3,412 results match your criteria Journal of chromatographic science[Journal]


Purification of Flavonolignan Diastereoisomers from Arenaria kansuensis by Two-Dimensional Liquid Chromatography Combined with Solid-Phase Extraction.

J Chromatogr Sci 2019 Feb 7. Epub 2019 Feb 7.

Northwest Institute of Plateau Biology, College of Life Science, Chinese Academy of Sciences, Xining, Qinghai, P.R. China.

Herbal plants are significant for the reason that they have a great potential in discovering drug precursors. However, how to purify compounds with higher purity from them is a question which needs to be discussed. In present study, an offline 2D reversed-phase (RP) preparative liquid chromatography coupled with solid-phase extraction (SPE) method was successfully developed for the separation of flavonolignan diastereoisomers from Arenaria kansuensis. Read More

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http://dx.doi.org/10.1093/chromsci/bmz010DOI Listing
February 2019
1 Read
1.363 Impact Factor

Development and Validation of High-Performance Thin-layer Chromatographic Method for Quantification of Berberine in Rhizomes of Coptis teeta Wall, an Endangered Species Collected from Arunachal Pradesh, India.

J Chromatogr Sci 2019 Feb 7. Epub 2019 Feb 7.

Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.

The high polarity of the protoberberine alkaloids present in Coptis teeta has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance thin-layer chromatography for quantification of berberine. The separation was achieved in a solvent system consisting of butanol:ethyl acetate:formic acid:water by volume on TLC aluminum plate precoated with silica gel 60 F254. Read More

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http://dx.doi.org/10.1093/chromsci/bmz009DOI Listing
February 2019

Simultaneous Determination of Anti-diabetic Drugs in Their Combined Dosage Forms Using HPLC: An Experimental Design Approach.

J Chromatogr Sci 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India.

A liquid chromatographic method has been established for the separation of five presently existing anti-diabetic drugs-metformin, pioglitazone, glibenclamide, glimepiride and repaglinide using design of experiments methodology. The main objective of this method is to identify the suitable conditions for the adequate separation of these five drugs with minimal analysis time. Experiment design is one of the Quality by design approaches, which incorporates multifaceted combinations of independent variables and their interactions during the optimization of analytical methods for the determination of drugs. Read More

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http://dx.doi.org/10.1093/chromsci/bmz003DOI Listing
February 2019
1 Read

Enantioseparation of Synthetic Cathinones Enantiomers with Tertiary Amine Structure in Urine and Plasma.

J Chromatogr Sci 2019 Feb 7. Epub 2019 Feb 7.

Chemistry Department, College of science, United Arab Emirates University, Al-Ain, UAE.

A sensitive and selective method for detection and quantitation of the enantiomers of 18 synthetic cathinones with tertiary amine structure using HPLC-UV-VIS has been developed. Two chiral columns, Astec Cellulose DMP and Amylose-based Chiralpak AS-H, have been examined separately. Mobile phase composed of hexane, isopropanol and triethylamine (99. Read More

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http://dx.doi.org/10.1093/chromsci/bmz008DOI Listing
February 2019

Synthesis of High Purified Tulobuterol and Its Study of the Organic Impurities.

J Chromatogr Sci 2019 Feb 4. Epub 2019 Feb 4.

Beijing Key Laboratory of Drug Delivery and Novel Formulation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

The synthetic condition of tulobuterol was optimized to gain lower impurity content. Two intermediates were analyzed, and three degradation impurities were isolated using preparative liquid chromatography for the first time and subsequently characterized by various techniques. Possible degradation impurities were deduced by an oxidative mechanism. Read More

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http://dx.doi.org/10.1093/chromsci/bmy083DOI Listing
February 2019
1 Read

Rapid Determination of Adenosine in Cordyceps by Online Extraction HPLC.

J Chromatogr Sci 2019 Feb 4. Epub 2019 Feb 4.

Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co., LTD, Dongguan, Guangdong, China.

In this study, determination of adenosine in Cordyceps was accomplished by an online pressurized liquid extraction HPLC, and a poroshell column was employed for rapid HPLC separation. The Cordyceps sample powder was filled into a hollow guard column and extracted by thermal and pressurized water (75°C, 150 bar) for 3 min. Chromatographic separation was conducted on a poroshell 120 SB-Aq (4. Read More

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http://dx.doi.org/10.1093/chromsci/bmz007DOI Listing
February 2019

Development and Validation of Simple LC-MS-MS Assay for the Quantitative Determination of Ticagrelor in Human Plasma: its Application to a Bioequivalence Study.

J Chromatogr Sci 2019 Jan 31. Epub 2019 Jan 31.

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, Republic of Korea.

A sensitive, rapid, reproducible and reliable high-performance liquid chromatography-electrospray ionization tandem mass spectrometric method has been developed and fully validated for the determination of ticagrelor in human plasma using ticagrelor-d7 as an internal standard (IS) after one-step liquid-liquid extraction with ethyl acetate. Ticagrelor and IS were detected in the multiple reaction monitoring mode at m/z transition 523.4 > 127. Read More

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http://dx.doi.org/10.1093/chromsci/bmz001DOI Listing
January 2019
1 Read

Estimation of C2H5-Benzenes in Refinery Streams.

