1,429,454 results match your criteria Journal of Experimental & Clinical Assisted Reproduction JECAR [Journal]


Effects of Homeopathic Phosphorus on Encephalitozoon cuniculi-Infected Macrophages In-Vitro.

Homeopathy 2019 Apr 18. Epub 2019 Apr 18.

Research Center, Graduating Program of Environmental and Experimental Pathology, Universidade Paulista - UNIP, São Paulo, São Paulo, Brazil.

Introduction:   (), a fungus that acts as an intracellular pathogen, causes a marked neurological syndrome in many host species and is a zoonotic concern. Although no well-established treatment for this syndrome is known, previous successful clinical experience using homeopathic phosphorus has been described in which symptom remission with no mortality occurred in 40/42 animals by means of unknown immunological mechanisms. The latter observation was the main motivation for this study. Read More

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http://dx.doi.org/10.1055/s-0039-1678700DOI Listing

Behavioral and Biochemical Features of the Course and Surgical Treatment of Experimental Obstructive Hydrocephalus in Young Rats.

Dev Neurosci 2019 Apr 18:1-10. Epub 2019 Apr 18.

Division of Pediatric Neurosurgery, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.

Introduction: Hydrocephalus is a multifactorial disease, affecting the dynamics of cerebrospinal fluid (CSF) and leading to severe neurological impairment in children; in spite of the recent advances in hydrocephalus research, it has many physiopathological aspects that still remain poorly understood, especially after treatment.

Objectives: To analyze the clinical, radiological, histopathological, and biochemical aspects of kaolin-induced hydrocephalus in an experimental model, both in the acute phase and after shunt treatment, by means of behavioral tests, magnetic resonance imaging (MRI) scans, histopathological studies, and level of inflammatory interleukins in the CSF.

Methods: Seven-day-old Wistar rats were used and subdivided into three subgroups: treated hydrocephalic (n = 24), untreated hydrocephalic (n = 17), and controls (n = 5). Read More

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http://dx.doi.org/10.1159/000497433DOI Listing

Effects of the Narrative Intervention Program for Preschool Portuguese Children.

Folia Phoniatr Logop 2019 Apr 18:1-7. Epub 2019 Apr 18.

CINTESIS.UA - Center for Health Technology and Services Research (UID/IC/04255/2013) and School of Health Sciences (ESSUA), University of Aveiro, Aveiro, Portugal,

Objective: This article analyzes the contribution of a narrative intervention program in linguistic performance (measured by the percentage of correct consonants [PCC], mean length of utterance [MLU], and auditory memory [AM]) of children.

Patients And Methods: A group of 14 children with primary language impairments participated in this study. The children were divided into two groups (seven children in each): the narrative intervention group (experimental group) and the control group. Read More

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http://dx.doi.org/10.1159/000496793DOI Listing

Longitudinal and transverse magnetoresistance of SrTiO3 with a single closed Fermi surface.

J Phys Condens Matter 2019 Apr 18. Epub 2019 Apr 18.

Department of Engineering Science, Denki Tsushin Daigaku, 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, JAPAN.

The magnetoresistance (MR) of SrTiO<sub>3</sub> is theoretically investigated based on the Boltzmann equation by considering its detailed band structure. The formula for MR proposed by Mackey and Sybert is extended to be applicable to a system with an arbitrarily shaped Fermi surface. It is shown that the angular dependence of the diagonal component of the mass tensor causes transverse MR, whereas that of the offdiagonal component causes longitudinal MR with only a single closed Fermi surface, which overturns the textbook understanding of MR. Read More

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http://dx.doi.org/10.1088/1361-648X/ab1b05DOI Listing

Perinatal breastfeeding interventions including fathers/partners: A systematic review of the literature.

Midwifery 2019 Apr 3;75:41-51. Epub 2019 Apr 3.

St. Michael's Hospital, University of Toronto, Canada.

Objective: Support from fathers is consistently associated with improved breastfeeding duration and exclusivity rates. Additionally, there is growing evidence that fathers want to be included in breastfeeding interventions provided by health care professionals. The objective of this review was to determine the effect of partner-inclusive educational and psychosocial interventions on breastfeeding initiation, duration, and exclusivity. Read More

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http://dx.doi.org/10.1016/j.midw.2019.04.001DOI Listing

Intraoperative ultrasonography (ioUS) characteristics of focal cortical dysplasia (FCD) type II b.

Seizure 2019 Mar 12;69:80-86. Epub 2019 Mar 12.

Department of Neurosurgery, Fondazione IRCCS Istituto Neurologico C. Besta, Milan, Italy; Department of Neurological Surgery, Johns Hopkins Medical School, Baltimore, MD, USA.

