185,906 results match your criteria Journal of Interferon & Cytokine Research[Journal]


Cytokines use different intracellular mechanisms to upregulate the membrane expression of CXCR1 in human monocytes.

Mol Immunol 2019 Feb 15;108:23-33. Epub 2019 Feb 15.

Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas- Academia Nacional de Medicina, Buenos Aires, Argentina. Electronic address:

Membrane expression of fractalkine (CXCL1)-receptor (CXCR1) is relevant in monocytes (Mo) because CXCR1-CXCL1 interactions might participate on both, homeostatic and pathologic conditions. We have previously demonstrated that CXCR1 levels are decreased during culture and when Mo are differentiated into dendritic cells, but enhanced when differentiated into macrophages. Regarding soluble factors, lipopolysaccharide (LPS) accelerated the loss of CXCR1, while interleukin (IL)-10 and Interferon-gamma (IFN-γ) prevented it. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.003DOI Listing
February 2019

Receptor interacting protein kinases-dependent necroptosis as a new, potent mechanism for elimination of the endothelial cells during luteolysis in cow.

Theriogenology 2019 Feb 4;128:193-200. Epub 2019 Feb 4.

Division of Biology of Reprodcution, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. Electronic address:

Necroptosis is an alternative form of programmed cell death regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent. In the present study, to clarify if necroptosis in luteal endothelial cells (LECs) participates and contributes for bovine luteolysis, we investigated RIPK1 and RIPK3 localization in luteal tissue and their expression in cultured LECs after treatment with selected immune factors - mediators of luteolytic action of prostaglandin F2α (PGF). In addition, effects of tumor necrosis factor α (TNF; 2. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2019.01.035DOI Listing
February 2019

Permeability analyses and three dimensional imaging of interferon gamma-induced barrier disintegration in intestinal organoids.

Stem Cell Res 2019 Feb 7;35:101383. Epub 2019 Feb 7.

Division of Molecular and Experimental Surgery, Department of Surgery, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, Translational Research Center, 91054 Erlangen, Germany; Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91052 Erlangen, Germany.

The aberrant regulation of the epithelial barrier integrity is involved in many diseases of the digestive tract, including inflammatory bowel diseases and colorectal cancer. Intestinal epithelial cell organoid cultures provide new perspectives for analyses of the intestinal barrier in vitro. However, established methods of barrier function analyses from two dimensional cultures have to be adjusted to the analysis of three dimensional organoid structures. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101383DOI Listing
February 2019

Type I interferon responses of common carp strains with different levels of resistance to koi herpesvirus disease during infection with CyHV-3 or SVCV.

Fish Shellfish Immunol 2019 Feb 15. Epub 2019 Feb 15.

Fish Disease Research Unit, Institute for Parasitology, University of Veterinary Medicine, Hannover, Germany.

Carp from breeding strains with different genetic background present diverse levels of resistance to viral pathogens. Carp strains of Asian origin, currently being treated as Cyprinus rubrofuscus L., especially Amur wild carp (AS), were proven to be more resistant to koi herpesvirus disease (KHVD; caused by cyprinid herpesvirus 3, CyHV-3) than strains originating from Europe and belonging to Cyprinus carpio L. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.022DOI Listing
February 2019

House dust mite exposure attenuates influenza A infection in a mouse model of pulmonary allergic inflammation.

Microb Pathog 2019 Feb 15. Epub 2019 Feb 15.

Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA. Electronic address:

Environmental allergens elicit complex immune responses in the lungs that can promote the development of asthma or exacerbate preexisting asthma in susceptible individuals. House dust mites are one of the most common indoor allergens and are a significant driver of allergic disease. Respiratory infections are known factors in acute exacerbations of asthma but the impact of allergen on the pathogen is not well understood. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.02.019DOI Listing
February 2019

Microencapsulated Immunoassays for Detection of Cytokines in Human Blood.

ACS Sens 2019 Feb 18. Epub 2019 Feb 18.

Department of Physiology and Biomedical Engineering , Mayo Clinic , Rochester Campus, Rochester , Minnesota 55905 , United States.

Cytokines are produced by leukocytes in blood and may be used as indicators of malignancies or infections. The objective of this study was to develop a strategy for immunosensing cytokines in whole, unprocessed human blood. Microfluidic droplet generation was employed to fabricate ∼400 μm diameter microcapsules with a hydrogel shell and an aqueous core containing sensing microbeads. Read More

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http://dx.doi.org/10.1021/acssensors.8b01033DOI Listing
February 2019

High level extracellular production of recombinant human interferon alpha 2b in glycoengineered Pichia pastoris: culture medium optimization, high cell density cultivation and biological characterization.

J Appl Microbiol 2019 Feb 18. Epub 2019 Feb 18.

BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.

Aims: The present study was aimed at design of experiments (DoE) and artificial intelligence based culture medium optimization for high level extracellular production of a novel recombinant human interferon alpha 2b (huIFNα2b) in glycoengineered Pichia pastoris and its characterization.

