29,987 results match your criteria Journal of clinical microbiology[Journal]


Development of Molecular Methods for the Rapid Differentiation of Vaccine Strains from Field Isolates.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary

is among the economically most significant mycoplasmas causing production losses in poultry.Seven melt-curve and agarose gel based mismatch amplification mutation assays (MAMA) and one polymerase chain reaction (PCR) are provided in the present study to distinguish the vaccine strains and field isolates based on mutations in , , , , , and genes. A total of 239 samples ( vaccine and type strains, pure cultures and clinical samples) originating from 16 countries and from at least eight avian species were submitted to the presented assays for validation or in blind tests. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01084-18DOI Listing

Development of Dengue-type NS1 ELISA for Rapid Detection and Differentiation of Dengue Serotypes during Early Infection.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Institute of Preventive Medicine, National Defense Medical Center, Taipei City, Taiwan

Dengue fever, caused by infections with the dengue virus (DENV), affects nearly four hundred million people globally every year. Early diagnosis and management can reduce the morbidity and mortality rates of severe forms of dengue disease as well as decrease the risk of wider outbreaks. Although early diagnosis of dengue can be achieved using a number of commercial NS1 detection kits, none of these can differentiate among the four dengue virus serotypes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00221-19DOI Listing
April 2019
1 Read

Comparative Evaluation of Enteric Bacterial Culture and a Molecular Multiplex Syndromic Panel in Children with Acute Gastroenteritis.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Sections of Pediatric Emergency Medicine and Gastroenterology, Alberta Children's Hospital, Alberta Children's Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada

: Although enteric multianalyte syndromic panels are increasingly employed, direct comparisons with traditional methods and the inclusion of host phenotype correlations are limited.: 3089 children with gastroenteritis submitted stool, rectal-swab specimens, and clinical data. Primary outcome was bacterial pathogen detection agreement for shared targets between culture and the Luminex xTAG® Gastrointestinal Pathogen Panel (GPP). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00205-19DOI Listing
April 2019
1 Read

GeneXpert MTB/RIF is superior to BBD Max MDR-TB for diagnosis of TB in a country with low incidence of MDR-TB.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Kuwait TB Control Unit, Shuwaikh, Kuwait.

With 10 million active disease cases, 558000 cases of rifampin-resistant/multidrug-resistant tuberculosis (MDR-TB) and 1.6 million deaths recorded worldwide in 2017, TB is a leading cause of death from a single infectious agent (1).…. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00537-19DOI Listing
April 2019
1 Read

Evaluation of the ELITe InGenius PCR for Detection of .

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA

, Mpn, is the leading cause of bacterial community acquired pneumonia in persons of all ages. Due to the fastidious nature of this bacteria and the necessary specialized growth media, nucleic acid amplification testing is currently the most reliable means for patient diagnostics. Analytical sensitivity, specificity, reproducibility, and clinical performance of the ELITe InGenius automated PCR platform with its MGB Alert Real-Time PCR research use only reagents (ELITechGroup, Inc. Read More

View Article

Download full-text PDF

Source
http://jcm.asm.org/lookup/doi/10.1128/JCM.00287-19
Publisher Site
http://dx.doi.org/10.1128/JCM.00287-19DOI Listing
April 2019
4 Reads

Societal Implications of the Internet of Pathogens.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Department of Laboratory Medicine, University of Washington, Seattle, WA

The growth of pathogen genomics shows no signs of abating. Whole genome sequencing of clinical viral and bacterial isolates continues to grow at near exponential bounds. Reductions in cost driven by new technology have created a seamless environment for generating, sharing, and analyzing pathogen genomes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01914-18DOI Listing

MRSA harboring still elude us in East London.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Microbiology, Royal London Hospital, Barts Health NHS Trust, London, UK.

Reports of methicillin-resistant harboring the gene have increased in the UK since first being described.To our diagnostic multiplex PCR, a primer set was designed and implemented, then the prevalence in our patient population was investigated.Less than 1% of clinical isolates possessed the gene, confirming that remains the dominant genetic determinant of MRSA in East London. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00020-19DOI Listing

Understanding and Addressing CLSI Breakpoint Revisions - A Primer for Clinical Laboratories.

