8,467 results match your criteria Journal of Medical Virology[Journal]


Chandipura Virus changes cellular miRNome in human microglial cells.

J Med Virol 2019 Apr 24. Epub 2019 Apr 24.

Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi.

Chandipura virus (CHPV) is a neurotropic virus, known to cause encephalitis in humans. The microRNAs play an important role in the pathogenesis of viral infection. The present study is focused on the role of miRNAs during CHPV (Strain 1653514) infection in human microglial cells. Read More

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http://dx.doi.org/10.1002/jmv.25491DOI Listing

Hepatitis B core antigen can regulate NLRP3 inflammasome pathway in HepG2 cells.

J Med Virol 2019 Apr 24. Epub 2019 Apr 24.

Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Hepatitis B virus(HBV) has four open reading frames (ORFs) of which ORF C is consists of the pre Core and Core genes encodes the Hepatitis B core antigen (HBcAg) and Hepatitis B e antigen (HBeAg). Studies have shown that HBeAg significantly inhibits the NLRP3 inflammasome activation and IL-1β production. However, the role of HBcAg and ORF C proteins (in this paper, ORF C proteins = HBcAg + HBeAg) were remain unclear. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25490
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http://dx.doi.org/10.1002/jmv.25490DOI Listing

Viruses associated with acute respiratory infection in a community-based cohort of healthy NZ children.

J Med Virol 2019 Apr 24. Epub 2019 Apr 24.

Virology Research Laboratory, Prince of Wales Hospital, Sydney, NSW, Australia.

Acute respiratory infections (ARIs) are a major cause of morbidity among children. Respiratory viruses are commonly detected in both symptomatic and asymptomatic periods. The rates of infection, and community epidemiology of respiratory viruses in healthy children needs further definition to assist interpretation of molecular diagnostic assays in this population. Read More

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http://dx.doi.org/10.1002/jmv.25493DOI Listing

Seroepidemiology of respiratory syncytial virus in western India with special reference to appropriate age for infant vaccination.

J Med Virol 2019 Apr 23. Epub 2019 Apr 23.

Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune, India.

Respiratory Syncytial Virus (RSV) causes significant infant mortality worldwide and a vaccine may be available soon. This study determined age-stratified anti-RSV antibody positivity (ELISA) at Pune, India (cord blood-85years). Antibody positivity declined from 100% at birth to 71. Read More

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http://dx.doi.org/10.1002/jmv.25489DOI Listing

Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune competence.

J Med Virol 2019 Apr 19. Epub 2019 Apr 19.

Department of Translational Research, University of Pisa, Pisa, Italy.

Background: Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict post-transplant immune-related complications, and eventually to customize immunosuppression.

Methods: In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE® assay. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25488
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http://dx.doi.org/10.1002/jmv.25488DOI Listing
April 2019
1 Read

Herpesviral infections and antimicrobial protection for Alzheimer's disease: Implications for prevention and treatment.

Authors:
Qingsong Qin Yun Li

J Med Virol 2019 Apr 17. Epub 2019 Apr 17.

Sleep Medicine Center, Shantou University Medical College, Shantou, Guangdong, China.

Accumulating evidence suggests that infections by herpesviruses might be closely linked to Alzheimer's disease (AD). Pathological hallmarks of AD brains include senile plaques induced by amyloid β peptide (Aβ) in the extracellular space and intracellular neurofibrillary tangles (NFTs) consisting of phosphorylated tau protein. The prevailing hypothesis for the mechanism of AD is amyloid cascade reaction. Read More

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http://dx.doi.org/10.1002/jmv.25481DOI Listing
April 2019
1 Read

Curaxin CBL0137 has the potential to reverse HIV-1 latency.

J Med Virol 2019 Apr 16. Epub 2019 Apr 16.

Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.

A cure for human immunodeficiency virus type-1 (HIV-1) has been hampered by the limitation of current combination antiretroviral therapy (cART) to address the latent reservoirs in HIV-1 patients. One strategy proposed to eradicate these reservoirs is the "shock and kill" approach, where latency-reversing agents (LRAs) are used to reactivate and promote viral cell death and/or immune killing of reactivated cells. Here, we report that curaxin CBL0137, an anti-tumor compound, can potentiate TNFα-mediated reactivation of latently infected HIV-1cell lines. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25487
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http://dx.doi.org/10.1002/jmv.25487DOI Listing
April 2019
3 Reads

Human bocavirus detection and quantification in fecal and serum specimens from recipients of allogeneic hematopoietic stem cell transplantation: a longitudinal study.

J Med Virol 2019 Apr 14. Epub 2019 Apr 14.

Laboratory of Virology and Cell Culture, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brazil.

Objectives: The aim of this study was to evaluate the occurrence of human bocavirus (HBoV) and to determine viral loads in samples of patients admitted for allogeneic hematopoietic stem cell transplantation (allo-HSCT).