Methods And Results: Artificial neural network- Genetic algorithm (ANN-GA) model exhibited improved huIFNα2b production and better predictability compared to RSM. The optimized medium exhibited 5-fold increase in huIFNα2b titer compared to the complex medium. Read More

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http://dx.doi.org/10.1111/jam.14227DOI Listing
February 2019
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Methionine Attenuates Lipopolysaccharide-Induced Inflammatory Responses via DNA Methylation in Macrophages.

ACS Omega 2019 Jan 30;4(1):2331-2336. Epub 2019 Jan 30.

State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.

Methionine (Met) is an essential and multifunctional nutrient in vertebrate diets. It is a precursor of -adenosylmethionine (SAM), the methyl donor for DNA methylation, which has an important role in the inflammatory responses. However, whether Met exerts anti-inflammatory effects by altering DNA methylation in macrophages is unclear. Read More

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http://dx.doi.org/10.1021/acsomega.8b03571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374979PMC
January 2019

Gene Expression Studies and Targeted Metabolomics Reveal Disturbed Serine, Methionine, and Tyrosine Metabolism in Early Hypertensive Nephrosclerosis.

Kidney Int Rep 2019 Feb 17;4(2):321-333. Epub 2018 Oct 17.

Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.

Introduction: Hypertensive nephrosclerosis is among the leading causes of end-stage renal disease, but its pathophysiology is poorly understood. We wanted to explore early metabolic changes using gene expression and targeted metabolomics analysis.

Methods: We analyzed gene expression in kidneys biopsied from 20 patients with nephrosclerosis and 31 healthy controls with an Affymetrix array. Read More

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http://dx.doi.org/10.1016/j.ekir.2018.10.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365407PMC
February 2019

NDR2 promotes the antiviral immune response via facilitating TRIM25-mediated RIG-I activation in macrophages.

Sci Adv 2019 Feb 6;5(2):eaav0163. Epub 2019 Feb 6.

Institute of Immunology, School of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang, China.

Retinoic acid-inducible gene I (RIG-I), a pivotal cytosolic sensor, recognizes viral RNAs to initiate antiviral innate immunity. However, posttranslational regulation of RIG-I signaling is not well understood. We report here that nuclear Dbf2-related kinase 2 (NDR2) functions as a crucial positive regulator of the RIG-I-mediated antiviral immune response. Read More

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http://dx.doi.org/10.1126/sciadv.aav0163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365120PMC
February 2019

Measles Virus-Based Treatments Trigger a Pro-inflammatory Cascade and a Distinctive Immunopeptidome in Glioblastoma.

Mol Ther Oncolytics 2019 Mar 31;12:147-161. Epub 2018 Dec 31.

Interdisciplinary Division of Neuro-Oncology, Hertie Institute for Clinical Brain Research, Departments of Neurology and Neurosurgery, University Hospital Tübingen, Eberhard Karls University Tübingen, Tübingen 72076, Germany.

Glioblastoma is an aggressive primary brain tumor with bad prognosis. On the other hand, oncolytic measles virus (MeV) therapy is an experimental glioma treatment strategy with clinical safety and first evidence of anti-tumoral efficacy. Therefore, we investigated the combination of MeV with conventional therapies by cytotoxic survival assays in long-term glioma cell lines LN229, LNZ308, and glioma stem-like GS8 cells, as well as the basal viral infectivity in primary glioblastoma cultures T81/16, T1094/17, and T708/16. Read More

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http://dx.doi.org/10.1016/j.omto.2018.12.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365369PMC

The Immunology of Macrophage Activation Syndrome.

Front Immunol 2019 1;10:119. Epub 2019 Feb 1.

Pediatric Rheumatology, University of Alabama Birmingham, Birmingham, AL, United States.

Synonymous with secondary hemophagocytic lymphohistiocytosis, macrophage activation syndrome (MAS) is a term used by rheumatologists to describe a potentially life-threatening complication of systemic inflammatory disorders, most commonly systemic juvenile idiopathic arthritis (sJIA) and systemic lupus erythematosus (SLE). Clinical and laboratory features of MAS include sustained fever, hyperferritinemia, pancytopenia, fibrinolytic coagulopathy, and liver dysfunction. Soluble interleukin-2 receptor alpha chain (sCD25) and sCD163 may be elevated, and histopathology often reveals characteristic increased hemophagocytic activity in the bone marrow (and other tissues), with positive CD163 (histiocyte) staining. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367262PMC
February 2019

Programmed death ligand-1, tumor infiltrating lymphocytes and HLA expression in Chinese extrahepatic cholangiocarcinoma patients: Possible immunotherapy implications.

Biosci Trends 2019 Feb 15. Epub 2019 Feb 15.

School of Clinical Medicine, Tsinghua University.