J Clin Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Los Angeles County Department of Public Health, Los Angeles, CA, 90049.

The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints for bacteria that grow aerobically since 2010. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the and , daptomycin breakpoints for spp., and ceftaroline breakpoints for Implementation of the revisions is a challenge for all laboratories, as not all systems have FDA clearance for the revised (current) breakpoints, compounded by the need for laboratories to perform validation studies and to make updates to laboratory information system / electronic medical record builds in the setting of limited information technology infrastructure. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00203-19DOI Listing
April 2019
4 Reads

Diagnostic accuracy of stool Xpert MTB/RIF for the detection of pulmonary tuberculosis in children: a systematic review and meta-analysis.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Epidemiology, Biostatistics, and Occupational Health, McGill University, Montreal, Canada

Invasive collection methods are often required to obtain samples for the microbiologic evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic-acid amplification testing of easier to collect stool samples could be a non-invasive method of diagnosing PTB. We conducted a systematic review and meta-analysis to evaluate the diagnostic accuracy of testing stool with the Xpert MTB/RIF assay ('stool Xpert') for childhood PTB. Read More

View Article

Download full-text PDF

Source
http://jcm.asm.org/lookup/doi/10.1128/JCM.02057-18
Publisher Site
http://dx.doi.org/10.1128/JCM.02057-18DOI Listing
April 2019
2 Reads
3.993 Impact Factor

Xpert MTB/RIF Ultra for the Detection of in Cerebrospinal Fluid.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Medical Microbiology, Makerere University, Kampala, Uganda.

Tuberculous meningitis (TBM) is the most lethal manifestation of tuberculosis and requires rapid diagnosis and initiation of treatment to prevent death and serious neurological disability.…. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00249-19DOI Listing
April 2019
3 Reads

A systematic review and meta-analysis of the diagnostic accuracy of nucleic acid amplification tests in cerebrospinal fluid for tuberculous meningitis.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Division of Infectious Diseases, Department of Medicine, University of Kansas, Kansas City, KS, USA.

Diagnosis of tuberculous meningitis (TBM) is difficult and poses a significant challenge to physicians worldwide. Recently, nucleic acid amplification (NAA) tests have shown promise for diagnosis of TBM, although performance has been variable. We undertook a systematic review and meta-analysis to evaluate the diagnostic accuracy of NAA tests in cerebrospinal fluid (CSF) samples against culture as the reference standard or a combined reference standard (CRS) for TBM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01113-18DOI Listing
April 2019
1 Read

hicap: serotyping of the capsule locus.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Australia.

exclusively colonises the human nasopharynx and can cause a variety of respiratory infections as well as invasive diseases including meningitis and sepsis. A key virulence determinant of is the polysaccharide capsule of which six serotypes are known, each encoded by a distinct variation of the capsule biosynthesis locus (-a to -f). type b (Hib) was historically responsible for the majority of invasive disease and prevalence has been markedly reduced in countries that have implemented vaccination programs targeting this serotype. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00190-19DOI Listing
April 2019
1 Read

Factors that influence confirmation of positivity by molecular methods.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Laboratory Medicine, Royal Infirmary of Edinburgh, NHS Lothian, Edinburgh, UK.

Several (NG) nucleic acid amplification tests (NAATs) exist with high sensitivity. However, specificity of NG NAAT testing may be suboptimal, particularly in extra-genital biospecimens. Consequently, confirmation with a second NAAT is common, although represents a burden on resources. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.02068-18DOI Listing

Phylogenetic analysis of strains in Wales using core genome MLST to analyse whole genome sequencing data.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Swansea University Medical School, Institute of Life Science, Swansea University, Swansea, Wales, UK.