Methods: Fecal and serum samples were collected from 19 patients, during a 24-month period. Samples were screened by qPCR TaqMan assay, with specific probe and primers targeting the NP1 gene of all HBoVs genotypes (HBoV-1 to -4), and viral loads were determined using serial dilutions of a recombinant plasmid. Read More

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http://dx.doi.org/10.1002/jmv.25486DOI Listing
April 2019
1 Read

Measurement of melatonin, indole-dioxygenase, IL-6, IL-18, ferritin, CRP, and total homocysteine levels during herpes zoster.

J Med Virol 2019 Apr 12. Epub 2019 Apr 12.

Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

The risk of herpes zoster (HZ) increases with age and declining immune function. Increased oxidative stress and inflammatory conditions may cause a negative impact on the immune responses. The present study aimed to assess the levels of oxidative/inflammatory stress biomarkers in HZ patients compared with the controls. Read More

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http://dx.doi.org/10.1002/jmv.25484DOI Listing
April 2019
2.347 Impact Factor

Evaluation of dried blood spots for hepatitis B and D serology and nucleic acid testing.

J Med Virol 2019 Apr 12. Epub 2019 Apr 12.

Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital and WHO Regional Reference Laboratory for Hepatitis B/D, at The Peter Doherty Institute for Infection and Immunity, Victoria, Australia.

Areas with the highest burden of hepatitis B virus (HBV) infection are often low-middle-income countries with limited access to diagnosis due to isolation, affordability and/or feasibility. Dried blood spots (DBS) provide an alternative for remote areas where collection and transportation of serum is impractical. In this study the application of DBS for serological and molecular detection of HBV and hepatitis D virus (HDV) was evaluated. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25485
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http://dx.doi.org/10.1002/jmv.25485DOI Listing
April 2019
6 Reads

MicroRNA-34a Aggravates Coxsackievirus B3-Induced Apoptosis of Cardiomyocytes through the SIRT1-p53 Pathway.

J Med Virol 2019 Apr 10. Epub 2019 Apr 10.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Shanghai Medical College of Fudan University, Shanghai, China.

Viral myocarditis is inflammation of the myocardium mainly caused by a viral infection, and coxsackievirus B3 (CVB3) infection is one of the most common. It is well known that cardiomyocyte apoptosis is involved in the pathogenesis of viral myocarditis. MicroRNAs (miRNAs, miRs) are endogenous noncoding oligoribonucleotides involved in various pathological conditions, and miR-34a is one of the miRNAs causing apoptosis. Read More

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http://dx.doi.org/10.1002/jmv.25482DOI Listing
April 2019
1 Read

Long noncoding RNA TSPOAP1 antisense RNA 1 negatively modulates type I IFN signaling to facilitate influenza A virus replication.

J Med Virol 2019 Apr 10. Epub 2019 Apr 10.

Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.

Although the expression of thousands of host long noncoding RNAs (lncRNAs) can be regulated by viral infection, the number of lncRNAs with experimentally verified function is limited. In this study, the expression of host lncRNA TSPOAP1-AS1 was significantly induced by influenza A virus (IAV) infection in a dose- and time-dependent manner. Polyinosine-polycytidylic acid (poly (I:C)), a synthetic analog of double-stranded RNA, also increased TSPOAP1-AS1 expression. Read More

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http://dx.doi.org/10.1002/jmv.25483DOI Listing

High success rates for the use of ombitasvir/paritaprevir/ritonavir containing regimens in treatment of naïve and experienced chronic hepatitis C genotype 4: Real world results.

J Med Virol 2019 Apr 5. Epub 2019 Apr 5.

Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Cairo, Egypt.

Introduction And Aims: Treatment of hepatitis C virus (HCV) genotype 4 patient with fixed dose combination of ombitasvir-paritaprevir-ritonavir plus ribavirin (OBV/rPTV/RBV) has been proven efficacy and safety in many clinical trials. The current study reports the efficacy and safety of OBV/rPTV/RBV (for treatment-naïve), and OBV/rPTV/RBV/sofosbuvir (SOF) (for treatment-experienced), in chronic HCV genotype 4 patients in real life settings.

Methods: Prospective cohort study including all adult chronic HCV genotype 4 patients who were scheduled to receive OBV/rPTV/RBV ± SOF for 12 or 24 weeks in New Cairo Viral Hepatitis Treatment Center. Read More

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http://dx.doi.org/10.1002/jmv.25478DOI Listing
April 2019
1 Read

Prevalence and genotype distribution of human papillomavirus infection among women: A population-based study in Dali Bai Autonomous Prefecture, Yunnan Province, China.

J Med Virol 2019 Apr 5. Epub 2019 Apr 5.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Dali University, Dali, China.