J Chromatogr Sci 2019 Jan 31. Epub 2019 Jan 31.

Indian Oil Corporation Limited, Research and Development Centre, Sector 13, Faridabad, Haryana, India.

A simple and direct gas chromatography-mass spectrometry method for routine estimation of C2 H5-alkylbenzenes (or C2-alkylbenzenes), namely, ethylbenzene and m/p- and o-xylenes in the boiling range of 80°C-400°C, including diesel range streams using deuterated ethylbenzene as internal standard has been described. Estimation of C2-alkylbenzenes in feeds and products is important for monitoring the reaction kinetics in certain secondary processes which involve conversion of low value, aromatic-rich diesel range boiling streams to produce aromatic-based petrochemical feedstocks upto xylenes. The method developed takes advantage of chromatographic separation and resolution masses of fragment ions of the compounds of interest for estimation. Read More

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http://dx.doi.org/10.1093/chromsci/bmz002DOI Listing
January 2019
1 Read

A Validated Ultra-Performance Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometric Method for Fast Determination of Losartan in Rabbit Plasma.

J Chromatogr Sci 2019 Jan 22. Epub 2019 Jan 22.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh-11451, Saudi Arabia.

A rapid UPLC-MS-MS method was developed and validated for determination of losartan in rabbit plasma. Protonated adducts of losartan and eprosartan (IS) were monitored in multiple reaction monitoring mode. Molecular masses of daughter species of losartan were m/z 423. Read More

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http://dx.doi.org/10.1093/chromsci/bmy114DOI Listing
January 2019
7 Reads

Comparative Study of Quinolines and Tetrahydroquinolines Sorption on Various Sorbents from Water-Acetonitrile Solutions.

J Chromatogr Sci 2019 Jan 7. Epub 2019 Jan 7.

Department of Physical Chemistry and Chromatography, Samara National Research University Named after Academician S.P. Korolev, 34 Moskovskoye Shosse, Samara, Russia.

The retention of 16 quinoline and tetrahydroquinoline derivatives was investigated under liquid chromatography conditions using porous graphitized carbon (PGC), octadecyl silica (ODS) and hypercrosslinked polystyrene (HCLP) stationary phases. For most of the analytes, retention on PGC was greater than on ODS, while retention on HCLP was even greater than on both ODS and PGC. The non-linearity of retention dependencies on acetonitrile content in the eluent was observed for compounds containing carboxy, hydrazo and methoxy groups. Read More

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http://dx.doi.org/10.1093/chromsci/bmy111DOI Listing
January 2019

Validated Chromatographic Methods for Simultaneous Determination of Calcipotriol Monohydrate and Betamethasone Dipropionate in the Presence of Two Dosage Form Additives.

J Chromatogr Sci 2019 Jan 7. Epub 2019 Jan 7.

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini Post, Cairo, Egypt.

Two chromatographic methods were developed, optimized and validated for simultaneous determination of calcipotriol monohydrate (CPM) and betamethasone dipropionate (BMD) in the presence of two dosage form additives named; butylated hydroxytoluene (BHT) and alpha-tocopherol (TOCO). The proposed methods were accurate, sensitive and specific. The first method based on using aluminum thin-layer chromatographic plates precoated with silica gel GF254 as a stationary phase and chloroform-ethyl acetate-toluene (5:5:3, by volume) as a developing system. Read More

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http://dx.doi.org/10.1093/chromsci/bmy112DOI Listing
January 2019

With or Without Internal Standard in HPLC Bioanalysis. A Case Study.

J Chromatogr Sci 2019 Mar;57(3):243-248

Departament of Pharmaceutical Sciences, Faculty of Pharmacy, University of Medicine and Pharmacy from Tîrgu Mures, Gheorghe Marinescu street 38, Tîrgu Mures, Romania.

The mandatory strategy of using internal standard in HPLC is still controversial. Despite that the introduction of internal standard methodology in the early stage of development of HPLC technology was used to improve method accuracy and precision, there are still practical situations in which a simple external standard quantification is adequate. The aim of the study is to compare the determination of meloxicam (MXC) in human plasma by HPLC with or without using an internal standard according to some key points related to the reason of introducing the internal standardization such as the reducing of sample preparation errors or variability for low injection volumes. Read More

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http://dx.doi.org/10.1093/chromsci/bmy106DOI Listing
March 2019
1 Read

High-Performance Thin-Layer Chromatography Method for Simultaneous Determination of Antipsychotic and Medicinally Important Five β-Carboline Alkaloids.

J Chromatogr Sci 2019 Jan 4. Epub 2019 Jan 4.

Department of Botany, Savitribai Phule Pune University, Pune, MH, India.

This is the first report on the development and validation of high-performance thin-layer chromatography (HPTLC) method for simultaneous analysis of five antipsychotic and medicinally important β-carboline alkaloids (βCAs), namely, harmalol, harmaline, harmine, harmane and norharmane. These βCAs occurs in both plant and animal system including human being. In the present investigation, their best separation was achieved using an optimized mobile phase, chloroform: methanol: glacial acetic acid (7. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy113DOI Listing
January 2019
2 Reads

Fingerprint Analysis and Multi-Component Determination of Ribonucleic Acid for Injection II Recipe by HPLC-DAD and LC-ESI-MS Methods.

