7,774 results match your criteria Journal of Medical Microbiology[Journal]


Host and pathogen factors in Klebsiella pneumoniae upper urinary tract infections in renal transplant patients.

J Med Microbiol 2019 Feb 12. Epub 2019 Feb 12.

1​Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, ul. Dębinki 7, 80-952 Gdańsk, Poland.

Purpose: To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients.

Methodology: Clinical and demographic data were registered prospectively. Phylogenetic background of K. Read More

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http://dx.doi.org/10.1099/jmm.0.000942DOI Listing
February 2019

Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses.

J Med Microbiol 2019 Feb 12. Epub 2019 Feb 12.

3 NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham, Nottingham, UK.

The liver-expressed pattern recognition receptors mannose-binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize virus entry. We observed that the human hepatoma cell line HuH7. Read More

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http://dx.doi.org/10.1099/jmm.0.000935DOI Listing
February 2019
1 Read

Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

J Med Microbiol 2019 Feb 8. Epub 2019 Feb 8.

5​Microbiology and Molecular Genetics, Michigan State University, Michigan, USA.

Purpose: Pythiosis is an infection of humans and other animals caused by the fungal-like pathogen Pythium insidiosum. This pathogen causes life-threatening infection in the infected hosts. Culture, histopathology, serology and molecular tools are used to diagnose its infections. Read More

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http://dx.doi.org/10.1099/jmm.0.000941DOI Listing
February 2019

Effects of Eclipta prostrata on gut microbiota of SAMP6 mice with osteoporosis.

J Med Microbiol 2019 Feb 8. Epub 2019 Feb 8.

1​Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Beihua South Road, Jing Hai District, Tianjin 301617, PR China.

Purpose: Traditional Chinese medicine (TCM) has been extensively studied for its preventive and treatment properties toward osteoporosis (OP). Pharmacological studies have shown that TCM Eclipta prostrata induce anti-OP effects. Considering the growing evidence demonstrating that gut microbiota (GM) is related to OP, we aimed to study the GM-dependent function and mechanism of E. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
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http://dx.doi.org/10.1099/jmm.0.000936DOI Listing
February 2019
3 Reads

New in vitro model evaluating antiseptics' efficacy in biofilm-associated Staphylococcus aureus prosthetic vascular graft infection.

J Med Microbiol 2019 Feb 8. Epub 2019 Feb 8.

1​Vilnius University Faculty of Medicine, M. K. Ciurlionio str. 21, Vilnius, Lithuania.

Purpose: To develop a new in vitro model of prosthetic vascular graft infection (PVGI) and evaluate antimicrobial and biofilm-disrupting efficacy of 0.1% octenidine dihydrochloride, 10% povidone-iodine and 0.02% chlorhexidine digluconate against biofilm-producing Staphylococcus aureus (S. Read More

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http://dx.doi.org/10.1099/jmm.0.000939DOI Listing
February 2019

Antifungal susceptibilities, biofilms, phospholipase and proteinase activities in the Candida rugosa complex and Candida pararugosa isolated from tertiary teaching hospitals.

J Med Microbiol 2019 Feb 6. Epub 2019 Feb 6.

6​Department of Biomedical Imaging, Faculty of Medicine, University Malaya Medical Centre, Kuala Lumpur, Malaysia.

Purpose: Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. Read More

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http://dx.doi.org/10.1099/jmm.0.000940DOI Listing
February 2019

Comparison of new and classical point mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from dyspeptic patients and their effects on phenotypic clarithromycin resistance.

J Med Microbiol 2019 Feb 6. Epub 2019 Feb 6.

1​Department of Medical Microbiology, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, Turkey.

Purpose: We aimed to investigate the presence of three recently identified point mutations (A2115G, G2141A and A2144T) of the 23 S rRNA gene and compare them with the three most frequently encountered point mutations (A2142G, A2142C and A2143G) in Helicobacter pylori strains in Turkey.

Methodology: A total of 63 patients (mean 47.08±12. Read More

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http://dx.doi.org/10.1099/jmm.0.000944DOI Listing
February 2019
1 Read

Heat-treated Lactobacillus plantarum increases the immune responses through activation of natural killer cells and macrophages on in vivo and in vitro models.

J Med Microbiol 2019 Feb 6. Epub 2019 Feb 6.

4​Department of Food Science & Technology and Biochip Research Center, Hoseo University, Chungnam 31499, Republic of Korea.

