1,430,107 results match your criteria Journal of Experimental & Clinical Cancer Research [Journal]


Atomistic molecular dynamics simulations of the LCST conformational transition in poly(N-vinylcaprolactam) in water.

J Mol Graph Model 2019 Apr 12;90:51-58. Epub 2019 Apr 12.

School of Chemistry, V.N. Karazin Kharkiv National University, 4 Svobody Square, Kharkiv, 61022, Ukraine. Electronic address:

Thermoresponsive poly(N-vinylcaprolactam) (PVCL) has received growing interest due to a temperature-induced phase transition, which switches its solubility in aqueous solutions. However, the lower critical solution temperature (LCST) of PVCL is greatly influenced by the molecular weight, morphology and the environment. Therefore, despite of numerous experimental studies of the thermal response of PVCL, a driving force and a molecular origin of conformation transitions in solution remain far less studied. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10933263193010
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http://dx.doi.org/10.1016/j.jmgm.2019.04.004DOI Listing
April 2019
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miR-125b Upregulates miR-34a and Sequentially Activates Stress Adaption and Cell Death Mechanisms in Multiple Myeloma.

Mol Ther Nucleic Acids 2019 Mar 13;16:391-406. Epub 2019 Mar 13.

Department of Precision Medicine, University of Campania "Luigi Vanvitelli," 80138 Naples, Italy; IRGS, Biogem, Molecular and Precision Oncology Laboratory, Via Camporeale, 83031 Ariano Irpino, Italy. Electronic address:

miR-125b, ubiquitously expressed and frequently dysregulated in several tumors, has gained special interest in the field of cancer research, displaying either oncogenic or oncosuppressor potential based on tumor type. We have previously demonstrated its tumor-suppressive role in multiple myeloma (MM), but the analysis of molecular mechanisms needs additional investigation. The purpose of this study was to explore the effects of miR-125b and its chemically modified analogs in modulating cell viability and cancer-associated molecular pathways, also focusing on the functional aspects of stress adaptation (autophagy and senescence), as well as programmed cell death (apoptosis). Read More

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http://dx.doi.org/10.1016/j.omtn.2019.02.023DOI Listing

A quencher-free DNAzyme beacon for fluorescently sensing uranyl ions via embedding 2-aminopurine.

Biosens Bioelectron 2019 Apr 15;135:166-172. Epub 2019 Apr 15.

Institute of Materials, China Academy of Engineering Physics, Jiangyou, Mianyang, 621907, Sichuan, PR China.

DNAzyme-based fluorescent probes have provided valuable protocols for detecting uranium, one of the most common radioactive contaminants in the environment, with ultra-high selectivity and sensitivity. Designing novel DNAzyme beacons to update the mode of fluorescence reporting and/or quenching will continuously enhance "turn-on" sensing performance as well as promote actual application of the biological probes. In this work, we developed a novel quencher-free DNAzyme beacon by embedding fluorescent 2-aminopurine for rapid detection of uranyl ion. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09565663193030
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http://dx.doi.org/10.1016/j.bios.2019.04.021DOI Listing
April 2019
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Multi-environment evaluation of oil accumulation pattern parameters in olive.

Plant Physiol Biochem 2019 Apr 14;139:485-494. Epub 2019 Apr 14.

IFAPA Centro "Alameda del Obispo", Avda Menéndez Pidal s/n, 14004, Córdoba, Spain. Electronic address:

The pattern of olive oil accumulation is of paramount importance in olive because its influence in determining final oil yield and optimal harvesting date. In this work, we have evaluated the genetic and environment influence on the olive oil accumulation pattern. For that purpose, a set of cultivars and breeding selections planted in a multi-environment trial was evaluated in two consecutive harvest seasons. Read More

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http://dx.doi.org/10.1016/j.plaphy.2019.04.016DOI Listing

Carbonic anhydrase activity as a potential biomarker for acute exposure to copper in corals.

Chemosphere 2019 Apr 15;227:598-605. Epub 2019 Apr 15.

Instituto Coral Vivo, Rua dos Coqueiros, Parque Yaya, Santa Cruz Cabrália, BA, 45807-000, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália, Km 8, Rio Grande, RS, 96203-900, Brazil. Electronic address:

Coral reefs are subjected to climate change and are severely impacted by human activities, with copper (Cu) being a relevant physiological stressor for corals at local scale. The ecological relevance of parameters measured at biochemical or cellular level is now considered an extremely important feature in environmental studies, and can be used as early warning signs of environmental degradation. In this context, the effects of acute exposure (96 h) to Cu were assessed on the maximum photochemical efficiency of zooxanthellae (Fv/Fm) and on the activity of key enzymes [carbonic anhydrase (CA) and Ca-ATPase] involved in coral physiology using the scleractinian coral Mussismilia harttii as a biological model. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00456535193074
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http://dx.doi.org/10.1016/j.chemosphere.2019.04.089DOI Listing
April 2019
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Bavachin attenuates LPS-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages.

