214 results match your criteria Journal of Negative Results in Biomedicine [Journal]


Blink rate is associated with drug-induced parkinsonism in patients with severe mental illness, but does not meet requirements to serve as a clinical test: the Curacao extrapyramidal syndromes study XIII.

J Negat Results Biomed 2017 Aug 25;16(1):15. Epub 2017 Aug 25.

Department of Psychiatry and Psychology, Maastricht University Medical Centre, South Limburg Mental Health and Teaching Network, Maastricht, The Netherlands.

Background: Drug-induced parkinsonism (DIP) has a high prevalence and is associated with poorer quality of life. To find a practical clinical tool to assess DIP in patients with severe mental illness (SMI), the association between blink rate and drug-induced parkinsonism (DIP) was assessed.

Methods: In a cohort of 204 SMI patients receiving care from the only mental health service of the previous Dutch Antilles, blink rate per minute during conversation was assessed by an additional trained movement disorder specialist. Read More

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http://dx.doi.org/10.1186/s12952-017-0079-yDOI Listing
August 2017
5 Reads

In vitro aggregating β-lactamase-polyQ chimeras do not induce toxic effects in an in vivo Caenorhabditis elegans model.

J Negat Results Biomed 2017 Aug 22;16(1):14. Epub 2017 Aug 22.

Animal Physiology and Neurobiology, Department of Biology, KU Leuven (University of Leuven), Zoological Institute, Naamsestraat 59, 3000, Leuven, Belgium.

Background: A series of human diseases are caused by the misfolding and aggregation of specific proteins or peptides into amyloid fibrils; nine of these diseases, referred to as polyglutamine diseases, are associated with proteins carrying an expanded polyglutamine (polyQ) region. While the presence of this latter is thought to be the determinant factor for the development of polyQ diseases, the non-polyQ regions of the host proteins are thought to play a significant modulating role.

Method: In order to better understand the role of non-polyQ regions, the toxic effects of model proteins bearing different polyQ regions (containing up to 79 residues) embedded at two distinct locations within the β-lactamase (BlaP) host enzyme were evaluated in Caenorhabditis elegans. Read More

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http://dx.doi.org/10.1186/s12952-017-0080-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568214PMC
August 2017
5 Reads

Work-focused cognitive behavioral intervention for psychological complaints in patients on sick leave due to work-related stress: Results from a randomized controlled trial.

J Negat Results Biomed 2017 Aug 22;16(1):13. Epub 2017 Aug 22.

Danish Ramazzini Centre, Department of Occupational Medicine, The Regional Hospital West Jutland - University Research Clinic, Herning, Denmark.

Background: Work-related stress is a global problem with negative implications for individuals and society. The purpose of the current study was to evaluate a stress management intervention for patients on sick leave due to work-related stress complaints using a three-armed randomized controlled design.

Methods: Participants were patients referred from three municipalities to the regional Department of Occupational Medicine. Read More

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http://dx.doi.org/10.1186/s12952-017-0078-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567478PMC
August 2017
9 Reads

Polymorphism rs547984 on human chromosome 1q43 is not associated with primary open angle glaucoma in a Saudi cohort.

J Negat Results Biomed 2017 Jun 26;16(1):12. Epub 2017 Jun 26.

Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.

Background: To investigate the association between polymorphism rs547984, located in close proximity to the Zona Pellucida Glycoprotein 4 (ZP4) gene on human chromosome 1q43 and primary open angle glaucoma (POAG).

Method: Polymorphism rs547984 was genotyped using Taq-Man® assay in 185 subjects comprising of 90 unrelated POAG cases and 95 controls of Saudi origin.

Results: Association analysis between cases and controls revealed no significant genotype distribution under additive (p = 0. Read More

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http://dx.doi.org/10.1186/s12952-017-0077-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483842PMC
June 2017
11 Reads

Behavioral and neural adaptations in response to five weeks of balance training in older adults: a randomized controlled trial.

J Negat Results Biomed 2017 Jun 13;16(1):11. Epub 2017 Jun 13.

Movement and Sport Sciences, Department of Medicine, University of Fribourg, Boulevard de Pérolles 90, 1700, Fribourg, Switzerland.

Background: While the positive effect of balance training on age-related impairments in postural stability is well-documented, the neural correlates of such training adaptations in older adults remain poorly understood. This study therefore aimed to shed more light on neural adaptations in response to balance training in older adults.

Methods: Postural stability as well as spinal reflex and cortical excitability was measured in older adults (65-80 years) before and after 5 weeks of balance training (n = 15) or habitual activity (n = 13). Read More

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http://dx.doi.org/10.1186/s12952-017-0076-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470269PMC
June 2017
9 Reads

Does the placebo effect modulate drug bioavailability? Randomized cross-over studies of three drugs.

J Negat Results Biomed 2017 May 23;16(1):10. Epub 2017 May 23.

Clinical Studies and Empirical Ethics Department, King Faisal Specialist Hospital and Research Center, P O Box # 3354 (MBC 03), Riyadh, 11211, Saudi Arabia.

