15,541 results match your criteria Journal of General Virology[Journal]


Population dynamics at neuraminidase position 151 of influenza A (H1N1)pdm09 virus in clinical specimens.

J Gen Virol 2019 Apr 17. Epub 2019 Apr 17.

3​Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC.

Influenza A virus mutates rapidly, allowing it to escape natural and vaccine-induced immunity. Neuraminidase (NA) is a surface protein capable of cleaving the glycosidic linkages of neuraminic acids to release newly formed virions from infected cells. Genetic variants within a viral population can influence the emergence of pandemic viruses as well as drug susceptibility and vaccine effectiveness. Read More

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http://dx.doi.org/10.1099/jgv.0.001258DOI Listing

Identification of novel epitopes in serotype O foot-and-mouth disease virus by in vitro immune selection.

J Gen Virol 2019 Apr 16. Epub 2019 Apr 16.

The Pirbright Institute, Ash Road, Woking, Surrey, GU24 0NF, UK.

Foot-and-mouth disease virus (FMDV) displays various epitopes on the capsid outer surface. In addition to the five neutralizing antigenic sites, there is evidence of the existence of other, yet unidentified, epitopes that are believed to play a role in antibody-mediated protection. Previous attempts to identify these epitopes revealed two additional substitutions at positions VP2-74 and -191 (5M2/5 virus) to be of antigenic significance. Read More

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http://dx.doi.org/10.1099/jgv.0.001259DOI Listing

Distinct replication and gene expression strategies of the Rice Stripe virus in vector insects and host plants.

J Gen Virol 2019 Apr 16. Epub 2019 Apr 16.

1​State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.

Persistent propagative plant viruses are usually transmitted between a vector insect and a host plant. To adapt to the two different organisms, viruses may show distinct genomic replication or gene expression patterns. To verify this hypothesis, we applied an aboslute real-time quantitative PCR method to measure and compare the replication levels of four genomic RNA segments and the expression levels of seven genes of rice stripe virus (RSV) according to the infection time in the small brown planthopper and rice plant, respectively. Read More

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https://www.microbiologyresearch.org/content/journal/jgv/10.
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http://dx.doi.org/10.1099/jgv.0.001255DOI Listing
April 2019
1 Read

Novel subgenotype D11 of hepatitis B virus in NaPo County, Guangxi, bordering Vietnam.

J Gen Virol 2019 Apr 16. Epub 2019 Apr 16.

1​Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Guangxi Key Laboratory for the Prevention and Control of Viral Hepatitis, Nanning, Guangxi 530028, PR China.

Hepatitis B virus has been classified into 10 genotypes and 48 subgenotypes worldwide. We found previously, through polymerase chain reaction (PCR) amplification of a sample collected in 2011, that an HBsAg carrier was infected with two genotypes (B and D) of HBV. We carried out cloning, sequencing and phylogenetic analysis of the complete genomes and, for confirmation, analysed a sample collected from the same individual in 2018. Read More

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http://dx.doi.org/10.1099/jgv.0.001257DOI Listing
April 2019
2 Reads

In praise of preprints.

J Gen Virol 2019 Apr 2. Epub 2019 Apr 2.

3​Microbiology Society, London, UK.

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http://dx.doi.org/10.1099/jgv.0.001239DOI Listing

First detection of bovine coronavirus in Yak (Bos grunniens) and a bovine coronavirus genome with a recombinant HE gene.

J Gen Virol 2019 Apr 1. Epub 2019 Apr 1.

2​Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation, Chengdu, PR China.

The yak (Bosgrunniens) is a unique domestic bovine species that plays an indispensable role for herdsmen in the Qinghai-Tibet Plateau. Here, 336 diarrhoeic samples were collected from yaks on 29 farms in the Qinghai-Tibet Plateau from 2015 to 2017. Approximately 69. Read More

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http://dx.doi.org/10.1099/jgv.0.001254DOI Listing

Secondary structure of DNA released from purified capsids of human parvovirus B19 under moderate denaturing conditions.

J Gen Virol 2019 Mar 29. Epub 2019 Mar 29.

1​Institute of Virology, University of Bonn Medical Centre, Germany.

Parvovirus B19 (B19V) possesses a linear single-stranded DNA genome of either positive or negative polarity. Due to intramolecular sequence homologies, either strand may theoretically be folded in several alternative ways. Viral DNA, when extracted from virions by several procedures, presents as linear single-stranded and/or linear double-stranded molecules, except when one particular commercial kit is used. Read More

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http://dx.doi.org/10.1099/jgv.0.001253DOI Listing

ICTV Virus Taxonomy Profile: Nimaviridae.

J Gen Virol 2019 Mar 29. Epub 2019 Mar 29.

