8,408 results match your criteria Journal of Medical Virology[Journal]


Prevalence and characteristics of acute respiratory virus infections in pediatric cancer patients.

J Med Virol 2019 Feb 14. Epub 2019 Feb 14.

Department of Experimental Pathology, Immunology and Microbiology.

Background: Pediatric cancer patients have a higher risk of morbidity and mortality due to respiratory viral infections than do other patient populations.

Objectives: To investigate the causative viruses of respiratory infections and their burden among pediatric cancer patients in Lebanon.

Study Design: Nasopharyngeal swabs along with clinical and demographic data were collected from pediatric cancer patients presenting febrile episodes with upper respiratory tract symptoms. Read More

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http://doi.wiley.com/10.1002/jmv.25432
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http://dx.doi.org/10.1002/jmv.25432DOI Listing
February 2019
1 Read

Hepatitis B virus Surface gene mutants in immunoprophylaxis-failed infants from Southern China.

J Med Virol 2019 Feb 13. Epub 2019 Feb 13.

Department of Laboratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Hepatitis B virus (HBV) vaccination was considered the most powerful tool for preventation of HBV transmission from mother to infant. In 1992 a universal HBV vaccination program for infants was lauched in China. The aim of this study was to investigate the HBV Surface antigen (HBsAg) varations in immunoprophylaxis-failed infants, vaccine free infant patients and adult chronic hepatitis B(CHB) patients (without nucleoside analog treatment). Read More

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http://dx.doi.org/10.1002/jmv.25430DOI Listing
February 2019

Measurement of serum hepatitis B surface antibody (HBs-Ab) levels in Iranian autistic children and evaluation of immunological memory after booster dose injection in comparison with controls.

J Med Virol 2019 Feb 13. Epub 2019 Feb 13.

Department of Virology, school of public health, Tehran University of Medical Science, Tehran, Iran.

Background: Responsiveness to hepatitis B vaccine among Autism Spectrum Disorders (ASD) patients has not been evaluated worldwide. We aimed to determine the anti-HBs antibody duration in autistic and healthy children few years after primary vaccination and evaluate their immunological memory against Hepatitis B virus (HBV( vaccine with booster dose administration.

Methods: 107 and 147 HBsAg-negative children from ASD and normal population were recruited, respectively. Read More

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http://dx.doi.org/10.1002/jmv.25429DOI Listing
February 2019

A 3-year long investigation of the authenticity of the return results of HBV qualitative testing in EQA in East China.

J Med Virol 2019 Feb 13. Epub 2019 Feb 13.

Zhejiang Center for Clinical Laboratories, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Background: In external quality assessments (EQA), it is important and necessary for participants to return authentic test results. However, to obtain better evaluation results, some laboratories have lacked confidence that their own and have preferred to rely upon the test results in surrounding laboratories. These violations covered up errors during testing and affected the authenticity of the returned data. Read More

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http://dx.doi.org/10.1002/jmv.25431DOI Listing
February 2019

Frequency and Implications of Myeloid Derived Suppressor Cells and Lymphocyte Subsets in Egyptian Patients with Hepatitis C Virus-Related Hepatocellular Carcinoma.

J Med Virol 2019 Feb 13. Epub 2019 Feb 13.

Department of Internal Medicine, Faculty of Medicine, Assiut University, Egypt.

Background And Aim: Myeloid-derived suppressor cells (MDSCs) play a pivotal role in tumor immunity and induction of immune tolerance to a variety of antitumor effectors, including T-lymphocytes. Herein, we tried to evaluate the frequency and clinical significance of MDSCs and different lymphocyte subsets in HCV- related hepatocellular carcinoma (HCC).

Methods: Four groups were enrolled; chronic HCV (CHC, n=40), HCV related liver cirrhosis (HCV-LC, n= 40), HCV related HCC (HCV-HCC, n=75) and healthy control group (n=20). Read More

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http://dx.doi.org/10.1002/jmv.25428DOI Listing
February 2019

Spontaneously-resolving episodes of Cytomegalovirus DNAemia in allogeneic hematopoietic stem cell transplant recipients: virological features and clinical outcomes.

J Med Virol 2019 Feb 8. Epub 2019 Feb 8.

Microbiology Service, Hospital Clínico Universitario, INCLIVA Research Institute, Valencia, Spain.

It has been reported that low plasma cytomegalovirus (CMV) DNA loads are associated with an increased risk of overall mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT). Utilizing a conservative strategy for initiation of preemptive antiviral therapy (PET) (>1,500 IU/ml), we characterized the virological features of spontaneously-resolving episodes of CMV DNAemia and assessed their impact on mortality through the first year after transplantation. We reviewed the CMV DNA PCR results and clinical charts of 230 consecutive adult patients who underwent T-cell replete allo-HSCT at our center. Read More

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http://dx.doi.org/10.1002/jmv.25426DOI Listing
February 2019
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Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assays for rhinovirus detection.