Background: Medication effect is the sum of its drug, placebo, and drug*placebo interaction effects. It is conceivable that the interaction effect involves modulating drug bioavailability; it was previously observed that being aware of caffeine ingestion may prolong caffeine plasma half-life. This study was set to evaluate such concept using different drugs. Read More

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http://dx.doi.org/10.1186/s12952-017-0075-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442689PMC
May 2017
3 Reads

Absence of the dermatan sulfate chain of decorin does not affect mouse development.

J Negat Results Biomed 2017 Apr 17;16(1). Epub 2017 Apr 17.

Research Center, Shriners Hospitals for Children - Canada, 1003 Boulevard Décarie, Montréal, H4A 0A9, QC, Canada.

Background: In vitro studies suggest that the multiple functions of decorin are related to both its core protein and its dermatan sulfate chain. To determine the contribution of the dermatan sulfate chain to the functional properties of decorin in vivo, a mutant mouse whose decorin lacked a dermatan sulfate chain was generated.

Results: Homozygous mice expressing only the decorin core protein developed and grew in a similar manner to wild type mice. Read More

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http://dx.doi.org/10.1186/s12952-017-0074-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393030PMC
April 2017
22 Reads

The effects of phosphanegold(I) thiolates on the biological properties of Acanthamoeba castellanii belonging to the T4 genotype.

J Negat Results Biomed 2017 Apr 3;16(1). Epub 2017 Apr 3.

Department of Biological Sciences, Faculty of Science and Technology, Sunway University, 47500 Bandar Sunway, Selangor, Malaysia.

Background: Gold compounds have shown promise in the treatment of non-communicable diseases such as rheumatoid arthritis and cancer, and are considered of value as anti-microbial agents against Gram-negative and Gram-positive bacteria, and have anti-parasitic properties against Schistosoma mansoni, Trypanosoma brucei, Plasmodium falciparum, Leishmania infantinum, Giardia lamblia, and Entamoeba histolytica. They are known to affect enzymatic activities that are required for the cellular respiration processes.

Methods: Anti-amoebic effects of phosphanegold(I) thiolates were tested against clinical isolate of A. Read More

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http://dx.doi.org/10.1186/s12952-017-0070-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376697PMC
April 2017
9 Reads

Facial cutaneo-mucosal venous malformations can develop independently of mutation of TEK gene but may be associated with excessive expression of Src and p-Src.

J Negat Results Biomed 2017 Mar 20;16(1). Epub 2017 Mar 20.

Laboratory of Applied Molecular Biology and Immunology, University of Tlemcen, Imama-Mansourah, Rocade # 2, PO Box: 262, Tlemcen, 13000, Algeria.

We aimed to search for mutations in the germline and somatic DNA of the TEK gene and to analyze the expression level of Src and phospho-Src (p-Src) in tumor and healthy tissues from patients with facial cutaneo-mucosal venous malformations (VMCM). Eligible patients from twelve families and thirty healthy controls were recruited respectively at the Departments of Stomatology and Oral Surgery, and Transfusion Medicine of Tlemcen University Medical Centre. Immunoblot analyses of Src and p-Src were performed after direct DNA sequencing. Read More

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http://dx.doi.org/10.1186/s12952-017-0072-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357811PMC
March 2017
19 Reads

Experimental silicosis does not aggravate collagen-induced arthritis in mice.

J Negat Results Biomed 2017 Mar 13;16(1). Epub 2017 Mar 13.

Institute of Immunology & Core Facility for Cell Sorting & Cell Analysis, Rostock University Medical Center, Schillingallee 70, 18057, Rostock, Germany.

Objectives: To investigate the effect of chronic lung inflammation on the incidence and severity of collagen-induced arthritis in mice.

Methods: Chronic lung inflammation in the form of silicosis was induced via intranasal application of silica particles. Immunization with collagen Type II commenced one week later and mice were sacrificed six weeks after booster immunization. Read More

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http://dx.doi.org/10.1186/s12952-017-0071-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346855PMC
March 2017
3 Reads

Erratum to: Do anti-amyloid beta protein antibody cross reactivities confound Alzheimer disease research?

J Negat Results Biomed 2017 Mar 8;16(1). Epub 2017 Mar 8.

Department of Public Health and Primary Care, Institute of Public Health Forvie Site, University of Cambridge School of Clinical Medicine, Box 113 Cambridge Biomedical Campus, Cambridge, CB2 0SP, UK.

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http://jnrbm.biomedcentral.com/articles/10.1186/s12952-017-0
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http://dx.doi.org/10.1186/s12952-017-0073-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343297PMC
March 2017
3 Reads

The diagnostic value of the bronchoalveolar lavage in interstitial lung diseases.

J Negat Results Biomed 2017 Mar 1;16(1). Epub 2017 Mar 1.

Department of Pathology, Hassan II Teaching Hospital, (B.P. 1835, Atlas) Route de sidi Harazem Fès - Maroc, Fez, Morocco.