Inability to standardize the bioinformatic data produced by whole genome sequencing (WGS) has been a barrier to its widespread use in tuberculosis phylogenetics. The aim of this study was to carry out a phylogenetic analysis of tuberculosis in Wales, using Ridom Seqsphere software for core genome MLST (cgMLST) analysis of whole genome sequencing data. The phylogenetics of tuberculosis in Wales has not previously been studied. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.02025-18DOI Listing

Pathotyping the Zoonotic Pathogen : Novel Genetic Markers to Differentiate Invasive Disease-Associated Isolates from Non-Disease Associated Isolates from England and Wales.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Veterinary Medicine, University of Cambridge, Cambridge, UK

is one of the most important zoonotic bacterial pathogens of pigs causing significant economic losses to the global swine industry. is also a very successful coloniser of mucosal surfaces and commensal strains can be found in almost all pig populations worldwide, making detection of the species in asymptomatic carrier herds of little practical value in predicting the likelihood of future clinical relevance. The value of future molecular tools for surveillance and preventative health management lies in the detection of strains that genetically have increased potential to cause disease in presently healthy animals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01712-18DOI Listing

Evidence of sharing between healthy companion animals and co-habiting humans.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal

This study aimed to characterize the fecal colonization and sharing of between companion animals and humans living in close contact.Fecal samples were collected from 50 healthy participants (24 humans, 18 dogs and 8 cats) belonging to 18 households. Samples were plated onto MacConkey agar plates (MCK) with and without cefotaxime or meropenem supplementation. Read More

View Article

Download full-text PDF

Source
http://jcm.asm.org/lookup/doi/10.1128/JCM.01537-18
Publisher Site
http://dx.doi.org/10.1128/JCM.01537-18DOI Listing
April 2019
1 Read

The population among Cystic Fibrosis patients in Quebec, Canada: a disease hotspot without known epidemic isolates.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Institute for integrative and systems biology (IBIS), Université Laval CSSS de Chicoutimi Université de Montréal Université de Sherbrooke Institut universitaire de cardiologie et de pneumologie de Québec (IUCPQ) INRS Institut Armand-Frappier McGill University Centre intégré de santé et de services sociaux de l'Abitibi-Témiscamingue, Hôpital de Rouyn-Noranda

The Canadian province of Quebec has among the highest prevalence rates of Cystic Fibrosis (CF) in the world, with an average of 1 in 2,500 newborns (cysticfibrosis.ca, 1), and up to 1 in 902 in the region of Saguenay-Lac-Saint-Jean (2-4).…. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.02019-18DOI Listing
April 2019
3 Reads

Evaluation of the Liat Cdiff assay for the direct detection of toxin genes within 20 minutes.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Leiden University Medical Center, Dept. of Medical Microbiology, Leiden, The Netherlands

Background: is the main causative agent of antibiotic-associated diarrhoea. Prompt diagnosis is required for initiation of timely infection control measures and appropriate adjustment of antibiotic treatment. The cobas® Cdiff assay for use on the cobas® Liat® System enables a diagnostic result in 20 minutes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00416-19DOI Listing

Comparison of Three Nucleic Acid Amplification Tests (NAATs) and Culture for Detection of Group B Streptococcus (GBS) from Enrichment Broth.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Pathology, University of California, San Diego, La Jolla, CA

Colonization of the gastrointestinal and genitourinary tracts of pregnant women with Group B (GBS) can result in vertical transmission to neonates during labor/delivery. GBS infections in neonates can cause severe complications such as sepsis, meningitis, and pneumonia. Accurate detection is critical because administration of intrapartum antibiotics can significantly reduce transmission. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01958-18DOI Listing
April 2019
1 Read

Pigment Visibility on Rectal Swabs Used to Detect Enteropathogens: A Prospective Cohort Study.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Sections of Pediatric Emergency Medicine and Gastroenterology, Department of Pediatrics, Alberta Children's Hospital, Alberta Children's Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.

Data are lacking regarding the impact of visible pigment on rectal swab diagnostic accuracy. We describe the test characteristics of rectal swabs with and without pigment in children with gastroenteritis. Between December 2014 and September 2017, children <18 years with ≥3 episodes of vomiting and/or diarrhea in a 24-hour period and symptoms <7 days were enrolled through two pediatric emergency departments, and from a province-wide nursing telephone advice line in Alberta, Canada. Read More

View Article

Download full-text PDF

Source
http://jcm.asm.org/lookup/doi/10.1128/JCM.00213-19
Publisher Site
http://dx.doi.org/10.1128/JCM.00213-19DOI Listing
April 2019
1 Read

Ultrasensitive Detection of Toxins Reveals Suboptimal Accuracy by Toxin-Gene Cycle-Thresholds for Toxin Predictions.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Leeds Teaching Hospitals NHS Trust and University of Leeds.

