15,516 results match your criteria Journal of General Virology[Journal]


Evidence for the splicing of grablovirus transcripts reveals a putative novel open reading frame.

J Gen Virol 2019 Feb 18. Epub 2019 Feb 18.

Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University College of Agriculture and Life Sciences, Ithaca, NY, 14853, USA.

Grapevine red blotch virus (GRBV) is type member of the newly identified genus Grablovirus. It possesses a single-stranded circular DNA genome of around 3200 nucleotides encoding three open reading frames (ORFs) in both the virion sense, the V1 (CP), V2 and V3, and complementary sense, C1 (RepA), C2 and C3. As shown for members of the genus Mastrevirus, the C1 and C2 ORFs are predicted to fuse through splicing to form a replication-associated protein (Rep). Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001234DOI Listing
February 2019

Human SAMHD1 restricts the xenotransplantation relevant porcine endogenous retrovirus (PERV) in non-dividing cells.

J Gen Virol 2019 Feb 15. Epub 2019 Feb 15.

1​Department of HIV and other Retroviruses, Robert Koch Institute, Berlin, Germany.

The release of porcine endogenous retrovirus (PERV) particles from pig cells is a potential risk factor during xenotransplantation by way of productively infecting the human transplant recipient. Potential countermeasures against PERV replication are restriction factors that block retroviral replication. SAMHD1 is a triphosphohydrolase that depletes the cellular pool of dNTPs in non-cycling cells starving retroviral reverse transcription. Read More

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http://dx.doi.org/10.1099/jgv.0.001232DOI Listing
February 2019

Influenza virus entry via the GM3 ganglioside-mediated platelet-derived growth factor receptor β signalling pathway.

J Gen Virol 2019 Feb 14. Epub 2019 Feb 14.

1​Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium.

The possible resistance of influenza virus against existing antiviral drugs calls for new therapeutic concepts. One appealing strategy is to inhibit virus entry, in particular at the stage of internalization. This requires a better understanding of virus-host interactions during the entry process, including the role of receptor tyrosine kinases (RTKs). Read More

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http://dx.doi.org/10.1099/jgv.0.001235DOI Listing
February 2019

Combined infection with Tomato yellow leaf curl virus and Rickettsia influences fecundity, attraction to infected plants and expression of immunity-related genes in the whitefly Bemisia tabaci.

J Gen Virol 2019 Feb 14. Epub 2019 Feb 14.

1​Department of Entomology, The Volcani Center, Rishon LeZion, Israel.

We have recently shown that Rickettsia, a secondary facultative bacterial symbiont that infects the whitefly B. tabaci is implicated in the transmission of Tomato yellow leaf curl virus (TYLCV). Infection with Rickettsia improved the acquisition and transmission of the virus by B. Read More

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http://dx.doi.org/10.1099/jgv.0.001233DOI Listing
February 2019

Cytomegalovirus host entry and spread.

J Gen Virol 2019 Feb 7. Epub 2019 Feb 7.

School of Chemistry and Molecular Biosciences and Child Health Research Centre, University of Queensland, Brisbane, Australia.

Cytomegaloviruses (CMVs) are large, complex pathogens that persistently and systemically colonize most mammals. Human cytomegalovirus (HCMV) causes congenital harm, and has proved hard to control. One problem is that key vaccine targets - virus entry and spread in naive hosts - remain ill-defined. Read More

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http://dx.doi.org/10.1099/jgv.0.001230DOI Listing
February 2019

Host protein CD63 promotes viral RNA replication by interacting with human astrovirus non-structural protein nsP1a/4.

J Gen Virol 2019 Feb 6. Epub 2019 Feb 6.

1​College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121200, Liaoning, PR China.

Human astrovirus non-structural protein nsP1a/4, located at the C-terminal end of nsP1a, is thought to be involved in regulating RNA replication. Here, we show that host protein CD63 interacts with the nsP1a protein. Further research showed that the large loop (LEL) domain of CD63 also interacts with nsP1a/4. Read More

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http://dx.doi.org/10.1099/jgv.0.001231DOI Listing
February 2019

Hazara nairovirus elicits differential induction of apoptosis and nucleocapsid protein cleavage in mammalian and tick cells.

J Gen Virol 2019 Feb 5. Epub 2019 Feb 5.

1​School of Molecular and Cellular Biology, University of Leeds, Leeds, LS2 9JT, UK.