6​Pathology and Molecular Systematics Team, Centre for Environment, Fisheries andAquaculture Science (Cefas), Weymouth, DT4 8UB, UK.

The family Nimaviridae includes the single species White spot syndrome virus, isolates of which infect a wide range of aquatic crustaceans and cause substantial economic losses. Virions are ellipsoid to bacilliform with a terminal thread-like extension. The circular dsDNA genome is 280-307 kbp with several homologous repeat regions. Read More

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http://dx.doi.org/10.1099/jgv.0.001248DOI Listing
March 2019
1 Read

Influenza virus infection as well as immunization with DNA encoding haemagglutinin protein induces potent antibody-dependent phagocytosis (ADP) and monocyte infection-enhancing responses in macaques.

J Gen Virol 2019 Mar 28. Epub 2019 Mar 28.

1​Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.

Antibodies directed against the conserved regions within the influenza A haemagglutinin (HA) protein are detected at low frequency in humans. These antibodies display a broad reactivity against divergent influenza virus strains and could potentially offer broad protection. The in vivo protective effect of these antibodies is mainly mediated through Fc receptor effector functions. Read More

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http://dx.doi.org/10.1099/jgv.0.001251DOI Listing

Upregulation of CHOP participates in caspase activation and virus release in human astrovirus-infected cells.

J Gen Virol 2019 Mar 26. Epub 2019 Mar 26.

4​Laboratory of Radiation Safety, National Center for Geriatrics and Gerontology, Obu, Aichi, 474-8522, Japan.

Human astroviruses (HAstVs), non-enveloped RNA viruses with positive-sense RNA genomes, are an important cause of acute gastroenteritis in young children, although the processes that produce infectious virions are not clearly defined. To track the viral replication complex (RC) upon HAstV1 infection, the subcellular distribution of double-stranded (ds) RNA and of ORF1b, a viral RNA polymerase, was examined by immunocytochemistry. Foci that were positive for dsRNA and for ORF1b were co-localized, and both foci were also co-localized with resident proteins of the endoplasmic reticulum (ER). Read More

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http://dx.doi.org/10.1099/jgv.0.001250DOI Listing
March 2019
1 Read
3.183 Impact Factor

IRF4 promotes Epstein-Barr virus activation in Burkitt's lymphoma cells.

J Gen Virol 2019 Mar 25. Epub 2019 Mar 25.

2​University of Chinese Academy of Sciences, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, PR China.

Epstein-Barr virus (EBV) establishes a life-long latency in memory B cells, whereas plasma cell differentiation is linked to EBV lytic reactivation from latently infected B cells. EBV lytic replication is mediated by the two immediate-early switch proteins Zta and RTA. Both plasma cell transcription factors XBP-1 and Blimp-1 have been shown to enable the triggering of EBV lytic reactivation by activating the transcription of Zta or RTA. Read More

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http://dx.doi.org/10.1099/jgv.0.001249DOI Listing
March 2019
8 Reads
3.183 Impact Factor

Characterization of Fowlpox virus in chickens and bird-biting mosquitoes: a molecular approach to investigating Avipoxvirus transmission.

J Gen Virol 2019 Mar 25. Epub 2019 Mar 25.

1​Environmental Health Institute, National Environment Agency, 11, Biopolis Way, #06-05-08, Singapore 138667, Singapore.

Avian pox is a highly contagious avian disease, yet relatively little is known about the epidemiology and transmission of Avipoxviruses. Using a molecular approach, we report evidence for a potential link between birds and field-caught mosquitoes in the transmission of Fowlpox virus (FWPV) in Singapore. Comparison of fpv167 (P4b), fpv126 (VLTF-1), fpv175-176 (A11R-A12L) and fpv140 (H3L) gene sequences revealed close relatedness between FWPV strains obtained from cutaneous lesions of a chicken and four pools of Culex pseudovishnui, Culex spp. Read More

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http://dx.doi.org/10.1099/jgv.0.001209DOI Listing
March 2019
3.183 Impact Factor

Detection of antisense protein (ASP) RNA transcripts in individuals infected with human immunodeficiency virus type 1 (HIV-1).

J Gen Virol 2019 Mar 21. Epub 2019 Mar 21.

1​Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.

The detection of antisense RNA is hampered by reverse transcription (RT) non-specific priming, due to the ability of RNA secondary structures to prime RT in the absence of specific primers. The detection of antisense RNA by conventional RT-PCR does not allow assessment of the polarity of the initial RNA template, causing the amplification of non-specific cDNAs. In this study we have developed a modified protocol for the detection of human immunodeficiency virus type 1 (HIV-1) antisense protein (ASP) RNA. Read More

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http://dx.doi.org/10.1099/jgv.0.001244DOI Listing
March 2019
1 Read

A recombinant canine distemper virus expressing interleukin-7 enhances humoral immunity.

