3,394 results match your criteria Journal of chromatographic science[Journal]


Modeling Retention Behavior on Analysis of Hallucinogenic Mushrooms Using Hydrophilic Interaction Liquid Chromatography.

J Chromatogr Sci 2018 Dec 7. Epub 2018 Dec 7.

Department of Drug and Arson Investigation, Hungarian Institute for Forensic Sciences, Mosonyi Street 9, Budapest, Hungary.

The goal of this work was to investigate and compare the selectivity of three different hydrophilic interaction liquid chromatography (HILIC) charge modulated amide columns, iHILIC®-Fusion, iHILIC®-Fusion(+) and iHILIC®-Fusion(P), for analysis of compounds in hallucinogen mushrooms. An extract of a truffle-like fungus containing psilocin, psilocybin and baeocystin was chosen as test material. Three different modeling methods were applied to describe the retention times of constituents in isocratic separation mode as a function of mobile phase composition, pH and temperature. Read More

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http://dx.doi.org/10.1093/chromsci/bmy104DOI Listing
December 2018

A High Throughput Three-step Ultra-performance Liquid Chromatography Tandem Mass Spectrometry Method to Study Metabolites of Atractylenolide-III.

J Chromatogr Sci 2018 Nov 29. Epub 2018 Nov 29.

Department of Natural Medicine Chemistry, Institute of Pharmacy, Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Avenue, Nanchang, PR China.

Atractylodes macrocephala Koidz (AMK) is a traditional Chinese medicine widely used in the treatment of various diseases, especially spleen deficiency. As the principle active constituents of AMK, however, the metabolites of Atractylenolide-III (A-lactone-III) have not been identified in rats yet. In this study, a three-step high throughput method based on UHPLC-Q-TOF-MS-MS was developed to profile and characterize the metabolites of A-lactone-III in rat feces, urine and plasma. Read More

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http://dx.doi.org/10.1093/chromsci/bmy098DOI Listing
November 2018

Simultaneous Quantification of Benznidazole and Posaconazole by HPLC-DAD Using QbD Approach.

J Chromatogr Sci 2018 Nov 29. Epub 2018 Nov 29.

Department of Pharmaceutical Sciences, Federal University of Pernambuco, PE, Brazil.

Objective: To develop an analytical method to simultaneous quantification of benznidazole (BNZ) and posaconazole (POS) by high-performance liquid chromatography with diode-array detection (HPLC-DAD) using design of experiments.

Methods: Percentages of organic phase, buffer pH and flow rates of mobile phase were selected as independent variables by full factorial design (33), totaling 27 experiments. Significant factors were evaluated using factorial analysis of variance with 95% confidence level. Read More

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http://dx.doi.org/10.1093/chromsci/bmy097DOI Listing
November 2018

Development and Validation of LC-MS-MS Assay for the Determination of the Emerging Alkylating Agent Laromustine and Its Active Metabolite in Human Plasma.

J Chromatogr Sci 2018 Nov 3. Epub 2018 Nov 3.

Metastagen, Inc., Wilmington, DE, USA.

The objective of this study was to validate a method for the determination of laromustine (VNP40101M) and short-lived its active metabolite (VNP4090CE) that has a half-life in human blood of <90 s in human plasma by liquid chromatography (LC) with tandem mass spectrometric (MS/MS) detection. We overcome the stability dilemma by acidified the human plasma with citric acid. Laromustine "breaks" down on the source of mass spectrometry to give m/z 249 which is the same m/z for VNP4090CE. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy100DOI Listing
November 2018
4 Reads

HPTLC method for Simultaneous Determination of Norfloxacin and Tinidazole in presence of Tinidazole Impurity.

J Chromatogr Sci 2018 Nov 3. Epub 2018 Nov 3.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., Beni-Suef, Egypt.

Sensitive, selective and accurate high-performance thin layer chromatographic HPTLC method for quantitative determination of Norfloxacin (NF), Tinidazole (TZ) and 2-Methyl-5-nitroimidazole (MNZ) as potential impurity of Tinidazole is developed and validated in the presented work. Calibration curves were linear over the concentration ranges of 0.4-2. Read More

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http://dx.doi.org/10.1093/chromsci/bmy085DOI Listing
November 2018
3 Reads

Separation and Characterization of Unknown Impurities and Isomers in Cefminox Sodium and Study of the Forming Mechanisms of Impurities by Liquid Chromatography Coupled with Ion Trap/Time-Of-Flight Mass Spectrometry.

J Chromatogr Sci 2018 Nov 3. Epub 2018 Nov 3.

Zhejiang University of Technology, College of Pharmacy, Hangzhou, China.

Thirteen unknown impurities and isomers in cefminox sodium were separated and characterized by liquid chromatography coupled with high-resolution ion trap/time-of-flight mass spectrometry (LC-IT-TOF-MS) with the positive mode of electrospray ionization (ESI) method. New HPLC-gradient elution method was developed for the detection of impurities in cefminox sodium. And the ESI ion trap multiple-stage tandem mass spectrometry had been applied successfully to the direct investigation of impurities and isomers in cefminox sodium. Read More

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http://dx.doi.org/10.1093/chromsci/bmy101DOI Listing
November 2018
5 Reads

Stability-Indicating RP-UPLC Method for Simultaneous Determination of Azilsartan Medoxomil and Chlorthalidone in Tablets in the Presence of Its Degradation Products.

