin family member 11 (Kif11) is a member of the kinesin family motor proteins, which is associated with spindle formation and tumour genesis. In this study, we investigated the relationship between Kif11 expression and clear cell renal cell carcinoma (CCRCC) development.

Methods: The relationship between Kif11 expression and CCRCC development was analysed by quantitative real-time (qRT)-PCR analyses, and tissue immunohistochemistry. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205390DOI Listing
May 2019
1 Read

Transformation of a splenic marginal zone lymphoma into classic Hodgkin lymphoma.

J Clin Pathol 2019 May 27;72(5):391-392. Epub 2019 Feb 27.

Institute of Pathology, University Hospital Basel, Basel, Switzerland

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http://dx.doi.org/10.1136/jclinpath-2018-205678DOI Listing
May 2019
1 Read

Detection of mutations using target capture sequencing in plasma of patients with non-small-cell lung cancer.

J Clin Pathol 2019 May 20;72(5):379-385. Epub 2019 Feb 20.

Department of Respiratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China

PURPOSE : Circulating tumour DNA (ctDNA) is a promising biomarker for detection of non-invasive epidermal growth factor receptor ) mutations in patients with non-small-cell lung cancer (NSCLC). However, the existing methods have limitations in sensitivity or in availability. The aim was to evaluate the accuracy of capture target sequencing for detecting mutations in ctDNA. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205699DOI Listing

Agar pre-embedding of small skin biopsies: real-life benefits and challenges in high throughput pathology laboratories.

J Clin Pathol 2019 Feb 20. Epub 2019 Feb 20.

Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), Univeristy of Genoa, Genoa, Italy

Paraffin embedding of small, thin tissue samples requires specific expertise for optimal orientation before tissue sectioning. This study evaluates the real-life utility of the agar pre-embedding technique for small skin biopsies with regards to lengthening of work times, problems in orientation (re-embedding) and ancillary techniques (immunohistochemistry and in situ hybridisation) between two high work flow pathology laboratories, one of which routinely uses the agar pre-embedding technique and one which does not. The mean time required for pre-embedding in agar was 30. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205680DOI Listing
February 2019

The adaptation of AABACUS for quality improvement in laboratory workflow analysis ('L-AABACUS').

J Clin Pathol 2019 Mar;72(3):237-243

Laboratory Medicine Program, University Health Network, Toronto, Canada

The ability to effectively monitor key indicators is important for continuous quality improvement in laboratory immunohistochemistry. This article deals specifically with laboratory turnaround time (TAT) as a key delivery indicator and the impact of laboratory workflow on laboratory TATs. While our laboratory has traditionally relied on the manual calculation of slide-TAT (S-TAT) to monitor delivery, we have determined that automated calculation of case-TAT (C-TAT) would be superior as a delivery indicator. Read More

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http://dx.doi.org/10.1136/jclinpath-2019-205725DOI Listing

Digital immunohistochemistry implementation, training and validation: experience and technical notes from a large clinical laboratory.

J Clin Pathol 2019 May 14;72(5):373-378. Epub 2019 Feb 14.

Histopathology Department, Bexley Wing, St James University Hospital, Leeds, UK.

Aims: To consider the value proposition of digitisation of clinical immunohistochemistry services, and to develop an approach to digital immunohistochemistry implementation and validation in a large clinical laboratory.

Methods: A methodology for slide scanning in the laboratory was developed, in addition to a novel validation exercise, to allow pathologists to identify the strengths and weaknesses of digital immunohistochemistry reporting, and train in digital immunohistochemistry slide assessment.

Results: A total of 1480 digital immunohistochemistry slides were assessed by 24 consultant pathologists, with complete clinical concordance between the digital and the glass slide assessment observed. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205628DOI Listing
May 2019
1 Read

Detection of free intraperitoneal tumour cells in peritoneal lavage fluid from patients with peritoneal metastasis before and after treatment with pressurised intraperitoneal aerosol chemotherapy (PIPAC).