The use of nucleic acid amplification tests (NAATs) for the diagnosis of () infection (CDI) leads to overdiagnosis. To improve the clinical specificity of NAATs, there has been a recent interest in using toxin-gene cycle-thresholds (CT) to predict the presence and absence of toxins. Although there is an association between CT values and fecal-toxin concentrations, the predictive accuracy of the former is suboptimal for use in clinical practice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01885-18DOI Listing

Evaluation of the Bio-Rad Geenius HIV 1/2 Assay as Part of a Confirmatory HIV Testing Strategy for Quebec: Comparison with Western Blot and Inno-Lia Assays.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Laboratoire de santé publique du Québec/Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada.

The rapid confirmatory Bio-Rad Geenius HIV 1/2 assay was evaluated as an alternative to the HIV-1 Western Blot (WB) confirmatory assay.A total of 370 retrospective samples collected from 356 patients were tested. Sensitivity of the Geenius assay to detect HIV-1 and HIV-2 infections was 100% and 97% as compared to 86% and 39% using WB assay, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01398-18DOI Listing
April 2019
2 Reads

Culture of Rectal Swab Specimens for Enteric Bacterial Pathogens Decreases Time to Test Result While Preserving Assay Sensitivity Compared to Bulk Fecal Specimens.

J Clin Microbiol 2019 Apr 3. Epub 2019 Apr 3.

Department of Pathology & Immunology, Washington University in St. Louis School of Medicine

Diarrheal illness is a major cause of morbidity and mortality throughout the world, yet the etiologic agent of many cases of gastrointestinal illness remains unspecified, often due to the lack of convenient, timely and sensitive diagnostic testing. Although bulk fecal specimens remain the recommended specimen type for enteric culture, rectal swabs may be an option preferred by clinicians and patients due to convenience and timing of collection. However, the lack of data evaluating the sensitivity of rectal swabs compared to fecal specimens for detection of enteric pathogens precludes this specimen type from being recommended by national guidelines. Read More

View Article

Download full-text PDF

Source
http://jcm.asm.org/lookup/doi/10.1128/JCM.02077-18
Publisher Site
http://dx.doi.org/10.1128/JCM.02077-18DOI Listing
April 2019
4 Reads

Closing the Brief Case: Postinfectious Glomerulonephritis as an Unexpected Sequela of Drinking Raw Milk.

J Clin Microbiol 2019 Apr 28;57(4). Epub 2019 Mar 28.

Departments of Laboratory and Pathology, Cook Children's Medical Center, Fort Worth, Texas, USA

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01790-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440782PMC

The Brief Case: Postinfectious Glomerulonephritis as an Unexpected Sequela of Drinking Raw Milk.

J Clin Microbiol 2019 Apr 28;57(4). Epub 2019 Mar 28.

Departments of Laboratory and Pathology, Cook Children's Medical Center, Fort Worth, Texas, USA

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01767-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440773PMC

Answer to April 2019 Photo Quiz.

J Clin Microbiol 2019 Apr 28;57(4). Epub 2019 Mar 28.

Department of Microbiology, Ng Teng Fong General Hospital, Jurong East, Singapore.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00208-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440784PMC
April 2019
1 Read

Photo Quiz: An Unusual Knee Infection.

J Clin Microbiol 2019 Apr 28;57(4). Epub 2019 Mar 28.

Department of Microbiology, Ng Teng Fong General Hospital, Jurong East, Singapore.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00207-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440793PMC
April 2019
1 Read

The Divergent Meanings of "I" for the Clinical and Laboratory Standards Institute and the European Committee on Antimicrobial Susceptibility Testing: a Micro-Comic Strip.

J Clin Microbiol 2019 Apr 28;57(4). Epub 2019 Mar 28.

Department of Laboratory Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00050-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440775PMC

Molecular typing of strains in Sweden, 1996-2017, and the emergence of a new P1 cytadhesin gene, Variant 2e.

J Clin Microbiol 2019 Mar 27. Epub 2019 Mar 27.

