4,108 results match your criteria Journal of Clinical Virology[Journal]


A pan-genotypic Hepatitis C Virus NS5A amplification method for reliable genotyping and resistance testing.

J Clin Virol 2019 Jan 30;113:8-13. Epub 2019 Jan 30.

Institute of Virology, Heinrich-Heine-University, University Hospital, Düsseldorf, Germany.

Background: Chronic infection with the Hepatitis C Virus (HCV) is associated with the risk of progressive liver disease. Although, HCV treatment options and viral cure rates have tremendously increased over the last decade, all currently licensed combination therapies contain inhibitors of the replication complex NS5A. Resistance-associated substitutions (RAS) in NS5A can limit the efficacy of therapy; however, resistance testing is routinely not recommended for all patients. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193002
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http://dx.doi.org/10.1016/j.jcv.2019.01.012DOI Listing
January 2019
1 Read

The detection of BKPyV genotypes II and IV after renal transplantation as a simple tool for risk assessment for PyVAN and transplant outcome already at early stages of BKPyV reactivation.

J Clin Virol 2019 Feb 10;113:14-19. Epub 2019 Feb 10.

Institute for Virology, University Hospital Essen, University of Duisburg-Essen Virchowstr. 179, 45147, Essen, Germany.

Background: After reactivation the BK-polyomavirus (BKPyV) associated nephropathy (PyVAN) is observed in 1-10% of renal transplant recipients, of which up to 80% undergo graft failure. BKPyV reactivation after renal transplantation was associated with donor-derived serotypes against which the recipient has no immunological protection. However, PyVAN risk assessment seroactivity testing is a time-consuming and cost intensive process. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.02.002DOI Listing
February 2019

Intrahost viral evolution during chronic sapovirus infections.

J Clin Virol 2019 Feb 7;113:1-7. Epub 2019 Feb 7.

Institute of Virology, Leipzig University, Leipzig, Germany Johannisallee 30, 04103, Leipzig, Germany.

Background: Sapovirus is a common cause of self-limiting diarrhea. In immunocompromised individuals chronic infections occur, but are incompletely investigated.

Objectives: To investigate viral evolution in immunocompromised hosts during chronic sapovirus infection. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.02.001DOI Listing
February 2019
1 Read

Evaluation of a new rapid fluorescence immunoassay for the diagnosis of dengue and Zika virus infection.

J Clin Virol 2019 Jan 29;112:34-39. Epub 2019 Jan 29.

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Infectious and Tropical Diseases Unit, Careggi University and Hospital, Florence, Italy; Referral Center for Tropical Diseases of Tuscany, Infectious and Tropical Diseases Unit, Careggi University Hospital, Florence, Italy. Electronic address:

Background: Dengue (DENV) and Zika virus (ZIKV) are important mosquito-transmitted viruses.

Objectives: To investigate the performance of Standard F, Fluorescence Immunoassay (FIA, SD Biosensor Inc., Suwon, South Korea) providing results in 15 min to detect DENV IgG, IgM and NS1Ag, and ZIKV IgG, IgM, and Ag. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.011DOI Listing
January 2019
1 Read

Determination of rubella virus-specific humoral and cell-mediated immunity in pregnant women with negative or equivocal rubella-specific IgG in routine screening.

J Clin Virol 2019 Jan 24;112:27-33. Epub 2019 Jan 24.

Groupe de Recherche sur les Infections pendant la Grossesse (GRIG), France; AP-HP, Hôpital Paul Brousse, Department of Virology, WHO Rubella NRL, 94804 Villejuif, France; Univ Paris-Sud, INSERM U1193, Villejuif, 94804 France. Electronic address:

Background: Immunity to rubella-virus (RV) is commonly determined by measuring specific IgG (RV-IgG). However, RV-IgG results may be different and even discordant, depending on the assay used. Cell-mediated immunity is not routinely investigated for diagnostic purposes. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.009DOI Listing
January 2019

High epidemic burden of RSV disease coinciding with genetic alterations causing amino acid substitutions in the RSV G-protein during the 2016/2017 season in The Netherlands.