J Med Virol 2019 Feb 8. Epub 2019 Feb 8.

Influenza Virus Research Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimuyayama-shi, Tokyo, Japan.

Human rhinoviruses (RVs) belong to the genus Enterovirus of the family Picornaviridae, and are classified into RV-A, -B, and -C species. Two assays were developed to detect RVs by a real-time fluorescent reverse transcription loop-mediated isothermal amplification method: one was designed based on the 5'-untranslated regions (UTRs) of RV-A and B, and the other was designed based on the 5'-UTR of RV-C. The competence of both assays for the diagnosis of RV infection was tested using isolated viruses and compared with real-time RT-PCR assays on clinical specimens. Read More

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http://dx.doi.org/10.1002/jmv.25427DOI Listing
February 2019
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Molecular epidemiology and host genetics of norovirus and rotavirus infections in Portuguese elderly living in aged care homes.

J Med Virol 2019 Feb 8. Epub 2019 Feb 8.

Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa (UNL), Rua da Junqueira 100, Portugal.

Norovirus (NoV) and rotavirus group A (RVA) are major agents of acute gastroenteritis worldwide. This study aimed to investigate their epidemiological profile in Portuguese elderly living in long-term care facilities and to assess the host genetic factors mediating infection susceptibility. From November 2013 to June 2015, 636 faecal specimens from 169 elderly, mainly asymptomatic, living in nursing homes in Greater Lisbon and Faro district, Portugal, were collected. Read More

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http://dx.doi.org/10.1002/jmv.25425DOI Listing
February 2019

Detection of chikungunya virus-specific IgM on laser-cut paper-based device using pseudo-particles as capture antigen.

J Med Virol 2019 Feb 8. Epub 2019 Feb 8.

bioMérieux, Innovations New Immuno-Concepts department, Chemin de l'Orme, Marcy-l'Etoile, France.

The incidence of arbovirus infections has increased dramatically in recent decades, affecting hundreds of millions of people each year. The Togaviridae family includes the chikungunya virus (CHIKV), which is typically transmitted by Aedes mosquitoes and causes a wide range of symptoms from flu-like fever to severe arthralgia. Although conventional diagnostic tests can provide early diagnosis of CHIKV infections, access to these tests is often limited in developing countries. Read More

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http://dx.doi.org/10.1002/jmv.25420DOI Listing
February 2019

Human platelet antigens polymorphisms; Association to the development of liver fibrosis in patients with chronic hepatitis C.

J Med Virol 2019 Feb 6. Epub 2019 Feb 6.

Blood Transfusion Research Center /High Institute for Research & Education in Transfusion Medicine, Tehran Blood Transfusion Center.

Recently, human platelet antigens (HPAs) polymorphisms are found to play a role in susceptibility to HCV infection and fibrosis progression. The aim of the current study was to evaluate the possible association between HPAs polymorphisms with liver fibrosis progression in HCV patients. HPAs polymorphisms genotyping was performed in HCV patients (n=71) by SSP-PCR. Read More

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http://dx.doi.org/10.1002/jmv.25423DOI Listing
February 2019
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MERS-CoV, influenza and other respiratory viruses among symptomatic pilgrims during 2014 Hajj season.

J Med Virol 2019 Feb 6. Epub 2019 Feb 6.

Special Infectious Agent Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.

More than two million Muslims visit Makkah, Saudi Arabia, annually to perform the religious rituals of Hajj where the risk of spreading respiratory infections is very common. The aim here was to screen symptomatic pilgrims for Middle East respiratory syndrome-Coronavirus (MERS-CoV) and other viral etiologies. Thus, 132 nasopharyngeal samples were collected from pilgrims presenting with acute respiratory symptoms at the healthcare facilities in the holy sites during the five-days of the 2014 Hajj season. Read More

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http://dx.doi.org/10.1002/jmv.25424DOI Listing
February 2019
2.347 Impact Factor

Th17 serum cytokines in relation to laboratory-confirmed respiratory viral infection: A pilot study.

J Med Virol 2019 Feb 4. Epub 2019 Feb 4.

4th Department of Internal Medicine, University of Athens Medical School, Athens, Greece.

Background: Th17 cytokines are associated with modulation of inflammation and may be beneficial in clearing influenza infection in experimental models. The Th17 cytokine profile was evaluated in a pilot study of respiratory virus infections.

Methods: Consecutive patients with symptoms of respiratory tract infection visiting the emergency department of a tertiary care hospital during the winter influenza season of 2014 to 2015 were evaluated. Read More

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http://dx.doi.org/10.1002/jmv.25406DOI Listing
February 2019
3 Reads
2.347 Impact Factor

A fluorescence method for homogeneous detection of influenza A (H1N1) DNA sequence based on Guanine(G)-quadruplex- N-methylmesoporphyrin IX (NMM) complex and Assistance-DNA (A-DNA) inhibition.

J Med Virol 2019 Feb 4. Epub 2019 Feb 4.

