7,418 results match your criteria Journal of Virological Methods[Journal]


Added value of IgA antibodies against Zika virus non-structural protein 1 in the diagnosis of acute Zika virus infections.

J Virol Methods 2019 Feb 16. Epub 2019 Feb 16.

Institute for Experimental Immunology, Euroimmun AG, Seekamp 31, D-23560, Lübeck, Germany.

Zika virus (ZIKV) is a mosquito-borne flavivirus posing a public health threat due to its association with neurological complications in newborns and adults. In flavivirus-endemic areas, coming mosquito seasons will require the differentiation of primary versus secondary and acute versus past ZIKV/flavivirus infections. This is complicated by two major difficulties: [i] secondary infections often present with low or undetectable titres of specific IgM and early-positive IgG, [ii] previous flavivirus infection(s) or vaccinations cause elevated cross-reactivities. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.005DOI Listing
February 2019

IN SITU hybridization FOR THE LOCALIZATION OF TWO PEPINO MOSAIC VIRUS ISOLATES IN MIXED INFECTIONS.

J Virol Methods 2019 Feb 13. Epub 2019 Feb 13.

Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Departamento de Biología del Estrés y Patología Vegetal, Murcia, Spain. Electronic address:

In situ hybridization (ISH) is an informative and relatively accessible technique for the localization of viral genomes in plant tissue and cells. However, simultaneous visualization of related plant viruses in mixed infections may be limited by the nucleotide similarity in the genomes and the single chromogenic detection over the same sample preparation. To address this issue, we used two Pepino mosaic virus isolates and performed ISH over consecutive serial cross-sections of paraffin-embedded leaf samples of single and mixed infected Nicotiana benthamiana plants. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.003DOI Listing
February 2019

Localized Surface Plasmon Resonance Biosensing of Tomato Yellow Leaf Curl Virus.

J Virol Methods 2019 Feb 13. Epub 2019 Feb 13.

Department of biotechnology and plant breeding, faculty of agriculture, Ferdowsi university of Mashhad, Iran.

Current techniques for plant virus detection, such as RT- PCR and ELISA, require multistep procedures and rely on sophisticated equipment. Due to the global spread of plant viruses, the development of simpler, faster and cheaper assay methods is inevitable. Gold nanoparticles (AuNPs) had raised much interest during recent years due to their novel optical properties or diagnostic purposes. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.004DOI Listing
February 2019
1 Read

Evaluation of a BioRad Avidity assay for identification of recent HIV-1 infections using dried serum or plasma spots.

J Virol Methods 2019 Apr 7;266:114-120. Epub 2019 Feb 7.

Division of HIV and Other Retroviruses, Robert Koch Institute, Berlin, Germany; Charité, Universitätsmedizin, Berlin, Berlin, Germany. Electronic address:

Serological methods to differentiate between recently acquired and established HIV-1 infections are a useful tool in the HIV-surveillance to characterize the epidemic, identify groups at risk and assess HIV-preventive interventions. Therefore, an avidity-based, modified BioRad Genscreen™ HIV-1/2 assay (BRA) was evaluated according to the avidity-based, modified BioRad HIV-1/2 Plus O protocol (BRA). Overall, 692 well defined samples (82. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.002DOI Listing
April 2019
1 Read

Impact of long-term storage of clinical samples collected from 1996 to 2017 on RT-PCR detection of norovirus.

J Virol Methods 2019 Feb 5. Epub 2019 Feb 5.

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, Georgia, 30333, USA. Electronic address:

Noroviruses are recognized as the leading cause of acute gastroenteritis globally. With improved molecular diagnostics developed over the last two decades, archived clinical specimens are increasingly used to investigate the historic prevalence and molecular epidemiology of human norovirus. Yet the impact of long-term storage on viral integrity in clinical specimens has not been evaluated. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.001DOI Listing
February 2019

Multiplex real-time RT-PCR for detection and distinction of Spondweni and Zika virus.

J Virol Methods 2019 Apr 4;266:72-76. Epub 2019 Feb 4.

Centre of Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa; Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa. Electronic address:

Zika (ZIKV) and Spondweni viruses (SPOV) are closely related mosquito borne flaviviruses in the Spondweni serogroup. The co-circulation and similar disease presentation following ZIKV and SPOV infection necessitates the development of a diagnostic tool for their simultaneous detection and distinction. We developed a one-step multiplex real-time RT-PCR (ZIKSPOV) to detect and distinguish between SPOV and ZIKV by utilizing a single primer set combined with virus specific hydrolysis probes. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.011DOI Listing
April 2019
1 Read

Using a new serotype-specific Polymerase Chain Reaction (PCR) and sequencing to differentiate between field and vaccine-derived African Horse Sickness viruses submitted in 2016/2017.

