7,808 results match your criteria Journal of Medical Microbiology[Journal]


Molecular detection of Treponema pallidum subspecies pallidum in 150-year-old foetal remains, southeastern France.

J Med Microbiol 2019 Apr 17. Epub 2019 Apr 17.

1 Aix-Marseille Univ, CNRS, EFS, ADES, Marseille, France.

Purpose: Syphilis, caused by Treponema pallidum subspecies pallidum, is considered as an old disease affecting humans; traces of such infections, including congenital syphilis, are potentially identifiable in archaeological samples. The aim of this research was to perform macroscopic and molecular investigations of T. pallidum on six infant remains, buried between 1837 and 1867, from the cemetery of 'Les Crottes' in Marseille city (southeastern France). Read More

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000978DOI Listing
April 2019
1 Read

The epidemiology of Shiga toxin-producing Escherichia coli infections in the South East of England: November 2013-March 2017 and significance for clinical and public health.

J Med Microbiol 2019 Apr 17. Epub 2019 Apr 17.

4 Field Services, National Infection Service, Public Health England, London, UK.

Purpose: This study describes the epidemiology of Shiga toxin-producing Escherichia coli (STEC) infections in a population in the South East of England.

Methods: From 1 November 2013 to 31 March 2017 participating diagnostic laboratories reported Shiga toxin gene (stx) positive real-time PCR results to local public health teams. Stx positive faecal samples/isolates were referred to the Gastrointestinal Bacteria Reference Unit (GBRU) for confirmation by culture and typing by whole genome sequencing (WGS). Read More

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000970DOI Listing
April 2019
1 Read

Outcome of a screening program for the prevention of neonatal early-onset group B Streptococcus infection: a population-based cohort study in Inner Mongolia, China.

J Med Microbiol 2019 Apr 17. Epub 2019 Apr 17.

3 Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.

Purpose: Invasive early-onset group B Streptococcus infection (EOGBS) is an important cause of severe neonatal complications but study on comprehensive GBS screening is lacking in China. This study aims to investigate the outcome of a regional anterpartum screening program for EOGBS prevention and to estimate the pros and cons of a new GBS screening strategy employed.

Methods: We performed an optimized hospital strategy for GBS screening, which targeted expectant mothers (including those with preterm births) from January 2016 to December 2016 in a population-based cohort. Read More

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000976DOI Listing
April 2019
1 Read

Existence of multiple SCCmec elements in clinical isolates of methicillin-resistant Staphylococcus aureus.

J Med Microbiol 2019 Apr 17. Epub 2019 Apr 17.

1 Department of Microbiology, JIPMER, Puducherry, India.

Purpose: Methicillin-resistant Staphylococcus aureus (MRSA) can be classified into hospital-acquired MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA), based on the associated epidemiological risk factors and their SCCmec types. We therefore studied the diversity and distribution of SCCmec elements among MRSA isolates in our region and also evaluated SCCmec typing as a tool for the classification of MRSA.

Methodology: Two hundred isolates of MRSA obtained from various clinical specimens were included. Read More

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http://dx.doi.org/10.1099/jmm.0.000977DOI Listing
April 2019
2.248 Impact Factor

Inadequate diagnostics: the case to move beyond the bacilli for detection of meningitis due to Mycobacterium tuberculosis.

J Med Microbiol 2019 Apr 17. Epub 2019 Apr 17.

3 University of Minnesota, Minneapolis, MN, USA.

Tuberculosis (TB) meningitis is extremely difficult to diagnose due to its pauci-bacillary disease nature and new techniques are needed. Improved test sensitivity would allow for greater clinician confidence in diagnostic testing and has the potential to improve patient outcomes. Traditional microbiologic and molecular tests for TB meningitis focus on detection of TB bacilli and are inadequate. Read More

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http://dx.doi.org/10.1099/jmm.0.000975DOI Listing
April 2019
1 Read

The potential of fosfomycin for multi-drug resistant sepsis: an analysis of in vitro activity against invasive paediatric Gram-negative bacteria.

J Med Microbiol 2019 Apr 17. Epub 2019 Apr 17.

2 KEMRI-Wellcome Trust Research Programme, Kilifi 80108, Kenya.

Purpose: Antimicrobial resistance (AMR) is of increasing global concern, threatening to undermine recent progress in reducing child and neonatal mortality. Repurposing older antimicrobials is a prominent strategy to combat multidrug-resistant sepsis. A potential agent is fosfomycin, however, there is scarce data regarding its in vitro activity and pharmacokinetics in the paediatric population. Read More

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http://dx.doi.org/10.1099/jmm.0.000973DOI Listing
April 2019
1 Read
2.248 Impact Factor

Improvement in serological diagnosis of pertussis by external quality assessment.