The Nairoviridae family within the Bunyavirales order comprise tick-borne segmented negative-sense RNA viruses that cause serious disease in a broad range of mammals, yet cause a latent and lifelong infection in tick hosts. An important member of this family is Crimean-Congo haemorrhagic fever virus (CCHFV), which is responsible for serious human disease that results in case fatality rates of up to 30 %, and which exhibits the most geographically broad distribution of any tick-borne virus. Here, we explored differences in the cellular response of both mammalian and tick cells to nairovirus infection using Hazara virus (HAZV), which is a close relative of CCHFV within the CCHFV serogroup. Read More

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http://dx.doi.org/10.1099/jgv.0.001211DOI Listing
February 2019

A single cycle influenza virus coated in H7 haemagglutinin generates neutralizing antibody responses to haemagglutinin and neuraminidase glycoproteins and protection from heterotypic challenge.

J Gen Virol 2019 Feb 4. Epub 2019 Feb 4.

1​MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK.

A non-replicating form of pseudotyped influenza virus, inactivated by suppression of the haemagglutinin signal sequence (S-FLU), can act as a broadly protective vaccine. S-FLU can infect for a single round only, and induces heterotypic protection predominantly through activation of cross-reactive T cells in the lung. Unlike the licensed live attenuated virus, it cannot reassort a pandemic haemagglutinin (HA) into seasonal influenza. Read More

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http://dx.doi.org/10.1099/jgv.0.001228DOI Listing
February 2019

Mutagenesis of the dengue virus NS4A protein reveals a novel cytosolic N-terminal domain responsible for virus-induced cytopathic effects and intramolecular interactions within the N-terminus of NS4A.

J Gen Virol 2019 Feb 1. Epub 2019 Feb 1.

1​Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan, PR China.

The NS4A protein of dengue virus (DENV) has a cytosolic N terminus and four transmembrane domains. NS4A participates in RNA replication and the host antiviral response. However, the roles of amino acid residues within the N-terminus of NS4A during the life cycle of DENV are not clear. Read More

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http://dx.doi.org/10.1099/jgv.0.001227DOI Listing
February 2019

Hepatitis E virus polymerase binds to IFIT1 to protect the viral RNA from IFIT1-mediated translation inhibition.

J Gen Virol 2019 Jan 31. Epub 2019 Jan 31.

2​Savitribai Phule Pune University, Ganeshkhind, Pune 411 007, India.

Hepatitis E virus (HEV) induces interferons and regulates the induction of interferon-stimulated genes (ISGs) in the host cell. HEV infection has been shown to promote the expression of different ISGs, such as ISG15, IFIT1, MX1, RSAD2/Viperin and CxCL10, in cell culture and animal models. Interferon-induced protein with tetratricopeptide repeat 1 (IFIT1) is an ISG-encoded protein that inhibits the translation of viral RNA, having 5'-triphosphate or the mRNA lacking 2'-O-methylation on the 5'cap. Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001229DOI Listing
January 2019
3 Reads

Effect of foot-and-mouth disease virus 3C protease B2 β-strand proline mutagenesis on expression and processing of the P1 polypeptide using a plasmid expression vector.

J Gen Virol 2019 Jan 31. Epub 2019 Jan 31.

3​U. S. Department of Homeland Security Science and Technology Directorate, Plum Island Animal Disease Center, Greenport, NY 11944, USA.

The production of experimental molecular vaccines against foot-and-mouth disease virus utilizes the viral encoded 3C protease for processing of the P1 polyprotein. Expression of wild type 3C protease is detrimental to host cells. The molecular vaccine constructs containing the 3C protease L127P mutant significantly reduce adverse effects associated with protease expression while retaining the ability to process and assemble virus-like particles. Read More

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http://dx.doi.org/10.1099/jgv.0.001204DOI Listing
January 2019
1 Read

Vehicles of intercellular communication: exosomes and HIV-1.

J Gen Virol 2019 Jan 31. Epub 2019 Jan 31.

4​Department of Pharmacologic Sciences, Basic Sciences Tower, Rm 8-142, Stony Brook, University School of Medicine, Stony Brook, NY 11794-8651, USA.

The terms extracellular vesicles, microvesicles, oncosomes, or exosomes are often used interchangeably as descriptors of particles that are released from cells and comprise a lipid membrane that encapsulates nucleic acids and proteins. Although these entities are defined based on a specific size range and/or mechanism of release, the terminology is often ambiguous. Nevertheless, these vesicles are increasingly recognized as important modulators of intercellular communication. Read More

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http://dx.doi.org/10.1099/jgv.0.001193DOI Listing
January 2019
1 Read

Characterization of ssDNA aptamers specifically directed against Trachinotus ovatus NNV (GTONNV)-infected cells with antiviral activities.