J Virol Methods 2019 Apr 2;266:89-94. Epub 2019 Feb 2.

Agricultural Research Council - Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa. Electronic address:

The outer capsid viral protein 2 (VP2) of African horse sickness virus, encoded by the most variable genome segment 2 (Seg-2), is the primary target for AHSV-specific neutralising antibodies and thus determines the virus serotype. Full length segment 2 sequences from more than 100 AHSVs isolated over the last 80 years were compared and single nucleotide polymorphisms (SNPs) identified between the reference strains and recent field viruses. Regions unique to each individual serotype were identified and primers designed to differentially amplify each of the nine serotypes. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.016DOI Listing
April 2019
3 Reads

The review of differential equation models of HBV infection dynamics.

Authors:
Miaolei Li Jian Zu

J Virol Methods 2019 Apr 1;266:103-113. Epub 2019 Feb 1.

School of Mathematics and Statistics, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China. Electronic address:

Understanding the infection and pathogenesis mechanism of hepatitis B virus (HBV) is very important for the prevention and treatment of hepatitis B. Mathematical models contribute to illuminate the dynamic process of HBV replication in vivo. Therefore, in this paper we review the viral dynamics in HBV infection, which may help us further understand the dynamic mechanism of HBV infection and efficacy of antiviral treatment. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.014DOI Listing
April 2019
1 Read

Evaluation of different genomic regions of Rotavirus A for development of real time PCR.

J Virol Methods 2019 Apr 30;266:65-71. Epub 2019 Jan 30.

Enteric Viruses Group, ICMR-National Institute of Virology, Pune, India. Electronic address:

The nucleotide alignment of all 11 genes of human Rotavirus A (RVA) strains revealed suitability of NSP2, NSP3 and VP6 genes for the development of real time PCR (qRT-PCR). Evaluation of qRT-PCR assays using known rotavirus ELISA positive and negative fecal specimens showed non-overlapping ranges of Mean ±3SD cycle threshold (Ct) values for NSP3 and VP6 based assays. Using serial dilutions of purified RVA, high sensitivity of VP6 qRT-PCR assay (1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183058
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http://dx.doi.org/10.1016/j.jviromet.2019.01.017DOI Listing
April 2019
2 Reads

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.

J Virol Methods 2019 Apr 28;266:58-64. Epub 2019 Jan 28.

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Mixed infections with different pathogens are common in sheep and goats under intensive production conditions. Quick and accurate detection and differentiation of different pathogens is necessary for epidemiological surveillance, disease management and import and export controls. Multiplex TaqMan qPCR protocols were developed and subsequently evaluated as effective tools in simultaneously detecting single and mixed infections in sheep and goats. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.015DOI Listing
April 2019
1.883 Impact Factor

Application of deep sequencing methods for inferring viral population diversity.

J Virol Methods 2019 Apr 25;266:95-102. Epub 2019 Jan 25.

Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan; Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan. Electronic address:

The first deep sequencing method was announced in 2005. Due to an increasing number of sequencing data and a reduction in the costs of each sequencing dataset, this innovative technique was soon applied to genetic investigations of viral genome diversity in various viruses, particularly RNA viruses. These deep sequencing findings documented viral epidemiology and evolution and provided high-resolution data on the genetic changes in viral populations. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.013DOI Listing

Validation of an immunoblot assay employing an objective reading system and used as a confirmatory test in equine infectious anaemia surveillance programs.

J Virol Methods 2019 Apr 23;266:77-88. Epub 2019 Jan 23.

Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Via Appia Nuova 1411, 00178, Rome, Italy. Electronic address:

Equine infectious anaemia (EIA) is a blood borne disease that is listed among the notifiable diseases of the World Organisation for Animal Health (OIE). EIA is also regulated by the OIE for the international trading provisions and is generally subject to control programmes. Since 2011, Italy has been conducting a surveillance plan based on a three-tier diagnostic system, using a serological ELISA as screening test, an agar gel immunodiffusion test (AGIDT) as a confirmatory method, and an immunoblot (IB) as an alternative confirmatory assay for discordant results between the first two tests. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.012DOI Listing
April 2019
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Evaluation of two workflows for whole genome sequencing-based typing of influenza A viruses.

J Virol Methods 2019 Apr 21;266:30-33. Epub 2019 Jan 21.

Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address:

We compared two sample preparation protocols for whole genome sequencing of influenza A viruses. Each protocol was assessed using cDNA quantity and quality and the resulting mean genome coverage after sequencing. Both protocols produced acceptable result for samples with high viral load, whereas one protocol performed slightly better with limited virus count. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.009DOI Listing

Rapid and sensitive detection of goose parvovirus and duck-origin novel goose parvovirus by recombinase polymerase amplification combined with a vertical flow visualization strip.

J Virol Methods 2019 Apr 22;266:34-40. Epub 2019 Jan 22.

Zhongkai University of Agriculture and Engineering, Guangdong, Guangzhou, 510225, China; Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangdong, Guangzhou, 510225, China. Electronic address:

Goose parvovirus (GPV) is one of the most serious viral pathogens in goslings. Recently, a new pathogen to the Chinese mainland-duck-origin novel goose parvovirus (N-GPV)-was found to be 90.8-94. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.010DOI Listing
April 2019
5 Reads

Poliovirus-binding inhibition ELISA based on specific chicken egg yolk antibodies as an alternative to the neutralization test.

J Virol Methods 2019 Apr 17;266:7-10. Epub 2019 Jan 17.

Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (FSBSI "Chumakov FSC R&D IBP RAS"), Moscow, 108819, Russia; Sechenov First Moscow State Medical University, Moscow, 119991, Russia.

The first application results of enzyme immunoassay system (ELISA) - binding inhibition ELISA (BI ELISA) for the detection of antibodies to polioviruses of three types based on the use of specific antibodies from chicken yolk (IgY) are presented. This variant of ELISA is a "surrogate" neutralization test (NT). When comparing the results of the detection of antibodies in 90 sera of children who were vaccinated with oral and inactivated poliovirus vaccines, good correlation (r = 0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183053
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http://dx.doi.org/10.1016/j.jviromet.2019.01.007DOI Listing
April 2019
3 Reads

Critical issues in application of molecular methods to environmental virology.

J Virol Methods 2019 Apr 16;266:11-24. Epub 2019 Jan 16.

Department of Civil & Environmental Engineering & Earth Sciences, University of Notre Dame, USA.

Waterborne diseases have significant public health and socioeconomic implications worldwide. Many viral pathogens are commonly associated with water-related diseases, namely enteric viruses. Also, novel recently discovered human-associated viruses have been shown to be a causative agent of gastroenteritis or other clinical symptoms. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.008DOI Listing
April 2019
2 Reads

Prospective evaluation of diagnostic tools for respiratory viruses in children and adults.

J Virol Methods 2019 Apr 15;266:1-6. Epub 2019 Jan 15.

Department of Microbiology, Laboratoire des Hôpitaux Universitaires Bruxellois, Brussels, Belgium.

Aim: To compare the performances of molecular and non-molecular tests to diagnose respiratory viral infections and to evaluate the pros and contras of each technique.

Methods: Two hundred ninety-nine respiratory samples were prospectively explored using multiplex molecular techniques (FilmArray Respiratory Panel, Clart Pneumovir), immunological techniques (direct fluorescent assay, lateral flow chromatography) and cell cultures.

Findings: Molecular techniques permitted the recovery of up to 50% more respiratory pathogens in comparison to non-molecular methods. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.006DOI Listing

Development of real-time RT-PCR assays for two viruses infecting pome fruit.

J Virol Methods 2019 Apr 14;266:25-29. Epub 2019 Jan 14.

Department of Plant Pathology, Washington State University, Prosser, WA, 99350, United States.

Apple stem grooving virus (ASGV) and Apple green crinkle-associated virus (AGCaV) negatively impact production, maintenance, and distribution of apples and other Malus species world-wide. Due to the increasing diversity of isolates found by high-throughput sequencing, we have developed real-time RT-qPCR assays for these two viruses. Primers and probes were designed against alignments of representative extant sequences from around the world, and reaction conditions optimized for sensitivity and specificity. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.008DOI Listing
April 2019
1 Read

Evaluation of rapid and sensitive DNA extraction methods for detection of cytomegalovirus in dried blood spots.

J Virol Methods 2019 Mar 11;265:117-120. Epub 2019 Jan 11.

Centers for Disease Control and Prevention, 4770 Buford Hwy. NE, Atlanta, GA 30341, USA.

Background: Dried blood spots (DBS), collected universally from newborns in the U.S., could be used as a matrix for the detection of cytomegalovirus (CMV) infection in infants. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.005DOI Listing

Validation of a site-specific recombination cloning technique for the rapid development of a full-length cDNA clone of a virulent field strain of vesicular stomatitis New Jersey virus.