Objective: Bronchoalveolar lavage (BAL) is a diagnostic tool often used during the management of interstitial lung diseases (ILD). However, its diagnostic value in discrimination between entities comprising the very heterogenous group of ILD, is still a controversial issue. The objective of our study is to assess the diagnostic value of BAL in the management of ILD, by comparing the cytological findings in BAL fluid among the different diseases of this group. Read More

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http://dx.doi.org/10.1186/s12952-017-0069-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331687PMC
March 2017
21 Reads

Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus.

J Negat Results Biomed 2017 Feb 19;16(1). Epub 2017 Feb 19.

Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Buenteweg 17, 30599, Hannover, Germany.

Background: The human leukemia cell line HL-60 is considered an alternative cell culture model to study neutrophil differentiation and migration. The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutrophils to investigate the interaction of neutrophils with Staphylococcus aureus.

Methods: For this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen. Read More

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http://dx.doi.org/10.1186/s12952-017-0067-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316427PMC
February 2017
22 Reads

Lack of association between polymorphism rs540782 and primary open angle glaucoma in Saudi patients.

J Negat Results Biomed 2017 Feb 2;16(1). Epub 2017 Feb 2.

Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.

Background: To investigate whether polymorphism rs540782 on chromsome 1, in close proximity to the Zona Pellucida Glycoprotein 4 (ZP4) gene, is a risk factor for primary open angle glaucoma (POAG).

Method: The study genotyped 92 unrelated POAG cases and 95 control subjects from Saudi Arabia using Taq-Man® assay.

Results: The genotype frequency distribution did not deviate significantly from the Hardy-Weinberg equilibrium (p > 0. Read More

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http://dx.doi.org/10.1186/s12952-017-0068-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290620PMC
February 2017
9 Reads

Do anti-amyloid beta protein antibody cross reactivities confound Alzheimer disease research?

J Negat Results Biomed 2017 Jan 26;16(1). Epub 2017 Jan 26.

Department of Public Health and Primary Care, Institute of Public Health Forvie Site, University of Cambridge School of Clinical Medicine, Box 113 Cambridge Biomedical Campus, Cambridge, CB2 0SP, UK.

Background: Alzheimer disease (AD) research has focussed mainly on the amyloid beta protein (Aβ). However, many Aβ-and P3-type peptides derived from the amyloid precursor protein (APP) and peptides thought to derive from Aβ catabolism share sequence homology. Additionally, conformations can change dependent on aggregation state and solubility leading to significant uncertainty relating to interpretations of immunoreactivity with antibodies raised against Aβ. Read More

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http://jnrbm.biomedcentral.com/articles/10.1186/s12952-017-0
Publisher Site
http://dx.doi.org/10.1186/s12952-017-0066-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270220PMC
January 2017
6 Reads

Unspecific binding of cRNA probe to plaques in two mouse models for Alzheimer's disease.

J Negat Results Biomed 2016 Dec 16;15(1):22. Epub 2016 Dec 16.

Institute of Anatomy I, Friedrich Schiller University School of Medicine, Jena University Hospital, 07743, Jena, Germany.

Background: Alzheimer's disease (AD) is characterized by the pathological deposition of amyloid-β (Aβ) protein-containing plaques. Microglia and astrocytes are commonly attracted to the plaques by an unknown mechanism that may involve cell adhesion. One cell adhesion family of proteins, the cadherins, are widely expressed in the central nervous system. Read More

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http://dx.doi.org/10.1186/s12952-016-0065-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159973PMC
December 2016
2 Reads

False data, positive results in neurobiology: moving beyond the epigenetics of blood and saliva samples in mental disorders.

J Negat Results Biomed 2016 Dec 12;15(1):21. Epub 2016 Dec 12.

Laboratory for Neuroscience of Mental Disorders Elena Pessino, Department of Medicine and Medical Specialties, School of Medicine, Alcalá University, Madrid, Spain.

Many psychiatric diseases are influenced by a set of several genetic and environmental factors that genetics alone cannot explain. Specifically, in schizophrenia and bipolar disorder the absence of consistently replicated genetic effects together with evidence for lasting changes in gene expression after environmental exposures suggest a role of epigenetic mechanisms in its pathophysiological mechanisms. In this field, the presence of positive results could potentially uncover molecular mechanisms of deregulated gene expression in these complex disorders. Read More

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http://jnrbm.biomedcentral.com/articles/10.1186/s12952-016-0
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http://dx.doi.org/10.1186/s12952-016-0064-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5151128PMC
December 2016
4 Reads

Long-term effect of stents eluting 6-mercaptopurine in porcine coronary arteries.

J Negat Results Biomed 2016 Dec 5;15(1):20. Epub 2016 Dec 5.

Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.