J Chromatogr Sci 2019 Mar;57(3):238-242

Center of Research & Development, Changchun University of Chinese Medicine, No 1035, Boshuo Road Changchun, Jilin, China.

Using high-performance liquid chromatography (HPLC), a quantitative method was developed to control the quality of ribonucleic acid for injection II (RA-II), which is used as an anti-cancer drug clinically in China. Using nuclease enzyme and under optimal hydrolysis conditions, the unstable RNA was hydrolyzed into stable nucleosides and nucleotides, which were easily detected by HPLC with diode array detection. Furthermore, by analyzing HPLC chromatograms of 10 batches of samples, six common peaks, namely, cytosine nucleotide, uracil nucleotide, adenine nucleotide, guanine nucleotide, guanosine and adenine, were identified by a HLPC coupled with electrospray ionization mass spectrometry. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy105DOI Listing
March 2019
7 Reads

Preparative separation of quercetin, ombuin and kaempferide from Gynostemma pentaphyllum by high-speed countercurrent chromatography.

J Chromatogr Sci 2019 Mar;57(3):265-271

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, P. R. China.

An efficient method for the preparative separation of quercetin, ombuin and kaempferide from Gynostemma pentaphyllum by high-speed countercurrent chromatography (HSCCC) was successfully developed for the first time. The extraction conditions were optimized by an orthogonal array design L9 (34), while quercetin, ombuin and kaempferide were efficiently released from rutin, ombuoside and kaempferide glucoside by hydrochloric acid hydrolysis, respectively. Quercetin was purified by HSCCC with two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (4:5:4:5, v/v) in a single run. Read More

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http://dx.doi.org/10.1093/chromsci/bmy110DOI Listing

Optimized liquid chromatography-tandem mass spectrometry for Otaplimastat quantification in rat plasma and brain tissue.

J Chromatogr Sci 2019 Mar;57(3):258-264

Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, South Korea.

An optimized liquid chromatography-tandem mass spectrometry method for simple and sensitive quantification of Otaplimastat in rat plasma and brain tissue was developed and validated. Protein precipitation with acetonitrile was selected for sample preparation method based on recovery and matrix effect. The chromatographic separation of the sample was performed on a reverse-phase AQ column with an isocratic mobile phase consisting of 10 mM ammonium acetate (pH 4. Read More

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http://dx.doi.org/10.1093/chromsci/bmy109DOI Listing
March 2019
3 Reads

Development and Validation of Stability Indicating UPLC-PDA/MS for the Determination of Imiquimod and its Eight Related Substances: Application to Topical Cream.

J Chromatogr Sci 2019 Mar;57(3):249-257

Dr. Reddy's Laboratories Limited, Bachupally, Survey No. 42, 45 & 46, Qutubullapur Mandal Hyderabad, India.

A stability indicating analytical method for imiquimod and its related impurities was developed by ultra-pressure liquid chromatography (UPLC) using design of experiments. This method could quantify imiquimod and all its eight known related impurities in a single run. The optimum separation was achieved on reverse phase Acquity UPLC column (2. Read More

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http://dx.doi.org/10.1093/chromsci/bmy108DOI Listing
March 2019
5 Reads

Response Surface Methodology of Pre-Concentration of Chorophenols from Seawater Samples by Molecularly Imprinted Stir Bar Sorptive Extraction Combined with HPLC: Box-Behnken Design.

J Chromatogr Sci 2019 Mar;57(3):279-289

Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, Chabahar, Iran.

A novel molecularly imprinted polymer coated stir bar was prepared using 2-chlorophenol (2-CP) as template molecule and used for selective extraction of phenolic compounds including chlorophenols (CPs) directly from seawater samples. Various parameters affecting extraction have been evaluated using one variable-at-a-time and Box-Behnken design. A three-level Box-Behnken experimental design by six factors, which combined the response surface methodology, was used to optimize the selective extraction of the CPs. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy107DOI Listing
March 2019
1 Read

Modeling Retention Behavior on Analysis of Hallucinogenic Mushrooms Using Hydrophilic Interaction Liquid Chromatography.

J Chromatogr Sci 2019 Mar;57(3):230-237

Department of Drug and Arson Investigation, Hungarian Institute for Forensic Sciences, Mosonyi Street 9, Budapest, Hungary.

The goal of this work was to investigate and compare the selectivity of three different hydrophilic interaction liquid chromatography (HILIC) charge modulated amide columns, iHILIC®-Fusion, iHILIC®-Fusion(+) and iHILIC®-Fusion(P), for analysis of compounds in hallucinogen mushrooms. An extract of a truffle-like fungus containing psilocin, psilocybin and baeocystin was chosen as test material. Three different modeling methods were applied to describe the retention times of constituents in isocratic separation mode as a function of mobile phase composition, pH and temperature. Read More

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http://dx.doi.org/10.1093/chromsci/bmy104DOI Listing
March 2019
1 Read

A High Throughput Three-step Ultra-performance Liquid Chromatography Tandem Mass Spectrometry Method to Study Metabolites of Atractylenolide-III.

