7,454 results match your criteria Journal of Virological Methods[Journal]


Comparison of multiplex-serology and ELISA based methods in detecting HPV16 L1 antibody responses in paired saliva and serum samples of healthy men.

J Virol Methods 2019 Apr 17. Epub 2019 Apr 17.

Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland; Department of Pathology, Turku University Hospital, Kiinamyllynkatu 4-8, 20521 Turku, Finland.

Human papilloma viruses (HPV) are a common cause of transient infections on mucosal surfaces, also in the oral cavity. Some infections remain persistent and can, especially with high risk HPV genotypes, lead to malignancies in the oral-oropharyngeal area. Our understanding of the natural course of oral HPV infections is limited, and the local host responses are poorly known. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.007DOI Listing

Comparison of nucleic acid extraction methods for next-generation sequencing of avian influenza A virus from ferret respiratory samples.

J Virol Methods 2019 Apr 17. Epub 2019 Apr 17.

Influenza Division, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA, 30329, USA. Electronic address:

Influenza A virus is a negative-sense RNA virus with a segmented genome consisting of eight RNA segments. Avian influenza A virus (AIV) primarily infects avian hosts and sporadically infects mammals, which can lead to adaptation to new species. Next-generation sequencing (NGS) of emerging AIV genomes extracted from respiratory samples collected on sequential days from animal models and clinical patients enables analysis of the emergence of evolutionary variants within the virus population over time. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.014DOI Listing

Screening of novel drugs for inhibiting hepatitis E virus replication.

J Virol Methods 2019 Apr 17. Epub 2019 Apr 17.

Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-Shi, Tochigi 329-0498, Japan. Electronic address:

Hepatitis E, which is caused by hepatitis E virus (HEV), is generally a self-limiting, acute, and rarely fatal disease. It is sometimes fulminant and lethal, especially during pregnancy. Indeed, it occasionally takes a chronic course in immunocompromised individuals. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183029
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http://dx.doi.org/10.1016/j.jviromet.2019.04.017DOI Listing
April 2019
1 Read

Increased HPV detection by the use of a pre-heating step on vaginal self-samples analysed by Aptima HPV assay.

J Virol Methods 2019 Apr 16. Epub 2019 Apr 16.

Department of Medical Microbiology, Laboratory Medicine Region Skåne, Lund University, Lund, Sweden. Electronic address:

Background: We recently reported a sensitivity of 85.5% to detect high-grade squamous intraepithelial lesions (HSIL)/adenocarcinoma in situ (AIS)/cancer by the use of self-collected vaginal samples analysed by the Aptima mRNA HPV assay (AHPV).

Objectives: To increase detection of HPV among self-samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934193011
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http://dx.doi.org/10.1016/j.jviromet.2019.04.015DOI Listing
April 2019
1 Read

Development of a Bead-based Immunoassay Using Virus-like Particles for Detection of Alphaviral Humoral Response.

J Virol Methods 2019 Apr 15. Epub 2019 Apr 15.

Diagnostics Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA. Electronic address:

There is a pressing need for sustainable and sensitive immunodiagnostics for use in public health efforts to understand and combat the threat of endemic and emerging infectious diseases. In this proof-of-concept work, we describe an immunodiagnostic approach based on the utilization of virus-like particles (VLPs) in a magnetic bead-based platform for multiplexed detection of antiviral humoral response. A retroviral-based VLP, that presents Venezuelan equine encephalitis virus E1/E2 glycoprotein antigen on its surface, was synthesized and coupled to magnetic beads to create VLP-conjugated microspheres (VCMs). Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.013DOI Listing

Comparison of urine, self-collected vaginal swab, and cervical swab samples for detecting human papillomavirus (HPV) with Roche Cobas HPV, Anyplex II HPV, and RealTime HR-S HPV assay.

J Virol Methods 2019 Apr 15;269:77-82. Epub 2019 Apr 15.

Department of Obstetrics and Gynecology, Korea University Guro Hospital, College of Medicine, Korea University, Seoul, Republic of Korea. Electronic address:

Background: Human papillomavirus (HPV) is well established as the main cause of cervical cancer. Non-invasive self-collected urine and vaginal sampling have the potential advantage of increasing patient compliance with cervical cancer screening.

Methods: Self-collected vaginal and urine samples and clinician-collected cervical samples were collected from 101 patients, including 84 patients with high grade squamous intraepithelial lesion and 17 patients with benign ovarian disease. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.012DOI Listing
April 2019
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Development of an RT-LAMP assay for the detection of Lassa viruses in southeast and south-central Nigeria.