Background: Drug-eluting stents (DES) have dramatically reduced restenosis rates compared to bare metal stents and are widely used in coronary artery angioplasty. The anti-proliferative nature of the drugs reduces smooth muscle cell (SMC) proliferation effectively, but unfortunately also negatively affects endothelialization of stent struts, necessitating prolonged dual anti-platelet therapy. Cell-type specific therapy may prevent this complication, giving rise to safer stents that do not require additional medication. Read More

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http://dx.doi.org/10.1186/s12952-016-0063-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137209PMC
December 2016
12 Reads

Examining if being overweight really confers protection against dementia: Sixty-four year follow-up of participants in the Glasgow University alumni cohort study.

J Negat Results Biomed 2016 Nov 2;15(1):19. Epub 2016 Nov 2.

Centre for Cognitive Ageing & Cognitive Epidemiology, University of Edinburgh, Edinburgh, UK.

Background: Recent large-scale studies suggest that obesity and overweight may confer protection against future dementia. This observation could, however, be generated by reverse causality. That is, weight loss in the incipient phase of dementia ascribed to diminished self-care, including sub-optimal nutrition, would have the effect of generating such an inverse association. Read More

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http://dx.doi.org/10.1186/s12952-016-0062-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090948PMC
November 2016
17 Reads

Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia.

J Negat Results Biomed 2016 Oct 26;15(1):18. Epub 2016 Oct 26.

Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, University of Connecticut Health, Farmington, CT, 06030, USA.

Background: Mutations in the human progressive ankylosis gene (ANKH; Mus musculus ortholog Ank) have been identified as cause for craniometaphyseal dysplasia (CMD), characterized by progressive thickening of craniofacial bones and flared metaphyses of long bones. We previously reported a knock-in (KI) mouse model (Ank ) for CMD and showed transiently lower serum phosphate (Pi) as well as significantly higher mRNA levels of fibroblast growth factor 23 (Fgf23) in Ank mice. FGF23 is secreted by bone and acts in kidney to promote Pi wasting which leads to lower serum Pi levels. Read More

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http://dx.doi.org/10.1186/s12952-016-0061-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080755PMC
October 2016
18 Reads

Polymorphism rs7555523 in transmembrane and coiled-coil domain 1 (TMCO1) is not a risk factor for primary open angle glaucoma in a Saudi cohort.

J Negat Results Biomed 2016 Sep 29;15(1):17. Epub 2016 Sep 29.

Ophthalmic Genetics Laboratory, Department of Ophthalmology, College of Medicine, King Saud University, P.O. Box 245, Riyadh, 11411, Saudi Arabia.

Background: We investigated whether polymorphism rs7555523 (A > C) in human transmembrane and coiled-coil domain 1 (TMCO1) gene is a risk factor for primary open angle glaucoma (POAG) in a Saudi cohort.

Methods: A cohort of 87 unrelated POAG cases and 94 control subjects from Saudi Arabia were genotyped using Taq-Man® assay. The association of genotypes with POAG and other glaucoma specific clinical indices was investigated. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043619PMC
http://dx.doi.org/10.1186/s12952-016-0060-1DOI Listing
September 2016
10 Reads

Low density lipoprotein receptor-related protein 5 gene polymorphisms and osteoporosis in Thai menopausal women.

J Negat Results Biomed 2016 Sep 1;15(1):16. Epub 2016 Sep 1.

Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Background: Osteoporosis, characterized by low bone mineral density (BMD) and high bone fracture risk, is prevalent in Thai menopausal women. Genetic factors are known to play a key role in BMD. Low density lipoprotein receptor-related protein 5 (LRP5), a co-receptor in the Wnt/beta-catenin pathway, is involved in many aspects of bone biology. Read More

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http://dx.doi.org/10.1186/s12952-016-0059-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007848PMC
September 2016
5 Reads

Evaluation of a novel virtual screening strategy using receptor decoy binding sites.

J Negat Results Biomed 2016 Aug 23;15(1):15. Epub 2016 Aug 23.

Department of Biological and Environmental Sciences, University of Hertfordshire, College Lane, Hatfield, AL10 9AB, UK.

Virtual screening is used in biomedical research to predict the binding affinity of a large set of small organic molecules to protein receptor targets. This report shows the development and evaluation of a novel yet straightforward attempt to improve this ranking in receptor-based molecular docking using a receptor-decoy strategy. This strategy includes defining a decoy binding site on the receptor and adjusting the ranking of the true binding-site virtual screen based on the decoy-site screen. Read More

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http://dx.doi.org/10.1186/s12952-016-0058-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994241PMC
August 2016
2 Reads

Gastrokine 1 mRNA in human sera is not informative biomarker for gastric cancer.

J Negat Results Biomed 2016 Jul 25;15(1):14. Epub 2016 Jul 25.

Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, I-8031, Naples, Italy.

Background: We aimed to ascertain if Gastrokine 1 mRNA in the sera of patients with gastric cancer might be an informative biomarker for the disease.

Results: Analysis of GKN1 mRNA in serum samples from healthy individuals (n = 23) and from patients with diagnosis of gastric cancer (n = 16), performed by using absolute quantification based on standard curve method, did not show any significative statistical difference between the two unpaired group of individuals.