J Virol Methods 2019 Mar 9;265:113-116. Epub 2019 Jan 9.

Foreign Animal Disease Research Unit, USDA/ARS Plum Island Animal Disease Center, PO Box 848, Greenport NY 11944, USA. Electronic address:

This study reports the use of a site-specific recombination cloning technique for rapid development of a full-length cDNA clone that can produce infectious vesicular stomatitis New Jersey virus (VSNJV). The full-length genome of the epidemic VSNJV NJ0612NME6 strain was amplified in four overlapping cDNA fragments which were linked together and cloned into a vector plasmid by site-specific recombination. Furthermore, to derive infectious virus, three supporting plasmid vectors containing either the nucleoprotein (N), phosphoprotein (P) or polymerase (L) genes were constructed using the same cloning methodology. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.003DOI Listing

Application of high-resolution melting curve analysis for identification of Muscovy duck parvovirus and goose parvovirus.

J Virol Methods 2019 Apr 9;266:121-125. Epub 2019 Jan 9.

Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Key Laboratory of Livestock Disease Prevention of Guangdong Province, Guangzhou 510640, China; Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture, Guanzhou, 510640, China. Electronic address:

This study reports the findings of a high-resolution melting (HRM) curve analysis combined with PCR technique (PCR-HRM) to differentiate between Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV). A degenerate primer set was designed based on the VP3 gene of MDPV and GPV. The PCR HRM assay was able to discriminate between MDPVs and GPVs by differences in melting curve shapes and melting temperatures. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183032
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http://dx.doi.org/10.1016/j.jviromet.2018.12.018DOI Listing
April 2019
6 Reads

Evaluation of a rapid isothermal nucleic acid amplification kit, Alere™ i Influenza A&B, for the detection of avian influenza viruses.

J Virol Methods 2019 Mar 8;265:121-125. Epub 2019 Jan 8.

Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Global Station of Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Hokkaido 001-0020, Japan. Electronic address:

Rapid and accurate diagnosis of influenza virus infection is essential for quick responses for both human and animal health. The Alere™ i Influenza A&B is a novel isothermal nucleic acid amplification kit that can detect and differentiate between influenza A and B viruses in human specimens in approximately 15 min. In the present study, the performance of the Alere™ i Influenza A&B kit was evaluated for its ability to detect avian influenza virus in chickens. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.004DOI Listing
March 2019
1 Read

Recovery of recombinant infectious hematopoietic necrosis virus strain Sn1203 using the mammalian cell line BHK-21.

J Virol Methods 2019 Mar 4;265:84-90. Epub 2019 Jan 4.

Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, China. Electronic address:

Reverse genetics systems are powerful tools for understanding the virulence mechanisms and gene functions of negative-sense RNA viruses. The reverse genetics systems commonly used for recombinant infectious hematopoietic necrosis virus (IHNV) are based on vaccinia virus infection. To avoid the potential biological safety risks associated with vaccinia virus, a recombinant IHNV virus strain Sn1203 (rIHNV-Sn1203) was rescued in this study using a mammalian cell line, BHK-21. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.002DOI Listing
March 2019
1 Read

Comparative analysis of the performance of residual host-cell DNA assays for viral vaccines produced in Vero cells.

J Virol Methods 2019 Jan 3. Epub 2019 Jan 3.

Sanofi Pasteur, 1541 avenue Marcel Mérieux, 69280, Marcy l'Etoile, France.

Residual host cell DNA (rcDNA) from continuous cell lines used for manufacturing of biological medicinal products has been considered as safety risk. Historically, several analytical methods have been used for rcDNA quantitation including hybridization assay, Threshold assay and quantitative polymerase chain reaction (qPCR). Sanofi Pasteur has a wealth of experience in the development of methods quantifying rcDNA in vaccines. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183048
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http://dx.doi.org/10.1016/j.jviromet.2019.01.001DOI Listing
January 2019
8 Reads

New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP).

J Virol Methods 2019 Mar 26;265:91-98. Epub 2018 Dec 26.

Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), 9820 Merelbeke, Belgium. Electronic address:

Little cherry virus 1 (LChV-1) belongs to the genus Velarivirus, family Closteroviridae, is an economically important pathogen affecting mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site detection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934173079
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http://dx.doi.org/10.1016/j.jviromet.2018.12.019DOI Listing
March 2019
1 Read

Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus.

J Virol Methods 2019 Mar 26;265:66-70. Epub 2018 Dec 26.

Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. Electronic address:

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was associated with severe diarrhea disease in pigs. SADS-CoV was first detected and identified as the causative agent of a devastating swine disease outbreak in southern China in 2017. Routine monitoring and early detection of the source of infection is therefore integral to the prevention and control of SADS-CoV infection. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.010DOI Listing
March 2019
2 Reads

Genetic identification of two Acipenser iridovirus-European variants using high-resolution melting analysis.

J Virol Methods 2019 Mar 23;265:105-112. Epub 2018 Dec 23.

Laboratoire de Ploufragan-Plouzané, ANSES, Technopole Brest Iroise, 29280 Plouzané, France. Electronic address:

Acipenser iridovirus-European (AcIV-E) is an important pathogen of sturgeons. Two variants differing by single-nucleotide polymorphisms (SNP) in the Major Capsid Protein gene have been described, but without any indication as to their prevalence in farms. To facilitate epidemiological studies, we developed a high-resolution melting (HRM) assay to distinguish between two alleles (var1 and var2) differing by five point substitutions. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.006DOI Listing

Enhanced in vitro virus expression using 3-dimensional cell culture spheroids for infection.

J Virol Methods 2019 Mar 21;265:99-104. Epub 2018 Dec 21.

Virology Unit, Pisa University Hospital, Pisa, Italy; Retrovirus Center and Virology Section, Department of Translational Medicine, University of Pisa, Via del Brennero 2, I-56127 Pisa, Italy.

The way viruses interact with cultured cells and their surrounding environment is still a matter of debate. From a technical point of view, 2D cell cultures only partially exhibit the morpho-molecular pattern required for viral tropism, not reflecting the complexity of the microenvironment in vivo. Therefore, 3D cell cultures are envisioned as an alternative approach to study viral replication possibly closer to in vivo conditions than 2D, representing the link between traditional cell culture and in vivo models. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.017DOI Listing

Evaluation of first rapid diagnostic kit for Anti-Crimean-Congo Hemorrhagic Fever virus IgM antibody using clinical samples from Iran.

J Virol Methods 2019 Mar 21;265:49-52. Epub 2018 Dec 21.

Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran; Research Center for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran. Electronic address:

Crimean-Congo Hemorrhagic Fever (CCHF) is a potentially fatal tick-borne viral disease, which is in the WHO list for emerging infections likely to cause major epidemics in the near future. Early diagnosis of CCHF is very important for both patient treatment and infection control. An efficient CCHF rapid test therefore is of great significance. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.015DOI Listing
March 2019
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Production of a polyclonal antiserum against recombinant nucleocapsid protein and its application for the detection of fig mosaic virus.

J Virol Methods 2019 Mar 21;265:22-25. Epub 2018 Dec 21.

Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano, BA, Italy. Electronic address:

Mosaic disease (MD), caused by Fig mosaic emaravirus (FMV), is the most important and devastating virus disease of fig trees worldwide. The detection of FMV in infected plants is possible only through the use of molecular techniques, i.e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183036
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http://dx.doi.org/10.1016/j.jviromet.2018.12.016DOI Listing
March 2019
1 Read

Development of an avian avulavirus 1 (AAvV-1) L-gene real-time RT-PCR assay using minor groove binding probes for application as a routine diagnostic tool.

J Virol Methods 2019 Mar 21;265:9-14. Epub 2018 Dec 21.

Animal and Plant Health Agency (APHA), Woodham Lane, Addlestone, Surrey, KT15 3NB, United Kingdom.

Newcastle disease is a devastating disease of poultry caused by Newcastle disease virus (NDV), a virulent form of avian avulavirus 1 (AAvV-1). A rapid, sensitive and specific means for the detection of NDV is fundamental for the control of this notifiable transboundary virus. Although several real-time RT-PCR assays exist for the detection of AAvV-1, diagnostic sensitivity and specificities can be sub-optimal. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.001DOI Listing

A novel real-time PCR assay panel for detection of common respiratory pathogens in a convenient, strip-tube array format.

J Virol Methods 2019 Mar 19;265:42-48. Epub 2018 Dec 19.

Children's and Women's Health Centre of BC, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.

Commercial multiplex assays, built on different chemistries and platforms are widely available for simultaneous detection of pathogens that cause respiratory infections. However, these tests are often difficult to implement in a resource limited setting because of high cost. In this study, we developed and validated a method for simultaneous testing of common respiratory pathogens (Respanel) by real-time PCR in a convenient, strip-tube array format. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.013DOI Listing

A plant intron enhances the performance of an infectious clone in planta.