Purpose: Focal cortical dysplasia (FCD) is one of the major causes of drug-resistant epilepsy. Surgery has proved to be the treatment of choice, however up to a third of patients experience only partial resection. Ill-defined borders and lesions embedded in eloquent areas are two of the main drawbacks of FCD surgery. Read More

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http://dx.doi.org/10.1016/j.seizure.2019.02.020DOI Listing

Computational approaches to macromolecular interactions in the cell.

Curr Opin Struct Biol 2019 Apr 15;55:59-65. Epub 2019 Apr 15.

Computational Biology Program and Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA. Electronic address:

Structural modeling of a cell is an evolving strategic direction in computational structural biology. It takes advantage of new powerful modeling techniques, deeper understanding of fundamental principles of molecular structure and assembly, and rapid growth of the amount of structural data generated by experimental techniques. Key modeling approaches to principal types of macromolecular assemblies in a cell already exist. Read More

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http://dx.doi.org/10.1016/j.sbi.2019.03.012DOI Listing

Folding the genome into mitotic chromosomes.

Curr Opin Cell Biol 2019 Apr 15;60:19-26. Epub 2019 Apr 15.

Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan. Electronic address:

How linear DNA molecules are packaged into compact cylindrical chromosomes in preparation for cell division has remained one of the central outstanding questions in cell biology. Condensin is a highly conserved protein complex that universally determines large-scale DNA geometry during mitotic chromosome assembly. A wide range of recently developed approaches, including super resolution microscopy, single molecule imaging, Hi-C analyses and computational modeling, have profoundly changed how we view mitotic chromosomes. Read More

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http://dx.doi.org/10.1016/j.ceb.2019.03.005DOI Listing

The investigation of fluid flow in cartilage contact gap.

J Mech Behav Biomed Mater 2019 Apr 13;95:153-164. Epub 2019 Apr 13.

Department of Infrastructure Engineering, The University of Melbourne, Victoria, 3010, Australia. Electronic address:

Synovial fluid flow in articular joint capsule plays an important role during mixed mode lubrication. However, the actual fluid flow behaviour during cartilage contact has not been fully understood so far. This is due to the difficulties in measuring the gap permeability using conventional experimental techniques. Read More

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http://dx.doi.org/10.1016/j.jmbbm.2019.04.008DOI Listing

Baicalein attenuates pancreatic inflammatory injury through regulating MAPK, STAT 3 and NF-κB activation.

Int Immunopharmacol 2019 Apr 15;72:204-210. Epub 2019 Apr 15.

Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address:

Acute pancreatitis (AP) is a common acute abdominal disease with local or systemic inflammatory response, caused by abnormal activation of digestive enzymes. Baicalein has been shown to exert anti-inflammatory effects and to attenuate the pathological changes of AP. The aim of the research was to investigate the effects of baicalein on caerulein induced pancreatitis, and to elucidate the putative underlying mechanism. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.04.018DOI Listing

Experimental and theoretical insights on the effect of solvent polarity on the photophysical properties of a benzanthrone dye.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Apr 3;218:221-228. Epub 2019 Apr 3.

Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia.

Benzanthrone derivatives show interesting solvent dependent photophysical properties. Understanding of their photophysical properties is essential for developing the fluorescence probes based on benzanthrone derivatives. The photophysical properties of 3-(N'-chlorophenyl)piperazino-7H-benzo[de]anthracen-7-one [ClPh-PBA] molecule are reported in different solvents and solvent mixtures. Read More

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http://dx.doi.org/10.1016/j.saa.2019.04.001DOI Listing

Use of Pistia stratiotes for phytoremediation of water resources contaminated by clomazone.

Chemosphere 2019 Apr 5;227:299-304. Epub 2019 Apr 5.

Laboratório de Estudos Físico-Químicos e de Produtos Naturais (LEFQPN), Campus Uruguaiana, Universidade Federal do Pampa, CEP 97508-000, Uruguaiana, RS, Brazil. Electronic address:

The Pistia stratiotes L. was tested for phytoremediation potential of the compound clomazone in water. Clomazone is a post-emergent herbicide marketed as Gamit. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.04.013DOI Listing

Photobiomodulation associated to cellular therapy improve wound healing of experimental full thickness burn wounds in rats.

J Photochem Photobiol B 2019 Apr 10;194:174-182. Epub 2019 Apr 10.

Laboratory of Experimental Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil. Electronic address:

Adipose derived stromal vascular fraction (SVF) is a method of cell therapy potentially applicable for treatment of full thickness burns. Here we investigated if the association of photobiomodulation (PBM) with SVF therapy could improve wound healing in experimentally induced full thickness burn wounds in rats compared to the topical agent 2% silver sulfadiazine in a dose-dependent manner. Sixty-six male Wistar rats were divided in 4 groups containing 5 animals each which received the following treatments: 2% sulfadiazine (SD), SVF, SVF plus PBM at 30 mW (SVFL30), and SVF plus PBM at 100 mW (SVFL100). Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.04.003DOI Listing

Bone char as a green sorbent for removing health threatening fluoride from drinking water.