Purpose: Recently, Lactobacillus plantarum (nF1) has been reported to have immune-enhancing effects in an immunosuppressed-animal model. Natural killer (NK) cells and macrophages play important roles in the immune responses. However, immunomodulatory effects of heat-treated Lactobacillus plantarum-nF1 (hLp-nF1) on the activation of NK cells and macrophages have not been elucidated. Read More

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http://dx.doi.org/10.1099/jmm.0.000938DOI Listing
February 2019

Performance of bioMérieux Lowenstein-Jensen slopes in plastic tube packaging, compared to existing phenotypic methods, for efficient recovery of the Mycobacterium tuberculosis complex.

J Med Microbiol 2019 Feb 6. Epub 2019 Feb 6.

1​Section of Microbiology, Department of Laboratory Medicine, PD Hinduja Hospital and Medical Research Centre, Mumbai, India.

Purpose: Lowenstein-Jensen (LJ) medium used for cultivating Mycobacterium tuberculosis (MTB) is marketed in glass packaging. Breakage of glass slope is a major biosafety risk, especially during processing and storage, which gets magnified in large laboratories. We evaluated the performance of new bioMérieux (bMx) LJ slopes in plastic packaging, compared to bMx glass LJ medium and Becton Dickinson Mycobacterial Growth Indicator Tube (MGIT), for MTB recovery. Read More

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http://dx.doi.org/10.1099/jmm.0.000930DOI Listing
February 2019
1 Read

Characterization of intestinal Escherichia coli isolated from calves with diarrhea due to rotavirus and coronavirus.

J Med Microbiol 2019 Feb 5. Epub 2019 Feb 5.

1​Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil.

Purpose: To address more information about changes in commensal Escherichia coli during virus intestinal infection, we characterized 30 faecal E. coli isolates from calves (21 to 60 days old) with diarrhea due to rotavirus and coronavirus, which received, before diagnosis, tetracycline, gentamicin and enrofloxacin drugs.

Methodology: Clermont's phylogenetic classification; presence of genes for curli, cellulose, fimbriae (F4, F5, F6, F18, F41); and antimicrobial susceptibility were used to characterize the isolates. Read More

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http://dx.doi.org/10.1099/jmm.0.000937DOI Listing
February 2019
1 Read

Minimum 5 years' follow-up after gentamicin- and clindamycin-loaded PMMA cement in total joint arthroplasty.

J Med Microbiol 2019 Jan 31. Epub 2019 Jan 31.

1​Department of Orthopaedic Surgery, Helios ENDO-Klinik, Hamburg, Germany.

Purpose: Due to numerous advantages of the combination of clindamycin and gentamicin in polymethylmethacrylate (PMMA) cement, promising preliminary results have been reported. However, there are no data that analyse mid-term outcomes.

Methodology: This pilot study included patients who experienced 5 years of follow-up and who were treated with gentamicin- and clindamycin-loaded (G+C) PMMA cement. Read More

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http://dx.doi.org/10.1099/jmm.0.000895DOI Listing
January 2019

Whole-genome sequencing of the rare Salmonella enterica serovar Anfo isolated from food handlers.

J Med Microbiol 2019 Jan 30. Epub 2019 Jan 30.

3​Department of Laboratory Medicine, National University Hospital, Singapore.

Field investigations were conducted after a small cluster of food poisoning involving six cases was reported. While no stool samples were available from the cases for microbiological testing, Salmonella species was found to be present in the stools of food handlers with gastroenteritis symptoms. Four Salmonella isolates recovered from the food handlers were retrospectively investigated at the genome level using whole-genome sequencing (WGS). Read More

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http://dx.doi.org/10.1099/jmm.0.000934DOI Listing
January 2019
1 Read

Prevalence and genetic characterization of cephalosporin-resistant Enterobacteriaceae among dogs and cats in an animal shelter.

J Med Microbiol 2019 Jan 30. Epub 2019 Jan 30.

1​Division of Microbiology, Osaka Institute of Public Health, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.

Purpose: The prevalence of antimicrobial-resistant bacteria, especially cephalosporin-resistant Enterobacteriaceae, is a major concern for human and animal health. We investigated the prevalence of cephalosporin-resistant Enterobacteriaceae among sheltered dogs and cats with various backgrounds.

Method: Faecal samples or rectal swabs were collected from 151 dogs and 182 cats, and screened for the presence of antimicrobial-resistant bacteria. Read More

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http://dx.doi.org/10.1099/jmm.0.000933DOI Listing
January 2019
1 Read

Molecular characterization and genotyping of methicillin-resistant Staphylococcus aureus in nasal carriage of healthy Iranian children.

J Med Microbiol 2019 Jan 30. Epub 2019 Jan 30.

