10,917 results match your criteria Journal of Lipid Research[Journal]


Membrane lipids and their degradation compounds control GM2 catabolism at intralysosomal luminal vesicles.

J Lipid Res 2019 Apr 15. Epub 2019 Apr 15.

University Bonn, Germany

The catabolism of ganglioside GM2 is dependent on three gene products. Mutations in any of these genes result in a different type of GM2 gangliosidosis (Tay-Sachs disease, B1 variant, Sandhoff disease and the AB-variant), with GM2 as major lysosomal storage compound. GM2 is also a secondary storage compound in lysosomal storage diseases like Niemann-Pick disease type A, B and C with primary storage of SM and cholesterol, respectively. Read More

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http://dx.doi.org/10.1194/jlr.M092551DOI Listing

Genetic and pharmacological inhibition of acid ceramidase prevents asymmetric cell division by neosis.

J Lipid Res 2019 Apr 15. Epub 2019 Apr 15.

Medical University of South Carolina, United States

Radiation treatment failure or relapse after initial response to chemotherapy present significant clinical challenges in cancer patients. Escape from initial courses of treatment can involve re-activation of embryonic developmental stages, with the formation of polynuclear giant cancer cells (PGCC). This strategy of de-differentiation can insulate cancer cells from a variety of treatments and allows a residual subpopulation to re-establish tumors after treatment. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.M092247
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http://dx.doi.org/10.1194/jlr.M092247DOI Listing
April 2019
1 Read

Acid ceramidase promotes drug resistance in acute myeloid leukemia through NF-kB-dependent P-glycoprotein upregulation.

J Lipid Res 2019 Apr 8. Epub 2019 Apr 8.

University of Virginia, United States;

Acute myeloid leukemia (AML) is the most common acute leukemia in adults. More than half of older AML patients fail to respond to cytotoxic chemotherapy, and most responders relapse with drug resistant disease. Failure to achieve complete remission can be partly attributed to the drug resistance advantage of AML blasts that frequently express P-glycoprotein (P-gp), an ATP-binding cassette transporter. Read More

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http://dx.doi.org/10.1194/jlr.M091876DOI Listing
April 2019
2 Reads

The triglyceride synthesis enzymes DGAT1 and DGAT2 have distinct and overlapping functions in adipocytes.

J Lipid Res 2019 Apr 1. Epub 2019 Apr 1.

Harvard University, United States

Mammals store metabolic energy as triacylglycerols (TG) in adipose tissue. TG synthesis is catalyzed by the evolutionarily unrelated acyl CoA:diacylglycerol acyltransferase (DGAT) enzymes, DGAT1 and DGAT2, which catalyze the same reaction and account for nearly all TG synthesis. The reasons for their convergent evolution to synthesize TG remain unclear. Read More

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http://dx.doi.org/10.1194/jlr.M093112DOI Listing

New fluorogenic probes for neutral and alkaline ceramidases.

J Lipid Res 2019 Mar 29. Epub 2019 Mar 29.

IQAC-CSIC, Spain.

New fluorogenic ceramidase substrates derived from the N acyl modification of our previously reported probes (RBM14) are reported. While none of the new probes were superior to the known RBM14C12 as acid ceramidase substrates, the corresponding nervonic acid amide (RBM14C24:1) is an efficient and selective substrate for the recombinant human neutral ceramidase, both in cell lysates and in intact cells. A second generation of substrates, incorporating the natural 2 (N acylamino) 1,3 diol 4 ene framework (compounds RBM15) is also reported. Read More

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http://dx.doi.org/10.1194/jlr.D092759DOI Listing

Lipid droplet quantification based on iterative image processing.

J Lipid Res 2019 Mar 29. Epub 2019 Mar 29.

Heidelberg University, Germany.

Lipid droplets (LDs) are ubiquitous and highly dynamic subcellular organelles required for the storage of neutral lipids. LD number and size distribution are key parameters affected not only by nutrient supply but also by lipotoxic stress and metabolic regulation. Current methods for LD quantification lack general applicability and are either based on time consuming manual evaluation or show limitations if LDs are high in numbers or closely clustered. Read More

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http://dx.doi.org/10.1194/jlr.D092841DOI Listing
March 2019
2 Reads

PNPLA2 influences secretion of triglyceride-rich lipoproteins by human hepatoma cells.