J Clin Pathol 2019 May 12;72(5):368-372. Epub 2019 Feb 12.

Odense PIPAC Center, Odense University Hospital, Odense, Denmark.

Aims: In this study, we investigated whether free intraperitoneal tumour cells (FITC) were detectable in ascites or peritoneal lavage fluid (PLF) from patients with peritoneal metastasis (PM) before and after treatment with pressurised intraperitoneal aerosol chemotherapy (PIPAC).

Methods: Ascites or PLF retrieved at the first and third PIPAC procedures was analysed by conventional cytology, carcinoembryonic antigen (CEA) and total protein concentration, and quantitative reverse transcriptase PCR (qRT-PCR) for mRNA expression of CEA, epithelial cell adhesion molecule (EpCAM) and cancer antigen 125 (CA-125). Conventional cytology and qRT-PCR were also performed in a negative control group (benign PLF specimens and inflammatory ascites). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205683DOI Listing
May 2019
1 Read

Nuclear factor erythroid 2-related factors 1 and 2 are able to define the worst prognosis group among high-risk diffuse large B cell lymphomas treated with R-CHOEP.

J Clin Pathol 2019 Apr 12;72(4):316-321. Epub 2019 Feb 12.

Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.

Aims: Oxidative stress markers and antioxidant enzymes have previously been shown to have prognostic value and associate with adverse outcome in patients with diffuse large B cell lymphoma (DLBCL). Nuclear factor erythroid 2-related factor 1 (Nrf1) and factor 2 (Nrf2) are among the principal inducers of antioxidant enzyme production. Kelch ECH associating protein 1 (Keap1) is a negative regulator of Nrf2, and BTB (BR-C, ttk and bab) domain and CNC homolog 1 (Bach1) represses the function of both factors. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205584DOI Listing
April 2019
3 Reads

Accuracy of renal tumour biopsy for the diagnosis and subtyping of papillary renal cell carcinoma: analysis of paired biopsy and nephrectomy specimens with focus on discordant cases.

J Clin Pathol 2019 May 12;72(5):363-367. Epub 2019 Feb 12.

Department of Pathology, University Health Network, Toronto, Ontario, Canada.

Aims: Renal tumour biopsy (RTB) is increasingly recognised as a useful diagnostic tool in the management of small renal masses, particularly those that are incidentally found. Intratumoural heterogeneity with respect to morphology, grade and molecular features represents a frequently identified limitation to the use of RTB. While previous studies have evaluated pathological correlation between RTB and nephrectomy, no studies to date have focused specifically on the role of RTB for the diagnosis of papillary renal cell carcinoma (PRCC) and its further subclassification into clinically relevant subtypes. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205655
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http://dx.doi.org/10.1136/jclinpath-2018-205655DOI Listing
May 2019
9 Reads

Benign spindle cell lesions of the breast: a diagnostic approach to solitary fibrous tumour, nodular pseudoangiomatous stromal hyperplasia and nodular fasciitis.

J Clin Pathol 2019 Feb 9. Epub 2019 Feb 9.

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada

Benign spindle cell lesions of the breast include neoplastic and reactive entities that are diagnostically challenging given their rarity and similar histomorphology. Accurate diagnosis on percutaneous core biopsy within this category is essential as some lesions require excision and surveillance, whereas others may be observed. We present three cases of rare benign spindle cell lesions of the breast that reflect the diversity of this group: solitary fibrous tumour, nodular pseudoangiomatous stromal hyperplasia and nodular fasciitis. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205561DOI Listing
February 2019
3 Reads

Histopathological evaluation of scleritis.

J Clin Pathol 2019 May 5;72(5):386-390. Epub 2019 Feb 5.

