1,589 results match your criteria Journal of Gene Medicine [Journal]


Non-viral gene delivery for cancer immunotherapy.

J Gene Med 2019 Apr 16:e3092. Epub 2019 Apr 16.

State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

The past decades have witnessed the revolutionizing development in cancer immunotherapies, which boost cancer-specific immune responses for the long-term tumor regression. However, immunotherapy still has limitations, including off-target side effects, long processing times, and limited patient responses. These disadvantages of current immunotherapy are being addressed by improving our understanding of the immune system, as well as by establishing combinational approaches. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/jgm.3092
Publisher Site
http://dx.doi.org/10.1002/jgm.3092DOI Listing
April 2019
1 Read

Non-viral nucleic acid delivery to central nervous system and brain tumor.

J Gene Med 2019 Apr 12:e3091. Epub 2019 Apr 12.

Department of Pharmacy, Zhongshan Hospital, and School of Pharmacy, Fudan University, Shanghai, China.

Gene therapy is a rapidly emerging remedial route for many serious incurable diseases, such as central nervous system (CNS) diseases. Currently, nucleic acid medicines including DNAs encoding therapeutic or destructive proteins, siRNAs or miRNAs, have been successfully delivered to the CNS with gene delivery vectors via various routes of administration and subsequently exhibited remarkable therapeutic efficiency. Among these vectors, non-viral vectors are favorable for delivering genes into CNS due to many special characteristics such as low toxicity and preexisting immunogenicity, high gene loading efficiency and easy surface modification. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3091DOI Listing

Virus inspired and mimetic designs in nonviral gene delivery.

J Gene Med 2019 Apr 10:e3090. Epub 2019 Apr 10.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, P. R. China.

Virus-inspired mimics for nucleic acid transportation have drawn intensive attentions in the past decade, especially the derivative microenvironment stimuli responsive designs. In the present mini-review, the smart designs of gene carriers for overcoming biological barriers and realizing efficient delivery have been categorized by different "triggers" provided by tumor cells, including pH, redox potentials, adenosine triphosphate (ATP), enzymes and reactive oxygen species (ROS). Some dual/multi-responsive gene vectors have also been introduced that showed more precise and efficient delivery in the complicated environment of human body. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/jgm.3090
Publisher Site
http://dx.doi.org/10.1002/jgm.3090DOI Listing
April 2019
5 Reads

Transcutaneous Delivery of DNA/mRNA for Cancer Therapeutic Vaccination.

J Gene Med 2019 Apr 8:e3089. Epub 2019 Apr 8.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Therapeutic vaccination is a promising strategy in immunotherapy of cancers. It eradicates cancer cells by evoking and strengthening patients' own immune system. Because of the easy access and sophisticated immune networks, the skin becomes an ideal target organ for vaccination. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3089DOI Listing

Tumor microenvironment as the "regulator" and "target" for gene therapy.

J Gene Med 2019 Apr 2:e3088. Epub 2019 Apr 2.

Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.

Aims: In this review, we focus on strategies of designing functional nano gene carriers as well as choosing therapeutic genes targeting on tumor microenvironment.

Discussion: Gene mutations have a great impact on the occurrence of cancer, thus gene therapy plays a major role in cancer therapy and has the promise to cure cancer. Well-designed gene therapy largely relies on effective gene carriers, which can be divided into viral carriers and non-viral carriers. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/jgm.3088
Publisher Site
http://dx.doi.org/10.1002/jgm.3088DOI Listing
April 2019
5 Reads

Improved biosafety of a lentiviral vector by reducing cellular gene activation.

J Gene Med 2019 Mar 22:e3087. Epub 2019 Mar 22.

Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Introduction: Lentiviral vectors (LVs) have enhancer activity and/or transcriptional read-through (EATRT) properties that can lead to the activation of adjacent genes. Consequently, patients may be at increased risk for adverse effects if such vectors are used clinically.

Aim: We, therefore, assessed the abilities of different "pro-LV"-like constructs on decreasing its EATRT, including the "pro-LV" vector bearing a chimeric ΔLTR of human foamy virus R-U5 region replaced with that of an LV (HF). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3087DOI Listing
March 2019
1 Read

MDM2 and its functional polymorphism SNP309 contribute to the development of esophageal carcinoma.