Conclusions: Our preliminary results did not confirm GKN1 as a potential biomarker for gastric cancer. Read More

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http://dx.doi.org/10.1186/s12952-016-0057-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959057PMC
July 2016
8 Reads

Is mindfulness protective against PTSD? A neurocognitive study of 25 Tsunami disaster survivors.

J Negat Results Biomed 2016 Jul 20;15(1):13. Epub 2016 Jul 20.

Department of mental health and addiction, University of Oslo, Oslo, Norway.

Background: It has been suggested that mindfulness is a protective factor that buffers individuals from experiencing severe posttraumatic stress following exposure to a trauma. We aimed to examine the association between dispositional (trait) mindfulness and posttraumatic stress in individuals who had been exposed to the trauma of a natural disaster.

Method: A disaster group (n = 25) consisting of Norwegian tourists who survived the 2004 South East Asian tsunami at a location with high mortality rates was recruited. Read More

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http://dx.doi.org/10.1186/s12952-016-0056-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4952228PMC
July 2016
2 Reads

Age is not associated with intracranial haemorrhage in patients with mild traumatic brain injury and oral anticoagulation.

J Negat Results Biomed 2016 Jun 1;15(1):12. Epub 2016 Jun 1.

Department of Emergency Medicine, Inselspital, University Hospital Bern, Freiburgstrasse, 3010, Bern, Switzerland.

Background: Patients admitted to emergency departments with traumatic brain injury (TBI) are commonly being treated with oral anticoagulants. In contrast to patients without anticoagulant medication, no guidelines, scores or recommendations exist for the management of mild traumatic brain injury in these patients. We therefore tested whether age as one of the high risk factors of the Canadian head CT rule is applicable to a patient population on oral anticoagulants. Read More

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http://dx.doi.org/10.1186/s12952-016-0055-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940966PMC
June 2016
22 Reads

Investigation of SLA4A3 as a candidate gene for human retinal disease.

J Negat Results Biomed 2016 May 23;15:11. Epub 2016 May 23.

Kennel Club Genetics Centre, Animal Health Trust, Newmarket, UK.

SLC4A3 has been shown to cause retinal degeneration in a genetically engineered knockout mouse, and in a naturally occurring form of canine progressive retinal atrophy considered to be the equivalent of retinitis pigmentosa in humans (RP). This study was undertaken to investigate if SLC4A3 coding variants were implicated in human retinal degeneration. SLC4A3 exons were amplified and sequenced in 200 patients with autosomal recessive retinal degeneration who had no known molecular diagnosis for their condition, which included 197 unrelated individuals with suspected RP and three individuals with other forms of retinal disease. Read More

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http://dx.doi.org/10.1186/s12952-016-0054-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876561PMC
May 2016
10 Reads

Noise trauma and systemic application of the selective glucocorticoid receptor modulator compound A.

J Negat Results Biomed 2016 May 11;15:10. Epub 2016 May 11.

Department of Otorhinolaryngology, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.

Background: Selective glucocorticoid receptor modulators (SEGRMs) comprise a novel class of drugs promising both reduced side effects and similar pharmacological potency relative to glucocorticoids, which presently serve as the only clinical treatment for many otologic disorders. In the first otologic SEGRM experiment in an animal model of noise trauma, we compare the effects of Compound A (a SEGRM) and dexamethasone (potent glucocorticoid).

Methods: Forty adult guinea pigs received experimental treatment once daily for ten days. Read More

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http://dx.doi.org/10.1186/s12952-016-0053-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863352PMC
May 2016
3 Reads

Intracerebroventricular injections of dronabinol, a cannabinoid receptor agonist, does not attenuate serotonin-induced apnea in Sprague-Dawley rats.

J Negat Results Biomed 2016 May 2;15. Epub 2016 May 2.

Center for Narcolepsy, Sleep and Health Research, University of Illinois at Chicago, 845 South Damen Avenue (M/C 802), Chicago, IL, 60612, USA.

Background: Evidence suggests that vagal nerve activity may play a role in sleep apnea induction. In anesthetized rats, dronabinol, a cannabinoid (CB) receptor agonist, injected into the nodose ganglia attenuates reflex apnea and increases genioglossus activity, and reflex apnea attenuation is blocked by systemic pre-treatment with cannabinoid type 1 and/or type 2 receptor antagonists. However, it is unclear whether dronabinol has similar effects in the central nervous system; CB receptors are widely distributed in the brain, especially on neuronal circuitry important for respiration and upper airway activation. Read More

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http://dx.doi.org/10.1186/s12952-016-0052-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852437PMC
May 2016
7 Reads

Tlr2 deficiency does not limit the development of left ventricular hypertrophy in a model of transverse aortic constriction induced pressure overload.

J Negat Results Biomed 2016 Apr 25;15. Epub 2016 Apr 25.

Department of Anaesthesiology and Intensive Care Medicine, University Hospital Bonn, Sigmund-Freud-Straße 25, 53127, Bonn, Germany.