J Clin Virol 2019 Jan 23;112:20-26. Epub 2019 Jan 23.

University Medical Center Utrecht, Utrecht University, Department of Medical Microbiology, Utrecht 3584 CX, The Netherlands.

Background: We found amino acid substitutions in the Gglycoprotein of respiratory syncytial virus (RSV) A during the 2016/2017 epidemic in The Netherlands.

Objectives: We evaluated whether these alterations led to increased RSV incidence and disease burden.

Study Design: We sequenced the gene encoding the G-protein of prospectively collected clinical specimens from secondary care adult patients testing positive for RSV during the 2016/2017 and 2017/2018 epidemic RSV season. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.007DOI Listing
January 2019

Human metapneumovirus in paediatric intensive care unit (PICU) admissions in the United Kingdom (UK) 2006-2014.

J Clin Virol 2019 Jan 16;112:15-19. Epub 2019 Jan 16.

Oxford University Hospitals NHS Foundation Trust, Children's Hospital, Headley Way, Oxford, OX3 9DU, United Kingdom; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, OX3 9DU, United Kingdom; NIHR Oxford Biomedical Research Centre, Level 2, Children's Hospital, Oxford, OX3 9DU, United Kingdom.

Background: Human metapneumovirus (HMPV) is a pneumovirus known to cause respiratory disease in children. It was identified as a pathogen in 2001 and its healthcare burden and associated costs are not fully understood.

Objectives: This study aimed to assess the clinical characteristics of children with HMPV infection admitted to paediatric intensive care units (PICUs) across the United Kingdom (UK) over a nine-year period and to estimate the associated costs of care. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.006DOI Listing
January 2019
1 Read

Performance characteristics of the VIDAS® ANTI-HEV IgM and IgG assays.

J Clin Virol 2019 Jan 11;112:10-14. Epub 2019 Jan 11.

CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, National Reference Center for Hepatitis E, F-31300, France; INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France.

Background: Several unautomated anti-HEV diagnostic tests are presently available.

Objective: We have evaluated the performance of the new automated VIDAS® ANTI-HEV IgM and IgG assays.

Study Design: We assessed the reproducibility and cross-reactivity of both VIDAS assays and the analytical sensitivity and linearity of the VIDAS IgG assay. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.005DOI Listing
January 2019
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Challenges in management of epidemic keratoconjunctivitis with emerging recombinant human adenoviruses.

J Clin Virol 2019 Jan 11;112:1-9. Epub 2019 Jan 11.

Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan; Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan. Electronic address:

Adenoviral epidemic keratoconjunctivitis (EKC) presents as severe conjunctival inflammations involving the cornea that can lead to the development of corneal opacities and blurred vision, which can persist for months. EKC is highly contagious and responsible for outbreaks worldwide, therefore accurate diagnosis and rapid containment are imperative. EKC is caused by a number of types within Human adenovirus species D (HAdV-D): 8, 37 and 64 (formerly known as 19a) and these types were considered the major causes of EKC for over fifty years. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.004DOI Listing
January 2019

Analysis of serum hepatitis B virus RNA levels in a multiethnic cohort of pregnant chronic hepatitis B carriers.

J Clin Virol 2019 Feb 7;111:42-47. Epub 2019 Jan 7.

Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:

Background: Hepatitis B virus (HBV) flares have been reported due to alterations in the immune system during pregnancy. Recent studies in non-pregnant chronic hepatitis B (CHB) carriers have indicated that serum HBV RNA is a novel viral marker to assess treatment response and risk of disease flares.

Objectives: To analyze serum HBV RNA levels in association with established HBV markers in pregnant and/or post-partum CHB carriers. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193000
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http://dx.doi.org/10.1016/j.jcv.2019.01.002DOI Listing
February 2019
4 Reads

Demonstration of the diagnostic agreement of capillary and venous blood samples, using hepatitis-C virus SD Bioline rapid test: A clinic-based study.

