4,135 results match your criteria Journal of Clinical Virology[Journal]


More targeted use of oseltamivir and in-hospital isolation facilities after implementation of a multifaceted strategy including a rapid molecular diagnostic panel for respiratory viruses in immunocompromised adult patients.

J Clin Virol 2019 Apr 7;116:11-17. Epub 2019 Apr 7.

Department of Infectious Diseases, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.

Background: Immunocompromised adults are more vulnerable to a complicated course of viral respiratory tract infections (RTI).

Objectives: Provide evidence on the effect of implementation of rapid molecular diagnostics for viruses on use of in-hospital isolation facilities, oseltamivir and antibiotic usage, and other clinical outcomes in immunocompromised patients.

Study Design: A before-after study during two consecutive respiratory viral seasons, including immunocompromised adult patients presenting at a tertiary care emergency department with clinical suspicion of RTI. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193007
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http://dx.doi.org/10.1016/j.jcv.2019.04.003DOI Listing
April 2019
1 Read

Human Herpes-8 virus copy to cell ratio: A diagnostic tool in primary effusion lymphoma.

J Clin Virol 2019 Mar 22;116:7-10. Epub 2019 Mar 22.

Virus Reference Department, National Infection Service, Public Health England, 61 Colindale Ave, London, NW9 5HT, United Kingdom. Electronic address:

Primary effusion lymphoma (PEL) is a serious sequel to Human Herpes Virus 8 (HHV8) infection in the immunosuppressed host. Usually requiring a cytological diagnosis, body cavity effusions are often referred for investigation for possible PEL. Although absence of HHV8 effectively refutes this, the presence of HHV8 DNA, though indicative is not diagnostic. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.013DOI Listing
March 2019
2 Reads

Multicenter evaluation of the cobas® HIV-1 quantitative nucleic acid test for use on the cobas® 4800 system for the quantification of HIV-1 plasma viral load.

J Clin Virol 2019 Mar 16;114:43-49. Epub 2019 Mar 16.

Department of Infection and Immunity, Luxembourg Institute of Health, Luxembourg. Electronic address:

Background And Objectives: Measurement of HIV-1 viral load (VL) is necessary to monitor treatment efficacy in patients receiving antiretroviral therapy. We evaluated the performance of the cobas® HIV-1 quantitative nucleic acid test for use on the cobas® 4800 system ("cobas 4800 HIV-1").

Methods: Limit of detection, linearity, accuracy, precision, and specificity of cobas 4800 HIV-1, COBAS® AmpliPrep/COBAS® Taqman® HIV-1 version 2. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.008DOI Listing
March 2019
2 Reads

No correlation between Torque Teno virus viral load and BK virus replication after kidney transplantation.

J Clin Virol 2019 Apr 1;116:4-6. Epub 2019 Apr 1.

Department of Virology, Amiens University Medical Center, Amiens, France; AGIR Research Unit, EA4294, Jules Verne University of Picardie, Amiens, France. Electronic address:

Background: Assessment of the intensity of immunosuppression in transplant recipients to estimate the risk of rejection and infection is not entirely satisfactory at the present time. Determination of Torque teno virus (TTV) viral load appears to be a promising tool in this setting.

Objectives: We evaluated the level of replication and kinetics of TTV during the first three months after kidney transplantation compared to BK virus replication. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.018DOI Listing

BioRad BioPlex® HIV Ag-Ab assay: Incidence of false positivity in a low-prevalence population and its effects on the current HIV testing algorithm.

J Clin Virol 2019 Apr 6;116:1-3. Epub 2019 Apr 6.

Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States; Department of Biology and Wildlife, Institute of Arctic Biology, University of Alaska -Fairbanks, Fairbanks, Alaska, United States.

Background: The BioPlex® HIV Ag-Ab assay, unlike other HIV 1/2 antigen/antibody immunoassays, is capable of differentiating positive HIV-1 antibodies (Groups M and O) from HIV-2 antibodies and/or HIV-1 p24 antigen in a single test.

Objective: The Alaska State Virology Laboratory (ASVL) adopted the BioPlex® HIV Ag-Ab assay early 2017 and can report on its performance in terms of false positivity in a low-prevalence population and its effects on the current HIV testing algorithm recommended by the Centers for Disease Control and Prevention (CDC).

Study Design: Specimens received between March 2017 and August 2018 were screened using the BioPlex® HIV Ag-Ab assay. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.04.002DOI Listing
April 2019
1 Read

Identification of influenza C virus in young South Korean children, from October 2013 to September 2016.

J Clin Virol 2019 Mar 27;115:47-52. Epub 2019 Mar 27.

Division of Infectious Diseases, Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea; Asia Pacific Influenza Institute, Korea University College of Medicine, Seoul, South Korea. Electronic address:

Background: Influenza C virus has been largely neglected, compared to influenza A orB viruses, and is not routinely tested in clinical practices. However, several studies have indicated that influenza C virus causes severe acute respiratory illness and pneumonia in all ages.