J Lipid Res 2019 Mar 27. Epub 2019 Mar 27.

Karolinska Institutet, Sweden

Patatin-like phospholipase domain containing proteins (PNPLAs) are involved in triglyceride hydrolysis and lipid-droplet homeostasis in mice, but the physiological significance of the PNPLAs for triglyceride metabolism in human hepatocytes is unclear. Here we investigate the roles of PNPLA2, PNPLA3 and PNPLA4 in triglyceride metabolism of human Huh7 and HepG2 hepatoma cells using gene-specific inhibition methods. siRNA inhibition of PNPLA3 or PNPLA4 is not associated with changes in triglyceride hydrolysis, secretion of triglyceride-rich lipoproteins (TRLs) or triglyceride accumulation. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.M090928
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http://dx.doi.org/10.1194/jlr.M090928DOI Listing
March 2019
10 Reads

PCSK7 gene variation bridges atherogenic dyslipidemia with hepatic inflammation in NAFLD patients.

J Lipid Res 2019 Mar 27. Epub 2019 Mar 27.

Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, Italy;

Dyslipidemia and altered iron metabolism are typical features of non-alcoholic fatty liver disease (NAFLD). Proprotein Convertase Subtilisin/Kexin Type 7 (PCSK7) gene variation has been associated with circulating lipids and liver damage during iron overload. Aim of this study was to examine the impact of the PCSK7 rs236918 variant on NAFLD-related traits in 1,801 individuals from the Liver Biopsy Cohort (LBC), 500,000 from the UK Biobank Cohort (UKBBC), and 4,580 from the Dallas Heart Study (DHS). Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.P090449
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http://dx.doi.org/10.1194/jlr.P090449DOI Listing
March 2019
2 Reads

Anti-parasitic drug discovery takes a giant leap forward.

Authors:
Joseph T Nickels

J Lipid Res 2019 Mar 27. Epub 2019 Mar 27.

Institute for Metabolic Disorders, United States

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http://dx.doi.org/10.1194/jlr.C094250DOI Listing
March 2019
1 Read

DHA intake interacts with ELOVL2 and ELOVL5 genetic variants to influence polyunsaturated fatty acids in human milk.

J Lipid Res 2019 Mar 26. Epub 2019 Mar 26.

Jilin University, China

Endogenous synthesis of PUFAs is mediated by genes controlling fatty acid elongases 2 and 5 ( and ) and by exogenous DHA intake. Associations between elongases and PUFA levels probably involve genetic variants of and changes in DHA intake, but data about their combined effect on PUFA levels are sparse. We hypothesized that each factor would directly affect PUFAs and that interactions between haplotypes and DHA intake would influence PUFAs. Read More

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http://dx.doi.org/10.1194/jlr.M090951DOI Listing

n-3 PUFAs improve erythrocyte fatty acid profile in patients with small abdominal aortic aneurysms: a randomised controlled trial.

J Lipid Res 2019 Mar 26. Epub 2019 Mar 26.

University of the Sunshine Coast, Australia;

Abdominal aortic aneurysm (AAA) is an important cause of death in older adults which has no current drug therapy. Inflammation and abnormal redox status are believed to be key pathogenic mechanisms for AAA. In light of evidence correlating inflammation with aberrant fatty acid profiles, this study compared erythrocyte fatty acid content in 43 AAA patients (diameter 3. Read More

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http://dx.doi.org/10.1194/jlr.P093013DOI Listing
March 2019
1 Read

Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood.

J Lipid Res 2019 Mar 22. Epub 2019 Mar 22.

Wuhan university, China.

Vitamin D metabolites analysis possessed significant clinical value for pediatric department. However, invasive venipuncture sampling and high blood consumption inflicted much suffering on the young patients. For alleviation, we carried out a LC-MS method for 25-hydroxyvitamin D quantification in only 3 μL of plasma from the considerably less invasive finger-prick blood samples. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.D092197
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http://dx.doi.org/10.1194/jlr.D092197DOI Listing
March 2019
1 Read
4.421 Impact Factor

Metabolic disease and ABHD6 alter the circulating bis(monoacylglycerol)phosphate profile in mice and humans.