J Clin Virol 2019 Feb 31;111:39-41. Epub 2018 Dec 31.

Epicentre, Paris, France. Electronic address:

Background: Simplifying hepatitis C virus (HCV) screening is a key step in achieving the elimination of HCV as a global public health threat by 2030.

Objectives: The objective of this study was to demonstrate the agreement of capillary blood and venipuncture specimens when using SD Bioline HCV, a low-cost rapid diagnostic test (RDT), prequalified by WHO in 2016 on venous blood samples.

Study Design: Recruitment was conducted prospectively among adult patients presenting for HCV testing at the Médecins Sans Frontières (MSF) clinic of Preah Kossamak Hospital (Phnom Penh, Cambodia) between October and November 2017. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.008DOI Listing
February 2019

Performance of four diagnostic assays for detecting herpes simplex virus type 2 antibodies in the Middle East and North Africa.

J Clin Virol 2019 Feb 7;111:33-38. Epub 2019 Jan 7.

Infectious Disease Epidemiology Group, Weill Cornell Medicine-Qatar, Cornell University, Qatar Foundation - Education City, Doha, Qatar; Department of Healthcare Policy and Research, Weill Cornell Medicine, Cornell University, NY, USA; College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.

Background: Assessments of commercial assays in detecting herpes simplex virus type 2 (HSV-2) antibodies have shown variable sensitivity and specificity, and variation in performance by global population.

Objective: To evaluate performance of four assays in detecting HSV-2 antibodies in a composite Middle Eastern and North African (MENA) population. The assays are two ELISA kits: HerpeSelect 2 ELISA IgG and Euroimmun Anti-HSV-2 (gG2) ELISA (IgG), and two immunoblot (IB)/Western blot (WB) assays: HerpeSelect 1 and 2 Immunoblot IgG and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM). Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.001DOI Listing
February 2019
3.016 Impact Factor

Prospective evaluation of three rapid molecular tests for seasonal influenza in patients presenting at an emergency unit.

J Clin Virol 2019 Feb 7;111:29-32. Epub 2019 Jan 7.

Clinical Institute of Medical and Laboratory Diagnostics, Medical University of Graz, Austria.

Background: For infection control measures, rapid accurate diagnostics on admission of patients with suspected seasonal influenza is crucial.

Objective: Prospective comparison of three rapid molecular tests for detection of influenza A/B RNA.

Study Design: Outpatients presenting at the Medical emergency department of Graz University Hospital with influenza-like illness and a requirement for hospitalization (n = 312) were studied. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.01.003DOI Listing
February 2019

A multicentre evaluation of the Elecsys HIV Duo assay.

J Clin Virol 2018 Nov 20. Epub 2018 Nov 20.

Labor Schottdorf MVZ GmbH, Abt RIA, Augsburg, Germany. Electronic address:

Background: Fourth generation HIV assays, which detect both HIV p24 antigen and HIV antibodies are widely used in HIV screening. The combination of markers enables the fourth generation assays to shorten the window of detection, which is important in real-world testing scenarios. The Elecsys HIV Duo assay is a fourth generation assay, which provides an overall result based on both the detection of the p24 antigen and HIV antibodies, and lists the sub-results for the antibody and antigen units. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.005DOI Listing
November 2018

Performance evaluation of the Bio-Rad Geenius HIV 1/2 supplemental assay.

J Clin Virol 2019 Feb 19;111:24-28. Epub 2018 Dec 19.

Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA, United States. Electronic address:

Background: In the US, the HIV diagnostic algorithm for laboratory settings recommends the use of an HIV-1/HIV-2 differentiation supplemental assay after an initial reactive antigen/antibody (Ag/Ab) assay result. Since the discontinuation of the Multispot HIV-1/HIV-2 Rapid Test (MS), the Geenius HIV-1/2 Supplemental assay (Geenius) is the only FDA-approved supplemental differentiation test.