Background: Toll-like receptors (TLRs) are involved in a variety of cardiovascular disorders, including septic cardiomyopathy, ischemia/reperfusion, heart failure, and cardiac hypertrophy. Previous research revealed that TLR4 promotes cardiac hypertrophy in vivo. Therefore, we investigated whether TLR2 is also involved in the development of cardiac hypertrophy. Read More

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http://dx.doi.org/10.1186/s12952-016-0050-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4843197PMC
April 2016
33 Reads

Endothelial, platelet, and macrophage microparticle levels do not change acutely following transcatheter aortic valve replacement.

J Negat Results Biomed 2016 Apr 11;15. Epub 2016 Apr 11.

Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Background: Patients with severe aortic stenosis have increased levels of prothrombotic and proinflammatory microparticles (MP), and MPs actively regulate pathological processes that lead to atherothrombotic cardiovascular events. Shear stress is a validated stimulus of MP production, and abnormal shear stress in aortic stenosis increases MP release in ex-vivo studies. We hypothesized that in patients with severe aortic stenosis, percutaneous replacement of the aortic valve (TAVR) would reduce abnormal shear stress and would decrease levels of circulating MPs. Read More

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http://dx.doi.org/10.1186/s12952-016-0051-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827212PMC
April 2016
15 Reads

Targeted drugs and Psycho-oncological intervention for breast cancer patients.

J Negat Results Biomed 2016 Apr 1;15. Epub 2016 Apr 1.

Department of Environment and Primary Prevention, Italian National Institute of Health, ISS, V.le Regina Elena 299, 00161, Rome, Italy.

Personalized medicine is a new field based on molecular biology and genomics in which targeted tumor therapies are administered to patients. Psycho-oncology is a complementary approach that considers social and psychological aspects of patients as part of the treatments for cancer patients. The aim of this mini-review is to weigh clinical benefits for breast cancer patients of both treatments and possibly enhance benefits by modulating the use of both interventions. Read More

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http://dx.doi.org/10.1186/s12952-016-0049-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818528PMC
April 2016
3 Reads

Topical ocular dexamethasone decreases intraocular pressure and body weight in rats.

J Negat Results Biomed 2016 Mar 12;15. Epub 2016 Mar 12.

Department of Ophthalmology, Tohoku University Graduate School of Medicine, 1-1, Seiryo, Aoba, Sendai, Miyagi, 980-8574, Japan.

Background: Recently, topical dexamethasone-induced ocular hypertension and a consequent loss of retinal ganglion cells (RGCs) have been described in mice. This has been proposed as a model of steroid-induced glaucoma. In this study, we set up and evaluated a similar model in rats. Read More

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http://dx.doi.org/10.1186/s12952-016-0048-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4789283PMC
March 2016
15 Reads

Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria.

J Negat Results Biomed 2016 Mar 2;15. Epub 2016 Mar 2.

Grupo Investigador de Problemas en Enfermedades Infecciosas (GRIPE), Sede de Investigación Universitaria, Calle 62 # 52-59, Laboratorio 630, Universidad de Antioquia, Medellín, Colombia.

Background: The diagnosis of community-acquired pneumonia (CAP) caused by Legionella pneumophila, Mycoplasma pneumoniae, and Chlamydophila pneumoniae is traditionally based on cultures and serology, which have special requirements, are time-consuming, and offer delayed results that limit their clinical usefulness of these techniques. We sought to develop a multiplex PCR (mPCR) method to diagnosis these bacterial infections in CAP patients and to compare the diagnostic yields obtained from mPCR of nasopharyngeal aspirates (NPAs), nasopharyngeal swabs (NPSs), and induced sputum (IS) with those obtained with specific PCR commercial kits, paired serology, and urinary antigen.

Results: A total of 225 persons were included. Read More

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http://dx.doi.org/10.1186/s12952-016-0047-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774004PMC
March 2016
8 Reads

ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model.

J Negat Results Biomed 2016 Feb 3;15. Epub 2016 Feb 3.

Key Laboratory of Brain Functional Genomics (East China Normal University), Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics (East China Normal University), School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai, 200062, China.

Background: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene.

Results: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Read More

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http://dx.doi.org/10.1186/s12952-016-0045-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739331PMC
February 2016
35 Reads

Use of Diuretics is not associated with mortality in patients admitted to the emergency department: results from a cross-sectional study.

J Negat Results Biomed 2016 Feb 1;15. Epub 2016 Feb 1.

Department of Emergency Medicine, Inselspital, University Hospital Bern, Freiburgstrasse, Bern, 3010, Switzerland.

Background: Patients with diuretic therapy are at risk for drug-induced adverse reactions. It is unknown if presence of diuretic therapy at hospital emergency room admission is associated with mortality.

Methods: In this cross sectional analysis, all emergency room patients 2010 and 2011 at the Inselspital Bern, Switzerland were included. Read More

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http://dx.doi.org/10.1186/s12952-016-0044-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736646PMC
February 2016
17 Reads

Mobile phone use and risk for intracranial tumors.