Objective: We conducted a study to identify influenza C virus among young children in South Korea. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.016DOI Listing
March 2019
5 Reads

Evaluating addition of self-collected throat swabs to nasal swabs for respiratory virus detection.

J Clin Virol 2019 Apr 4;115:43-46. Epub 2019 Apr 4.

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States; Department of Laboratory Medicine, University of Washington, United States.

Background: Early and accurate detection of respiratory viruses (RV) is important for patient management. We have previously shown that self-collected nasal swabs (NS) are feasible and as sensitive as clinician-collected nasal washes for detection of RV, but the additive benefit of self-collected throat swabs is unknown.

Objectives: To evaluate the added yield of self-collected nasal to throat swabs for detection of RV by PCR in patients with upper respiratory tract infection (URTI) symptoms. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.04.001DOI Listing
April 2019
2 Reads

CMV-specific T-cells and CD27-CD28-CD4+ T-cells for assignment of cytomegalovirus (CMV) status in adults awaiting organ transplant.

J Clin Virol 2019 Mar 25;115:37-42. Epub 2019 Mar 25.

Department of Medicine, University of Alberta, Edmonton T6G 2B7, Canada.

Background/objectives: Determination of Cytomegalovirus (CMV) status in solid organ transplant (SOT) candidates is essential to stratify risk of post-transplant CMV disease. Passive transfusion-acquired antibodies can make serologic determination of CMV status unreliable. We evaluated 3 assays, not affected by passive antibodies (PA), in assignment of CMV status: quantification of CMV-specific CD4 + T-cells (CMV-TC) and exhausted CD27-CD28- CD4 + T-cells, and detection of CMV DNA with Nucleic Acid Amplification Testing (NAAT). Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.014DOI Listing
March 2019
7 Reads
3.016 Impact Factor

Laboratory diagnosis of congenital CMV infection in newborns: Impact of pre-analytic factors.

J Clin Virol 2019 Apr 2;115:32-36. Epub 2019 Apr 2.

Institute of Medical Virology, University Hospital Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany. Electronic address:

Background: To identify infants with congenital cytomegalovirus (cCMV) saliva polymerase chain reaction (PCR) is an ideal screening method. However, there are only few data on the influence of pre-analytic factors on the analytical sensitivity of the CMV PCR.

Objectives: This study aimed to evaluate the performance of different swabbing materials, transport time and initial virus concentration regarding to the efficacy of recovery of CMV-DNA. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.017DOI Listing
April 2019
1 Read

Amplification chemistries in clinical virology.

J Clin Virol 2019 Mar 27;115:18-31. Epub 2019 Mar 27.

Luminex Corporation, Austin, TX, United States. Electronic address:

Molecular diagnostic methods have evolved and matured considerably over the last several decades and are constantly being evaluated and adopted by clinical laboratories for the identification of infectious pathogens. Advancement in other technologies such as fluorescence, electronics, instrumentation, automation, and sensors have made the overall diagnostic process more accurate, sensitive, and rapid. Nucleic acid based detection procedures, which rely on the fundamental principles of DNA replication have emerged as a popular and standard diagnostic method, and several commercial assays are currently available based on different nucleic acid amplification techniques. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.015DOI Listing

Rhinovirus infections in infants suggest that early detection can prevent unnecessary treatment.

J Clin Virol 2019 Mar 21;115:11-17. Epub 2019 Mar 21.

Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Ramat-Gan, 52621, Israel; Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel. Electronic address:

Background: Human rhinoviruses (hRV) are small, RNA viruses of the Picornaviridae family, which are divided into three subtypes (A, B, C). hRVs are among the most common causes for acute respiratory illnesses (ARI) involving both the upper and lower respiratory tract.

Objectives: This study aimed to assess the magnitude and characteristics of hRV infections in hospitalized children, aged less than 5 years, hospitalized in Israel during 2011-2012. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.012DOI Listing
March 2019
3.016 Impact Factor

Emerging arboviruses of medical importance in the Mediterranean region.

Authors:
Anna Papa

J Clin Virol 2019 Mar 16;115:5-10. Epub 2019 Mar 16.

Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece. Electronic address:

The epidemiology of viral infections transmitted by arthropods is changing due to a variety of parameters related to the virus, the host and the environment. The Mediterranean region is highly affected by changes in the intensity and extension of global-scale climate patterns, and, due to its location, it provides a vulnerable environment for emergence of arboviral diseases. The main arboviruses that pose currently a public health threat in the Mediterranean region are West Nile virus and Crimean-Congo hemorrhagic fever virus and, in less extend, tick-borne encephalitis virus. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.007DOI Listing
March 2019
3 Reads

Hepatitis A-2017 an unusual year in Scotland.

J Clin Virol 2019 Mar 23;115:1-4. Epub 2019 Mar 23.

West of Scotland Specialist Virology Centre, Level 5, New Lister Building, 10-16 Alexandra Parade, Glasgow, G31 2ER, United Kingdom.