Phytomedicine 2018 Dec 10;59:152785. Epub 2018 Dec 10.

Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Electronic address:

Background: Bavachin is a natural product isolated from Psoralea corylifolia L. that has been applied as a traditional medicine in Asian countries. However, the anti-inflammatory effects of bavachin on LPS-induced inflammation and NLRP3 inflammasome activation by macrophages remain unclear. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.12.008DOI Listing
December 2018

5-Hydroxytryptamine1a receptors on tumour cells induce immune evasion in lung adenocarcinoma patients with depression via autophagy/pSTAT3.

Eur J Cancer 2019 Apr 19;114:8-24. Epub 2019 Apr 19.

Department of Oncology, XiangYang Central Hospital, Hubei University of Arts and Science, XiangYang 441021, China. Electronic address:

Background: Cancer patients frequently suffer from fatigue and depression. Dysregulation of the immune system, tumour recurrence and metastasis are more common in cancer patients with depression. 5-Hydroxytryptamine (5-HT), a neurotransmitter, contributes to immune evasion in lung adenocarcinoma patients by activating 5-HTRs, but the mechanism for this phenomenon is still unclear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09598049193021
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http://dx.doi.org/10.1016/j.ejca.2019.03.017DOI Listing

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation.

Stem Cell Res 2019 Apr 13;37:101437. Epub 2019 Apr 13.

Coronary Care Unit, Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Department of Molecular Medicine, Unit of Cardiology, Università degli studi di Pavia, Pavia, Italy; Department of Medicine, University of Cape Town, Cape Town, South Africa. Electronic address:

We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18735061193006
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http://dx.doi.org/10.1016/j.scr.2019.101437DOI Listing
April 2019
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Abdominal Pain in Children Develops With Age and Increases With Psychosocial Factors.

Clin Gastroenterol Hepatol 2019 Apr 19. Epub 2019 Apr 19.

Div of Pediatrics, Dept of Clinical and Experimental Medicine, Linköping university SE 58185 Linköping, Sweden; Crown Princess Victoria Children´s Hospital, Region Östergötland, Linköping, Sweden.

Background & Aims: Functional gastrointestinal disorders are highly prevalent, cause significant suffering, and are costly to society. Pain is a central feature of 2 of the most common functional gastrointestinal disorders-irritable bowel syndrome and functional dyspepsia. Although these disorders have been well studied in adults, their etiology is poorly understood. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15423565193042
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http://dx.doi.org/10.1016/j.cgh.2019.04.036DOI Listing
April 2019
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Mechanosensitive MiRs Regulated by Anabolic and Catabolic Loading of Human Cartilage.

Osteoarthritis Cartilage 2019 Apr 19. Epub 2019 Apr 19.

Research Centre for Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany. Electronic address:

Objective: Elucidation of whether miRs are involved in mechanotransduction pathways by which cartilage is maintained or disturbed has a particular importance in our understanding of osteoarthritis (OA) pathophysiology. The aim was to investigate whether mechanical loading influences global miR-expression in human chondrocytes and to identify mechanosensitive miRs responding to beneficial and non-beneficial loading regimes as potential to obtain valuable diagnostic or therapeutic targets to advance OA-treatment.

Method: Mature tissue-engineered human cartilage was subjected to two distinct loading regimes either stimulating or suppressing proteoglycan-synthesis, before global miR microarray analysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10634584193095
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http://dx.doi.org/10.1016/j.joca.2019.04.010DOI Listing
April 2019
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Reappraisal of probiotics' safety in human.

Food Chem Toxicol 2019 Apr 19. Epub 2019 Apr 19.

Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Contrary to the safe usage of probiotics for years, their threat is still worthy of attention. Several risks have been explained or mentioned in the case reports, clinical trials and experimental studies. Due to a large number of probiotic products worldwide, the certainty of the safety of such products is a matter of concern. Read More

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http://dx.doi.org/10.1016/j.fct.2019.04.032DOI Listing
April 2019
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A study on Pressure Lowering Effect of the Multilayer Stent.

Ann Vasc Surg 2019 Apr 19. Epub 2019 Apr 19.

Department of Cardiology Ward 1, Beijing Anzhen Hospital of Capital Medical University, Beijing 100029, China. Electronic address:

Objective: To investigate the hemodynamic changes of the blood flow in the aneurysm model after the multilayer stent placement using fluid dynamic method, in order to analyze the effectiveness and properties of the multilayer stent in the treatment of aortic aneurysms.

Methods: A water tank was filled with 5L of experimental liquid after the circular flow pressure test platform with a glass aneurysm model and a multilayer stent was built. Pressure at the middle part and the distal aneurysm neck part of the model was then measured. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08905096193024
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http://dx.doi.org/10.1016/j.avsg.2019.01.012DOI Listing
April 2019
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The clinical impact of splanchnic ischemia in patients affected by thoracoabdominal aortic aneurysms treated with fenestrated and branched endograft.

Ann Vasc Surg 2019 Apr 19. Epub 2019 Apr 19.