J Gene Med 2019 Mar 12:e3086. Epub 2019 Mar 12.

Henan Key Laboratory for Esophageal Cancer Research, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Background: Overexpression of the murine double minute 2 (MDM2) has been explored in many tumors with high proliferation and anti-apoptosis ability. However, the role of MDM2 and its functional single nucleotide polymorphism (SNP) rs2279744 (also known as SNP309) in esophageal squamous cell carcinoma (ESCC) remains unclear.

Methods: We performed a genotype study of blood samples in 360 ESCC patients and 360 healthy control individuals to determine the risk of various rs2279744 in ESCC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3086DOI Listing

Long non-coding RNA MIR22HG inhibits cell proliferation and migration in cholangiocarcinoma by negatively regulating the Wnt/β-catenin signaling pathway.

J Gene Med 2019 Mar 11:e3085. Epub 2019 Mar 11.

Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, Hunan, China.

Background: Cholangiocarcinoma (CCA) is one of the most common primary biliary malignant tumors with a high mortality. MIR22HG has been reported to act as a tumor-suppressor gene in several types of cancers. However, the role and molecular regulatory mechanism of MIR22HG in CCA still remains unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3085DOI Listing
March 2019
1 Read

Organic/inorganic nanohybrids as multifunctional gene delivery systems.

J Gene Med 2019 Mar 8:e3084. Epub 2019 Mar 8.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China.

In this review, we summarize the rational design and versatile application of organic/inorganic hybrid gene carriers as multifunctional delivery systems. Organic/inorganic nanohybrids with both organic and inorganic components in one nanoparticle have attracted intense attention because of their favorable properties. Particularly, nanohybrids comprising cationic polymers and inorganic nanoparticles are considered to be promising candidates as multifunctional gene delivery systems. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3084DOI Listing
March 2019
17 Reads

Long non-coding RNA TTN-AS1 facilitates tumorigenesis of papillary thyroid cancer through modulating the miR-153-3p/ZNRF2 axis.

J Gene Med 2019 Feb 27:e3083. Epub 2019 Feb 27.

Department of Ultrasound, the Second Affiliated Hospital, School of Medical, Zhejiang University, Hangzhou, Zhejiang, China.

Background: Long non-coding RNAs (lncRNAs) are crucial modulators in the tumorigenesis of numerous cancers, including papillary thyroid cancer (PTC). However, it is unclear whether lncRNA TTN antisense RNA 1 (TTN-AS1) can regulate PTC progression. The present study aimed to reveal the mechanism and function of TTN-AS1 in PTC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3083DOI Listing
February 2019
1 Read

Creating cell and animal models of human disease by genome editing using CRISPR/Cas9.

J Gene Med 2019 Apr 15;21(4):e3082. Epub 2019 Mar 15.

Department of Genetics, Shiraz University of Medical Science, Shiraz, Iran.

A set of unique sequences in bacterial genomes, responsible for protecting bacteria against bacteriophages, has recently been used for the genetic manipulation of specific points in the genome. These systems consist of one RNA component and one enzyme component, known as CRISPR ("clustered regularly interspaced short palindromic repeats") and Cas9, respectively. The present review focuses on the applications of CRISPR/Cas9 technology in the development of cellular and animal models of human disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3082DOI Listing
April 2019
2 Reads
2.472 Impact Factor

Genetic polymorphism of methylenetetrahydrofolate reductase is associated with insulin resistance in Egyptian women with polycystic ovary syndrome.

J Gene Med 2019 Apr 4;21(4):e3076. Epub 2019 Mar 4.

Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt.

Background: A common polymorphism (677C to T; Ala to Val) in the methylenetetrahydrofolate reductase (MTHFR) gene is associated with decreased specific MTHFR activity and elevation of homocysteine. The present study aimed to investigate the association between a single nucleotide polymorphism (SNP) in the MTHFR 677C>T gene and insulin resistance in women with polycystic ovary syndrome (PCOS).

Methods: Two-hundred patients with PCOS were included in this case-control study: 100 patients with insulin resistance and 100 patients without insulin resistance were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3076DOI Listing
April 2019
1 Read

Identification of microRNA expression in sentinel lymph nodes from patients with breast cancer via RNA sequencing for diagnostic accuracy.

J Gene Med 2019 Apr 3;21(4):e3075. Epub 2019 Mar 3.