Background: The number of cases of acute hepatitis A reported in Scotland each year is small, and the majority of cases have been associated with travel to endemic regions. However, in early 2017, in the midst of ongoing outbreaks of hepatitis A among MSM in Europe, there was a sharp rise in the number of cases reported to Health Protection Scotland.

Objectives: The initial aim of this study was to investigate the reason for the observed increase in cases of hepatitis A at the start of 2017. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.011DOI Listing
March 2019
2 Reads

Evaluation of nasal levels of interferon and clinical severity of influenza in children.

J Clin Virol 2019 Mar 14;114:37-42. Epub 2019 Mar 14.

Centro Infant, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil. Electronic address:

Background: Experimental data show that type I interferon has a key role in innate immune response against influenza infection.

Objective: We compared nasal levels of interferon-α2 and β among inpatients and outpatients with influenza.

Study Design: Children younger than 5 years of age with influenza-like illness seeking care at the emergency department within the first 72 h of disease onset were prospectively included. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.02.003DOI Listing
March 2019
8 Reads

Liquid biopsy of HPV DNA in cervical cancer.

J Clin Virol 2019 Mar 12;114:32-36. Epub 2019 Mar 12.

Department of Microbiology, The Chinese University of Hong Kong, Hong Kong. Electronic address:

Background: A blood test to serve as a tumor marker for cervical cancer would be useful to clinicians to guide treatment and provide an early signal for recurrence. The development of droplet digital PCR has enabled the detection of HPV DNA in patient serum, providing a potential marker for cervical cancer.

Objectives: To report on a blood-based test for HPV-specific E7 and L1 genes, which may serve as a tumor marker to guide treatment and detect early recurrence in cervical cancer. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.005DOI Listing
March 2019
2 Reads
3.016 Impact Factor

Development, validation and clinical evaluation of a broad-range pan-filovirus RT-qPCR.

J Clin Virol 2019 Mar 19;114:26-31. Epub 2019 Mar 19.

Helsinki University and Helsinki University Hospital (HUSLAB), Department of Virology, Finland; University of Helsinki, Department of Virology, Helsinki, Finland; Faculty of Veterinary Medicine, Department of Veterinary Biosciences, University of Helsinki, Finland.

Background: During the five decades since their discovery, filoviruses of four species have caused human hemorrhagic fever outbreaks: Marburg (MARV) marburgvirus, and Zaire (EBOV), Sudan (SUDV) and Bundybugyo (BDBV) ebolaviruses. The largest, devastating EBOV epidemic in West Africa in 2014-16, has been followed by outbreaks of MARV in Uganda, 2017, and EBOV in Democratic Republic of Congo, 2018, emphasizing the need to develop preparedness to diagnose all filoviruses.

Objectives: The aim of this study was to optimize a new filovirus RT-qPCR to detect all filoviruses, define its limits of detection (LOD) and perform a field evaluation with outbreak samples. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.010DOI Listing
March 2019
1 Read

Early infant diagnosis HIV-1 PCR cycle-threshold predicts infant viral load at birth.

J Clin Virol 2019 Mar 18;114:21-25. Epub 2019 Mar 18.

Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa; Empilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Department of Paediatrics and Child Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Background: HIV-1 viral load (VL) has been found to be an independent predictor for disease progression among untreated HIV-infected children. However, qualitative polymerase chain reaction (PCR) assays are routinely used for early infant diagnosis (EID).

Objectives: To predict HIV-1 VL at birth using qualitative EID real-time PCR cycle-threshold (Ct) values. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193006
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http://dx.doi.org/10.1016/j.jcv.2019.03.009DOI Listing
March 2019
8 Reads

The risk of maternal parvovirus B19 infection during pregnancy on fetal loss and fetal hydrops: A systematic review and meta-analysis.

J Clin Virol 2019 Mar 8;114:12-20. Epub 2019 Mar 8.

Chinese Evidence-based Medicine Centre and CREAT Group, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. Electronic address:

Background: Human parvovirus B19 (B19) is widespread infection in humans, yet the impact on adverse pregnancy outcomes is controversial.

Objective: to evaluate the impact of B19 infection during pregnancy on adverse pregnancy outcome, and investigated the incidence of fetal loss and fetal hydrops after maternal B19 infection during pregnancy.

Study Design: A systematic literature search was performed using Embase, Medline, PubMed, Web of science, and the Cochrane Library database for relevant publications up to 10 August 2018. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.004DOI Listing
March 2019
1 Read

Prevalence and genetic diversity of viral gastroenteritis viruses in children younger than 5 years of age in Guatemala, 2014-2015.

J Clin Virol 2019 Mar 9;114:6-11. Epub 2019 Mar 9.

Division of Viral Diseases, US Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address:

Background: Acute diarrhea is an important cause of morbidity and mortality in children and is associated with approximately 500,000 deaths/year globally. Rotavirus and norovirus are leading causes of acute diarrhea accounting for more than half of this burden.