J Gen Virol 2019 Apr 15;100(4):602-615. Epub 2019 Mar 15.

3​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.

Canine distemper (CD) causes gastrointestinal and respiratory and/or neurological signs and results in high morbidity and mortality, remaining a threat to carnivores around the world. Live-attenuated vaccines have been widely used to reduce the number of CD outbreaks, but efforts are still needed to improve immune efficiency. Interleukin-7 (IL-7) has been reported to boost host immunity by recruiting follicle helper T (TFH) or germinal center (GC) B cells. Read More

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http://dx.doi.org/10.1099/jgv.0.001247DOI Listing
April 2019
1 Read

In vitro and in vivo roles of sphingosine kinase 2 during dengue virus infection.

J Gen Virol 2019 Apr 14;100(4):629-641. Epub 2019 Mar 14.

1​Microbiology and Infectious Diseases, College of Medicine and Public Health, Flinders University, Bedford Park, Adelaide, 5042, South Australia.

There is growing evidence of the influence of sphingosine kinase (SK) enzymes on viral infection. Here, the role of sphingosine kinase 2 (SK2), an isoform of SK prominent in the brain, was defined during dengue virus (DENV) infection. Chemical inhibition of SK2 activity using two different SK2 inhibitors, ABC294640 and K145, had no effect on DENV infection in human cells in vitro. Read More

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http://dx.doi.org/10.1099/jgv.0.001245DOI Listing

A novel species of RNA virus associated with root lesion nematode Pratylenchus penetrans.

J Gen Virol 2019 Apr 14;100(4):704-708. Epub 2019 Mar 14.

1​Molecular Plant Pathology Laboratory, Agricultural Research Service, USA Department of Agriculture, Beltsville, USA.

The root lesion nematode Pratylenchus penetrans is a migratory species that attacks a broad range of plants. While analysing transcriptomic datasets of P. penetrans, we have identified a full-length genome of an unknown positive-sense single-stranded RNA virus, provisionally named root lesion nematode virus 1 (RLNV1). Read More

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http://dx.doi.org/10.1099/jgv.0.001246DOI Listing

Evaluation of 2012 US EHDV-2 outbreak isolates for genetic determinants of cattle infection.

J Gen Virol 2019 Apr 14;100(4):556-567. Epub 2019 Mar 14.

1​United States Department of Agriculture, Agricultural Research Service, Arthropod-borne Animal Diseases Research Unit, 1515 College Avenue, Manhattan, KS 66502, USA.

Following a summer of severe drought and abnormally high temperatures, a major outbreak of EHDV occurred during 2012 in the USA. Although EHDV-1, -2 and -6 were isolated, EHDV-2 was the predominant virus serotype detected during the outbreak. In addition to large losses of white-tailed deer, the Midwest and northern Plains saw a significant amount of clinical disease in cattle. Read More

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http://dx.doi.org/10.1099/jgv.0.001221DOI Listing

A low-passage insect-cell isolate of bluetongue virus uses a macropinocytosis-like entry pathway to infect natural target cells derived from the bovine host.

J Gen Virol 2019 Apr 7;100(4):568-582. Epub 2019 Mar 7.

2​University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Bluetongue virus (BTV) causes an economically important disease in domestic and wildlife ruminants and is transmitted by Culicoides biting midges. In ruminants, BTV has a wide cell tropism that includes endothelial cells of vascular and lymphatic vessels as important cell targets for virus replication, and several cell types of the immune system including monocytes, macrophages and dendritic cells. Thus, cell-entry represents a particular challenge for BTV as it infects many different cell types in widely diverse vertebrate and invertebrate hosts. Read More

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http://dx.doi.org/10.1099/jgv.0.001240DOI Listing
April 2019
2 Reads
3.183 Impact Factor

ICTV Virus Taxonomy Profile: Polycipiviridae.

J Gen Virol 2019 Apr 5;100(4):554-555. Epub 2019 Mar 5.

3​United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.

Polycipiviridae is a family of picorna-like viruses with non-segmented, linear, positive-sense RNA genomes of approximately 10-12 kb. Unusually for viruses within the order Picornavirales, their genomes are polycistronic, with four (or more) consecutive 5'-proximal open reading frames (ORFs) encoding structural (and possibly other) proteins and a long 3' ORF encoding the replication polyprotein. Members of species within the family have all been detected in ants or via arthropod transcriptomic datasets. Read More

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https://www.microbiologyresearch.org/content/journal/jgv/10.
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http://dx.doi.org/10.1099/jgv.0.001241DOI Listing
April 2019
9 Reads
3.183 Impact Factor

ICTV Virus Taxonomy Profile: Solinviviridae.

