8,084 results match your criteria Journal of Neuroscience Methods [Journal]


Unsupervised quantification of tissue immunofluorescence in animal models of multiple sclerosis - instructions for use.

J Neurosci Methods 2019 Mar 12. Epub 2019 Mar 12.

Department of Neurology, Goethe University Hospital, Frankfurt am Main, Germany.

Background: In the analysis of animal models of CNS diseases such as experimental autoimmune encephalomyelitis (EAE), immunostaining and histopathology are important readouts. However, the complex morphological features of a tissue staining are often reduced to a single measure which relies on tedious manual planimetry. Furthermore, the measure itself and co-variables such as the region being analysed are chosen in a human decision-making process, which introduces bias. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.003DOI Listing

A simple method to study motor and non-motor behaviors in adult zebrafish.

J Neurosci Methods 2019 Mar 11. Epub 2019 Mar 11.

Zebrafish Genetics Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, 603 203, India. Electronic address:

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.008DOI Listing

Long term reliability of nociceptive withdrawal reflex thresholds.

J Neurosci Methods 2019 Mar 11. Epub 2019 Mar 11.

Center for Interdisciplinary Pain Medicine, Department of Anesthesiology, University Hospital of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany; University Hospital of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.

Background: The nociceptive withdrawal reflex (NWR) is a polysynaptic spinal reflex protecting the body from harmful stimuli. Two different methods to assess its' threshold (NWR-T) have been part of clinical trials concerning the evaluation of the nociceptive system in the human body. NWR-T's are gathered by stimulation at the sole of the foot and over the sural pathway. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.009DOI Listing

Methods and Models in Alzheimer's Disease.

Authors:
Bettina Platt

J Neurosci Methods 2019 Mar 11. Epub 2019 Mar 11.

Translational Neuroscience, University of Aberdeen, United Kingdom. Electronic address:

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.007DOI Listing

Neurofilament-histomorphometry comparison in the evaluation of unmyelinated axon regeneration following peripheral nerve injury: an alternative to electron microscopy.

J Neurosci Methods 2019 Mar 6. Epub 2019 Mar 6.

Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada; Department of Surgery, University of Toronto, 149 College Street, 5th floor, Toronto, ON, M5T 1P5, Canada; Institute of Medical Science, University of Toronto, 1 King's College Circle, Room 2374, Toronto, ON, M5S 1A8, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON, M5S 3G9, Canada. Electronic address:

Background: Currently, assessment of unmyelinated axon regeneration is limited to electron microscopy (EM), which is expensive, time consuming and not universally available. This study presents a protocol to estimate the number of unmyelinated axons in a regenerating peripheral nerve without the need for electron microscopy.

New Method: The common peroneal nerve of Sprague-Dawley rats was transected, repaired and regenerated for 4 weeks. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.006DOI Listing
March 2019
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Synchrotron XRF imaging of Alzheimer's disease basal ganglia reveals linear dependence of high-field magnetic resonance microscopy on tissue iron concentration.

J Neurosci Methods 2019 Mar 6. Epub 2019 Mar 6.

School of Engineering, University of Warwick, Coventry, CV4 7AL, UK; Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA. Electronic address:

Background: Chemical imaging of the human brain has great potential for diagnostic and monitoring purposes. The heterogeneity of human brain iron distribution, and alterations to this distribution in Alzheimer's disease, indicate iron as a potential endogenous marker. The influence of iron on certain magnetic resonance imaging (MRI) parameters increases with magnetic field, but is under-explored in human brain tissues above 7 T. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.002DOI Listing
March 2019
1 Read

ReStore: a wireless peripheral nerve stimulation system.

J Neurosci Methods 2019 Mar 5. Epub 2019 Mar 5.

The University of Texas at Dallas, Erik Jonsson School of Engineering and Computer Science, Department of Bioengineering, 800 West Campbell Road, Richardson, TX 75080-3021, United States; The University of Texas at Dallas, Texas Biomedical Device Center, 800 West Campbell Road, Richardson, TX 75080-3021, United States; The University of Texas at Dallas, School of Behavioral Brain Sciences, 800 West Campbell Road, GR41, Richardson, TX 75080-3021, United States. Electronic address:

Background: The growing use of neuromodulation techniques to treat neurological disorders has motivated efforts to improve on the safety and reliability of implantable nerve stimulators.

New Method: The present study describes the ReStore system, a miniature, implantable wireless nerve stimulator system that has no battery or leads and is constructed using commercial components and processes. The implant can be programmed wirelessly to deliver charge-balanced, biphasic current pulses of varying amplitudes, pulse widths, frequencies, and train durations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270193005
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http://dx.doi.org/10.1016/j.jneumeth.2019.02.010DOI Listing
March 2019
1 Read

Comparative study of extracellular recording methods for analysis of afferent sensory information: Empirical modeling, data analysis and interpretation.