J Chromatogr Sci 2018 Nov 3. Epub 2018 Nov 3.

Research and Development Department, Eva Pharma for Pharmaceutical and Medical Appliances, Giza, Egypt.

Azilsartan Medoxomil (AZL) angiotensin II receptor blocker and chlorthalidone (CLT) were determined by ultraperformance liquid chromatography (UPLC) method in their combined dosage form, they were both subjected to forced degradation studies under extensive stress conditions. The method is a stability indicating by resolving the investigated drugs from their degradation products. Moreover, the degradation products for both drugs obtained from forced degradation were subjected to LC-MS for structure elucidation. Read More

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http://dx.doi.org/10.1093/chromsci/bmy102DOI Listing
November 2018
10 Reads

Application of Dopant-Assisted Atmospheric Pressure Photoionisation HPLC-MS Method for the Sensitive Determination of Polycyclic Aromatic Hydrocarbons in Dark Chocolate.

J Chromatogr Sci 2018 Nov 3. Epub 2018 Nov 3.

Institute of Food Safety, Animal Health and Environment "BIOR", Lejupes Street 3, Riga, Latvia.

Multiple food research studies have shown that the polycyclic aromatic hydrocarbon (PAHs) are frequently found in processed cocoa products and chocolate. In a present study a method based on dopant-assisted atmospheric pressure photoionisation (DA-APPI) combined with a liquid chromatography/high-resolution mass spectrometer (HPLC-HRMS) for high-sensitivity analysis of four EU marker PAHs in dark chocolate samples was developed and fully validated according to the performance criteria set in EU guidelines. PAHs detection was achieved by HRMS in positive electrospray ionization mode with toluene used as a dopant to enhance the ionization efficiency of non-polar PAHs. Read More

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http://dx.doi.org/10.1093/chromsci/bmy103DOI Listing
November 2018
1 Read

A High-Performance Liquid Chromatography Method for Determination of Genotoxic Impurity Hydroxylamine in Drug Substances.

J Chromatogr Sci 2018 Oct 29. Epub 2018 Oct 29.

Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, India.

Hydroxylamine (NH2OH) is widely used in pharmaceutical intermediates and final drug substances synthesis. Since hydroxylamine is a well-known genotoxic impurity compound that needs to be controlled down to ppm level in pharmaceutical compounds. It is very difficult to detect using conventional analytical techniques due to its physical-chemical properties like the lack of chromophore, low molecular weight, the absence of carbon atom and high polarity. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy082DOI Listing
October 2018
9 Reads

HPTLC-Densitometric Method for Determination of Ascorbic Acid, Paracetamol and Guaifenesin in Presence of Their Toxic Impurities.

J Chromatogr Sci 2018 Oct 26. Epub 2018 Oct 26.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., Beni-Suef, Egypt.

Ascorbic acid (ASC), paracetamol (PAR) and guaifenesin (GUF) are co-formulated together as analgesic and expectorant effervescent powder. This work describes a selective stability indicating HPTLC-densitometric method which is the first developed stability indicating one for chromatographic separation of the three studied drugs in presence of paracetamol toxic impurity [4-aminophenol (4-AP)] and guaifenesin related substance [impurity, (guaiacol) (GUC)]. The chromatographic separation was carried out on HPTLC 60F254 plates using chloroform-acetone-trifluoroacetic acid (6. Read More

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http://dx.doi.org/10.1093/chromsci/bmy096DOI Listing
October 2018

Green Tailoring With Water of Choline Chloride Deep Eutectic Solvents for the Extraction of Polyphenols From Palm Samples.

J Chromatogr Sci 2018 Oct 26. Epub 2018 Oct 26.

College of Pharmaceutical Science, Hebei University, 180 Wusi East Road, Baoding, China.

To address the problem of high viscosity of deep eutectic solvents (DESs), the viscosities of a series of choline chloride-based DESs and DESs-H2O were determined, and their extraction efficiencies were obtained for the extraction of polyphenols in palm samples. Tailoring the DESs by the addition of water successfully adjusted their viscosity from thousand to single-digit mPa s and aided the extraction of polyphenols from samples, by changing the hydrogen-bonding interactions of DESs. Among the compounds investigated, choline chloride-oxalic acid-water of DESs-H2O with various molar ratios with 1:0. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy099DOI Listing
October 2018
6 Reads

Non-Anthocyanin Phenolic Compounds in Açaí (Euterpe oleracea Mart.) Juice by Ultrahigh-Performance Liquid Chromatography-Diode Array Detector (UHPLC-DAD): A Multivariate Optimization.

J Chromatogr Sci 2018 Oct 26. Epub 2018 Oct 26.

Department of Food Science. Faculty of Food Engineering, University of Campinas (UNICAMP), Monteiro Lobato Street, Campinas, SP, Brazil.