J Virol Methods 2019 Apr 8;269:30-37. Epub 2019 Apr 8.

Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan; Graduate School of Biomedical Sciences and Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan; National Research Center for the Control and Prevention of Infectious Diseases (CCPID), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. Electronic address:

Lassa virus (LASV) causes Lassa fever (LF), a viral hemorrhagic fever endemic in West Africa. LASV strains are clustered into six lineages according to their geographic location. To confirm a diagnosis of LF, a laboratory test is required. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.010DOI Listing
April 2019
1 Read

Determination of the optimal method for the concentration and purification of 146S particles for foot-and-mouth disease vaccine production.

J Virol Methods 2019 Apr 8;269:26-29. Epub 2019 Apr 8.

Animal and Plant Quarantine Agency, Gimcheon, Gyeonsangbuk-do, 39660, Republic of Korea. Electronic address:

After the severe outbreak of foot-and-mouth disease (FMD) in South Korea in 2010, the Korean government implemented a vaccination policy and set out to develop an FMD vaccine using a local FMD virus (FMDV) strain. As a part of the basic research for domestic FMD vaccine development, three methods commonly used for the concentration and purification of FMDV to produce FMD vaccine antigens were compared. Among common concentration methods, including polyethylene glycol (PEG) precipitation, ammonium sulfate precipitation, and ultrafiltration, the most effective method both for concentrating 146S particles and eliminating non-structural proteins (NSPs) was found to be PEG precipitation. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.009DOI Listing

A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya.

J Virol Methods 2019 Apr 8;269:70-76. Epub 2019 Apr 8.

Arthropod-Borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS, USA.

Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.011DOI Listing
April 2019
1 Read

Development of a triplex real-time RT-PCR assay for detection and differentiation of three US genotypes of porcine hemagglutinating encephalomyelitis virus.

J Virol Methods 2019 Apr 5;269:13-17. Epub 2019 Apr 5.

Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Rd, Columbus, OH, 43210, USA. Electronic address:

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a single-stranded, positive-sense RNA virus. PHEV mainly causes two types of clinical manifestations representing vomiting and wasting and encephalomyelitis in piglets. However, our recent findings provide strong evidence that PHEV can also cause respiratory disease in older pigs. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.008DOI Listing
April 2019
1 Read

One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.

J Virol Methods 2019 Apr 5;269:43-48. Epub 2019 Apr 5.

Embrapa Suínos e Aves, BR-153, Km 110, Distrito de Tamanduá, Concórdia, CEP 89715-899, Santa Catarina, Brazil. Electronic address:

Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based - one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV subtypes circulating in pigs in Brazil. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.005DOI Listing
April 2019
1 Read

Development of a cucumber green mottle mosaic virus-based expression vector for the production in cucumber of neutralizing epitopes against a devastating animal virus.

J Virol Methods 2019 Apr 5;269:18-25. Epub 2019 Apr 5.

London Research and Development Centre, Agriculture and Agri-Food Canada, London, Ontario, N5V 4T3, Canada; Department of Biology, Western University, London, Ontario, N6A 5B7, Canada. Electronic address:

Virus-based expression systems have been widely exploited for the production of recombinant proteins in plants during the last thirty years. Advances in technology have boosted scale-up manufacturing of plant-made pharmaceuticals to high levels, via the complementation of transient expression and viral vectors. This combination allows proteins of interest to be produced in plants within a matter of days and thus, is well suited for the development of plant-made vaccines or therapeutics against emerging infectious diseases and potential bioterrorism agents. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.006DOI Listing

Establishment of an immunofluorescence assay to detect IgM antibodies to Nipah virus using HeLa cells expressing recombinant nucleoprotein.

J Virol Methods 2019 Apr 4. Epub 2019 Apr 4.

Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.

A novel indirect fluorescent antibody test (IFAT) for detection of IgM against Nipah virus (NiV) was developed using HeLa 229 cells expressing recombinant NiV nucleocapsid protein (NiV-N). The NiV IFAT was evaluated using three panels of sera: a) experimentally produced sera from NiV-N-immunized/pre-immunized macaques, b) post-infection human sera associated with a Nipah disease outbreak in the Philippines in 2014, and c) human sera from a non-exposed Malaysian population. Immunized macaque sera showed a characteristic granular staining pattern of the NiV-N expressed antigen in HeLa 229 cells, which was readily distinguished from negative-binding results of the pre-immunized macaque sera. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.009DOI Listing
April 2019
1 Read
1.883 Impact Factor

Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.