Accumulating evidence indicates that persistent infection with high-risk oncogenic human papillomavirus (HPV) genotypes is the most important risk factor for cervical cancer, and that the distribution of HPV genotypes varies regionally. This study explored the prevalence and genotype distribution of HPV infection among Han, Yi, and Bai women in various regions of Dali Bai Autonomous Prefecture, Yunnan Province, China. This cross-sectional study included 2779 women (20-76 years old) who were referred for 21-HPV genotype array diagnostic from five regions of Dali Bai Autonomous Prefecture between February 2013 and May 2016. Read More

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http://dx.doi.org/10.1002/jmv.25477DOI Listing
April 2019
3 Reads
2.347 Impact Factor

Impact of molecular point-of-care testing on clinical management and in-hospital costs of patients suspected of influenza or RSV infection: a modeling study.

J Med Virol 2019 Apr 5. Epub 2019 Apr 5.

Department of Health Evidence, Radboud University Medical Center, Nijmegen, The Netherlands.

Background: At hospital admission, patients suspected of infection with influenza or respiratory syncytial virus (RSV) are placed in isolation, pending the outcome of diagnostics. In a significant number, isolated care proves unnecessary. We investigated the potential impact of molecular point-of-care (POC) diagnostics on patient management and in-hospital costs. Read More

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http://dx.doi.org/10.1002/jmv.25479DOI Listing
April 2019
1 Read

Genetic variations in death receptor domain 4 (DR4) gene and the susceptibility to hepatitis C related hepatocellular carcinoma.

J Med Virol 2019 Apr 4. Epub 2019 Apr 4.

Associate lecturer of Clinical Pathology HematologyDepartment, Theodor Bilharz Research Institute, Cairo, Egypt.

Background: Hepatocellular Carcinoma (HCC) is a leading cause of cancer mortality worldwide particularly in Egypt. The role of apoptosis in tumorigenesis has been well-documented and resistance to apoptosis is a hallmark of cancer. Several studies discussed the association between death receptor 4 (DR4) genetic variants and HCC risk. Read More

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http://dx.doi.org/10.1002/jmv.25476DOI Listing
April 2019
1 Read

Distinct expression and clinical value of aquaporin 4 in children with hand, foot and mouth disease caused by enterovirus 71.

J Med Virol 2019 Apr 3. Epub 2019 Apr 3.

Department of Intensive Care Unit, Xuzhou Children's Hospital of Xuzhou Medical University, Xuzhou, China.

Brain dysfunction is a prerequisite for critical complications in children with hand, foot, and mouth disease (HFMD). Aquaporin 4 (AQP-4) may be involved in the pathological process of cerebral oedema and injury in children with severe and critical HFMD. This study aimed to assess the association of AQP-4 with the severity of enterovirus 71 (EV71)-associated HFMD. Read More

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http://dx.doi.org/10.1002/jmv.25475DOI Listing
April 2019
3 Reads

Viruses with masked pathogenicity and genetically susceptible hosts-How to discover potentially pathogenic viruses.

J Med Virol 2019 Mar 30. Epub 2019 Mar 30.

Division of Host Defense, Research Center for the Prevention of Infectious Diseases, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

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http://dx.doi.org/10.1002/jmv.25472DOI Listing
March 2019
1 Read

Differential age-specific distribution of influenza virus types and subtypes in tropical Singapore, 2011 to 2017.

J Med Virol 2019 Mar 30. Epub 2019 Mar 30.

National Public Health Laboratory, National Centre for Infectious Diseases, Singapore.

Surveillance and reporting of epidemiological features of seasonal influenza mostly are aggregates across all-ages. We investigated age-specific differences in distribution of influenza virus (sub)types in tropical Singapore, using laboratory-confirmed virological data collected under the national influenza surveillance programme from 2011 to 2017. The proportion of influenza-positive specimens from outpatients with influenza-like illness was used as an indicator of influenza activity in the community. Read More

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http://dx.doi.org/10.1002/jmv.25473DOI Listing

Routine drug resistance testing in proviral HIV-1 DNA: Prevalence of stop codons and hypermutation, and associated factors.

J Med Virol 2019 Mar 30. Epub 2019 Mar 30.

Faculté de Médecine, CHU Lille, Laboratoire de Virologie EA3610, Univ Lille, Lille, France.

We investigated the presence of stop codons (SC) and/or hypermutation (HM) in HIV-1 DNA sequences generated for routine drug resistance testing in proviral HIV-1 DNA, and sought for associated factors. At least one SC was identified in 6.2% of HIV-1 DNA sequences, among which 54. Read More

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http://dx.doi.org/10.1002/jmv.25474DOI Listing
March 2019
1 Read

Computational identification of hepatitis E virus-encoded microRNAs and their targets in human.

J Med Virol 2019 Mar 28. Epub 2019 Mar 28.