Vascular Surgery, Department of Experimental, Diagnostic and Speciality Medicine, University of Bologna, Policlinico Sant'Orsola-Malpighi Bologna - Italy.

Introduction: Fenestrated/branched endografts (FB-EVAR) are valid options to treat thoraco-abdominal aortic aneurysms (TAAA). Successful repair requires target-visceral-vessels (TVV) manipulation with possible splanchnic ischemia. Aim of the study was to evaluate the clinical impact of splanchnic ischemia occurring in FB-EVAR for TAAA. Read More

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http://dx.doi.org/10.1016/j.avsg.2019.01.026DOI Listing

Aspirin-Dependent Platelet Inflammatory Inhibition In Healthy Subjects Decreases Nlrp1 Inflammasome.

Ann Vasc Surg 2019 Apr 19. Epub 2019 Apr 19.

Angiology and Vascular Surgery Department. Hospital Universitario de Getafe. Madrid. Spain.

Objective: Inflammation and endothelial dysfunction are implicated in the onset of atherosclerosis. Inflammasome activation takes part in the pathogenesis of the atherosclerotic disease. This study investigated the influence of platelet inflammatory inhibition on the transcription of intracitosolic NLRP-1 inflammasome in endothelial cells. Read More

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http://dx.doi.org/10.1016/j.avsg.2019.02.008DOI Listing

The EXACT protocol: a multi-centre, single-blind, randomised, parallel-group, controlled trial to determine whether early oxygen titration improves survival to hospital discharge in adult OHCA patients.

Resuscitation 2019 Apr 19. Epub 2019 Apr 19.

Department of Epidemiology and Preventive Medicine, Monash University, Australia; Prehospital, Resuscitation and Emergency Care Research Unit, Curtin University, Australia; Alfred Hospital, Australia; Ambulance Victoria, Australia; Department of Community Emergency Health and Paramedic Practice, Monash University, Australia; SA Ambulance Service, Australia; Flinders University, Australia; St John Ambulance Western Australia, Australia; Warwick University, United Kingdom.

Background: Experimental and observational research suggests hyperoxia following resuscitation from cardiac arrest is associated with neurological injury and worse clinical outcomes. This paper describes the rationale and design of the EXACT trial. EXACT aims to determine whether reducing oxygen in the acute phase of post-resuscitation care for out-of-hospital cardiac arrest (OHCA) improves survival. Read More

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http://dx.doi.org/10.1016/j.resuscitation.2019.04.023DOI Listing

Effect of Endotracheal Intubation and Supraglottic Airway Device Placement during Cardiopulmonary Resuscitation on Carotid Blood Flow over Resuscitation Time: An Experimental Porcine Cardiac Arrest Study.

Resuscitation 2019 Apr 19. Epub 2019 Apr 19.

Department of Emergency Medicine, Seoul National University Boramae Medical Center, Seoul, Republic of Korea; Laboratory of Emergency Medical Services, Seoul National University Hospital Biomedical Research Institute, Seoul, Republic of Korea. Electronic address:

Background: Supraglottic airway devices (SGDs) are widely used during the resuscitation of out-of-hospital cardiac arrest (OHCA). The effect of SGDs on carotid blood flow (CBF) as resuscitation time passes is controversial. We assessed the effects of endotracheal intubation (ETI) and 3 types of SGD placement on CBF over time in prolonged resuscitation through an experimental porcine cardiac arrest study. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009572193013
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http://dx.doi.org/10.1016/j.resuscitation.2019.04.020DOI Listing
April 2019
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Differential expression patterns of sodium potassium ATPase alpha and beta subunit isoforms in mouse brain during postnatal development.

Neurochem Int 2019 Apr 19. Epub 2019 Apr 19.

Ruhr University Bochum, Faculty of Chemistry and Biochemistry, Biochemistry II, Bochum, Germany; Ruhr University Bochum, International Graduate School for Neuroscience, Germany. Electronic address:

The sodium potassium ATPase (Na/K ATPase) is essential for the maintenance of a low intracellular Na and a high intracellular K concentration. Loss of function of the Na/K ATPase due to mutations in Na/K ATPase genes, anoxic conditions, depletion of ATP or inhibition of the Na/K ATPase function using cardiac glycosides such as digitalis, causes a depolarization of the resting membrane potential. While in non-excitable cells, the uptake of glucose and amino acids is decreased if the function of the Na/K ATPase is compromised, in excitable cells the symptoms range from local hyper-excitability to inactivating depolarization. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01970186183045
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http://dx.doi.org/10.1016/j.neuint.2019.04.009DOI Listing
April 2019
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Protein nanotubes as state-of-the-art nanocarriers: Synthesis methods, simulation and applications.

J Control Release 2019 Apr 19. Epub 2019 Apr 19.

Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran. Electronic address:

Application of food proteins as a tool to form nanostructures (especially nanotubular shapes) has been an interesting topic for both the food and pharmaceutical sectors. Organic and protein nanostructures have better biocompatibility and biodegradability compared to inorganic counterparts like carbon nanotubes; in addition, they can undergo surface modifications. Several organic nanotubes have been developed, meanwhile, the engineered protein nanotubes in the food science have been prepared from α-lactalbumin, ovalbumin, cyclic peptide nanotubes, collagen, bovine serum albumin, lysozyme and hydrophobins which are of great interest to be applied in the food industry considering their outstanding properties. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.04.026DOI Listing

Urotherapy in the treatment of children and adolescents with bladder and bowel dysfunction: a systematic review.

J Pediatr (Rio J) 2019 Apr 19. Epub 2019 Apr 19.

Universidade de Brasília (UnB), Departamento de Enfermagem, Brasília, DF, Brazil; Society for Pediatric Urology (SPU), United States; McGill University, Ingram School of Nursing, Quebec, Canada; Shriners Hospital for Children, Quebec, Canada.

Objective: To identify and describe the protocols and clinical outcomes of urotherapy interventions in children and adolescents with bladder bowel dysfunction.

Method: Systematic review carried out in June 2018 on MEDLINE/PubMed, CINAHL, EMBASE, SciELO, Cochrane, and PsycInfo databases. Clinical trials and quasi-experimental studies carried out in the last ten years in children and/or adolescents with bladder and bowel symptoms and application of at least one component of urotherapy were included. Read More

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http://dx.doi.org/10.1016/j.jped.2019.02.007DOI Listing

An Evaluation of Mepolizumab for the Treatment of Severe Asthma.

Expert Opin Biol Ther 2019 Apr 22. Epub 2019 Apr 22.

c Department of Clinical and Experimental Medicine , University of Catania , Catania (CT) , Italy.

Introduction Asthma is considered one of the most common chronic conditions globally, characterized by variable airflow obstruction and symptoms. Severe asthma is diagnosed when asthma control requires high-intensity therapy or continues to remain uncontrolled despite treatment. Eosinophilic inflammation is known to be perpetuated by the activity of IL-5 in a proportion of severe asthma subjects, and targeting IL-5 may offer a therapeutic option. Read More

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https://www.tandfonline.com/doi/full/10.1080/14712598.2019.1
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http://dx.doi.org/10.1080/14712598.2019.1610382DOI Listing
April 2019
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Perspective and guidelines for metaproteomics in microbiome studies.

J Proteome Res 2019 Apr 22. Epub 2019 Apr 22.

The microbiome is emerging as a prominent factor affecting human health and its dysbiosis is associated with various diseases. Compositional profiling of microbiome is increasingly being supplemented with functional characterization. Metaproteomics is intrinsically focused on functional changes and therefore will be an important tool in those studies of the human microbiome. Read More

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http://dx.doi.org/10.1021/acs.jproteome.9b00054DOI Listing

Assessing therapist development: Reliability and validity of the Supervisee Levels Questionnaire (SLQ-R).

J Clin Psychol 2019 Apr 22. Epub 2019 Apr 22.

Department of Clinical Psychology and Psychotherapy, University of Potsdam, Potsdam, Germany.

Background: Therapist development is a crucial target for clinical training in order to ensure high-quality psychotherapy. A major challenge in examining therapeutic development is the assessment of developmental processes. The Supervisee Levels Questionnaire (SLQ-R) was analyzed in this study to examine its validity, reliability, and underlying dimensional structure. Read More

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http://dx.doi.org/10.1002/jclp.22794DOI Listing

Development of a Pde6b Gene Knockout Rat Model for Studies of Degenerative Retinal Diseases.

Invest Ophthalmol Vis Sci 2019 Apr;60(5):1519-1526

Department of Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

Purpose: To describe the phenotypes of a newly developed Pde6b-deficient rat model of retinal degeneration.

Methods: Pde6b knockout rats were produced by CRISPR-Cpf1 technology. Pde6b knockout rats were evaluated for ocular abnormalities by comparison with wild-type eyes. Read More

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http://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.1
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http://dx.doi.org/10.1167/iovs.18-25556DOI Listing
April 2019
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Twin arginine translocation, ammonia incorporation, and polyamine biosynthesis are crucial for Proteus mirabilis fitness during bloodstream infection.

PLoS Pathog 2019 Apr 22;15(4):e1007653. Epub 2019 Apr 22.

Department of Microbiology and Immunology; University of Michigan Medical School; Ann Arbor, MI, United States of America.

The Gram-negative bacterium Proteus mirabilis is a common cause of catheter-associated urinary tract infections (CAUTI), which can progress to secondary bacteremia. While numerous studies have investigated experimental infection with P. mirabilis in the urinary tract, little is known about pathogenesis in the bloodstream. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007653DOI Listing

Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation.

PLoS One 2019 22;14(4):e0215678. Epub 2019 Apr 22.

Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Mesenchymal stem cells (MSCs) are of special interest due their potential clinical use in cell-based therapy. Therapies engaging MSCs are showing increasing promise in the cancer treatment and anticancer drug screening applications. A multitude of growth factors and cytokines secreted from these cells are known to give such multifunctional properties, but details of their role are yet to be absolutely demonstrated. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215678PLOS

Destabilization of chromosome structure by histone H3 lysine 27 methylation.