1​Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Purpose: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has become a considerable public health concern in both developed and developing countries due to the rapid spread of this bacterium around the world, also the epidemiology of MRSA has changed, as the isolation of MRSA strains is not limited to health-care settings or patients with predisposing risk factors. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of CA-MRSA nasal carriage in Iranian children.

Methodology: A cross-sectional study was conducted from April 2013 to March 2014. Read More

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http://dx.doi.org/10.1099/jmm.0.000924DOI Listing
January 2019
1 Read
2.248 Impact Factor

Agmatine accumulation by Pseudomonas aeruginosa clinical isolates confers antibiotic tolerance and dampens host inflammation.

J Med Microbiol 2019 Jan 28. Epub 2019 Jan 28.

1​Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455, USA.

Purpose: In the cystic fibrosis (CF) airways, Pseudomonas aeruginosa undergoes diverse physiological changes in response to inflammation, antibiotic pressure, oxidative stress and a dynamic bioavailable nutrient pool. These include loss-of-function mutations that result in reduced virulence, altered metabolism and other phenotypes that are thought to confer a selective advantage for long-term persistence. Recently, clinical isolates of P. Read More

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http://dx.doi.org/10.1099/jmm.0.000928DOI Listing
January 2019
2 Reads

The clinical significance of carbapenem-resistant Klebsiella pneumoniae rectal colonization in critically ill patients: from colonization to bloodstream infection.

J Med Microbiol 2019 Jan 28. Epub 2019 Jan 28.

7​Department of Microbiology, Aristotle University of Thessaloniki Medical School, Thessaloniki, Greece.

Purpose: To highlight the clinical significance of carbapenem-resistant Klebsiella pneumoniae (CRKP) rectal colonization by examining the risk factors for CRKP rectal colonization and subsequent bloodstream infection (BSI) in critically ill patients.

Methodology: Prospective study of CRKP rectal colonization in an intensive care unit (ICU) during a 39-month period. CRKP strains isolated from both the blood cultures and corresponding rectal specimens (n=96) of patients were screened by PCR for the presence of antibiotic resistance-associated genes. Read More

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http://dx.doi.org/10.1099/jmm.0.000921DOI Listing
January 2019
1 Read

The effects of iclaprim on exotoxin production in methicillin-resistant and vancomycin-intermediate Staphylococcus aureus.

J Med Microbiol 2019 Jan 24. Epub 2019 Jan 24.

2​Veterans Affairs Medical Center, Boise, ID, USA.

Purpose: Extracellular protein toxins contribute to the pathogenesis of Staphylococcus aureus infections. The present study compared the effects of iclaprim and trimethoprim - two folic acid synthesis inhibitors - with nafcillin and vancomycin on production of Panton-Valentine leukocidin (PVL), alpha haemolysin (AH) and toxic-shock syndrome toxin I (TSST-1) in methicillin-resistant and vancomycin-intermediate S. aureus (MRSA and VISA, respectively). Read More

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http://dx.doi.org/10.1099/jmm.0.000929DOI Listing
January 2019

Acquired qnrVC1 and blaNDM-1 resistance markers in an international high-risk Pseudomonas aeruginosa ST773 clone.

J Med Microbiol 2019 Jan 22. Epub 2019 Jan 22.

1​Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary.

A multidrug-resistant Pseudomonas aeruginosa PS1 isolated from urine clinical sample was investigated in this study. The strain exhibited resistance to piperacillin/tazobactam, ciprofloxacin, imipenem, ceftazidime but it was susceptible to colistin. Analysis of whole-genome sequencing data by ResFinder detected various resistance determinants including qnrVC1 and blaNDM-1. Read More

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http://dx.doi.org/10.1099/jmm.0.000927DOI Listing
January 2019

Genotypic and phenotypic variations in methicillin-resistant Staphylococcus aureus isolates from outpatient, inpatient and nursing homes.

J Med Microbiol 2019 Jan 22. Epub 2019 Jan 22.

2​Department of Clinical Laboratory Sciences, California State University Dominguez Hills, Carson, CA, USA.

Purpose: The epidemiological shift in MRSA distribution from healthcare-related facilities to the general population is distressing and requires continuous monitoring to manage and control the rate of incidences.

Method: The retrospective relationship between genetic and phenotypic variability of methicillin-resistant Staphylococcus aureus (MRSA) isolates was determined in respect to the specimen source, patient location, sex and age. A total of 521 MRSA isolates were classified based on SCCmec, mec, agr, pvl and spa genetic markers using three different multiplex PCRs. Read More

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http://dx.doi.org/10.1099/jmm.0.000922DOI Listing
January 2019

Synergistic activity of polymyxin B combined with vancomycin against carbapenem-resistant and polymyxin-resistant Acinetobacter baumannii: first in vitro study.