J Negat Results Biomed 2015 Dec 23;14:23. Epub 2015 Dec 23.

Neurosurgical Institute, University of Ioannina, Po box 103, Neohoropoulo, Ioannina, 45500, Greece.

Mobile phone use has been discussed over the last few decades with increased risk for intracranial tumors. The majority of studies have been conducted on gliomas and meningiomas. Although some case-control studies have found a positive association between the use of mobile phones and the risk of tumors, other studies have reported no significant association. Read More

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http://dx.doi.org/10.1186/s12952-015-0043-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690283PMC
December 2015
10 Reads

Substrate stiffness effect and chromosome missegregation in hIPS cells.

J Negat Results Biomed 2015 Dec 18;14:22. Epub 2015 Dec 18.

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA.

Background: Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful cell therapies. Although most instances lead to failed experiments and go unreported in the literature, many laboratories have found the emergence of genetic abnormalities in PSCs when cultured in vitro for prolonged amounts of time. These cells are primarily cultured in non-physiological stiff substrates like tissue culture polystyrene (TCPS) which raises the possibility that the cause of these abnormalities may be influenced by substrate mechanics. Read More

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http://dx.doi.org/10.1186/s12952-015-0042-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683860PMC
December 2015
5 Reads

Effects of omega-3 fatty acids on arterial stiffness in patients with hypertension: a randomized pilot study.

J Negat Results Biomed 2015 Dec 2;14:21. Epub 2015 Dec 2.

Cardiology Division, Denver Health Medical Center, 777 Bannock St., MC0960, Denver, CO, 80204, USA.

Background: Omega-3 fatty acids prevent cardiovascular disease (CVD) events in patients with myocardial infarction or heart failure. Benefits in patients without overt CVD have not been demonstrated, though most studies did not use treatment doses (3.36 g) of omega-3 fatty acids. Read More

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http://dx.doi.org/10.1186/s12952-015-0040-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668614PMC
December 2015
16 Reads

Ethanol exposure during the third trimester equivalent does not affect GABAA or AMPA receptor-mediated spontaneous synaptic transmission in rat CA3 pyramidal neurons.

J Negat Results Biomed 2015 Dec 2;14:19. Epub 2015 Dec 2.

Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA.

Background: Ethanol exposure during the rodent equivalent to the 3(rd) trimester of human pregnancy (i.e., first 1-2 weeks of neonatal life) has been shown to produce structural and functional alterations in the CA3 hippocampal sub-region, which is involved in associative memory. Read More

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http://dx.doi.org/10.1186/s12952-015-0041-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667401PMC
December 2015
2 Reads

The rs361525 polymorphism does not increase production of tumor necrosis factor alpha by monocytes from alpha-1 antitrypsin deficient subjects with chronic obstructive pulmonary disease - a pilot study.

J Negat Results Biomed 2015 Dec 1;14:20. Epub 2015 Dec 1.

Institute of Inflammation and Ageing, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.

Background: Polymorphisms in the TNF-A gene have been associated with chronic obstructive pulmonary disease (COPD) in some case-control studies. Previous work has shown that COPD/chronic bronchitis subjects with alpha-1 antitrypsin deficiency with the rs361525 TNF-α single nucleotide polymorphism have 100 times more TNF-in spontaneous sputum than disease matched controls. Our objective was to determine if the presence of this polymorphism increased TNF-α production by blood monocytes from COPD subjects. Read More

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http://dx.doi.org/10.1186/s12952-015-0039-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665865PMC
December 2015
10 Reads

Early objective response may not be a prognostic factor of survival for patients with metastatic urothelial carcinoma: from a retrospective analysis of a cohort of 113 patients.

J Negat Results Biomed 2015 Nov 10;14:18. Epub 2015 Nov 10.

Department of Medical Oncology, Institut Bergonié, 229, Cours de l'Argonne, 33076, Bordeaux, France.

Background: This study aims to better define prognostic factors for patients with metastatic urothelial carcinoma (mUC), and to identify patients who will benefit from first-line cisplatin-based chemotherapy. We test the hypothesis that early objective response (EOR), defined as the occurrence of an objective response following 2 or 3 courses of chemotherapy, could be a prognostic factor for overall survival (OS) and thus be used to guide treatment decisions. Data from 113 patients with evaluable mUC receiving first-line cisplatin-based treatment between January 2004 and December 2006 was collected retrospectively from prospectively-maintained databases across seven French cancer centers. Read More

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http://dx.doi.org/10.1186/s12952-015-0037-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641378PMC
November 2015
25 Reads

Willis-Ekbom disease is not associated with poor cardiovascular health in adults.

J Negat Results Biomed 2015 Nov 6;14:17. Epub 2015 Nov 6.

Division of Pulmonary and Allergy Medicine, Mayo Clinic, 4500 San Pablo Rd, Jacksonville, FL, 32224, USA.