J Neurosci Methods 2019 Mar 5. Epub 2019 Mar 5.

Bioengineering Institute, Miguel Hernández University (UMH), Alicante, Spain; Biomedical Research Networking center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Zaragoza, Spain. Electronic address:

Background: Physiological studies of sensorial systems often require the acquisition and processing of data extracted from their multiple components to evaluate how the neural information changes in relation to the environment changes. In this work, a comparative study about methodological aspects of two electrophysiological approaches is described.

New Method: Extracellular recordings from deep vibrissal nerves were obtained by using a customized microelectrode Utah array during passive mechanical stimulation of rat´s whiskers. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270193007
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http://dx.doi.org/10.1016/j.jneumeth.2019.03.004DOI Listing
March 2019
2 Reads

Evaluating the applicability of mouse SINEs as an alternative normalization approach for RT-qPCR in brain tissue of the APP23 model for Alzheimer's disease.

J Neurosci Methods 2019 Mar 5. Epub 2019 Mar 5.

Laboratory of Neurochemistry and Behavior, Institute Born-Bunge, University of Antwerp, Universiteitsplein 1, BE-2610 Wilrijk, Belgium; Department of Neurology and Alzheimer Research Center, University Medical Center Groningen (UMCG), Hanzeplein 1, 9713 GZ Groningen, The Netherlands; Department of Neurology and Memory Clinic, Hospital Network Antwerp (ZNA) Middelheim and Hoge Beuken, Lindendreef 1, BE-2020 Antwerp, Belgium; Biobank, Institute Born-Bunge, University of Antwerp, Universiteitsplein 1, BE-2610 Antwerp, Belgium.

Background: The choice of appropriate reference genes (RGs) for use in reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been thoroughly investigated, since the inclusion of unstable RGs might cause inaccurate gene expression results.

New Method: Short interspersed nuclear elements (SINEs) such as B elements, might represent an alternative solution given the high occurrence of these repetitive elements in the rodent genome and transcriptome. We performed RT-qPCR to investigate the stability of nine commonly used RGs and two B elements, B1 and B2, across different age- and genotype-related experimental conditions in the hippocampus and cortex of the APP23 amyloidosis mouse model for Alzheimer's disease. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.005DOI Listing
March 2019
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SEXRAT MALE: A smartphone and tablet application to annotate and process live sexual behavior in male rodents.

J Neurosci Methods 2019 Mar 2;320:9-15. Epub 2019 Mar 2.

Neuroscience Laboratory, Department of Psychology, Universidad Iberoamericana, Mexico City, Mexico.

Background: In behavioral neuroscience studies, it is essential to accurately and easily record rodent sexual behavior. However, current systems are complex, expensive and often require a computer that hinders their use in situ.

New Method: SEXRAT MALE is new software for the real-time annotation and process of live sexual behavior in male rodents. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.03.001DOI Listing

Overlapping brain Community detection using Bayesian tensor decomposition.

J Neurosci Methods 2019 Mar 1;318:47-55. Epub 2019 Mar 1.

CIPCE, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran, Iran; Departments of Radiology and Research Administration, Henry Ford Health System, Detroit, MI, USA. Electronic address:

It has been found that specific regions in the brain are dedicated to specific functions. Detection and analysis of the constituent functional networks of the brain is of great importance for understanding the brain functionality and diagnosing some neuropsychiatric illnesses. In this paper, we introduce Non-negative Tensor Factorization (NTF) methods to identify the overlapping communities in brain networks using resting-state functional Magnetic Resonance Imaging (rs-fMRI) data. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.014DOI Listing

Comparison of the efficiency of chopped and non-rectangular electrical stimulus waveforms in activating small vagus nerve fibers.

J Neurosci Methods 2019 Mar 1;320:1-8. Epub 2019 Mar 1.

INRIA, University of Montpellier, Montpellier, France.

Background: In the context of morbid obesity, vagus nerve stimulation could be used to control gastric function targeting the small afferent B-fibers and C-fibers. Compared to large A-fibers, activation thresholds of these small efferent fibers are 10 to 100 times greater, inducing technical constraints and possible nerve damages. Although rectangular waveform is commonly used in nerve stimulation, recent modeling and experimental studies suggest that non-rectangular waveforms could reduced the charge injected by the stimulator. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.017DOI Listing

Exploring directed functional connectivity based on electroencephalography source signals using a global cortex factor-based multivariate autoregressive model.