Environ Int 2019 Apr 15;127:704-719. Epub 2019 Apr 15.

Research and Engineering Services, Toowoomba, QLD, 4350, Australia.

Millions of people around the world suffer from or prone to health problems caused by high concentration of fluoride in drinking water sources. One of the environmentally friendly and cost-effective ways for removing fluoride is the use of bone char. In this review, the structural properties and binding affinity of fluoride ions from different water sources was critically discussed. Read More

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http://dx.doi.org/10.1016/j.envint.2019.03.065DOI Listing

Multicenter evaluation of a novel ROS1 immunohistochemistry assay (SP384) for detection of ROS1 rearrangements in a large cohort of lung adenocarcinoma patients.

J Thorac Oncol 2019 Apr 15. Epub 2019 Apr 15.

Université Côte d'Azur, University Hospital Federation OncoAge, Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Nice, France;; Université Côte d'Azur, CNRS, INSERM, Institute of Research on Cancer and Ageing of Nice (IRCAN), University Hospital Federation OncoAge, Nice, France;; Université Côte d'Azur, University Hospital Federation OncoAge, Hospital-Related Biobank (BB-0033-00025), Pasteur Hospital, Nice, France;. Electronic address:

Introduction: The detection of a ROS1 rearrangement in advanced and metastatic lung adenocarcinoma (LUAD) lead to a targeted treatment with tyrosine kinase inhibitors, with favorable progression-free survival and overall survival of the patients. Thus, it is mandatory to screen for the ROS1 rearrangement in all these patients. ROS1 rearrangements can be detected using break-apart fluorescence in situ hybridization (FISH), which is the "gold standard"; however, ROS1 immunohistochemistry (IHC) can be used as a screening test since it is widely available, easy and rapid to perform, and cost-effective. Read More

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http://dx.doi.org/10.1016/j.jtho.2019.03.024DOI Listing

Molecular regulation of myoendothelial gap junctions.

Curr Opin Pharmacol 2019 Apr 15;45:16-22. Epub 2019 Apr 15.

Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, Biomedical Centre, Cardiovascular Physiology, LMU Munich and German Centre for Cardiovascular Research, Partner Site Munich Heart Alliance, Munich, Germany.

Myoendothelial gap junctions are involved in the regulation of vascular tone. The major connexins described in the vascular system are Cx37, Cx40, Cx43, and Cx45 with all but Cx45 found in myoendothelial connections. Although many reports on post-translational modifications of these connexins are available, only few groups have investigated their role in controlling myoendothelial communication and signal propagation. Read More

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http://dx.doi.org/10.1016/j.coph.2019.03.006DOI Listing

The effect of adjunctive nutraceuticals in bipolar disorder: A systematic review of randomized placebo-controlled trials.

J Affect Disord 2019 Apr 8;252:334-349. Epub 2019 Apr 8.

Department of Clinical and Experimental Medicine, Psychiatry Unit, University of Catania, Italy; U.O.C. Clinica Psichiatrica, A.O.U. Policlinico-Vittorio Emanuele, Presidio "G. Rodolico", Catania, Italy.

Background: Nutraceuticals are a group of compounds of growing interest for mental health professionals. Given the implication of certain nutrients in the onset of bipolar disorder, it has been hypothesized that nutraceuticals might be effective in improving symptoms of the condition (i.e. Read More

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http://dx.doi.org/10.1016/j.jad.2019.04.039DOI Listing

Finally an evidence-based indication for injectable phloroglucinol!

J Gynecol Obstet Hum Reprod 2019 Apr 15. Epub 2019 Apr 15.

CHU Toulouse, Pôle de Gynécologie Obstétrique, Hôpital Paule de Viguier, Toulouse, F-31059, France; Université de Toulouse III, UMR1027, Toulouse, F-31073, France. Electronic address:

Background: While phloroglucinol is widely prescribed in European countries for its antispasmodic properties, recent high quality data failed to demonstrate its superiority to placebo in alleviating abdominal pain. Rumors suggest that injectable presentation of phloroglucinol may erase povidone-iodine stains. We thus aimed to evaluate its efficacy in this new indication. Read More

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http://dx.doi.org/10.1016/j.jogoh.2019.04.008DOI Listing

Genome scale metabolic modeling reveals the metabolic potential of three Type II methanotrophs of the genus Methylocystis.

Metab Eng 2019 Apr 15. Epub 2019 Apr 15.