Department of Ultrasonography, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Background: Sentinel lymph node (SLN) property assessment (with or without metastasis) is important when deciding the surgery for breast cancer; however, the current diagnosis of SLN metastasis remains to be studied. microRNAs (miRNAs) have been confirmed previously as a molecular marker for the diagnosis, development and prognosis of tumors. However, the detailed role of miRNAs in the diagnosis of SLN metastasis has not been reported. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3075DOI Listing
April 2019
1 Read
2.472 Impact Factor

Long non-coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR-4425/MTA3 axis.

J Gene Med 2019 Apr 27;21(4):e3074. Epub 2019 Feb 27.

Department of Neurosurgery, Shanghai Fourth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Glioma is a type of malignant tumor that occurs in the central nervous system of adults. Long non-coding RNAs (lncRNAs) that potentially participate in the initiation and progression of glioma have been widely reported. As a now-found lncRNA, HLA complex group 11 (HCG11) has not yet been studied in glioma. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3074DOI Listing
April 2019
1 Read

Whole exome sequencing identified a novel mutation (p.Ala1884Pro) of β-spectrin in a Chinese family with hereditary spherocytosis.

J Gene Med 2019 Feb 11;21(2-3):e3073. Epub 2019 Feb 11.

Department of Cell Biology, The School of Life Sciences, Central South University, Changsha, China.

Background: Hereditary spherocytosis (HS) is an inherited disorder of erythrocyte. The typical feature of HS is the presence of spherical-shaped erythrocytes on the peripheral blood smear. According to previous studies, more than five candidate genes, such as ANK1, SPTB, SPTA1, SLC4A1 and EPB42 have been identified in HS patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3073DOI Listing
February 2019
4 Reads

Ankyrin repeat domain 1: A novel gene for cardiac septal defects.

J Gene Med 2019 Apr 19;21(4):e3070. Epub 2019 Feb 19.

Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.

Introduction: Cardiac septal defects account for more than 50% of congenital heart defects. Ankyrin repeat domain 1 (ANKRD1) is an important transcription factor that is mutated in multiple cardiac diseases; however, a relationship between the ANKRD1 mutation and cardiac septal defects has not been described.

Methods: We examined genetic mutations in a large family with three cardiac septal defect patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3070DOI Listing
April 2019
2 Reads

Association between the LIPG polymorphisms and serum lipid levels in the Maonan and Han populations.

J Gene Med 2019 Feb 4;21(2-3):e3071. Epub 2019 Feb 4.

Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, China.

Introduction: The Maonan population is a relatively isolated minority in China. Little is known about endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations. The present study aimed to detect the association of several LIPG SNPs and environmental factors with serum lipid levels in the Chinese Maonan and Han populations. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3071
Publisher Site
http://dx.doi.org/10.1002/jgm.3071DOI Listing
February 2019
12 Reads

Adenoviral delivery of adiponectin ameliorates osteogenesis around implants in ovariectomized rats.

J Gene Med 2019 Feb 22;21(2-3):e3069. Epub 2019 Jan 22.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Background: Adiponectin (APN) has been reported to promote bone formation. However, it is difficult to utilize a conventional method that administers sufficient APN to the implant site. The present study investigated the efficacy of an APN transgene to accelerate the implant osseointegration in ovariectomized (OVX) rats. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3069
Publisher Site
http://dx.doi.org/10.1002/jgm.3069DOI Listing
February 2019
3 Reads

Development of a liver-specific Tet-off AAV8 vector for improved safety of insulin gene therapy for diabetes.

J Gene Med 2019 Jan 20;21(1):e3067. Epub 2019 Jan 20.

Department of Medicine, National University of Singapore, Singapore.

Background: Diabetes mellitus is caused by a partial or complete lack of insulin production in the body. We have previously shown that a single injection of an adeno-associated virus serotype 8 (AAV8) vector carrying a modified and codon optimized human insulin gene induced hepatic production of insulin and corrected streptozotocin (STZ)-induced diabetes in mice for more than 1 year. Insulin production was constitutive, analogous to long-acting insulin therapy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3067DOI Listing
January 2019
2 Reads

Corrigendum: Polymer-coated viral vectors: hybrid nanosystems for gene therapy.