J Neurosci Methods 2019 Feb 25;318:6-16. Epub 2019 Feb 25.

College of Information Science and Technology, Beijing Normal University, Beijing, 100875, China.

Background: Partial directed coherence (PDC) computed from multivariate autoregressive (MVAR) model coefficient is increasingly being used to study directed functional connectivity between brain regions in the frequency domain based on electroencephalography (EEG) source signals. However, directly fitting MVAR model to the high-dimensional source signals is difficult. Besides, although PDC measurement often shows good results for simulated data, it is not clear to what extent the results for real data are physiologically plausible. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.016DOI Listing
February 2019

Unsupervised identification of states from voltage recordings of neural networks.

J Neurosci Methods 2019 Feb 23. Epub 2019 Feb 23.

Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. Electronic address:

Background: Modern techniques for multi-neuronal recording produce large amounts of data. There is no automatic procedure for the identification of states in recurrent voltage patterns.

New Method: We propose NetSAP (Network States And Pathways), a data-driven analysis method that is able to recognize multi-neuron voltage patterns (states). Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.019DOI Listing
February 2019

A novel control condition for spatial learning in the Morris water maze.

J Neurosci Methods 2019 Feb 23;318:1-5. Epub 2019 Feb 23.

Department of Psychology, Maynooth University, Co. Kildare, Ireland.

Background: Assessing markers of neural activity in rodent behavioural tasks benefits from meaningful comparison with matched control conditions. The Morris water maze is a widely used learning and memory task, but currently implemented control conditions are not optimal.

New Method: We created a novel control condition involving random escape platform placement for each trial, while increasing the frequency of platforms throughout the training period. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.015DOI Listing
February 2019

EEG spatiospectral patterns and their link to fMRI BOLD signal via variable hemodynamic response functions.

J Neurosci Methods 2019 Feb 22;318:34-46. Epub 2019 Feb 22.

Department of Biomedical Engineering, Brno University of Technology, Technická 12, Brno, 61600, Czech Republic.

Background: Spatial and temporal resolution of brain network activity can be improved by combining different modalities. Functional Magnetic Resonance Imaging (fMRI) provides full brain coverage with limited temporal resolution, while electroencephalography (EEG), estimates cortical activity with high temporal resolution. Combining them may provide improved network characterization. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.012DOI Listing
February 2019

Straightforward method for singularized and region-specific CNS microvessels isolation.

J Neurosci Methods 2019 Feb 21;318:17-33. Epub 2019 Feb 21.

University of California, Davis. Anatomy, Physiology & Cell Biology, 1089 Veterinary Medicine Drive, Davis, CA, 95616, United States. Electronic address:

Background: Current methods for murine brain microvasculature isolation requires the pooling of brain cortices while disregarding the rest of the CNS, making the analysis of single individuals non feasible.

New Method: Efficient isolation of brain microvessels requires the elimination of meninges, vessels of high caliber vessels and choroid plexus, commonly done by rolling the over filter paper, but can't be done on other CNS regions. We overcome this hurdle by using a double-pronged pick, as well as elution and filtration through cell strainers after centrifugation. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.007DOI Listing
February 2019

Reversible external auditory canal ligation (REACL): A novel surgical technique to induce transient and reversible hearing loss in developing rats.

J Neurosci Methods 2019 Apr 18;317:108-112. Epub 2019 Feb 18.

Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC, Canada. Electronic address:

Background: Cases of sensory loss provide an excellent model to study the plastic nature of cortical sensory systems. Models of reversible sensory loss are particularly useful for establishing the timeline of various critical periods for cortical plasticity. However, there currently is an absence of adequate methods to produce reversible hearing loss in neonatal and developing rodents. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.011DOI Listing

Reconstruction of effective connectivity in the case of asymmetric phase distributions.

J Neurosci Methods 2019 Apr 17;317:94-107. Epub 2019 Feb 17.

University of Cologne, Institute of Zoology, Heisenberg Research Group of Computational Neuroscience - Modeling Neural Network Function, Zülpicher Str. 47b, 50674 Cologne, Germany; Research Centre Jülich, Institute of Neuroscience and Medicine (INM-3), Cognitive Neuroscience, 52425 Jülich, Germany. Electronic address:

Background: The interaction of different brain regions is supported by transient synchronization between neural oscillations at different frequencies. Different measures based on synchronization theory are used to assess the strength of the interactions from experimental data. One method of estimating the effective connectivity between brain regions, within the framework of the theory of weakly coupled phase oscillators, was implemented in Dynamic Causal Modelling (DCM) for phase coupling (Penny et al. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.009DOI Listing
April 2019
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A multidimensional artefact-reduction approach to increase robustness of first-level fMRI analyses: Censoring vs. interpolating.