J Med Microbiol 2019 Jan 21. Epub 2019 Jan 21.

1​Universidade Estadual de Maringá, Avenida Colombo 5790, CEP 87.020-900 Maringá, Paraná, Brazil.

Purpose: The effect of a combination of polymyxin B (PMB) and vancomycin (VAN) was assessed against six Acinetobacter baumannii clinical isolates belonging to six different clusters (three PMB-susceptible and three PMB-resistant).

Methodology: The synergistic effect of the PMB-VAN combination was determined with the checkerboard, time-kill, disk-diffusion and M.I. Read More

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http://dx.doi.org/10.1099/jmm.0.000920DOI Listing
January 2019
1 Read

Evaluation of a novel immunochromatographic lateral flow assay for rapid detection of OXA-48, NDM, KPC and VIM carbapenemases in multidrug-resistant Enterobacteriaceae.

J Med Microbiol 2019 Jan 21. Epub 2019 Jan 21.

1​Microbiology/Infectious Diseases, Bioscientia Institute for Medical Diagnostics, Ingelheim, Germany.

Purpose: The global spread and increasing clinical impact of carbapenemase-producing bacteria are alarming. Rapid diagnostic techniques for carbapenemase detection are of the utmost importance to prevent delays in efficient antibiotic therapy and the control of spread in hospitals. Recently, multiplex immunochromatographic lateral flow tests (ICTs) for the fast detection of carbapenemase-producers have become commercially available. Read More

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http://dx.doi.org/10.1099/jmm.0.000925DOI Listing
January 2019

Stool PCR may not be a substitute for enrichment culture for the detection of salmonella.

J Med Microbiol 2019 Jan 21. Epub 2019 Jan 21.

1​Department of Microbiology, Level 6, The John Radcliffe Hospital, Oxford, OX3 9DU, UK.

Purpose: Polymerase chain reaction (PCR) is increasingly being used to detect enteric pathogens and is currently NICE's recommended practice. We wished to evaluate the performance characteristics of PCR for the detection of salmonella in consecutive stool samples in a real-world setting, compared to the gold standard of enrichment culture.

Methodology: We performed a prospective study over 9 months in which the PCR and culture results for salmonella were scrutinized for all stool samples sent to the laboratory. Read More

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http://dx.doi.org/10.1099/jmm.0.000923DOI Listing
January 2019

Utilizing genomic analyses to investigate the first outbreak of vanA vancomycin-resistant Enterococcus in Australia with emergence of daptomycin non-susceptibility.

J Med Microbiol 2019 Jan 21. Epub 2019 Jan 21.

1​Victorian Infectious Diseases Service, Royal Melbourne Hospital, Melbourne, Australia.

Introduction: The majority of vancomycin-resistant Enterococcus faecium (VREfm) in Australia is of the vanB genotype. An outbreak of vanA VREfm emerged in our haematology/oncology unit between November 2014 and May 2015. The first case of daptomycin non-susceptible E. Read More

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http://dx.doi.org/10.1099/jmm.0.000916DOI Listing
January 2019
1 Read

Incorporation of CD40 ligand or granulocyte-macrophage colony stimulating factor into Hantaan virus (HTNV) virus-like particles significantly enhances the long-term immunity potency against HTNV infection.

J Med Microbiol 2019 Jan 18. Epub 2019 Jan 18.

1​Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi'an, PR China.

Purpose: Hantavirus infections cause severe haemorrhagic fever with renal syndrome (HFRS) in humans and are associated with high fatality rates. In 2017, numerous outbreaks were reported in China and Germany. This represents a significant public-healthcare issue with no effective HFRS vaccines that offer a long-term immune response. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
Publisher Site
http://dx.doi.org/10.1099/jmm.0.000897DOI Listing
January 2019
6 Reads
2.248 Impact Factor

LAMP-based assay can rectify the diagnosis of Yersinia pseudotuberculosis infections otherwise missed by serology.

J Med Microbiol 2019 Feb 16;68(2):143-147. Epub 2019 Jan 16.

1​Department of Pediatrics, Kawasaki Medical School, Japan.

Background: Despite being a well-known but seldom encountered zoonotic pathogen, diagnosis of Yersinia pseudotuberculosis is not necessarily easy. Infected patients occasionally present with various symptoms resembling Kawasaki disease; thus discriminating the two in the acute phase is challenging. In addition to bacterial culture and serology, novel detection methods based on loop-mediated isothermal amplification (LAMP) are reported in the literature. Read More

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http://dx.doi.org/10.1099/jmm.0.000868DOI Listing
February 2019
1 Read

Investigation of fluoroquinolone resistance mechanism in Mycoplasma hominis isolated from urogenital samples in a Chinese hospital.