J Lipid Res 2019 Mar 20. Epub 2019 Mar 20.

Institute of Molecular Biosciences, University of Graz, Austria;

Bis(monoacylglycerol)phosphate (BMP) is a phospholipid crucial for lipid degradation and sorting in acidic organelles. Genetic and drug-induced lysosomal storage disorders (LSD) are associated with increased BMP concentrations in tissues and in the circulation. Data on BMP in disorders other than LSD, however, are scarce and key enzymes regulating BMP metabolism remain elusive. Read More

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http://dx.doi.org/10.1194/jlr.M093351DOI Listing
March 2019
7 Reads
4.421 Impact Factor

AdipoR1 and AdipoR2 Maintain Membrane Fluidity in Most Human Cell Types and Independently of Adiponectin.

J Lipid Res 2019 Mar 19. Epub 2019 Mar 19.

University of Gothenburg, Sweden

The fatty acid composition of phospholipids must be tightly regulated to maintain optimal cell membrane properties and compensate for a highly variable supply of dietary fatty acids. Earlier studies have shown that AdipoR2 and its homolog PAQR-2 are important regulators of phospholipid fatty acid composition in HEK293 cells and C. elegans, respectively. Read More

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http://dx.doi.org/10.1194/jlr.M092494DOI Listing

Metabolomic correlates of central adiposity and earlier life body mass index.

J Lipid Res 2019 Mar 18. Epub 2019 Mar 18.

University College London, United Kingdom.

Body mass index (BMI) is correlated with circulating metabolites but few studies discuss other adiposity measures and little is known about metabolomic correlates of body mass index from early life. We investigated associations between different adiposity measures, BMI from childhood through adulthood, and metabolites quantified from serum using 1H NMR spectroscopy in 900 British men and women aged 60-64. We assessed BMI, waist-to-hip ratio (WHR), and android-to-gynoid fat ratio (AGR) and BMI from childhood through adulthood. Read More

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http://dx.doi.org/10.1194/jlr.P085944DOI Listing

Long And Very Long Lamellar Phases In Model Stratum Corneum Lipid Membranes.

J Lipid Res 2019 Mar 18. Epub 2019 Mar 18.

Charles University, Czech Republic.

Membrane models of the stratum corneum (SC) lipid barrier, either healthy or affected by recessive X-linked ichthyosis, constructed from ceramide (Cer; non-hydroxyacyl sphingosine CerNS24 alone or with omega-O-acylceramide CerEOS), free fatty acids (C16-24), cholesterol (Chol) and cholesteryl sulfate (CholS) were investigated. X-ray diffraction (XRD) revealed a previously unreported polymorphism of the membranes. In the absence of Cer EOS, the membranes formed a short lamellar phase (SLP; the repeat distance d = 5. Read More

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http://dx.doi.org/10.1194/jlr.M090977DOI Listing

AMP-activated protein kinase activation ameliorates eicosanoid dysregulation in high-fat-induced kidney disease in mice.

J Lipid Res 2019 Mar 12. Epub 2019 Mar 12.

UT Health San Antonio, United States.

High-fat diet (HFD) causes renal lipotoxicity that is ameliorated with AMPK activation. Although bioactive eicosanoids increase with HFD and are essential in regulation of renal disease, their role in the inflammatory response to HFD-induced kidney disease and their modulation by AMPK activation remain unexplored. In a mouse model, we explored the effects of HFD on eicosanoid synthesis and the role of AMPK activation in ameliorating these changes. Read More

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http://dx.doi.org/10.1194/jlr.M088690DOI Listing

Advances in determining signaling mechanisms of ceramide and role in disease.

J Lipid Res 2019 Mar 7. Epub 2019 Mar 7.

Stony Brook Medicine, United States

Ceramide is a critical bioactive lipid involved in diverse cellular processes. It has been proposed to regulate cellular processes by influencing membrane properties and by directly interacting with effector proteins. Advances over the past decade have improved our understanding of ceramide as a bioactive lipid. Read More

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http://dx.doi.org/10.1194/jlr.S092874DOI Listing

Overexpression of Nudt7 decreases bile acid levels and peroxisomal fatty acid oxidation in the liver.