Authors:

J Gene Med 2019 Jan;21(1):e3068

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3068DOI Listing
January 2019
2 Reads

P2RY12:rs7637803 TT variant genotype increases coronary artery aneurysm risk in Kawasaki disease in a southern Chinese population.

J Gene Med 2019 Jan 10;21(1):e3066. Epub 2019 Jan 10.

Department of Clinical Biological Resource Bank, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Background: Activated-platelet increases the risk of thrombosis in Kawasaki disease (KD) patients with a coronary artery aneurysm (CAA). The ADP pathway is one of the platelet activation and aggregation pathways. The P2RY12 gene encodes the ADP receptor that is highly concentrated on platelets. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3066
Publisher Site
http://dx.doi.org/10.1002/jgm.3066DOI Listing
January 2019
5 Reads
2.472 Impact Factor

Long non-coding RNAs in cancer: Another layer of complexity.

J Gene Med 2019 Jan 8;21(1):e3065. Epub 2019 Jan 8.

Department of Genetics, Federal University of Parana, Curitiba, Brazil.

We review the most well characterized long non-coding RNAs (lncRNAs) with important roles in hallmarks of cancer, additionally including lncRNAs with a higher potential for clinical application. LncRNAs are transcripts larger than 200 nucleotides in length that do not appear to have protein-coding potential, although some of those may produce small functional peptides. These transcripts have attracted significant attention from researchers as a result of their role in genetic regulation, including epigenetic, transcriptional and post-transcriptional regulation, being involved in numerous biological processes, as well as being associated with multifactorial diseases, including tumorigenesis. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/jgm.3065
Publisher Site
http://dx.doi.org/10.1002/jgm.3065DOI Listing
January 2019
38 Reads

Cloning of carrier cells infected with oncolytic adenovirus driven by midkine promoter and biosafety studies.

J Gene Med 2019 Feb 1;21(2-3):e3064. Epub 2019 Feb 1.

Department of Obstetrics and Gynecology, School of Medicine, Ehime University, Ehime, Japan.

Background: A549 carrier cells infected with oncolytic adenovirus can induce complete tumor reduction of subcutaneous ovarian tumors but not intraperitoneal disseminated ovarian tumors. This appears to be a result of the insufficient antitumor effect of A549 carrier cells. Therefore, in the present study, we cloned a novel carrier cell with the aim of improving the antitumor effects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3064DOI Listing
February 2019
11 Reads

TP53 somatic mutations are associated with somatic mitogenome substitutions but not indels in colorectal cancer cells.

J Gene Med 2019 Jan 7;21(1):e3063. Epub 2019 Jan 7.

Division of Molecular and Forensic Genetics, Department of Forensic Medicine, Faculty of Medicine, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.

Background: p53 is a tumour suppressor protein that is involved in many cancer-related processes. Growing evidence suggests that p53 also plays an important role in mitochondrial (mtDNA) maintenance. Somatic mitogenome mutations are frequently observed in colorectal cancer (CC) cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3063DOI Listing
January 2019
2 Reads

HIF-1α promotes bone marrow stromal cell migration to the injury site and enhances functional recovery after spinal cord injury in rats.

J Gene Med 2018 Dec 11;20(12):e3062. Epub 2018 Dec 11.

Department of Spine Surgery, Beijing Jishuitan Hospital, Beijing, China.

Background: Spinal cord injury (SCI) is a severe health problem worldwide, and efficacious strategies to properly repair SCI have not yet been developed. Recently, gene and cell therapies as alternative treatments for SCI have been proposed to comprise safe and promising strategies.

Methods: The present study investigated the therapeutic effects and underlying mechanisms of hypoxia-inducible factor-1α carried in recombinant adenovirus (Adv-HIF-1α), as administered immediately after SCI in adult rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3062DOI Listing
December 2018
4 Reads

Telomere length, ZNF208 genetic variants and risk of chronic obstructive pulmonary disease in the Hainan Li population.

J Gene Med 2018 Dec 28;20(12):e3061. Epub 2018 Nov 28.

Department of General Practice, Hainan General Hospital, Haikou, Hainan, China.

Background: Chronic obstructive pulmonary disease (COPD) is a disease characterized by airflow limitation. It is not completely reversible and shows progressive development. ZNF208 rs8105767 affects telomere length, although the impact of telomere on COPD is still controversial. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3061
Publisher Site
http://dx.doi.org/10.1002/jgm.3061DOI Listing
December 2018
6 Reads

Enhancing atrial-specific gene expression using a calsequestrin cis-regulatory module 4 with a sarcolipin promoter.