J Chromatogr Sci 2019 Feb;57(2):163-176

Department of Natural Medicine Chemistry, Institute of Pharmacy, Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Avenue, Nanchang, PR China.

Atractylodes macrocephala Koidz (AMK) is a traditional Chinese medicine widely used in the treatment of various diseases, especially spleen deficiency. As the principle active constituents of AMK, however, the metabolites of Atractylenolide-III (A-lactone-III) have not been identified in rats yet. In this study, a three-step high throughput method based on UHPLC-Q-TOF-MS-MS was developed to profile and characterize the metabolites of A-lactone-III in rat feces, urine and plasma. Read More

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http://dx.doi.org/10.1093/chromsci/bmy098DOI Listing
February 2019

Simultaneous Quantification of Benznidazole and Posaconazole by HPLC-DAD Using QbD Approach.

J Chromatogr Sci 2019 Feb;57(2):156-162

Department of Pharmaceutical Sciences, Federal University of Pernambuco, PE, Brazil.

Objective: To develop an analytical method to simultaneous quantification of benznidazole (BNZ) and posaconazole (POS) by high-performance liquid chromatography with diode-array detection (HPLC-DAD) using design of experiments.

Methods: Percentages of organic phase, buffer pH and flow rates of mobile phase were selected as independent variables by full factorial design (33), totaling 27 experiments. Significant factors were evaluated using factorial analysis of variance with 95% confidence level. Read More

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http://dx.doi.org/10.1093/chromsci/bmy097DOI Listing
February 2019
2 Reads

Development and Validation of LC-MS-MS Assay for the Determination of the Emerging Alkylating Agent Laromustine and Its Active Metabolite in Human Plasma.

J Chromatogr Sci 2019 Mar;57(3):195-203

Metastagen, Inc., Wilmington, DE, USA.

The objective of this study was to validate a method for the determination of laromustine (VNP40101M) and short-lived its active metabolite (VNP4090CE) that has a half-life in human blood of <90 s in human plasma by liquid chromatography (LC) with tandem mass spectrometric (MS/MS) detection. We overcome the stability dilemma by acidified the human plasma with citric acid. Laromustine "breaks" down on the source of mass spectrometry to give m/z 249 which is the same m/z for VNP4090CE. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy100DOI Listing
March 2019
5 Reads

HPTLC method for Simultaneous Determination of Norfloxacin and Tinidazole in presence of Tinidazole Impurity.

J Chromatogr Sci 2019 Jan;57(1):81-86

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., Beni-Suef, Egypt.

Sensitive, selective and accurate high-performance thin layer chromatographic HPTLC method for quantitative determination of Norfloxacin (NF), Tinidazole (TZ) and 2-Methyl-5-nitroimidazole (MNZ) as potential impurity of Tinidazole is developed and validated in the presented work. Calibration curves were linear over the concentration ranges of 0.4-2. Read More

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http://dx.doi.org/10.1093/chromsci/bmy085DOI Listing
January 2019
3 Reads

Separation and Characterization of Unknown Impurities and Isomers in Cefminox Sodium and Study of the Forming Mechanisms of Impurities by Liquid Chromatography Coupled with Ion Trap/Time-Of-Flight Mass Spectrometry.

J Chromatogr Sci 2019 Mar;57(3):204-212

Zhejiang University of Technology, College of Pharmacy, Hangzhou, China.

Thirteen unknown impurities and isomers in cefminox sodium were separated and characterized by liquid chromatography coupled with high-resolution ion trap/time-of-flight mass spectrometry (LC-IT-TOF-MS) with the positive mode of electrospray ionization (ESI) method. New HPLC-gradient elution method was developed for the detection of impurities in cefminox sodium. And the ESI ion trap multiple-stage tandem mass spectrometry had been applied successfully to the direct investigation of impurities and isomers in cefminox sodium. Read More

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http://dx.doi.org/10.1093/chromsci/bmy101DOI Listing
March 2019
5 Reads

Stability-Indicating RP-UPLC Method for Simultaneous Determination of Azilsartan Medoxomil and Chlorthalidone in Tablets in the Presence of Its Degradation Products.

J Chromatogr Sci 2019 Mar;57(3):213-219

Research and Development Department, Eva Pharma for Pharmaceutical and Medical Appliances, Giza, Egypt.

Azilsartan Medoxomil (AZL) angiotensin II receptor blocker and chlorthalidone (CLT) were determined by ultraperformance liquid chromatography (UPLC) method in their combined dosage form, they were both subjected to forced degradation studies under extensive stress conditions. The method is a stability indicating by resolving the investigated drugs from their degradation products. Moreover, the degradation products for both drugs obtained from forced degradation were subjected to LC-MS for structure elucidation. Read More

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http://dx.doi.org/10.1093/chromsci/bmy102DOI Listing
March 2019
17 Reads

Application of Dopant-Assisted Atmospheric Pressure Photoionisation HPLC-MS Method for the Sensitive Determination of Polycyclic Aromatic Hydrocarbons in Dark Chocolate.