J Virol Methods 2019 Mar 19;265:26-34. Epub 2018 Dec 19.

Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:

Although infectious clones are fundamental tools in virology and plant pathology, their efficacy is often reduced by the instability of viral sequences in Escherichia coli. In this study, we constructed an infectious clone of PepMoV (pPepMoV) in a bacterial binary vector (pSNU1); the clone induces symptoms of PepMoV in agroinfiltrated plants. During its modification and maintenance in E. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.012DOI Listing
March 2019
1 Read

Duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemum.

J Virol Methods 2019 Apr 19;266:41-48. Epub 2018 Dec 19.

Departamento de Investigación y desarrollo, Universidad Católica de Oriente, Sector 3, cra. 46 No. 40B 50, Rionegro, Colombia.

A novel duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemums was developed. Previous reported primers for amplification of TSWV and CSVd were used and a novel pair of primers for CSVd was designed to improve duplex amplification compatibility. Sensitivity and efficiency of the previous reported and novel primers for CSVd were assessed. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.007DOI Listing

In-field capable loop-mediated isothermal amplification detection of Turnip yellows virus in plants and its principal aphid vector Myzus persicae.

J Virol Methods 2019 Mar 19;265:15-21. Epub 2018 Dec 19.

Sustainability and Biosecurity, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, Kensington, Western Australia, 6151, Australia.

Widespread Turnip yellows virus (TuYV) infection causes severe seed yield and quality losses in rapeseed (Brassica napus) crops grown in broadacre agricultural systems worldwide. Current TuYV detection protocols are expensive and time consuming, and can have poor specificity and sensitivity. Typically, they are used as a diagnostic tool to test already symptomatic plants, limiting their practical value to reactive disease management. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.014DOI Listing

Serological diagnosis of equine infectious anemia in horses, donkeys and mules using an ELISA with a gp45 synthetic peptide as antigen.

J Virol Methods 2019 Apr 19;266:49-57. Epub 2018 Dec 19.

Laboratório de Retroviroses da Escola de Veterinária da Universidade Federal de Minas Gerais, Brazil. Electronic address:

Equine infectious anemia (EIA) is a disease caused by a Lentivirus that is currently controlled exclusively by identification of seropositive animals. In most countries, including Brazil, the official diagnostic test for EIA is the agar gel immunodiffusion test (AGID). Although this assay has a high specificity it can produce false negative reactions or equivocal results due to weak precipitation lines, especially in samples from donkeys, mules or newly infected equids. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.009DOI Listing

A multiplex reverse transcription PCR assay for simultaneous detection of six main RNA viruses in tomato plants.

J Virol Methods 2019 Mar 18;265:53-58. Epub 2018 Dec 18.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address:

Tomato virus diseases occur all around the world, causing serious yield losses. To detect these viruses quickly and provide a basis for disease control, a multiplex reverse transcription polymerase chain reaction system was established for simultaneous detection of Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), Tomato chlorosis virus (ToCV), Potato virus Y (PVY) and Potato virus X (PVX) in tomato plants, with 6 pairs of specific primers being designed based on the coat protein (CP) genes of these viruses. Transcriptional elongation factor-1α (EF-1α) from tomato was added to the multiplex RT-PCR reaction system to prevent false negatives. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934173056
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http://dx.doi.org/10.1016/j.jviromet.2018.12.011DOI Listing
March 2019
3 Reads

Recombinant DENV 2 NS5: An effective antigen for diagnosis of DENV infection.

J Virol Methods 2019 Mar 16;265:35-41. Epub 2018 Dec 16.

Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, China.

Dengue fever is a mosquito-borne viral disease with dramatically increasing morbidity rate worldwide in decades. Since there is no specific treatment to date, early diagnosis is important for providing proper timely medical care to minimize mortality, and for the prompt initiation of public health control measures. NS5 is a potential biomarker for dengue virus infection due to its highly conserved and immunogenic properties. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183006
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http://dx.doi.org/10.1016/j.jviromet.2018.12.005DOI Listing
March 2019
2 Reads
1.883 Impact Factor

Development of a graft inoculation method and a real-time RT-PCR assay for monitoring Tomato chlorosis virus infection in tomato.

J Virol Methods 2019 Mar 14;265:1-8. Epub 2018 Dec 14.

Applied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Agricultural Biotechnology, 32260, Isparta, Turkey.