J Virol Methods 2019 Apr 3. Epub 2019 Apr 3.

Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, Oslo, Norway. Electronic address:

Detection of viruses on berries is a challenging task, often hampered by the presence of RT-qPCR inhibiting substances from berry juice. A direct extraction method for virus detection (murine norovirus and GA phage) on frozen raspberries was previously published. We expanded (different types of berries and viruses) and improved the method using MobiSpin S400 columns that filter nucleic acids based on size-exclusion chromatography. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.004DOI Listing

Molecular typing of Bluetongue virus using the nCounter analysis system platform.

J Virol Methods 2019 Apr 2;269:64-69. Epub 2019 Apr 2.

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy. Electronic address:

Bluetongue virus (BTV) is a segmented double-stranded RNA virus, existing in multiple serotypes, belonging to the genus Orbivirus of the family Reoviridae. BTV causes Bluetongue (BT), a major OIE-listed disease of ruminants. Identification of BTV serotype is accomplished using multiple typing assays and tends to be executed based on the known epidemiological situation within a given country. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.002DOI Listing

Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV).

J Virol Methods 2019 Apr 3;269:55-63. Epub 2019 Apr 3.

ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, 462022, Madhya Pradesh, India.

The aim of this study was to develop an improved border disease virus (BDV) specific real time RT-PCR and to evaluate its performance on manually plucked hairs from sheep persistently infected with BDV that may act as a non-invasive alternate sample. The BDV real time RT-PCR assay reported here showed a high analytical sensitivity (10 TCID50/ml), specificity (no reactivity with BVDV-1, BVDV-2, HoBi-like pestivirus and CSFV) and reproducibility. When the assay was validated on 210 samples from BDV-infected and uninfected sheep, it showed a 100% diagnostic sensitivity and specificity with virus isolation. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.04.003DOI Listing
April 2019
1.883 Impact Factor

A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine.

J Virol Methods 2019 Apr 2;269:38-42. Epub 2019 Apr 2.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis coronavirus (TGEV) were selected based on alignments of genomic sequences and then specific primers were designed. The mRT-PCR assay was developed and evaluated for its specificity and sensitivity. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934193002
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http://dx.doi.org/10.1016/j.jviromet.2019.04.001DOI Listing
April 2019
5 Reads

Clinical evaluation of multiplex RT-PCR assays for the detection of influenza A/B and respiratory syncytial virus using a high throughput system.

J Virol Methods 2019 Apr 1;269:49-54. Epub 2019 Apr 1.

University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.

Background: Lower respiratory tract infections are a major threat to public health systems worldwide, with RSV and influenza being the main agents causing hospitalization. In outbreak situations, high-volume respiratory testing is needed. In this study, we evaluated the analytical and clinical performance of a pre-designed primer/probe set for the simultaneous multiplex detection of both viruses on a high-throughput platform, the cobas 6800, using the "open channel" of the system for integration of lab-developed assays for the detection of influenza and RSV. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.015DOI Listing
April 2019
3 Reads

Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus.

J Virol Methods 2019 Jun 28;268:53-55. Epub 2019 Mar 28.

Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.

Although certain mosquito-borne virus, such as Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Yellow fever virus (YFV) and West Nile virus (WNV), are an important public health concern in those countries where transmitter mosquitoes are endemic, several cases of travelers from those endemic countries have been recently reported in Europe. Thus, early diagnosis of these viruses is essential for patient management and adoption of preventive measures. An assay for the simultaneous detection of DENV, CHIKV, ZIKV, YFV and WNV based on a multiplex real-time (RT)-PCR and its usefulness for diagnosis in infection screenings and surveillance of arbovirus in non-endemic countries are described. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.014DOI Listing
June 2019
2 Reads

Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia.

J Virol Methods 2019 Mar 22;269:1-6. Epub 2019 Mar 22.

Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. Electronic address:

Background: The role of respiratory viruses as the major cause of acute lower respiratory tract infections (ALRTIs) in children is becoming increasingly evident due to the use of sensitive molecular detection methods. The aim of this study was to use conventional and molecular detection methods to assess the epidemiology of respiratory viral infections in children less than five years of age that were hospitalized with ALRTIs.