J Gen Virol 2019 Mar 5. Epub 2019 Mar 5.

3​United States Department of Agriculture, Agricultural Research Service, Beltsville MD 20705, USA.

Solinviviridae is a family of picorna/calici-like viruses with non-segmented, linear, positive-sense RNA genomes of approximately 10-11 kb. Unusually, their capsid proteins are encoded towards the 3'-end of the genome where they can be expressed both from a subgenomic RNA and as an extension of the replication (picorna-like helicase-protease-polymerase) polyprotein. Members of two species within the family infect ants, but related unclassified virus sequences derive from a large variety of insects and other arthropods. Read More

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http://dx.doi.org/10.1099/jgv.0.001242DOI Listing
March 2019
1 Read
3.183 Impact Factor

Prediction of the molecular boundary and functionality of novel viral AlkB domains using homology modelling and principal component analysis.

J Gen Virol 2019 Apr 5;100(4):691-703. Epub 2019 Mar 5.

Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.

Alkylation B (AlkB) proteins are ubiquitous among diverse cellular organisms, where they act to reverse the damage in DNA and RNA due to methylation, such as 1-methyladenine and 3-methylcytosine. This process is found in virtually all forms of life, with the notable exception of archaea and yeast. This protein family is so significant to all forms of life that it was recently discovered that an AlkB domain is encoded as part of the replicase (poly)protein in a small subset of single-stranded, positive-sense RNA viruses, mainly belonging to the families Alphaflexiviridae, Betaflexiviridae and Closteroviridae. Read More

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http://dx.doi.org/10.1099/jgv.0.001237DOI Listing
April 2019
7 Reads

Defining early events of Epstein-Barr virus (EBV) infection in immortalized nasopharyngeal epithelial cells using cell-free EBV infection.

J Gen Virol 2019 Feb 28. Epub 2019 Feb 28.

4​School of Nursing, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, PR China.

Epstein-Barr virus (EBV) infection is strongly associated with nasopharyngeal carcinoma, a common cancer in Southeast Asia and certain regions of Africa. However, the dynamics of EBV episome maintenance in infected nasopharyngeal epithelial (NPE) cells remain largely undefined. Here, we report the establishment of a highly efficient cell-free EBV infection method for NPE cells. Read More

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http://dx.doi.org/10.1099/jgv.0.001243DOI Listing
February 2019

Acquisition of a phospho-acceptor site enhances HPV E6 PDZ-binding motif functional promiscuity.

J Gen Virol 2019 Feb 27. Epub 2019 Feb 27.

International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34149 Trieste, Italy.

All cancer-causing human papillomavirus (HPV) E6 oncoproteins have a C-terminal PDZ-binding motif (PBM), which correlates with oncogenic potential. Nonetheless, several HPVs with little or no oncogenic potential also have an E6 PBM, with minor sequence differences affecting PDZ protein selectivity. Furthermore, certain HPV types have a phospho-acceptor site embedded within the PBM. Read More

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http://dx.doi.org/10.1099/jgv.0.001236DOI Listing
February 2019
1 Read

Elucidating the genetic diversity of Phthorimaea operculella granulovirus (PhopGV).

J Gen Virol 2019 Apr 22;100(4):679-690. Epub 2019 Feb 22.

Institute for Biological Control, Federal Research Centre for Cultivated Plants, Julius Kühn Institute, Heinrichstraße 243, 64287 Darmstadt, Germany.

Twelve complete genome sequences of Phthorimaea operculella granulovirus (PhopGV) isolates from four different continents (Africa, South America, Asia and Europe) were analysed after Illumina next-generation sequencing (NGS). The isolates have a circular double-stranded DNA genome that is 118 355 to 119 177 bp in length and all of them encode 130 open reading frames (ORFs). Analysis of single-nucleotide polymorphisms (SNPs) revealed a unique set of SNP positions for every tested isolate. Read More

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http://dx.doi.org/10.1099/jgv.0.001215DOI Listing
April 2019
1 Read

Identification of a post-transcriptional regulatory element in the human endogenous retroviral syncytin-1.

J Gen Virol 2019 Apr 22;100(4):662-668. Epub 2019 Feb 22.

Laboratory of Virus-Host Coevolution, Institute for Frontier Life and Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.

Retroviral transcripts have cis-acting elements that interact with host and viral proteins to enable efficient nuclear export and/or translation; however, it is poorly understood whether the transcripts of human endogenous retroviral genes retain such elements. Here, we show that human syncytin-1, which is derived from human endogenous retrovirus W, requires a 3' untranslated region (3'UTR) for efficient gene expression and retains a post-transcriptional regulatory element (named SPRE). The insertion of SPRE markedly increased a reporter gene (human immunodeficiency virus type 1 Gag) expression without affecting the amounts of nuclear or cytoplasmic transcript. Read More

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http://dx.doi.org/10.1099/jgv.0.001238DOI Listing

Evidence for the splicing of grablovirus transcripts reveals a putative novel open reading frame.