Immunotherapy might be an effective treatment in extrahepatic cholangiocarcinoma (eCCA), a tumor with extremely limited therapeutic options. Our study is to characterize the programmed death ligand-1 (PD-L1) protein expression and cancer microenvironment profiles in surgically resected eCCA samples. PD-L1 positivity was observed on tumor cells (32. Read More

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http://dx.doi.org/10.5582/bst.2019.01003DOI Listing
February 2019

VaccHemInf project: protocol for a prospective cohort study of efficacy, safety and characterisation of immune functional response to vaccinations in haematopoietic stem cell transplant recipients.

BMJ Open 2019 Feb 15;9(2):e026093. Epub 2019 Feb 15.

Département des Maladies Infectieuses et Tropicales, Hospices Civils de Lyon, Lyon, France.

Introduction: Immune reconstitution after haematopoietic stem cell transplantation (HSCT) is a complex and dynamic process, varying from a state of nearly complete immunosuppression to an expected full immune recovery. Specific vaccination guidelines recommend reimmunisation after HSCT but data regarding vaccine efficacy in this unique population are scarce. New immune functional assays could enable prediction of vaccine response in the setting of HSCT. Read More

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http://dx.doi.org/10.1136/bmjopen-2018-026093DOI Listing
February 2019

The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Department of Orthopedics, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, PR China.

Ischemia reperfusion injury (IRI), a complex phenomenon often encountered in surgery, can lead to local and distant tissue destruction and sometimes even death. microRNA-203 (miR-203) has been reported to negatively regulate ischemia induced microglia activation with a feedback to myeloid differentiation primary-response gene 88 (MYD88). Accordingly, our study is to verify the effect of miR-203 and MYD88 on mice in IRI after total knee arthroplasty (TKA). Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.030DOI Listing
February 2019

STING-associated lung disease in mice relies on T cells but not type I interferon.

J Allergy Clin Immunol 2019 Feb 14. Epub 2019 Feb 14.

Pathology and Immunology, Saint Louis, MO, USA 63110; Molecular Microbiology, Saint Louis, MO, USA 63110; Washington University School of Medicine, Saint Louis, MO, USA 63110. Electronic address:

Background: Monogenic interferonopathies are thought to be mediated by type I interferon (IFN). For example, a gain-of-function mutation in STING (STING N153S) up-regulates type I IFN-stimulated genes (ISGs) and causes perivascular inflammatory lung disease in mice. The equivalent mutation in humans also causes lung disease, which is thought to require signaling through the cGAS-STING pathway and subsequent activation of IFN regulatory factors (IRF) 3/7, type I IFN, and ISGs. Read More

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http://dx.doi.org/10.1016/j.jaci.2019.01.044DOI Listing
February 2019

Enhanced type I interferon gene signature in primary antiphospholipid syndrome: Association with earlier disease onset and preeclampsia.

Autoimmun Rev 2019 Feb 14. Epub 2019 Feb 14.

Rheumatology Division, Faculdade de Medicina da Universidade de São Paulo (USP), Brazil.

Objective: Recently, two studies demonstrated that a relevant percentage of primary antiphospholipid syndrome (PAPS) patients had an upregulation of interferon (IFN) genes. However, 20%-28% of these patients had anti-dsDNA, a highly specific systemic lupus erythematosus (SLE) autoantibody. This study aimed to determine the prevalence of the type I IFN signature in the peripheral blood mononuclear cells of PAPS patients without specific SLE autoantibodies and search for its clinical associations. Read More

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http://dx.doi.org/10.1016/j.autrev.2018.11.004DOI Listing
February 2019

Current Treatment and Management of Infantile Hemangiomas.

Surv Ophthalmol 2019 Feb 14. Epub 2019 Feb 14.

Department of Ophthalmology, University of Washington, WA, USA; Department of Oculoplastic and Reconstructive Surgery, University of Washington, WA, USA. Electronic address:

Infantile hemangiomas (IHs), otherwise known as infantile capillary hemangiomas, strawberry hemangiomas, or strawberry nevi, are non-malignant vascular tumors that commonly affect children. The natural disease course typically involves growth for up to a year followed by regression without treatment over a period of years, with no cosmetic or functional sequelae. Less commonly, however, IHs can become a threat to vision or even life depending on location and size of the lesion. Read More

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http://dx.doi.org/10.1016/j.survophthal.2019.02.005DOI Listing
February 2019

Resolving Cell Fate Decisions during Somatic Cell Reprogramming by Single-Cell RNA-Seq.

Mol Cell 2019 Feb 13. Epub 2019 Feb 13.

CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou 510530, China; Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou 511436, China; University of Chinese Academy of Sciences, Beijing 100049, China; Guangzhou Regenerative Medicine and Health GuangDong Laboratory (GRMH-GDL), Guangzhou 510005, China. Electronic address:

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs), which is a highly heterogeneous process. Here we report the cell fate continuum during somatic cell reprogramming at single-cell resolution. We first develop SOT to analyze cell fate continuum from Oct4/Sox2/Klf4- or OSK-mediated reprogramming and show that cells bifurcate into two categories, reprogramming potential (RP) or non-reprogramming (NR). Read More

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http://dx.doi.org/10.1016/j.molcel.2019.01.042DOI Listing
February 2019

Role of eosinophils in a murine model of inflammatory bowel disease.