J Neurosci Methods 2019 Feb 16;318:56-68. Epub 2019 Feb 16.

Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child Health, United Kingdom; Great Ormond Street Hospital NHS Trust, London, United Kingdom.

Background: This manuscript describes a new, multidimensional and data-driven approach to identify outlying datapoints from a first-level fMRI dataset.

New Method: Using three different indicators of data corruption (the fast variance component of DVARS [Δ%D-var], scan-to-scan total displacement [STS], and each scan's overall explained variance [R]), it identifies outlying datapoints while being balanced using Akaike'c corrected criterion (AIC ) to avoid overcorrection. We then explore the impact of censoring, interpolating, or both, to remove a bad scan's contribution to the final timeseries. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.008DOI Listing
February 2019
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A palm-worn device to quantify rigidity in Parkinson's disease.

J Neurosci Methods 2019 Apr 15;317:113-120. Epub 2019 Feb 15.

Bionics Institute, East Melbourne, Victoria, Australia; Department of Medical Bionics, University of Melbourne, East Melbourne, Victoria, Australia.

Background: Parkinsonian rigidity is identified on clinical examination as resistance to passive movement. Measurement of rigidity commonly relies on ordinal rating scales (MDS-UPDRS), however instrumented objective measures may provide greater mechanistic insight.

New Method: We present a palm-worn instrument to objectively quantify rigidity on a continuous scale. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.006DOI Listing
April 2019
4 Reads

Development of a non-human primate model to support CNS translational research: Demonstration with D-amphetamine exposure and dopamine response.

J Neurosci Methods 2019 Apr 12;317:71-81. Epub 2019 Feb 12.

School of Pharmacy and Graduate School of Pharmaceutical Sciences, Division of Pharmaceutical, Administrative and Social Sciences, Duquesne University, Pittsburgh, PA, USA. Electronic address:

Background: Challenges specific to the discovery and development of candidate CNS drugs have led to implementation of various in silico, in vitro and in vivo approaches to improve the odds for commercialization of novel treatments.

New Method: Advances in analytical methodology and microdialysis probe design have enabled development of a non-human primate model capable of measuring concentrations of drugs or endogenous chemicals in brain extracellular fluid (ECF) and cerebrospinal fluid (CSF). Linking these to population modeling reduces animal numbers to support predictive translational sciences in primates. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.005DOI Listing
April 2019
1 Read

An arterially perfused brainstem preparation of guinea pig to study central mechanisms of airway defense.

J Neurosci Methods 2019 Apr 8;317:49-60. Epub 2019 Feb 8.

Florey Institute of Neuroscience and Mental Health, Gate 11 Royal Parade, 3052 Parkville, University of Melbourne Victoria, Australia.

The perfused working heart brainstem preparation of rodents has become a widely used tool to study brainstem function. Here, we adapt this experimental technique for newborn guinea pigs (postnatal day 7-14) to develop a tool that enables investigation of airway defense mechanisms not observed in other rodents. The perfused guinea pig brainstem preparation generates a stable eupnea-like motor pattern recorded from the phrenic, recurrent laryngeal and intercostal nerves and basic cardio-respiratory reflexes, including the arterial chemoreceptor, the baroreceptor reflex. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.004DOI Listing

Visualization of intrathecal delivery by PET-imaging.

J Neurosci Methods 2019 Apr 7;317:45-48. Epub 2019 Feb 7.

CENSE, Department of Neurosurgery, Aarhus University Hospital, Denmark.

Background: Intrathecal (IT) delivery is useful in both basic research and clinical treatments. Here we aim to test a new minimally invasive distribution route to the subarachnoid space (SAS) and the flow of IT administrations. We placed a radioligand into SAS during positron emission tomography (PET) scanning as a proof of concept. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.003DOI Listing

Iterative expert-in-the-loop classification of sleep PSG recordings using a hierarchical clustering.

J Neurosci Methods 2019 Apr 7;317:61-70. Epub 2019 Feb 7.

Czech Institute of Informatics, Robotics, and Cybernetics, Czech Technical University in Prague, Czech Republic; Faculty of Biomedical Engineering, Czech Technical University in Prague, Czech Republic.

Background: The classification of sleep signals is a subjective and time consuming task. A large number of automatic classifiers have been published in the past decade but a sleep community has no strong confidence to use them in clinical practice and still remains using a standard manual scoring according standardized rules.

New Method: We developed a semi-supervised data-driven approach for objective and efficient evaluation of polysomnographic (PSG) data. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.013DOI Listing
April 2019
2 Reads

Effective chair training methods for neuroscience research involving rhesus macaques (Macaca mulatta).