J Lipid Res 2019 Mar 7. Epub 2019 Mar 7.

West Virginia University, United States

Lipid metabolism requires coenzyme A (CoA), an essential cofactor found in multiple subcellular compartments including the peroxisomes. In the liver, CoA levels are dynamically adjusted between the fed and fasted states. Elevated CoA levels in the fasted state are driven by increased synthesis; however, this also correlates with decreased expression of Nudt7, the major CoA-degrading enzyme in the liver. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.M092676
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http://dx.doi.org/10.1194/jlr.M092676DOI Listing
March 2019
4 Reads

Uptake and Metabolism of β-apo-8'-carotenal, β-apo-10'-carotenal, and β-apo-13-carotenone in Caco-2 cells.

J Lipid Res 2019 Mar 6. Epub 2019 Mar 6.

The Ohio State University, United States

β-Apocarotenoids are eccentric cleavage products of carotenoids formed by chemical and enzymatic oxidations. They occur in foods containing carotenoids and thus might be directly absorbed from the diet. However, there is limited information about their intestinal absorption. Read More

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http://dx.doi.org/10.1194/jlr.M093161DOI Listing

Quantitative metabolic profiling of urinary eicosanoids for clinical phenotyping.

J Lipid Res 2019 Mar 6. Epub 2019 Mar 6.

Karolinska Institute, Sweden;

The eicosanoids are a family of lipid mediators of pain and inflammation involved in multiple pathologies, including asthma, hypertension, cancer, atherosclerosis, and neurodegenerative diseases. These signaling mediators act locally, but are rapidly metabolized and transported to the systemic circulation as a mixture of primary and secondary metabolites. Accordingly, urine has become a useful readily accessible biofluid for monitoring the endogenous synthesis of these metabolites. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.D090571
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http://dx.doi.org/10.1194/jlr.D090571DOI Listing
March 2019
19 Reads
4.421 Impact Factor

The PQ-loop protein Any1 segregates Drs2 and Neo1 functions required for viability and plasma membrane phospholipid asymmetry.

J Lipid Res 2019 Mar 1. Epub 2019 Mar 1.

Vanderbilt University, United States.

Membrane asymmetry is a key organizational feature of the plasma membrane. Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that establish membrane asymmetry by translocating phospholipids, such as phosphatidylserine (PS) and phospatidylethanolamine (PE), from the exofacial leaflet to the cytosolic leaflet. Saccharomyces cerevisiae expresses five P4-ATPases: Drs2, Neo1, Dnf1, Dnf2 and Dnf3. Read More

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http://dx.doi.org/10.1194/jlr.M093526DOI Listing

Regulation of lipophagy in NAFLD by cellular metabolism and CD36.

J Lipid Res 2019 Apr 28;60(4):755-757. Epub 2019 Feb 28.

Center for Human Nutrition, Washington University School of Medicine, Saint Louis, MO 63108.

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http://dx.doi.org/10.1194/jlr.C093674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446712PMC

Plasma Lipoprotein-X Quantification on Filipin-Stained Gels: Monitoring Recombinant LCAT Treatment Ex-Vivo.

J Lipid Res 2019 Feb 26. Epub 2019 Feb 26.

National Institutes of Health, United States.

Background: Familial LCAT Deficiency (FLD) patients accumulate LP-X, an abnormal, nephrotoxic lipoprotein enriched in free cholesterol (FC). The low neutral lipid content of LP-X limits the ability to detect it after separation by lipoprotein electrophoresis and staining with Sudan Black or other neutral lipid stains.

Aim: A sensitive and accurate method for quantitating LP-X would be useful to examine the relationship between plasma LP-X and renal disease progression in FLD patients and could also serve as a biomarker for monitoring recombinant human LCAT (rhLCAT) therapy. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.D090233
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http://dx.doi.org/10.1194/jlr.D090233DOI Listing
February 2019
5 Reads

Mammalian lipin phosphatidic acid phosphatases in lipid synthesis and beyond: metabolic and inflammatory disorders.

J Lipid Res 2019 Apr 25;60(4):728-733. Epub 2019 Feb 25.

Department of Human Genetics, David Geffen School of Medicine, and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095.