Department of Ophthalmology, USF Health Morsani College of Medicine, Tampa, Florida, USA

The sclera is an uncommon site of primary inflammation. Biopsy is infrequently employed in the evaluation of scleritis, but familiarity with its differential diagnosis is instrumental in ensuring efficient histological evaluation. This review provides a clinical overview of scleritis and describes the context in which scleral biopsy might arise. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205360DOI Listing

Additional loss of MSH2 and MSH6 expression in sporadic deficient mismatch repair colorectal cancer due to MLH1 promoter hypermethylation.

J Clin Pathol 2019 Feb 5. Epub 2019 Feb 5.

Pathology, University of Leeds, Leeds, UK

Colorectal cancer (CRC) is common with 3% of cases associated with germline mutations in the mismatch repair pathway characteristic of Lynch syndrome (LS). The UK National Institute for Health and Care Excellence recommends screening for LS in all patients newly diagnosed with CRC, irrespective of age. The Yorkshire Cancer Research Bowel Cancer Improvement Programme includes a regional LS screening service for all new diagnoses of CRC. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205687
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http://dx.doi.org/10.1136/jclinpath-2018-205687DOI Listing
February 2019
6 Reads

Relevance of TP53 for CLL diagnostics.

J Clin Pathol 2019 May 2;72(5):343-346. Epub 2019 Feb 2.

Department of Haematology, Beaumont Hospital, Dublin, Ireland.

disruption in chronic lymphocytic leukaemia (CLL) is a well-established prognostic marker and informs on the appropriate course of treatment for patients. status is commonly assessed by fluorescence in situ hybridisation for del(17 p) and Sanger sequencing for mutations. At present, current screening methods for mutations fail to detect diagnostically relevant mutations potentially leading to inappropriate treatment decisions. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205622DOI Listing
May 2019
1 Read

gene: its function and dysregulation in cancer.

J Clin Pathol 2019 Apr 29;72(4):281-284. Epub 2019 Jan 29.

Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Sydney, Australia.

In this review, we summarise the function and structure of telomerase reverse transcriptase () in humans, including its regulation. The dysregulation of telomerase through promoter mutations across a range of cancers is discussed. The molecular mechanism activated by promoter mutations is outlined. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205653
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http://dx.doi.org/10.1136/jclinpath-2018-205653DOI Listing
April 2019
9 Reads

International Endocervical Adenocarcinoma Criteria and Classification (IECC): correlation with adverse clinicopathological features and patient outcome.

J Clin Pathol 2019 May 24;72(5):347-353. Epub 2019 Jan 24.

Department of Laboratory Medicine and Molecular Diagnostics, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada

Aims: The International Endocervical Adenocarcinoma Criteria and Classification (IECC) was recently proposed as an improved method for categorising endocervical adenocarcinoma (EA) into human papillomavirus (HPV)-associated adenocarcinomas (HPVAs) and non-HPV-associated adenocarcinomas (NHPVAs). Such categorisation correlates with patient age and tumour size; however, its association with patient outcome remains to be established.

Methods: Institutional cases of EA with histological material available were selected. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205632DOI Listing
May 2019
2 Reads

Neoadjuvant chemoradiation in locally advanced rectal cancer: the surgeon's perspective.

J Clin Pathol 2019 Feb;72(2):133-134

Division of General Surgery, University of Toronto, Toronto, Ontario, Canada

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http://dx.doi.org/10.1136/jclinpath-2018-205595DOI Listing
February 2019

Breast specimen handling and reporting in the post-neoadjuvant setting: challenges and advances.

J Clin Pathol 2019 Feb;72(2):120-132

Laboratory Medicine Program, University Health Network, Toronto, Ontario, Canada

Neoadjuvant systemic therapy is becoming more commonly used in patients with earlier stages of breast cancer. To assess tumour response to neoadjuvant chemotherapy, pathological evaluation is the gold standard. Depending on the treatment response, the pathological examination of these specimens can be quite challenging. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205598DOI Listing
February 2019
12 Reads

Gross and microscopic changes of liver neoplasms and background hepatic structures following neoadjuvant therapy.