J Chromatogr Sci 2019 Mar;57(3):220-229

Institute of Food Safety, Animal Health and Environment "BIOR", Lejupes Street 3, Riga, Latvia.

Multiple food research studies have shown that the polycyclic aromatic hydrocarbon (PAHs) are frequently found in processed cocoa products and chocolate. In a present study a method based on dopant-assisted atmospheric pressure photoionisation (DA-APPI) combined with a liquid chromatography/high-resolution mass spectrometer (HPLC-HRMS) for high-sensitivity analysis of four EU marker PAHs in dark chocolate samples was developed and fully validated according to the performance criteria set in EU guidelines. PAHs detection was achieved by HRMS in positive electrospray ionization mode with toluene used as a dopant to enhance the ionization efficiency of non-polar PAHs. Read More

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http://dx.doi.org/10.1093/chromsci/bmy103DOI Listing
March 2019
2 Reads

A High-Performance Liquid Chromatography Method for Determination of Genotoxic Impurity Hydroxylamine in Drug Substances.

J Chromatogr Sci 2019 Jan;57(1):63-70

Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, India.

Hydroxylamine (NH2OH) is widely used in pharmaceutical intermediates and final drug substances synthesis. Since hydroxylamine is a well-known genotoxic impurity compound that needs to be controlled down to ppm level in pharmaceutical compounds. It is very difficult to detect using conventional analytical techniques due to its physical-chemical properties like the lack of chromophore, low molecular weight, the absence of carbon atom and high polarity. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy082DOI Listing
January 2019
19 Reads

HPTLC-Densitometric Method for Determination of Ascorbic Acid, Paracetamol and Guaifenesin in Presence of Their Toxic Impurities.

J Chromatogr Sci 2019 Feb;57(2):149-155

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., Beni-Suef, Egypt.

Ascorbic acid (ASC), paracetamol (PAR) and guaifenesin (GUF) are co-formulated together as analgesic and expectorant effervescent powder. This work describes a selective stability indicating HPTLC-densitometric method which is the first developed stability indicating one for chromatographic separation of the three studied drugs in presence of paracetamol toxic impurity [4-aminophenol (4-AP)] and guaifenesin related substance [impurity, (guaiacol) (GUC)]. The chromatographic separation was carried out on HPTLC 60F254 plates using chloroform-acetone-trifluoroacetic acid (6. Read More

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http://dx.doi.org/10.1093/chromsci/bmy096DOI Listing
February 2019

Green Tailoring With Water of Choline Chloride Deep Eutectic Solvents for the Extraction of Polyphenols From Palm Samples.

J Chromatogr Sci 2019 Mar;57(3):272-278

College of Pharmaceutical Science, Hebei University, 180 Wusi East Road, Baoding, China.

To address the problem of high viscosity of deep eutectic solvents (DESs), the viscosities of a series of choline chloride-based DESs and DESs-H2O were determined, and their extraction efficiencies were obtained for the extraction of polyphenols in palm samples. Tailoring the DESs by the addition of water successfully adjusted their viscosity from thousand to single-digit mPa s and aided the extraction of polyphenols from samples, by changing the hydrogen-bonding interactions of DESs. Among the compounds investigated, choline chloride-oxalic acid-water of DESs-H2O with various molar ratios with 1:0. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy099DOI Listing
March 2019
12 Reads

Non-Anthocyanin Phenolic Compounds in Açaí (Euterpe oleracea Mart.) Juice by Ultrahigh-Performance Liquid Chromatography-Diode Array Detector (UHPLC-DAD): A Multivariate Optimization.

J Chromatogr Sci 2019 Feb;57(2):139-148

Department of Food Science. Faculty of Food Engineering, University of Campinas (UNICAMP), Monteiro Lobato Street, Campinas, SP, Brazil.

Currently available high-performance liquid chromatographic (HPLC) methods for the analysis of non-anthocyanin compounds in açaí are time-consuming or employ complex and costly detection systems. Therefore, we hypothesized that by using ultrahigh-performance liquid chromatography interfaced with a diode array detector (UHPLC-DAD) in conjunction with multivariate optimization tools (response surface methodology, Derringer and Suich desirability) would give a simple, fast, effective and reliable method to determine eight phenolic acids and four flavonoids in açaí juice. Three independent variables were assessed: initial acetonitrile (ACN) concentration in the gradient elution, final ACN concentration at the end of the gradient and the linear time of the gradient elution. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/chromsci/bmy095DOI Listing
February 2019
2 Reads

Analysis of Triacylglycerols in Castor Oil Through Liquid Chromatography-Mass Spectrometry Based on Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry and Gas Chromatography-Mass Spectrometry.

J Chromatogr Sci 2019 Feb;57(2):108-115

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China.

Castor oil is a traditional Chinese medicine containing a chemically complex mixture of triacylglycerols (TAGs). Herein, the TAGs in castor oil were analyzed in detail. First, the fatty acid composition of castor oil was examined via methylated derivatisation followed by gas chromatography-mass spectrometry (GC-MS). Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy088DOI Listing
February 2019
4 Reads

A Validated GC-MS Method for the Determination of Genotoxic Impurities in Divalproex Sodium Drug Substance.