Methods: The cross-sectional study was designed to investigate the occurrence of respiratory viruses including respiratory syncytisl virus (RSV), human metapneumovirus (HMPV), influenza virus A and B (IFV-A and B), parainfluenzavirus 1, 2, 3 and 4 (PIV 1, 2, 3 and 4), human rhinoviruses (HRV), human enterovirus (HEV), human coronaviruses (HCoV) 229E and OC43, human bocavirus (HBoV) and human adenovirus (HAdV) in hospitalized children with ALRTIs, at Hospital Serdang, Malaysia, from June 16 to December 21, 2009. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.013DOI Listing
March 2019
6 Reads
1.883 Impact Factor

Development and evaluation of an indirect ELISA based on recombinant nonstructural protein 3A to detect antibodies to duck hepatitis A virus type 1.

J Virol Methods 2019 Jun 21;268:56-61. Epub 2019 Mar 21.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China. Electronic address:

To develop an indirect enzyme-linked immunosorbent assay(I-ELISA) method based on 3A protein of duck hepatitis A virus type 1(DHAV-1) for detection of DHAV-1 antibody, the recombinant protein 3A of DHAV-1 was expressed in E.coli and detected by Western blotting with DHAV-1 infected duck serum. A 3A-ELISA method using the expressed 3A protein as coating antigen for the detection of antibodies to DHAV-1 was developed. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.012DOI Listing
June 2019
2 Reads
1.883 Impact Factor

A multiplex real-time PCR assay for the detection and differentiation of the newly emerged porcine circovirus type 3 and continuously evolving type 2 strains in the United States.

J Virol Methods 2019 Mar 20;269:7-12. Epub 2019 Mar 20.

Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS 66506, United States; Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, United States. Electronic address:

A multiplex quantitative real-time polymerase chain reaction (mqPCR) assay was developed and validated for the detection and differentiation of porcine circovirus type 3 (PCV3) and type 2 (PCV2) strains. The assay coverage was 97.9% (184/188) for PCV3 and 99. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.011DOI Listing

Performance evaluation of gastrointestinal viral ELIte panel multiplex RT-PCR assay for the diagnosis of rotavirus, adenovirus and astrovirus infection.

J Virol Methods 2019 Jun 19;268:48-52. Epub 2019 Mar 19.

Dpt di Promozione della Salute, Materno infantile, Medicina Interna e Specialistica di eccellenza "G.D'Alessandro", Università di Palermo, Italy.

Rotavirus, adenovirus, norovirus and astrovirus are considered to be among the major causes of sporadic cases and outbreaks of acute gastroenteritis globally. Rapid and accurate identification of enteric viruses is still a challenge for the clinical laboratory. Recently, several molecular platforms for the detection of viral enteric pathogens have become available. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.010DOI Listing
June 2019
2 Reads

A validated semi-nested PCR for rapid detection of scale drop disease virus (SDDV) in Asian sea bass (Lates calcarifer).

J Virol Methods 2019 Jun 18;268:37-41. Epub 2019 Mar 18.

Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand. Electronic address:

Scale drop diseases virus (SDDV), a newly characterized virus of farmed Asian sea bass (Lates calcarifer), has been reported in several countries in Southeast Asia. However, no fully validated detection method is publicly available for disease diagnosis and surveillance. Here, we described a newly developed semi-nested PCR (snPCR) method for detection of the virus from field samples. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.007DOI Listing
June 2019
1 Read
1.883 Impact Factor

Construction of replication-competent oncolytic retroviral vectors expressing R peptide-truncated 10A1 envelope glycoprotein.

J Virol Methods 2019 Jun 18;268:32-36. Epub 2019 Mar 18.

Department of Microbiology, Dankook University, Cheonan, 330-714, Republic of Korea. Electronic address:

Replication-deficient retroviral (RDR) vectors have been generally used for gene therapy, but clinically beneficial transduction efficiency is difficult to achieve with these vectors. In recent times, attention has been focused on the use of murine leukemia virus (MLV)-based replication-competent retroviral (RCR) vectors. RCR vectors have been shown to achieve efficient tumor reduction in a wide variety of cancer models. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.008DOI Listing

Quantification of a legume begomovirus to evaluate soybean genotypes for resistance to yellow mosaic disease.

J Virol Methods 2019 Jun 16;268:24-31. Epub 2019 Mar 16.

School of Life Sciences, Devi Ahilya Vishwavidhyalaya, Indore, Madhya Pradesh, India.