A graft inoculation method coupled with RT-qPCR was developed for monitoring ToCV infection in tomato plants. Ten seed-grown tomato seedlings were graft inoculated with phloem tissue-containing stem segments from a ToCV-infected tomato plants. Another group of tomato seedling were grafted with similar stem segments from a healthy tomato plant as mock inoculated control. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.004DOI Listing
March 2019
1 Read

Diagnostic protocols for the detection of Acheta domesticus densovirus (AdDV) in cricket frass.

J Virol Methods 2019 Feb 5;264:61-64. Epub 2018 Dec 5.

Department of Ecology, Swedish University of Agricultural Sciences, Uppsala 750 07, Sweden.

The European house cricket (Acheta domesticus) is a species of interest for the emerging insect-as-food industry. Acheta domesticus densovirus (AdDV) is a member of the Parvoviridae virus family which infects A. domesticus, causing widespread mortality and even extinction of local cricket populations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183045
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http://dx.doi.org/10.1016/j.jviromet.2018.12.003DOI Listing
February 2019
10 Reads

Standardized focus assay protocol for biosafety level four viruses.

J Virol Methods 2019 Feb 1;264:51-54. Epub 2018 Dec 1.

Spiez Laboratory, Federal Office for Civil Protection, Austrasse, 3700, Spiez, Switzerland. Electronic address:

Working in accordance with biosafety level four practices is highly complex and time-consuming. Therefore, the respective laboratory protocols should be as uniform as possible, simple to perform and straightforward in readout. Here we describe the successful application of a standardized 24-well plate focus assay protocol for the titration of Zaire ebolavirus (two isolates), Marburg virus (three isolates), Lassa virus (two isolates), Crimean Congo hemorrhagic fever virus (one isolate), and tick-borne encephalitis virus (two isolates). Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.12.002DOI Listing
February 2019
1 Read

Corrigendum to "Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers" [J. Virol. Methods 259 (2018) 122-128].

J Virol Methods 2019 Jan;263:120

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China. Electronic address:

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http://dx.doi.org/10.1016/j.jviromet.2018.11.010DOI Listing
January 2019

A high-throughput inhibition assay to study MERS-CoV antibody interactions using image cytometry.

J Virol Methods 2019 Mar 20;265:77-83. Epub 2018 Nov 20.

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address:

The emergence of new pathogens, such as Middle East respiratory syndrome coronavirus (MERS-CoV), poses serious challenges to global public health and highlights the urgent need for methods to rapidly identify and characterize potential therapeutic or prevention options, such as neutralizing antibodies. Spike (S) proteins are present on the surface of MERS-CoV virions and mediate viral entry. S is the primary target for MERS-CoV vaccine and antibody development, and it has become increasingly important to understand MERS-CoV antibody binding specificity and function. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183031
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http://dx.doi.org/10.1016/j.jviromet.2018.11.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357230PMC
March 2019
19 Reads
1.883 Impact Factor

Human papillomavirus detection in urine: Effect of a first-void urine collection device and timing of collection.

J Virol Methods 2019 Feb 16;264:23-30. Epub 2018 Nov 16.

Centre for the Evaluation of Vaccination (CEV), Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Belgium.

Great interest has been directed towards the use of first-void (FV) urine as a liquid biopsy for high-risk HPV DNA testing. The aim of this study was to investigate the potential effect of a first generation FV urine collection device on the detection of HPV DNA and to assess if the concentration of HPV DNA varies between FV urine collected in the morning and those collected later during the day. In this prospective cohort study, 33 self-reported HPV-positive women participated. Read More

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http://dx.doi.org/10.1016/j.jviromet.2018.11.008DOI Listing
February 2019
14 Reads

Verification of the rabies virus glycoprotein lower limit of immunogenicity by serological assay.

J Virol Methods 2019 Feb 15;264:31-37. Epub 2018 Nov 15.

Instituto Nacional de Controle de Qualidade em Saúde (INCQS), Fundação Oswaldo Cruz (FIOCRUZ), Av. Brasil, 4365, Manguinhos, CEP: 21040-900, Rio de Janeiro, RJ, Brazil; Centro Brasileiro de Validação de Métodos Alternativos (BraCVAM), INCQS, FIOCRUZ, Brazil. Electronic address:

Rabies lethality is close to 100% and annually 15 million people receive post-exposure prophylaxis. Testing for vaccines against this zoonosis should ensure its quality. A standardized test by the National Institutes of Health (NIH) test, based on mice immunization and challenge, has been used to determine the potency of vaccine lots. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183038
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http://dx.doi.org/10.1016/j.jviromet.2018.11.005DOI Listing
February 2019
11 Reads

Deep sequencing prompts the modification of a real-time RT-PCR for the serotype-specific detection of polioviruses.