J Chromatogr Sci 2019 Feb;57(2):101-107

Aurobindo Pharma Limited Research Centre-II, Survey No: 71&72, Indrakaran Village, Kandi Mandal, Sangareddy, Telangana, India.

A specific GC-MS method has been developed, optimized and validated for the determination of five genotoxic impurities namely Methyl bromide (Me.-Br), Ethyl bromide (Et.-Br), Isopropyl bromide (Ipr. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy089DOI Listing
February 2019
2 Reads

Sensitivity Enhancement for Direct Injection Capillary Electrophoresis to Determine Morphine in Human Serum via In-capillary Derivatization.

J Chromatogr Sci 2019 Feb;57(2):177-185

Laboratory for Single Cell Mass Spectrometry, RIKEN Quantitative Biology Center, 6-2-3, Furuedai, Suita, Osaka, Japan.

Rapid and simple micellar electrokinetic chromatography (MEKC) with in-capillary derivatization and fluorescence detection has been developed to determine morphine in human serum. The sample was introduced into a background electrolyte (BGE) containing potassium ferricyanide, whereas morphine was oxidized into highly fluorescent product, pseudomorphine. Different parameters for derivatization and subsequent separation were systematically investigated for the analysis of morphine in serum. Read More

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http://dx.doi.org/10.1093/chromsci/bmy092DOI Listing
February 2019
2 Reads
1.363 Impact Factor

Simultaneous Determination of Thalidomide and Dexamethasone in Rat Plasma by Validated HPLC and HPTLC With Pharmacokinetic Study.

J Chromatogr Sci 2019 Feb;57(2):130-138

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St, Cairo, Egypt.

Two validated chromatographic methods have been developed for the simultaneous determination of thalidomide (THD) and dexamethasone (DEX) in rat plasma using paracetamol (PAR) as an internal standard (IS). Chromatographic analysis was achieved firstly by HPLC method on C18 column (150 × 4.6 mm2 i. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy094DOI Listing
February 2019
20 Reads

Matrix Solid-Phase Dispersion Combined with HPLC-DAD for Simultaneous Determination of Nine Lignans in Saururus chinensis.

J Chromatogr Sci 2019 Feb;57(2):186-193

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.

A simple and rapid method, based on matrix solid-phase dispersion (MSPD) and high-performance liquid chromatography (HPLC) was developed for simultaneous determination of nine lignans, including (-)-(7R,8R)-machilin D (Wang, C., Wang, P., Chen, X. Read More

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http://dx.doi.org/10.1093/chromsci/bmy090DOI Listing
February 2019
5 Reads

The Effect of Monolith Properties on the Digestion Performance of Monolith-Based Immobilized Enzyme Microreactor.

J Chromatogr Sci 2019 Feb;57(2):116-121

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Normal University, Cihu Road No. 11, Huangshi, China.

In this work, two kinds of monolith-based immobilized enzyme microreactors (IMER) were compared in terms of monolith properties and digestion performance. Two monoliths were prepared involving poly (tetraethoxysiLane-co-3-aminopropyltriethoxysilane) (poly (TEOS-co-APTES)) monolith and poly (N-acryloxysuccinimide-co-acrylamide-co-ethylene dimethacrylate) (poly (NAS-co-AA-co-EDMA)) monolith for covalent trypsin immobilization. A higher chromatographic permeability constant 66. Read More

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http://dx.doi.org/10.1093/chromsci/bmy091DOI Listing
February 2019
10 Reads

Simultaneous Determination of Ten Bioactive Components in Raw and Processed Radix Dipsaci by UPLC-Q-TOF-MS.

J Chromatogr Sci 2019 Feb;57(2):122-129

Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.

Radix Dipsaci is employed for treating bone fractures in traditional Chinese medicine. In the current, a quantitative approach was established for determination of ten bioactive components (loganic acid, loganin, 3,5-diCQA, 4-CQA, 3,4-diCQA, sweroside, dipsacussaponin B, dipsacoside A, asperosaponin V and asperosaponin VI) in raw and rice wine-sautéed Radix Dipsaci by using UPLC-Q-TOF-MS based approach. The sample handling procedure was optimized. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy093DOI Listing
February 2019
3 Reads

Quantitative Analysis of Polycyclic Aromatic Hydrocarbons at Part Per Billion Levels in Fish Oil by Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS).

J Chromatogr Sci 2019 Jan;57(1):87-92

Department of Chemistry and Biochemistry, Center for Academic Industry Partnership, Seton Hall University, 400 South Orange Avenue, South Orange, NJ, USA.

Headspace solid-phase microextraction (HS-SPME) was used in combination with gas chromatography-mass spectrometry (GC-MS) for the analysis of polycyclic aromatic hydrocarbons (PAH) at part per billion levels in fish oil samples collected from menhaden fish. The method was initially developed using fish oil from capsules spiked with a standard PAH mixture. The final HS-SPME-GC-MS method presented a linear range from 3 to 1,500 ng/g, with precision for most analytes <10% relative standard deviation. Read More

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http://dx.doi.org/10.1093/chromsci/bmy086DOI Listing
January 2019
1 Read

Dispersive Liquid-Liquid Microextraction of Famotidine and Nizatidine from Water Samples.