J Med Microbiol 2019 Feb 11;68(2):206-210. Epub 2019 Jan 11.

1​Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.

Purpose: Mycoplasma hominis is considered among the causes of urogenital infections and shows increasing resistance to fluoroquinolones. However, data regarding the fluoroquinolone resistance mechanism of M. hominis in Southwest China are limited. Read More

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http://dx.doi.org/10.1099/jmm.0.000913DOI Listing
February 2019

Detection and Identification of Bacterial Pathogens Directly from Sputum Samples by Pyrosequencing.

J Med Microbiol 2019 Jan 11. Epub 2019 Jan 11.

Research and Development Centre, Hangzhou D.A. Medical Laboratory, Hangzhou, Zhejiang, PR China.

Purpose: The standard culture findings for detecting and identifying bacterial pathogens in patients with lower respiratory tract infections (LRTIs) are usually not available for two to three days, which delays the initiation of appropriate antibiotic therapies. We aimed to develop a faster method of identification of bacterial pathogens in LRTIs which would offer a timelier guide to initial antibiotic choices.

Methodology: The developed PCR-pyrosequencing-based method was defined as mask PCR-pyrosequencing (MPP). Read More

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http://dx.doi.org/10.1099/jmm.0.000917DOI Listing
January 2019
2 Reads

The rise of pneumonic plague in Madagascar: current plague outbreak breaks usual seasonal mould.

J Med Microbiol 2019 Jan 11. Epub 2019 Jan 11.

6​Research and Scientific Studies Unit, College of Nursing & Allied Health Sciences, Jazan University, Jazan, Saudi Arabia.

Madagascar has just emerged from the grip of an acute urban pneumonic plague outbreak, which began in August 2017, before the usual plague season of October-April and outside the traditional plague foci in the northern and central highlands. The World Health Organization reported a total of 2417 confirmed, probable and suspected cases, including 209 deaths between 1 August and 26 November 2017. The severity and scope of this outbreak, which has affected those in higher socioeconomic groups as well as those living in poverty, along with factors including the potential for use of multi-drug-resistant strains of plague in bioterrorism, highlights the ongoing threat posed by this ancient disease. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
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http://dx.doi.org/10.1099/jmm.0.000915DOI Listing
January 2019
6 Reads

Characterization of the mechanism and impact of staphylokinase on the formation of Candida albicans and Staphylococcus aureus polymicrobial biofilms.

J Med Microbiol 2019 Jan 10. Epub 2019 Jan 10.

1​Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.

Purpose: Candida albicans and Staphylococcus aureus can be co-isolated in biofilm-associated infections. However, treatments have not been well established due to a lack of antibiofilm strategies. Hence, this study aims to characterize the mechanism and impact of Staphylokinase (Sak) on fungal-bacterial polymicrobial biofilms. Read More

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http://dx.doi.org/10.1099/jmm.0.000914DOI Listing
January 2019

Letter in Response to Evaluation of Xpert MRSA Gen 3 and BD MAX MRSA XT for meticillin-resistant Staphylococcus aureus screening in a routine diagnostic setting in a low-prevalence area. Journal of Medical Microbiology. 2017;66(1): 90-95.

J Med Microbiol 2019 Jan 10. Epub 2019 Jan 10.

1​Imperial College London, South Kensington, London. SW7 2AZ, UK.

The move towards pathology networks and hub-and-spoke models of medical laboratory service provision has significantly changed the flow of samples, and the impact of results on patients, over recent years. At the same time advances in technology, including rapid, simple to use molecular platforms, are changing the way microbiology results can be utilized. Like many other medical microbiology laboratories, we struggle with this balance for many different sample types and test requests. Read More

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http://dx.doi.org/10.1099/jmm.0.000919DOI Listing
January 2019

Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848.

J Med Microbiol 2019 Feb 10;68(2):263-278. Epub 2019 Jan 10.

1​Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.

Purpose: Burkholderia pseudomallei, the tier 1 agent of melioidosis, is a saprophytic microbe that causes endemic infections in tropical regions such as South-East Asia and Northern Australia. It is globally distributed, challenging to diagnose and treat, infectious by several routes including inhalation, and has potential for adversarial use. B. Read More

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http://dx.doi.org/10.1099/jmm.0.000908DOI Listing
February 2019

Impact of HPV infection on gene expression and methylation in oral cancer patients.

J Med Microbiol 2019 Jan 9. Epub 2019 Jan 9.

1​Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland.