J Gen Virol 2019 Jan 30. Epub 2019 Jan 30.

3​Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, College of Marine Sciences, Guangxi University, Nanning, PR China.

Nervous necrosis virus (NNV), is one of the most fatal viruses in marine fish aquaculture, and is capable of infecting over 50 different fish species. Trachinotus ovatus NNV (GTONNV) was isolated from diseased golden pompano. This T. Read More

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http://dx.doi.org/10.1099/jgv.0.001226DOI Listing
January 2019

The baculovirus Ac108 protein is a per os infectivity factor and a component of the ODV entry complex.

J Gen Virol 2019 Jan 29. Epub 2019 Jan 29.

Laboratory of Virology, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Baculoviruses orally infect insect larvae when they consume viral occlusion bodies (OBs). OBs consist of a crystalline protein matrix in which the infectious virus particles, the occlusion-derived viruses (ODVs), are embedded. The protein matrix dissolves in the alkaline environment of the insect's midgut lumen. Read More

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http://dx.doi.org/10.1099/jgv.0.001200DOI Listing
January 2019

Transmembrane regions of bovine herpesvirus 1-encoded UL49.5 and glycoprotein M regulate complex maturation and ER-Golgi trafficking.

J Gen Virol 2019 Jan 29. Epub 2019 Jan 29.

1​Laboratory of Virus Molecular Biology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.

Bovine herpesvirus 1 (BoHV-1)-encoded UL49.5 (a homologue of herpesvirus glycoprotein N) can combine different functions, regulated by complex formation with viral glycoprotein M (gM). We aimed to identify the mechanisms governing the immunomodulatory activity of BoHV-1 UL49. Read More

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http://dx.doi.org/10.1099/jgv.0.001224DOI Listing
January 2019
1 Read

Identification of a novel membrane-associated protein from the S7 segment of grass carp reovirus.

J Gen Virol 2019 Jan 28. Epub 2019 Jan 28.

1​National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, PR China.

Aquareovirus is a genus of viruses in family Reoviridae, subfamily Spinareovirinae, members of which infect fish, shellfish and crustaceans. Grass carp reovirus (GCRV), a genotype 1 reovirus isolated from grass carp, has served as a model strain for investigating aquareovirus-host interactions. Herein, we report a neglected open reading frame (ORF), tentatively named NS12, residing between NS16 and NS31 in segment 7 (S7) of the virus genome. Read More

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http://dx.doi.org/10.1099/jgv.0.001223DOI Listing
January 2019

Characterization of Teviot virus, an Australian bat-borne paramyxovirus.

J Gen Virol 2019 Jan 28. Epub 2019 Jan 28.

1​CSIRO Health and Biosecurity, Australian Animal Health Laboratory, Geelong, Australia.

Bats are the reservoir hosts for multiple viruses with zoonotic potential, including coronaviruses, paramyxoviruses and filoviruses. Urine collected from Australian pteropid bats was assessed for the presence of paramyxoviruses. One of the viruses isolated was Teviot virus (TevPV), a novel rubulavirus previously isolated from pteropid bat urine throughout the east coast of Australia. Read More

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http://dx.doi.org/10.1099/jgv.0.001214DOI Listing
January 2019

Kodoja: A workflow for virus detection in plants using k-mer analysis of RNA-sequencing data.

J Gen Virol 2019 Jan 24. Epub 2019 Jan 24.

2​Information and Computational Sciences Group, The James Hutton Institute, Dundee, DD2 5DA, UK.

RNA-sequencing of plant material allows for hypothesis-free detection of multiple viruses simultaneously. This methodology relies on bioinformatics workflows for virus identification. Most workflows are designed for human clinical data, and few go beyond sequence mapping for virus identification. Read More

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http://dx.doi.org/10.1099/jgv.0.001210DOI Listing
January 2019
1 Read

Oncogenicitiy Comparison of Human Papillomavirus Type 52 E6 Variants.

J Gen Virol 2019 Jan 24. Epub 2019 Jan 24.

1​Department of Microbiology, Faulty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR.

Human papillomavirus (HPV) infection contributes to virtually all cases of cervical cancer, the fourth most common cancer affecting women worldwide. The oncogenicity of HPV is mainly attributable to the E6 and E7 oncoproteins. HPV-52 is the seventh most common HPV type globally, but it has a remarkably high prevalence in East Asia. Read More

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http://dx.doi.org/10.1099/jgv.0.001222DOI Listing
January 2019
1 Read

Increased predominance of HIV-1 CRF01_AE and its recombinants in the Philippines.