School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, P. R. China.

In this work, we report a fluorescence method for homogeneous detection of influenza A (H1N1) DNA sequence based on G-quadruplex-NMM complex and Assistance DNA (A-DNA) inhibition. The quadruplex-based functional DNA (QBF-DNA), composed of complementary probe to the target H1N1 DNA sequence and G-rich fragment, was designed as the signal DNA. The A-DNA consisted of two parts, one part was complementary to target H1N1 DNA and the other part was complementary to the signal DNA. Read More

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http://dx.doi.org/10.1002/jmv.25422DOI Listing
February 2019

Analysis of HBV X gene quasispecies characteristics by next-generation sequencing and cloning-based sequencing and its association with hepatocellular carcinoma progression.

J Med Virol 2019 Feb 3. Epub 2019 Feb 3.

Experimental Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Objectives: This study aimed to describe the differences between next-generation sequencing (NGS) and cloning-based sequencing (CBS) in HBX quasispecies research and primitively investigate the relationship between the dominant HBX quasispecies and hepatocellular carcinoma (HCC).

Methods: A total of 12 serum samples were collected. Serum hepatitis B virus (HBV) DNA was extracted, and the HBV X-region (HBX) was amplified by nested polymerase chain reaction (PCR). Read More

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http://dx.doi.org/10.1002/jmv.25421DOI Listing
February 2019

Imbalance in the ratio of CpG and polyG contributes to impaired interferon-α expression.

J Med Virol 2019 Jan 31. Epub 2019 Jan 31.

Department of Microbiology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.

The secretion of interferon-α (IFN-α) is impaired during hepatitis B virus (HBV) infection. DNA sequences purified from distinct viruses, for example, HBV versus members of Herpesviridae, have been shown to differ in their IFN-α signaling properties. The present study found that DNA from HBV inhibited, while DNA from members of Herpesviridae induced, the expression of IFN-α. Read More

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http://doi.wiley.com/10.1002/jmv.25419
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http://dx.doi.org/10.1002/jmv.25419DOI Listing
January 2019
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Transgenic increase in the β-endorphin concentration in cerebrospinal fluid alleviates morphine-primed relapse behavior through the μ opioid receptor in rats.

J Med Virol 2019 Jan 31. Epub 2019 Jan 31.

Department of Anesthesiology & Intensive Care, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.

Background: Opioid-primed relapse is a global burden. Although current strategies have improved, optimal therapy is urgently needed.

Methods: A recombinant adenovirus (Ad-NEP) expressing β-endorphin (β-EP) was designed and injected intracerebroventricularly (i. Read More

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http://dx.doi.org/10.1002/jmv.25415DOI Listing
January 2019

Hollow Mesoporous Silica Nanoparticles as Delivery Vehicle of Foot-and-Mouth Disease Virus-like Particles Induce Persistent Immune Responses in Guinea Pigs.

J Med Virol 2019 Jan 30. Epub 2019 Jan 30.

State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, P. R. China.

Foot-and-mouth disease (FMD) is an acute and febrile infectious disease, which can cause great economic losses. Virus-like particles (VLPs) as an advantageous antigen can induce significant specific immune response. In order to improve immunity of VLPs, especially, make it induce persistent immune response, the hollow mesoporous silica nanoparticles (HMSNs) as a potential nano-adjuvant were synthesized and loaded the FMDV VLPs. Read More

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http://dx.doi.org/10.1002/jmv.25417DOI Listing
January 2019

Dengue virus serotypes and genotypic characterization from northeast India.

J Med Virol 2019 Jan 30. Epub 2019 Jan 30.

Office of District Medical and Health Officer, Tura, Meghalaya, India.

Dengue is a rapidly spreading acute arboviral infection transmitted through a human and Aedes mosquito cycle. Though northeast region of India has been experiencing dengue outbreaks regularly for over a decade, reports on genetic characterization of the virus from this region are limited. The present study was undertaken to detect the genotype and genetic composition of circulating dengue virus (DENV) in this region. Read More

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http://dx.doi.org/10.1002/jmv.25418DOI Listing
January 2019
3 Reads

The association between consuming bivalves, and acute gastroenteritis and norovirus in Tokyo, Japan.

J Med Virol 2019 Jan 30. Epub 2019 Jan 30.

Department of Virology, Tohoku University Graduate School of Medicine, Sendai, Japan.

A prospective matched case-control study was conducted to evaluate associations between dietary histories, including consumption of bivalves, diarrhea, and norovirus positive diarrhea in adult ambulatory patients at an outpatient clinic of a hospital in Tokyo, Japan. Ambulatory cases with diarrhea were matched with nondiarrheal control patients, who visited the same clinic. A standardized questionnaire was used to obtain patients' information, including histories of food consumption and clinical information. Read More

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http://dx.doi.org/10.1002/jmv.25416DOI Listing
January 2019
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The Role of Lipid Rafts in Cell Entry of Human Metapneumovirus.