Currently available high-performance liquid chromatographic (HPLC) methods for the analysis of non-anthocyanin compounds in açaí are time-consuming or employ complex and costly detection systems. Therefore, we hypothesized that by using ultrahigh-performance liquid chromatography interfaced with a diode array detector (UHPLC-DAD) in conjunction with multivariate optimization tools (response surface methodology, Derringer and Suich desirability) would give a simple, fast, effective and reliable method to determine eight phenolic acids and four flavonoids in açaí juice. Three independent variables were assessed: initial acetonitrile (ACN) concentration in the gradient elution, final ACN concentration at the end of the gradient and the linear time of the gradient elution. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/chromsci/bmy095DOI Listing
October 2018
2 Reads

Analysis of Triacylglycerols in Castor Oil Through Liquid Chromatography-Mass Spectrometry Based on Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry and Gas Chromatography-Mass Spectrometry.

J Chromatogr Sci 2018 Oct 6. Epub 2018 Oct 6.

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China.

Castor oil is a traditional Chinese medicine containing a chemically complex mixture of triacylglycerols (TAGs). Herein, the TAGs in castor oil were analyzed in detail. First, the fatty acid composition of castor oil was examined via methylated derivatisation followed by gas chromatography-mass spectrometry (GC-MS). Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy088DOI Listing
October 2018
1 Read

A Validated GC-MS Method for the Determination of Genotoxic Impurities in Divalproex Sodium Drug Substance.

J Chromatogr Sci 2018 Oct 4. Epub 2018 Oct 4.

Aurobindo Pharma Limited Research Centre-II, Survey No: 71&72, Indrakaran Village, Kandi Mandal, Sangareddy, Telangana, India.

A specific GC-MS method has been developed, optimized and validated for the determination of five genotoxic impurities namely Methyl bromide (Me.-Br), Ethyl bromide (Et.-Br), Isopropyl bromide (Ipr. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy089DOI Listing
October 2018
2 Reads

Sensitivity Enhancement for Direct Injection Capillary Electrophoresis to Determine Morphine in Human Serum via In-capillary Derivatization.

J Chromatogr Sci 2018 Oct 1. Epub 2018 Oct 1.

Laboratory for Single Cell Mass Spectrometry, RIKEN Quantitative Biology Center, 6-2-3, Furuedai, Suita, Osaka, Japan.

Rapid and simple micellar electrokinetic chromatography (MEKC) with in-capillary derivatization and fluorescence detection has been developed to determine morphine in human serum. The sample was introduced into a background electrolyte (BGE) containing potassium ferricyanide, whereas morphine was oxidized into highly fluorescent product, pseudomorphine. Different parameters for derivatization and subsequent separation were systematically investigated for the analysis of morphine in serum. Read More

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http://dx.doi.org/10.1093/chromsci/bmy092DOI Listing
October 2018
1 Read
1.360 Impact Factor

Simultaneous Determination of Thalidomide and Dexamethasone in Rat Plasma by Validated HPLC and HPTLC With Pharmacokinetic Study.

J Chromatogr Sci 2018 Oct 1. Epub 2018 Oct 1.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St, Cairo, Egypt.

Two validated chromatographic methods have been developed for the simultaneous determination of thalidomide (THD) and dexamethasone (DEX) in rat plasma using paracetamol (PAR) as an internal standard (IS). Chromatographic analysis was achieved firstly by HPLC method on C18 column (150 × 4.6 mm2 i. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy094DOI Listing
October 2018
9 Reads

Matrix Solid-Phase Dispersion Combined with HPLC-DAD for Simultaneous Determination of Nine Lignans in Saururus chinensis.

J Chromatogr Sci 2018 Oct 1. Epub 2018 Oct 1.

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.

A simple and rapid method, based on matrix solid-phase dispersion (MSPD) and high-performance liquid chromatography (HPLC) was developed for simultaneous determination of nine lignans, including (-)-(7R,8R)-machilin D (Wang, C., Wang, P., Chen, X. Read More

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http://dx.doi.org/10.1093/chromsci/bmy090DOI Listing
October 2018
3 Reads

The Effect of Monolith Properties on the Digestion Performance of Monolith-Based Immobilized Enzyme Microreactor.

J Chromatogr Sci 2018 Oct 1. Epub 2018 Oct 1.

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Normal University, Cihu Road No. 11, Huangshi, China.

In this work, two kinds of monolith-based immobilized enzyme microreactors (IMER) were compared in terms of monolith properties and digestion performance. Two monoliths were prepared involving poly (tetraethoxysiLane-co-3-aminopropyltriethoxysilane) (poly (TEOS-co-APTES)) monolith and poly (N-acryloxysuccinimide-co-acrylamide-co-ethylene dimethacrylate) (poly (NAS-co-AA-co-EDMA)) monolith for covalent trypsin immobilization. A higher chromatographic permeability constant 66. Read More

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http://dx.doi.org/10.1093/chromsci/bmy091DOI Listing
October 2018
7 Reads

Simultaneous Determination of Ten Bioactive Components in Raw and Processed Radix Dipsaci by UPLC-Q-TOF-MS.

J Chromatogr Sci 2018 Sep 21. Epub 2018 Sep 21.

Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.

Radix Dipsaci is employed for treating bone fractures in traditional Chinese medicine. In the current, a quantitative approach was established for determination of ten bioactive components (loganic acid, loganin, 3,5-diCQA, 4-CQA, 3,4-diCQA, sweroside, dipsacussaponin B, dipsacoside A, asperosaponin V and asperosaponin VI) in raw and rice wine-sautéed Radix Dipsaci by using UPLC-Q-TOF-MS based approach. The sample handling procedure was optimized. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy093DOI Listing
September 2018
2 Reads

Quantitative Analysis of Polycyclic Aromatic Hydrocarbons at Part Per Billion Levels in Fish Oil by Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS).