The regulation of cellular lipid storage and membrane lipid composition plays a critical role in metabolic homeostasis, and dysregulation may contribute to disorders such as obesity, fatty liver, type 2 diabetes, and cardiovascular disease. The mammalian lipin proteins (lipin 1, lipin 2, and lipin 3) are phosphatidic acid phosphatase (PAP) enzymes that modulate levels of cellular triacylglycerols and phospholipids, and also regulate lipid intermediates in cellular signaling pathways. Lipin proteins also have the ability to coactivate/corepress transcription. Read More

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http://dx.doi.org/10.1194/jlr.S091769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446709PMC

LPA Receptor 4 deficiency attenuates experimental atherosclerosis.

J Lipid Res 2019 Feb 22. Epub 2019 Feb 22.

University of Kentucky, United States;

The widely expressed lysophosphatidic acid (LPA) selective receptor 4 (LPAR4) contributes to vascular development in mice and zebrafish. LPAR4 regulates endothelial permeability, lymphocyte migration, and hematopoiesis, which could contribute to atherosclerosis. We investigated the role of LPAR4 in experimental atherosclerosis elicited by adeno-associated virus expressing PCSK9 to lower LDL receptor levels. Read More

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http://dx.doi.org/10.1194/jlr.M091066DOI Listing
February 2019
1 Read

Use of Isotopically Labelled Substrates Reveals Kinetic Differences Between Human and Bacterial Serine Palmitoyltransferase.

J Lipid Res 2019 Feb 21. Epub 2019 Feb 21.

University of Edinburgh, United Kingdom;

Isotope labels are frequently used tools to track metabolites through complex biochemical pathways and to discern the mechanisms of enzyme-catalysed reactions. Isotopically-labelled L-serine is often used to monitor the activity of the first enzyme in sphingolipid biosynthesis, serine palmitoyltransferase (SPT) as well as labelling downstream cellular metabolites. Intrigued by the effect that isotope labels may be having on SPT catalysis, we characterised the impact of different L-serine isotopologues on the catalytic activity of recombinant SPT isozymes from humans and the bacterium Sphingomonas paucimobilis. Read More

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http://dx.doi.org/10.1194/jlr.M089367DOI Listing
February 2019
1 Read

Regulatory T cells suppress excessive lipid accumulation in alcoholic liver disease.

J Lipid Res 2019 Feb 21. Epub 2019 Feb 21.

Huazhong University of Science and Technology, China

Sensitization hepatic immune cells of chronic alcoholic consumption gives rise to inflammatory accumulation which is considered as leading cause to liver damage. T regulatory cells (Tregs) are immunosuppressive cell subset that play an important role in a variety of liver diseases, however, data about pathological involvement of Tregs in liver steatosis of ALD is insufficient. In mouse models of ALD, we found that increased lipid accumulation by chronic alcohol intake as companioned with oxidative stress, inflammatory accumulation as well as Treg decline in the liver. Read More

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http://dx.doi.org/10.1194/jlr.M083568DOI Listing
February 2019
4.421 Impact Factor

Inflammasomes, neutrophil extracellular traps, and cholesterol.

J Lipid Res 2019 Apr 19;60(4):721-727. Epub 2019 Feb 19.

Department of Pediatrics, Section Molecular Genetics, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

Activation of macrophage inflammasomes leads to interleukin (IL)-1β and IL-18 secretion and promotes atherosclerosis and its complications in mice and humans. However, the specific role and underlying mechanisms of the inflammasome in atherogenesis are topics of active research. Several studies in hyperlipidemic mouse models found that the NOD-like receptor protein 3 (NLRP3) inflammasome contributes to atherosclerosis, but recent work suggests that a second hit, such as defective cholesterol efflux or accumulation of oxidized mitochondrial DNA, may be required for significant inflammasome activation. Read More

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http://dx.doi.org/10.1194/jlr.S091280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446695PMC
April 2019
3 Reads

Niacin: an old lipid drug in a new NAD dress.

J Lipid Res 2019 Apr 19;60(4):741-746. Epub 2019 Feb 19.