Biochem Biophys Res Commun 2019 Feb 13. Epub 2019 Feb 13.

Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan.

Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD), which is triggered spontaneously by unknown mechanisms and manifests as chronic and relapsing inflammatory conditions in the colon. Eosinophil infiltration is often observed in the colonic tissue of ulcerative colitis patients. However, the role of eosinophils in the disease has not been well defined. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.02.056DOI Listing
February 2019

The role of type I interferons in CD4 T cell differentiation.

Immunol Lett 2019 Feb 13. Epub 2019 Feb 13.

Division of Immunology, Transplantation and Infectious Diseases and Experimental Imaging Center, IRCCS San Raffaele Scientific Institute and Vita-Salute San Raffaele University, Milan, 20132, Italy. Electronic address:

Type I interferons (IFNs) released upon viral infections play different and opposing roles in disease outcome. This pleiotropic effect is mainly influenced by the cellular sources, timing and target cells for these molecules. The effect of type I IFN signaling on the activation and differentiation of antiviral CD4 T cells remains ill defined, with studies reporting either a beneficial or a detrimental role, depending on the context of infection. Read More

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http://dx.doi.org/10.1016/j.imlet.2019.01.013DOI Listing
February 2019

New treatment option for delta virus: is a cure in sight?

Authors:
Cihan Yurdaydin

J Viral Hepat 2019 Feb 16. Epub 2019 Feb 16.

Department of Gastroenterology, Ankara University Medical School, Cebeci Tıp Fakültesi Hastanesi, Dikimevi, 06100, Ankara, Turkey.

Current treatment of chronic hepatitis D viral infection with interferons is poorly tolerated and effective only in a minority of patients. Despite Delta virus causing the most severe form of chronic viral hepatitis no other treatments are available. After many years of inactivity there is now hope for new treatment approaches for Delta virus and some are likely to enter clinical practice in the near future. Read More

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http://doi.wiley.com/10.1111/jvh.13081
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http://dx.doi.org/10.1111/jvh.13081DOI Listing
February 2019
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The effect of L-theanine supplementation on the immune system of athletes exposed to strenuous physical exercise.

J Int Soc Sports Nutr 2019 Feb 15;16(1). Epub 2019 Feb 15.

Department of Morphological and Health Sciences, Faculty of Physical Culture in Gorzów Wielkopolski, 13 Estkowskiego Str, 66-400, Gorzów Wielkopolski, Poland.

Background: The aim of this study was to analyze the response of selected components of the immune system in rowers to maximal physical exercise, and to verify if this response could be modulated by supplementation with L-theanine.

Method: The double-blind study included 20 members of the Polish Rowing Team. The subjects were randomly assigned to the supplemented group (n = 10), receiving 150 mg of L-theanine extract for 6 weeks, or to the placebo group (n = 10). Read More

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http://dx.doi.org/10.1186/s12970-019-0274-yDOI Listing
February 2019

Innate immunity and interferons in the pathogenesis of Sjögren's syndrome.

Rheumatology (Oxford) 2019 Feb 15. Epub 2019 Feb 15.

Department of Medicine, Rheumatology Unit, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.

Primary SS (pSS) is a rheumatic disease characterized by an immune-mediated exocrinopathy, resulting in severe dryness of eyes and mouth. Systemic symptoms include fatigue and joint pain and a subset of patients develop more severe disease with multi-organ involvement. Accumulating evidence points to involvement of innate immunity and aberrant activity of the type I IFN system in both the initiation and propagation of this disease. Read More

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http://dx.doi.org/10.1093/rheumatology/key360DOI Listing
February 2019

Characteristics of circulating natural killer cells and their interferon-γ production in active adult-onset Still's disease.

J Rheumatol 2019 Feb 15. Epub 2019 Feb 15.

Department of Medicine (Neurology and Rheumatology), Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan; Institute for Biomedical Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto 390-8621, Japan. Corresponding author: Yasuhiro Shimojima Department of Medicine (Neurology and Rheumatology), Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto 390-8621, Japan. E-mail:

Objective: To investigate the characteristics of circulating NK cells and their IFN-γ-producing ability in adult-onset Still's disease (AOSD).

Methods: Peripheral blood mononuclear cells were obtained from 22 patients in the acute phase of AOSD (acute AOSD); 7 of the 22 patients after treatment (remission AOSD), and 11 healthy controls (HC). NK cells and their IFN-γ expression levels were analyzed by flow cytometry. Read More

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http://dx.doi.org/10.3899/jrheum.181192DOI Listing
February 2019

The search for a PKR code - Differential regulation of Protein Kinase R activity by diverse RNA and protein regulators.

RNA 2019 Feb 15. Epub 2019 Feb 15.