J Chromatogr Sci 2019 Jan;57(1):93-100

Institute of Chemistry, Biological-Chemical Department, University of Bialystok, Bialystok, Poland.

A new extraction method was developed for isolation of the antihistaminic drugs (histamine H2 receptor antagonists) famotidine (FMT) and nizatidine (NZT) from aqueous samples. A low solvent consumption method using dispersive liquid-liquid microextraction (DLLME) was applied for this purpose. The parameters of the isolation, namely, volume of extraction and disperser solvents, pH of sample and shaking time were optimized. Read More

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http://dx.doi.org/10.1093/chromsci/bmy087DOI Listing
January 2019
6 Reads

Atom-Type-Based AI Topological Indices for Artificial Neural Network Modeling of Retention Indices of Monomethylalkanes.

J Chromatogr Sci 2019 Jan;57(1):1-8

Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, I.R. Iran.

In this work, a combination of Xu and atom-type-based AI topological indices (TIs) were employed for quantitative structure-retention relationship (QSRR) study of monomethylalkanes (MMAs). A total of 196 temperature-programmed gas chromatographic retention indices corresponding to all C4-C30 MMAs on OV-1 stationary phase have been used in QSRR modeling. Results of the study showed that an artificial neural network (ANN) with 4-9-1 topology and Levenberg-Marquardt training algorithm can predict the retention indices with high degree of accuracy. Read More

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http://dx.doi.org/10.1093/chromsci/bmy081DOI Listing
January 2019
2 Reads

A Stability-Indicating UPLC Method for the Determination of Potential Impurities and Its Mass by a New QDa Mass Detector in Daclatasvir Drug Used to Treat Hepatitis C Infection.

J Chromatogr Sci 2019 Jan;57(1):44-53

Analytical R&D, Hetero Labs Ltd, Hyderabad, Telangana, India.

A stability-indicating ultraperformance liquid chromatographic method has been developed for the quantitative determination of degradation products and process-related impurities of daclatasvir in a pharmaceutical dosage form. Chromatographic separation was achieved on a polar Waters ACQUITY BEH phenyl 100 × 2.1 mm, 1. Read More

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https://academic.oup.com/chromsci/advance-article/doi/10.109
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http://dx.doi.org/10.1093/chromsci/bmy079DOI Listing
January 2019
22 Reads

An Alternative HPLC with Ultraviolet Detection for Determination of Piperaquine in Plasma.

J Chromatogr Sci 2019 Jan;57(1):27-32

Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Graduate Studies, Thammasat University (Rangsit Campus), Paholyothin Road, Pathumthani, Thailand.

A simple, sensitive, selective and reproducible method based on liquid chromatography was developed for the determination of piperaquine in human plasma samples. Sample preparation involved a single step liquid-liquid extraction by organic solvents (hexane: tert-butyl methylether at the ratio of 1:1, v:v). Piperaquine was separated from the internal standard mefloquine on a reversed-phase C18 column, with the mobile phase consisting of a mixture of acetonitrile and 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy077DOI Listing
January 2019
4 Reads

Development of a Simple, Highly Selective RP-LC Method for the Quantification of Diastereomers and Other Related Substances of Ezetimibe Using Multivariate Analysis.

J Chromatogr Sci 2019 Jan;57(1):71-80

IPDO, Dr. Reddy's Laboratories Ltd, Hyderabad, India.

A simple reverse phase method for the selective quantification of ezetimibe (EZM), its diastereomers and other related substances was developed. The method demonstrated an excellent separation between each of the 14 impurities (including diastereomers, specified impurities and degradation products) and EZM within a runtime of 45 min. The developed method was evaluated against the reported USP method, other literature methods found that none of them was able to separate/show the absence of all the diastereomers and degradation products. Read More

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http://dx.doi.org/10.1093/chromsci/bmy084DOI Listing
January 2019
6 Reads

Comparison of Chiral Recognition of Binaphthyl Derivatives with l-Undecyl-Leucine Surfactants in the Presence of Arginine and Sodium Counterions.

J Chromatogr Sci 2019 Jan;57(1):54-62

Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Howard University, 520 W Street NW, Washington, DC, USA.

In this study the chiral selectivity of l-undecyl-leucine (und-leu) for binapthyl derivatives was examined with the use of arginine and sodium counterions at pH's ranging from 7 to 11. The objective of this project was to investigate whether a cationic amino acid, such as arginine would achieve enhanced chiral selectivity when utilized as the counterion in the place of sodium in micellar electrokinetic chromatography. The data indicate that und-leu has significantly improved chiral selectivity toward 1,1'-binaphthyl-2,2'-diyl hydrogenphosphate (BNP) enantiomers in the presence of arginine counterions in comparison to sodium and that, at least in the case of this study, the enantiomeric form of the arginine did not appear to play a role in the chiral selectivity. Read More

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http://dx.doi.org/10.1093/chromsci/bmy080DOI Listing
January 2019
1 Read

Determination of Zoledronic Acid and Its Related Substances by High Performance Liquid Chromatography with Evaporative Light Scattering Detection.