J Gen Virol 2019 Apr 18;100(4):709-720. Epub 2019 Feb 18.

Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University College of Agriculture and Life Sciences, Ithaca, NY, 14853, USA.

Grapevine red blotch virus (GRBV) is type member of the newly identified genus Grablovirus. It possesses a single-stranded circular DNA genome of around 3200 nucleotides encoding three open reading frames (ORFs) in both the virion sense, the V1 (CP), V2 and V3, and complementary sense, C1 (RepA), C2 and C3. As shown for members of the genus Mastrevirus, the C1 and C2 ORFs are predicted to fuse through splicing to form a replication-associated protein (Rep). Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001234DOI Listing

Human SAMHD1 restricts the xenotransplantation relevant porcine endogenous retrovirus (PERV) in non-dividing cells.

J Gen Virol 2019 Apr 15;100(4):656-661. Epub 2019 Feb 15.

1​Department of HIV and other Retroviruses, Robert Koch Institute, Berlin, Germany.

The release of porcine endogenous retrovirus (PERV) particles from pig cells is a potential risk factor during xenotransplantation by way of productively infecting the human transplant recipient. Potential countermeasures against PERV replication are restriction factors that block retroviral replication. SAMHD1 is a triphosphohydrolase that depletes the cellular pool of dNTPs in non-cycling cells starving retroviral reverse transcription. Read More

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http://dx.doi.org/10.1099/jgv.0.001232DOI Listing
April 2019
3 Reads

Influenza virus entry via the GM3 ganglioside-mediated platelet-derived growth factor receptor β signalling pathway.

J Gen Virol 2019 Apr 14;100(4):583-601. Epub 2019 Feb 14.

1​Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium.

The possible resistance of influenza virus against existing antiviral drugs calls for new therapeutic concepts. One appealing strategy is to inhibit virus entry, in particular at the stage of internalization. This requires a better understanding of virus-host interactions during the entry process, including the role of receptor tyrosine kinases (RTKs). Read More

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http://dx.doi.org/10.1099/jgv.0.001235DOI Listing

Combined infection with Tomato yellow leaf curl virus and Rickettsia influences fecundity, attraction to infected plants and expression of immunity-related genes in the whitefly Bemisia tabaci.

J Gen Virol 2019 Apr 14;100(4):721-731. Epub 2019 Feb 14.

1​Department of Entomology, The Volcani Center, Rishon LeZion, Israel.

We have recently shown that Rickettsia, a secondary facultative bacterial symbiont that infects the whitefly B. tabaci is implicated in the transmission of Tomato yellow leaf curl virus (TYLCV). Infection with Rickettsia improved the acquisition and transmission of the virus by B. Read More

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http://dx.doi.org/10.1099/jgv.0.001233DOI Listing

Cytomegalovirus host entry and spread.

J Gen Virol 2019 Apr 7;100(4):545-553. Epub 2019 Feb 7.

School of Chemistry and Molecular Biosciences and Child Health Research Centre, University of Queensland, Brisbane, Australia.

Cytomegaloviruses (CMVs) are large, complex pathogens that persistently and systemically colonize most mammals. Human cytomegalovirus (HCMV) causes congenital harm, and has proved hard to control. One problem is that key vaccine targets - virus entry and spread in naive hosts - remain ill-defined. Read More

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http://dx.doi.org/10.1099/jgv.0.001230DOI Listing

Host protein CD63 promotes viral RNA replication by interacting with human astrovirus non-structural protein nsP1a/4.

J Gen Virol 2019 Apr 6;100(4):616-628. Epub 2019 Feb 6.

1​College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121200, Liaoning, PR China.

Human astrovirus non-structural protein nsP1a/4, located at the C-terminal end of nsP1a, is thought to be involved in regulating RNA replication. Here, we show that host protein CD63 interacts with the nsP1a protein. Further research showed that the large loop (LEL) domain of CD63 also interacts with nsP1a/4. Read More

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http://dx.doi.org/10.1099/jgv.0.001231DOI Listing
April 2019
2 Reads

Hazara nairovirus elicits differential induction of apoptosis and nucleocapsid protein cleavage in mammalian and tick cells.

J Gen Virol 2019 Mar 5;100(3):392-402. Epub 2019 Feb 5.

1​School of Molecular and Cellular Biology, University of Leeds, Leeds, LS2 9JT, UK.