J Med Microbiol 2019 Apr 16. Epub 2019 Apr 16.

5 Department of Medical Microbiology, Capital Medical University, No.10 Xi Tou Tiao, You'an Men Wai, Feng Tai District, 100069 Beijing, PR China.

Purpose: Serological analysis is an essential tool for the diagnosis of pertussis or whooping cough, disease surveillance and the evaluation of vaccine effectiveness against Bordetella pertussis. Accurate measurement of anti-pertussis toxin (anti-PT) IgG antibody levels in sera is essential. These measurements are usually performed using immunological methods such as ELISA and multiplex immunoassays. Read More

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http://dx.doi.org/10.1099/jmm.0.000926DOI Listing

Synthesis of conjugated PIA-rSesC and immunological evaluation against biofilm-forming Staphylococcuse epidermidis.

J Med Microbiol 2019 Apr 16. Epub 2019 Apr 16.

5 Laboratory of Medical Microbiology, Department of Medical Diagnostic Sciences, KU Leuven, UZ Gasthuisberg, Herestraat 49 CDG 8th floor, B-3000 Leuven, Belgium.

Purpose: Staphylococcus epidermidis is an opportunistic pathogen and a leading cause of morbidity and premature mortality in patients with medical device-related infections, which is a concern in hospitalized patients. Developing a strategy to raise opsonic antibodies against polysaccharide intracellular adhesin (PIA) could be promising for the elimination of colonizing and biofilm-forming S. epidermidis. Read More

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http://dx.doi.org/10.1099/jmm.0.000910DOI Listing

Integrons and antibiotic resistance genes in water-borne pathogens: threat detection and risk assessment.

J Med Microbiol 2019 Apr 16. Epub 2019 Apr 16.

1 Medical Microbiology and Bioprocess Technology Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001, India.

Antibiotic-resistant genes (ARGs) are regarded as emerging environmental pollutants and pose a serious health risk to the human population. Integrons are genetic elements that are involved in the spread of ARGs amongst bacterial species. They also act as reservoirs of these resistance traits, further contributing to the development of multi-drug resistance in several water-borne pathogens. Read More

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http://dx.doi.org/10.1099/jmm.0.000972DOI Listing

Identifying indoor air Penicillium species: a challenge for allergic patients.

J Med Microbiol 2019 Apr 16. Epub 2019 Apr 16.

2 Department of Parasitology Mycology, University Hospital of Besançon, Besançon, France.

Purpose: Penicillium is the most common mould isolated in housing. Penicillium chrysogenum is the only species tested by prick test or serology for allergic patients. The American Institute of Medicine has accepted Penicillium as an aetiological agent of rhinitis in children and adults and as an asthma agent in children. Read More

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http://dx.doi.org/10.1099/jmm.0.000960DOI Listing
April 2019
3 Reads

Clinical evaluation and cost analysis of a Trioplex real-time PCR assay for the detection and differentiation of herpes simplex virus 1 and 2 in cutaneous and mucocutaneous lesions.

J Med Microbiol 2019 Apr 2. Epub 2019 Apr 2.

1 City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA.

Purpose: Herpes simplex virus (HSV) is a common lifelong sexually transmitted infection. HSV-1 typically manifests as oral cold sores, while HSV-2 is more traditionally associated with sexual transmission and infection. We have developed a real-time PCR (Trioplex) for the simultaneous detection of HSV-1 and -2 and the bacterial phage internal control (IC) MS2. Read More

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000971DOI Listing
April 2019
7 Reads

In praise of preprints.

J Med Microbiol 2019 Apr 2;68(4):503-505. Epub 2019 Apr 2.

3 Microbiology Society, London, UK.

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000954DOI Listing
April 2019
2 Reads

Polyclonal spread of multiple genotypes of Mycoplasma pneumoniae in semi-closed settings in Yamagata, Japan.

J Med Microbiol 2019 Apr 1. Epub 2019 Apr 1.

2 Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata 990-9585, Japan.

Purpose: To clarify the spread of Mycoplasma pneumoniae infections in semi-closed settings such as schools and family homes using molecular typing methods.

Methodology: We retrospectively searched for school- and family-based clusters of M. pneumoniae infections based on information regarding patients from whom M. Read More

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http://dx.doi.org/10.1099/jmm.0.000969DOI Listing

Molecular characterization and antifungal susceptibility testing of Candida nivariensis from blood samples - an Iranian multicentre study and a review of the literature.