Objective/study Design: To determine the prevalence and genotype distribution of acute diarrhea caused by rotavirus, norovirus, sapovirus and astrovirus among children <5 years of age at two departments in Guatemala from January 2014 to December 2015, we tested 471 stool specimens (202 samples from hospitalized children and 269 samples from children in ambulatory clinics) by real-time reverse transcription-PCR and genotyped positive samples. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.006DOI Listing
March 2019
2 Reads

Evaluation of four commercial extraction-quantification systems to monitor EBV or CMV viral load in whole blood.

J Clin Virol 2019 Apr 2;113:39-44. Epub 2019 Mar 2.

CHU de Rouen, Laboratoire de Virologie, F-76000 Rouen, France.

Background: Measurement of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) viral loads is commonly used to monitor posttransplant patients. Two new systems (eMAG/eSTREAM and Versant/kPCR) have been recently commercialized.

Objectives: To evaluate the performance of four systems to quantify CMV and EBV in whole blood. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193004
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http://dx.doi.org/10.1016/j.jcv.2019.03.001DOI Listing
April 2019
3 Reads

A comparison of the Quidel Solana HSV 1 + 2/VZV Assay, the Focus Diagnostics Simplexa HSV 1 & 2 Direct Assay and the Luminex Aries HSV 1&2 Assay for detection of herpes simplex virus 1 and 2 from swab specimens.

J Clin Virol 2019 Apr 2;113:35-38. Epub 2019 Mar 2.

Eastern Ontario Regional Laboratory Association, Canada; The Ottawa Hospital/The Ottawa Hospital Research Institute, Canada. Electronic address:

Background: Molecular methods enable more rapid and sensitive detection of herpes simplex virus (HSV) than viral culture.

Objective: Three commercial molecular methods, all of which detect both HSV-1 and HSV-2, were compared to viral culture for the detection of HSV from swab specimens.

Study Design: Pediatric and adult patient viral swab specimens were cultured for HSV. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.002DOI Listing
April 2019
2 Reads

Diagnostic and analytical performance of the hepatitis B core related antigen immunoassay in hepatitis B patients.

J Clin Virol 2019 Mar 4;114:1-5. Epub 2019 Mar 4.

Department of Viroscience, Erasmus MC, University Medical Center Rotterdam, the Netherlands. Electronic address:

Background: Novel serological markers for Hepatitis B virus (HBV) infection are needed for prognosis and guidance of therapy.

Objective: We evaluated the diagnostic performance of the Fujirebio Lumipulse G HBcrAg immunoassay on the Fujirebio LUMIPULSE G1200 analyzer.

Study Design: Analytical performance was examined using three HBeAg positive HBV samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193004
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http://dx.doi.org/10.1016/j.jcv.2019.03.003DOI Listing
March 2019
6 Reads

Risk stratification of HIV infection for patients needing molecular confirmation with the Abbott 4th generation Architect System.

J Clin Virol 2019 Apr 22;113:31-34. Epub 2019 Feb 22.

Homestead Hospital, Homestead, FL, United States. Electronic address:

Background: Some patients need their 4 generation HIV testing results confirmed with molecular testing after primary confirmatory testing which may not be immediately available. Further risk stratification of these patients pending the results of molecular testing may be of value not only for patient counseling but also for treatment of women in labor.

Objectives: To determine the risk of a positive test result on molecular testing for these patients. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.02.004DOI Listing
April 2019
1 Read

Undetected Chikungunya virus co-infections in a Brazilian region presenting hyper-endemic circulation of Dengue and Zika.

J Clin Virol 2019 Apr 27;113:27-30. Epub 2019 Feb 27.

Departmento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Centro de Tecnologia de Vacinas, Belo Horizonte, Brazil. Electronic address:

Background: Chikungunya virus (CHIKV) causes a disease characterized by acute onset of fever accompanied by arthralgia. Clinical similarities and co-circulation of other arboviruses such as Dengue virus (DENV) and Zika virus (ZIKV), have complicated their differentiation, making their diagnoses a challenge for the health authorities. Misdiagnosis is a serious issue to the management of patients and development of public health measures. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.02.006DOI Listing

Continued biennial circulation of enterovirus D68 in Colorado.

J Clin Virol 2019 Apr 30;113:24-26. Epub 2019 Jan 30.

Children's Hospital Colorado, Aurora, CO, USA; University of Colorado Denver, Aurora, CO, USA. Electronic address:

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http://dx.doi.org/10.1016/j.jcv.2019.01.008DOI Listing
April 2019
6 Reads

Performance of a rapid diagnostic test for screening of hepatitis C in a real-life prison setting.

J Clin Virol 2019 Apr 25;113:20-23. Epub 2019 Feb 25.

Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Hepatitis C virus (HCV) point-of-care testing using rapid diagnostic test (RDT) is the solution for large-scale, feasible, fast and reliable screening of HCV infection.