Department of Biotechnology, Gauhati University, Guwahati, Assam, India.

microRNAs (miRNAs) are small, noncoding RNAs which regulate eukaryotic gene expression via RNA interference pathway. Recently, miRNAs have been identified in a number of viruses with current evidence suggesting that they regulate gene expression in both virus and host. This makes viral miRNAs potential targets of clinical intervention, with the possibility of inhibiting aberrant host gene expression associated with the disease. Read More

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http://dx.doi.org/10.1002/jmv.25471DOI Listing
March 2019
2 Reads

Influenza surveillance during the 2009-2010, 2010-2011, 2011-2012, and 2012-2013 seasons in Algeria.

J Med Virol 2019 Mar 25. Epub 2019 Mar 25.

Department of Virology, Psteur Institute of Algeria, Sidi-Fredj, Algiers, Algeria.

We report the activity and circulation of influenza viruses in Algeria during four influenza seasons, from a national surveillance study carried out from 2009-2010 to 2012-2013. A total of 2766 samples from in- and outpatients, with no age restriction, were collected. The overall proportion of specimens that tested influenza positive was 46. Read More

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http://dx.doi.org/10.1002/jmv.25469DOI Listing
March 2019
2 Reads

Molecular and serological characterization of occult hepatitis B virus infection among patients with hemophilia.

J Med Virol 2019 Mar 25. Epub 2019 Mar 25.

Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.

Background: The occult hepatitis B virus infection (OBI) is a health concern among high-risk groups and immunosuppressed individuals. There is still a paucity of data regarding the occult hepatitis B virus infection among hemophilic patients. With this in mind, we aimed to evaluate the molecular prevalence of OBI among clients with hemophilia. Read More

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http://dx.doi.org/10.1002/jmv.25470DOI Listing
March 2019
1 Read

Myeloid-derived suppressor cells expansion is closely associated with disease severity and progression in HBV-related acute-on-chronic liver failure.

J Med Virol 2019 Mar 24. Epub 2019 Mar 24.

Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Dysregulation of the host immune responses induced by host hepatitis B virus (HBV) interactions has been observed in acute-on-chronic liver failure (ACLF). Myeloid-derived suppressor cells (MDSCs), well known for their immunomodulatory properties, can suppress T-cell function by regulating the expression of CD3 ζ chain in cancer and autoimmune/infectious diseases while rarely have been studied in ACLF. In this study, MDSCs, CD4 /CD8 T cells, and CD3 ζ chain were analyzed by flow cytometry in peripheral blood mononuclear cells obtained from HBV-related ACLF patients, chronic hepatitis B (CHB) patients and healthy controls. Read More

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http://dx.doi.org/10.1002/jmv.25466DOI Listing
March 2019
1 Read

Absolute lymphocyte count and human adenovirus-specific T-cell immune restoration of human adenovirus infection after kidney transplantation.

J Med Virol 2019 Mar 24. Epub 2019 Mar 24.

Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

Introduction: Human adenovirus (HAdV) infection can cause substantial morbidity in kidney transplant (KT) recipients. Cell-mediated immunity plays an important role in controlling HAdV infection after KT.

Methods: We prospectively (January 2015 to June 2018) investigated the absolute lymphocyte count (ALC) and interferon-γ-producing CD4 and CD8 T cells at diagnosis and at viral clearance by an intracellular cytokine assay after stimulating with HAdV whole lysate, hexon, and penton proteins. Read More

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http://dx.doi.org/10.1002/jmv.25468DOI Listing

Apoptosis and its relation with clinical course in patients with Crimean-Congo hemorrhagic fever.

J Med Virol 2019 Mar 24. Epub 2019 Mar 24.

Department of Infectious Diseases and Clinical Microbiology, Cumhuriyet University School of Medicine, Sivas, Turkey.

Crimean-Congo hemorrhagic fever (CCHF) is a tick-mediated viral infection. Patients with CCHF may show various clinical presentations. The cause of this difference in the clinical course is not completely understood. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25467
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http://dx.doi.org/10.1002/jmv.25467DOI Listing
March 2019
2 Reads

On-treatment changes of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein are associated with the regression of liver fibrosis in chronic hepatitis B patients on interferon α add-on therapy.

J Med Virol 2019 Mar 24. Epub 2019 Mar 24.

Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis & National Clinical Research Center of Digestive Diseases, Beijing, China.

Wisteria floribunda agglutinin-positive Mac-2-binding protein (M2BP) has been identified as a predictor for the response of interferon α (IFN-α) in patients with viral hepatitis. However, whether serum glycosylation isomer of M2BP (M2BPGi) was associated with the regression of liver fibrosis in patients with chronic hepatitis B (CHB) during IFN-α add-on therapy is still unknown. CHB patients were treated with entecavir for 26 weeks followed by entecavir plus pegylated IFN-α for 52 weeks. Read More

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http://dx.doi.org/10.1002/jmv.25465DOI Listing
March 2019
2 Reads

An antiviral drug screening system for enterovirus 71 based on an improved plaque assay: A potential high-throughput method.