J Med Microbiol 2019 Mar 29. Epub 2019 Mar 29.

13 Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.

Purpose: Identification of the emerging yeast species Candida nivariensis among presumptively identified Iranian Candida glabrata isolates.

Methodology: Clinical C. glabrata species complex isolates from blood (n=100; 46. Read More

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http://dx.doi.org/10.1099/jmm.0.000963DOI Listing

Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria.

J Med Microbiol 2019 Mar 27. Epub 2019 Mar 27.

1 Department of Microbiology and Parasitology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

Objective: The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis and Helichrysum italicum essential oils (EO).

Methods: Antimycobacterial potential was tested against Mycobacterium avium and Mycobacterium intracellulare using broth and water dilution method and checkerboard synergy method. Antiadhesion and antibiofilm effect of EOs was evaluated on biotic (HeLa cells) and abiotic surface (polystyrene). Read More

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http://dx.doi.org/10.1099/jmm.0.000962DOI Listing
March 2019
2 Reads

Epidemiology and susceptibility profile to classic antifungals and over-the-counter products of Malassezia clinical isolates from a Portuguese University Hospital: a prospective study.

J Med Microbiol 2019 Mar 25. Epub 2019 Mar 25.

5 Burn Unit, Department of Plastic and Reconstructive Surgery, Centro Hospitalar S. João EPE, Porto, Portugal.

Purpose: Clinical epidemiological data about the distinct Malassezia species remain scarce. The recurrence of Malassezia-related skin diseases, despite long-term use of antifungals, raises concern about the hypothetical emergence of antifungal resistance. We aimed to assess the distribution of Malassezia species among patients from a University Hospital with pityriasis versicolor, seborrheic dermatitis and healthy volunteers, and to evaluate the susceptibility profile to classic antifungals and over-the-counter compounds, searching for clinical associations. Read More

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http://dx.doi.org/10.1099/jmm.0.000966DOI Listing

The prp4 kinase gene and related spliceosome factor genes in Trichophyton rubrum respond to nutrients and antifungals.

J Med Microbiol 2019 Apr 22;68(4):591-599. Epub 2019 Mar 22.

1 Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

Purpose: Trichophyton rubrum is a dermatophyte that causes most human superficial mycoses worldwide. The spliceosome, a large ribonucleoprotein complex responsible for pre-mRNA processing, may confer adaptive advantages to deal with different stresses. Here, we assessed the structural aspects of the Prp4 kinase protein and other pre-mRNA-splicing factors (Prps) in T. Read More

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http://dx.doi.org/10.1099/jmm.0.000967DOI Listing

Genomic profile of Brazilian methicillin-resistant Staphylococcus aureus resembles clones dispersed worldwide.

J Med Microbiol 2019 Mar 22. Epub 2019 Mar 22.

3 Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, 1374 Professor Lineu Prestes Avenue, Sao Paulo, 05508-900, São Paulo State, Brazil.

Purpose: Comparative genomic analysis of strains may help us to better understand the wide diversity of their genetic profiles. The aim of this study was to analyse the genomic features of the resistome and virulome of Brazilian first methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to other Brazilian and international MRSA strains.

Methodology: The whole genomes of three MRSA strains previously isolated in Vitória da Conquista were sequenced, assembled, annotated and compared with other MRSA genomes. Read More

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http://dx.doi.org/10.1099/jmm.0.000956DOI Listing

Identification and typing of Yersinia enterocolitica and Yersinia pseudotuberculosis isolated from human clinical specimens in England between 2004 and 2018.

J Med Microbiol 2019 Apr 19;68(4):538-548. Epub 2019 Mar 19.

1 National Infection Services, Public Health England, 61 Colindale Avenue, London NW9 5HT, UK.

PurposeandMethodology. Epidemiological and microbiological data on Yersinia enterocolitica (n=699) and Yersinia pseudotuberculosis (n=35) isolated from human clinical specimens in England between April 2004 and March 2018 were reviewed. Traditional biochemical species identification and serological typing results were compared with species identifications and serotypes derived from whole-genome sequencing (WGS) data for a sub-set of these isolates (n=179). Read More

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http://dx.doi.org/10.1099/jmm.0.000943DOI Listing
April 2019
1 Read

Allicin causes fragmentation of the peptidoglycan coat in Staphylococcus aureus by effecting synthesis and aiding hydrolysis: a determination by MALDI-TOF mass spectrometry on whole cells.

J Med Microbiol 2019 Apr 19;68(4):667-677. Epub 2019 Mar 19.