J Neurosci Methods 2019 Apr 6;317:82-93. Epub 2019 Feb 6.

Department of Experimental Psychology, University of Oxford, Oxford, OX1 3SR, United Kingdom. Electronic address:

Background: Neuroscience research using macaques remains fundamental in our endeavours to understand how the human brain functions. Applying the refinement principle of the 3Rs is essential to optimise the monkeys' welfare and still produces high quality science.

New Method: Here we document refinements in our training methods for acclimation to transport devices (i. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.02.001DOI Listing
April 2019
1 Read

Vibrotactile piezoelectric stimulation system with precise and versatile timing control for somatosensory research.

J Neurosci Methods 2019 Apr 7;317:29-36. Epub 2019 Feb 7.

Division of Newborn Medicine, Department of Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

Background: Tactile stimulations systems are useful for studying the somatosensory system in children because they are innocuous and safe. Stimulators based on piezoelectric actuator are useful, but there is still a need for such systems capable of providing accurate and versatile control of timing and pattern of activation.

New Method: We have implemented a vibrotactile stimulating system useful for behavioral and electroencephalography (EEG) and magnetoencephalography (MEG) research. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270193004
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http://dx.doi.org/10.1016/j.jneumeth.2019.02.002DOI Listing
April 2019
9 Reads

A Novel Texture Extraction Technique with T1 Weighted MRI for the Classification of Alzheimer's Disease.

J Neurosci Methods 2019 Feb 5. Epub 2019 Feb 5.

Department of Computer Science and Engineering, Community College, Pondicherry University, Puducherry, India.

Background: As the medical images contain both superficial and imperceptible patterns, textures are successfully used as discriminant features for the detection of cancers, tumors, etc. NEW METHOD: Our algorithm selects the specific image blocks and computes the textures using the following steps: At first, the center image slice of the axes (sagittal, coronal and axial) is divided into small blocks and those which approximately resembles the regions of interest are marked. Then, all the marked blocks which are in the same location as in the center slice are collected from all the other slices, and the textures are computed per block on all the individual slices. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.011DOI Listing
February 2019
1 Read

Monitoring deep brain stimulation by measuring regional brain oxygen responses in freely moving mice.

J Neurosci Methods 2019 Apr 1;317:20-28. Epub 2019 Feb 1.

Netherlands Institute for Neuroscience, an institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, the Netherlands; Department of Psychiatry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands.

Background: Translational studies investigating the effects of deep brain stimulation (DBS) on brain function up to now mainly relied on BOLD responses measured with fMRI. However, fMRI studies in rodents face technical and practical limitations (e.g. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.012DOI Listing
April 2019
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Event-related potential arithmetic to analyze offset potentials from conscious mice.

Authors:
Jamie A O'Reilly

J Neurosci Methods 2019 Jan 31. Epub 2019 Jan 31.

College of Biomedical Engineering, Rangsit Univerity, Pathumthani, Thailand; Department of Biomedical Engineering, University of Strathclyde, Glasgow, UK; Strathclyde Institute of Pharmacy and Biomedical Science, University of Strathclyde, Glasgow, UK. Electronic address:

Background: This paper presents a method for isolating time-dependent event-related potential (ERP) components which are superimposed on the gross ERP waveform. The experimental data that inspired this approach was recorded from the auditory cortex of conscious laboratory mice in response to presentation of ten different duration pure-tone auditory stimuli.

New Method: The grand-average ERP for each individual stimulus displayed a relatively low amplitude deflection following stimulus offset. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.018DOI Listing
January 2019

Behavioral neuroimaging in birds using PET.

J Neurosci Methods 2019 Apr 30;317:157-164. Epub 2019 Jan 30.

Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794-5230, USA; Department of Radiology, Renaissance School of Medicine at Stony Brook University, Stony Brook, NY, USA. Electronic address:

Background: Birds comprise the most diverse group of terrestrial vertebrates. This success likely is related to the evolution of powered flight over 75 mya. Modern approaches for studying brain function, however, have yet to be fully adapted and applied to birds, especially as they relate to specific behaviors including flight. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.017DOI Listing
April 2019
1 Read

Delay differential analysis for dynamical sleep spindle detection.

J Neurosci Methods 2019 Mar 30;316:12-21. Epub 2019 Jan 30.

Computational Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Institute for Neural Computation, University of California San Diego, La Jolla, CA 92093, USA; Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.