J Med Virol 2019 Mar 22. Epub 2019 Mar 22.

Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Plaque assay plays an irreplaceable role in a variety of virological studies, including determining titers of viruses. Our previous study showed that a simple and highly repeatable plaque assay could be used for enterovirus 71 (EV-A71). Now, we show that using a subclone of a clinical EV-A71 isolate and a rhabdomyosarcoma cell line (RD), a plaque assay based on an EV-A71/RD model could exhibit the most rapid formation of plaques (<2 days), with much higher repeatability and consistency. Read More

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http://dx.doi.org/10.1002/jmv.25463DOI Listing
March 2019
9 Reads
2.347 Impact Factor

Incidence and seasonality of respiratory viruses causing acute respiratory infections in the Northern United Arab Emirates.

J Med Virol 2019 Mar 22. Epub 2019 Mar 22.

Department of Laboratory Medicine, Sheikh Khalifa Specialty Hospital, Ras Al Khaimah, UAE.

Background: The data on the seasonality of respiratory viruses helps to ensure the optimal vaccination period and to monitor the possible outbreaks of variant type.

Objectives: This study was designed to describe the molecular epidemiology and seasonality of acute respiratory infection (ARI)-related respiratory viruses in the United Arab Emirates (UAE).

Methods: Both upper and lower respiratory specimens were collected for the analysis from all the patients who visited the Sheikh Khalifa Specialty Hospital (SKSH) with ARI for over 2 years. Read More

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http://dx.doi.org/10.1002/jmv.25464DOI Listing
March 2019
3 Reads

Respiratory syncytial virus infection among adults during influenza season: A frequently overlooked diagnosis.

J Med Virol 2019 Mar 22. Epub 2019 Mar 22.

Department of Internal Medicine, Hospital Universitario de Guadalajara, Guadalajara, Spain.

Our objective is to assess the characteristics of respiratory syncytial virus (RSV) infection in adult patients and to establish differences with influenza viruses. Fifty-four patients diagnosed with RSV and 198 with influenza were prospectively included. Compared with influenza, empirical antimicrobial therapy was more frequent in patients diagnosed with RSV, whereas antibiotic withdrawal at the time of diagnosis confirmation was lower (OR, 0. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25462
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http://dx.doi.org/10.1002/jmv.25462DOI Listing
March 2019
4 Reads

Asymptomatic hepatitis B carriers who were vaccinated at birth.

J Med Virol 2019 Mar 18. Epub 2019 Mar 18.

Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Guangxi Key Laboratory for the Prevention and Control of Viral Hepatitis, Nanning, Guangxi, China.

The long-term persistence of immunity following universal infant immunization against hepatitis B virus (HBV) and the need for a subsequent booster dose in adolescence remain under debate. With data derived from Long'an County, Guangxi, China, we reported previously that the prevalence of hepatitis B surface antigen (HBsAg) among adults born from 1987 to 1993 increases with age, although these individuals had received a first dose of the vaccine within 24 hours of birth. Here, we sought the source of transmission by comparison of genotypes among their family members using phylogenetic analysis of complete HBV S gene sequences. Read More

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http://dx.doi.org/10.1002/jmv.25461DOI Listing
March 2019
1 Read

Immune and cytokine/chemokine responses of PBMCs in rotavirus-infected rhesus infants and their significance in viral pathogenesis.

J Med Virol 2019 Mar 15. Epub 2019 Mar 15.

Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming, China.

The rotavirus (RV) is the most important causative agent of severe gastroenteritis in infants and children aged less than 5 years worldwide. However, the response and the roles of peripheral blood mononuclear cell (PBMC) in RV clearance have yet to be fully elucidated. In this study, we established the neonatal rhesus monkey model of RV infection with histopathological changes in the small intestine. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25460
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http://dx.doi.org/10.1002/jmv.25460DOI Listing
March 2019
14 Reads

Prevalence and risk factors of hepatitis B virus infection in Middle-Limburg Belgium, year 2017: Importance of migration.

J Med Virol 2019 Mar 14. Epub 2019 Mar 14.

Department of Gastro-Enterology and Hepatology, Ziekenhuis Oost-Limburg, Genk, Belgium.

Background And Aim: The hepatitis B virus (HBV) prevalence study performed in 2003 in Belgium is believed to be underestimating HBV prevalence due to underrepresentation of the non-Belgian population. Therefore, we assessed the prevalence and risk factors of HBV infection in a multi-ethnic region situated in Middle-Limburg Belgium, in 2017.

Methods: Between May and November 2017, blood samples and questionnaires were taken from patients who presented at the emergency department of a large educational hospital. Read More

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http://dx.doi.org/10.1002/jmv.25457DOI Listing
March 2019
4 Reads

A 28-year study of human parainfluenza in Rio Grande do Sul, Southern Brazil.