Department of Medical Sciences, Section of Clinical Microbiology and Infectious Diseases, Uppsala University, Uppsala, Sweden.

causes respiratory infections, such as community-acquired pneumonia (CAP), with epidemics recurring every 3-7 years. In 2010-2011, many countries experienced an extraordinary epidemic peak. The cause of these recurring epidemics is not understood, but decreasing herd immunity and shifts in the strains' antigenic properties have been suggested as contributing factors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00049-19DOI Listing
March 2019
3 Reads

Genotyping jirovecii using a New Simplified Nomenclature System Based on the Internal Transcribed Spacer Regions and 5.8S rRNA Gene of the rRNA Operon.

J Clin Microbiol 2019 Mar 27. Epub 2019 Mar 27.

Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang, P. R. China

Genotyping based on the internal transcribed spacers I and II (ITS1 and ITS2) of the rRNA operon has played an important role in understanding the transmission and epidemiology of , one of the major opportunistic pathogens in AIDS and other immunocompromised individuals. The widespread use of this typing system has resulted in several problems including inconsistent genotype nomenclatures, difficult data transferability, and complicated interpretation of length variation in multiple homopolymeric tracts. The aim of this study was to establish a new, simplified genotyping nomenclature system based on ITS1 and ITS2 for We first analyzed the complete ITS1, 5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.02012-18DOI Listing

Beta-D-glucan for Diagnosing Pneumocystis Pneumonia: a direct comparison between the Wako β-glucan assay and the Fungitell assay.

J Clin Microbiol 2019 Mar 27. Epub 2019 Mar 27.

Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.

Background: Measuring serum beta-D-glucan (BDG) is a useful tool for supporting a qPCR-based diagnosis of suspected Pneumocystis pneumonia (PCP) on broncho-alveolar lavage (BAL) fluid. Since the 2000's, the Fungitell assay was the only BDG assay which was FDA-cleared and CE-marked. However, the Wako β-glucan test was recently also CE-marked and commercialized. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00322-19DOI Listing
March 2019
1 Read

Triazole Antifungal Susceptibility Patterns among Aspergillus species in Québec, Canada.

J Clin Microbiol 2019 Mar 27. Epub 2019 Mar 27.

Division of Infectious Diseases, Department of Medicine, McGill University, Montreal, Canada.

Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised patients, with over 500 patients developing IA in Canada each year.…. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00404-19DOI Listing
March 2019
1 Read

Methodology for whole genome sequencing of MRSA in a routine hospital microbiology laboratory.

J Clin Microbiol 2019 Mar 20. Epub 2019 Mar 20.

Department of Medicine, University of Cambridge, Box 157 Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0QQ, UK.

There is growing evidence for the value of bacterial whole genome sequencing in hospital outbreak investigation. Our aim was to develop methods that support efficient and accurate low throughput clinical sequencing of methicillin-resistant (MRSA). Using a test panel of 25 MRSA isolates associated previously with outbreak investigations, we devised modifications to library preparation that reduced processing time by 1 hour. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00180-19DOI Listing
March 2019
1 Read

Comparing Patient Risk Factors, Sequence Type, and Resistance Loci Identification Approaches for Predicting Antibiotic Resistance in Bloodstream Infections.

J Clin Microbiol 2019 Mar 20. Epub 2019 Mar 20.

University of Toronto, Division of Infectious Diseases, Toronto, Canada.

Rapid diagnostic tests of antibiotic resistance are increasingly being developed that identify the presence or absence of antibiotic resistance genes/loci. However, these approaches usually neglect other sources of predictive information, which could be identified in shorter time periods, including patient epidemiologic risk factors for antibiotic resistance and markers of lineage. Using a dataset of 414 isolated from separate episodes of bacteremia at a single academic institution in Toronto, Canada between 2010-2015, we compared the potential predictive ability of three approaches (epidemiologic, pathogen sequence type, resistance gene identification) for classifying phenotypic antibiotic resistance to three antibiotics representing classes of broad spectrum antimicrobial therapy (ceftriaxone - 3 generation cephalosporins, ciprofloxacin - fluoroquinolones, and gentamicin - aminoglycosides). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01780-18DOI Listing

Summary and Trends of the Russian Gonococcal Antimicrobial Surveillance Programme, 2005-2016.

J Clin Microbiol 2019 Mar 20. Epub 2019 Mar 20.