Departamento de Ingeniería Química y Tecnología del medio ambiente, Escuela de Ingenierías Industriales, Universidad de Valladolid, Spain; Institute of Sustainable Processes, Universidad de Valladolid, Spain.

Genome Scale Metabolic Models (GSMMs) of the recently sequenced Methylocystis hirsuta and two other methanotrophs from the genus Methylocystis have been reconstructed. These organisms are Type II methanotrophs with the ability of accumulating Polyhydroxyalkanoates under nutrient limiting conditions. For the first time, GSMMs have been reconstructed for Type II methanotrophs. Read More

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http://dx.doi.org/10.1016/j.ymben.2019.04.001DOI Listing

Inhalable co-amorphous budesonide-arginine dry powders prepared by spray drying.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Department of Pharmacy, University of Copenhagen, Universitetsparken 2, DK-2100, Copenhagen, Denmark; Wuya College of Innovation, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, China. Electronic address:

Spray drying is a well-established technology to produce inhalable dry powders. However, the amorphous nature of the particles typically obtained from the process can lead to physically and chemically unstable products. The purpose of this study was to investigate whether spray-drying could be used as a manufacturing method to produce co-amorphous drug amino acid powders with high physical stability and inhalable particulate properties. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03785173193029
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http://dx.doi.org/10.1016/j.ijpharm.2019.04.036DOI Listing
April 2019
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Development and pharmacokinetics of a combination vaginal ring for sustained release of dapivirine and the protein microbicide 5P12-RANTES.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. Electronic address:

The past fifteen years have witnessed a resurgence of interest in vaginal ring technologies for drug delivery applications, mostly driven by the impetus for development of vaginally-administered antiretroviral microbicides to help reduce the high acquisition rates for human immunodeficiency virus (HIV) among Sub-Saharan African women. Currently, the lead candidate microbicide is a 28-day silicone elastomer vaginal ring releasing dapivirine (Ring-004), an experimental non-nucleoside reverse transcriptase inhibitor. The ring was tested in two pivotal Phase III clinical studies in 2016 and is currently undergoing review by the European Medicines Agency. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.040DOI Listing

Functional Connectivity Pattern in the Core Face Network Reflects Different Mechanisms of Holistic Face Processing Measured by the Whole-Part Effect and Composite-Face Effect.

Neuroscience 2019 Apr 15. Epub 2019 Apr 15.

Beijing Key Laboratory of Applied Experimental Psychology, Faculty of Psychology, Beijing Normal University, Beijing 100875, China.

Holistic face processing is a critical component of face recognition. There are two classical measures of holistic face processing: the whole-part effect (WPE) and composite-face effect (CFE). However, the two effects have demonstrated inconsistent pattern of results in behavioral literature. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.04.017DOI Listing

Intravenous Infusion of Mesenchymal Stem Cells Improves Impaired Cognitive Function in a Cerebral Small Vessel Disease Model.

Neuroscience 2019 Apr 15. Epub 2019 Apr 15.

Department of Neural Regenerative Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan; Department of Neurology, Yale University School of Medicine, New Haven, CT, 06510, USA; Center for Neuroscience and Regeneration Research, VA Connecticut Healthcare System, West Haven, CT, 06516, USA.

Cerebral small vessel disease (CSVD) is not only a cause of vascular dementia (VD) but also a contributing factor to Alzheimer's disease (AD). The essential pathological feature of CSVD is the disruption of blood-brain barrier (BBB). Dysfunction of BBB due to degeneration of both endothelial cells and pericytes in capillaries leads to neuronal damage and progressive brain atrophy. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.04.018DOI Listing

Four limonoids from Bacillus subtilis-fermented neem seeds and their cytotoxic activity.

Fitoterapia 2019 Apr 15. Epub 2019 Apr 15.

College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address:

Four new limonoids, 7,12-dihydroxyvilasinone (1), vilasindione (2), 4-dehydroxynimbandiol (3) and azadiramide B (4), were isolated from extracts of Bacillus subtilis-fermented neem seeds. Their planar structures and relative configurations were elucidated by spectroscopic methods including UV, IR, MS and NMR, and the absolute stereochemistry was determined by comparing their experimental and calculated CD spectra. 4 is a rare salannin-class limonoid alkaloid. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.04.004DOI Listing

The key role of the elongation factors in the origin of the organization of the genetic code.

Biosystems 2019 Apr 15. Epub 2019 Apr 15.