J Gene Med 2018 Dec 4;20(12):e3060. Epub 2018 Dec 4.

Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Background: Cardiac gene therapy using the adeno-associated virus serotype 9 vector is widely used because of its efficient transduction. However, the promoters used to drive expression often cause off-target localization. To overcome this, studies have applied cardiac-specific promoters, although expression is debilitated compared to that of ubiquitous promoters. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3060DOI Listing
December 2018
15 Reads
2.472 Impact Factor

Discordance in TLR2 (-196 to -174) polymorphism effect on HIV infection risk.

J Gene Med 2018 Oct 12;20(10-11):e3051. Epub 2018 Oct 12.

Surgery, Biochemistry and Immunology Department, School of Medicine, University of Malaga, Málaga, Spain.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3051
Publisher Site
http://dx.doi.org/10.1002/jgm.3051DOI Listing
October 2018
12 Reads

IL1R1 and IL1R2 polymorphisms were associated with tuberculosis risk: A pilot study.

J Gene Med 2018 Oct 18;20(10-11):e3057. Epub 2018 Oct 18.

Department of Respiratory and critical diseases medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Background: Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family. However, few studies have reported on the polymorphisms of IL1R1 and IL1R2 in TB patients. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3057
Publisher Site
http://dx.doi.org/10.1002/jgm.3057DOI Listing
October 2018
18 Reads

Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer.

J Gene Med 2018 Dec 8;20(12):e3056. Epub 2018 Nov 8.

Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.

Background: DPP8 is a member of the dipeptidyl peptidase IV family, which belongs to the S9b protease subfamily. It regulates cell proliferation, apoptosis, migration and invasion during cancer progression.

Methods: To investigate the role of DPP8 in cervical cancer, we examined DPP8 levels in cervical cancer tissues and cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3056DOI Listing
December 2018
4 Reads
2.470 Impact Factor

Association between PTCH1 and RAD54B single-nucleotide polymorphisms and non-syndromic orofacial clefts in a northern Chinese population.

J Gene Med 2018 Dec 5;20(12):e3055. Epub 2018 Nov 5.

Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, Heilongjiang, China.

Background: Non-syndromic orofacial clefts (NSOC) is one of the most common congenital malformations, and its etiology involves both genetic and environmental factors. The present aimed to investigate the association of six single nucleotide polymorphisms (SNPs) (rs10512248 in PTCH1, rs12681366 and rs958447 in RAD54B, rs13317 in FGFR1, rs1838105 and rs4968247 in WNT9B) with NSOC in a Northern Chinese population.

Methods: In the present study, HI-SNP technology was used to conduct genotyping of the six SNPs (rs10512248, rs12681366, rs957448, rs13317, rs1838105 and rs4968247) in 596 patients with NSOC and 466 healthy individuals from a Northern Chinese population. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3055DOI Listing
December 2018
3 Reads

Arg399Gln substitution in XRCC1 as a prognostic and predictive biomarker for prostate cancer: Evidence from 8662 subjects and a structural analysis.

J Gene Med 2018 Oct 26;20(10-11):e3053. Epub 2018 Sep 26.

Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.

Background: The Arg399Gln polymorphism in the X-ray repair cross-complementing group 1 gene (XRCC1) may alter the risk of prostate cancer (PCa). The present study aimed to investigate the association of the XRCC1-Arg399Gln polymorphism with PCa risk in an Iranian population, as followed by a meta-analysis and an in silico analysis.

Methods: In a case-control study, 360 subjects were included (180 men with PCa and 180 healthy controls). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3053DOI Listing
October 2018
22 Reads

The association between polymorphism of the BDNF gene and cigarette smoking in the Iranian population.

J Gene Med 2018 Oct 12;20(10-11):e3052. Epub 2018 Sep 12.

Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Background: Cigarette smoking remains a serious public health problem. Environmental and genetic factors both play critical roles in the process of cigarette smoking. We aimed to investigate the associations between single nucleotide polymorphisms (SNP) in the BDNF gene and cigarette smoking in the Iranian population. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3052DOI Listing
October 2018
6 Reads

Minimal conditioning in Fanconi anemia promotes multi-lineage marrow engraftment at 10-fold lower cell doses.