J Med Virol 2019 Jan 30. Epub 2019 Jan 30.

Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation base of Child development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.

Human metapneumovirus (hMPV) is a crucial pathogen in children. Cell entry is the first step for infection. Our previous study indicated that there was an endocytosis pathway for hMPV cell entry. Read More

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http://dx.doi.org/10.1002/jmv.25414DOI Listing
January 2019
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Clinical outcomes after the introduction of direct antiviral agents for patients infected with genotype 1b hepatitis C virus depending on the regimens: A multicenter study in Korea.

J Med Virol 2019 Jan 29. Epub 2019 Jan 29.

Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Background: A real-life study is essential outside clinical trials. The aim is to evaluate the clinical outcomes of direct acting agents (DAA) for patients with chronic hepatitis C (CHC) in real practice.

Methods: We analyzed 590 consecutively enrolled patients with CHC-1b who received DAAs since 2015, when DAAs were introduced in Korea. Read More

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http://dx.doi.org/10.1002/jmv.25412DOI Listing
January 2019
2.347 Impact Factor

Non-F HBV/HDV-3 coinfection is associated with severe liver disease in Western Brazilian Amazon.

J Med Virol 2019 Jan 29. Epub 2019 Jan 29.

Institut National de la Santé et de la Recherche Medicale, INSERM U1052, Lyon, France.

The clinical outcome of hepatitis B virus (HBV) infection may be related to host and viral genetic factors, as well as to the type of infection (monoinfection and coinfection). To analyze the distribution/combination of HBV/hepatitis D virus (HDV) genotypes and the associated clinical characteristics, 409 serum samples from patients with chronic HBV (94 of them coinfected by HDV) followed at the Viral Hepatitis Referral Center of Rio Branco, Brazil were enrolled. HBV DNA and HDV RNA were amplified, respectively, by polymerase chain reaction (PCR) and nested PCR using specific primers in the PreC/C region and the S gene, and by reverse-transcription PCR and seminested PCR using specific primers in the delta antigen region and sequenced. Read More

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http://doi.wiley.com/10.1002/jmv.25411
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http://dx.doi.org/10.1002/jmv.25411DOI Listing
January 2019
3 Reads

Viral evolution in the cell-associated HIV-1 DNA during early ART can lead to drug resistance and virological failure in children.

J Med Virol 2019 Jan 29. Epub 2019 Jan 29.

Division of Infectious Diseases, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore, India.

Using cell-associated DNA and cell-free RNA of human immunodeficiency virus type-1 (HIV-1), we investigated the role of drug-resistant viral variants that emerged during early antiretroviral therapy (ART) in determining virological outcome. This case-control study compared virologic nonresponder children (two viral loads [VLs] ≥ 200 copies/mL within 2 years of ART) and responder children (two VLs < 200 copies/mL after six months of ART) infected with HIV-1 initiated on nonnucleoside reverse-transcriptase inhibitor (NNRTI)-based ART. The partial reverse-transcriptase gene of HIV-1 in cell-associated DNA was genotyped using next-generation sequencing (NGS; Illumina; threshold 0. Read More

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http://doi.wiley.com/10.1002/jmv.25413
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http://dx.doi.org/10.1002/jmv.25413DOI Listing
January 2019
2 Reads

Persistent systemic rotavirus vaccine infection in a child with X-linked severe combined immunodeficiency.

J Med Virol 2019 Jan 27. Epub 2019 Jan 27.

Department of Pediatrics, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Objective: The main aims of the present study were to elucidate the systemic group A rotavirus (RVA) infection and to clarify the genetic changes of persistent virus in the X-linked severe combined immunodeficiency (SCID) patient.

Methods: RotaTeq vaccine (RV5) genotype-specific real-time reverse transcription polymerase chain reaction was used to monitor viral RNA load in serially collected serum and stool samples. Next-generation sequence analysis was used to determine the genotype of the virus by sequencing 11 gene segments. Read More

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http://dx.doi.org/10.1002/jmv.25410DOI Listing
January 2019
2 Reads

Laboratory-based investigation of fever with rash cases in the Maharashtra State - India, 2014 to 2017.

J Med Virol 2019 Jan 24. Epub 2019 Jan 24.

Measles Group, ICMR-National Institute of Virology, Pune, Maharashtra, India.

The Maharashtra State (MS), India, launched measles surveillance in the year 2013. From 2014 to 2017, specimens from the patients presenting with fever and skin rashes were received at the National Institute of Virology, Pune. From 36 districts of the MS, 2795 cases (1428 males and 1367 females) were referred for the laboratory diagnosis of measles and rubella using immunoglobulin M enzyme immunoassay and/or RT-PCRs. Read More

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http://dx.doi.org/10.1002/jmv.25407DOI Listing
January 2019

Variable sources of Bk virus in renal allograft recipients.

J Med Virol 2019 Jan 24. Epub 2019 Jan 24.

Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Sao Paulo, Brasil.