The Nairoviridae family within the Bunyavirales order comprise tick-borne segmented negative-sense RNA viruses that cause serious disease in a broad range of mammals, yet cause a latent and lifelong infection in tick hosts. An important member of this family is Crimean-Congo haemorrhagic fever virus (CCHFV), which is responsible for serious human disease that results in case fatality rates of up to 30 %, and which exhibits the most geographically broad distribution of any tick-borne virus. Here, we explored differences in the cellular response of both mammalian and tick cells to nairovirus infection using Hazara virus (HAZV), which is a close relative of CCHFV within the CCHFV serogroup. Read More

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https://www.microbiologyresearch.org/content/journal/jgv/10.
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http://dx.doi.org/10.1099/jgv.0.001211DOI Listing
March 2019
3 Reads

A single cycle influenza virus coated in H7 haemagglutinin generates neutralizing antibody responses to haemagglutinin and neuraminidase glycoproteins and protection from heterotypic challenge.

J Gen Virol 2019 Mar 4;100(3):431-445. Epub 2019 Feb 4.

1​MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK.

A non-replicating form of pseudotyped influenza virus, inactivated by suppression of the haemagglutinin signal sequence (S-FLU), can act as a broadly protective vaccine. S-FLU can infect for a single round only, and induces heterotypic protection predominantly through activation of cross-reactive T cells in the lung. Unlike the licensed live attenuated virus, it cannot reassort a pandemic haemagglutinin (HA) into seasonal influenza. Read More

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http://dx.doi.org/10.1099/jgv.0.001228DOI Listing
March 2019
2 Reads

Mutagenesis of the dengue virus NS4A protein reveals a novel cytosolic N-terminal domain responsible for virus-induced cytopathic effects and intramolecular interactions within the N-terminus of NS4A.

J Gen Virol 2019 Mar 1;100(3):457-470. Epub 2019 Feb 1.

1​Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan, PR China.

The NS4A protein of dengue virus (DENV) has a cytosolic N terminus and four transmembrane domains. NS4A participates in RNA replication and the host antiviral response. However, the roles of amino acid residues within the N-terminus of NS4A during the life cycle of DENV are not clear. Read More

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http://dx.doi.org/10.1099/jgv.0.001227DOI Listing

Hepatitis E virus polymerase binds to IFIT1 to protect the viral RNA from IFIT1-mediated translation inhibition.

J Gen Virol 2019 Mar 31;100(3):471-483. Epub 2019 Jan 31.

1​Agharkar Research Institute, Nanobioscience Group, G. G. Agarkar Road, Pune 411004, India.

Hepatitis E virus (HEV) induces interferons and regulates the induction of interferon-stimulated genes (ISGs) in the host cell. HEV infection has been shown to promote the expression of different ISGs, such as ISG15, IFIT1, MX1, RSAD2/Viperin and CxCL10, in cell culture and animal models. Interferon-induced protein with tetratricopeptide repeat 1 (IFIT1) is an ISG-encoded protein that inhibits the translation of viral RNA, having 5'-triphosphate or the mRNA lacking 2'-O-methylation on the 5'cap. Read More

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http://www.microbiologyresearch.org/content/journal/jgv/10.1
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http://dx.doi.org/10.1099/jgv.0.001229DOI Listing
March 2019
8 Reads

Effect of foot-and-mouth disease virus 3C protease B2 β-strand proline mutagenesis on expression and processing of the P1 polypeptide using a plasmid expression vector.

J Gen Virol 2019 Mar 31;100(3):446-456. Epub 2019 Jan 31.

3​U. S. Department of Homeland Security Science and Technology Directorate, Plum Island Animal Disease Center, Greenport, NY 11944, USA.

The production of experimental molecular vaccines against foot-and-mouth disease virus utilizes the viral encoded 3C protease for processing of the P1 polyprotein. Expression of wild type 3C protease is detrimental to host cells. The molecular vaccine constructs containing the 3C protease L127P mutant significantly reduce adverse effects associated with protease expression while retaining the ability to process and assemble virus-like particles. Read More

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http://dx.doi.org/10.1099/jgv.0.001204DOI Listing
March 2019
1 Read

Vehicles of intercellular communication: exosomes and HIV-1.

J Gen Virol 2019 Mar 31;100(3):350-366. Epub 2019 Jan 31.

4​Department of Pharmacologic Sciences, Basic Sciences Tower, Rm 8-142, Stony Brook, University School of Medicine, Stony Brook, NY 11794-8651, USA.