Laboratory of Integrative Systems Physiology, Interfaculty Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

Niacin, the first antidyslipidemic drug, has been at the center stage of lipid research for many decades before the discovery of statins. However, to date, despite its remarkable effects on lipid profiles, the clinical outcomes of niacin treatment on cardiac events is still debated. In addition to its historically well-defined interactions with central players of lipid metabolism, niacin can be processed by eukaryotic cells to synthesize a crucial cofactor, NAD NAD acts as a cofactor in key cellular processes, including oxidative phosphorylation, glycolysis, and DNA repair. Read More

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http://dx.doi.org/10.1194/jlr.S092007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446705PMC

Rosiglitazone remodels the lipid droplet and britens human visceral and subcutaneous adipocytes ex vivo.

J Lipid Res 2019 Apr 19;60(4):856-868. Epub 2019 Feb 19.

Diabetes, Obesity, and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY.

Treatment with PPARγ agonists in vivo improves human adipocyte metabolism, but the cellular mechanisms and possible depot differences in responsiveness to their effects are poorly understood. To examine the ex vivo metabolic effects of rosiglitazone (Rosi), we cultured explants of human visceral (omental) and abdominal subcutaneous adipose tissues for 7 days. Rosi increased mRNA levels of transcriptional regulators of brite/beige adipocytes (PGC1α, PRDM16), triglyceride synthesis (GPAT3, DGAT1), and lipolysis (ATGL) similarly in adipose tissues from both depots. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.M091173
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http://dx.doi.org/10.1194/jlr.M091173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446708PMC
April 2019
3 Reads

For the sake of science.

J Lipid Res 2019 Apr 13;60(4):719-720. Epub 2019 Feb 13.

Molecular & Cellular Proteomics.

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http://dx.doi.org/10.1194/jlr.E093245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446713PMC

Ces1d deficiency protects against high-sucrose diet-induced hepatic triacylglycerol accumulation.

J Lipid Res 2019 Apr 8;60(4):880-891. Epub 2019 Feb 8.

Group on Molecular and Cell Biology of Lipids University of Alberta, Alberta, Canada

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. Triacylglycerol accumulation in the liver is a hallmark of NAFLD. Metabolic studies have confirmed that increased hepatic de novo lipogenesis (DNL) in humans contributes to fat accumulation in the liver and to NAFLD progression. Read More

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http://dx.doi.org/10.1194/jlr.M092544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446703PMC
April 2019
1 Read

Fructose-induced hypertriglyceridemia in rhesus macaques is attenuated with fish oil or ApoC3 RNA interference.

J Lipid Res 2019 Apr 5;60(4):805-818. Epub 2019 Feb 5.

Department of Molecular Biosciences, School of Veterinary Medicine, California National Primate Research Center, and Department of Nutrition, University of California, Davis, CA

Dyslipidemia and insulin resistance are significant adverse outcomes of consuming high-sugar diets. Conversely, dietary fish oil (FO) reduces plasma lipids. Diet-induced dyslipidemia in a rhesus model better approximates the pathophysiology of human metabolic syndrome (MetS) than rodent models. Read More

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http://dx.doi.org/10.1194/jlr.M089508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446715PMC
April 2019
8 Reads

Simultaneous LC/MS/MS quantification of eight apolipoproteins in normal and hypercholesterolemic mouse plasma.

J Lipid Res 2019 Apr 5;60(4):900-908. Epub 2019 Feb 5.

Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Leipzig, Germany

Apolipoproteins are major structural and functional constituents of lipoprotein particles. As modulators of lipid metabolism, adipose tissue biology, and energy homeostasis, apolipoproteins may serve as biomarkers or potential therapeutic targets for cardiometabolic diseases. Mice are the preferred model to study metabolic disease and CVD, but a comprehensive method to quantify circulating apolipoproteins in mice is lacking. Read More

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http://dx.doi.org/10.1194/jlr.D084301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446716PMC
April 2019
2 Reads

Intramuscular adipocytes: a buried adipose tissue depot deserving more exploration.

J Lipid Res 2019 Apr 4;60(4):753-754. Epub 2019 Feb 4.

Department of Biochemistry, University of Wisconsin, Madison, WI

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http://dx.doi.org/10.1194/jlr.C093047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446714PMC
April 2019
1 Read

Treating cancer with phosphatidylinositol-3-kinase inhibitors: increasing efficacy and overcoming resistance.