1 School of Health, Sport and Bioscience, University of East London, Water Lane, London E15 4LZ, UK.

Purpose: To determine the effect of allicin on Staphylococcus aureus cell wall peptidoglycans by the application of MALDI-TOF mass spectrometry on whole cells and to relate this to current knowledge of wall-processing enzymes.

Methodology: Two different S. aureus strains were grown for 48 h after which period each culture was split into two, one part was then treated with sub-inhibitory levels of allicin while the other part left untreated as a control. Read More

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000950DOI Listing
April 2019
11 Reads

In vitro activities of daptomycin combined with fosfomycin or rifampin on planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis.

J Med Microbiol 2019 Mar;68(3):493-502

1 Department of Infectious Diseases and the Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen 518052, PR China.

Purpose: This study aimed to explore daptomycin combined with fosfomycin or rifampin against the planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis.

Methodology: Four linezolid-resistant and four linezolid-sensitive isolates of E. faecalis which formed biofilms were collected for this study. Read More

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http://dx.doi.org/10.1099/jmm.0.000945DOI Listing
March 2019
1 Read

Inhibition effect of Zedoary turmeric oil on Listeria monocytogenes and Staphylococcus aureus growth and exotoxin proteins production.

J Med Microbiol 2019 Apr 18;68(4):657-666. Epub 2019 Mar 18.

1 Department of Food Quality and Safety, College of Food Science and Engineering, Tonghua Normal University, 134000 Tonghua, PR China.

Purpose: Zedoary turmeric oil (ZTO), the steam extract of Curcuma zedoaria Rosc was researched for its chemical composition, antibacterial activity, and mechanism for countering two major food-borne pathogenic species, Listeria monocytogenes and Staphylococcus aureus.

Methodology: Gas chromatography-mass spectrometry (GC-MS) was used to analyse and characterize the chemical composition of ZTO. Its MICs for the two bacterial species and growth curves were measured. Read More

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https://www.microbiologyresearch.org/content/journal/jmm/10.
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http://dx.doi.org/10.1099/jmm.0.000949DOI Listing
April 2019
2 Reads

Animal models of orthopaedic infections. A review of rabbit models used to induce long bone bacterial infections.

J Med Microbiol 2019 Apr 15;68(4):506-537. Epub 2019 Mar 15.

3 Cell and Tissue Engineering Laboratory, IRCCS Istituto Ortopedico Galeazzi, Milano, Italy.

The development of infections is one of the main complications in orthopaedics, especially in the presence of implants for the osteosynthesis of compound fractures and joint prosthesis. Indeed, foreign materials and implants act as substrates for the adhesion and proliferation of bacterial strains able to produce biofilm, causing peri-implant osteomyelitis. The eradication of biofilm remains a great challenge for the host immune system, as well as for medical and surgical approaches, thus imposing the need for new prophylactic and/or therapeutic strategies in which animal models have an essential role. Read More

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http://dx.doi.org/10.1099/jmm.0.000952DOI Listing

Oral microbial dysbiosis in patients with Kostmann syndrome.

J Med Microbiol 2019 Apr 15;68(4):609-615. Epub 2019 Mar 15.

1 Department of Basic Sciences, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.

Purpose: The oral microbiome is maintained by host- and microbe-derived factors. A shift in microbial composition, as a result of diseases related to the immune system, is the most important step in the development of oral and dental diseases. The aim of this study was to investigate the oral microbial composition of patients with Kostmann syndrome, who have severe neutropenia, compared with healthy children. Read More

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http://dx.doi.org/10.1099/jmm.0.000964DOI Listing
April 2019
1 Read

In vitro assessment of gentamicin and azithromycin-based combination therapy against Neisseria gonorrhoeae isolates in India.

J Med Microbiol 2019 Apr 14;68(4):555-559. Epub 2019 Mar 14.

2 Department of Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi, India.

Purpose: The public health burden of infections caused by Neisseria gonorrhoeae is magnified due to high rates of resistance to traditional antimicrobials. The aim of this study was to evaluate the in vitro efficacy of an alternative dual therapy comprising gentamicin and azithromycin.

Methodology: The E-test method was used to determine the minimum inhibitory concentrations (MICs) of gentamicin and azithromycin individually prior to testing in combination using the cross or 90 angle formation method. Read More

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http://dx.doi.org/10.1099/jmm.0.000953DOI Listing
April 2019
1 Read

Phenotypic and molecular detection methods for carbapenemase-producing organisms and their clinical significance at two Scottish tertiary care hospitals.

J Med Microbiol 2019 Apr 14;68(4):560-565. Epub 2019 Mar 14.