The terms extracellular vesicles, microvesicles, oncosomes, or exosomes are often used interchangeably as descriptors of particles that are released from cells and comprise a lipid membrane that encapsulates nucleic acids and proteins. Although these entities are defined based on a specific size range and/or mechanism of release, the terminology is often ambiguous. Nevertheless, these vesicles are increasingly recognized as important modulators of intercellular communication. Read More

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http://dx.doi.org/10.1099/jgv.0.001193DOI Listing
March 2019
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Characterization of ssDNA aptamers specifically directed against Trachinotus ovatus NNV (GTONNV)-infected cells with antiviral activities.

J Gen Virol 2019 Mar 4;100(3):380-391. Epub 2019 Feb 4.

1​Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning, PR China.

Nervous necrosis virus (NNV), is one of the most fatal viruses in marine fish aquaculture, and is capable of infecting over 50 different fish species. Trachinotus ovatus NNV (GTONNV) was isolated from diseased golden pompano. This T. Read More

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http://dx.doi.org/10.1099/jgv.0.001226DOI Listing
March 2019
2 Reads

The baculovirus Ac108 protein is a per os infectivity factor and a component of the ODV entry complex.

J Gen Virol 2019 Apr 29;100(4):669-678. Epub 2019 Jan 29.

Laboratory of Virology, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Wild-type ODVs (Wt) have an intact ODV entry complex in their envelope and are orally infectious towards insect larvae (left panel). In the absence of Ac108 (mut ac108), the stable core is still present but nevertheless fails to form an entry complex, affecting the ODV oral infectivity (right panel). The components of the core complex are depicted in yellow and the loosely associated components are depicted in red. Read More

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http://dx.doi.org/10.1099/jgv.0.001200DOI Listing
April 2019
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Transmembrane regions of bovine herpesvirus 1-encoded UL49.5 and glycoprotein M regulate complex maturation and ER-Golgi trafficking.

J Gen Virol 2019 Mar 29;100(3):497-510. Epub 2019 Jan 29.

1​Laboratory of Virus Molecular Biology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.

Bovine herpesvirus 1 (BoHV-1)-encoded UL49.5 (a homologue of herpesvirus glycoprotein N) can combine different functions, regulated by complex formation with viral glycoprotein M (gM). We aimed to identify the mechanisms governing the immunomodulatory activity of BoHV-1 UL49. Read More

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http://dx.doi.org/10.1099/jgv.0.001224DOI Listing
March 2019
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Identification of a novel membrane-associated protein from the S7 segment of grass carp reovirus.

J Gen Virol 2019 Mar 28;100(3):369-379. Epub 2019 Jan 28.

1​National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, PR China.

Aquareovirus is a genus of viruses in family Reoviridae, subfamily Spinareovirinae, members of which infect fish, shellfish and crustaceans. Grass carp reovirus (GCRV), a genotype 1 reovirus isolated from grass carp, has served as a model strain for investigating aquareovirus-host interactions. Herein, we report a neglected open reading frame (ORF), tentatively named NS12, residing between NS16 and NS31 in segment 7 (S7) of the virus genome. Read More

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http://dx.doi.org/10.1099/jgv.0.001223DOI Listing

Characterization of Teviot virus, an Australian bat-borne paramyxovirus.

J Gen Virol 2019 Mar 28;100(3):403-413. Epub 2019 Jan 28.

1​CSIRO Health and Biosecurity, Australian Animal Health Laboratory, Geelong, Australia.

Bats are the reservoir hosts for multiple viruses with zoonotic potential, including coronaviruses, paramyxoviruses and filoviruses. Urine collected from Australian pteropid bats was assessed for the presence of paramyxoviruses. One of the viruses isolated was Teviot virus (TevPV), a novel rubulavirus previously isolated from pteropid bat urine throughout the east coast of Australia. Read More

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https://www.microbiologyresearch.org/content/journal/jgv/10.
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http://dx.doi.org/10.1099/jgv.0.001214DOI Listing
March 2019
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Kodoja: A workflow for virus detection in plants using k-mer analysis of RNA-sequencing data.

J Gen Virol 2019 Mar 24;100(3):533-542. Epub 2019 Jan 24.

2​Information and Computational Sciences Group, The James Hutton Institute, Dundee, DD2 5DA, UK.

RNA-sequencing of plant material allows for hypothesis-free detection of multiple viruses simultaneously. This methodology relies on bioinformatics workflows for virus identification. Most workflows are designed for human clinical data, and few go beyond sequence mapping for virus identification. Read More

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http://dx.doi.org/10.1099/jgv.0.001210DOI Listing
March 2019
3 Reads

Oncogenicitiy Comparison of Human Papillomavirus Type 52 E6 Variants.

J Gen Virol 2019 Mar 24;100(3):484-496. Epub 2019 Jan 24.

1​Department of Microbiology, Faulty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR.