State Research Center of Dermatovenerology and Cosmetology, Moscow, Russia

The Russian Gonococcal Antimicrobial Surveillance Programme (RU-GASP) was established in 2004 and operated continuously during the years 2005-2016. The aims of this study were to summarize the RU-GASP results over this 12-year period and evaluate the trends in antimicrobial resistance in Russia. In total, 5,038 verified isolates from 40 participating regions were tested for susceptibility to six antimicrobials via an agar dilution method. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.02024-18DOI Listing
March 2019
1 Read

isolates of the Southern Asian and South African lineages exhibit different phenotypic and antifungal susceptibility profiles .

J Clin Microbiol 2019 Mar 13. Epub 2019 Mar 13.

UK National Mycology Reference Laboratory (MRL), Public Health England South-West, Bristol, United Kingdom.

is a serious nosocomial health risk, with widespread outbreaks in hospitals worldwide. Sequence analyses of outbreak isolates revealed that has simultaneously emerged as four distinct continentally restricted clonal lineages. We previously reported multiple independent introductions of isolates from at least three of these lineages (S. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.02055-18DOI Listing

To Lump or To Split: Does Strain Lineage for Matter?

Authors:
Scott R Curry

J Clin Microbiol 2019 Mar 13. Epub 2019 Mar 13.

Division of Infectious Diseases, Medical University of South Carolina, Charleston, SC, USA

Since 2001, numerous descriptive ecological studies of infections (CDI) have identified a single lineage (BI/NAP1/027) as associated with epidemics of CDI, increased severity of CDI, and increased likelihood of incident CDI to become recurrent. Establishing causality between the clinical severity and outcomes for CDI and the lineages of the infecting strains, however, has proved elusive, with many conflicting results in previous observational studies. In this issue of the , Garneau and Abou Chakra et al. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00196-19DOI Listing

EDTA-Modified Carbapenem Inactivation Method (eCIM): a Phenotypic Method for Detecting Metallo-β-Lactamase-Producing .

J Clin Microbiol 2019 Mar 13. Epub 2019 Mar 13.

Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY, USA

The increase in the prevalence and impact of infections caused by carbapenemase-producing is a global health concern. Therefore, rapid and accurate methods to detect these organisms in any clinical microbiology laboratory, including those in resource limited settings, is essential to prevent and contain their spread. It is also important to differentiate between serine- and metal-dependent carbapenemases elaborated by carbapenemase-producing isolates for epidemiologic, infection control and prevention, and therapeutic purposes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01757-18DOI Listing

Prognostic indicators of recurrence of bacterial vaginosis.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Division of Infectious Diseases, Department of Microbiology, Immunology & Biochemistry, and Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI.

: Following all forms of therapy for bacterial vaginosis (BV), recurrence rates are extremely high. Many diagnostic tests are available that differentiate bacterial vaginosis from other types of vaginal disorders, but none predict recurrence after treatment, nor are any vetted for monitoring ongoing responses to treatment. Our goal is to determine which tests, and at what optimal times, have prognostic value in predicting recurrence. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00227-19DOI Listing
March 2019
2 Reads

Reduced incubation time of the modified carbapenem inactivation test and performance of carbapenem inactivation in a set of carbapenemase producing with a high proportion of IMP isolates.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Accurate and timely identification of carbapenemase producing (CPEs) is critical for microbiology laboratories in order to facilitate infection prevention, reduce the spread of multi-resistant gram-negative bacilli, and reduce delays to effective antibiotic therapy. We undertook a study to compare the carbapenem inactivation method (CIM) against the modified carbapenem inactivation method (mCIM) on a broad range of CPE isolates from Australia including a high proportion of IMP isolates. Furthermore we evaluated the performance of the mCIM with a reduced incubation time using automated incubation and digital plate reading in order to better facilitate quick confirmation of carbapenemases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01852-18DOI Listing

Biographical Feature: Dr. Stephen G. Jenkins, PhD, D(ABMM), F(AAM).

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Editor-in-Chief, Journal of Clinical Microbiology,Associate Professor of Pathology, Harvard Medical School, Medical Director, Infectious Disease Diagnostic Laboratory, Boston Children's Hospital

Dr. Stephen G. Jenkins, D(ABMM), is the recipient of the 2019 ASM Award for Research and Leadership in Clinical Microbiology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00299-19DOI Listing
March 2019
2 Reads

A Novel Phenotypic Method to Screen for Plasmid-Mediated Colistin Resistance Among .