J Chromatogr Sci 2018 Sep 12. Epub 2018 Sep 12.

Department of Chemistry and Biochemistry, Center for Academic Industry Partnership, Seton Hall University, 400 South Orange Avenue, South Orange, NJ, USA.

Headspace solid-phase microextraction (HS-SPME) was used in combination with gas chromatography-mass spectrometry (GC-MS) for the analysis of polycyclic aromatic hydrocarbons (PAH) at part per billion levels in fish oil samples collected from menhaden fish. The method was initially developed using fish oil from capsules spiked with a standard PAH mixture. The final HS-SPME-GC-MS method presented a linear range from 3 to 1,500 ng/g, with precision for most analytes <10% relative standard deviation. Read More

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http://dx.doi.org/10.1093/chromsci/bmy086DOI Listing
September 2018
1 Read

Dispersive Liquid-Liquid Microextraction of Famotidine and Nizatidine from Water Samples.

J Chromatogr Sci 2018 Aug 31. Epub 2018 Aug 31.

Institute of Chemistry, Biological-Chemical Department, University of Bialystok, 15-245 Bialystok, Poland.

A new extraction method was developed for isolation of the antihistaminic drugs (histamine H2 receptor antagonists) famotidine (FMT) and nizatidine (NZT) from aqueous samples. A low solvent consumption method using dispersive liquid-liquid microextraction (DLLME) was applied for this purpose. The parameters of the isolation, namely, volume of extraction and disperser solvents, pH of sample and shaking time were optimized. Read More

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http://dx.doi.org/10.1093/chromsci/bmy087DOI Listing
August 2018
2 Reads

Atom-Type-Based AI Topological Indices for Artificial Neural Network Modeling of Retention Indices of Monomethylalkanes.

J Chromatogr Sci 2018 Aug 28. Epub 2018 Aug 28.

Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, I.R. Iran.

In this work, a combination of Xu and atom-type-based AI topological indices (TIs) were employed for quantitative structure-retention relationship (QSRR) study of monomethylalkanes (MMAs). A total of 196 temperature-programmed gas chromatographic retention indices corresponding to all C4-C30 MMAs on OV-1 stationary phase have been used in QSRR modeling. Results of the study showed that an artificial neural network (ANN) with 4-9-1 topology and Levenberg-Marquardt training algorithm can predict the retention indices with high degree of accuracy. Read More

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http://dx.doi.org/10.1093/chromsci/bmy081DOI Listing
August 2018
1 Read

A Stability-Indicating UPLC Method for the Determination of Potential Impurities and Its Mass by a New QDa Mass Detector in Daclatasvir Drug Used to Treat Hepatitis C Infection.

J Chromatogr Sci 2018 Aug 28. Epub 2018 Aug 28.

Analytical R&D, Hetero Labs Ltd, Hyderabad, Telangana, India.

A stability-indicating ultraperformance liquid chromatographic method has been developed for the quantitative determination of degradation products and process-related impurities of daclatasvir in a pharmaceutical dosage form. Chromatographic separation was achieved on a polar Waters ACQUITY BEH phenyl 100 × 2.1 mm, 1. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy079DOI Listing
August 2018
16 Reads

An Alternative HPLC with Ultraviolet Detection for Determination of Piperaquine in Plasma.

J Chromatogr Sci 2018 Aug 31. Epub 2018 Aug 31.

Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Graduate Studies, Thammasat University (Rangsit Campus), Paholyothin Road, Pathumthani 12121, Thailand.

A simple, sensitive, selective and reproducible method based on liquid chromatography was developed for the determination of piperaquine in human plasma samples. Sample preparation involved a single step liquid-liquid extraction by organic solvents (hexane: tert-butyl methylether at the ratio of 1:1, v:v). Piperaquine was separated from the internal standard mefloquine on a reversed-phase C18 column, with the mobile phase consisting of a mixture of acetonitrile and 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy077DOI Listing
August 2018
2 Reads

Development of a Simple, Highly Selective RP-LC Method for the Quantification of Diastereomers and Other Related Substances of Ezetimibe Using Multivariate Analysis.

J Chromatogr Sci 2018 Aug 25. Epub 2018 Aug 25.

IPDO, Dr. Reddy's Laboratories Ltd, Hyderabad, India.

A simple reverse phase method for the selective quantification of ezetimibe (EZM), its diastereomers and other related substances was developed. The method demonstrated an excellent separation between each of the 14 impurities (including diastereomers, specified impurities and degradation products) and EZM within a runtime of 45 min. The developed method was evaluated against the reported USP method, other literature methods found that none of them was able to separate/show the absence of all the diastereomers and degradation products. Read More

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http://dx.doi.org/10.1093/chromsci/bmy084DOI Listing
August 2018
5 Reads

Comparison of Chiral Recognition of Binaphthyl Derivatives with l-Undecyl-Leucine Surfactants in the Presence of Arginine and Sodium Counterions.

J Chromatogr Sci 2018 Aug 28. Epub 2018 Aug 28.

Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Howard University, 520 W Street NW, Washington, DC, USA.

In this study the chiral selectivity of l-undecyl-leucine (und-leu) for binapthyl derivatives was examined with the use of arginine and sodium counterions at pH's ranging from 7 to 11. The objective of this project was to investigate whether a cationic amino acid, such as arginine would achieve enhanced chiral selectivity when utilized as the counterion in the place of sodium in micellar electrokinetic chromatography. The data indicate that und-leu has significantly improved chiral selectivity toward 1,1'-binaphthyl-2,2'-diyl hydrogenphosphate (BNP) enantiomers in the presence of arginine counterions in comparison to sodium and that, at least in the case of this study, the enantiomeric form of the arginine did not appear to play a role in the chiral selectivity. Read More

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http://dx.doi.org/10.1093/chromsci/bmy080DOI Listing
August 2018
1 Read

Determination of Zoledronic Acid and Its Related Substances by High Performance Liquid Chromatography with Evaporative Light Scattering Detection.

J Chromatogr Sci 2018 Aug 25. Epub 2018 Aug 25.

Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Beyazit, Istanbul, Turkey.

A method has been developed and validated for analysis of zoledronic acid (ZOL) and its related substances by ion-pair reversed-phase high performance liquid chromatography with evaporative light scattering detection (ELSD). Chromatographic separation was achieved with gradient elution by using a C18 column, mobile phase containing 12 mM ammonium acetate buffer and 35 mM n-pentylamine, whose pH value is 7.0, and 5% acetonitrile. Read More

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http://dx.doi.org/10.1093/chromsci/bmy078DOI Listing
August 2018
1 Read

Simultaneous Determination of Dexpanthenol, Lidocaine Hydrochloride, Mepyramine Maleate and their Related Substances by a RP-HPLC Method in Topical Dosage Forms.

J Chromatogr Sci 2018 Nov;56(10):903-911

Hacettepe University Pharmaceutical Technology Department, Sihhiye, Ankara, Turkey.

The pharmaceutical combination of dexpanthenol (DPA), lidocaine hydrochloride (LIH) and mepyramine maleate (MAM) is used for their anti-allergic, anti-inflammatory, anti-pruritic, anesthetic and antiseptic properties. The present study was aimed to develop and validate a new, first and rapid high performance liquid chromatographic method for simultaneous determination of DPA, LIH and MAM in the presence of their stress-induced degradation products in pharmaceutical gel/fluigel formulations. The chromatographic separation was performed on an Inertsil ODS-3 V, 250 × 4. Read More

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http://dx.doi.org/10.1093/chromsci/bmy067DOI Listing
November 2018
7 Reads

Covalent Immobilization of Protein A on Chitosan and Aldehyde Double-Branched Chitosan as Biocompatible Carriers for Immunoglobulin G (Igg) Purification.

J Chromatogr Sci 2018 Nov;56(10):933-940

Systems Biotechnology Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajohesh Blvd, 17th Km of Tehran-Karaj Highway, Tehran, Iran.

High-affinity ligands, such as protein A, can be used to develop biocompatible matrices for antibody purification. In this paper, two methods were used for immobilization of protein A on the chitosan. In the first approach, amino groups of chitosan beads were functionalized with tris(2-aminoethyl)amine to produce amine double-branched moieties, which were subsequently activated with glutaraldehyde. Read More

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http://dx.doi.org/10.1093/chromsci/bmy070DOI Listing
November 2018
2 Reads
1.363 Impact Factor

Analysis of Perfluorinated Compounds in Environmental Water Using Decyl-perfluorinated Magnetic Mesoporous Microspheres as Magnetic Solid-Phase Extraction Materials and Microwave-Assisted Derivatization Followed by Gas Chromatography-mass Spectrometry.

J Chromatogr Sci 2018 Nov;56(10):955-961

Key Laboratory of Applied Organic Chemistry, Higher Institutions of Jiangxi Province, School of Chemstry and Envicronmental Science, Shangrao Normal University, Shangrao, China.

In this work, a new method was developed for perfluorinated compounds (PFCs) analysis in water samples based on decyl-perfluorinated magnetic mesoporous nanocomposites microspheres-assisted extraction and microwave-assisted derivatization followed by gas chromatography-mass spectrometry analysis. The decyl-perfluorinated magnetic mesoporous nanocomposites have several advantages such as fast separation ability, good dispersibility in water sample and high selective preconcentration of PFCs. Various parameters, including eluting solvent and volume, the amounts of absorbents, extraction time and elution time, the microwave-assisted derivatization conditions were optimized. Read More

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http://dx.doi.org/10.1093/chromsci/bmy073DOI Listing
November 2018
1 Read

Separation and Determination of Selected Polyphenols from Medicinal Plants.

J Chromatogr Sci 2018 Jul 31. Epub 2018 Jul 31.

Department of Inorganic, Analytical Chemistry and Electrochemistry, Silesian University of Technology, 7M. Strzody Str., Gliwice, Poland.