Objectives: The aim of this study was to evaluate the diagnostic performance of HCV RDT for screening of HCV infection in a real-life prison setting.

Study Design: This study was conducted on individuals admitted and incarcerated in the Central Prison of Karaj, 2017-2018. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193002
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http://dx.doi.org/10.1016/j.jcv.2019.02.005DOI Listing
April 2019
4 Reads
3.016 Impact Factor

Performance evaluation of the Hologic Aptima HCV Quant Dx assay for detection of HCV RNA from dried blood spots.

J Clin Virol 2019 Mar 25;112:40-44. Epub 2019 Jan 25.

NSW State Reference Laboratory for HIV, St Vincent's Centre for Applied Medical Research, Darlinghurst, Sydney, Australia.

Background: The availability of effective direct-acting antiviral therapy for hepatitis C virus (HCV) has led to a need for simplified diagnostic pathways. Barriers to treatment uptake, specifically in people who inject drugs and in remote and resource limited settings, may be overcome by utilizing novel collection methods, such as dried blood spots (DBS). However, there are currently no registered assays for HCV RNA testing from DBS samples. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.010DOI Listing
March 2019
1 Read

A pan-genotypic Hepatitis C Virus NS5A amplification method for reliable genotyping and resistance testing.

J Clin Virol 2019 Apr 30;113:8-13. Epub 2019 Jan 30.

Institute of Virology, Heinrich-Heine-University, University Hospital, Düsseldorf, Germany.

Background: Chronic infection with the Hepatitis C Virus (HCV) is associated with the risk of progressive liver disease. Although, HCV treatment options and viral cure rates have tremendously increased over the last decade, all currently licensed combination therapies contain inhibitors of the replication complex NS5A. Resistance-associated substitutions (RAS) in NS5A can limit the efficacy of therapy; however, resistance testing is routinely not recommended for all patients. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.012DOI Listing
April 2019
9 Reads

The detection of BKPyV genotypes II and IV after renal transplantation as a simple tool for risk assessment for PyVAN and transplant outcome already at early stages of BKPyV reactivation.

J Clin Virol 2019 Apr 10;113:14-19. Epub 2019 Feb 10.

Institute for Virology, University Hospital Essen, University of Duisburg-Essen Virchowstr. 179, 45147, Essen, Germany.

Background: After reactivation the BK-polyomavirus (BKPyV) associated nephropathy (PyVAN) is observed in 1-10% of renal transplant recipients, of which up to 80% undergo graft failure. BKPyV reactivation after renal transplantation was associated with donor-derived serotypes against which the recipient has no immunological protection. However, PyVAN risk assessment seroactivity testing is a time-consuming and cost intensive process. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.02.002DOI Listing
April 2019
2 Reads

Intrahost viral evolution during chronic sapovirus infections.

J Clin Virol 2019 Apr 7;113:1-7. Epub 2019 Feb 7.

Institute of Virology, Leipzig University, Leipzig, Germany Johannisallee 30, 04103, Leipzig, Germany.

Background: Sapovirus is a common cause of self-limiting diarrhea. In immunocompromised individuals chronic infections occur, but are incompletely investigated.

Objectives: To investigate viral evolution in immunocompromised hosts during chronic sapovirus infection. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193002
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http://dx.doi.org/10.1016/j.jcv.2019.02.001DOI Listing
April 2019
8 Reads

Evaluation of a new rapid fluorescence immunoassay for the diagnosis of dengue and Zika virus infection.

J Clin Virol 2019 Mar 29;112:34-39. Epub 2019 Jan 29.

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Infectious and Tropical Diseases Unit, Careggi University and Hospital, Florence, Italy; Referral Center for Tropical Diseases of Tuscany, Infectious and Tropical Diseases Unit, Careggi University Hospital, Florence, Italy. Electronic address:

Background: Dengue (DENV) and Zika virus (ZIKV) are important mosquito-transmitted viruses.

Objectives: To investigate the performance of Standard F, Fluorescence Immunoassay (FIA, SD Biosensor Inc., Suwon, South Korea) providing results in 15 min to detect DENV IgG, IgM and NS1Ag, and ZIKV IgG, IgM, and Ag. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.011DOI Listing
March 2019
2 Reads

Determination of rubella virus-specific humoral and cell-mediated immunity in pregnant women with negative or equivocal rubella-specific IgG in routine screening.

J Clin Virol 2019 Mar 24;112:27-33. Epub 2019 Jan 24.

Groupe de Recherche sur les Infections pendant la Grossesse (GRIG), France; AP-HP, Hôpital Paul Brousse, Department of Virology, WHO Rubella NRL, 94804 Villejuif, France; Univ Paris-Sud, INSERM U1193, Villejuif, 94804 France. Electronic address:

Background: Immunity to rubella-virus (RV) is commonly determined by measuring specific IgG (RV-IgG). However, RV-IgG results may be different and even discordant, depending on the assay used. Cell-mediated immunity is not routinely investigated for diagnostic purposes. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.009DOI Listing
March 2019
1 Read

High epidemic burden of RSV disease coinciding with genetic alterations causing amino acid substitutions in the RSV G-protein during the 2016/2017 season in The Netherlands.