J Gen Virol 2019 Jan 24. Epub 2019 Jan 24.

1​Department of Medicine, Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA.

The growth rate of new HIV infections in the Philippines was the fastest of any countries in the Asia-Pacific region between 2010 and 2016. To date, HIV-1 subtyping results in the Philippines have been determined by characterizing only partial viral genome sequences. It is not known whether recombination occurs in the majority of unsequenced genome regions. Read More

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http://dx.doi.org/10.1099/jgv.0.001198DOI Listing
January 2019
1 Read

ICTV Virus Taxonomy Profile: Parvoviridae.

J Gen Virol 2019 Jan 23. Epub 2019 Jan 23.

12​Centre de Recherche de Microbiologie et Biotechnologie, INRS-Institut Armand-Frappier Laval, QC H7V 1B7, Canada.

Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, single-stranded DNA genomes of 4-6 kb. Viruses in two subfamilies, the Parvovirinae and Densovirinae, are distinguished primarily by their respective ability to infect vertebrates (including humans) versus invertebrates. Being genetically limited, most parvoviruses require actively dividing host cells and are host and/or tissue specific. Read More

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http://dx.doi.org/10.1099/jgv.0.001212DOI Listing
January 2019
1 Read
3.183 Impact Factor

The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NFκB-dependent promoter antagonism in chicken cells.

J Gen Virol 2019 Jan 23. Epub 2019 Jan 23.

1​The Pirbright Institute, Pirbright, Woking, UK.

The accessory protein, PB1-F2, of influenza A virus (IAV) functions in a chicken host to prolong infectious virus shedding and thus the transmission window. Here we show that this delay in virus clearance by PB1-F2 in chickens is accompanied by reduced transcript levels of type 1 interferon (IFN)-induced genes and NFκB-activated pro-inflammation cytokines. In vitro, two avian influenza isolate-derived PB1-F2 proteins, H9N2 UDL01 and H5N1 5092, exhibited the same antagonism of the IFN and pro-inflammation induction pathways seen in vivo, but to different extents. Read More

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http://dx.doi.org/10.1099/jgv.0.001220DOI Listing
January 2019
2 Reads

Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus - an unusual member of a putative new genus within the Potyviridae.

J Gen Virol 2019 Feb 22;100(2):308-320. Epub 2019 Jan 22.

1​Department Phytomedicine, Leibniz University Hannover, Institute of Horticultural Production Systems, Herrenhäuser Str. 2, 30419, Hannover, Germany.

Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5' untranslated region (UTR) of 13 nt and a 3' UTR consisting of 586 nt that is not polyadenylated. Read More

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http://dx.doi.org/10.1099/jgv.0.001207DOI Listing
February 2019
1 Read

Impact of naturally occurring variation in the human papillomavirus 52 capsid proteins on recognition by type-specific neutralising antibodies.

J Gen Virol 2019 Feb 18;100(2):237-245. Epub 2019 Jan 18.

1​Virus Reference Department, Public Health England, London, UK.

We investigated the impact of naturally occurring variation within the major (L1) and minor (L2) capsid proteins on the antigenicity of human papillomavirus (HPV) type 52 (HPV52). L1L2 pseudoviruses (PsVs) representing HPV52 lineage and sublineage variants A1, A2, B1, B2, C and D were created and tested against serum from naturally infected individuals, preclinical antisera raised against HPV52 A1 and D virus-like particles (VLPs) and neutralising monoclonal antibodies (MAbs) raised against HPV52 A1 VLP. HPV52 lineage D PsV displayed a median 3. Read More

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http://dx.doi.org/10.1099/jgv.0.001213DOI Listing
February 2019
1 Read

Analyses of four new Caulobacter Phicbkviruses indicate independent lineages.

J Gen Virol 2019 Feb 18;100(2):321-331. Epub 2019 Jan 18.

Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.

Bacteriophages with genomes larger than 200 kbp are considered giant phages, and the giant Phicbkviruses are the most frequently isolated Caulobacter crescentus phages. In this study, we compare six bacteriophage genomes that differ from the genomes of the majority of Phicbkviruses. Four of these genomes are much larger than those of the rest of the Phicbkviruses, with genome sizes that are more than 250 kbp. Read More

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http://dx.doi.org/10.1099/jgv.0.001218DOI Listing
February 2019
2 Reads

Two critical N-terminal epitopes of the nucleocapsid protein contribute to the cross-reactivity between porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus.