National Institute of Diabetes and Digestive and Kidney Diseases

The interferon-inducible protein kinase R (PKR) is a key component of host innate immunity that restricts viral replication and propagation. As one of the four eIF2alpha kinases that sense diverse stresses and direct the integrated stress response (ISR) crucial for cell survival and proliferation, PKR's versatile roles extend well beyond antiviral defense. Targeted by numerous host and viral regulators made of RNA and proteins, PKR is subject to multiple layers of endogenous control and external manipulation, driving its rapid evolution. Read More

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http://dx.doi.org/10.1261/rna.070169.118DOI Listing
February 2019

Progression of mycosis fungoides occurs through divergence of tumor immunophenotype by differential expression of HLA-DR.

Blood Adv 2019 Feb;3(4):519-530

Instititute of Immunology and Immunotherapy, University of Birmingham, Birmingham, United Kingdom; and.

Immunotherapy is a valuable treatment for many cancer patients, and there is considerable interest in understanding the mechanisms of immune evasion to guide appropriate management. Mycosis fungoides (MF) is a malignant disorder of skin-homing CD4 T cells, and it exhibits a highly variable clinical course during which the tumor-specific immune response may be an important determinant. An unusual feature of MF is that tumor-infiltrating lymphocytes (TILs) must attempt to control a malignant cell from within their own lineage. Read More

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http://dx.doi.org/10.1182/bloodadvances.2018025114DOI Listing
February 2019

ATR inhibition potentiates the radiation induced inflammatory tumour microenvironment.

Clin Cancer Res 2019 Feb 15. Epub 2019 Feb 15.

Oncology, Institute of Cancer Research.

Purpose: ATR inhibitors (ATRi) are in early phase clinical trials and have been shown to sensitise to chemotherapy and radiotherapy preclinically. No data have been published about the effect of these drugs on the tumor microenvironment.

Experimental Design: We used an immunocompetent mouse model of HPV-driven malignancies to investigate the ATR inhibitor AZD6738 in combination with fractionated radiation (RT). Read More

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http://dx.doi.org/10.1158/1078-0432.CCR-18-1821DOI Listing
February 2019

The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.

Immunity 2019 Feb 6. Epub 2019 Feb 6.

Michael Smith Laboratories, University of British Columbia (UBC), 2185 East Mall, Vancouver, BC, V6T 1Z4, Canada; The Vancouver Prostate Centre, 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada; Department of Microbiology and Immunology, UBC, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada; Centre for Blood Research, UBC, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada; Department of Zoology, UBC, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada; Department of Medical Genetics, UBC, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada; Djavad Mowafaghian Centre for Brain Health, 2215 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada. Electronic address:

Sepsis is a bi-phasic inflammatory disease that threatens approximately 30 million lives and claims over 14 million annually, yet little is known regarding the molecular switches and pathways that regulate this disease. Here, we have described ABCF1, an ATP-Binding Cassette (ABC) family member protein, which possesses an E2 ubiquitin enzyme activity, through which it controls the Lipopolysaccharide (LPS)- Toll-like Receptor-4 (TLR4) mediated gram-negative insult by targeting key proteins for K63-polyubiquitination. Ubiquitination by ABCF1 shifts the inflammatory profile from an early phase MyD88-dependent to a late phase TRIF-dependent signaling pathway, thereby regulating TLR4 endocytosis and modulating macrophage polarization from M1 to M2 phase. Read More

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http://dx.doi.org/10.1016/j.immuni.2019.01.014DOI Listing
February 2019

THO Complex Subunit 7 Homolog Negatively Regulates Cellular Antiviral Response against RNA Viruses by Targeting TBK1.

Viruses 2019 Feb 15;11(2). Epub 2019 Feb 15.

Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang 330022, Jiangxi, China.

RNA virus invasion induces a cytosolic RIG-I-like receptor (RLR) signaling pathway by promoting assembly of the Mitochondrial antiviral-signaling protein (MAVS) signalosome and triggers the rapid production of type I interferons (IFNs) and proinflammatory cytokines. During this process, the pivotal kinase TANK binding kinase 1 (TBK1) is recruited to the MAVS signalosome to transduce a robust innate antiviral immune response by phosphorylating transcription factors interferon regulatory factor 3 (IRF3) and nuclear factor (NF)-κB and promoting their nuclear translocation. However, the molecular mechanisms underlying the negative regulation of TBK1 are largely unknown. Read More

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http://dx.doi.org/10.3390/v11020158DOI Listing
February 2019

Dynamic Expression of Interferon Lambda Regulated Genes in Primary Fibroblasts and Immune Organs of the Chicken.

Genes (Basel) 2019 Feb 14;10(2). Epub 2019 Feb 14.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Interferons (IFNs) are pleiotropic cytokines that establish a first line of defense against viral infections in vertebrates. Several types of IFN have been identified; however, limited information is available in poultry, especially using live animal experimental models. IFN-lambda (IFN-λ) has recently been shown to exert a significant antiviral impact against viral pathogens in mammals. Read More

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http://dx.doi.org/10.3390/genes10020145DOI Listing
February 2019

Evaluation of brain volume alterations in HCV-infected patients after interferon-free therapy: A pilot study.