J Clin Virol 2019 Mar 23;112:20-26. Epub 2019 Jan 23.

University Medical Center Utrecht, Utrecht University, Department of Medical Microbiology, Utrecht 3584 CX, The Netherlands.

Background: We found amino acid substitutions in the Gglycoprotein of respiratory syncytial virus (RSV) A during the 2016/2017 epidemic in The Netherlands.

Objectives: We evaluated whether these alterations led to increased RSV incidence and disease burden.

Study Design: We sequenced the gene encoding the G-protein of prospectively collected clinical specimens from secondary care adult patients testing positive for RSV during the 2016/2017 and 2017/2018 epidemic RSV season. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.007DOI Listing
March 2019
6 Reads

Human metapneumovirus in paediatric intensive care unit (PICU) admissions in the United Kingdom (UK) 2006-2014.

J Clin Virol 2019 Mar 16;112:15-19. Epub 2019 Jan 16.

Oxford University Hospitals NHS Foundation Trust, Children's Hospital, Headley Way, Oxford, OX3 9DU, United Kingdom; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, OX3 9DU, United Kingdom; NIHR Oxford Biomedical Research Centre, Level 2, Children's Hospital, Oxford, OX3 9DU, United Kingdom.

Background: Human metapneumovirus (HMPV) is a pneumovirus known to cause respiratory disease in children. It was identified as a pathogen in 2001 and its healthcare burden and associated costs are not fully understood.

Objectives: This study aimed to assess the clinical characteristics of children with HMPV infection admitted to paediatric intensive care units (PICUs) across the United Kingdom (UK) over a nine-year period and to estimate the associated costs of care. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.006DOI Listing
March 2019
2 Reads

Performance characteristics of the VIDAS® ANTI-HEV IgM and IgG assays.

J Clin Virol 2019 Mar 11;112:10-14. Epub 2019 Jan 11.

CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, National Reference Center for Hepatitis E, F-31300, France; INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France.

Background: Several unautomated anti-HEV diagnostic tests are presently available.

Objective: We have evaluated the performance of the new automated VIDAS® ANTI-HEV IgM and IgG assays.

Study Design: We assessed the reproducibility and cross-reactivity of both VIDAS assays and the analytical sensitivity and linearity of the VIDAS IgG assay. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.005DOI Listing
March 2019
5 Reads

Challenges in management of epidemic keratoconjunctivitis with emerging recombinant human adenoviruses.

J Clin Virol 2019 Mar 11;112:1-9. Epub 2019 Jan 11.

Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan; Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan. Electronic address:

Adenoviral epidemic keratoconjunctivitis (EKC) presents as severe conjunctival inflammations involving the cornea that can lead to the development of corneal opacities and blurred vision, which can persist for months. EKC is highly contagious and responsible for outbreaks worldwide, therefore accurate diagnosis and rapid containment are imperative. EKC is caused by a number of types within Human adenovirus species D (HAdV-D): 8, 37 and 64 (formerly known as 19a) and these types were considered the major causes of EKC for over fifty years. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.004DOI Listing
March 2019
2 Reads

Analysis of serum hepatitis B virus RNA levels in a multiethnic cohort of pregnant chronic hepatitis B carriers.

J Clin Virol 2019 Feb 7;111:42-47. Epub 2019 Jan 7.

Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:

Background: Hepatitis B virus (HBV) flares have been reported due to alterations in the immune system during pregnancy. Recent studies in non-pregnant chronic hepatitis B (CHB) carriers have indicated that serum HBV RNA is a novel viral marker to assess treatment response and risk of disease flares.

Objectives: To analyze serum HBV RNA levels in association with established HBV markers in pregnant and/or post-partum CHB carriers. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193000
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http://dx.doi.org/10.1016/j.jcv.2019.01.002DOI Listing
February 2019
11 Reads
3.016 Impact Factor

Demonstration of the diagnostic agreement of capillary and venous blood samples, using hepatitis-C virus SD Bioline rapid test: A clinic-based study.

J Clin Virol 2019 Feb 31;111:39-41. Epub 2018 Dec 31.

Epicentre, Paris, France. Electronic address:

Background: Simplifying hepatitis C virus (HCV) screening is a key step in achieving the elimination of HCV as a global public health threat by 2030.

Objectives: The objective of this study was to demonstrate the agreement of capillary blood and venipuncture specimens when using SD Bioline HCV, a low-cost rapid diagnostic test (RDT), prequalified by WHO in 2016 on venous blood samples.

Study Design: Recruitment was conducted prospectively among adult patients presenting for HCV testing at the Médecins Sans Frontières (MSF) clinic of Preah Kossamak Hospital (Phnom Penh, Cambodia) between October and November 2017. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.008DOI Listing
February 2019
2 Reads

Performance of four diagnostic assays for detecting herpes simplex virus type 2 antibodies in the Middle East and North Africa.