J Virol Methods 2019 Feb 14;264:38-43. Epub 2018 Nov 14.

Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA, United States; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States. Electronic address:

Polioviruses are members of the Enterovirus C species and asymptomatic fecal shedding allows for their transmission and persistence in a community, as well as the emergence of vaccine-derived polioviruses. Using three serotype-specific real-time RT-PCR (rRT-PCR) assays, the shedding and circulation of oral poliovirus vaccine (OPV) strains was previously investigated in a prospective cohort of Mexican children, their contacts, and nearby sewage. Subsequently, a deep sequencing approach targeting the P1 genomic region was applied to characterize OPV strains previously detected by rRT-PCR. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183036
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http://dx.doi.org/10.1016/j.jviromet.2018.11.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320388PMC
February 2019
5 Reads

Development of a loop-mediated isothermal amplification assay for the detection and quantification of epizootic epitheliotropic disease virus (salmonid herpesvirus-3).

J Virol Methods 2019 Feb 13;264:44-50. Epub 2018 Nov 13.

Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824, USA; Comparative Medicine and Integrative Biology, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA; Department of Fisheries and Wildlife, College of Agriculture and Natural Resources,Michigan State University, East Lansing, MI 48824, USA. Electronic address:

Epizootic Epitheliotropic Disease Virus (EEDV; Salmonid Herpesvirus-3) causes a serious disease hatchery-reared lake trout (Salvelinus namaycush), threatening restoration efforts of this species in North America. The current inability to replicate EEDV in vitro necessitates the search for a reproducible, sensitive, and specific assay that allows for its detection and quantitation in a time- and cost-effective manner. Herein, we describe a loop-mediated isothermal amplification (LAMP) assay that was developed for the quantitative detection of EEDV in infected fish tissues. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183021
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http://dx.doi.org/10.1016/j.jviromet.2018.11.006DOI Listing
February 2019
12 Reads

Development of an in-situ hybridization assay using riboprobes for detection of viral haemorrhagic septicemia virus (VHSV) mRNAs in a cell culture model.

J Virol Methods 2019 Feb 8;264:1-10. Epub 2018 Nov 8.

Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University, Yeosu, 59626, Republic of Korea. Electronic address:

An in situ hybridization (RNA-ISH) assay has been developed and optimized to detect viral haemorrhagic septicemia virus (VHSV), an OIE listed piscine rhabdovirus, in infected fish cells using fathead minnow (FHM) as a model cell line. Two antisense riboprobes (RNA probes) targeting viral transcripts from a fragment of nucleoprotein (N) and glycoprotein (G) genes were generated by reverse transcription polymerase chain reaction (RT-PCR) using VHSV specific primers followed by a transcription reaction in the presence of digoxigenin dUTP. The synthesized RNA probes were able to detect viral mRNAs in formalin fixed VHSV infected FHM cells at different time points post inoculation (pi). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183044
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http://dx.doi.org/10.1016/j.jviromet.2018.11.003DOI Listing
February 2019
6 Reads
1.883 Impact Factor

Strip-dried blood sampling: applicability for bovine leukemia virus detection with ELISA and real-time PCR.

J Virol Methods 2019 Jan 6;263:101-104. Epub 2018 Nov 6.

Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991, Moscow, Russia; National University of Science and Technology "MISiS", 119049, Moscow, Russia.

We recently proposed a new so-called strip-dried format aimed for convenient use of dried biomaterial in diagnostic purposes. In this work, 334 blood samples obtained in strip-dried form were used for bovine leucosis analysis with ELISA and real-time PCR methods. High percentage of seropositive animals (18. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183029
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http://dx.doi.org/10.1016/j.jviromet.2018.11.004DOI Listing
January 2019
15 Reads

Characterization of a quasi-enveloped, fast replicating hepevirus from fish and its use as hepatitis E virus surrogate.

J Virol Methods 2019 Jan 3;263:111-119. Epub 2018 Nov 3.

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. Electronic address:

Hepatitis E virus (HEV) is an emerging concern for the safety of plasma-derived medicinal products. The lack of an efficient cell culture system hampers the studies on HEV biology as well as validation studies to test the capacity of virus reduction steps to clear HEV. Hence, a surrogate hepevirus that can efficiently replicate in cell culture is needed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183042
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http://dx.doi.org/10.1016/j.jviromet.2018.11.002DOI Listing
January 2019
9 Reads
1.883 Impact Factor