BK virus is the causative agent of polyomavirus-associated nephropathy, a major cause of kidney transplant failure affecting 1%-10% of recipients. Previous studies that investigated the viral source on the kidney recipient pointed that the donor is implicated in the origin of human polyomavirus BK (BKPyV) infection in recipients, but giving the low genetic variability of BKPyV this subject is still controversial. The aim of this study was to determine if BKPyV replicating in kidney recipients after transplantation is always originated from the donor. Read More

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http://dx.doi.org/10.1002/jmv.25409DOI Listing
January 2019

Establishment of sandwich ELISA for detecting the H7 subtype influenza A virus.

J Med Virol 2019 Jan 24. Epub 2019 Jan 24.

National Institute for Viral Disease Control and Prevention, China CDC, Key Laboratory for Medical Virology, National Health Commission, Beijing, P. R. China.

Avian H7N9 subtype influenza virus infects human with high case-fatality rate since it emerged in 2013. Although the vaccination has been rapidly used in poultry due to the emergence of highly pathogenic strain, this virus remains prevalent in this region. Thus, rapid diagnosis both in poultry and human clinic is critically important for the control and prevention of H7N9 infection. Read More

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http://dx.doi.org/10.1002/jmv.25408DOI Listing
January 2019
1 Read

Analytical performance of newly developed rapid point-of-care test for the simultaneous detection of hepatitis A, B, and C viruses in serum samples.

J Med Virol 2019 Jan 22. Epub 2019 Jan 22.

Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

Abstractbackground: Although immunoassays play a major role in the detection of hepatitis viruses, there is a need for a point-of-care (POC) test. We evaluated the EuDx-HE (A,B,C) kit (EUDIPIA, Cheongju, Korea), which detects anti-hepatitis A virus (HAV) immunoglobulin M (IgM), hepatitis B virus surface antigen (HBsAg), and anti-hepatitis C virus (HCV) immunoglobulin G (IgG) simultaneously using an immunochromatographic method within 15 minutes.

Methods: A total of 1581 serum samples including 57, 477, and 451 samples positive for anti-HAV IgM, HBsAg, and anti-HBV IgG, respectively, were analyzed. Read More

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http://doi.wiley.com/10.1002/jmv.25405
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http://dx.doi.org/10.1002/jmv.25405DOI Listing
January 2019
5 Reads

Analysis of genetic and serology of hepatitis A virus infection during and after outbreak in two junior high schools in Surabaya, Indonesia.

J Med Virol 2019 Jan 19. Epub 2019 Jan 19.

Department of Microbiology, School of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.

Outbreaks of hepatitis A have occurred in some cities in Indonesia. In Surabaya, the capital city of East Java province, Indonesia, hepatitis A outbreaks have been reported since2013, with a marked increase in the number of cases in 2015. The aim of the present study was to analyze the genetic and serology of acute symptomatic cases (early infection) during a hepatitis A outbreak and asymptomatic cases after the outbreak in two junior high schools in Surabaya in 2015 to 2016. Read More

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http://dx.doi.org/10.1002/jmv.25403DOI Listing
January 2019
1 Read

Evaluation of a silver-amplified immunochromatography kit for adenoviral conjunctivitis.

J Med Virol 2019 Jan 19. Epub 2019 Jan 19.

Department of Ophthalmology, Fukushima Medical University School of Medicine, Hobara Eye Clinic, Date, Japan.

Objective: To compare and evaluate the sensitivity of a newly developed silver-amplified immunochromatography (SAI) kit with various immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test).

Methods: An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). Read More

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http://doi.wiley.com/10.1002/jmv.25404
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http://dx.doi.org/10.1002/jmv.25404DOI Listing
January 2019
3 Reads

Human metapneumovirus infection: Diagnostic impact of radiologic imaging.

J Med Virol 2019 Jan 19. Epub 2019 Jan 19.

Department of Infectious Diseases and Clinical Microbiology, Koç University, School of Medicine, Istanbul, Turkey.

Background: Human metapneumovirus (hMPV) is a recently detected virus, which can cause mild to severe respiratory tract infections. Through this study, we aimed to detail the outcomes of hMPV infections.

Materials/methods: Between January 2012 and November 2017, patients who had hMPV detected in nasopharyngeal or bronchoalveolar lavage by molecular respiratory pathogen tests were evaluated. Read More

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http://dx.doi.org/10.1002/jmv.25402DOI Listing
January 2019
2 Reads

Functional and clinical consequences of changes to natural killer cell phenotypes driven by chronic cytomegalovirus infections.

J Med Virol 2019 Jan 13. Epub 2019 Jan 13.

Renal Unit, Department of Nephrology, Fiona Stanley Hospital, Murdoch, Western Australia.