1 Clinical Microbiology, Laboratory Medicine, Royal Infirmary of Edinburgh, Edinburgh, UK.

Purpose: This study evaluated in-house PCR testing for local identification of bacteria carrying the major carbapenemase genes (blaOXA-48-like, blaVIM, blaNDM, blaKPC and blaIMP).

Methodology: Carbapenemase-producing organisms (CPOs) isolated from patients managed in two tertiary care hospitals in Scotland from September 2014-January 2017 were investigated. A combination of chromogenic screening agar (ChromID CARBA SMART), a carbapenem hydrolysis test (Rapidec Carba NP) and in-house real-time PCR for the blaOXA-48-like, blaVIM, blaNDM, blaKPC and blaIMP genes were utilized. Read More

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http://dx.doi.org/10.1099/jmm.0.000931DOI Listing

Oral streptococci show diversity in resistance to complement immunity.

J Med Microbiol 2019 Apr 7;68(4):600-608. Epub 2019 Mar 7.

1 Department of Oral Diagnosis, Piracicaba Dental School - State University of Campinas, Piracicaba, SP, Brazil.

Purpose: Mechanisms underlying systemic infections by oral species of Mitis (Streptococcus mitis, Streptococcus oralis) and Sanguinis (Streptococcus gordonii, Streptococcus sanguinis) commensal streptococci are poorly understood. This study investigates profiles of susceptibility to complement-mediated host immunity in representative strains of these four species, which were isolated from oral sites or from the bloodstream.

Methodology: Deposition of complement opsonins (C3b/iC3b), and surface binding to C-reactive protein (CRP) and to IgG antibodies were quantified by flow cytometry in 34 strains treated with human serum (HS), and compared to rates of opsonophagocytosis by human PMN mediated by complement (CR1/3) and/or IgG Fc (FcγRII/III) receptors. Read More

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http://dx.doi.org/10.1099/jmm.0.000955DOI Listing

Respiratory syncytial virus promoted the differentiation of Th17 cells in airway microenvironment through activation of Notch-1/Delta3.

J Med Microbiol 2019 Apr 7;68(4):649-656. Epub 2019 Mar 7.

1 Respiratory Department, Xiangya Hospital, Central South University, Changsha 410008, Hunan, PR China.

Background: Respiratory syncytial virus (RSV) infection is associated with serious lung disease in infants and immunocompromised individuals and is linked to development of asthma. Infection of RSV has been shown to induce Th lymphocyte differentiation. The present study was designed to determine the effects of RSV on the expression of Notch-1 and the related mechanisms on subsequent differentiation of Th lymphocytes. Read More

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http://dx.doi.org/10.1099/jmm.0.000959DOI Listing

Low human parvovirus B19 (B19V) DNA prevalence in blood donors from Central-West Brazil.

J Med Microbiol 2019 Apr 7;68(4):622-626. Epub 2019 Mar 7.

3 Faculty of Ceilândia, University of Brasilia, Federal District, Brazil.

Parvovirus B19 (B19V) transmission may occur through blood transfusion as a result of asymptomatic viral persistence in blood donors. Our study evaluated the prevalence and viral load of B19V in blood donors from Brasilia, Federal District, Central-West Brazil. B19V DNA detection and quantification were performed in 477 blood donors. Read More

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http://dx.doi.org/10.1099/jmm.0.000957DOI Listing

The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock.

J Med Microbiol 2019 Apr 6;68(4):627-632. Epub 2019 Mar 6.

1 Animal Research Centre, University of the Sunshine Coast, Sippy Downs, QLD, Australia.

Chlamydia pecorum and Chlamydia abortus are related ruminant pathogens endemic to different global regions. Potential co-infections combined with the lack of species-specific serological assays challenge accurate diagnosis. Serological screening revealed low C. Read More

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http://dx.doi.org/10.1099/jmm.0.000951DOI Listing

Whole-genome-based analysis reveals multiclone Serratia marcescens outbreaks in a non-Neonatal Intensive Care Unit setting in a tertiary care hospital in India.

J Med Microbiol 2019 Apr 6;68(4):616-621. Epub 2019 Mar 6.

3 Department of Periodontology and Restorative Dentistry, Muenster University Hospital, Waldeyerstrasse 30, 48149 Muenster, Germany.

We report the use of next generation sequencing (NGS) for investigating an outbreak of 13 cases of Serratia marcescens blood stream infections in a non-Neonatal Intensive Care Unit (non-NICU) setting in a tertiary care hospital in India over 5 months. Thirteen cases of sepsis due to S. marcescens were identified in various Intensive Care Units (ICUs) over 5 months. Read More

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http://dx.doi.org/10.1099/jmm.0.000947DOI Listing
April 2019
2 Reads
2.248 Impact Factor

Enhanced infectivity of strains of Helicobacter pylori isolated from children compared with parental strains.