The Ionian School, Genetic Code and tRNA Origin Laboratory, Via Roma 19, 67030, Alfedena, L'Aquila, Italy; Institute of Biosciences and Bioresources, CNR, Via P. Castellino, 111, 80131, Naples, Italy. Electronic address:

I suggest a model based on primordial elongation factors able to explain a relevant aspect of the organization of the code itself: why the biosynthetic relationships between amino acids are mainly allocated on the rows of the genetic code. I hypothesize the existence of specific primordial elongation factors able to recognize two aspects of the ancestral aminoacylated-tRNA: the third base of the anticodon and a distinctive recognition sequence, i.e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03032647193009
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http://dx.doi.org/10.1016/j.biosystems.2019.04.009DOI Listing
April 2019
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Differential Expression of HMGA1 and HMGA2 in pituitary neuroendocrine tumors.

Mol Cell Endocrinol 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Experimental Endocrinology - LEEx, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil; Graduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Brazil; Graduate Program in Pharmacology and Medicinal Chemistry, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil. Electronic address:

Defining biomarkers for invasive pituitary neuroendocrine tumors (PitNETs) is highly desirable. The high mobility group A (HMGA) proteins are among the most widely expressed cancer-associated proteins. Indeed, their overexpression is a frequent feature of human malignancies, including PitNETs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193011
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http://dx.doi.org/10.1016/j.mce.2019.04.010DOI Listing
April 2019
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Age, but not severity of injury, mediates decline in executive function: Validation of the rodent touchscreen paradigm for preclinical models of traumatic brain injury.

Behav Brain Res 2019 Apr 15:111912. Epub 2019 Apr 15.

Cognition, Ageing and Neurodegenerative Disease Lab, Adelaide Medical School, The University of Adelaide, Adelaide, SA 5005 Australia. Electronic address:

Increasingly, it is being recognised that traumatic brain injury (TBI) is not just an acute event but instead results in ongoing neuronal injury that may lead to chronic impairments in multiple cognitive domains. Of these, deficits in executive function are one of the more common changes reported following TBI, and are a major predictor of well-being, social function and quality of life in individuals with a history of TBI. In order to fully understand the relationship between TBI and executive dysfunction, including brain mechanisms that may account for this, experimental models are clearly needed. Read More

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http://dx.doi.org/10.1016/j.bbr.2019.111912DOI Listing

IL-17-high asthma with features of a psoriasis immunophenotype.

J Allergy Clin Immunol 2019 Apr 15. Epub 2019 Apr 15.

Respiratory, Inflammation, Autoimmunity IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden.

Background: The role of interleukin-17 immunity is well established in inflammatory diseases like psoriasis and inflammatory bowel disease but not in asthma where further study is required.

Objective: To undertake a deep-phenotyping study of asthmatics with up-regulated interleukin-17 immunity.

Methods: Whole genome transcriptomic analysis was performed using epithelial brushings, bronchial biopsies (91 asthmatics patients and 46 healthy controls) and whole blood samples (n=498) from the U-BIOPRED cohort. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00916749193048
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http://dx.doi.org/10.1016/j.jaci.2019.03.027DOI Listing
April 2019
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Gene editing-mediated disruption of epidermolytic ichthyosis-associated KRT10 alleles restores filament stability in keratinocytes.

J Invest Dermatol 2019 Apr 15. Epub 2019 Apr 15.

EB House Austria, Research Program for Molecular Therapy of Genodermatoses, Department of Dermatology, University Hospital of the Paracelsus Medical University Salzburg, Austria. Electronic address:

Epidermolytic ichthyosis (EI) is a skin fragility disorder caused by dominant-negative mutations in KRT1 or KRT10. No definitive restorative therapies exist that target these genetic faults. Gene editing can be used to efficiently introduce frameshift mutations to inactivate mutant genes. Read More

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http://dx.doi.org/10.1016/j.jid.2019.03.1146DOI Listing

Activation of the PP2A catalytic subunit by ivabradine attenuates the development of diabetic cardiomyopathy.

J Mol Cell Cardiol 2019 Apr 15. Epub 2019 Apr 15.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China. Electronic address:

Hyperglycemia-induced apoptosis plays a critical role in the pathogenesis of diabetic cardiomyopathy (DCM). Our previous study demonstrated that ivabradine, a selective I current antagonist, significantly attenuated myocardial apoptosis in diabetic mice, but the underlying mechanisms remained unknown. This study investigated the underlying mechanisms by which ivabradine exerts anti-apoptotic effects in experimental DCM. Read More

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http://dx.doi.org/10.1016/j.yjmcc.2019.04.011DOI Listing

Erythropoietin does not improve fracture healing in aged mice.

Exp Gerontol 2019 Apr 15. Epub 2019 Apr 15.

Department of Trauma, Hand and Reconstructive Surgery, Saarland University, 66421 Homburg, Germany; Institute for Clinical & Experimental Surgery, Saarland University, 66421 Homburg, Germany.