J Gen Virol 2019 Feb 17;100(2):206-216. Epub 2019 Jan 17.

3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China.

Both porcine epidemic diarrhoea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV), which cause high mortality in piglets and produce similar clinical symptoms and histopathological morphology, belong to the genus Alphacoronavirus. Serological diagnosis plays an important role in distinguishing pathogen species. Together with the spike (S) protein, the nucleocapsid (N) protein is one of the immunodominant regions among coronaviruses. Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001216DOI Listing
February 2019
3 Reads

Beyond sites 1 and 2, miR-122 target sites in the HCV genome have negligible contributions to HCV RNA accumulation in cell culture.

J Gen Virol 2019 Feb 17;100(2):217-226. Epub 2019 Jan 17.

2​Department of Biochemistry, McGill University, Montréal, QC, Canada.

Hepatitis C virus (HCV) recruits two molecules of the liver-specific microRNA-122 (miR-122) to two adjacent sites (S1 and S2) located at the 5' end of the viral RNA genome. This interaction promotes HCV RNA accumulation by stabilising the viral RNA and resulting in alteration of the secondary structure of the viral genome. In addition to S1 and S2, the HCV genome contains several other putative miR-122 binding sites, one in the IRES region, three in the NS5B coding region, and one in the 3' UTR. Read More

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http://dx.doi.org/10.1099/jgv.0.001217DOI Listing
February 2019
4 Reads

Gal power: the diverse roles of galectins in regulating viral infections.

J Gen Virol 2019 Jan 16. Epub 2019 Jan 16.

1​Discipline of Infectious Diseases and Immunology, University of Sydney, Camperdown, New South Wales, Australia.

Viruses, as a class of pathogenic microbe, remain a significant health burden globally. Viral infections result in significant morbidity and mortality annually and many remain in need of novel vaccine and anti-viral strategies. The development of effective novel anti-viral therapeutics, in particular, requires detailed understanding of the mechanism of viral infection, and the host response, including the innate and adaptive arms of the immune system. Read More

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http://dx.doi.org/10.1099/jgv.0.001208DOI Listing
January 2019
1 Read

Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.

J Gen Virol 2019 Feb 11;100(2):295-300. Epub 2019 Jan 11.

1​Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

The genomic organization and in vitro host range of a novel mosquito-associated orbivirus, designated Skunk River virus, is described. The virus was isolated from Aedes trivittatus collected in Iowa in the United States. Three recognized viruses were also recovered: Culex flavivirus (family Flaviviridae), Houston virus (family Mesoniviridae) and Umatilla virus (family Reoviridae). Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001219DOI Listing
February 2019
5 Reads

Mosquito-borne epornitic flaviviruses: an update and review.

J Gen Virol 2019 Feb 22;100(2):119-132. Epub 2019 Jan 22.

1​FARAH Research Center, Department of Pathology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43, B-4000 Liège, Belgium.

West Nile Virus, Usutu virus, Bagaza virus, Israel turkey encephalitis virus and Tembusu virus currently constitute the five flaviviruses transmitted by mosquito bites with a marked pathogenicity for birds. They have been identified as the causative agents of severe neurological symptoms, drop in egg production and/or mortalities among avian hosts. They have also recently shown an expansion of their geographic distribution and/or a rise in cases of human infection. Read More

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http://dx.doi.org/10.1099/jgv.0.001203DOI Listing
February 2019
3 Reads

Occurrence of deformed wing virus variants in the stingless bee Melipona subnitida and honey bee Apis mellifera populations in Brazil.

J Gen Virol 2019 Feb 10;100(2):289-294. Epub 2019 Jan 10.

1​School of Environment and Life Sciences, The University of Salford, Manchester M5 4WT, UK.

The global spread of the parasitic Varroa mite has introduced a new bee to the bee horizontal transmission route for several RNA viruses that bypasses existing barriers in honey bees. From among these viruses, deformed wing virus (DWV) is now among the most widespread insect pathogens in the world. Brazilian stingless bees are a diverse group often managed in close proximity to honey bees. Read More

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http://dx.doi.org/10.1099/jgv.0.001206DOI Listing
February 2019
1 Read

Identification of T-cell epitopes in African swine fever virus CD2v and C-type lectin proteins.

J Gen Virol 2019 Feb 10;100(2):259-265. Epub 2019 Jan 10.

5​Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.