Background: Sleep spindles are involved in memory consolidation and other cognitive functions. Numerous automated methods for detection of spindles have been proposed; most of these rely on spectral analysis in some form. However, none of these approaches are ideal, and novel approaches to the problem could provide additional insights. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.009DOI Listing
March 2019
1 Read

A novel rat model of cerebral artery occlusion complicated with prior venous stagnation.

J Neurosci Methods 2019 Jan 28. Epub 2019 Jan 28.

Department of Neurology, General Hospital of Shenyang Military Region, Shenyang, 110840, PR China. Electronic address:

Background: To establish a novel rat model of middle cerebral artery occlusion (MCAO) complicated with prior venous stagnation, and to investigate the role of cerebral venous drainage in neural injury after acute cerebral infarction.

New Method: Eighteen SD rats were randomly divided into two groups: control group and jugular vein ligation group. The left jugular vein ligation was performed to produce the jugular venous stagnation. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.016DOI Listing
January 2019

Antibodies raised against aldehyde-fixed antigens improve sensitivity for postembedding electron microscopy.

J Neurosci Methods 2019 Apr 28;317:1-10. Epub 2019 Jan 28.

Division of Anatomy, Department of Molecular Medicine, Institute of Basic Medical Science, University of Oslo, Oslo, Norway.

Background: Antibodies are one of the most important tools in biological research. High specificity and sensitivity of antibodies are crucial to obtain reliable results. Tissue fixed with glutaraldehyde (GA) is commonly used in electron microscopical investigations. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.015DOI Listing

F-FDG positron emission tomography as a novel diagnostic tool for peripheral nerve injury.

J Neurosci Methods 2019 Apr 23;317:11-19. Epub 2019 Jan 23.

Department of Rehabilitation Medicine, Seoul National University Boramae Medical Center, 425 Sindaebang-dong, Dongjak-gu, Seoul 156-707, Republic of Korea. Electronic address:

Background: Glucose hypermetabolism in denervated skeletal muscle suggests the potential for developing a diagnostic tool for peripheral nerve injuries. Herein, we investigated the characteristics and molecular mechanism of this phenomenon.

New Method: Temporal course of glucose hypermetabolism and development of abnormal spontaneous activities (ASA) through electromyography (EMG) were investigated in rats with complete sciatic nerve injuries. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270193002
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http://dx.doi.org/10.1016/j.jneumeth.2019.01.014DOI Listing
April 2019
6 Reads

Rapid and high-efficient generation of mutant mice using freeze-thawed embryos of the C57BL/6J strain.

J Neurosci Methods 2019 Apr 23;317:149-156. Epub 2019 Jan 23.

Graduate School of Innovative Life Science, University of Toyama, Toyama, 930-0194, Japan; Life Science Research Center, University of Toyama, Toyama, 930-0194, Japan.

Background: The CRISPR/Cas9 technique has undergone many modifications to decrease the effort and shorten the time needed for efficient production of mutant mice. The use of fresh embryos consumes time and effort during oocytes preparation and fertilization before every experiment, and freeze-thawed embryos overcome this limitation. However, cryopreservation of 1-cell embryos is challenging. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.010DOI Listing
April 2019
2 Reads

Joint modeling of reaction times and choice improves parameter identifiability in reinforcement learning models.

J Neurosci Methods 2019 Apr 18;317:37-44. Epub 2019 Jan 18.

Department of Psychology, Arizona State University, Tempe, AZ 85287, USA.

Background: Reinforcement learning models provide excellent descriptions of learning in multiple species across a variety of tasks. Many researchers are interested in relating parameters of reinforcement learning models to neural measures, psychological variables or experimental manipulations. We demonstrate that parameter identification is difficult because a range of parameter values provide approximately equal quality fits to data. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.006DOI Listing
April 2019
1 Read

A graphical user interface to assess the neuroinflammatory response to intracortical microelectrodes.

J Neurosci Methods 2019 Apr 18;317:141-148. Epub 2019 Jan 18.

Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, United States; Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, United States. Electronic address:

Background: Brain-implanted devices, including intracortical microelectrodes, are used in neuroscience applications ranging from research to rehabilitation and beyond. Significant efforts are focused on developing new device designs and insertion strategies that mitigate initial trauma and subsequent neuroinflammation that occurs as a result of implantation. A frequently published metric is the neuroinflammatory response quantified as a function of distance from the interface edge, using fluorescent immunohistochemical markers. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.003DOI Listing
April 2019
2 Reads

An efficient EEG based deceit identification test using wavelet packet transform and linear discriminant analysis.

J Neurosci Methods 2019 Feb 17;314:31-40. Epub 2019 Jan 17.