J Med Microbiol 2019 Apr 26;68(4):633-641. Epub 2019 Feb 26.

1 Department of Infectious Diseases, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.

Purpose: Intra-familial infection, mother-to-child infection, is considered to be one of the main routes of transmission for Helicobacter pylori, in developed countries such as Japan. A major role for intra-familial spread in the pathogenicity of H. pylori is now beyond controversy, although the major route of transmission remains poorly understood. Read More

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http://dx.doi.org/10.1099/jmm.0.000918DOI Listing
April 2019
1 Read

Micro-organisms attached to the lumens and balloons of indwelling urinary catheters and correlation with symptoms, antibiotic use and catheter specimen of urine results.

J Med Microbiol 2019 Apr 26;68(4):549-554. Epub 2019 Feb 26.

1 Biomaterials-Related Infection Group, School of Medicine, University of Nottingham, Nottingham, UK.

To determine micro-organisms attached to removed urethral catheters and relate this to patient-specific information. Indwelling urethral catheters were collected from patients at a UK teaching hospital. The balloon and lumen were sonicated, and micro-organisms were enumerated. Read More

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http://dx.doi.org/10.1099/jmm.0.000946DOI Listing
April 2019
2 Reads

Clinical and microbial epidemiology of otomycosis in the city of Yasuj, southwest Iran, revealing Aspergillus tubingensis as the dominant causative agent.

J Med Microbiol 2019 Apr 25;68(4):585-590. Epub 2019 Feb 25.

1 Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

Purpose: Otomycosis is a mycotic infection of the external auditory canal and can be caused by a wide range of fungal species. In this study, we aimed to identify fungal isolates from patients suspected of otomycosis.

Methodology: External ear canal samples were taken from patients referred to the outpatient department of Shahid-Mofatteh Clinic in the city of Yasuj, Iran, and examined by direct microscopy and culture. Read More

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http://dx.doi.org/10.1099/jmm.0.000948DOI Listing
April 2019
2.248 Impact Factor

Host and pathogen factors in Klebsiella pneumoniae upper urinary tract infections in renal transplant patients.

J Med Microbiol 2019 Mar 12;68(3):382-394. Epub 2019 Feb 12.

1 Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, ul. Dębinki 7, 80-952 Gdańsk, Poland.

Purpose: To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients.

Methodology: Clinical and demographic data were registered prospectively. Phylogenetic background of K. Read More

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http://dx.doi.org/10.1099/jmm.0.000942DOI Listing
March 2019
1 Read

Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses.

J Med Microbiol 2019 Apr 12;68(4):642-648. Epub 2019 Feb 12.

1 School of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, UK.

The liver-expressed pattern recognition receptors mannose-binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize virus entry. We observed that the human hepatoma cell line HuH7. Read More

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http://dx.doi.org/10.1099/jmm.0.000935DOI Listing
April 2019
2 Reads

Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

J Med Microbiol 2019 Apr 8;68(4):574-584. Epub 2019 Feb 8.

3 Biomedical Laboratory Diagnostics, Michigan State University, Michigan, USA.

Purpose: Pythiosis is an infection of humans and other animals caused by the fungal-like pathogen Pythium insidiosum. This pathogen causes life-threatening infection in the infected hosts. Culture, histopathology, serology and molecular tools are used to diagnose its infections. Read More

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http://dx.doi.org/10.1099/jmm.0.000941DOI Listing

Effects of Eclipta prostrata on gut microbiota of SAMP6 mice with osteoporosis.

J Med Microbiol 2019 Mar 8;68(3):402-416. Epub 2019 Feb 8.

1 Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Beihua South Road, Jing Hai District, Tianjin 301617, PR China.

Purpose: Traditional Chinese medicine (TCM) has been extensively studied for its preventive and treatment properties toward osteoporosis (OP). Pharmacological studies have shown that TCM Eclipta prostrata induce anti-OP effects. Considering the growing evidence demonstrating that gut microbiota (GM) is related to OP, we aimed to study the GM-dependent function and mechanism of E. Read More

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http://www.microbiologyresearch.org/content/journal/jmm/10.1
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http://dx.doi.org/10.1099/jmm.0.000936DOI Listing
March 2019
12 Reads

New in vitro model evaluating antiseptics' efficacy in biofilm-associated Staphylococcus aureus prosthetic vascular graft infection.

J Med Microbiol 2019 Mar 8;68(3):432-439. Epub 2019 Feb 8.