Background: Willis-Ekbom disease (WED), also called restless legs syndrome (RLS), is a neurologic sensorimotor disease that may be associated with cardiovascular disease. Given high morbidity and mortality rates of cardiovascular disease worldwide, we assessed the relation between WED/RLS and cardiovascular health risks in a native South American population. We prospectively analyzed data from The Atahualpa Project of Ecuadorian adults aged 40 years and older. Read More

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http://dx.doi.org/10.1186/s12952-015-0038-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636766PMC
November 2015
5 Reads

MicroRNAs in urine are not biomarkers of multiple myeloma.

J Negat Results Biomed 2015 Sep 23;14:16. Epub 2015 Sep 23.

Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.

Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0. Read More

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http://dx.doi.org/10.1186/s12952-015-0035-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581458PMC
September 2015
38 Reads

A double-blind, randomised, placebo-controlled study of roxithromycin and doxycycline combination, roxithromycin alone, or matching placebo for 12 weeks in adults with frequent exacerbations of chronic obstructive pulmonary disease.

J Negat Results Biomed 2015 Sep 7;14:15. Epub 2015 Sep 7.

Faculty of Medical and Health Sciences, The University of Auckland and Auckland Hospital, Auckland, New Zealand.

Background: Azithromycin prophylaxis has been shown to reduce COPD exacerbations but there is poor evidence for other antibiotics. We compared exacerbation rates in COPD patients with a history of frequent exacerbations (at least three moderate or severe COPD exacerbations in the past two years) during a 12-week treatment course and over a subsequent 48-week follow up period.

Results: 292 patients were randomised to one of three treatments for 12 weeks: roxithromycin 300 mg daily and doxycycline 100 mg daily (n = 101); roxithromycin 300 mg daily (n = 97); or matching placebos (n = 94). Read More

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http://dx.doi.org/10.1186/s12952-015-0034-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562194PMC
September 2015
12 Reads

Maternal exercise before and during pregnancy does not impact offspring exercise or body composition in mice.

J Negat Results Biomed 2015 Aug 3;14:13. Epub 2015 Aug 3.

Department of Genetics, University of North Carolina, Chapel Hill, NC, 27599-7264, USA.

Background: The genome, the environment, and their interactions simultaneously regulate complex traits such as body composition and voluntary exercise levels. One such environmental influence is the maternal milieu (i.e. Read More

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http://dx.doi.org/10.1186/s12952-015-0032-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522962PMC
August 2015
21 Reads

Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation.

J Negat Results Biomed 2015 Jul 18;14:14. Epub 2015 Jul 18.

Department of Physiological Sciences, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.

Background: Neuroinflammation is central to the aetiology of HIV-associated neurocognitive disorders (HAND) that are prevalent in late stage AIDS. Anti-retroviral (ARV) treatments are rolled out relatively late in the context of neuroinflammatory changes, so that their usefulness in directly preventing HAND is probably limited. It is common practice for HIV+ individuals in developing countries to make use of traditional medicines. Read More

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http://jnrbm.biomedcentral.com/articles/10.1186/s12952-015-0
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http://dx.doi.org/10.1186/s12952-015-0031-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506629PMC
July 2015
4 Reads

Negative results: negative perceptions limit their potential for increasing reproducibility.

J Negat Results Biomed 2015 Jul 7;14:12. Epub 2015 Jul 7.

, P.O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan.

Negative results are an important building block in the development of scientific thought, primarily because most likely the vast majority of data is negative, i.e., there is not a favorable outcome. Read More

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http://dx.doi.org/10.1186/s12952-015-0033-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494691PMC
July 2015
2 Reads

A systematic examination of brain volumetric abnormalities in recent-onset schizophrenia using voxel-based, surface-based and region-of-interest-based morphometric analyses.

J Negat Results Biomed 2015 Jun 12;14:11. Epub 2015 Jun 12.

Department of Psychiatry, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, Karnataka, India.

Background: Brain morphometric abnormalities in schizophrenia have been extensively reported in the literature. Whole-brain volumetric reductions are almost universally reported by most studies irrespective of the characteristics of the samples studied (e.g. Read More

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http://dx.doi.org/10.1186/s12952-015-0030-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464994PMC
June 2015
26 Reads

Case-control association between CCT-associated variants and keratoconus in a Saudi Arabian population.

J Negat Results Biomed 2015 Jun 4;14:10. Epub 2015 Jun 4.

Department of Cellular Biology and Anatomy, The Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.

Background: Keratoconus (KC) is the most common primary ectatic disease of the cornea and a major indication for corneal transplant. To date, limited KC-associated-risk loci have been identified. Association has recently been suggested between KC and 8 single nucleotide polymorphisms (SNPs) in the genomic regions of FNDC3B, COL4A3, MPDZ-NF1B, RXRA-COL5A1, LCN12-PTGDS, FOXO1, and BANP-ZNF469. Read More

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http://dx.doi.org/10.1186/s12952-015-0029-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461978PMC
June 2015
13 Reads