Department of Computer Science and Engineering, National Institute of Technology, Goa, India. Electronic address:

Background: Brain-computer interface (BCI) is a combination of hardware and software that provides a non-muscular channel to send various messages and commands to the outside world and control external devices such as computers. BCI helps severely disabled patients having neuromuscular injuries, locked-in syndrome (LiS) to lead their life as a normal person to the best extent possible. There are various applications of BCI not only in the field of medicine but also in entertainment, lie detection, gaming, etc. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.007DOI Listing
February 2019
4 Reads

Accessory to dissipate heat from transcranial magnetic stimulation coils.

J Neurosci Methods 2019 Feb 16;314:28-30. Epub 2019 Jan 16.

Bloorview Research Institute, Holland Bloorview Kids Rehabilitation Hospital, Toronto, Ontario, Canada; Department of Speech-Language Pathology, Faculty of Medicine, University of Toronto, Ontario, Canada.

Background: Transcranial magnetic stimulation (TMS) produces magnetic pulses by passing a strong electrical current through coils of wire. Repeated stimulation accumulates heat, which places practical constraints on experimental design. New method: We designed a condensation-free pre-chilled heat sink to extend the operational duration of transcranial magnetic stimulation coils. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.008DOI Listing
February 2019
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Multiple-command single-frequency SSVEP-based BCI system using flickering action video.

J Neurosci Methods 2019 Feb 16;314:21-27. Epub 2019 Jan 16.

Department of Biomedical Engineering, College of Medicine, Keimyung University, South Korea. Electronic address:

Background: The number of commands in a brain-computer interface (BCI) system is important. This study proposes a new BCI technique to increase the number of commands in a single BCI system without loss of accuracy.

New Method: We expected that a flickering action video with left and right elbow movements could simultaneously activate the different pattern of event-related desynchronization (ERD) according to the video contents (e. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.005DOI Listing
February 2019
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Quantitatively detecting postictal hypoperfusion in patients with focal epilepsy using CT perfusion: Determining cross-modality comparisons and electrode artifacts.

J Neurosci Methods 2019 Feb 15;314:13-20. Epub 2019 Jan 15.

Hotchkiss Brain Institute, University of Calgary, Canada; Seaman Family MR Research Centre, University of Calgary, Canada; Department of Clinical Neurosciences, University of Calgary, Canada; Department of Radiology, University of Calgary, Canada. Electronic address:

Background: We previously showed that CT perfusion (CTP) and arterial spin labelled (ASL) MRI can localize the seizure onset zone in humans via postictal perfusion patterns. As a step towards improving the feasibility/ease of collecting postictal CBF data, we determined whether EEG electrodes need to be removed for CTP data collection and whether a cross-modality comparison between baseline ASL and postictal CTP data is possible.

New Method: Five patients with epilepsy underwent postictal CTP scanning. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.004DOI Listing
February 2019
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A novel low-noise movement tracking system with real-time analog output for closed-loop experiments.

J Neurosci Methods 2019 Jan 14. Epub 2019 Jan 14.

Sagol School of Neuroscience and Department of Physiology and Pharmacology, Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address:

Background: Modern electrophysiological experiments are moving towards closing the loop, where the extrinsic (behavioral) and intrinsic (neuronal) variables automatically affect stimulation parameters. Rodent experiments targeting spatial behavior require animal 2D kinematics to be continuously monitored in a reliable and accurate manner. Cameras provide a robust, flexible, and simple way to track kinematics on the fly. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2018.12.016DOI Listing
January 2019
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Measurement of block thresholds in kiloHertz frequency alternating current peripheral nerve block.

J Neurosci Methods 2019 Mar 11;315:48-54. Epub 2019 Jan 11.

Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA; MetroHealth Medical Center, 2500 MetroHealth Drive, Cleveland, OH, 44109, USA. Electronic address:

Background: Kilohertz frequency alternating currents (KHFAC) produce rapid nerve conduction block of mammalian peripheral nerve and have potential clinical applications in reducing peripheral nerve hyperactivity. The experimental investigation of KHFAC nerve block requires a robust output measure and this has proven to be the block threshold (BT), the lowest current or voltage at which the axons of interest are completely blocked. All significant literature in KHFAC nerve block, both simulations and experimental, were reviewed to determine the block threshold method that was used. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380354PMC
March 2019
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An evaluation of Drosophila as a model system for studying tauopathies such as Alzheimer's disease.

J Neurosci Methods 2019 Jan 8. Epub 2019 Jan 8.

School of Natural Sciences, Bangor University, Bangor, Gwynedd, UK.