African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available, and progress is hindered by lack of knowledge concerning the extent of ASF virus (ASFV) strain diversity and the viral antigens conferring type-specific protective immunity in pigs. Previously, we demonstrated that ASFV serotype-specific proteins CD2v (EP402R) and/or C-type lectin (EP153R) are important for protection against homologous ASF infection. Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001195DOI Listing
February 2019
19 Reads

Defining of MAbs-neutralizing sites on the surface glycoproteins Gn and Gc of a hantavirus using vesicular stomatitis virus pseudotypes and site-directed mutagenesis.

J Gen Virol 2019 Feb 9;100(2):145-155. Epub 2019 Jan 9.

1​Department of Virology, University of Helsinki, Medicum, Helsinki, Finland.

Earlier four monoclonal antibodies (MAbs) against surface glycoproteins Gn and Gc of puumala virus (PUUV, genus Orthohantavirus, family Hantaviridae, order Bunyavirales) were generated and for three MAbs with neutralizing capacity the localization of binding epitopes was predicted using pepscan and phage-display techniques. In this work, we produced vesicular stomatitis virus (VSV) particles pseudotyped with the Gn and Gc glycoproteins of PUUV and applied site-directed mutagenesis to dissect the structure of neutralizing epitopes. Replacement of cysteine amino acid (aa) residues with alanines resulted in pseudotype particles with diminished (16 to 18 %) neut-titres; double Cys→Ala mutants, as well as mutants with bulky aromatic and charged residues replaced with alanines, have shown even stronger reduction in neut-titres (from 25 % to the escape phenotype). Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001202DOI Listing
February 2019
2 Reads

Experimental infection of Japanese macaques with simian retrovirus 5.

J Gen Virol 2019 Feb 4;100(2):266-277. Epub 2019 Jan 4.

1​Laboratory of Virus-Host Coevolution, Research Center for Infectious Diseases, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.

Recently, a large number of Japanese macaques (Macaca fuscata) died of an unknown hemorrhagic syndrome at Kyoto University Primate Research Institute (KUPRI) and an external breeding facility for National Institute for Physiological Sciences (NIPS). We previously reported that the hemorrhagic syndrome of Japanese macaques at KUPRI was caused by infection with simian retrovirus 4 (SRV-4); however, the cause of similar diseases that occurred at the external breeding facility for NIPS was still unknown. In this study, we isolated SRV-5 from Japanese macaques exhibiting thrombocytopenia and then constructed an infectious molecular clone of the SRV-5 isolate. Read More

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http://dx.doi.org/10.1099/jgv.0.001199DOI Listing
February 2019
1 Read

The antiviral activity of rodent and lagomorph SERINC3 and SERINC5 is counteracted by known viral antagonists.

J Gen Virol 2019 Feb 19;100(2):278-288. Epub 2018 Dec 19.

5​Institute of Virology, Technische Universität München/Helmholtz Zentrum, Munich, Germany.

A first step towards the development of a human immunodeficiency virus (HIV) animal model has been the identification and surmounting of species-specific barriers encountered by HIV along its replication cycle in cells from small animals. Serine incorporator proteins 3 (SERINC3) and 5 (SERINC5) were recently identified as restriction factors that reduce HIV-1 infectivity. Here, we compared the antiviral activity of SERINC3 and SERINC5 among mice, rats and rabbits, and their susceptibility to viral counteraction to their human counterparts. Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001201DOI Listing
February 2019
5 Reads

Role of APOBEC3 proteins in proteasome inhibitor-mediated reactivation of latent HIV-1 viruses.

J Gen Virol 2018 Dec 19. Epub 2018 Dec 19.

Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi-110021, Delhi.

Proteasome inhibitors (PIs) have been identified as an emerging class of HIV-1 latency-reversing agents (LRAs). These inhibitors can reactivate latent HIV-1 to produce non-infectious viruses. The mechanism underlying reduced infectivity of reactivated viruses is unknown. Read More

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http://dx.doi.org/10.1099/jgv.0.001205DOI Listing
December 2018
1 Read
3.183 Impact Factor

Characterization of Triniti virus supports its reclassification in the family Peribunyaviridae.

J Gen Virol 2019 Feb 14;100(2):137-144. Epub 2018 Dec 14.

1​Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, S/N - 67030-000 - Levilândia - Ananindeua, Pará, Brazil.