Mungbean yellow mosaic India virus (MYMIV) infecting soybean and other legumes causes yellow mosaic disease (YMD). Evaluation of soybean genotypes for YMD resistance involves field screening at disease hot spots or in a protected environment using infectious clones or viruliferous whiteflies as sources of virus inocula. Development of efficient virus inoculation and quantification protocols to screen soybean genetic stocks against YMD is imperative for breeding resistant varieties. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.002DOI Listing
June 2019
3 Reads
1.883 Impact Factor

Performance of Bio-Rad HIV-1/2 Confirmatory Assay in HIV-1, HIV-2 and HIV-1/2 dually reactive patients - comparison with INNO-LIA and immunocomb discriminatory assays.

J Virol Methods 2019 Jun 11;268:42-47. Epub 2019 Mar 11.

Department of Translational Medicine, Infectious Diseases Unit, Lund University, Malmö, Sweden.

Background: Being able to discriminate between HIV-1, HIV-2 and HIV-1/2 dual infection is imperative for the appropriate selection of antiretroviral therapy (ART) in regions with high HIV-2 endemicity.

Objectives: To evaluate Bio-Rad Geenius HIV-1/2 Confirmatory Assay against INNO-LIA HIV 1/2 Score and ImmunoComb HIV 1/2 BiSpot with an emphasis towards ability to discriminate between HIV-1, HIV-2 and HIV-1/2 dual infection.

Material And Methods: 131 samples from ART naïve HIV infected patients in Guinea-Bissau were selected retrospectively and tested with Geenius, INNO-LIA and Immunocomb. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.005DOI Listing
June 2019
1 Read

Minor groove binder modification of widely used TaqMan hydrolysis probe for detection of dengue virus reduces risk of false-negative real-time PCR results for serotype 4.

J Virol Methods 2019 Jun 11;268:17-23. Epub 2019 Mar 11.

Department of Clinical Virology, University College London Hospitals NHS Foundation Trust, London, United Kingdom; National Transfusion Microbiology Laboratories, NHS Blood and Transplant, London, United Kingdom.

Dengue is a vector-transmitted viral infection that is a significant cause of morbidity and mortality in humans worldwide, with over 50 million apparent cases and a fatality rate of 2.5 % of 0.5 million severe cases per annum in recent years. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.006DOI Listing
June 2019
2 Reads

Identifying integration sites of the HIV-1 genome with intact and aberrant ends through deep sequencing.

J Virol Methods 2019 May 8;267:59-65. Epub 2019 Mar 8.

Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Aichi, 460-0001, Japan; Program in Integrated Molecular Medicine, Nagoya University Graduate School of Medicine, Nagoya, Aichi, 466-8550, Japan.

Paired-end deep sequencing is a powerful tool to investigate integration sites of the HIV-1 genome in infected cells. Integration sites of HIV-1 proviral DNA carrying intact LTR ends have been well documented. In contrast, integration sites of proviral DNA with aberrant ends, which emerge infrequently but can also induce replication-competent viruses, have not been extensively examined, in part, because of the lack of a suitable bioinformatics method for deep sequencing. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.004DOI Listing
May 2019
1 Read

High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy.

J Virol Methods 2019 May 6;267:66-70. Epub 2019 Mar 6.

Crop Research Institute, Drnovska 507, Praha 6, Ruzyne, 161 06, Czech Republic.

This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.003DOI Listing

Simultaneous detection of five pig viruses associated with enteric disease in pigs using EvaGreen real-time PCR combined with melting curve analysis.

J Virol Methods 2019 Jun 4;268:1-8. Epub 2019 Mar 4.

College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China. Electronic address:

In recent years, a series of porcine diarrhea viruses such as porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), rotaviruses of group A (RVA), rotaviruses of group C (RVC), and porcine circovirus 2 (PCV2) caused enormous economic losses all over the world. While any of these viruses is capable to cause disease alone, there is often concurrent infection with more than one virus on pig farms. In this study, a multiplex real-time PCR method based on EvaGreen fluorescent dye and melting curve analysis was established to simultaneously detect these five viruses in a single closed tube. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.001DOI Listing

Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assay with quenching primer for influenza virus and respiratory syncytial virus.

J Virol Methods 2019 May 1;267:53-58. Epub 2019 Mar 1.