Work spanning almost two decades using the fruit fly, Drosophila melanogaster, to study tau-mediated neurodegeneration has provided valuable and novel insights into the causes and mechanisms of tau-mediated toxicity and dysfunction in tauopathies such as Alzheimer's disease (AD). The fly has proven to be an excellent model for human diseases because of its cost efficiency, and the availability of powerful genetic tools for use in a comparatively less-complicated, but evolutionarily conserved, in vivo system. In this review, we provide a critical evaluation of the insights provided by fly models, highlighting both the advantages and limitations of the system. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2019.01.001DOI Listing
January 2019
3 Reads

Evaluation of select biocompatible markers for labelling peripheral nerves on 11.7 T MRI.

J Neurosci Methods 2019 Mar 7;315:6-13. Epub 2019 Jan 7.

Department of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Background: Peripheral nerve injury is often followed by a highly variable recovery process with respect to both rapidity and efficacy. Identifying post-nerve injury phenomena is key to assessing the merit and timing of surgery as well as to tracking nerve recovery postoperatively. Diffusion Tensor Imaging (DTI) has been investigated in the clinical and research settings as a noninvasive technique to both assess and monitor each patient's unique case of peripheral nerve damage. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270183041
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http://dx.doi.org/10.1016/j.jneumeth.2018.12.019DOI Listing
March 2019
8 Reads

Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage.

J Neurosci Methods 2019 Mar 6;315:1-5. Epub 2019 Jan 6.

Unit of Medical Pharmacy, Department of Pharmacy Practice, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.

Background: Microdialysis (MD) is conventionally used to measure the in vivo levels of various substances and metabolites in extracellular and cerebrospinal fluid of brain. However, insertion of the MD probe and subsequent perfusion to obtain samples cause damage in the vicinity of the insertion site, raising questions regarding the validity of the measurements.

New Method: We used fluorogenic derivatization liquid chromatography-tandem mass spectrometry, that quantifies both high and low abundance proteins, to differentiate the effects of perfusion from the effects of probe insertion on the proteomic profiles of expressed proteins in rat brain. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270183041
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http://dx.doi.org/10.1016/j.jneumeth.2018.12.020DOI Listing
March 2019
2 Reads

Perfusion alters free zinc levels in the rodent brain.

J Neurosci Methods 2019 Mar 30;315:14-16. Epub 2018 Dec 30.

Dept. Psychology, Cognitive/Behavioral Neuroscience, George Mason University, Fairfax, VA, 22030, United States. Electronic address:

Background: Fixation of brain tissue is a common practice which allows preservation of tissue and aids in preventing structural and chemical abnormalities. However, fixation procedures may disrupt the levels of biometals such as zinc when compared to tissue that is fresh-frozen. Thus, we sought to determine if any differences in free-zinc levels exist between perfused and fresh-frozen tissue. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2018.12.018DOI Listing
March 2019
1 Read

A survey on applications and analysis methods of functional magnetic resonance imaging for Alzheimer's disease.

J Neurosci Methods 2019 Apr 26;317:121-140. Epub 2018 Dec 26.

Center for Advanced Technology and Education, Department of Electrical and Computer Engineering, Florida International University, Miami, FL, United States.

Background: Functional magnetic resonance imaging (fMRI) is an MRI-based neuroimaging technique that measures brain activity on the basis of blood oxygenation level. This study reviews the main fMRI methods reported in the literature and their related applications in clinical and preclinical studies, focusing on relating functional brain networks in the prodromal stages of Alzheimer's disease (AD), with a focus on the transition phases from cognitively normal (CN) to mild cognitive impairment (MCI) and from MCI to AD.

New Method: The purpose of this study is to present and compare different approaches of supervised and unsupervised fMRI analyses and to highlight the different applications of fMRI in the diagnosis of MCI and AD. Read More

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http://dx.doi.org/10.1016/j.jneumeth.2018.12.012DOI Listing
April 2019
3 Reads

A novel motor imagery hybrid brain computer interface using EEG and functional transcranial Doppler ultrasound.

J Neurosci Methods 2019 Feb 24;313:44-53. Epub 2018 Dec 24.

Electrical and Computer Engineering, University of Pittsburgh, 3700 O'Hara St, Pittsburgh, PA 15213, USA.

Background: Hybrid brain computer interfaces (BCIs) combining multiple brain imaging modalities have been proposed recently to boost the performance of single modality BCIs.

New Method: In this paper, we propose a novel motor imagery (MI) hybrid BCI that uses electrical brain activity recorded using Electroencephalography (EEG) as well as cerebral blood flow velocity measured using functional transcranial Doppler ultrasound (fTCD). Features derived from the power spectrum for both EEG and fTCD signals were calculated. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650270183038
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http://dx.doi.org/10.1016/j.jneumeth.2018.11.017DOI Listing
February 2019
2 Reads