J Med Virol 2019 Mar 13. Epub 2019 Mar 13.

Departamento de Ciências Básicas da Saúde, Laboratório de Biologia Molecular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, Rio Grande do Sul, Brazil.

Problem: Human parainfluenza virus (hPIV) is an important pathogen in respiratory infections, however the health burden of hPIV is underestimated. This study describes the infections by hPIV1-3 in Rio Grande do Sul, Brazil, from 1990 to 2017, providing data of the frequency and seasonality of cases and associated clinical symptoms.

Method Of Study: Nasopharyngeal samples of patients with respiratory infection were collected, clinical data were analyzed, and immunofluorescence was used to detect hPIV. Read More

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http://doi.wiley.com/10.1002/jmv.25459
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http://dx.doi.org/10.1002/jmv.25459DOI Listing
March 2019
10 Reads

Rhinoviruses in infancy and risk of immunoglobulin E sensitization.

J Med Virol 2019 Mar 13. Epub 2019 Mar 13.

Department of Virology, Faculty of Medical Sciences and Biotechnology, Tampere University, Tampere, Finland.

Previous data about the role of viruses in the development of allergic immunoglobulin E (IgE) sensitization are contradictory. The aim of this study was to determine the possible associations between exposure to different viruses (rhinovirus, enterovirus, norovirus, and parechovirus) during the first year of life and IgE sensitization. Viruses were analyzed from stool samples collected monthly from infants participating in a prospective birth cohort study. Read More

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http://doi.wiley.com/10.1002/jmv.25455
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http://dx.doi.org/10.1002/jmv.25455DOI Listing
March 2019
7 Reads

Genetic diversity of influenza A(H3N2) viruses in Northern Cameroon during the 2014-2016 influenza seasons.

J Med Virol 2019 Mar 13. Epub 2019 Mar 13.

National Influenza Centre, Centre Pasteur of Cameroon, Yaoundé, Cameroon, PO Box 1274, Yaoundé, Cameroon.

In Cameroon, genome characterization of influenza virus has been performed only in the Southern regions meanwhile genetic diversity of this virus varies with respect to locality. The Northern region characterized by a Sudan tropical climate might have distinct genetic characterization. This study aimed to better understand the genetic diversity of influenza A(H3N2) viruses circulating in Northern Cameroon. Read More

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http://dx.doi.org/10.1002/jmv.25456DOI Listing
March 2019
2 Reads
2.347 Impact Factor

Kidney graft failure induced by BKPyV replication despite a strong reduction of the immunosuppressive therapy.

J Med Virol 2019 Mar 13. Epub 2019 Mar 13.

Department of Integrated Care Processes, Laboratory of Clinical Microbiology and Virology, Polyclinic Tor Vergata Foundation, Rome, Italy.

BKPyV replication is a risk factor for the development of polyomavirus-associated nephropathy in kidney transplant recipients. Here, the case of a 42 years old Caucasian patient is described who developed a kidney allograft failure because of uncontrolled BKPyV replication 7 months after transplant despite a strong reduction of the immunosuppressive therapy. The genetic analysis of the noncoding control region did not show rearrangement but two point mutations at nucleotide positions 18 and 31 within P block. Read More

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http://dx.doi.org/10.1002/jmv.25458DOI Listing

Comparison of the efficacy of sofosbuvir plus ribavirin in Chinese patients with genotype 3a or 3b HCV infection.

J Med Virol 2019 Mar 12. Epub 2019 Mar 12.

Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory for Hepatitis C and Immunotherapy for Liver Disease, Beijing, China.

Background And Aim: Genotype 3b hepatitis C virus (HCV) infection represents approximately 50% of patients with genotype 3 in China. We compared the efficacy of sofosbuvir (SOF) plus ribavirin (RBV) in Chinese patients with genotype 3a and 3b HCV.

Methods: The analyzed data are from a phase 3, open-label study of SOF plus RBV for 24 weeks conducted in China. Read More

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http://doi.wiley.com/10.1002/jmv.25454
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http://dx.doi.org/10.1002/jmv.25454DOI Listing
March 2019
6 Reads
2.347 Impact Factor

A direct comparison of four high-risk human papillomavirus tests versus the cobas test: Detecting CIN2+ in low-resource settings.

J Med Virol 2019 Mar 10. Epub 2019 Mar 10.

Department of Women's Health Care, Beijing Obstetrics and Gynecology Hospital, Capital Medical University Beijing Maternal and Child Health Care Hospital, Beijing, China.