Triniti virus (TNTV) has been isolated in Trinidad and Tobago and in Brazil. To date little is known about this virus, which is classified as an ungrouped virus within the family Togaviridae. Here, three isolates of TNTV were characterized both genetically and antigenically. Read More

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http://dx.doi.org/10.1099/jgv.0.001196DOI Listing
February 2019
3 Reads

Potent neutralization activity against type O foot-and-mouth disease virus elicited by a conserved type O neutralizing epitope displayed on bovine parvovirus virus-like particles.

J Gen Virol 2019 Feb 14;100(2):187-198. Epub 2018 Dec 14.

Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Street, Harbin 150069, PR China.

In this study, ten sites on the N terminus and different surface variable regions (VRs) of the bovine parvovirus (BPV) VP2 capsid protein were selected according to an alignment of its sequence with that of the BPV-1 strain HADEN for insertion of the type O foot-and-mouth disease virus (FMDV) conserved neutralizing epitope 8E8. Ten epitope-chimeric BPV VP2 capsid proteins carrying the 8E8 epitope were expressed in Sf9 cells, and electron micrographs demonstrated that these fusion proteins self-assembled into virus-like particles (VLPs) with properties similar to those of natural BPV virions. Immunofluorescence assay (IFA) and Western blot analysis demonstrated that each of the ten epitope-chimeric VLPs reacted with both anti-BPV serum and anti-type O FMDV mAb 8E8. Read More

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http://dx.doi.org/10.1099/jgv.0.001194DOI Listing
February 2019
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ICTV Virus Taxonomy Profile: Tristromaviridae.

J Gen Virol 2019 Feb 12;100(2):135-136. Epub 2018 Dec 12.

1​Department of Microbiology, Institut Pasteur, 25 Rue du Dr. Roux, 75015 Paris, France.

Tristromaviridae is a family of viruses with linear, double-stranded DNA genomes of 16-18 kbp. The flexible, filamentous virions (400±20 nm×30±3 nm) consist of an envelope and an inner core constructed from two structural units: a rod-shaped helical nucleocapsid and a nucleocapsid-encompassing matrix protein layer. Tristromaviruses are lytic and infect hyperthermophilic archaea of the order Thermoproteales. Read More

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http://dx.doi.org/10.1099/jgv.0.001190DOI Listing
February 2019
2 Reads

Proteomic analysis of the Heliothis virescens ascovirus 3i (HvAV-3i) virion.

J Gen Virol 2019 Feb 12;100(2):301-307. Epub 2018 Dec 12.

2​Institute of Virology, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan 410128, PR China.

Ascoviruses are enveloped, circular, double-stranded DNA viruses that can effectively control the appetite of lepidopteran larvae, thereby reducing the consequent damage and economic losses to crops. In this study, the virion of a sequenced Heliothis virescens ascovirus 3i (HvAV-3i) strain was used to perform proteomic analysis using both in-gel and in-solution digestion. A total of 81 viral proteins, of which 67 were associated with the virions, were identified in the proteome of HvAV-3i virions. Read More

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http://dx.doi.org/10.1099/jgv.0.001197DOI Listing
February 2019
2 Reads

Co-circulation of highly diverse Aleutian mink disease virus strains in Finland.

J Gen Virol 2019 Feb 10;100(2):227-236. Epub 2018 Dec 10.

1​Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin katu 2, 00790, Helsinki, Finland.

Aleutian mink disease virus (AMDV) is the causative agent of Aleutian disease (AD), which affects mink of all genotypes and also infects other mustelids such as ferrets, martens and badgers. Previous studies have investigated diversity in Finnish AMDV strains, but these studies have been restricted to small parts of the virus genome, and mostly from newly infected farms and free-ranging mustelids. Here, we investigated the diversity and evolution of Finnish AMDV strains by sequencing the complete coding sequences of 31 strains from mink originating from farms differing in their virus history, as well as from free-ranging mink. Read More

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http://dx.doi.org/10.1099/jgv.0.001187DOI Listing
February 2019
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Antibody arrests γ-herpesvirus olfactory super-infection independently of neutralization.

J Gen Virol 2019 Feb 10;100(2):246-258. Epub 2018 Dec 10.

3​Royal Children's Hospital, Brisbane, Australia.

Protecting against persistent viruses is an unsolved challenge. The clearest example for a gamma-herpesvirus is resistance to super-infection by Murid herpesvirus-4 (MuHV-4). Most experimental infections have delivered MuHV-4 into the lungs. Read More

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http://dx.doi.org/10.1099/jgv.0.001183DOI Listing
February 2019
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Temperature-sensitive origin-binding protein as a tool for investigations of herpes simplex virus activities in vivo.