J Gene Med 2018 Oct 1;20(10-11):e3050. Epub 2018 Oct 1.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Background: Gene therapy approaches for the treatment of Fanconi anemia (FA) hold promise for patients without a suitably matched donor for an allogeneic bone marrow transplant. However, significant limitations include the collection of sufficient stem cell numbers from patients, the fragility of these cells during ex vivo manipulation, and clinically meaningful engraftment following transplantation. With these challenges in mind, we were interested in determining (i) whether gene-corrected cells at progressively lower numbers can successfully engraft in FA; (ii) whether low-dose conditioning facilitates this engraftment; and (iii) whether these cells can be selected for post-transplant. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3050
Publisher Site
http://dx.doi.org/10.1002/jgm.3050DOI Listing
October 2018
18 Reads

Polymorphisms in TH1-TH2 cytokine and receptor genes associated with risk of vertical HIV transmission, in Mumbai, India.

J Gene Med 2018 Oct 4;20(10-11):e3047. Epub 2018 Sep 4.

Department of Infectious Diseases Biology, National Institute for Research in Reproductive Health (DHR/ICMR), Mumbai, Maharashtra, India.

Background: Vertical HIV transmission does not occur in all exposed infants. Many infants remain HIV uninfected even after exposure. This is partly attributed to the host genes involving cytokine production, which is rarely documented in vertical transmission. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3047DOI Listing
October 2018
25 Reads

Neuraminidase-mediated desialylation augments AAV9-mediated gene expression in skeletal muscle.

J Gene Med 2018 Sep 4;20(9):e3049. Epub 2018 Sep 4.

Department of Medicine, Division of Cardiovascular Medicine, University of Virginia, Charlottesville, VA, USA.

Background: Following systemic delivery, AAV9-mediated gene expression is significantly increased in ischemic versus non-ischemic muscle, suggesting that AAV9 is an attractive vector for treating peripheral arterial disease. Potential mechanisms underlying ischemia-augmented expression include: (i) increased vascular permeability and (ii) "unmasking" of endogenous AAV9 receptors. In the present study, we aimed to reconstitute the ischemic induction of AAV9 in vivo, using local injection of histamine (to increase vascular permeability) and neuraminidase (to desialylate cell surface glycans). Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3049
Publisher Site
http://dx.doi.org/10.1002/jgm.3049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209320PMC
September 2018
56 Reads

Association of genetic polymorphisms in VEGF -460, -7 and -583 and hematocrit level with the development of idiopathic recurrent pregnancy loss and a meta-analysis.

J Gene Med 2018 Sep 30;20(9):e3048. Epub 2018 Aug 30.

Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.

Background: The present study was performed to investigate whether genetic variants of VEGF are associated with recurrent pregnancy loss (RPL) in Korean women and to provide insight into the role of VEGF in the pathogenesis of RPL development.

Methods: A cohort of 384 women with idiopathic RPL with a history of two or more uxexplained consecutive early pregnancy losses and 236 control women were recruited from an infertility center of university-teaching hospital in Korea between March 1999 and February 2010. We examined three VEGF polymorphisms (rs833061, rs3025020 and rs25648). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3048DOI Listing
September 2018
7 Reads

Transduction efficacy and retrograde movement of a lentiviral vector pseudotyped by modified rabies glycoprotein throughout the trisynaptic circuit of the rat hippocampus.

J Gene Med 2018 Sep 15;20(9):e3046. Epub 2018 Aug 15.

Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.

Background: The trisynaptic circuit (entorhinal cortex-dentate gyrus-CA3-CA1) is a key unidirectional network in the hippocampus. Damage to the hippocampus interrupts this circuit and causes neurological disorders. Efficient delivery of therapeutic genes into this network is of great interest with respect to treating trisynaptic circuit pathologies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3046DOI Listing
September 2018
5 Reads

MIF-173G/C (rs755622) polymorphism as a risk factor for acute lymphoblastic leukemia development in children.

J Gene Med 2018 Sep 2;20(9):e3044. Epub 2018 Aug 2.

Department of Biochemistry, Faculty of Sciences, Damietta University, New Damietta, Egypt.