Low-cost, accurate high-risk human papillomavirus (HR-HPV) tests are needed for cervical cancer screening in limited-resource settings. More than 200 cervical cytological specimens from hospital patients were collected and analyzed for a real-world study. We evaluated the analytical and clinical performance of four widely used HR-HPV test (Tellgen, Hybribio, Liferiver, and Sansure) based on real-time polymerase chain reaction technology platforms, compared with the cobas test. Read More

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http://dx.doi.org/10.1002/jmv.25451DOI Listing
March 2019
2.347 Impact Factor

A signal amplification system on a lateral flow immunoassay detecting for hepatitis e-antigen in human blood samples.

J Med Virol 2019 Mar 9. Epub 2019 Mar 9.

Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.

Hepatitis B e-antigen (HBeAg) is the secretory form of the nucleocapsid of the hepatitis B virus (HBV), which is a marker of viral replication. In this study, a novel signal amplification system (SAS) based on the lateral flow immunoassay (LFIA) was used for rapid detection of HBeAg in blood samples from patients or blood donors. In this assay, the detection antibody was conjugated with gold nanoparticles (GNPs), and the capture antibody was labeled with biotin. Read More

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http://dx.doi.org/10.1002/jmv.25452DOI Listing
March 2019
10 Reads
2.347 Impact Factor

Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil.

J Med Virol 2019 Mar 9. Epub 2019 Mar 9.

Laboratory of Molecular Biology, Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

Usually transmitted via respiratory droplets, parvovirus B19 (B19V) can also be acquired by blood transfusion especially because of viral persistence, resistance to blood treatment procedures, and high viral load during the early infection phase. This is particularly problematic in immunocompromised or anemic patients where the infection can have a severe outcome. As B19V DNA was detected in blood donations from South Brazil during a viral metagenomic survey performed by Next-Generation Sequencing, the objective of this retrospective study was to evaluate the seroprevalence, B19V DNA presence and circulating genotypes in a Hospital Blood Transfusion Service in Santa Maria city in South Brazil (Rio Grande do Sul state). Read More

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http://dx.doi.org/10.1002/jmv.25453DOI Listing
March 2019
2 Reads

Epidemiological description, case-fatality rate, and trends of Hantavirus Pulmonary Syndrome: 9 years of surveillance in Argentina.

J Med Virol 2019 Mar 6. Epub 2019 Mar 6.

Laboratorio Nacional de Referencia para Hantavirus, Instituto Nacional de Enfermedades Infecciosas (INEI) Administración Nacional de Laboratorio e Institutos de Salud (ANLIS) "Dr. C. G. Malbrán", Ciudad Autónoma de Buenos Aires, Argentina.

Hantavirus pulmonary syndrome (HPS) is an endemic disease in Argentina, one of the most affected countries in the Americas. Andes virus (ANDV) is the main Orthohantavirus species causing HPS in Argentina. In this study, the geographical distribution, clinical presentation, and epidemiological features of HPS from all endemic regions of Argentina were analyzed. Read More

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http://dx.doi.org/10.1002/jmv.25446DOI Listing
March 2019
3 Reads

Ombitasvir/paritaprevir/ritonavir and dasabuvir with or without sofosbuvir for patients with hepatitis C virus genotype 1 infection who failed a prior course of direct-acting antiviral therapy.

J Med Virol 2019 Mar 6. Epub 2019 Mar 6.

GastroOne, Germantown, Tennessee.

Introduction: Despite high efficacy of current direct-acting antiviral agents (DAAs) in treating chronic hepatitis C virus (HCV) infection, a small portion of patients fail treatment. QUARTZ-I was a phase 2, open-label, multicenter, two-part study that assessed the safety and efficacy of ombitasvir/paritaprevir/ritonavir (OBV/PTV/r) with dasabuvir (DSV) with or without the addition of sofosbuvir (SOF) and/or ribavirin (RBV) in DAA treatment-experienced adults with chronic HCV GT1 infection.

Materials And Methods: Genotype 1 HCV-infected patients with or without compensated cirrhosis had prior treatment failure to any DAA (part 1) or ledipasvir/SOF (part 2). Read More

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http://dx.doi.org/10.1002/jmv.25448DOI Listing
March 2019
4 Reads

Parvovirus B19 in South African blood donors.

J Med Virol 2019 Mar 6. Epub 2019 Mar 6.

Department of Virology, National Health Laboratory Service and University of KwaZulu-Natal, Durban, KwaZulu-Natal, South Africa.

Background: Parvovirus B19 (PVB19) is transmitted via transfusion of blood and blood products. PVB19 is resistant to viral inactivation methods, which poses a threat to blood safety. We investigated the prevalence of PVB19 antibodies and DNA in healthy blood donors from the South African National Blood Bank Service to evaluate the necessity of PVB19 DNA testing. Read More

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http://dx.doi.org/10.1002/jmv.25450DOI Listing
March 2019
6 Reads

Field performance of the INSTI HIV-1/-2 antibody test in two South African antenatal clinics.

J Med Virol 2019 Mar 6. Epub 2019 Mar 6.