1 Vilnius University Faculty of Medicine, M. K. Ciurlionio str. 21, Vilnius, Lithuania.

Purpose: To develop a new in vitro model of prosthetic vascular graft infection (PVGI) and evaluate antimicrobial and biofilm-disrupting efficacy of 0.1% octenidine dihydrochloride, 10% povidone-iodine and 0.02% chlorhexidine digluconate against biofilm-producing Staphylococcus aureus (S. Read More

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http://dx.doi.org/10.1099/jmm.0.000939DOI Listing
March 2019
3 Reads

Antifungal susceptibilities, biofilms, phospholipase and proteinase activities in the Candida rugosa complex and Candida pararugosa isolated from tertiary teaching hospitals.

J Med Microbiol 2019 Mar 6;68(3):346-354. Epub 2019 Feb 6.

6 Department of Biomedical Imaging, Faculty of Medicine, University Malaya Medical Centre, Kuala Lumpur, Malaysia.

Purpose: Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. Read More

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http://dx.doi.org/10.1099/jmm.0.000940DOI Listing

Comparison of new and classical point mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from dyspeptic patients and their effects on phenotypic clarithromycin resistance.

J Med Microbiol 2019 Apr 6;68(4):566-573. Epub 2019 Feb 6.

1 Department of Medical Microbiology, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, Turkey.

Purpose: We aimed to investigate the presence of three recently identified point mutations (A2115G, G2141A and A2144T) of the 23 S rRNA gene and compare them with the three most frequently encountered point mutations (A2142G, A2142C and A2143G) in Helicobacter pylori strains in Turkey.

Methodology: A total of 63 patients (mean 47.08±12. Read More

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http://dx.doi.org/10.1099/jmm.0.000944DOI Listing
April 2019
9 Reads

Heat-treated Lactobacillus plantarum increases the immune responses through activation of natural killer cells and macrophages on in vivo and in vitro models.

J Med Microbiol 2019 Mar 6;68(3):467-474. Epub 2019 Feb 6.

4 Department of Food Science & Technology and Biochip Research Center, Hoseo University, Chungnam 31499, Republic of Korea.

Purpose: Recently, Lactobacillus plantarum (nF1) has been reported to have immune-enhancing effects in an immunosuppressed-animal model. Natural killer (NK) cells and macrophages play important roles in the immune responses. However, immunomodulatory effects of heat-treated Lactobacillus plantarum-nF1 (hLp-nF1) on the activation of NK cells and macrophages have not been elucidated. Read More

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http://dx.doi.org/10.1099/jmm.0.000938DOI Listing

Performance of bioMérieux Lowenstein-Jensen slopes in plastic tube packaging, compared to existing phenotypic methods, for efficient recovery of the Mycobacterium tuberculosis complex.

J Med Microbiol 2019 Mar 6;68(3):398-401. Epub 2019 Feb 6.

1 Section of Microbiology, Department of Laboratory Medicine, PD Hinduja Hospital and Medical Research Centre, Mumbai, India.

Purpose: Lowenstein-Jensen (LJ) medium used for cultivating Mycobacterium tuberculosis (MTB) is marketed in glass packaging. Breakage of glass slope is a major biosafety risk, especially during processing and storage, which gets magnified in large laboratories. We evaluated the performance of new bioMérieux (bMx) LJ slopes in plastic packaging, compared to bMx glass LJ medium and Becton Dickinson Mycobacterial Growth Indicator Tube (MGIT), for MTB recovery. Read More

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http://dx.doi.org/10.1099/jmm.0.000930DOI Listing
March 2019
4 Reads

Characterization of intestinal Escherichia coli isolated from calves with diarrhea due to rotavirus and coronavirus.

J Med Microbiol 2019 Mar 5;68(3):417-423. Epub 2019 Feb 5.

1 Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil.

Purpose: To address more information about changes in commensal Escherichia coli during virus intestinal infection, we characterized 30 faecal E. coli isolates from calves (21 to 60 days old) with diarrhea due to rotavirus and coronavirus, which received, before diagnosis, tetracycline, gentamicin and enrofloxacin drugs.

Methodology: Clermont's phylogenetic classification; presence of genes for curli, cellulose, fimbriae (F4, F5, F6, F18, F41); and antimicrobial susceptibility were used to characterize the isolates. Read More

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http://dx.doi.org/10.1099/jmm.0.000937DOI Listing
March 2019
1 Read

Minimum 5 years' follow-up after gentamicin- and clindamycin-loaded PMMA cement in total joint arthroplasty.

J Med Microbiol 2019 Mar 31;68(3):475-479. Epub 2019 Jan 31.