Cytomegalovirus (CMV) infections may affect natural killer (NK) cells and are implicated in age-related disorders-notably poor vascular endothelial function. Changes may be greater in renal transplant recipients (RTR) as they have a high burden of CMV and may influence antibody-dependent cellular cytotoxicity (ADCC) responses to viral antigen. We obtained blood mononuclear cells from RTR stable after transplantation (n = 27) and age- and sex-matched controls (n = 28). Read More

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http://doi.wiley.com/10.1002/jmv.25401
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http://dx.doi.org/10.1002/jmv.25401DOI Listing
January 2019
5 Reads

Epidemiology of enteric viruses in children with gastroenteritis in Ogun State, Nigeria.

J Med Virol 2019 Jan 13. Epub 2019 Jan 13.

Virology Laboratory, Infectious Diseases Division, Hospital de Clinicas, Universidade Federal do Parana, Curitiba, Brazil.

Acute gastroenteritis (AGE) remains a global public health concern and Nigeria is one of the two countries accounting for 42% of global under-5 deaths attributable to gastroenteritis. This study aimed to determine the prevalence, seasonality, and risk factors of enteric viruses (EVs) in children with AGE in Ogun State, Nigeria. Stool samples collected from children under-5 from three different hospitals between February 2015 and April 2017 were analyzed using molecular methods for the presence of four EVs (group A rotavirus [RVA], norovirus [NoV], human astrovirus [HAstV], and human adenovirus [HAdV]). Read More

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http://doi.wiley.com/10.1002/jmv.25399
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http://dx.doi.org/10.1002/jmv.25399DOI Listing
January 2019
8 Reads

HEV ORF3 downregulatesCD14 and CD64 to impair macrophages phagocytosis through inhibiting JAK/STAT pathway.

J Med Virol 2019 Jan 13. Epub 2019 Jan 13.

Chongqing Key Laboratory of Infectious Diseases and Parasitic Diseases, Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Hepatitis E virus (HEV) could induce chronic hepatitis and liver failure with high mortality through unknown mechanisms. The previous study showed that the HEV open reading frames 3 (ORF3) could inhibit macrophages inflammatory response. Impaired macrophages phagocytosis was also found in patients infected with HEV and its nucleic acids could be detected in macrophages. Read More

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http://doi.wiley.com/10.1002/jmv.25400
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http://dx.doi.org/10.1002/jmv.25400DOI Listing
January 2019
7 Reads

Genetic variants in IFIH1 and DDX58 influence hepatitis C virus clearance in Chinese Han population.

J Med Virol 2019 Jan 11. Epub 2019 Jan 11.

Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, China.

Aims: To investigate the association between two RIG-I-like receptor gene polymorphisms and hepatitis C virus (HCV) infection in Chinese Han population.

Methods: The current study genotyped two selected SNPs (IFIH1 rs3747517 and DDX58 rs9695310) using TaqMan allelic discrimination assay to assess their association with the susceptibility and clinical outcome of HCV infection among 3065 participants (1545 non-HCV infection individuals, 568 spontaneous HCV clearance cases, and 952 persistent infection patients).

Results: IFIH1 rs3747517 (dominant model: Adjusted odds ratio [OR] = 1. Read More

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http://doi.wiley.com/10.1002/jmv.25398
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http://dx.doi.org/10.1002/jmv.25398DOI Listing
January 2019
3 Reads

Impact of HPyV9 and TSPyV coinfection on the development of BK polyomavirus viremia and associated nephropathy after kidney transplantation.

J Med Virol 2019 Jan 9. Epub 2019 Jan 9.

Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.

Background: BK polyomavirus (BKPyV) persistently infects the urinary tract and causes viremia and nephropathy in kidney transplantation (KTx), recipients. In a previous study, we observed an increased incidence and load of BKPyV viremia in KTx patients coinfected with human polyomavirus 9 (HPyV9). Here we sought confirmation of this observation and explored whether novel HPyVs that have been detected in urine (HPyV9 and trichodysplasia spinulosa polyomavirus [TSPyV]) potentially aggravate BKPyV infection. Read More

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http://doi.wiley.com/10.1002/jmv.25397
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January 2019
2 Reads

Association between miRNA-146a rs2910164 (G/C) polymorphism with the susceptibility to chronic HBV infection and spontaneous viral clearance in an Iranian population.

J Med Virol 2019 Jan 9. Epub 2019 Jan 9.

Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Hepatitis B virus (HBV) infection is one of the clinical dilemmas in chronic liver diseases. MicroRNAs (miRNAs) are small noncoding RNA molecules that play an important role in the pathogenesis of liver diseases and single nucleotide polymorphisms (SNPs) in miRNA genes affect the clinical course of HBV infection. Previous studies have shown that miRNA-146a rs2910164 polymorphism can be associated with the pathogenesis of liver diseases such as hepatocellular carcinoma. Read More

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http://doi.wiley.com/10.1002/jmv.25394
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http://dx.doi.org/10.1002/jmv.25394DOI Listing
January 2019
2 Reads
2.347 Impact Factor

Memantine can relieve the neuronal impairment caused by neurotropic virus infection.