PLoS Genet 2019 Apr 22;15(4):e1008093. Epub 2019 Apr 22.

Environmental Genomics, Christian-Albrechts University, Kiel, Germany.

Chromosome and genome stability are important for normal cell function as instability often correlates with disease and dysfunction of DNA repair mechanisms. Many organisms maintain supernumerary or accessory chromosomes that deviate from standard chromosomes. The pathogenic fungus Zymoseptoria tritici has as many as eight accessory chromosomes, which are highly unstable during meiosis and mitosis, transcriptionally repressed, show enrichment of repetitive elements, and enrichment with heterochromatic histone methylation marks, e. Read More

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http://dx.plos.org/10.1371/journal.pgen.1008093
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http://dx.doi.org/10.1371/journal.pgen.1008093DOI Listing
April 2019
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Temporal pattern separation in hippocampal neurons through multiplexed neural codes.

PLoS Comput Biol 2019 Apr 22;15(4):e1006932. Epub 2019 Apr 22.

Department of Neuroscience, University of Wisconsin-Madison, WI, United States of America.

Pattern separation is a central concept in current theories of episodic memory: this computation is thought to support our ability to avoid confusion between similar memories by transforming similar cortical input patterns of neural activity into dissimilar output patterns before their long-term storage in the hippocampus. Because there are many ways one can define patterns of neuronal activity and the similarity between them, pattern separation could in theory be achieved through multiple coding strategies. Using our recently developed assay that evaluates pattern separation in isolated tissue by controlling and recording the input and output spike trains of single hippocampal neurons, we explored neural codes through which pattern separation is performed by systematic testing of different similarity metrics and various time resolutions. Read More

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http://dx.doi.org/10.1371/journal.pcbi.1006932DOI Listing

BOFdat: Generating biomass objective functions for genome-scale metabolic models from experimental data.

PLoS Comput Biol 2019 Apr 22;15(4):e1006971. Epub 2019 Apr 22.

Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.

Genome-scale metabolic models (GEMs) are mathematically structured knowledge bases of metabolism that provide phenotypic predictions from genomic information. GEM-guided predictions of growth phenotypes rely on the accurate definition of a biomass objective function (BOF) that is designed to include key cellular biomass components such as the major macromolecules (DNA, RNA, proteins), lipids, coenzymes, inorganic ions and species-specific components. Despite its importance, no standardized computational platform is currently available to generate species-specific biomass objective functions in a data-driven, unbiased fashion. Read More

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http://dx.doi.org/10.1371/journal.pcbi.1006971DOI Listing

Acute Effect of Drop-Set, Traditional, and Pyramidal Systems in Resistance Training on Neuromuscular Performance in Trained Adults.

J Strength Cond Res 2019 Apr 17. Epub 2019 Apr 17.

Graduate Program in Physical Education of Federal University of Paraíba (UFPB), Paraíba, Brazil.

Costa, BDV, Ferreira, MEC, Gantois, P, Kassiano, W, Paes, ST, de Lima-Júnior, D, Cyrino, ES, and Fortes, LS. Acute effect of drop-set, traditional, and pyramidal systems in resistance training on neuromuscular performance in trained adults. J Strength Cond Res XX(X): 000-000, 2019-The aim of this study was to analyze the acute effects of resistance training (RT) systems on lower- and upper-limb performance in trained adults. Read More

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http://dx.doi.org/10.1519/JSC.0000000000003150DOI Listing

Anaerobic Threshold in a Water-Based Exercise: Agreement Between Heart Rate Deflection Point and Lactate Threshold Methods.

J Strength Cond Res 2019 Apr 17. Epub 2019 Apr 17.

Laboratory of Neuromuscular Assessment, Department of Sports, Federal University of Pelotas, Pelotas, Brazil.

Alberton, CL, Andrade, LS, Pinheiro, RB, and Pinto, SS. Anaerobic threshold in a water-based exercise: agreement between heart rate deflection point and lactate threshold methods. J Strength Cond Res XX(X): 000-000, 2019-The purpose of this study was to compare and assess the agreement of the heart rate (HR) and rating of perceived exertion (RPE) corresponding to the anaerobic threshold (AT) between HR deflection point (HRDP) and lactate threshold (LT) methods during a water-based exercise in young men. Read More

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http://dx.doi.org/10.1519/JSC.0000000000003161DOI Listing

Extent of Ischemic Brain Injury After Thrombotic Stroke Is Independent of the NLRP3 (NACHT, LRR and PYD Domains-Containing Protein 3) Inflammasome.

Stroke 2019 May;50(5):1232-1239

From the Division of Neuroscience and Experimental Psychology (E.L., J.B., G.C., S.M.A., D.B.), School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, United Kingdom.