Purpose: The current study aimed to examine the association between head and neck squamous cell carcinoma (HNSCC) and infection with different human papillomavirus virus (HPV) subtypes, including analysis of promoter methylation of several genes (APC, CDKN2A, MGMT, CDH1 and TIMP3) and the correlation with their mRNA expression in tumours and surgical margins.

Methodology: In 47 patients with a primary tumour of the oral cavity, HPV detection and identification of 33 subtypes was performed after previous DNA isolation using a GenoFlow HPV Array Test Kit.

Results: Fifteen patients (31. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
Publisher Site
http://dx.doi.org/10.1099/jmm.0.000898DOI Listing
January 2019
7 Reads

Clinical features and microbiological characteristics of hospital- and community-onset Escherichia coli bloodstream infection.

J Med Microbiol 2019 Feb 9;68(2):178-187. Epub 2019 Jan 9.

1​Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Clinical Laboratory, The First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.

Purpose: Escherichia coli is a leading cause of bloodstream infection (BSI) in hospitals and communities.

Methodology: We conducted a retrospective study in 2015 to evaluate the clinical features and microbiological characteristics of E. coli BSI acquired in the hospital and community. Read More

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http://dx.doi.org/10.1099/jmm.0.000904DOI Listing
February 2019

A rapid and simple detection method for phenotypic antimicrobial resistance in Escherichia coli by loop-mediated isothermal amplification.

J Med Microbiol 2019 Feb 9;68(2):169-177. Epub 2019 Jan 9.

Department of Laboratory Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Purpose: In infectious disease therapy, administration of adequate antimicrobial agents is essential for preventing the emergence and spread of resistant bacteria. However, conventional antimicrobial susceptibility testing (AST), based on bacterial growth, is time consuming; therefore, a rapid, simple assay is needed for the timely selection of appropriate antibiotics in clinical laboratories. Here, we established a simple, cost-effective, time-saving and highly sensitive AST assay based on loop-mediated isothermal amplification (LAMP). Read More

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http://dx.doi.org/10.1099/jmm.0.000903DOI Listing
February 2019
1 Read

Examining diabetic heel ulcers through an ecological lens: microbial community dynamics associated with healing and infection.

J Med Microbiol 2019 Feb 9;68(2):230-240. Epub 2019 Jan 9.

4​Foot Ulcer Trials Unit, Department of Diabetes and Endocrinology, Nottingham University Hospitals NHS Trust, Nottingham, UK.

Purpose: While some micro-organisms, such as Staphylococcus aureus, are clearly implicated in causing tissue damage in diabetic foot ulcers (DFUs), our knowledge of the contribution of the entire microbiome to clinical outcomes is limited. We profiled the microbiome of a longitudinal sample series of 28 people with diabetes and DFUs of the heel in an attempt to better characterize the relationship between healing, infection and the microbiome.

Methodology: In total, 237 samples were analysed from 28 DFUs, collected at fortnightly intervals for 6 months or until healing. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
Publisher Site
http://dx.doi.org/10.1099/jmm.0.000907DOI Listing
February 2019
10 Reads

Comparison of analytical performances of the Roche Cobas 6800 CT/NG assay with the Abbott m2000 Real Time CT/NG assay for detecting Chlamydia trachomatis and Neisseria gonorrhoeae.

J Med Microbiol 2019 Feb 3;68(2):197-200. Epub 2019 Jan 3.

1​Bacteriology Laboratory, Division of Laboratory Medicine, Department of Genetics, Laboratory Medicine and Pathology, Geneva University Hospitals, 4 rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland.

The Roche Cobas 6800 CT/NG assay was compared to the Abbott m2000 Real Time CT/NG assay for detecting Chlamydia trachomatis and Neisseria gonorrhoeae in 714 specimens referred to the bacteriology laboratory at Geneva University Hospitals, between November 2017 and March 2018, and in nine external quality controls for molecular diagnostics (seven from QCMD Glasgow and two from UK NEQAS). For C. trachomatis, the sensitivity of C6800 compared to m2000 was 100 % (95 % confidence interval [CI], 97. Read More

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http://dx.doi.org/10.1099/jmm.0.000909DOI Listing
February 2019
1 Read

Leptospirosis.

J Med Microbiol 2019 Jan 3. Epub 2019 Jan 3.

1​Respiratory and Vaccine Preventable Bacteria Reference Unit, Public Health England - National Infection Service, London, UK.

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http://dx.doi.org/10.1099/jmm.0.000899DOI Listing
January 2019

Mechanisms of ciprofloxacin resistance in Pseudomonas aeruginosa: new approaches to an old problem.

J Med Microbiol 2019 Jan;68(1):1-10

1​Department of Biochemistry, University of Otago, New Zealand.