Fracture healing in the elderly is associated with a declined healing potential caused by multiple factors including a delay of vascularization. Erythropoietin (EPO) has been demonstrated to improve vascularization and fracture healing in adult mice. We, therefore, hypothesized that EPO in aged mice also improves fracture healing. Read More

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http://dx.doi.org/10.1016/j.exger.2019.04.005DOI Listing

Leishmania infantum LeIF and its recombinant polypeptides induce the maturation of dendritic cells in vitro: an insight for dendritic cells based vaccine.

Immunol Lett 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Molecular Epidemiology and Experimental Pathology, Institut Pasteur de Tunis, Université Tunis El Manar, 13 Place Pasteur, BP 74, 1002 Tunis-Belvedère, Tunisia. Electronic address:

We previously showed that recombinant Leishmania infantum eukaryotic initiation factor (LieIF) was able to induce the secretion of cytokines IL-12, IL-10 and TNF-α by human monocytes. In this study, we explored in vitro the potential of LieIF to induce phenotypic maturation and functional differentiation of murine bone - marrow derived dendritic cells (BM-DCs). Moreover, in order to identify potential immunnomodulatory regions of LieIF, eight recombinant overlapping protein fragments covering the whole amino acid sequence ofprotein; were constructed and assessed in vitro for their ability to induce maturation of BM-DCs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01652478183041
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http://dx.doi.org/10.1016/j.imlet.2019.04.001DOI Listing
April 2019
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Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity.

J Dent 2019 Apr 15. Epub 2019 Apr 15.

Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy. Electronic address:

Objective: This in vitro study evaluated at baseline (T0) and over time (T12 months), the effect of a multi-mode universal adhesive compared with two experimental formulations blended with different concentrations of benzalkonium chloride (BAC), on bond strength and endogenous enzymatic activity.

Methods And Materials: Specimens were assigned to the following groups according to the adhesive protocol: G1) All-Bond Universal (ABU) self-etch (SE); G2) ABU + 0.5% BAC SE; G3) ABU + 1% methacrylate BAC SE; G4) ABU etch-and-rinse (E&R); G5) ABU + 0. Read More

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http://dx.doi.org/10.1016/j.jdent.2019.04.008DOI Listing

Epigallocatechin-3-gallate stimulates autophagy and reduces apoptosis levels in retinal Müller cells under high-glucose conditions.

Exp Cell Res 2019 Apr 15. Epub 2019 Apr 15.

Department of Ophthalmology, Lixiang Eye Hospital of Soochow University, Suzhou, 215021, China. Electronic address:

Retinal neurodegeneration is an early feature in the pathogenesis of diabetic retinopathy (DR). Autophagy is an intracellular catabolic process involved in protein and organelle degradation that has been linked in DR. Epigallocatechin gallate (EGCG) is a major polyphenol in green tea that has beneficial effects in diabetic. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.014DOI Listing

Mesenchymal Stem Cells: from Regeneration to Cancer.

Pharmacol Ther 2019 Apr 15. Epub 2019 Apr 15.

The Jackson Laboratory, Bar Harbor, ME 04609, USA. Electronic address:

Mesenchymal stem cells (MSCs) are multipotent tissue stem cells that differentiate into a number of mesodermal tissue types, including osteoblasts, adipocytes, chondrocytes and myofibroblasts. MSCs were originally identified in the bone marrow (BM) of humans and other mammals, but recent studies have shown that they are multilineage progenitors in various adult organs and tissues. MSCs that localize at perivascular sites function to rapidly respond to external stimuli and coordinate with the vascular and immune systems to accomplish the wound healing process. Read More

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http://dx.doi.org/10.1016/j.pharmthera.2019.04.005DOI Listing

Omega-3 fatty acids protects against chronic sleep-deprivation induced memory impairment.

Life Sci 2019 Apr 15. Epub 2019 Apr 15.

Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.

Aims: The current study aims to evaluate the possible protective effect of omega-3 fatty acids on memory impairment induced by sleep-deprivation in rats.

Materials And Methods: Animals were chronically sleep deprived using the modified multiple platform model (8 h/day for 8 weeks). Omega-3 fatty acids were administered as fish oil via oral gavage at a daily dose of 100 mg omega-3 PUFA/100 g BWT. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.04.028DOI Listing

Cadmium exposure upregulates SNAIL through miR-30 repression in human lung epithelial cells.

Toxicol Appl Pharmacol 2019 Apr 15. Epub 2019 Apr 15.

Department of Environmental Medicine, New York University School of Medicine, New York, NY 10010, USA. Electronic address:

Cadmium (Cd) is a known human lung carcinogen. In addition, Cd exposure is associated with several lung diseases including emphysema, chronic obstructive pulmonary disease (COPD), asthma and fibrosis. Although earlier studies have identified several processes dysregulated by Cd exposure, the underlying mechanisms remain unclear. Read More

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http://dx.doi.org/10.1016/j.taap.2019.04.011DOI Listing

The formation of the Thickveins (Tkv) gradient in Drosophila wing discs: A theoretical study.