J Med Virol 2019 Jan 9. Epub 2019 Jan 9.

Department of Animal Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Neurotropic viruses, such as the rabies virus (RABV) and Japanese encephalitis virus (JEV), induce neuronal dysfunction and complication, causing neuronal damage. Currently, there are still no effective clinical treatments for neuronal injury caused by neurotropic viruses. Memantine, a drug capable of passing through the blood-brain barrier, noncompetitively and reversibly binds to n-methyl- d-aspartic acid (NMDA) receptors. Read More

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http://doi.wiley.com/10.1002/jmv.25396
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January 2019
11 Reads
2.347 Impact Factor

Epidemiological and molecular investigation of norovirus and astrovirus infections in Rio Branco, Acre, Northern Brazil: A retrospective study.

J Med Virol 2019 Jan 9. Epub 2019 Jan 9.

Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health, Ananindeua, Pará, Brazil.

Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis (AGE) outbreaks worldwide, with infections reported in semiclosed environments, particularly in hospitals and nursing homes. Astrovirus (HAstV) is prevalent worldwide, especially in developing countries. We aimed to determine the prevalence, spatial distribution, and genetic diversity of NoV and HAstV in children under 5 years of age in Rio Branco city, Acre State, Amazon Region, Brazil. Read More

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http://dx.doi.org/10.1002/jmv.25395DOI Listing
January 2019

High prevalence of enteric viruses associated with acute gastroenteritis in pediatric patients in a low-income area in Vitória, Southeastern Brazil.

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

Laboratório de Saneamento, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil.

Acute gastroenteritis (AGE) is a significant cause of child mortality worldwide. In Brazil, despite the reduction in infant mortality achieved in recent years, many children still die because of undiagnosed AGE. The prevalence, viral load, and circulating genotypes of rotavirus A (RVA), human adenovirus (HAdV), and norovirus GII (NoV GII) were investigated in children with AGE during 12 months in Vitoria, Espírito Santo, Southeastern Brazil. Read More

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http://doi.wiley.com/10.1002/jmv.25392
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January 2019
12 Reads

Ongoing change of severe hand, foot, and mouth disease pathogens in Yunnan, China, 2012 to 2016.

J Med Virol 2019 Jan 6. Epub 2019 Jan 6.

The Department of Medical Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, China.

Hand, foot, and mouth disease (HFMD) is a common infectious disease caused by enteroviruses (EVs). In this study, a total of 341 children with serious HFMD were admitted to a pediatric hospital in Yunnan, China in 2012 to 2016. EVs were detected in 283 specimens (83. Read More

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http://doi.wiley.com/10.1002/jmv.25393
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January 2019
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Evaluation of Varicella-zoster virus-specific cell-mediated immunity by interferon-γ Enzyme-Linked Immunosorbent Assay in adults ≥50 years of age administered a herpes zoster vaccine.

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, China.

Varicella-zoster virus (VZV)-specific cell-mediated immunity (CMI) is critical for preventing and controlling the onset of herpes zoster (HZ). To assess VZV CMI, an interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) was validated by examining the influence of VZV-specific antigen content, incubation time, and interval from whole blood collection on the assay. In phase II clinical trial, VZV-specific CMI in adults ≥50 years of age administered an HZ vaccine were evaluated by IFN-γ ELISA, as determined by measuring IFN-γ production in the whole blood in response to stimulation with ultraviolet light-inactivated VZV. Read More

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http://dx.doi.org/10.1002/jmv.25391DOI Listing
January 2019
3 Reads
2.347 Impact Factor

Genetic analyses of Seoul hantavirus genome recovered from rats (Rattus norvegicus) in the Netherlands unveils diverse routes of spread into Europe.

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

Department of Virology, Medicum, University of Helsinki, Helsinki, Finland.

Seoul virus (SEOV) is the etiologic agent of hemorrhagic fever with renal syndrome. It is carried by brown rats (Rattus norvegicus), a commensal rodent that closely cohabitates with humans in urban environments. SEOV has a worldwide distribution, and in Europe, it has been found in rats in UK, France, Sweden, and Belgium, and human cases of SEOV infection have been reported in Germany, UK, France, and Belgium. Read More

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http://doi.wiley.com/10.1002/jmv.25390
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http://dx.doi.org/10.1002/jmv.25390DOI Listing
January 2019
7 Reads

Prevalence of antibodies against BKPyV subtype I and IV in kidney transplant recipients and in the general Czech population.

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

Department of Genetics and Microbiology, Faculty of Science, Charles University, Prague, Czech Republic.

Active infection with BK polyomavirus (BKPyV) may cause serious complications in transplantation settings. Recently, the level of BKPyV IgG seroreactivity in graft donors has been shown to predict viremia and BKPyV-associated nephropathy in kidney transplant (KTx) recipients. Pretransplantation testing of the donor and recipient BKPyV serostatus could, therefore, identify patients at high risk. Read More

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http://doi.wiley.com/10.1002/jmv.25388
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January 2019
6 Reads

Cytomegalovirus immunoglobulin G titers do not predict reactivation risk in immunocompetent hosts.