Background and Purpose- A major process contributing to cell death in the ischemic brain is inflammation. Inflammasomes are multimolecular protein complexes that drive inflammation through activation of proinflammatory cytokines, such as IL (interleukin)-1β. Preclinical evidence suggests that IL-1β contributes to a worsening of ischemic brain injury. Read More

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http://dx.doi.org/10.1161/STROKEAHA.118.023620DOI Listing

Siponimod (BAF312) Treatment Reduces Brain Infiltration but Not Lesion Volume in Middle-Aged Mice in Experimental Stroke.

Stroke 2019 May;50(5):1224-1231

From the Department of Neurology (A.V., J.R., A.D., J.S.), University Medicine, Greifswald, Germany.

Background and Purpose- The contribution of neuroinflammation and, in particular, the infiltration of the brain by lymphocytes is increasingly recognized as a substantial pathophysiological mechanism after stroke. The interaction of lymphocytes with endothelial cells and platelets, termed thromboinflammation, fosters microvascular dysfunction and secondary infarct growth. Siponimod is an S1PR (sphingosine-1-phosphate receptor) modulator, which blocks the egress of lymphocytes from lymphoid organs and has demonstrated beneficial effects in multiple sclerosis treatment. Read More

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http://dx.doi.org/10.1161/STROKEAHA.118.023667DOI Listing

Secondary Bleeding During Acute Experimental Intracerebral Hemorrhage.

Stroke 2019 May;50(5):1210-1215

From the Neurovascular Research Laboratory, Department of Radiology (F.S., M.B., T.Q., I.T., P.F., C.A.), Massachusetts General Hospital, Harvard Medical School, Charlestown.

Background and Purpose- Mechanisms contributing to acute hematoma growth in intracerebral hemorrhage are not well understood. Neuropathological studies suggest that the initial hematoma may create mass effect that can tear vessels in the vicinity by shearing, causing further bleeding and hematoma growth. Methods- To test this in mice, we simulated initial intracerebral hemorrhage by intrastriatal injection of a liquid polymer that coagulates upon contact with tissue and measured the presence and volume of bleeding secondary to the mass effect using Hemoglobin ELISA 15 minutes after injection. Read More

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https://www.ahajournals.org/doi/10.1161/STROKEAHA.118.021732
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http://dx.doi.org/10.1161/STROKEAHA.118.021732DOI Listing
May 2019
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Arteriopathy Influences Pediatric Ischemic Stroke Presentation, but Sickle Cell Disease Influences Stroke Management.

Stroke 2019 May;50(5):1089-1094

Departments of Pediatrics, Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center at Dallas and Children's Health Dallas (M.M.D.).

Background and Purpose- Sickle cell disease (SCD) and arteriopathy are pediatric stroke risk factors that are not mutually exclusive. The relative contributions of sickled red blood cells and arteriopathy to stroke risk are unknown, resulting in unclear guidelines for primary and secondary stroke prevention when both risk factors are present. We hypothesized that despite similarities in clinical presentation and radiographic appearance of arteriopathies, stroke evaluation and management differ in children with SCD compared with those without SCD. Read More

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https://www.ahajournals.org/doi/10.1161/STROKEAHA.118.022800
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http://dx.doi.org/10.1161/STROKEAHA.118.022800DOI Listing
May 2019
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Selection of adjuvants for vaccines targeting specific pathogens.

Expert Rev Vaccines 2019 Apr 22:1-17. Epub 2019 Apr 22.

a VIDO-InterVac , University of Saskatchewan , Saskatoon , Canada.

Introduction: Adjuvants form an integral component in most of the inactivated and subunit vaccine formulations. Careful and proper selection of adjuvants helps in promoting appropriate immune responses against target pathogens at both innate and adaptive levels such that protective immunity can be elicited. Areas covered: Herein, we describe the recent progress in our understanding of the mode of action of adjuvants that are licensed for use in human vaccines or in clinical or pre-clinical stages at both innate and adaptive levels. Read More

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http://dx.doi.org/10.1080/14760584.2019.1604231DOI Listing

Balance Training in Virtual Reality Promotes Performance Improvement but not Transfer to Postural Control in People with Chronic Stroke.

Games Health J 2019 Apr 22. Epub 2019 Apr 22.

2 Institute of Psychology, University of São Paulo, São Paulo, Brazil.

In people with chronic stroke, we investigated the transfer of gains obtained after balance training with virtual reality (VR) to an untrained task with similar balance demands. This study included 29 people with chronic stroke randomized into two groups: experimental (EG,  = 16) and control (CG,  = 13). The EG performed three sessions of balance training with VR using a platform-based videogame (Nintendo Wii Fit system™) for 1 week. Read More

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http://dx.doi.org/10.1089/g4h.2018.0075DOI Listing

Scaled Alternating Steepest Descent Algorithm Applied for Protein Structure Determination from Nuclear Magnetic Resonance Data.

J Comput Biol 2019 Apr 22. Epub 2019 Apr 22.

Center for Quantum Technology Research, School of Physics, Beijing Institute of Technology, Beijing, China.