J Chromatogr Sci 2019 Jan;57(1):33-43

Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, Turkey.

A method has been developed and validated for analysis of zoledronic acid (ZOL) and its related substances by ion-pair reversed-phase high performance liquid chromatography with evaporative light scattering detection (ELSD). Chromatographic separation was achieved with gradient elution by using a C18 column, mobile phase containing 12 mM ammonium acetate buffer and 35 mM n-pentylamine, whose pH value is 7.0, and 5% acetonitrile. Read More

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http://dx.doi.org/10.1093/chromsci/bmy078DOI Listing
January 2019
3 Reads

Simultaneous Determination of Dexpanthenol, Lidocaine Hydrochloride, Mepyramine Maleate and their Related Substances by a RP-HPLC Method in Topical Dosage Forms.

J Chromatogr Sci 2018 Nov;56(10):903-911

Hacettepe University Pharmaceutical Technology Department, Sihhiye, Ankara, Turkey.

The pharmaceutical combination of dexpanthenol (DPA), lidocaine hydrochloride (LIH) and mepyramine maleate (MAM) is used for their anti-allergic, anti-inflammatory, anti-pruritic, anesthetic and antiseptic properties. The present study was aimed to develop and validate a new, first and rapid high performance liquid chromatographic method for simultaneous determination of DPA, LIH and MAM in the presence of their stress-induced degradation products in pharmaceutical gel/fluigel formulations. The chromatographic separation was performed on an Inertsil ODS-3 V, 250 × 4. Read More

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http://dx.doi.org/10.1093/chromsci/bmy067DOI Listing
November 2018
12 Reads

Covalent Immobilization of Protein A on Chitosan and Aldehyde Double-Branched Chitosan as Biocompatible Carriers for Immunoglobulin G (Igg) Purification.

J Chromatogr Sci 2018 Nov;56(10):933-940

Systems Biotechnology Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajohesh Blvd, 17th Km of Tehran-Karaj Highway, Tehran, Iran.

High-affinity ligands, such as protein A, can be used to develop biocompatible matrices for antibody purification. In this paper, two methods were used for immobilization of protein A on the chitosan. In the first approach, amino groups of chitosan beads were functionalized with tris(2-aminoethyl)amine to produce amine double-branched moieties, which were subsequently activated with glutaraldehyde. Read More

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http://dx.doi.org/10.1093/chromsci/bmy070DOI Listing
November 2018
2 Reads
1.363 Impact Factor

Analysis of Perfluorinated Compounds in Environmental Water Using Decyl-perfluorinated Magnetic Mesoporous Microspheres as Magnetic Solid-Phase Extraction Materials and Microwave-Assisted Derivatization Followed by Gas Chromatography-mass Spectrometry.

J Chromatogr Sci 2018 Nov;56(10):955-961

Key Laboratory of Applied Organic Chemistry, Higher Institutions of Jiangxi Province, School of Chemstry and Envicronmental Science, Shangrao Normal University, Shangrao, China.

In this work, a new method was developed for perfluorinated compounds (PFCs) analysis in water samples based on decyl-perfluorinated magnetic mesoporous nanocomposites microspheres-assisted extraction and microwave-assisted derivatization followed by gas chromatography-mass spectrometry analysis. The decyl-perfluorinated magnetic mesoporous nanocomposites have several advantages such as fast separation ability, good dispersibility in water sample and high selective preconcentration of PFCs. Various parameters, including eluting solvent and volume, the amounts of absorbents, extraction time and elution time, the microwave-assisted derivatization conditions were optimized. Read More

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http://dx.doi.org/10.1093/chromsci/bmy073DOI Listing
November 2018
1 Read

Separation and Determination of Selected Polyphenols from Medicinal Plants.

J Chromatogr Sci 2019 Jan;57(1):17-26

Department of Inorganic, Analytical Chemistry and Electrochemistry, Silesian University of Technology, 7M. Strzody Str., Gliwice, Poland.

A study of the simultaneous separation and determination of selected polyphenols (rutin, narirutin, naringin, hesperidin, neohesperidin, quercetin, naringenin, kaempferol and hesperetin) with reported effects in the treatment of depression and cardiac and neurodegenerative diseases was performed. An RP-ultrahigh-performance liquid chromatography (UHPLC)-ultraviolet method for analyte separation and determination was successfully developed and validated for a ZORBAX Eclipse Plus C18 RRHD analytical column. Separation was carried out in gradient elution mode with acetonitrile and water modified with 0. Read More

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http://dx.doi.org/10.1093/chromsci/bmy075DOI Listing
January 2019
10 Reads

Speciation of Arsenic in Ground Water Samples by Ion Exchange Chromatography with Improved Sample Preparation Technique.

J Chromatogr Sci 2018 Oct;56(9):853-857

Metrohm India Limited, Chennai, India.

Arsenic exists in the form of various chemical species differing in their physicochemical behavior, toxicity, bioavailability and biotransformation. The determination of arsenic species is an important issue for environmental, clinical and food chemistry. However, differentiation of these species is a quite complex analytical task. Read More

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http://dx.doi.org/10.1093/chromsci/bmy076DOI Listing
October 2018
4 Reads