Objective: We compared the performance of Geenius to MS and Western Blot (WB). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183031
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http://dx.doi.org/10.1016/j.jcv.2018.12.006DOI Listing
February 2019
7 Reads
3.016 Impact Factor

Human adenovirus in nasopharyngeal and blood samples from children with and without respiratory tract infections.

J Clin Virol 2019 Feb 18;111:19-23. Epub 2018 Dec 18.

Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Norway; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Norway.

Background: Human adenovirus (HAdV) is a double-stranded DNA virus associated with respiratory tract infections (RTI) in children. Using polymerase chain reaction (PCR) tests, HAdV often is detected together with other virus species, even in healthy controls.

Objectives: The aim of this study was to compare molecular detection of HAdV with culture, and to examine the associations of various methods to RTI. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.005DOI Listing
February 2019

Performance of Geno2Pheno[coreceptor] to infer coreceptor use in human immunodeficiency virus type 1 (HIV-1) subtype A.

J Clin Virol 2019 Feb 19;111:12-18. Epub 2018 Dec 19.

Department of Medical Biotechnologies, University of Siena, Siena, Italy.

Background: Assessment of human immunodeficiency virus type 1 (HIV-1) coreceptor usage is required prior to treatment with the CCR5 antagonist maraviroc to exclude the presence of CXCR4-using (X4) strains. Genotype-based interpretation systems are mostly designed on subtype B and have been reported to be less accurate for subtype A/CRF02_AG.

Objectives: To evaluate the performance of the widely used Geno2Pheno[coreceptor] (G2P[c]) algorithm for prediction of coreceptor usage with subtype A/CRF02_AG vs. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.007DOI Listing
February 2019

Viral gastroenteritis among children of 0-5 years in Nigeria: Characterization of the first Nigerian aichivirus, recombinant noroviruses and detection of a zoonotic astrovirus.

J Clin Virol 2019 Feb 17;111:4-11. Epub 2018 Dec 17.

Department of Infectious Diseases, Unit of Viral Gastroenteritis and Hepatitis Pathogens and Enteroviruses, Robert Koch Institute, Berlin, Germany. Electronic address:

Background: Viruses are the leading cause of acute gastroenteritis in children worldwide. Understanding of the occurrence and genetic diversity of these viruses can help to prevent infections.

Objectives: The present study describes the presence, genetic diversity and possible recombination of five enteric viruses in children with gastroenteritis in Southwestern Nigeria. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.004DOI Listing
February 2019
5 Reads

Performance evaluation of the re-standardized Abbott Architect anti-HBs assay.

J Clin Virol 2019 Feb 15;111:1-3. Epub 2018 Dec 15.

Department of Microbiology, Prince of Wales Hospital, Hong Kong, China.

Background: As defined by World Health Organization (WHO), an antibody level of ≥ 10mIU/mL to hepatitis B virus confers protection. With the launching of Abbott anti-HBs assay re-standardized to the 2 WHO International Reference Preparation, a positive bias in antibody level would be anticipated. Manufacturer provides limited data for samples around the immune cut-off which has potential implication on vaccine guidance. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183031
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http://dx.doi.org/10.1016/j.jcv.2018.12.003DOI Listing
February 2019
1 Read

Evolution of symptoms and quality of life during Zika virus infection: A 1-year prospective cohort study.

J Clin Virol 2018 Dec 26;109:57-62. Epub 2018 Sep 26.

French Armed Forces Health Service in French Guiana, Cayenne, French Guiana; Unité de Parasitologie et Entomologie, Département des Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, Marseille, France; Aix Marseille Université, IRD, AP-HM, SSA, UMR Vecteurs - Infections Tropicales et Méditerranéennes (VITROME), IHU - Méditerranée Infection, Marseille, France.