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Dept. of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD

Plasmid-mediated colistin resistance (PMCR), a consequence of the genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution test (CBDE) to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of ethylenediaminetetraacetic acid (EDTA). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00040-19DOI Listing
March 2019
1 Read

Evaluation of Aspergillus-specific Lateral-Flow Device Test using Serum and Bronchoalveolar Lavage Fluid for Diagnosis of Chronic Pulmonary Aspergillosis.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Department of Infectious Diseases, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

-specific lateral-flow device test (AspLFD) is a newly developed point-of-care diagnostic method for invasive pulmonary aspergillosis. However, evidence of the diagnostic performance of the AspLFD for chronic pulmonary aspergillosis (CPA) is limited. Therefore, we conducted a retrospective study to investigate this in comparison with the galactomannan (GM), β-D-glucan (BDG). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00095-19DOI Listing
March 2019
1 Read

Identification of using the VITEK mass spectrometer.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Pathology Queensland, Townsville Hospital, Townsville, Australia.

is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteraemia, with a mortality rate of 9 - 70%. Therefore, early identification of this organism may aid in directing appropriate management. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00081-19DOI Listing
March 2019
1 Read

Impact of early antiretroviral therapy on detection of cell-associated HIV-1 nucleic acid in blood by the Roche COBAS TaqMan Test.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD USA.

The Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 test v2.0 (CAP/CTM) was used to quantify cell-associated HIV-1 nucleic acid (CAH) in peripheral blood mononuclear cells (PBMC) from well-characterized clinical specimens from HIV-1 infected individuals on antiretroviral therapy (ART). Chronically infected individuals on ART with no detectable plasma HIV-1 RNA demonstrated average CAH burdens of 3. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01922-18DOI Listing
March 2019
3 Reads
3.993 Impact Factor

Comparable Serum and Plasma 1,3-β-D-Glucan Values Using the Wako β-Glucan Test Assay in Patients with Probable or Proven Fungal Diseases.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Fondazione Policlinico Universitario A. Gemelli IRCCS, Dipartimento di Scienze Gastroenterologiche, Endocrino-Metaboliche e Nefro-Urologiche, Rome, Italy.

Here, we report comparable results of the Wako β-Glucan test (GT; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), recently launched in Europe for testing the invasive fungal disease (IFD) biomarker 1,3-β-D-glucan (BDG) (1-3), in matched serum and plasma samples from patients with IFD.…. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00149-19DOI Listing

Development and multicentric validation of a lateral flow immunoassay for the rapid detection of MCR-1-producing .

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Service de Pharmacologie et Immuno-analyse (SPI), JOLIOT, CEA, INRA, Université Paris-Saclay, F-91191, Gif sur Yvette, FRANCE.

Colistin has become a last resort antibiotic for the treatment of highly drug-resistant Gram negatives. Moreover, it has been widely used in the livestock sector. As a consequence, colistin resistance is emerging worldwide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01454-18DOI Listing
March 2019
15 Reads

Multiplex detection of three select agents directly from blood using the GeneXpert system.

J Clin Microbiol 2019 Mar 6. Epub 2019 Mar 6.

Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, NJ

and are tier1 select agents with the potential to rapidly cause severe disease. Rapid detection of these bacteria from patient samples at the point of care could contribute to improved clinical outcomes in the event of a bioterrorism attack. A multiplex nested PCR assay for detection of , and directly from patient blood samples was developed using the GeneXpert system. Read More

View Article

Download full-text PDF

Source
http://jcm.asm.org/lookup/doi/10.1128/JCM.00036-19
Publisher Site
http://dx.doi.org/10.1128/JCM.00036-19DOI Listing
March 2019
7 Reads

Closing the Brief Case: Incidental Finding of Cystic Echinococcosis during Evaluation for Hepatitis.

J Clin Microbiol 2019 Mar 27;57(3). Epub 2019 Feb 27.

Department of Pathology, Saint Louis University School of Medicine, St. Louis, Missouri, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.01571-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425165PMC
March 2019
1 Read