A study of the simultaneous separation and determination of selected polyphenols (rutin, narirutin, naringin, hesperidin, neohesperidin, quercetin, naringenin, kaempferol and hesperetin) with reported effects in the treatment of depression and cardiac and neurodegenerative diseases was performed. An RP-ultrahigh-performance liquid chromatography (UHPLC)-ultraviolet method for analyte separation and determination was successfully developed and validated for a ZORBAX Eclipse Plus C18 RRHD analytical column. Separation was carried out in gradient elution mode with acetonitrile and water modified with 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy075DOI Listing
July 2018
1 Read

Speciation of Arsenic in Ground Water Samples by Ion Exchange Chromatography with Improved Sample Preparation Technique.

J Chromatogr Sci 2018 Oct;56(9):853-857

Metrohm India Limited, Chennai, India.

Arsenic exists in the form of various chemical species differing in their physicochemical behavior, toxicity, bioavailability and biotransformation. The determination of arsenic species is an important issue for environmental, clinical and food chemistry. However, differentiation of these species is a quite complex analytical task. Read More

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http://dx.doi.org/10.1093/chromsci/bmy076DOI Listing
October 2018
4 Reads

A Simple Method for Peak Alignment Using Relative Retention Time Related to an Inherent Peak in Liquid Chromatography-Mass Spectrometry-Based Metabolomics.

J Chromatogr Sci 2018 Jul 31. Epub 2018 Jul 31.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.

Retention time (RT) shifts are inevitable in liquid chromatography-mass spectrometry (LC-MS)-based metabolomics studies. RT alignment, aligning peaks originating from the same feature to an identical RT, is a necessary step prior to multivariate data analysis. Many RT alignment software packages and various algorithms have been developed. Read More

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http://dx.doi.org/10.1093/chromsci/bmy074DOI Listing
July 2018
5 Reads

Validation of a rapid and simple high-performance liquid chromatography-electrospray ionization-mass spectrometry method for simultaneous analysis of 15 key chemicals in slimming foods and herbal products.

J Chromatogr Sci 2018 Nov;56(10):912-919

Healthy Food Evaluation Research Center, West China School of Public Health, Sichuan University, Chengdu, Sichuan, China.

A rapid and simple high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed for simultaneous analysis of 15 key chemicals in slimming foods and herbal products. These chemicals were strictly restricted adulterants (phenolphthalein, sibutramine, fenfluramine, clopamide, ephedrine, norpseudoephedrine, caffeine and phenformin) and physiologically relevant active components (l-carnitine, nuciferine, aloe-emodin, rhein, emodin, chrysophanol and physcion). In multi-day validations, intra- (n = 5) and inter-day precision (n = 3), limits of detection and limits of quantification for all analytes were 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy068DOI Listing
November 2018

Identification, Characterization and High-Performance Liquid Chromatography Quantification for Process-Related Impurities in Abiraterone Acetate Bulk Drug.

J Chromatogr Sci 2018 Oct;56(9):802-811

Department of Biological and Food Engineering College, Hubei University of Technology, No.28, Nanli Road, Hong-shan District, Wuchang, Wuhan, Hubei Province, PR China.

During the synthesis of abiraterone acetate bulk drug in some laboratory batches, two unreported impurities were detected by high-performance liquid chromatography analysis at levels ranging from 0.05 to 0.10% according to the United States Pharmacopeia method. Read More

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http://dx.doi.org/10.1093/chromsci/bmy065DOI Listing
October 2018
15 Reads

Determination of Phosphodiesterase Inhibitors Tadalafil, Roflumilast and Roflumilast N-Oxide Using LC-MS in Guinea Pig Plasma.

J Chromatogr Sci 2018 Nov;56(10):948-954

Department of Pharmacology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.

Phosphodiesterases are known as a super-family of 11 isoenzymes, which can exert various functions based on their organ distribution. In this work, a rapid and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry method was developed for quantification of tadalafil (phosphodiesterase five inhibitor), roflumilast (RF) (phosphodiesterase four inhibitor) and its active metabolite, RF N-oxide in guinea pig plasma. Chromatographic separation was carried out on UPLC BEH C18 column (2. Read More

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http://dx.doi.org/10.1093/chromsci/bmy072DOI Listing
November 2018
1 Read

A Rapid and Precise Liquid Chromatographic Method for Simultaneous Determination of Alpha Lipoic Acid and Docetaxel in Lipid-Based Nanoformulations.

J Chromatogr Sci 2018 Nov;56(10):888-894

Industrial Research Laboratory (IRL), Department of Pharmacy, Birla Institute of Technology and Science (BITS-PILANI), Pilani, Rajasthan, India.

Combinational drug delivery successfully merges the benefits of nanotechnology and combination therapy by providing diversity to improve the carrier properties and better control over tailoring them as per the need of cancer treatment. A combination of conventional chemotherapeutic agent; docetaxel (DTX) and antioxidant agent; alpha lipoic acid (ALA) which acts by preventing metastasis may fulfill idealness of control and targeted drug delivery against breast cancer. The objective of the current study is to develop a reverse-phase HPLC-UV method for simultaneous determination of DTX and ALA in lipid-based nanoformulations. Read More

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http://dx.doi.org/10.1093/chromsci/bmy064DOI Listing
November 2018
17 Reads
1.360 Impact Factor

HPLC Method for Simultaneous Determination of Bisphenol-A-Diglycidyl Ether and Some of Its Reaction Products in Canned Foods Using Photodiode Array Detector.

J Chromatogr Sci 2018 Nov;56(10):920-932

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt.