Background: Although the complications of Zika virus infection have been well described, the clinical pattern has not been reported in enough detail to differentiate this infection from those with other arboviroses, and no longitudinal study has yet been published on the persistence of symptoms and quality of life.

Objectives: were to describe bio-clinical pattern and quality of life during ZIKV infection, and their evolution.

Study Design: We present a 1-year clinical follow-up of 49 people infected with Zika virus in French Guiana, for whom the diagnosis was confirmed by RT-PCR in serum or urine. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.015DOI Listing
December 2018
1 Read

Is antibody-mediated rejection in kidney transplant recipients a risk factor for developing cytomegalovirus or BK virus infection? Results from a case-control study.

J Clin Virol 2019 Jan 1;110:45-50. Epub 2018 Dec 1.

Infectious Diseases Department, Vall d'Hebron University Hospital, Barcelona, Spain; Departament of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain.

Background: Data are scarce on cytomegalovirus (CMV) and BK virus (BKV) infection after antibody-mediated rejection (ABMR).

Objectives: We hypothesized that the immunological response in patients with ABMR or the immune modulation associated with its treatment could predispose to CMV and BKV infection. Our objective was to investigate this hypothesis. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.010DOI Listing
January 2019
7 Reads

Problematic molecular diagnosis of HSV-1 infection due to a single nucleotide polymorphism in the US7 gene.

J Clin Virol 2019 Jan 3;110:42-44. Epub 2018 Dec 3.

Virology Laboratory, Centre Hospitalier Universitaire de Nice, 151 Route Saint Antoine Ginestiere, 06202 Nice Cedex 3, France. Electronic address:

Background: HSV-1 infection is very common worldwide and can be associated with severe medical conditions such as encephalitis and neonatal herpes. Current diagnosis relies heavily on molecular assays targeting the viral genome. One of the pitfalls of these assays is genetic variability, as viral polymorphisms located in the target region can impair the detection of HSV-1. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.001DOI Listing
January 2019
1 Read

Comparative performance of the Biocentric Generic Viral Load, Roche CAP/CTM v1.5, Roche CAP/CTM v2.0 and m2000 Abbott assays for quantifying HIV-1 B and non-B strains: Underestimation of some CRF02 strains.

J Clin Virol 2019 Jan 3;110:36-41. Epub 2018 Dec 3.

Université Paris Descartes, EA 7327, Sorbonne Paris Cité, AP-HP, Laboratoire de Virologie, Hôpital Necker, Paris, France. Electronic address:

Background: HIV-1 viral load testing is now recommended by the World Health Organization for every patient receiving antiretroviral therapy (ART).

Objectives: The objective of this study is to evaluate the performance of commercial assays for their ability to quantify HIV-1 strains currently circulating in France.

Study Design: The performances of the Generic HIV-RNA assay from Biocentric were compared to those of the Roche CAP/CTM v1. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.12.002DOI Listing
January 2019

Surveillance of enteroviruses from paediatric patients attended at a tertiary hospital in Catalonia from 2014 to 2017.

J Clin Virol 2019 Jan 30;110:29-35. Epub 2018 Nov 30.

Respiratory Viruses Unit, Virology Section, Microbiology Department, Hospital Universitari Vall d'Hebron, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona, Barcelona, Spain.

Background: Enterovirus (EV) infections are usually asymptomatic or mild, but symptomatic infections can evolve to severe complications. Outbreaks of EV-A71 and EV-D68 have been recently reported worldwide, sometimes related to severe clinical outcomes.

Objective: To describe EV genetic diversity and the clinical outcomes from paediatric patients attended at a tertiary university hospital in Barcelona (Catalonia, Spain) from 2014 to 2017. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.004DOI Listing
January 2019
3 Reads

Development and evaluation of a BK polyomavirus serotyping assay using Luminex technology.

J Clin Virol 2019 Jan 1;110:22-28. Epub 2018 Dec 1.

Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands.