Influenza Virus Research Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan. Electronic address:

Influenza virus and respiratory syncytial virus cause acute upper and lower respiratory tract infections, especially in children and the elderly. Early treatment for these infections is thought to be important, so simple and sensitive detection methods are needed for use at clinical sites. Therefore, in this study, real-time reverse transcription loop-mediated isothermal amplification assays with quenching primer for influenza virus and respiratory syncytial virus were developed. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.010DOI Listing
May 2019
4 Reads

Differential detection of porcine reproductive and respiratory syndrome virus genotypes by a fluorescence melting curve analysis using peptide nucleic acid probe-mediated one-step real-time RT-PCR.

J Virol Methods 2019 May 25;267:29-34. Epub 2019 Feb 25.

College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea. Electronic address:

Peptide nucleic acids (PNAs), artificially synthesized DNA analogues, hybridize strongly with DNA and are useful for fluorescence melting curve analyses (FMCA) based on the thermal denaturation of the probe-target duplex. In this study, we developed a PNA-based one-step real-time RT-PCR assay for the differential and qualitative detection of the porcine reproductive and respiratory syndrome virus genotypes PRRSV1 and PRRSV2. The specificity of the assay was analyzed in silico using previously reported primers and probes and was subsequently verified using Korean PRRSV panels and clinical samples. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.008DOI Listing

Determination of neutralization activities by a new versatile assay using an HIV-1 genome carrying the Gaussia luciferase gene.

J Virol Methods 2019 May 25;267:22-28. Epub 2019 Feb 25.

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, China; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA. Electronic address:

Characterization of neutralizing activities are critical to evaluation of the neutralization potency and breadth of monoclonal antibodies or anti-HIV-1 sera elicited during natural HIV-1 infection or by vaccines. We have developed a new neutralization method using the SG3Δenv genome carrying the Gaussia luciferase gene between the env and nef genes. Pseudotype viruses generated using this new SG3Δenv-GLuc backbone together with HIV-1 env genes were infectious to TZM-bl cells, T cell lines and primary T cells. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.009DOI Listing

Treatment of first-void urine with Aptima Transfer Solution increases detection of high-risk HPV E6/E7 mRNA.

J Virol Methods 2019 May 21;267:48-52. Epub 2019 Feb 21.

McMaster University, Hamilton, ON, Canada. Electronic address:

Because of its non-invasive nature urine testing may enable increased screening for HPV in women who avoid cervical sampling. Comparisons have shown fewer HPV positives in urine. The objectives were to compare first-void urine (FVU) treated with proteinase K (PK) to untreated FVU and cervical samples collected from women attending a colposcopy clinic using an Aptima HPV mRNA assay, and comparing the HPV rates to cytology and pathology results. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183047
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http://dx.doi.org/10.1016/j.jviromet.2019.02.007DOI Listing
May 2019
5 Reads

Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potato.

J Virol Methods 2019 May 20;267:16-21. Epub 2019 Feb 20.

Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, 53706, USA. Electronic address:

Potato virus Y (PVY) is a global challenge for potato production and the leading cause of seed crop downgrading and rejection for certification. Accurate and timely diagnosis is key to effective control of PVY. Here we optimized the isothermal recombinase polymerase amplification (RPA) for accurate detection of different PVY O and N types that were tested, present in different tissues of potato plants including tubers with a primer set that specifically targets the highly conserved pipo region within the viral genome. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.006DOI Listing

Added value of IgA antibodies against Zika virus non-structural protein 1 in the diagnosis of acute Zika virus infections.

J Virol Methods 2019 May 16;267:8-15. Epub 2019 Feb 16.

Institute for Experimental Immunology, Euroimmun AG, Seekamp 31, D-23560, Lübeck, Germany.

Zika virus (ZIKV) is a mosquito-borne flavivirus posing a public health threat due to its association with neurological complications in newborns and adults. In flavivirus-endemic areas, coming mosquito seasons will require the differentiation of primary versus secondary and acute versus past ZIKV/flavivirus infections. This is complicated by two major difficulties: [i] secondary infections often present with low or undetectable titres of specific IgM and with early-positive IgG, [ii] previous flavivirus infection(s) or vaccinations cause elevated cross-reactivities. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183043
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http://dx.doi.org/10.1016/j.jviromet.2019.02.005DOI Listing
May 2019
8 Reads
1.883 Impact Factor

In situ hybridization for the localization of two pepino mosaic virus isolates in mixed infections.

J Virol Methods 2019 May 13;267:42-47. Epub 2019 Feb 13.

Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Departamento de Biología del Estrés y Patología Vegetal, Murcia, Spain. Electronic address:

In situ hybridization (ISH) is an informative and relatively accessible technique for the localization of viral genomes in plant tissue and cells. However, simultaneous visualization of related plant viruses in mixed infections may be limited by the nucleotide similarity in the genomes and the single chromogenic detection over the same sample preparation. To address this issue, we used two Pepino mosaic virus isolates and performed ISH over consecutive serial cross-sections of paraffin-embedded leaf samples of single and mixed infected Nicotiana benthamiana plants. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.003DOI Listing
May 2019
1 Read

Localized surface plasmon resonance biosensing of tomato yellow leaf curl virus.

J Virol Methods 2019 May 13;267:1-7. Epub 2019 Feb 13.

Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.

Current techniques for plant virus detection, such as RT- PCR and ELISA, require multistep procedures and rely on sophisticated equipment. Due to the global spread of plant viruses, the development of simpler, faster and cheaper assay methods is inevitable. Gold nanoparticles (AuNPs) had raised much interest during recent years due to their novel optical properties or diagnostic purposes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183028
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http://dx.doi.org/10.1016/j.jviromet.2019.02.004DOI Listing
May 2019
11 Reads

Evaluation of a BioRad Avidity assay for identification of recent HIV-1 infections using dried serum or plasma spots.

J Virol Methods 2019 Apr 7;266:114-120. Epub 2019 Feb 7.

Division of HIV and Other Retroviruses, Robert Koch Institute, Berlin, Germany; Charité, Universitätsmedizin, Berlin, Berlin, Germany. Electronic address:

Serological methods to differentiate between recently acquired and established HIV-1 infections are a useful tool in the HIV-surveillance to characterize the epidemic, identify groups at risk and assess HIV-preventive interventions. Therefore, an avidity-based, modified BioRad Genscreen™ HIV-1/2 assay (BRA) was evaluated according to the avidity-based, modified BioRad HIV-1/2 Plus O protocol (BRA). Overall, 692 well defined samples (82. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.002DOI Listing
April 2019
1 Read

Impact of long-term storage of clinical samples collected from 1996 to 2017 on RT-PCR detection of norovirus.

J Virol Methods 2019 May 6;267:35-41. Epub 2019 Feb 6.

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 30333, USA. Electronic address:

Noroviruses are recognized as the leading cause of acute gastroenteritis globally. With improved molecular diagnostics developed over the last two decades, archived clinical specimens are increasingly used to investigate the historic prevalence and molecular epidemiology of human norovirus. Yet the impact of long-term storage on viral integrity in clinical specimens has not been evaluated. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.001DOI Listing
May 2019
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Multiplex real-time RT-PCR for detection and distinction of Spondweni and Zika virus.

J Virol Methods 2019 Apr 4;266:72-76. Epub 2019 Feb 4.

Centre of Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa; Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa. Electronic address:

Zika (ZIKV) and Spondweni viruses (SPOV) are closely related mosquito borne flaviviruses in the Spondweni serogroup. The co-circulation and similar disease presentation following ZIKV and SPOV infection necessitates the development of a diagnostic tool for their simultaneous detection and distinction. We developed a one-step multiplex real-time RT-PCR (ZIKSPOV) to detect and distinguish between SPOV and ZIKV by utilizing a single primer set combined with virus specific hydrolysis probes. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.011DOI Listing
April 2019
2 Reads

Using a new serotype-specific Polymerase Chain Reaction (PCR) and sequencing to differentiate between field and vaccine-derived African Horse Sickness viruses submitted in 2016/2017.

J Virol Methods 2019 Apr 2;266:89-94. Epub 2019 Feb 2.

Agricultural Research Council - Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa. Electronic address:

The outer capsid viral protein 2 (VP2) of African horse sickness virus, encoded by the most variable genome segment 2 (Seg-2), is the primary target for AHSV-specific neutralising antibodies and thus determines the virus serotype. Full length segment 2 sequences from more than 100 AHSVs isolated over the last 80 years were compared and single nucleotide polymorphisms (SNPs) identified between the reference strains and recent field viruses. Regions unique to each individual serotype were identified and primers designed to differentially amplify each of the nine serotypes. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.016DOI Listing
April 2019
5 Reads

The review of differential equation models of HBV infection dynamics.

Authors:
Miaolei Li Jian Zu

J Virol Methods 2019 Apr 1;266:103-113. Epub 2019 Feb 1.