1​Department of Orthopaedic Surgery, Helios ENDO-Klinik, Hamburg, Germany.

Purpose: Due to numerous advantages of the combination of clindamycin and gentamicin in polymethylmethacrylate (PMMA) cement, promising preliminary results have been reported. However, there are no data that analyse mid-term outcomes.

Methodology: This pilot study included patients who experienced 5 years of follow-up and who were treated with gentamicin- and clindamycin-loaded (G+C) PMMA cement. Read More

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http://dx.doi.org/10.1099/jmm.0.000895DOI Listing

Whole-genome sequencing of the rare Salmonella enterica serovar Anfo isolated from food handlers.

J Med Microbiol 2019 Mar 30;68(3):429-431. Epub 2019 Jan 30.

1 National Public Health Laboratory, Ministry of Health, Singapore.

Field investigations were conducted after a small cluster of food poisoning involving six cases was reported. While no stool samples were available from the cases for microbiological testing, Salmonella species was found to be present in the stools of food handlers with gastroenteritis symptoms. Four Salmonella isolates recovered from the food handlers were retrospectively investigated at the genome level using whole-genome sequencing (WGS). Read More

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http://dx.doi.org/10.1099/jmm.0.000934DOI Listing
March 2019
3 Reads

Prevalence and genetic characterization of cephalosporin-resistant Enterobacteriaceae among dogs and cats in an animal shelter.

J Med Microbiol 2019 Mar 30;68(3):339-345. Epub 2019 Jan 30.

1 Division of Microbiology, Osaka Institute of Public Health, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.

Purpose: The prevalence of antimicrobial-resistant bacteria, especially cephalosporin-resistant Enterobacteriaceae, is a major concern for human and animal health. We investigated the prevalence of cephalosporin-resistant Enterobacteriaceae among sheltered dogs and cats with various backgrounds.

Method: Faecal samples or rectal swabs were collected from 151 dogs and 182 cats, and screened for the presence of antimicrobial-resistant bacteria. Read More

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http://dx.doi.org/10.1099/jmm.0.000933DOI Listing
March 2019
1 Read

Molecular characterization and genotyping of methicillin-resistant Staphylococcus aureus in nasal carriage of healthy Iranian children.

J Med Microbiol 2019 Mar 30;68(3):374-378. Epub 2019 Jan 30.

1 Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Purpose: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has become a considerable public health concern in both developed and developing countries due to the rapid spread of this bacterium around the world, also the epidemiology of MRSA has changed, as the isolation of MRSA strains is not limited to health-care settings or patients with predisposing risk factors. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of CA-MRSA nasal carriage in Iranian children.

Methodology: A cross-sectional study was conducted from April 2013 to March 2014. Read More

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http://dx.doi.org/10.1099/jmm.0.000924DOI Listing
March 2019
1 Read
2.248 Impact Factor

Agmatine accumulation by Pseudomonas aeruginosa clinical isolates confers antibiotic tolerance and dampens host inflammation.

J Med Microbiol 2019 Mar 22;68(3):446-455. Epub 2019 Jan 22.

1 Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455, USA.

Purpose: In the cystic fibrosis (CF) airways, Pseudomonas aeruginosa undergoes diverse physiological changes in response to inflammation, antibiotic pressure, oxidative stress and a dynamic bioavailable nutrient pool. These include loss-of-function mutations that result in reduced virulence, altered metabolism and other phenotypes that are thought to confer a selective advantage for long-term persistence. Recently, clinical isolates of P. Read More

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http://dx.doi.org/10.1099/jmm.0.000928DOI Listing
March 2019
3 Reads

The clinical significance of carbapenem-resistant Klebsiella pneumoniae rectal colonization in critically ill patients: from colonization to bloodstream infection.

J Med Microbiol 2019 Mar 30;68(3):326-335. Epub 2019 Jan 30.

7 Department of Microbiology, Aristotle University of Thessaloniki Medical School, Thessaloniki, Greece.

Purpose: To highlight the clinical significance of carbapenem-resistant Klebsiella pneumoniae (CRKP) rectal colonization by examining the risk factors for CRKP rectal colonization and subsequent bloodstream infection (BSI) in critically ill patients.

Methodology: Prospective study of CRKP rectal colonization in an intensive care unit (ICU) during a 39-month period. CRKP strains isolated from both the blood cultures and corresponding rectal specimens (n=96) of patients were screened by PCR for the presence of antibiotic resistance-associated genes. Read More

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http://dx.doi.org/10.1099/jmm.0.000921DOI Listing
March 2019
5 Reads