Background: The BK polyomavirus (BKPyV) is subdivided into four genotypes. The consequences of each genotype and of donor-recipient genotype (mis)match for BKPyV-associated nephropathy (BKPyVAN) in kidney transplant recipients (KTRs) are unknown.

Objectives: To develop and evaluate a genotype-specific IgG antibody-based BKPyV serotyping assay, in order to classify kidney transplant donors and recipients accordingly. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.009DOI Listing
January 2019

Editorial.

Authors:
Bert Niesters

J Clin Virol 2019 Jan 27;110:A1. Epub 2018 Nov 27.

University Medical Center Groningen, Department of Medical Microbiology, Division of Clinical Virology, the Netherlands. Electronic address:

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http://dx.doi.org/10.1016/j.jcv.2018.11.007DOI Listing
January 2019

Impact of multiplex molecular assay turn-around-time on antibiotic utilization and clinical management of hospitalized children with acute respiratory tract infections.

J Clin Virol 2019 Jan 23;110:11-16. Epub 2018 Nov 23.

Department of Pathology and Laboratory Medicine, Children's Hospitals, Kansas City, MO, United States; School of Medicine, University of Missouri, Kansas City, MO, United States.

Background: Empiric antibiotic treatment is common among children with acute respiratory tract infections (ARTI), despite infections being predominately viral. The use of molecular respiratory panel assays has become increasingly common for medical care of patients with ARTIs.

Study Design: This was a 6-year retrospective, single-centered study of pediatric inpatients who tested positive for an ARTI respiratory pathogen. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.006DOI Listing
January 2019

Varicella-zoster virus clades circulating in Spain over two decades.

J Clin Virol 2019 Jan 28;110:17-21. Epub 2018 Nov 28.

Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III, Majadahonda, Spain. Electronic address:

Background: Despite childhood universal VZV immunization was introduced in 2015, there are no data on VZV clade distribution in Spain.

Objectives: To characterize the varicella-zoster virus strains circulating in Spain between 1997 and 2016.

Study Design: In this retrospective study, we determined the VZV clades in 294 patients with different pathologies (mainly encephalitis, zoster and varicella) by sequencing three fragments within ORF 22, ORF 21 and ORF 50 and, subsequently analyzing 7 relevant SNPs. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.008DOI Listing
January 2019
5 Reads
3.016 Impact Factor

Detection of glycoproteins B and H genotypes to predict the development of Cytomegalovirus disease in solid organ transplant recipients.

J Clin Virol 2018 Dec 13;109:50-56. Epub 2018 Nov 13.

Virology Laboratory, Clinical Microbiology Department, University Hospital 12 de Octubre, Avda. de Córdoba s/n, 28041 Madrid, Spain; Biomedical Research Institute i+12, University Hospital 12 de Octubre, Avda. de Córdoba, s/n, 28041 Madrid, Spain; Department of Medicine, School of Medicine, Complutense University, Pl. Ramón y Cajal, s/n, 28040 Madrid, Spain.

Background: Our study focuses on the role that human Cytomegalovirus (CMV) genotypes play in the development of disease.

Objectives: (1) To analyze the frequency of various genotype envelope proteins (gB, gH) in a group of solid organ transplant (SOT) recipients; (2) to assess their correlation with CMV disease; (3) to study the association between any of the genotypes and viral loads.

Study Design: A retrospective observational study conducted by analyzing CMV gB and gH genotypes detected with real-time polymerase chain reaction (PCR)-specific assays in 162 CMV-positive blood samples from 62 SOT recipients. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.001DOI Listing
December 2018

Evaluation of the Abbott m2000 system for dried blood spot detection of hepatitis C virus RNA.

J Clin Virol 2019 Jan 3;110:7-10. Epub 2018 Nov 3.

West of Scotland Specialist Virology Centre, Level 5 New Lister Building, Glasgow Royal Infirmary, Alexandra Parade, Glasgow, G31 2ER, United Kingdom.