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https://www.liebertpub.com/doi/10.1089/cmb.2019.0013
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http://dx.doi.org/10.1089/cmb.2019.0013DOI Listing
April 2019
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Insights on the Stability and Cationic Nonstoichiometry of CuFeO Delafossite.

Inorg Chem 2019 Apr 22. Epub 2019 Apr 22.

CIRIMAT , Université de Toulouse, CNRS, Université Paul Sabatier , 118 Route de Narbonne , 31062 Toulouse, Cedex 9 , France.

CuFeO, the structure prototype of the delafossite family, has received renewed interest in recent years. Thermodynamic modeling and several experimental Cu-Fe-O system investigations did not focus specifically on the possible nonstoichiometry of this compound, which is, nevertheless, a very important optimization factor for its physicochemical properties. In this work, through a complete set of analytical and thermostructural techniques from 50 to 1100 °C, a fine reinvestigation of some specific regions of the Cu-Fe-O phase diagram under air was carried out to clarify discrepancies concerning the delafossite CuFeO stability region as well as the eutectic composition and temperature for the reaction L = CuFeO + CuO. Read More

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http://pubs.acs.org/doi/10.1021/acs.inorgchem.9b00651
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http://dx.doi.org/10.1021/acs.inorgchem.9b00651DOI Listing
April 2019
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N-Methyl-d-aspartate receptor open-channel blockers memantine and magnesium modulate nociceptive trigeminovascular neurotransmission in rats.

Eur J Neurosci 2019 Apr 22. Epub 2019 Apr 22.

Department of Neurology, University of California, San Francisco, San Francisco, CA, USA.

Experimental and clinical studies suggest that the low-affinity N-methyl-d-aspartate (NMDA) receptor open-channel blockers Mg and memantine are effective in reducing trigeminal nociceptive activation. The aim of the present study was to investigate the apparent effectiveness of these channel blockers using a model of trigeminal activation in vivo. Rats were anesthetized before electrically stimulating the dura mater adjacent the middle meningeal artery. Read More

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http://dx.doi.org/10.1111/ejn.14423DOI Listing

Ethanol Exposure Increases miR-140 in Extracellular Vesicles: Implications for Fetal Neural Stem Cell Proliferation and Maturation.

Alcohol Clin Exp Res 2019 Apr 22. Epub 2019 Apr 22.

Department of Neuroscience and Experimental Therapeutics, Texas A&M University Health Science Center, Bryan, TX, USA.

Background: Neural stem cells (NSCs) generate most of the neurons of the adult brain in humans, during the mid-first through second trimester period. This critical neurogenic window is particularly vulnerable to prenatal alcohol exposure, which can result in diminished brain growth. Previous studies showed that ethanol exposure does not kill NSCs, but, rather, results in their depletion by influencing cell cycle kinetics and promoting aberrant maturation, in part, by altering NSC expression of key neurogenic miRNAs. Read More

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http://dx.doi.org/10.1111/acer.14066DOI Listing

Protein-engineered molecules carrying GAD65 epitopes and targeting CD35 selectively down-modulate disease-associated human B lymphocytes.

Clin Exp Immunol 2019 Apr 22. Epub 2019 Apr 22.

Laboratory of Experimental Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria.

Background: Type 1 diabetes mellitus is an autoimmune metabolic disorder characterized by chronic hyperglycemia, presence of autoreactive T and B cells and autoantibodies against self-antigens. A membrane-bound enzyme on the pancreatic beta-cells, GAD 65, is one of the main autoantigens in type 1 diabetes. Autoantibodies against GAD65 are potentially involved in beta-cells destruction and decline of pancreatic functions. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cei.13305
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http://dx.doi.org/10.1111/cei.13305DOI Listing
April 2019
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Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens.

J Vis Exp 2019 Apr 5(146). Epub 2019 Apr 5.

Department of Biochemistry and Molecular Biology, Center for Molecular Medicine and Complex Carbohydrate Research Center, University of Georgia;

A key aspect of the immune response to bacterial colonization of the host is phagocytosis. An opsonophagocytic killing assay (OPKA) is an experimental procedure in which phagocytic cells are co-cultured with bacterial units. The immune cells will phagocytose and kill the bacterial cultures in a complement-dependent manner. Read More

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http://dx.doi.org/10.3791/59400DOI Listing

Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster (Nephrops norvegicus).

J Vis Exp 2019 Apr 8(146). Epub 2019 Apr 8.

Institute of Marine Sciences, Spanish National Research Council (CSIC).

We present a protocol related to a video-tracking technique based on the background subtraction and image thresholding that makes it possible to individually track cohoused animals. We tested the tracking routine with four cohoused Norway lobsters (Nephrops norvegicus) under light-darkness conditions for 5 days. The lobsters had been individually tagged. Read More

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http://dx.doi.org/10.3791/58515DOI Listing

Studying Brain Function in Children Using Magnetoencephalography.

J Vis Exp 2019 Apr 8(146). Epub 2019 Apr 8.