Human papillomavirus (HPV) infection contributes to virtually all cases of cervical cancer, the fourth most common cancer affecting women worldwide. The oncogenicity of HPV is mainly attributable to the E6 and E7 oncoproteins. HPV-52 is the seventh most common HPV type globally, but it has a remarkably high prevalence in East Asia. Read More

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http://dx.doi.org/10.1099/jgv.0.001222DOI Listing
March 2019
4 Reads

Increased predominance of HIV-1 CRF01_AE and its recombinants in the Philippines.

J Gen Virol 2019 Mar 24;100(3):511-522. Epub 2019 Jan 24.

1​Department of Medicine, Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA.

The growth rate of new HIV infections in the Philippines was the fastest of any countries in the Asia-Pacific region between 2010 and 2016. To date, HIV-1 subtyping results in the Philippines have been determined by characterizing only partial viral genome sequences. It is not known whether recombination occurs in the majority of unsequenced genome regions. Read More

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http://dx.doi.org/10.1099/jgv.0.001198DOI Listing
March 2019
2 Reads

ICTV Virus Taxonomy Profile: Parvoviridae.

J Gen Virol 2019 Mar 23;100(3):367-368. Epub 2019 Jan 23.

12​Centre de Recherche de Microbiologie et Biotechnologie, INRS-Institut Armand-Frappier Laval, QC H7V 1B7, Canada.

Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, single-stranded DNA genomes of 4-6 kb. Viruses in two subfamilies, the Parvovirinae and Densovirinae, are distinguished primarily by their respective ability to infect vertebrates (including humans) versus invertebrates. Being genetically limited, most parvoviruses require actively dividing host cells and are host and/or tissue specific. Read More

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http://dx.doi.org/10.1099/jgv.0.001212DOI Listing
March 2019
1 Read
3.183 Impact Factor

The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NFκB-dependent promoter antagonism in chicken cells.

J Gen Virol 2019 Mar 23;100(3):414-430. Epub 2019 Jan 23.

1​The Pirbright Institute, Pirbright, Woking, UK.

The accessory protein, PB1-F2, of influenza A virus (IAV) functions in a chicken host to prolong infectious virus shedding and thus the transmission window. Here we show that this delay in virus clearance by PB1-F2 in chickens is accompanied by reduced transcript levels of type 1 interferon (IFN)-induced genes and NFκB-activated pro-inflammation cytokines. In vitro, two avian influenza isolate-derived PB1-F2 proteins, H9N2 UDL01 and H5N1 5092, exhibited the same antagonism of the IFN and pro-inflammation induction pathways seen in vivo, but to different extents. Read More

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http://dx.doi.org/10.1099/jgv.0.001220DOI Listing
March 2019
2 Reads

Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus - an unusual member of a putative new genus within the Potyviridae.

J Gen Virol 2019 Feb 22;100(2):308-320. Epub 2019 Jan 22.

1​Department Phytomedicine, Leibniz University Hannover, Institute of Horticultural Production Systems, Herrenhäuser Str. 2, 30419, Hannover, Germany.

Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5' untranslated region (UTR) of 13 nt and a 3' UTR consisting of 586 nt that is not polyadenylated. Read More

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http://dx.doi.org/10.1099/jgv.0.001207DOI Listing
February 2019
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Impact of naturally occurring variation in the human papillomavirus 52 capsid proteins on recognition by type-specific neutralising antibodies.

J Gen Virol 2019 Feb 18;100(2):237-245. Epub 2019 Jan 18.

1​Virus Reference Department, Public Health England, London, UK.

We investigated the impact of naturally occurring variation within the major (L1) and minor (L2) capsid proteins on the antigenicity of human papillomavirus (HPV) type 52 (HPV52). L1L2 pseudoviruses (PsVs) representing HPV52 lineage and sublineage variants A1, A2, B1, B2, C and D were created and tested against serum from naturally infected individuals, preclinical antisera raised against HPV52 A1 and D virus-like particles (VLPs) and neutralising monoclonal antibodies (MAbs) raised against HPV52 A1 VLP. HPV52 lineage D PsV displayed a median 3. Read More

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http://dx.doi.org/10.1099/jgv.0.001213DOI Listing
February 2019
5 Reads

Analyses of four new Caulobacter Phicbkviruses indicate independent lineages.

J Gen Virol 2019 Feb 18;100(2):321-331. Epub 2019 Jan 18.

Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.

Bacteriophages with genomes larger than 200 kbp are considered giant phages, and the giant Phicbkviruses are the most frequently isolated Caulobacter crescentus phages. In this study, we compare six bacteriophage genomes that differ from the genomes of the majority of Phicbkviruses. Four of these genomes are much larger than those of the rest of the Phicbkviruses, with genome sizes that are more than 250 kbp. Read More

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http://dx.doi.org/10.1099/jgv.0.001218DOI Listing
February 2019
3 Reads