The antibiotic ciprofloxacin is used extensively to treat a wide range of infections caused by the opportunistic pathogen Pseudomonas aeruginosa. Due to its extensive use, the proportion of ciprofloxacin-resistant P. aeruginosa isolates is rapidly increasing. Read More

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http://dx.doi.org/10.1099/jmm.0.000873DOI Listing
January 2019

Low prevalence of mupirocin resistance among Staphylococcus aureus clinical isolates from a Chinese tertiary hospital.

J Med Microbiol 2019 Feb 20;68(2):201-205. Epub 2018 Dec 20.

4​Department of Clinical Laboratory, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200001, PR China.

Between September 2013 and March 2016, 26 (1.95 %) of 1333 Staphylococcus aureus clinical isolates from a Chinese hospital were found to be resistant to mupirocin, including 18 (1.35 %) with high-level mupirocin resistance and 8 (0. Read More

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http://dx.doi.org/10.1099/jmm.0.000911DOI Listing
February 2019
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Limited efficacy of nontreponemal test combined with treponemal test in the initial syphilis screening algorithm.

J Med Microbiol 2019 Feb 20;68(2):216-220. Epub 2018 Dec 20.

1​Department of Bacteriology, Centre de Biologie Humaine, Centre Hospitalo-Universitaire Amiens Picardie, Amiens, France.

Purpose: Various syphilis screening algorithms have been proposed and are now used by many clinical laboratories following the introduction of automated treponemal tests (TTs). In France, the diagnosis of syphilis is based on a TT combined with a nontreponemal test (NTT). The objective of this study was to evaluate the diagnostic impact of NTT on initial syphilis screening at the Amiens University Hospital between January 2013 and December 2016. Read More

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http://dx.doi.org/10.1099/jmm.0.000886DOI Listing
February 2019

In vitro activity of 12 antimicrobial peptides against Mycobacterium tuberculosis and Mycobacterium avium clinical isolates.

J Med Microbiol 2019 Feb 20;68(2):211-215. Epub 2018 Dec 20.

2​Servei de Microbiologia-CDB, Hospital Clínic de Barcelona-ISGlobal, Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat deBarcelona, c/ Villarroel 170, 08036 Barcelona, Spain.

Tuberculosis (TB) remains a major threat to human health worldwide. The increasing incidence of non-tuberculous mycobacterial infections and particularly those produced by Mycobacterium avium has emphasized the need to develop new drugs. Additionally, high levels of natural drug resistance in non-tuberculous mycobacteria (NTM) and the emergence of multidrug-resistant (MDR) TB is of great concern. Read More

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http://dx.doi.org/10.1099/jmm.0.000912DOI Listing
February 2019
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Rapid detection of extra-intestinal pathogenic Escherichia coli multi-locus sequence type 127 using a specific PCR assay.

J Med Microbiol 2019 Feb 18;68(2):188-196. Epub 2018 Dec 18.

1​Faculty of Medicine and Dentistry, University of Plymouth, Plymouth, UK.

Purpose: Members of the ST127 uropathogenic E. coli (UPEC) clone have a high virulence potential and are also highly virulent in insect infection models. However, strains of this lineage are reported in relatively low numbers in many studies. Read More

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http://dx.doi.org/10.1099/jmm.0.000902DOI Listing
February 2019

Subinhibitory concentrations of tedizolid potently inhibit extracellular toxin production by methicillin-sensitive and methicillin-resistant Staphylococcus aureus.

J Med Microbiol 2019 Feb 17;68(2):255-262. Epub 2018 Dec 17.

1​Infectious Diseases Section, Department of Veterans Affairs Medical Center, Boise, ID, USA.

Purpose: Potent extracellular toxins including alpha-haemolysin, Panton-Valentine leukocidin (PVL) and toxic-shock syndrome toxin 1 (TSST-1) significantly contribute to Staphylococcus aureus pathogenesis, thus, toxin suppression is a primary focus in treatment of staphylococcal disease. S. aureus maintains complex strategies to regulate toxin expression and previous data have demonstrated that subinhibitory concentrations of beta-lactam antibiotics can adversely increase S. Read More

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http://dx.doi.org/10.1099/jmm.0.000905DOI Listing
February 2019
2.248 Impact Factor

Parechovirus A3 (PeV-A3)-associated myalgia/myositis occurs irrespective of its genetic cluster: a longitudinal molecular epidemiology of PeV-A3 in Yamagata, Japan between 2003 and 2016.

J Med Microbiol 2018 Dec 17. Epub 2018 Dec 17.

1​Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, 990-0031, Japan.