ARC Centre of Excellence in Cognition and its Disorders, Macquarie University; Department of Cognitive Science, Macquarie University;

Magnetoencephalography (MEG) is a non-invasive neuroimaging technique which directly measures magnetic fields produced by the electrical activity of the human brain. MEG is quiet and less likely to induce claustrophobia compared with magnetic resonance imaging (MRI). It is therefore a promising tool for investigating brain function in young children. Read More

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http://dx.doi.org/10.3791/58909DOI Listing

"Effectiveness of retrobulbar hyaluronidase injection in an iatrogenic blindness rabbit model using hyaluronic acid filler injection".

Plast Reconstr Surg 2019 Apr 15. Epub 2019 Apr 15.

Dept. of Plastic and Reconstructive Surgery, Cheil General Hospital and Women's Healthcare center, Dankook University, College of Medicine, South Korea.

Background: Blindness caused by soft tissue filler injection is the most tragic complication, with no standard treatments until recently. Retrobulbar hyaluronidase injection has been proposed as the treatment, but its effectiveness in visual compromise remains to be determined. The authors aimed to determine the effectiveness of retrobulbar hyaluronidase using soft tissue filler in an iatrogenic blindness animal model. Read More

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http://dx.doi.org/10.1097/PRS.0000000000005716DOI Listing
April 2019
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Effect of Vasopressors on The Macro- and Microcirculation During Systemic Inflammation In Humans in vivo.

Shock 2019 Apr 16. Epub 2019 Apr 16.

Department of Intensive Care Medicine, Radboud university medical center, Nijmegen, the Netherlands.

Aim: Comparing the effects of different vasopressors in septic shock patients is hampered by high heterogeneity and the fact that current guidelines dictate the use of norepinephrine. Herein, we studied the effects of three vasopressor agents, norepinephrine, phenylephrine and vasopressin, on the macro- and microcirculation during experimental human endotoxemia, a standardized, controlled model of systemic inflammation in humans in vivo.

Methods: We performed a randomized controlled study in which 40 healthy male volunteers were assigned to a five-hour infusion of either 0. Read More

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http://dx.doi.org/10.1097/SHK.0000000000001357DOI Listing

Dose-Response Relationship Between Internal Training Load and Changes in Performance During the Preseason in Youth Soccer Players.

J Strength Cond Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Physical Education, State University of Londrina, Londrina, Brazil.

Figueiredo, DH, Figueiredo, DH, Moreira, A, Gonçalves, HR, and Dourado, AC. Dose-response relationship between internal training load and changes in performance during the preseason in youth soccer players. J Strength Cond Res XX(X): 000-000, 2019-The aim of this study was to describe training intensity distribution based on the session rating of perceived exertion (sRPE) and heart rate (HR) methods and examine the dose-response relation between internal training load (ITL) and change in performance of 16 youth soccer players (mean ± SD age: 18. Read More

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http://dx.doi.org/10.1519/JSC.0000000000003126DOI Listing

Neuropathic itch.

Pain 2019 May;160 Suppl 1:S11-S16

Department of Experimental Pain Research, CBTM Mannheim, Heidelberg University, Mannheim, Germany.

Neuropathic itch is clinically important but has received much less attention as compared to neuropathic pain. In the past decade, itch-specific pathways have been characterized on a cellular and molecular level, but their exact role in the pathophysiology of neuropathic itch is still unclear. Traditionally, mutually exclusive theories for itch such as labeled line, temporal/spatial pattern, or intensity theory have been proposed, and experimental studies in mice mainly favor the specificity theory of itch. Read More

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http://dx.doi.org/10.1097/j.pain.0000000000001551DOI Listing

Stem-cell regenerative medicine as applied to the penis.

Curr Opin Urol 2019 Apr 16. Epub 2019 Apr 16.

Department of Urology.

Purpose Of Review: Tissue engineering and regenerative medicine has emerged as a new scientific interdisciplinary field focusing on developing new strategies to repair or recreate tissues and organs. This review gathers findings on erectile dysfunction and, Peyronie's disease from recent preclinical and clinical studies under heading of stem-cell regenerative medicine.

Recent Findings: Over the last 2 years, preclinical studies on rat models demonstrated the tangible beneficial role of stem cells and stromal vascular fraction in the context of preventing fibrosis and restoring erectile function in different animal models of Erectile dysfunction and Peyronie's disease. Read More

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http://dx.doi.org/10.1097/MOU.0000000000000636DOI Listing

The importance of developing relevant animal models to assess existing and new materials.

Curr Opin Urol 2019 Apr 16. Epub 2019 Apr 16.

Centre for Surgical Technologies.

Purpose Of Review: We summarize the recent literature on the use of different animal models for testing existing and new materials for treatment of pelvic organ prolapse.

Recent Findings: A wide spectrum of animal models is being used in urogynecology, both for the study of physiologic and pathophysiologic processes, training in surgical procedures, yet mainly to study the host response to implant materials. The quality of studies is variable, and procedures, read-outs, and reporting are not standardized. Read More

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http://dx.doi.org/10.1097/MOU.0000000000000625DOI Listing
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