J Lipid Res 2019 Apr 4;60(4):747-752. Epub 2019 Feb 4.

Meyer Cancer Center Weill Cornell Medicine, New York, NY 10021

The discovery of the phosphatidylinositol-3-kinase (PI3K) pathway was a major advance in understanding growth factor signaling. The high frequency of PI3K pathway mutations in many cancers has encouraged a new field targeting cancer driver mutations. Although there have been many successes, targeting PI3K itself has proven challenging, in part because of its multiple isoforms with distinct roles. Read More

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http://dx.doi.org/10.1194/jlr.S092130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446698PMC

Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells.

J Lipid Res 2019 Apr 1;60(4):832-843. Epub 2019 Feb 1.

Department of Biochemistry, University of Geneva, 1211-Geneva-4, Switzerland

In specialized cell types, lysosome-related organelles support regulated secretory pathways, whereas in nonspecialized cells, lysosomes can undergo fusion with the plasma membrane in response to a transient rise in cytosolic calcium. Recent evidence also indicates that lysosome secretion can be controlled transcriptionally and promote clearance in lysosome storage diseases. In addition, evidence is also accumulating that low concentrations of cyclodextrins reduce the cholesterol-storage phenotype in cells and animals with the cholesterol storage disease Niemann-Pick type C, via an unknown mechanism. Read More

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http://dx.doi.org/10.1194/jlr.M089979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446697PMC
April 2019
1 Read

Repression of hepatocyte nuclear factor 4 alpha by AP-1 underlies dyslipidemia associated with retinoic acid.

J Lipid Res 2019 Apr 1;60(4):794-804. Epub 2019 Feb 1.

Departments of Pharmacy Practice College of Pharmacy,University of Illinois at Chicago, Chicago, IL

- retinoic acid (atRA) is used to treat certain cancers and dermatologic diseases. A common adverse effect of atRA is hypercholesterolemia; cytochrome P450 (CYP) 7A repression is suggested as a driver. However, the underlying molecular mechanisms remain unclear. Read More

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http://dx.doi.org/10.1194/jlr.M088880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446710PMC

Adipokine FABP4 integrates energy stores and counterregulatory metabolic responses.

J Lipid Res 2019 Apr 30;60(4):734-740. Epub 2019 Jan 30.

Sabri Ülker Center for Metabolic Research Harvard T. H. Chan School of Public Health, Boston, MA

Although counterregulatory hormones and mediators of the fight-or-flight responses are well defined at many levels, how energy stores per se are integrated into this system remains an enigmatic question. Recent years have seen the adipose tissue become a central focus for mediating intracellular signaling and communication through the release of a variety of bioactive lipids and substrates, as well as various adipokines. A critical integration node among these mediators and responses is controlled by FA binding protein 4 (FABP4), also known as adipocyte protein 2 (aP2), which is highly expressed in adipose tissue and functions as a lipid chaperone protein. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.S091793
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http://dx.doi.org/10.1194/jlr.S091793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446704PMC
April 2019
6 Reads
4.421 Impact Factor

LC/MS/MS analyses of open-flow microperfusion samples quantify eicosanoids in a rat model of skin inflammation.

J Lipid Res 2019 Apr 29;60(4):758-766. Epub 2019 Jan 29.

Joanneum Research Forschungsgesellschaft mbH, Institute for Biomedicine and Health Sciences, Graz, Austria

Eicosanoids are lipid-mediator molecules with key roles in inflammatory skin diseases, such as psoriasis. Eicosanoids are released close to the source of inflammation, where they elicit local pleiotropic effects and dysregulations. Monitoring inflammatory mediators directly in skin lesions could provide new insights and therapeutic possibilities. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.M087221
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http://dx.doi.org/10.1194/jlr.M087221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446707PMC
April 2019
10 Reads

Lysophosphatidic acid type 2 receptor agonists in targeted drug development offer broad therapeutic potential.

J Lipid Res 2019 Mar 28;60(3):464-474. Epub 2019 Jan 28.

RxBio Inc. Memphis, TN 38163.