J Clin Virol 2019 Feb 7;111:33-38. Epub 2019 Jan 7.

Infectious Disease Epidemiology Group, Weill Cornell Medicine-Qatar, Cornell University, Qatar Foundation - Education City, Doha, Qatar; Department of Healthcare Policy and Research, Weill Cornell Medicine, Cornell University, NY, USA; College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.

Background: Assessments of commercial assays in detecting herpes simplex virus type 2 (HSV-2) antibodies have shown variable sensitivity and specificity, and variation in performance by global population.

Objective: To evaluate performance of four assays in detecting HSV-2 antibodies in a composite Middle Eastern and North African (MENA) population. The assays are two ELISA kits: HerpeSelect 2 ELISA IgG and Euroimmun Anti-HSV-2 (gG2) ELISA (IgG), and two immunoblot (IB)/Western blot (WB) assays: HerpeSelect 1 and 2 Immunoblot IgG and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM). Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.001DOI Listing
February 2019
2 Reads
3.016 Impact Factor

Prospective evaluation of three rapid molecular tests for seasonal influenza in patients presenting at an emergency unit.

J Clin Virol 2019 Feb 7;111:29-32. Epub 2019 Jan 7.

Clinical Institute of Medical and Laboratory Diagnostics, Medical University of Graz, Austria.

Background: For infection control measures, rapid accurate diagnostics on admission of patients with suspected seasonal influenza is crucial.

Objective: Prospective comparison of three rapid molecular tests for detection of influenza A/B RNA.

Study Design: Outpatients presenting at the Medical emergency department of Graz University Hospital with influenza-like illness and a requirement for hospitalization (n = 312) were studied. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.003DOI Listing
February 2019
1 Read

A multicentre evaluation of the Elecsys HIV Duo assay.

J Clin Virol 2019 Mar 20;112:45-50. Epub 2018 Nov 20.

Labor Schottdorf MVZ GmbH, Abt RIA, Augsburg, Germany. Electronic address:

Background: Fourth generation HIV assays, which detect both HIV p24 antigen and HIV antibodies are widely used in HIV screening. The combination of markers enables the fourth generation assays to shorten the window of detection, which is important in real-world testing scenarios. The Elecsys HIV Duo assay is a fourth generation assay, which provides an overall result based on both the detection of the p24 antigen and HIV antibodies, and lists the sub-results for the antibody and antigen units. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.005DOI Listing
March 2019
1 Read

Performance evaluation of the Bio-Rad Geenius HIV 1/2 supplemental assay.

J Clin Virol 2019 Feb 19;111:24-28. Epub 2018 Dec 19.

Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA, United States. Electronic address:

Background: In the US, the HIV diagnostic algorithm for laboratory settings recommends the use of an HIV-1/HIV-2 differentiation supplemental assay after an initial reactive antigen/antibody (Ag/Ab) assay result. Since the discontinuation of the Multispot HIV-1/HIV-2 Rapid Test (MS), the Geenius HIV-1/2 Supplemental assay (Geenius) is the only FDA-approved supplemental differentiation test.

Objective: We compared the performance of Geenius to MS and Western Blot (WB). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183031
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http://dx.doi.org/10.1016/j.jcv.2018.12.006DOI Listing
February 2019
13 Reads
3.016 Impact Factor

Human adenovirus in nasopharyngeal and blood samples from children with and without respiratory tract infections.

J Clin Virol 2019 Feb 18;111:19-23. Epub 2018 Dec 18.

Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Norway; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Norway.

Background: Human adenovirus (HAdV) is a double-stranded DNA virus associated with respiratory tract infections (RTI) in children. Using polymerase chain reaction (PCR) tests, HAdV often is detected together with other virus species, even in healthy controls.

Objectives: The aim of this study was to compare molecular detection of HAdV with culture, and to examine the associations of various methods to RTI. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.005DOI Listing
February 2019
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Performance of Geno2Pheno[coreceptor] to infer coreceptor use in human immunodeficiency virus type 1 (HIV-1) subtype A.

J Clin Virol 2019 Feb 19;111:12-18. Epub 2018 Dec 19.

Department of Medical Biotechnologies, University of Siena, Siena, Italy.

Background: Assessment of human immunodeficiency virus type 1 (HIV-1) coreceptor usage is required prior to treatment with the CCR5 antagonist maraviroc to exclude the presence of CXCR4-using (X4) strains. Genotype-based interpretation systems are mostly designed on subtype B and have been reported to be less accurate for subtype A/CRF02_AG.

Objectives: To evaluate the performance of the widely used Geno2Pheno[coreceptor] (G2P[c]) algorithm for prediction of coreceptor usage with subtype A/CRF02_AG vs. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.007DOI Listing
February 2019
1 Read

Viral gastroenteritis among children of 0-5 years in Nigeria: Characterization of the first Nigerian aichivirus, recombinant noroviruses and detection of a zoonotic astrovirus.