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

Department of Surgery, Beth Israel Deaconess Medical Center/Harvard Medical School, Boston, Massachusetts.

Cytomegalovirus (CMV) reactivation occurs in roughly one-third of immunocompetent patients during critical illness, and is associated with worse outcomes. These outcomes have prompted consideration of early antiviral prophylaxis, but two-third of patients would receive unnecessary treatment. Tissue viral load has been associated with risk of reactivation in murine models, and recent work has suggested a relationship between immune responses to CMV and underlying viral load. Read More

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http://doi.wiley.com/10.1002/jmv.25389
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January 2019
9 Reads

Epstein-Barr virus LMP2A utilizes Syk and PI3K to activate NF-κB in B-cell lymphomas to increase MIP-1α production.

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

Department of Microbiology and Immunology, College of Graduate Studies, Midwestern University, Downers Grove, Illinois.

The incidence of Hodgkin's lymphoma (HL) is growing due to an increase in Epstein-Barr virus (EBV)-associated HL in AIDS patients. The HL tumor microenvironment is vital for the survival of the malignant Hodgkin-Reed Sternberg (HRS) cells of HL, which express the EBV protein latent membrane protein 2A (LMP2A). While previous work shows that LMP2A mimics B-cell receptor (BCR) signaling to promote the survival of HRS cells, the ability of LMP2A to establish and maintain the tumor microenvironment through the production of chemokines remains unknown. Read More

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http://dx.doi.org/10.1002/jmv.25381DOI Listing
January 2019

A sandwich ELISA for detecting the hemagglutinin of avian influenza A (H10N8) virus.

J Med Virol 2018 Dec 29. Epub 2018 Dec 29.

National Institute for Viral Disease Control and Prevention, China CDC, Key Laboratory for Medical Virology, National Health Commission of P. R. China, Beijing, China.

Novel influenza A virus (H10N8) infected human with fatality in China during 2013-2014. It is important to detect such nonprevalent subtype influenza A virus in clinic and regular surveillance in the early stage for effective control and prevention from the potential pandemic. Unavailability of convenient rapid diagnosis for this subtype virus in resources-limited setting is an obstacle for timely recognizing human case. Read More

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http://dx.doi.org/10.1002/jmv.25387DOI Listing
December 2018
2 Reads

Cytomegalovirus shedding from breastmilk and mucosal sites in healthy postpartum women: A pilot study.

J Med Virol 2018 Dec 22. Epub 2018 Dec 22.

Departments of Medicine and Laboratory Medicine, University of Washington, Seattle, WA.

Mother-to-child cytomegalovirus (CMV) breastmilk transmission can occur in the postnatal period. In a pilot study, we measured daily CMV detection by polymerase chain reaction in breastmilk, vaginal, and saliva samples from nine healthy CMV-seropositive postpartum women for 28 days. CMV was found in seven of nine women and 171 of 253 breastmilk samples (67. Read More

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http://dx.doi.org/10.1002/jmv.25386DOI Listing
December 2018

Influence of HLA-B18 on liver fibrosis progression in a cohort of HIV/HCV coinfected individuals.

J Med Virol 2018 Dec 22. Epub 2018 Dec 22.

Department of Biomedical Sciences and Human Oncology, Clinic of Infectious Diseases, University of Bari "Aldo Moro,", Bari, Italy.

Liver fibrosis is accelerated in human immunodeficiency virus/hepatitis C virus (HIV/HCV) coinfected compared with HCV monoinfected patients, due to multiple cofactors. Recently, HLA-B18 haplotype has been associated with short-term liver disease progression in this population. Our aim was to assess the influence of HLA-B18 on the fibrosis process in HIV/HCV coinfected individuals, untreated for HCV, during a long-term follow-up. Read More

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http://dx.doi.org/10.1002/jmv.25385DOI Listing
December 2018

Evaluation of the antiviral potential of the soluble forms of glycoprotein D receptors on ocular herpes caused by HSV-1 and HSV-2 infections in a transgenic mouse model.

J Med Virol 2018 Dec 21. Epub 2018 Dec 21.

Department of Biomedicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Ocular herpes, caused by herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infections, remains an important corneal disease, which may result in loss of vision. Because the frequency of acyclovir resistance in HSV has increased, novel antiviral agents are needed for therapeutic approaches to ocular herpes. Several studies have demonstrated that fusion proteins containing entire ectodomain of HSV glycoprotein D receptors, including herpesvirus entry mediator A (HVEM), nectin-1 and nectin-2, and the Fc portion of human IgG (HVEMIg, nectin-1Ig, and nectin-2Ig, respectively), can exert antiviral effects in vitro and in vivo. Read More

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http://dx.doi.org/10.1002/jmv.25384DOI Listing
December 2018
2.347 Impact Factor