The growth factor-like lipid mediator, lysophosphatidic acid (LPA), is a potent signaling molecule that influences numerous physiologic and pathologic processes. Manipulation of LPA signaling is of growing pharmacotherapeutic interest, especially because LPA resembles compounds with drug-like features. The action of LPA is mediated through activation of multiple types of molecular targets, including six G protein-coupled receptors that are clear targets for drug development. Read More

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http://dx.doi.org/10.1194/jlr.S091744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399510PMC

On the mechanism of angiopoietin-like protein 8 for control of lipoprotein lipase activity.

J Lipid Res 2019 Apr 27;60(4):783-793. Epub 2019 Jan 27.

Department of Medical Biosciences, Umeå University, Umeå, Sweden

Angiopoietin-like (ANGPTL) 8 is a secreted inhibitor of LPL, a key enzyme in plasma triglyceride metabolism. It was previously reported that ANGPTL8 requires another member of the ANGPTL family, ANGPTL3, to act on LPL. ANGPTL3, much like ANGPTL4, is a physiologically relevant regulator of LPL activity, which causes irreversible inactivation of the enzyme. Read More

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http://dx.doi.org/10.1194/jlr.M088807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446706PMC
April 2019
1 Read

It takes a village: channeling fatty acid metabolism and triacylglycerol formation via protein interactomes.

J Lipid Res 2019 Mar 25;60(3):490-497. Epub 2019 Jan 25.

Department of Nutrition, University of North Carolina, Chapel Hill, NC 27599

Diet, hormones, gene transcription, and posttranslational modifications control the hepatic metabolism of FAs; metabolic dysregulation causes chronic diseases, including cardiovascular disease, and warrants exploration into the mechanisms directing FA and triacylglycerol (TAG) synthesis and degradation. Long-chain FA metabolism begins by formation of an acyl-CoA by a member of the acyl-CoA synthetase (ACSL) family. Subsequently, TAG synthesis begins with acyl-CoA esterification to glycerol-3-phosphate by a member of the glycerol-3-phosphate acyltransferase (GPAT) family. Read More

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http://dx.doi.org/10.1194/jlr.S091843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399496PMC

A perilous path: the inborn errors of sphingolipid metabolism.

J Lipid Res 2019 Mar 25;60(3):475-483. Epub 2019 Jan 25.

Genetics of Development and Disease Branch National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892

The sphingolipid (SL) metabolic pathway generates structurally diverse lipids that have roles as membrane constituents and as bioactive signaling molecules. The influence of the SL metabolic pathway in biology is pervasive; it exists in all mammalian cells and has roles in many cellular and physiological pathways. Human genetic diseases have long been recognized to be caused by mutations in the pathway, but until recently these mutational defects were only known to affect lysosomal SL degradation. Read More

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http://www.jlr.org/lookup/doi/10.1194/jlr.S091827
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http://dx.doi.org/10.1194/jlr.S091827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399501PMC
March 2019
8 Reads

Therapeutic FGF19 promotes HDL biogenesis and transhepatic cholesterol efflux to prevent atherosclerosis.

J Lipid Res 2019 Mar 24;60(3):550-565. Epub 2019 Jan 24.

NGM Biopharmaceuticals, Inc., South San Francisco, CA 94080

Fibroblast growth factor (FGF)19, an endocrine hormone produced in the gut, acts in the liver to control bile acid synthesis. NGM282, an engineered FGF19 analog, is currently in clinical development for treating nonalcoholic steatohepatitis. However, the molecular mechanisms that integrate FGF19 with cholesterol metabolic pathways are incompletely understood. Read More

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http://dx.doi.org/10.1194/jlr.M089961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399511PMC
March 2019
2 Reads

Hepatocyte sortilin 1 knockout and treatment with a sortilin 1 inhibitor reduced plasma cholesterol in Western diet-fed mice.

J Lipid Res 2019 Mar 22;60(3):539-549. Epub 2019 Jan 22.

Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160

Sortilin 1 (Sort1) is a member of the Vps10p domain intracellular trafficking receptor family. Genetic variations of the gene are strongly associated with plasma cholesterol levels in humans. Recent studies have linked Sort1 to regulation of cholesterol metabolism in hepatocytes and pro-inflammatory response in macrophages, but the tissue-specific roles of Sort1 in lipid metabolism have not been well defined. Read More

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http://dx.doi.org/10.1194/jlr.M089789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399493PMC
March 2019
2 Reads