J Neurol Sci 2019 Feb 7;399:36-43. Epub 2019 Feb 7.

Department of General Radiology, Interventional Radiology and Neuroradiology, Radiology, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland.

The study was performed to evaluate cerebral volume changes in HCV-infected subjects before and after interferon-free therapy with direct-acting antiviral agents (DAA). We aimed also to estimate the impact of successful DAA therapy on the neuropsychological state of patients. Eleven HCV genotype 1 (GT1) patients treated with ombitasvir/paritaprevir (boosted with ritonavir) and dasabuvir, with or without ribavirin underwent brain magnetic resonance (MR) before and 24 weeks after completion of therapy. Read More

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http://dx.doi.org/10.1016/j.jns.2019.02.002DOI Listing
February 2019

Increasing of the interferon-γ gene expression during polyomavirus BK infection in kidney transplant patients.

Microb Pathog 2019 Feb 12. Epub 2019 Feb 12.

Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

Polyomavirus BK infection is a common complication and a major cause of morbidity after kidney transplantation. Surveillance of kidney transplant recipients was threatened by reactivation of polyomavirus BK infection can lead to polyomavirus BK-associated nephropathy (PVN). Antiviral immunoregulatory markers like Gamma interferon (IFN-γ) might also affect the polyomavirus BK pathogenesis for its role in antiviral host defense, graft rejection, and regulative of the adaptive immune responses. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.02.015DOI Listing
February 2019

Evaluation of a human in vitro skin test for predicting drug hypersensitivity reactions.

Toxicol Appl Pharmacol 2019 Feb 12. Epub 2019 Feb 12.

Alcyomics Ltd, Bulman House, Regent Centre, Gosforth, Newcastle-upon-Tyne NE3 3LS, United Kingdom; Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle-upon-Tyne NE2 4HH, United Kingdom. Electronic address:

The occurrence of drug hypersensitivity reactions (DHRs) following administration of low molecular weight (LMW) drugs is an important health concern. However, in vivo animal models which could be used as tools for the prediction of DHRs are lacking. As a result, research has focused on development of in vitro tools for predicting DHRs. Read More

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http://dx.doi.org/10.1016/j.taap.2019.02.005DOI Listing
February 2019

Cyclical adaptation of measles virus quasispecies to epithelial and lymphocytic cells: To V, or not to V.

PLoS Pathog 2019 Feb 15;15(2):e1007605. Epub 2019 Feb 15.

Department of Molecular Medicine, Mayo Clinic, Rochester, MN, United States of America.

Measles virus (MeV) is dual-tropic: it replicates first in lymphatic tissues and then in epithelial cells. This switch in tropism raises the question of whether, and how, intra-host evolution occurs. Towards addressing this question, we adapted MeV either to lymphocytic (Granta-519) or epithelial (H358) cells. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007605DOI Listing
February 2019

Effect of cooled perches on physiological parameters of caged White Leghorn hens exposed to cyclic heat.

Poult Sci 2019 Feb 15. Epub 2019 Feb 15.

USDA-Agricultural Research Service, Livestock Behavior Research Unit, West Lafayette, IN 47907.

We assessed the effects of water-chilled perches as a cooling device on the physiological parameters of caged laying hens exposed to 2 cyclic heating episodes. White Leghorns, 17 wk of age, were randomly assigned to 36 cages of 6 banks placed in the same room. Each bank was randomly assigned to 1 of 3 treatments: cooled perch (CP), air perch (AP), and no perch (CTRL) resulting in 2 replicate banks and 12 cages per treatment. Read More

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http://dx.doi.org/10.3382/ps/pez012DOI Listing
February 2019

Proteomic Changes in Oral Keratinocytes Chronically Exposed to Shisha (Water Pipe).

OMICS 2019 Feb;23(2):86-97

1 Department of Medical Biotechnologies, School of Dental Medicine, University of Siena, Siena, Italy.

Shisha (water pipe) smoking is falsely believed to be a hazard-free habit and has become a major public health concern. Studies have reported shisha smoking to be associated with oral lesions, as well as carcinomas of the lung, esophagus, bladder, and pancreas. A deeper understanding of the underlying molecular mechanisms would contribute to identification of biomarkers for targeted public health screening, therapeutic innovation, and better prognosis of associated diseases. Read More

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http://dx.doi.org/10.1089/omi.2018.0173DOI Listing
February 2019

Molecular mechanism of interactions between Chrysin and I-kappa-B kinase epsilon (IKKe)/Tank Binding Kinase-1(TBK1): Cell based assay and insilico molecular docking studies.

J Biomol Struct Dyn 2019 Feb 15:1-9. Epub 2019 Feb 15.

a Faculty of Pharmacy , Integral University , Lucknow , Uttar Pradesh , India .