Background: Macrophage inhibitory factor (MIF) is a pro-inflammatory cytokine modulating monocyte motility and a pleiotropic regulator of different biological and cellular processes. The MIF-173G/C (rs755622) polymorphism is found in the promoter region and affects its activity. The present study investigated the MIF polymorphism as a risk factor for the development of acute lymphoblastic leukemia (ALL) in Egyptian children. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3044DOI Listing
September 2018
8 Reads

Thymosin β4 suppresses CCl -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.

J Gene Med 2018 Sep 15;20(9):e3043. Epub 2018 Aug 15.

Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

Background: The present study aimed to clarify the effects of thymosin β4 (Tβ4) on CCl -induced hepatic fibrosis in mice and to further explore the underlying mechanisms.

Methods: Expression of Tβ4 in fibrotic liver tissues was assessed by a quantitative real time-reverse transcriptase polymerase chain reaction and immunohistochemistry. The effects of intraperitoneal adeno-associated virus-Tβ4 (AAV-Tβ4) on CCl -induced hepatic fibrosis were observed by the evaluation of collagen deposition, hepatic stellate cell (HSC) activation and pro-fibrotic cytokine expression. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3043DOI Listing
September 2018
8 Reads

Bone morphogenetic protein-2 is a stronger inducer of osteogenesis within muscle tissue than heterodimeric bone morphogenetic protein-2/6 and -2/7: Implications for expedited gene-enhanced bone repair.

J Gene Med 2018 Sep 24;20(9):e3042. Epub 2018 Jul 24.

Department of Orthopedic Surgery, Physical Medicine and Rehabilitation, University Hospital Grosshadern, Ludwig-Maximilians-University Munich, Munich, Germany.

Background: Bone morphogenetic protein (BMP)-2 gene-activated muscle tissue fragments can regenerate large bone defects in preclinical animal models. The use of tissue fragments instead of isolated cells expedites gene-enhanced tissue engineering and may increase the possibility of clinical translation. The present in vitro study investigated whether the osteoinductive effect of BMP-2 on muscle tissue fragments can be enhanced using the heterodimers BMP-2/6 or BMP-2/7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3042DOI Listing
September 2018
14 Reads

Epidermal growth factor receptor targeted methotrexate and small interfering RNA co-delivery.

J Gene Med 2018 Jul 17;20(7-8):e3041. Epub 2018 Jul 17.

Pharmaceutical Biotechnology, Department of Pharmacy, Ludwig-Maximilians-Universität München, Munich, Germany.

Background: Developing new drug delivery carriers addressing chemoresistance is still full of challenges and opportunities. As the rapid development of small interfering RNA (siRNA) provides promising therapeutic perspectives, nanocarriers for drug and siRNA co-delivery present new alternatives for cancer therapy.

Methods: A co-delivery nanosystem for methotrexate (MTX) or gamma-glutamylated derivatives (gE -MTX and gE -MTX) and antitumoral EG5 siRNA has been developed utilizing the sequence defined cationic lipo-oligomers 454, 1021 and 1027. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3041DOI Listing
July 2018
22 Reads

Efficacy and safety of a clinically relevant foamy vector design in human hematopoietic repopulating cells.

J Gene Med 2018 Jul 19;20(7-8):e3028. Epub 2018 Jul 19.

Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, WSU Spokane, Spokane, WA, USA.

Background: Previous studies have shown that foamy viral (FV) vectors are a promising alternative to gammaretroviral and lentiviral vectors and also that insulators can improve FV vector safety. However, in a previous analysis of insulator effects on FV vector safety, strong viral promoters were used to elicit genotoxic events. In the present study, we developed and analyzed the efficacy and safety of a high-titer, clinically relevant FV vector driven by the housekeeping promoter elongation factor-1α and insulated with an enhancer blocking A1 insulator (FV-EGW-A1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368973PMC
July 2018
3 Reads

Comparative analysis and optimization of protocols for producing recombinant lentivirus carrying the anti-Her2 chimeric antigen receptor gene.

J Gene Med 2018 Jul 6;20(7-8):e3027. Epub 2018 Jul 6.

Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Background: The production of anti-Her2 chimeric antigen receptor (CAR) T cells needs to be optimized to make it a reliable therapy.