J Clin Pathol 2019 Feb;72(2):112-119

Department of Pathobiology and Laboratory Medicine, The University of Toronto, Toronto, Ontario, Canada

Liver transplantation is a surgical option with curative intent used in the management of some cases of hepatocellular carcinoma and cholangiocarcinoma (hilar, rarely intrahepatic). A number of different therapeutic modalities including ablative techniques, arterially directed therapies, radiation and chemotherapy are used in the neoadjuvant setting prior to liver transplantation with the goals of preventing tumour progression, decreasing post-transplant recurrence and possibly downstaging patients with tumour burden beyond what is acceptable by current transplant criteria. Pathologists evaluating hepatic explants must be aware of these neoadjuvant therapies and the alterations induced by them in both tumourous and non-tumourous tissue. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205596DOI Listing
February 2019
1 Read

Neoadjuvant therapy in gynaecological malignancies: What pathologists need to know.

J Clin Pathol 2019 Feb;72(2):102-111

Department of Anatomical Pathology, Laboratory Medicine Program, University Health Network, Toronto, Ontario, Canada.

In recent times, there has been a growing tendency to treat advanced gynaecological malignancies with neoadjuvant chemotherapy (NACT), with the goal of reducing tumour volume and enhancing operability resulting in optimal cytoreduction. This approach is used in particular for patients with advanced high-grade serous carcinoma of the ovary, fallopian tube or peritoneum. Pathology plays a crucial role in the management of these patients, both before and after NACT. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205634DOI Listing
February 2019
1 Read

Postmortem microbiology sampling following death in hospital: an ESGFOR task force consensus statement.

J Clin Pathol 2019 May 19;72(5):329-336. Epub 2019 Jan 19.

Microbiology Laboratory, Biology Department, Instituto Nacional de Toxicología y Ciencias Forenses, Las Rozas, Spain.

Postmortem microbiology (PMM) is a valuable tool in the identification of the cause of death and of factors contributory to death where death has been caused by infection. The value of PMM is dependent on careful autopsy planning, appropriate sampling, minimisation of postmortem bacterial translocation and avoidance of sample contamination. Interpretation of PMM results requires careful consideration in light of the clinical history, macroscopic findings and the histological appearances of the tissues. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205365DOI Listing

Neurotrophic tropomyosin or tyrosine receptor kinase (NTRK) genes.

Authors:
Runjan Chetty

J Clin Pathol 2019 Mar 13;72(3):187-190. Epub 2019 Jan 13.

Department of Anatomical Pathology, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada

The neurotrophic tropomyosin or tyrosine receptor kinase (NTRK) genes (1-3) are proto-oncogenes that when activated are encountered in a wide array of tumours. The recent advent of very specific and selective inhibitors of their gene fusions makes the NRTK gene fusions actionable. NRTK gene fusions are very characteristic of specific tumours: salivary mammary analogue secretory carcinoma, breast secretory carcinoma, infantile fibrosarcoma and congenital mesoblastic nephroma. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205672DOI Listing

Update on eighth edition American Joint Committee on Cancer classification for Merkel cell carcinoma and histopathological parameters that determine prognosis.

J Clin Pathol 2019 May 13;72(5):337-340. Epub 2019 Jan 13.

Department of Pathology, Dermatopathology Section, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA

Merkel cell carcinoma (MCC) is a rare primary cutaneous neuroendocrine carcinoma. The annual incidence of MCC is increasing in the USA. Timely diagnosis and proper staging of this tumour are crucial as MCC has high rates of regional recurrence and lymph node and distant metastasis. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205504
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http://dx.doi.org/10.1136/jclinpath-2018-205504DOI Listing
May 2019
10 Reads

Microvessel density in breast cancer: the impact of field area on prognostic informativeness.

J Clin Pathol 2019 Apr 10;72(4):304-310. Epub 2019 Jan 10.

Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.