Chrysin, a bioactive flavonoid, was investigated for its potential to inhibit the activity of I-kappa-B kinase epsilon (IKKe) / Tank Binding Kinase-1(TBK1) an enzyme responsible for production of pro inflammatory cytokine and suppression of energy expenditure genes, finally causing insulin resistance and obesity. Expressions of majority of polyinosinic-polycytidylic acid (poly IC) mediated genes are mediated through Toll/interleukin-1 receptor domainߝcontaining adapter-inducing interferon (TRIF) dependent signalling pathway. To check the therapeutic potential of chrysin its effect was examined on Toll/interleukin-1 receptor domainߝcontaining adapter-inducing interferon (TRIF) dependent pathway. Read More

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http://dx.doi.org/10.1080/07391102.2019.1581086DOI Listing
February 2019

Fractionated ionizing radiation facilitates interferon-γ signaling and anticancer activity in lung adenocarcinoma cells.

J Cell Physiol 2019 Feb 14. Epub 2019 Feb 14.

Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Fractionated ionizing radiation (FIR) is a radiotherapy regimen that is regularly performed as part of lung cancer treatment. In contrast to the growth inhibition caused by DNA damage, immunomodulation in post-irradiated cancer cells is not well documented. Interferon (IFN)-γ confers anticancer activity by triggering both growth inhibition and cytotoxicity. Read More

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http://dx.doi.org/10.1002/jcp.28258DOI Listing
February 2019

Recent Innovations in Diagnosis and Treatment of Pediatric Tuberculosis.

Curr Infect Dis Rep 2019 Feb 15;21(1). Epub 2019 Feb 15.

Medicine-Pediatric Residency Training Program, University of Colorado School of Medicine, Aurora, CO, USA.

Purpose Of Review: Tuberculosis is leading cause of global morbidity and mortality and a significant proportion of the burden of disease occurs in children. In the past 5 years, a number of innovations have improved the diagnosis and treatment for children with both latent tuberculosis infection and active disease.

Recent Findings: This review discusses three key areas of innovation. Read More

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http://link.springer.com/10.1007/s11908-019-0662-0
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http://dx.doi.org/10.1007/s11908-019-0662-0DOI Listing
February 2019
3 Reads

Interferon-γ receptor-1 gene promoter polymorphisms and susceptibility for brucellosis in Makkah region.

Afr Health Sci 2018 Dec;18(4):1157-1165

Department of Medical Microbiology, Faculty of Applied Medical Sciences, Taif University, Turabah, 21995, KSA.

Background: Genetic polymorphisms that affect the production levels of certain cytokines and/or their receptors may determine the risk, severity or protection in some infectious diseases like brucellosis.

Objectives: The aim of this study was to investigate the association of certain known Interferon-γ Receptor-1 (IFN-γ R1) gene promoter polymorphisms and the susceptibility to infection with Brucellosis in Saudi population.

Methods: A cases-control association study was conducted in 69 individuals with human brucellosis and 94 healthy individuals. Read More

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http://dx.doi.org/10.4314/ahs.v18i4.36DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354847PMC
December 2018

Phagosome Escape of Rough Strains in Murine Macrophage via Phagosomal Rupture Can Lead to Type I Interferon Production and Their Cell-To-Cell Spread.

Front Immunol 2019 31;10:125. Epub 2019 Jan 31.

Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, College of Medicine, Seoul National University, Seoul, South Korea.

complex (MAB) is a rapidly growing mycobacterium(RGM) whose clinical significance as an emerging human pathogen has been increasing worldwide. It has two types of colony morphology, a smooth (S) type, producing high glycopeptidolipid (GPL) content, and a rough (R) type, which produces low levels of GPLs and is associated with increased virulence. However, the mechanism responsible for their difference in virulence is poorly known. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365470PMC
January 2019

Macrophage Activation-Like Syndrome: A Distinct Entity Leading to Early Death in Sepsis.

Front Immunol 2019 31;10:55. Epub 2019 Jan 31.

Fourth Department of Internal Medicine, Medical School, National and Kapodistrian University of Athens, Athens, Greece.

Hemophagocytic lymphohistocytosis (HLH) is characterized by fulminant cytokine storm leading to multiple organ dysfunction and high mortality. HLH is classified into familial (fHLH) and into secondary (sHLH). fHLH is rare and it is due to mutations of genes encoding for perforin or excretory granules of natural killer (NK) cells of CD8-lymphocytes. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365431PMC
January 2019

Results of Sofosbuvir Plus Ribavirin in Patients With Hepatitis C Related Decompensated Cirrhosis.

J Clin Exp Hepatol 2019 Jan-Feb;9(1):4-12. Epub 2018 Mar 6.

Institute of Liver, Gastroenterology, & Panceatico-Biliary Sciences, Sir Ganga Ram Hospital, New Delhi, India.