No longitudinal molecular epidemiology of parechovirus A3 (PeV-A3) over a decade is available and PeV-A3-associated myalgia/myositis has been reported only in Japan. Thus, we aimed to clarify the longitudinal molecular epidemiology of PeV-A3 with a major focus on the strains detected from PeV-A3-associated myalgia/myositis cases. We performed sequence and phylogenetic analysis for the VP1 region of PeV-A3 strains in Yamagata, Japan, between 2003 and 2016. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
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http://dx.doi.org/10.1099/jmm.0.000894DOI Listing
December 2018
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Experiences in fosfomycin susceptibility testing and resistance mechanism determination in Escherichia coli from urinary tract infections in the UK.

J Med Microbiol 2019 Feb 13;68(2):161-168. Epub 2018 Dec 13.

2​Quadram Institute, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK.

Purpose: With an increase in the numbers of bacterial isolates resistant to first-line antibiotics, there has been a revival in the use of older drugs including fosfomycin with novel mechanisms of action. We aimed to investigate the prevalence and genotypic nature of fosfomycin resistance in Escherichia coli from urinary tract infections (UTIs) using the various methods available in the clinical microbiology laboratory.

Methodology: In total, 1000 culture-positive urine samples were assessed for the presence of E. Read More

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http://dx.doi.org/10.1099/jmm.0.000901DOI Listing
February 2019
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Exposure to N-chlorotaurine induces oxidative stress responses in Aspergillus fumigatus.

J Med Microbiol 2019 Feb 13;68(2):279-288. Epub 2018 Dec 13.

1​Medical Mycology Laboratory, Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland.

Purpose: The neutrophil-derived oxidant N-chlorotaurine (NCT) displays remarkable in vivo tolerability and efficacy against a range of pathogens. The aim of this study was to characterize the response of the pulmonary pathogen Aspergillus fumigatus to NCT.

Methodology: The effect of NCT on the growth and viability of A. Read More

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http://dx.doi.org/10.1099/jmm.0.000900DOI Listing
February 2019

Population Structure and Genetic Diversity of Sporothrix globosa in China According to 10 Novel Microsatellite Loci.

J Med Microbiol 2019 Feb 13;68(2):248-254. Epub 2018 Dec 13.

1​State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, PR China.

Purpose: Sporothrix globosa is the most important agent of sporotrichosis in China. The aim of this study is to investigate the population parameters of S. globosa. Read More

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http://dx.doi.org/10.1099/jmm.0.000896DOI Listing
February 2019
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Adhesion and biofilm formation by Staphylococcus aureus clinical isolates under conditions relevant to the host: relationship with macrolide resistance and clonal lineages.

J Med Microbiol 2019 Feb 12;68(2):148-160. Epub 2018 Dec 12.

4​Department of Medicine, Campus Biomedico University, Via Alvaro del Portillo 200, 00128 Rome, Italy.

Purpose: Staphylococcus aureus isolates, collected from various clinical samples, were analysed to evaluate the contribution of the genetic background of both erythromycin-resistant (ERSA) and -susceptible (ESSA) S. aureus strains to biofilm formation.

Methods: A total of 66 ESSA and 43 ERSA clinical isolates were studied for adhesiveness and biofilm formation under different atmospheres. Read More

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http://dx.doi.org/10.1099/jmm.0.000893DOI Listing
February 2019
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Antibiotic resistance of Campylobacter jejuni isolates recovered from humans with diarrhoea in Turkey.

J Med Microbiol 2019 Feb 12;68(2):136-142. Epub 2018 Dec 12.

3​Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, USA.

Purpose: This study was aimed at investigating the occurrence and genetic mechanisms of resistance to ciprofloxacin, tetracycline and erythromycin in clinical isolates of Campylobacter jejuni recovered from human cases of acute gastroenteritis in Turkey.

Methodology: MIC values of each antibiotic were determined with the epsilometer test (E-test). Resistance genes/mutations were first screened by PCR and analysed by subsequent DNA sequencing. Read More

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http://dx.doi.org/10.1099/jmm.0.000890DOI Listing
February 2019
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Identification of aquatic mycobacteria based on sequence analysis of the 16S-23S rRNA internal transcribed spacer region.

J Med Microbiol 2019 Feb 12;68(2):221-229. Epub 2018 Dec 12.

2​Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.

Purpose: Mycobacteria are common causative agents of bacterial infections in many species of freshwater and marine fish. Identification of mycobacteria to the species level based on phenotypic tests is inappropriate and time consuming. Molecular methods such as partial or entire gene sequence determination in mycobacteria have been employed to resolve these problems. Read More

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http://dx.doi.org/10.1099/jmm.0.000891DOI Listing
February 2019
1 Read