Aims: Tumour microvessel density (MVD) is assessed by counting vessels in the most vascularised tumour region, the vascular hot spot. Current uncertainty regarding the prognostic role of MVD in breast cancer could, in part, be explained by variations in field area size for MVD assessment. We aimed to identify the field area size that provides the most accurate prognostic information in breast carcinoma. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205536
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http://dx.doi.org/10.1136/jclinpath-2018-205536DOI Listing
April 2019
14 Reads

Molecular characterisation of haemoglobin E-Udon Thani (HBB:c.[79G>A;92+7A>G]): a novel form of Hb E-β-thalassaemia syndrome.

J Clin Pathol 2019 Apr 10;72(4):322-324. Epub 2019 Jan 10.

Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand

Interaction of β-globin gene with an in trans β-thalassaemia gene leads to thalassaemia syndrome, known as haemoglobin (Hb) E-β-thalassaemia disease, with variable clinical and haematological severity. Here, we reported for the first time the Hb E-β-thalassaemia syndrome caused alternatively by an in cis interaction of β and a novel IVSI#7;A>G mutation, namely the Hb E-Udon Thani (HBB:c.[79G>A;92+7 A>G]). Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205525
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http://dx.doi.org/10.1136/jclinpath-2018-205525DOI Listing
April 2019
9 Reads

Stromal morphological changes and immunophenotypic features of precancerous lesions and hepatocellular carcinoma.

J Clin Pathol 2019 Apr 4;72(4):295-303. Epub 2019 Jan 4.

Department of Hepatobiliary Surgery, Peking University Organ Transplantation Institute, Peking University People's Hospital, Beijing, China

Aims: To evaluate stromal histopathological features and immunostaining expression for differential diagnosis of low- and high-grade dysplastic nodules (HGDN) to early and progressed hepatocellular carcinomas (eHCC, pHCC).

Materials: We evaluated sinusoid capillarisation (SC), solitary artery (SA), ductular reaction (DR), stromal invasion and expression of six biomarkers (GPC3, HSP70, GS, CD34, CK19, EpCAM) in a series of 97 cases.

Results: Stromal morphological changes, including SC, DR and SA, exhibited significant differences in differential diagnosis. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205611DOI Listing
April 2019
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Relative criticalness of common laboratory tests for critical value reporting.

J Clin Pathol 2019 Apr 28;72(4):325-328. Epub 2018 Dec 28.

Biomedical Institution for Global Health and Technology, National University of Singapore, Singapore, Singapore

We determined the relative strengths of association between 23 most commonly ordered laboratory tests and the adverse outcome as indicators of relative criticalness. The lowest and highest results for 23 most commonly ordered laboratory tests, 24 hours prior to death during critical care unit (CCU) stay or discharge from CCU were extracted from a publicly available CCU database (Medical Information Mart for Intensive Care-III). Following this, the Random Forest model was applied to assess the association between the laboratory results and the outcomes (death or discharge). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205549DOI Listing
April 2019
10 Reads

Neoadjuvant chemoradiation and rectal cancer.

J Clin Pathol 2019 Feb 28;72(2):97-101. Epub 2018 Dec 28.

Laboratory Medicine Program, Department of Anatomical Pathology, University Health Network and University of Toronto, Toronto, Ontario, Canada.

Neoadjuvant chemoradiation (NACR) is now standard of care in stage II and III rectal cancer. The advent of this modality of treatment has impacted on the way the pathological evaluation of resection specimens that have been subjected to preoperative chemoradiation is conducted. The gross description, sectioning and microscopic examination have had to be adapted to accommodate the changes induced by NACR. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205592
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http://dx.doi.org/10.1136/jclinpath-2018-205592DOI Listing
February 2019
2 Reads

Peculiar papular eruption on the vulva and inguinal folds.

J Clin Pathol 2019 Feb 20;72(2):185. Epub 2018 Dec 20.

Department of Dermatology, Cairo University, Cairo, Egypt

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http://dx.doi.org/10.1136/jclinpath-2018-205434DOI Listing
February 2019
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Novel prognostic histopathological grading system in oral squamous cell carcinoma based on tumour budding and cell nest size shows high interobserver and intraobserver concordance.