Background: Sofosbuvir (SOF), a direct acting antiviral, has revolutionized the treatment of chronic Hepatitis C Virus (HCV) infection. However, data is scarce about efficacy of SOF plus Ribavarin (RBV) in Indian patients with decompensated cirrhosis. We evaluated the efficacy of SOF plus RBV in decompensated cirrhosis, and compared the outcome with compensated cirrhosis and non-cirrhotics. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09736883183004
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http://dx.doi.org/10.1016/j.jceh.2018.02.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363947PMC
March 2018
2 Reads

Interferon-stimulated gene 15 modulates cell migration by interacting with Rac1 and contributes to lymph node metastasis of oral squamous cell carcinoma cells.

Oncogene 2019 Feb 14. Epub 2019 Feb 14.

National Institute of Cancer Research, National Health Research Institutes, Miaoli, Taiwan.

In an effort to understand the underlying mechanisms of lymph node metastasis in oral squamous cell carcinoma (OSCC), through in vivo selection, LN1-1 cells were previously established from OEC-M1 cells and showed enhanced lymphangiogenesis and lymphatic metastasis capabilities. In the current study, we use a stable isotope labeling with amino acids in cell culture (SILAC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic platform to compare LN1-1 to OEC-M1 cells. Interferon-stimulated gene 15 (ISG15) was found highly expressed in LN1-1 cells. Read More

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http://dx.doi.org/10.1038/s41388-019-0731-8DOI Listing
February 2019

The long non-coding RNA Meg3 is dispensable for hematopoietic stem cells.

Sci Rep 2019 Feb 14;9(1):2110. Epub 2019 Feb 14.

Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ), 69120, Heidelberg, Germany.

The long non-coding RNA (lncRNA) Maternally Expressed Gene 3 (Meg3) is encoded within the imprinted Dlk1-Meg3 gene locus and is only maternally expressed. Meg3 has been shown to play an important role in the regulation of cellular proliferation and functions as a tumor suppressor in numerous tissues. Meg3 is highly expressed in mouse adult hematopoietic stem cells (HSCs) and strongly down-regulated in early progenitors. Read More

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http://www.nature.com/articles/s41598-019-38605-8
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http://dx.doi.org/10.1038/s41598-019-38605-8DOI Listing
February 2019
1 Read

Analysis of whole genome-transcriptomic organization in brain to identify genes associated with alcoholism.

Transl Psychiatry 2019 Feb 14;9(1):89. Epub 2019 Feb 14.

Department of Neuroscience, Icahn School of Medicine at Mt. Sinai, 1425 Madison Ave, New York, NY, USA.

Alcohol exposure triggers changes in gene expression and biological pathways in human brain. We explored alterations in gene expression in the Pre-Frontal Cortex (PFC) of 65 alcoholics and 73 controls of European descent, and identified 129 genes that showed altered expression (FDR < 0.05) in subjects with alcohol dependence. Read More

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http://dx.doi.org/10.1038/s41398-019-0384-yDOI Listing
February 2019

Parallel adaptation of rabbit populations to myxoma virus.

Science 2019 Feb 14. Epub 2019 Feb 14.

Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.

In the 1950s the myxoma virus was released into European rabbit populations in Australia and Europe, decimating populations and resulting in the rapid evolution of resistance. We investigated the genetic basis of resistance by comparing the exomes of rabbits collected before and after the pandemic. We found a strong pattern of parallel evolution, with selection on standing genetic variation favoring the same alleles in Australia, France and the United Kingdom. Read More

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http://www.sciencemag.org/lookup/doi/10.1126/science.aau7285
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http://dx.doi.org/10.1126/science.aau7285DOI Listing
February 2019
2 Reads

Clinical outcomes in patients with chronic hepatitis C after direct-acting antiviral treatment: a prospective cohort study.

Lancet 2019 Feb 11. Epub 2019 Feb 11.

AP-HP, Hôpital Cochin, Unité d'Hépatologie, Paris, France; Université Paris Descartes, INSERM U1223 and USM-20, Institut Pasteur, Paris, France.

Background: Although direct-acting antivirals have been used extensively to treat patients with chronic hepatitis C virus (HCV) infection, their clinical effectiveness has not been well reported. We compared the incidence of death, hepatocellular carcinoma, and decompensated cirrhosis between patients treated with direct-acting antivirals and those untreated, in the French ANRS CO22 Hepather cohort.

Methods: We did a prospective study in adult patients with chronic HCV infection enrolled from 32 expert hepatology centres in France. Read More

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http://dx.doi.org/10.1016/S0140-6736(18)32111-1DOI Listing
February 2019

Emerging Regulatory Roles of Dual-Specificity Phosphatases in Inflammatory Airway Disease.

Int J Mol Sci 2019 Feb 5;20(3). Epub 2019 Feb 5.

Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore.

Inflammatory airway disease, such as asthma and chronic obstructive pulmonary disease (COPD), is a major health burden worldwide. These diseases cause large numbers of deaths each year due to airway obstruction, which is exacerbated by respiratory viral infection. The inflammatory response in the airway is mediated in part through the MAPK pathways: p38, JNK and ERK. Read More

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http://dx.doi.org/10.3390/ijms20030678DOI Listing
February 2019