Methods: Three types of lentiviral vectors expressing anti-Her2 CAR together with packaging plasmids were co-transfected into 293 T-17 cells. The vector with the best packaging efficiency was selected, and the packaging cell culture system and packaging plasmid system were optimized. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3027
Publisher Site
http://dx.doi.org/10.1002/jgm.3027DOI Listing
July 2018
22 Reads

Human interleukin-10 delivered intrathecally by self-complementary adeno-associated virus 8 induces xenogeneic transgene immunity without clinical neurotoxicity in swine.

J Gene Med 2018 Jul 15;20(7-8):e3026. Epub 2018 Jun 15.

Departments of Anesthesiology and Oncology, Mayo Clinic, Translational Science Track, Mayo Graduate School, Rochester, MN, USA.

Introduction: Intrathecal interleukin (IL)-10 delivered by plasmid or viral gene vectors has been proposed for clinical testing because it is effective for chronic pain in rodents, is a potential therapeutic for various human diseases, and was found to be nontoxic in dogs, when the human IL-10 ortholog was tested. However, recent studies in swine testing porcine IL-10 demonstrated fatal neurotoxicity. The present study aimed to deliver vector-encoded human IL-10 in swine, measure expression of the transgene in cerebrospinal fluid and monitor animals for signs of neurotoxicity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105440PMC
July 2018
5 Reads

Palmitoylated phosphodiester gapmer designs with albumin binding capacity and maintained in vitro gene silencing activity.

J Gene Med 2018 Jul 25;20(7-8):e3025. Epub 2018 Jun 25.

The Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark.

Background: Antisense gapmer oligonucleotide drugs require delivery and biodistribution enabling technologies to increase in vivo efficacy. An attractive approach is their binding and consequent transport by the endogenous human serum albumin pool as mediated by fatty acid incorporation into the gapmer design.

Methods: The present study investigated the effect of palmitoyl modification and position on albumin-binding, cellular uptake and in vitro gene silencing of gapmers with either a phosphorothioate (PS) or phosphodiester (PO) backbone. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3025DOI Listing
July 2018
5 Reads

Soluble Fgl2 restricts autoimmune hepatitis progression via suppressing Tc17 and conventional CD8+ T cell function.

J Gene Med 2018 Jul 19;20(7-8):e3023. Epub 2018 Jun 19.

Institute of Infectious Disease, Tongji Hospital of Tongji Medical College, Wuhan, China.

Background: Autoimmune hepatitis (AIH) is an inflammatory disease caused by an aberrant immune response to hepatic self-antigens in which regulatory T cells (Tregs) are critical for maintaining immunosupression. The soluble form of fibrinogen-like protein 2 (sFGL2), a novel effector molecule of Treg, is rarely investigated in AIH. In the present study, we dissected the role of sFGL2 in autoimmune hepatitis and its potential mechanism underlying AIH progression. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3023DOI Listing
July 2018
4 Reads

The VEGFA -1154G/A polymorphism is associated with reduced risk of rheumatoid arthritis but not with systemic lupus erythematosus in Mexican women.

J Gene Med 2018 Jun 5;20(6):e3024. Epub 2018 Jun 5.

Laboratorio de Genómica del Cáncer, Instituto Nacional de Medicina Genómica, Mexico City, Mexico.

Background: Levels of circulating vascular endothelial growth factor (VEGF) (a potent endothelial-cell-specific angiogenic factor) have been correlated with disease activity in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). In addition, several single nucleotide polymorphisms (SNPs), including the VEGFA -2578C/A, have been associated with RA in some populations. By contrast, the role of different VEGFA SNPs in the susceptibility to SLE has received little attention. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3024DOI Listing
June 2018
6 Reads

MiR-21 ameliorates age-associated skin wound healing defects in mice.

J Gene Med 2018 Jun 3;20(6):e3022. Epub 2018 May 3.

Institute of Combined Injury, State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Army Military Medical University, Chongqing, China.

Background: The cellular and molecular mechanisms responsible for the age-associated delay of cutaneous wound healing are still not well understood. Previous studies have shown that miR-21 plays key roles during skin wound healing. We presumed that dysregulation of miR-21 may be involved in age-associated defects in wound healing and that miR-21 may be one potential therapeutic target by which to ameliorate wound defects in elderly subjects. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jgm.3022
Publisher Site
http://dx.doi.org/10.1002/jgm.3022DOI Listing
June 2018
37 Reads