J Clin Pathol 2019 Apr 8;72(4):285-294. Epub 2018 Dec 8.

Institute of Pathology, Technical University of Munich (TUM), Munich, Germany.

Aims: Squamous cell carcinoma of the oral cavity (OSCC) is a common tumour entity with a variable, partially highly aggressive clinical course. Recently, we proposed a novel (three-tiered) clinically useful grading scheme strongly associated with patient outcome in OSCC, consisting of a sum score of the histomorphological patterns tumour budding and cell nest size which outperforms WHO based grading algorithms currently in use. The aim of our study was to probe for interobserver and intraobserver reliability of this novel grading system. Read More

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http://jcp.bmj.com/lookup/doi/10.1136/jclinpath-2018-205454
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http://dx.doi.org/10.1136/jclinpath-2018-205454DOI Listing
April 2019
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Deep learning for detecting tumour-infiltrating lymphocytes in testicular germ cell tumours.

J Clin Pathol 2019 Feb 5;72(2):157-164. Epub 2018 Dec 5.

Nuffield Department of Surgical Sciences and NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, UK.

Aims: To evaluate if a deep learning algorithm can be trained to identify tumour-infiltrating lymphocytes (TILs) in tissue samples of testicular germ cell tumours and to assess whether the TIL counts correlate with relapse status of the patient.

Methods: TILs were manually annotated in 259 tumour regions from 28 whole-slide images (WSIs) of H&E-stained tissue samples. A deep learning algorithm was trained on half of the regions and tested on the other half. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205328DOI Listing
February 2019
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Assessment of in vitro effects of direct thrombin inhibitors and activated factor X inhibitors through clot waveform analysis.

J Clin Pathol 2019 Mar 5;72(3):244-250. Epub 2018 Dec 5.

Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan.

Aims: Clot waveform analysis (CWA) has been reported to extend the interpretation of clotting time measurement. The parameters obtained from successive derivatives of the clotting reaction curves reflect the rates of activation of individual coagulation factors, theoretically dissecting the cascade pathway. This study aims to assess the in vitro effects of direct thrombin inhibitors (DTIs) and activated factor X (FXa) inhibitors. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205517DOI Listing
March 2019
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JAK2-tree: a simple CBC-based decision rule to guide appropriate JAK2 V617F mutation testing.

J Clin Pathol 2019 Feb 4;72(2):172-176. Epub 2018 Dec 4.

Division of Haematology, Calgary Lab Services & Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.

Aims: The JAK2 V617F mutation is highly recurrent in many of the myeloproliferative neoplasms, a molecular variant that can be easily detected using sensitive and minimally invasive techniques. Given the ease of JAK2 V617F testing, this test may be improperly requested for the purposes of patient 'screening' and to optimise laboratory resource utilisation, it behooves clinicians and laboratorians to perform JAK2 V617F testing only when most appropriate.

Methods: To assist with the screening of patients being considered for JAK2 V617F testing, we developed a clinical decision rule, "JAK2-tree", which can be easily applied to basic CBC parameters (haemoglobin, platelet and white blood cell counts). Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388913PMC
February 2019
2 Reads

and DNA-repair gene polymorphisms genotyping as a low-cost lung adenocarcinoma screening tool.

J Clin Pathol 2019 Jan 22;72(1):75-80. Epub 2018 Nov 22.

Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.

Aim: and DNA repair polymorphisms have been proposed as cancer risk factors. This study evaluated the usability of Arg72Pro single-nucleotide polymorphism, Arg399Gln and G135C as a low-cost lung adenocarcinoma screening tool.

Patients And Methods: This case-control study included 78 atients with lung adenocarcinoma and 79 healthy matched controls. Read More

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http://dx.doi.org/10.1136/jclinpath-2018-205553DOI Listing
January 2019
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