Authors:
Zhan Chen

J Theor Biol 2019 Apr 15. Epub 2019 Apr 15.

Department of Mathematical Sciences, Georgia Southern University, Statesboro, GA, United States.

The development of wing imaginal disc (wing disc) is commonly adopted for the studies of patterning and growth which are two fundamental problems in developmental biology. Decapentaplegic (Dpp) signaling regulates several aspects of wing development, such as the anterior (A)-posterior (P) patterning, cellular growth rate, and cell adhesion. The distribution and activity of Dpp signaling are controlled in part by the expression level of its major type I receptor, Thickveins (Tkv). Read More

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http://dx.doi.org/10.1016/j.jtbi.2019.04.015DOI Listing

Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain.

J Mol Biol 2019 Apr 15. Epub 2019 Apr 15.

Department of Pharmacy, University of Patras, GR-26504, Patras, Greece. Electronic address:

Mayaro virus (MAYV) is a member of Togaviridae family which also includes Chikungunya virus as a notorious member. MAYV recently emerged in urban areas of the Americas, and this emergence emphasized the current paucity of knowledge about its replication cycle. The macro domain (MD) of MAYV belongs to the N-terminal region of its non-structural protein 3, part of the replication complex. Read More

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http://dx.doi.org/10.1016/j.jmb.2019.04.013DOI Listing

Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.

Arch Biochem Biophys 2019 Apr 15. Epub 2019 Apr 15.

Nanoscience & Nanotechnology & Innovative Instrumentation (NAST) Centre, University of Rome Tor Vergata, Rome, Italy; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, USA. Electronic address:

Matrix vesicles (MVs) are a class of extracellular vesicles that initiate mineralization in cartilage, bone, and other vertebrate tissues by accumulating calcium ions (Ca) and inorganic phosphate (P) within their lumen and forming a nucleation core (NC). After further sequestration of Ca and P, the NC transforms into crystalline complexes. Direct evidence of the existence of the NC and its maturation have been provided solely by analyses of dried samples. Read More

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http://dx.doi.org/10.1016/j.abb.2019.04.003DOI Listing

Automated in vivo screen in zebrafish identifies clotrimazole as targeting a metabolic vulnerability in a melanoma model.

Dev Biol 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Experimental Cancer Biology, CIBIO Department, University of Trento, Via Sommarive 9, 38123 Povo TN, Italy. Electronic address:

Therapeutic approaches for cutaneous melanoma are flourishing, but despite promising results, there is an increasing number of reported primary or secondary resistance to the growing sets of drugs approved for therapy in the clinics. Combinatorial approaches may overcome resistance, as they may tackle specific weaknesses of melanoma cells, not sufficient on their own, but effective in combination with other therapies. The transgenic zebrafish line kita:ras develops melanoma with high frequency. Read More

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http://dx.doi.org/10.1016/j.ydbio.2019.04.005DOI Listing

Enhancing the training of trauma resuscitation flash teams: A mixed methods study.

Australas Emerg Care 2018 Nov 29;21(4):143-149. Epub 2018 Oct 29.

Sydney Nursing School, University of Sydney, Sydney, NSW, Australia; Emergency Department, Illawarra Shoalhaven, Australia.

Aims: To determine whether simulated multidisciplinary team training influences teamwork practices and experiences when resuscitating critically injured patients and to generate evidence for training trauma resuscitation flash teams.

Background: Trauma teams perform in stressful situations. They are 'flash' teams, mobilised quickly and comprise of different specialties and disciplines. Read More

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http://dx.doi.org/10.1016/j.auec.2018.10.002DOI Listing
November 2018

Bioelectrical Impedance Analysis Does Not Detect an Increase in Total Body Water Following Isotonic Fluid Consumption.

Appl Physiol Nutr Metab 2019 Apr 18. Epub 2019 Apr 18.

Montclair State University, Exercise Science and Physical Education , 1 Normal Avenue , University Hall Suite 4136 , Montclair, New Jersey, United States , 07043 ;

Purpose: To determine if single frequency foot-to-foot bioelectrical impedance analysis (BIA) can detect acute changes in total body water (TBW) following consumption of isotonic saline.

Methods: All participants ate a sodium free meal 4hr prior to the data collection visit and had euhydration confirmed using urine specific gravity at the beginning of the experimental visit. Subjects drank 466mL of isotonic saline (Na+ 140mmol∙L-1) following baseline measures. Read More

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http://dx.doi.org/10.1139/apnm-2019-0106DOI Listing

Feedforward Self-Modeling and Self-Regulation: It's Not Just for Learning.