Gradient reversed-phase high-performance liquid chromatography with photodiode array detection was used for separation, detection and quantification of bisphenol-A-diglycidyl ether (BADGE) and some of its reaction products, namely, BADGE·HCl·H2O, BADGE·H2O and BADGE·2HCl in pure form and in canned foods, where canned beans and tuna were used as representatives of aqueous and oil-in-water food matrices, respectively. The proposed method had a linear range of 0.01-0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy069DOI Listing
November 2018
3 Reads

Analysis of Fatty Acids in Trichosanthes kirilowii Maxim by MSPD and Fluorescence Derivatization.

J Chromatogr Sci 2018 Nov;56(10):941-947

Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Department of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong, China.

The extraction and analysis of free fatty acids (FFAs) in the whole natural plants are reported. The aminopropyl-functionalized SBA-15 was designed to increase the efficiency of the matrix solid-phase dispersion (MSPD) extraction method. MSPD extraction was performed using to extract FFAs via acid-base interactions. Read More

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http://dx.doi.org/10.1093/chromsci/bmy071DOI Listing
November 2018
7 Reads

Simultaneous Determination of Quercitrin, Afzelin, Amentoflavone, Hinokiflavone in Rat Plasma by UFLC-MS-MS and Its Application to the Pharmacokinetics of Platycladus orientalis Leaves Extract.

J Chromatogr Sci 2018 Nov;56(10):895-902

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.

Leaves of Platycladus orientalis have been used as blood cooling and homeostatic therapy for thousands of years in traditional Chinese medicine. Emerging evidences of modern pharmacology have proved flavonoids as the key elements responsible for the efficacies. However, there has been no report on pharmacokinetic study of the flavonoids from Platycladus orientalis leaves extract. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/chromsci/bmy066DOI Listing
November 2018
5 Reads

Supported liquid extraction and LC-MS-MS determination of iloperidone and olanzapine in rat plasma: Application to a pharmacokinetic study.

J Chromatogr Sci 2018 Nov;56(10):879-887

Department of S&H, Vignan's Foundation For Science, Technology and Research University (VFSTRU), Vadlamudi, Guntur, Andhra Pradesh, India.

A sensitive, selective rapid bioanalytical assay method was developed and quantification of iloperidone (ILP) and olanzapine (OLZ) in rat plasma was done by mass spectrometry. Systematic sample preparation and extraction procedure were carried out by supported liquid extraction using dichloromethane to extract both the eluents (ILP and OLZ) from rat plasma samples. The extorted samples were injected on a selective Waters XTerra® C18 reverse-phase bonded column (250 × 4. Read More

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http://dx.doi.org/10.1093/chromsci/bmy063DOI Listing
November 2018
1 Read

Reversed-Phase High-Performance Liquid Chromatography and High-Performance Thin-layer Liquid Chromatography Methods for Simultaneous Determination of Theophylline, Guaifenesin and Guaifenesin Impurity (Guaiacol) in Their Bulk Powders and in Dosage Form.

J Chromatogr Sci 2018 Oct;56(9):846-852

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo, Egypt.

Two simple, rapid chromatographic methods were developed for the simultaneous determination of Theophylline (THP), Guaifenesin (GUI) and Guaifenesin impurity namely Guaiacol (GUA). The first method is an isocratic reversed-phase high-performance liquid chromatography (RP-HPLC) method, where separation of THP, GUI and GUA was achieved on C18 column using methanol:water (containing 0.1% triethylamine):acetonitrile (30:60:10, by volume) as a mobile phase with a flow rate of 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy062DOI Listing
October 2018
1 Read

Liquid Chromatographic Enantioseparation of Some Fluoroquinoline Drugs Using Several Polysaccharide-Based Chiral Stationary Phases.

J Chromatogr Sci 2018 Oct;56(9):835-845

Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Center, Dokki, Giza, Egypt.

The enantioseparation of three fluoroquinoline antibacterial drugs, namely, flumequine, ofloxacin and lomefloxacin using high-performance liquid chromatography was optimized on seven polysaccharide-derived chiral stationary phases, namely, Chiralpak® IB, chiralpak® IA, Chiralpak® AD, Chiralcel® OJ, Chiralcel® OD, Chiralcel® OD-H and Chiralcel® OZ-3 and applying different mobile phases in isocratic mode is described. The role of addition of organic additives was also investigated. A baseline separation of flumequine, ofloxacin and lomefloxacin enantiomers was achieved. Read More

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http://dx.doi.org/10.1093/chromsci/bmy061DOI Listing
October 2018
1 Read
1.360 Impact Factor

Pressurized Liquid Extraction of Brazilian Coal Followed by the Extracts Characterization by Gas Chromatography Coupled to Mass Spectrometry.

J Chromatogr Sci 2018 Sep;56(8):761-769

University of Sao Paulo, Institute of Chemistry of São Carlos, São Carlos, SP, Brazil.