J Gen Virol 2019 Jan 6;100(1):105-117. Epub 2018 Dec 6.

Institute of Virology, University of Bonn, Bonn, Germany.

While it is fairly clear that herpes simplex virus (HSV) DNA replication requires at least seven virus-encoded proteins in concert with various host cell factors, the mode of this process in infected cells is still poorly understood. Using HSV-1 mutants bearing temperature-sensitive (ts) lesions in the UL9 gene, we previously found that the origin-binding protein (OBP), a product of the UL9 gene, is only needed in the first 6 hours post-infection. As this finding was just a simple support for the hypothesis of a biphasic replication mode, we became convinced through these earlier studies that the mutants tsR and tsS might represent suitable tools for more accurate investigations in vivo. Read More

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http://dx.doi.org/10.1099/jgv.0.001184DOI Listing
January 2019
2 Reads

Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.

J Gen Virol 2019 Feb 3;100(2):199-205. Epub 2018 Dec 3.

1​Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK.

The substitution rates of viral polymerases have been studied extensively. However less is known about the tendency of these enzymes to 'slip' during RNA synthesis to produce progeny RNAs with nucleotide insertions or deletions. We recently described the functional utilization of programmed polymerase slippage in the family Potyviridae. Read More

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http://dx.doi.org/10.1099/jgv.0.001189DOI Listing
February 2019
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ICTV virus taxonomy profile: Sarthroviridae.

J Gen Virol 2018 Dec;99(12):1563-1564

5​Department of Biological Sciences, University of Texas at El Paso, El Paso, TX 79968, USA.

The family Sarthroviridae includes a single genus, Macronovirus, which in turn includes a single species, Macrobrachium satellite virus 1. Members of this species, named extra small virus, are satellite viruses of Macrobrachium rosenbergii nodavirus, an unclassified virus related to members of the family Nodaviridae. Both viruses have isometric, spherical virions, infect giant freshwater prawns and together cause white tail disease, which is responsible for mass mortalities and severe economic losses in hatcheries and farms. Read More

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http://dx.doi.org/10.1099/jgv.0.001158DOI Listing
December 2018
2 Reads

DORA Editorial.

J Gen Virol 2019 Jan 5;100(1):1-2. Epub 2018 Dec 5.

6​Wellcome Sanger Institute, Cambridge, UK.

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http://dx.doi.org/10.1099/jgv.0.001191DOI Listing
January 2019
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Prevalence and genome characteristics of atypical porcine pestivirus in southwest China.

J Gen Virol 2019 Jan 5;100(1):84-88. Epub 2018 Dec 5.

3​Animal Disease Prevention and Control Innovation Team in the Qinghai-Tibetan Plateau of State Ethnic Affairs Commission, Chengdu 610041, PR China.

Atypical porcine pestivirus (APPV) causes congenital tremor (CT) in piglets and has a wide geographical distribution. In this study, we evaluated APPV prevalence using 165 piglet sera from southwest China. Viral RNA was detectable by qRT-PCR in 43. Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001188DOI Listing
January 2019
14 Reads

Hepatitis C virus NS5A inhibitor daclatasvir allosterically impairs NS4B-involved protein-protein interactions within the viral replicase and disrupts the replicase quaternary structure in a replicase assembly surrogate system.

J Gen Virol 2019 Jan 5;100(1):69-83. Epub 2018 Dec 5.

2​Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Fudan University, Shanghai, PR China.

Daclatasvir (DCV) is a highly potent direct-acting antiviral that targets the non-structural protein 5A (NS5A) of hepatitis C virus (HCV) and has been used with great clinical success. Previous studies have demonstrated its impact on viral replication complex assembly. However, the precise mechanisms by which DCV impairs the replication complex assembly remains elusive. Read More

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http://dx.doi.org/10.1099/jgv.0.001180DOI Listing
January 2019
6 Reads

Membrane trafficking RNA interference screen identifies a crucial role of the clathrin endocytic pathway and ARP2/3 complex for Japanese encephalitis virus infection in HeLa cells.

J Gen Virol 2019 Feb 29;100(2):176-186. Epub 2018 Nov 29.

1​Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana, India.

Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is one of the leading global causes of virus-induced encephalitis. The infectious life-cycle of viruses is heavily dependent on the host membrane trafficking network. Here, we have performed a RNA-interference-based screen using a siRNA panel targeting 136 membrane trafficking proteins to identify the key regulators of JEV infection in HeLa cells. Read More

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http://dx.doi.org/10.1099/jgv.0.001182DOI Listing
February 2019
2 Reads