Department of Internal Medicine, Division of Infectious Diseases, University of Pretoria, Tshwane, South Africa.

This was a prospective study that assessed field performance of the INSTI HIV-1/-2 antibody test (INSTI test) in two antenatal clinics in South Africa (SA). INSTI test was evaluated against rapid tests used at these clinics, and pooled nucleic acid amplification testing (NAAT) performed for individuals with negative rapid tests. Three hundred and eighty-six pregnant women were enrolled; 334 (86. Read More

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http://dx.doi.org/10.1002/jmv.25447DOI Listing
March 2019
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Effect of early highly active antiretroviral therapy on viral suppression among newly diagnosed men who have sex with men living with human immunodeficiency virus in Xi'an, China.

J Med Virol 2019 Mar 6. Epub 2019 Mar 6.

Department of Sexually Transmitted Diseases Prevention and Control, Xi'an Centers for Disease Control and Prevention, Xi'an, China.

Background: The number of men who have sex with men (MSM) living with human immunodeficiency virus (HIV) in China has increased rapidly and thus immediate highly active antiretroviral therapy (HAART) after diagnosis was implemented as a strategy to reduce the HIV transmission.

Methods: MSM who were diagnosed with HIV and received HAART between 2013 to 2015 in Xi'an were divided into three groups (>350, 200-350, and <200 cell/μL) according to their baseline CD4+ T cell count. The time of follow-up was calculated from the first date of receiving HAART to December 31, 2016. Read More

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http://dx.doi.org/10.1002/jmv.25449DOI Listing
March 2019
2.347 Impact Factor

First report of the genomic characterization of rubella viruses circulating in Cameroon.

J Med Virol 2019 Jun 12;91(6):928-934. Epub 2019 Mar 12.

Centre Pasteur of Cameroon, WHO National Reference Measles Laboratory, Yaoundé, Cameroon.

Rubella is an acute and contagious viral infection whose gravidity resides in infection during pregnancy, which can result in miscarriage, fetal death, stillbirth, or infants with congenital malformations. This study aimed to describe the genome of rubella viruses (RUBVs) circulating in Cameroon. Throat swabs were collected from health districts as part of the measles surveillance program from 2010 to 2016 and sent to the Centre Pasteur of Cameroon. Read More

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http://doi.wiley.com/10.1002/jmv.25445
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http://dx.doi.org/10.1002/jmv.25445DOI Listing
June 2019
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Identification of human parvovirus B19 among measles and rubella suspected patients from Cuba.

J Med Virol 2019 Feb 28. Epub 2019 Feb 28.

Department of Infection and Immunity, Infectious Diseases Research Unit, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.

Between September 2014 and December 2015, 298 sera from rash and fever patients from all over Cuba were investigated for specific IgM antibodies against measles, rubella, dengue, human parvovirus B19 (B19V) and human herpesvirus 6 (HHV6) using a commercial enzyme-linked immunosorbent assay kits. B19V IgM positive and equivocal samples were investigated by a polymerase chain reaction and genotyping. No measles, rubella or dengue cases were detected. Read More

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http://dx.doi.org/10.1002/jmv.25444DOI Listing
February 2019
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Maintaining complete virological suppression by reduced doses of nucleos(t)ide analogue in patients with chronic hepatitis B.

J Med Virol 2019 Feb 28. Epub 2019 Feb 28.

Department of Hepatogastroenterology, Liver Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

In patients with chronic hepatitis B (CHB), withdrawal of nucleos(t)ide analogues (NAs) can put patients at risk of hepatitis relapses. Here, we examined a dose-reducing strategy. From March 2008 to September 2016, 48 patients with CHB who had received full-dose NA (35 entecavir; 13 tenofovir) and achieved complete virological response (lasting for >1 year), were placed on a reduced dose of antivirals (twice a week) subsequently. Read More

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http://dx.doi.org/10.1002/jmv.25443DOI Listing
February 2019

Human adenovirus replication and persistence in hypertrophic adenoids and palatine tonsils in children.

J Med Virol 2019 Feb 28. Epub 2019 Feb 28.

Department of Cell Biology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

The role of human adenovirus (HAdV) infection in different acute diseases, such as febrile exudative tonsillitis, conjunctivitis, and pharyngoconjunctival fever is well established. However, the relationships, if any, of HAdV persistence and reactivation in the development of the chronic adenotonsillar disease is not fully understood. The present paper reports a 3-year cross-sectional hospital-based study aimed at detecting and quantifying HAdV DNA and mRNA of the HAdV hexon gene in adenoid and palatine tonsil tissues and nasopharyngeal secretions (NPS) from patients with adenotonsillar hypertrophy or recurrent adenotonsillitis. Read More

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http://doi.wiley.com/10.1002/jmv.25441
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http://dx.doi.org/10.1002/jmv.25441DOI Listing
February 2019
10 Reads