J Clin Virol 2019 Feb 17;111:4-11. Epub 2018 Dec 17.

Department of Infectious Diseases, Unit of Viral Gastroenteritis and Hepatitis Pathogens and Enteroviruses, Robert Koch Institute, Berlin, Germany. Electronic address:

Background: Viruses are the leading cause of acute gastroenteritis in children worldwide. Understanding of the occurrence and genetic diversity of these viruses can help to prevent infections.

Objectives: The present study describes the presence, genetic diversity and possible recombination of five enteric viruses in children with gastroenteritis in Southwestern Nigeria. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.004DOI Listing
February 2019
6 Reads

Performance evaluation of the re-standardized Abbott Architect anti-HBs assay.

J Clin Virol 2019 Feb 15;111:1-3. Epub 2018 Dec 15.

Department of Microbiology, Prince of Wales Hospital, Hong Kong, China.

Background: As defined by World Health Organization (WHO), an antibody level of ≥ 10mIU/mL to hepatitis B virus confers protection. With the launching of Abbott anti-HBs assay re-standardized to the 2 WHO International Reference Preparation, a positive bias in antibody level would be anticipated. Manufacturer provides limited data for samples around the immune cut-off which has potential implication on vaccine guidance. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183031
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http://dx.doi.org/10.1016/j.jcv.2018.12.003DOI Listing
February 2019
2 Reads

Evolution of symptoms and quality of life during Zika virus infection: A 1-year prospective cohort study.

J Clin Virol 2018 Dec 26;109:57-62. Epub 2018 Sep 26.

French Armed Forces Health Service in French Guiana, Cayenne, French Guiana; Unité de Parasitologie et Entomologie, Département des Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, Marseille, France; Aix Marseille Université, IRD, AP-HM, SSA, UMR Vecteurs - Infections Tropicales et Méditerranéennes (VITROME), IHU - Méditerranée Infection, Marseille, France.

Background: Although the complications of Zika virus infection have been well described, the clinical pattern has not been reported in enough detail to differentiate this infection from those with other arboviroses, and no longitudinal study has yet been published on the persistence of symptoms and quality of life.

Objectives: were to describe bio-clinical pattern and quality of life during ZIKV infection, and their evolution.

Study Design: We present a 1-year clinical follow-up of 49 people infected with Zika virus in French Guiana, for whom the diagnosis was confirmed by RT-PCR in serum or urine. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.015DOI Listing
December 2018
4 Reads

Is antibody-mediated rejection in kidney transplant recipients a risk factor for developing cytomegalovirus or BK virus infection? Results from a case-control study.

J Clin Virol 2019 Jan 1;110:45-50. Epub 2018 Dec 1.

Infectious Diseases Department, Vall d'Hebron University Hospital, Barcelona, Spain; Departament of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain.

Background: Data are scarce on cytomegalovirus (CMV) and BK virus (BKV) infection after antibody-mediated rejection (ABMR).

Objectives: We hypothesized that the immunological response in patients with ABMR or the immune modulation associated with its treatment could predispose to CMV and BKV infection. Our objective was to investigate this hypothesis. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.010DOI Listing
January 2019
8 Reads

Problematic molecular diagnosis of HSV-1 infection due to a single nucleotide polymorphism in the US7 gene.

J Clin Virol 2019 Jan 3;110:42-44. Epub 2018 Dec 3.

Virology Laboratory, Centre Hospitalier Universitaire de Nice, 151 Route Saint Antoine Ginestiere, 06202 Nice Cedex 3, France. Electronic address:

Background: HSV-1 infection is very common worldwide and can be associated with severe medical conditions such as encephalitis and neonatal herpes. Current diagnosis relies heavily on molecular assays targeting the viral genome. One of the pitfalls of these assays is genetic variability, as viral polymorphisms located in the target region can impair the detection of HSV-1. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.001DOI Listing
January 2019
3 Reads

Comparative performance of the Biocentric Generic Viral Load, Roche CAP/CTM v1.5, Roche CAP/CTM v2.0 and m2000 Abbott assays for quantifying HIV-1 B and non-B strains: Underestimation of some CRF02 strains.

J Clin Virol 2019 Jan 3;110:36-41. Epub 2018 Dec 3.

Université Paris Descartes, EA 7327, Sorbonne Paris Cité, AP-HP, Laboratoire de Virologie, Hôpital Necker, Paris, France. Electronic address:

Background: HIV-1 viral load testing is now recommended by the World Health Organization for every patient receiving antiretroviral therapy (ART).

Objectives: The objective of this study is to evaluate the performance of commercial assays for their ability to quantify HIV-1 strains currently circulating in France.

Study Design: The performances of the Generic HIV-RNA assay from Biocentric were compared to those of the Roche CAP/CTM v1. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.002DOI Listing
January 2019
1 Read