Res Q Exerc Sport 2019 Apr 18:1-11. Epub 2019 Apr 18.

b University of Ottawa.

Purpose: This research investigated whether Zimmerman's model of self-regulated learning could be transferred into a competitive setting. We also investigated whether a feedforward self-modeling video could influence one's use of self-regulation and as such be an effective self-regulatory intervention.

Methods: Nine competitive gymnasts took part in two interviews; one interview was conducted after a competition in which the video was viewed (experimental interview: EI) and the other after a competition where no video was seen (control interview: CI). Read More

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http://dx.doi.org/10.1080/02701367.2019.1593923DOI Listing

Exploring the Importance of Surface Diffusion in Stability of Vapor-Deposited Organic Glasses.

J Phys Chem B 2019 Apr 18. Epub 2019 Apr 18.

Stable glasses are formed during physical vapor deposition (PDV), through the surface-mediated equilibration process. Understanding surface relaxation dynamics is important in understanding the details of this process. Direct measurements of the surface relaxation times in molecular glass systems are challenging. Read More

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http://dx.doi.org/10.1021/acs.jpcb.9b01012DOI Listing

Transition-Metal Free Deconstructive Lactamization of Piperidines.

Angew Chem Int Ed Engl 2019 Apr 18. Epub 2019 Apr 18.

MEXICO.

One of the major synthetic challenges in organic synthesis is the activation or deconstructive functionalization of unreactive C(sp3)-C(sp3) bonds. This requires using transition or precious metal catalysts. However, we present here a deconstructive lactamization of piperidines without using transition metal catalysts. Read More

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http://dx.doi.org/10.1002/anie.201903880DOI Listing

Association of Exercise and Swimming Goggles With Modulation of Cerebro-ocular Hemodynamics and Pressures in a Model of Spaceflight-Associated Neuro-ocular Syndrome.

JAMA Ophthalmol 2019 Apr 18. Epub 2019 Apr 18.

Integrated Cardiovascular Exercise Physiology and Rehabilitation Laboratory, College of Nursing and Health Innovation, University of Texas at Arlington, Arlington.

Importance: Astronauts on International Space Station missions demonstrate adverse neuro-ocular changes. Reversing a negative translaminar pressure gradient (TLPG) by modulating cerebral blood flow, decreasing intracranial pressure, or increasing intraocular pressure (IOP) has been proposed as potential intervention for spaceflight-associated neuro-ocular syndrome (SANS).

Objective: To examine whether exercise (resistance, moderate-intensity aerobic, and high-intensity aerobic) or artificially increasing IOP is associated with modulated cerebro-ocular hemodynamic and pressure changes during head-down tilt (HDT), an analogue of spaceflight, in healthy adults. Read More

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http://dx.doi.org/10.1001/jamaophthalmol.2019.0459DOI Listing

Reference genes selection for Calotropis procera under different salt stress conditions.

PLoS One 2019 18;14(4):e0215729. Epub 2019 Apr 18.

Universidade Federal de Pernambuco, Departamento de Botânica, Laboratório de Fisiologia Vegetal, Recife, PE, Brazil.

Calotropis procera is a perennial Asian shrub with significant adaptation to adverse climate conditions and poor soils. Given its increased salt and drought stress tolerance, C. procera stands out as a powerful candidate to provide alternative genetic resources for biotechnological approaches. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215729PLOS

TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice.

PLoS Pathog 2019 Apr 18;15(4):e1007657. Epub 2019 Apr 18.

Biomedical and Life Sciences, Faculty of Health and Medicine, University of Lancaster, Lancaster, United Kingdom.

Helminths are highly prevalent metazoan parasites that infect over a billion of the world's population. Hosts have evolved numerous mechanisms to drive the expulsion of these parasites via Th2-driven immunity, but these responses must be tightly controlled to prevent equally devastating immunopathology. However, mechanisms that regulate this balance are still unclear. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007657DOI Listing

Racial/ethnic differences in experimental pain sensitivity and associated factors - Cardiovascular responsiveness and psychological status.

PLoS One 2019 18;14(4):e0215534. Epub 2019 Apr 18.

Department of Pain and Translational Symptom Science, University of Maryland School of Nursing, Baltimore, Maryland, United States of America.

This study evaluated the contributions of psychological status and cardiovascular responsiveness to racial/ethnic differences in experimental pain sensitivity. The baseline measures of 3,159 healthy individuals-non-Hispanic white (NHW): 1,637, African-American (AA): 1,012, Asian: 299, and Hispanic: 211-from the OPPERA prospective cohort study were used. Cardiovascular responsiveness measures and psychological status were included in structural equation modeling based mediation analyses. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215534PLOS
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