This work reports a study on the use of Pressurized Liquid Extraction (PLE) for the selective extraction of organic compounds (aliphatic hydrocarbons - AHs, polycyclic aromatic hydrocarbons - PAHs and polycyclic aromatic compounds - PACs) from high-ash Brazilian coal. A homemade PLE system was built and solvents of different polarities (hexane, toluene and ethanol) were employed to extract the organic compounds from coal samples at a pressure of 10 MPa, under temperatures of 80°C and 200°C and extraction times up to 120 min. The selectivity of the extraction process was evaluated by a well-established methodology employed for the characterization of coal extracts (Preparative Liquid Chromatography - PLC 8). Read More

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http://dx.doi.org/10.1093/chromsci/bmy051DOI Listing
September 2018
1 Read

Development and Validation of the Chiral Liquid Chromatography Method for Separation of Enantiomeric Impurity in Novel Oxazolidinone Antibacterial Agent WCK 4086.

J Chromatogr Sci 2018 Oct;56(9):789-793

Wockhardt Research Center, D-4, MIDC, Chikalthana, Aurangabad, India.

A highly stereo-specific liquid chromatographic method was developed and validated for the quantification of enantiomeric impurity (R-enantiomer) in novel oxazolidinone antibacterial agent (WCK 4086), a drug substance. The separation was achieved on Chiralpak AD-H (amylose-based chiral stationary phase) using a mobile phase consisting of n-hexane:2-propanol:methanol:trifluoroacetic acid (80:10:10:0.4, v/v/v/v) at a flow rate of 1. Read More

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https://academic.oup.com/chromsci/article/56/9/789/5040326
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http://dx.doi.org/10.1093/chromsci/bmy052DOI Listing
October 2018
5 Reads

Simultaneous Determination of Paracetamol and Chlorzoxazone in Their Combined Pharmaceutical Formulations by Reversed-phase Capillary Liquid Chromatography Using a Polymethacrylate Monolithic Column.

J Chromatogr Sci 2018 Oct;56(9):819-827

Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.

Recently, analytical separation techniques have the potential toward green approaches to reduce the environmental impact. This study focuses on the development of an analytical method for the determination of paracetamol and chlorzoxazone in their pharmaceutical combination. The separation was achieved using a home-made capillary column (0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy058DOI Listing
October 2018
4 Reads

GC-MS and GC-IR Analyses of the Methoxy-1-n-pentyl-3-(1-naphthoyl)-indoles: Regioisomeric Designer Cannabinoids.

J Chromatogr Sci 2018 Oct;56(9):779-788

Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, AL, USA.

The indole ring regioisomeric methoxy-1-n-pentyl-3-(1-naphthoyl)-indoles represent indole ring-substituted analogs of the synthetic cannabinoid JWH-018. The electron ionization mass spectra show equivalent regioisomeric major fragments resulting from cleavage of the groups attached to the central indole nucleus. The characteristic (M-17)+ fragment ion at m/z 354 resulting from the loss of OH group is significant in the mass spectra of all four compounds. Read More

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http://dx.doi.org/10.1093/chromsci/bmy059DOI Listing
October 2018
1 Read
1.360 Impact Factor

Quantitative Assessment of Poorly Soluble Anticoagulant Rivaroxaban by Microemulsion Electrokinetic Chromatography.

J Chromatogr Sci 2018 Aug;56(7):650-655

Laboratório de Controle de Qualidade Farmacêutico, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, lab 402, Porto Alegre, Brazil.

Microemulsion electrokinetic chromatography (MEEKC) is an electrophoretic methodology based on the separation of compounds by a microemulsionated electrolyte. There are few options for the evaluation of the stability and content of the oral anticoagulant rivaroxaban (RIV) in pharmaceutical formulations. RIV has low water solubility and undergoes ionization only under restricted pH conditions (pH < 1 or pH > 13), thus, hindering the application of free zone capillary electrophoresis as an analytical method. Read More

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http://dx.doi.org/10.1093/chromsci/bmy036DOI Listing
August 2018
2 Reads

A LC-MS-MS assay for simultaneous determination of two glycopeptides and two small molecule compounds in human plasma.

J Chromatogr Sci 2018 Oct;56(9):828-834

The First Hospital of Jilin University, Changchun, Jilin, China.

In this study, a novel and high-throughput liquid chromatography-tandem mass spectrometric (LC-MS-MS) assay was developed and validated for simultaneous determination of two glycopeptides (vancomycin, teicoplanin) and two small molecule compounds (meropenem, voriconazole) in human plasma. Only 50 μL of human plasma is used to quantify these four drugs simultaneously at clinical concentration levels. After a relative simple protein precipitation, the supernatant was then diluted with mobile phase acetonitrile: 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy060DOI Listing
October 2018
8 Reads

Pharmacokinetic and metabolic studies of ginsenoside Rb3 in rats using RRLC-Q-TOF-MS.

J Chromatogr Sci 2018 Jul;56(6):480-487

Jilin Ginseng Academy, Changchun University of Chinese Medicine, 1035 Boshuo Road, Changchun, China.

Ginsenoside Rb3 is one of major ginsenosides in Panax ginseng with effect on cardio-vascular and central nervous system. The aim of this study is to develop a rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method for pharmacokinetic study of ginsenoside Rb3 and simultaneous determination of metabolites in rats. The results showed that the concentration-time profile of ginsenoside Rb3 conformed to a two-compartment pharmacokinetic model after intravenous administration at the dosage of 2. Read More

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http://dx.doi.org/10.1093/chromsci/bmy019DOI Listing
July 2018
6 Reads