School of Mathematics and Statistics, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China. Electronic address:

Understanding the infection and pathogenesis mechanism of hepatitis B virus (HBV) is very important for the prevention and treatment of hepatitis B. Mathematical models contribute to illuminate the dynamic process of HBV replication in vivo. Therefore, in this paper we review the viral dynamics in HBV infection, which may help us further understand the dynamic mechanism of HBV infection and efficacy of antiviral treatment. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.014DOI Listing
April 2019
2 Reads

Evaluation of different genomic regions of Rotavirus A for development of real time PCR.

J Virol Methods 2019 Apr 30;266:65-71. Epub 2019 Jan 30.

Enteric Viruses Group, ICMR-National Institute of Virology, Pune, India. Electronic address:

The nucleotide alignment of all 11 genes of human Rotavirus A (RVA) strains revealed suitability of NSP2, NSP3 and VP6 genes for the development of real time PCR (qRT-PCR). Evaluation of qRT-PCR assays using known rotavirus ELISA positive and negative fecal specimens showed non-overlapping ranges of Mean ±3SD cycle threshold (Ct) values for NSP3 and VP6 based assays. Using serial dilutions of purified RVA, high sensitivity of VP6 qRT-PCR assay (1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183058
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http://dx.doi.org/10.1016/j.jviromet.2019.01.017DOI Listing
April 2019
12 Reads

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.

J Virol Methods 2019 Apr 28;266:58-64. Epub 2019 Jan 28.

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Mixed infections with different pathogens are common in sheep and goats under intensive production conditions. Quick and accurate detection and differentiation of different pathogens is necessary for epidemiological surveillance, disease management and import and export controls. Multiplex TaqMan qPCR protocols were developed and subsequently evaluated as effective tools in simultaneously detecting single and mixed infections in sheep and goats. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.015DOI Listing
April 2019
2 Reads
1.883 Impact Factor

Application of deep sequencing methods for inferring viral population diversity.

J Virol Methods 2019 Apr 25;266:95-102. Epub 2019 Jan 25.

Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan; Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan. Electronic address:

The first deep sequencing method was announced in 2005. Due to an increasing number of sequencing data and a reduction in the costs of each sequencing dataset, this innovative technique was soon applied to genetic investigations of viral genome diversity in various viruses, particularly RNA viruses. These deep sequencing findings documented viral epidemiology and evolution and provided high-resolution data on the genetic changes in viral populations. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.013DOI Listing
April 2019
1 Read

Validation of an immunoblot assay employing an objective reading system and used as a confirmatory test in equine infectious anaemia surveillance programs.

J Virol Methods 2019 Apr 23;266:77-88. Epub 2019 Jan 23.

Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Via Appia Nuova 1411, 00178, Rome, Italy. Electronic address:

Equine infectious anaemia (EIA) is a blood borne disease that is listed among the notifiable diseases of the World Organisation for Animal Health (OIE). EIA is also regulated by the OIE for the international trading provisions and is generally subject to control programmes. Since 2011, Italy has been conducting a surveillance plan based on a three-tier diagnostic system, using a serological ELISA as screening test, an agar gel immunodiffusion test (AGIDT) as a confirmatory method, and an immunoblot (IB) as an alternative confirmatory assay for discordant results between the first two tests. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.012DOI Listing
April 2019
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Evaluation of two workflows for whole genome sequencing-based typing of influenza A viruses.

J Virol Methods 2019 Apr 21;266:30-33. Epub 2019 Jan 21.

Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address:

We compared two sample preparation protocols for whole genome sequencing of influenza A viruses. Each protocol was assessed using cDNA quantity and quality and the resulting mean genome coverage after sequencing. Both protocols produced acceptable result for samples with high viral load, whereas one protocol performed slightly better with limited virus count. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.009DOI Listing

Rapid and sensitive detection of goose parvovirus and duck-origin novel goose parvovirus by recombinase polymerase amplification combined with a vertical flow visualization strip.

J Virol Methods 2019 Apr 22;266:34-40. Epub 2019 Jan 22.

Zhongkai University of Agriculture and Engineering, Guangdong, Guangzhou, 510225, China; Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangdong, Guangzhou, 510225, China. Electronic address:

Goose parvovirus (GPV) is one of the most serious viral pathogens in goslings. Recently, a new pathogen to the Chinese mainland-duck-origin novel goose parvovirus (N-GPV)-was found to be 90.8-94. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183058
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http://dx.doi.org/10.1016/j.jviromet.2019.01.010DOI Listing
April 2019
8 Reads