Background: Hepatitis C virus RNA testing using dried blood spots (DBS) offers a method for detecting ongoing HCV infection in "hard to reach" populations. Abbott Molecular have developed a quantitative HCV RNA DBS protocol (currently for research use only) for extraction and real-time PCR amplification using them2000sp and m2000rt system.

Methods: A panel of seventy "mock" DBS were made from patient whole blood; who were known to be either HCV RNA negative or positive. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183027
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http://dx.doi.org/10.1016/j.jcv.2018.10.008DOI Listing
January 2019
8 Reads

Severe atypical hand-foot-and-mouth disease in adults due to coxsackievirus A6: Clinical presentation and phylogenesis of CV-A6 strains.

J Clin Virol 2019 Jan 17;110:1-6. Epub 2018 Nov 17.

Clinical Microbiology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.

Background: Typically, hand-foot-and-mouth disease (HFMD) is a mild childhood illness associated with coxsackievirus (CV)-A16, CV-A6, enterovirus (EV)-A71.

Objectives: To identify the viral agents associated with severe cases of atypical HFMD in Italy.

Study Design: Epidemiologically unrelated cases of severe atypical HFMD admitted to the Emergency Room (ER) of IRCCS San Martino IST (Genoa, Italy) in 2014-2016 were investigated. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.003DOI Listing
January 2019
12 Reads

HIV-RNA decay in paired blood and semen samples of subjects receiving their first dolutegravir-based ART regimen.

J Clin Virol 2018 Dec 15;109:45-49. Epub 2018 Nov 15.

University of Bari 'Aldo Moro', Clinic of Infectious Diseases, Bari, Italy.

Background And Objectives: We aimed to investigate to what extent a first-line DTG-based ART regimen reduces HIV-RNA in semen compared to plasma.

Study Design: In this prospective, observational study, ART-naïve, HIV-infected males starting their first ART regimen with DTG plus TDF/FTC or ABC/3TC were enrolled. Paired blood (BP) and seminal plasma (SP) samples were collected at baseline (T0) and at week-2/4/12/24 after ART initiation. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.11.002DOI Listing
December 2018
6 Reads

A single-center, single-blind study to evaluate the clinical sensitivity, specificity, and agreement between Elecsys Anti-HBc II and Elecsys Anti-HBc in a Korean population.

J Clin Virol 2018 Dec 28;109:41-44. Epub 2018 Oct 28.

Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. Electronic address:

Background: Anti-HBc IgG is almost always detected in hepatitis B virus (HBV)-infected individuals and persists in resolved infections. In certain cases, anti-HBc IgG is the only serological marker and anti-HBc-positive result generally means anti-HBc total positivity.

Objectives: To evaluate the clinical sensitivity and specificity of an investigational medical device, Elecsys Anti-HBc II, using samples from the Korean population. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.009DOI Listing
December 2018

Surveillance and molecular characterization of human adenovirus in patients with acute gastroenteritis in the era of rotavirus vaccine, Brazil, 2012-2017.

J Clin Virol 2018 Dec 1;109:35-40. Epub 2018 Nov 1.

Adolfo Lutz Institute, Virology Centre, Enteric Diseases Laboratory, São Paulo, SP, Brazil. Electronic address:

Background: In Brazil, limited data are available regarding the incidence and molecular epidemiology of Human Adenovirus (HAdV) in diarrheic disease, especially in the post rotavirus (RVA) vaccine era.

Objective: The aims of the study were to investigate the frequency of HAdV infections in patients with gastroenteritis during a 6-year period (2012-2017); conduct molecular typing of positive strains, and obtain further information on the HAdV seasonality.

Study Design: A total of 3003 fecal samples negative for both, RVA and Norovirus, were selected and tested for HAdV by PCR. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.010DOI Listing
December 2018
3 Reads

Usefulness of a serial algorithm of HBsAg and HBeAg rapid diagnosis tests to detect pregnant women at risk of HBV mother-to-child transmission in Cambodia, the ANRS 12328 pilot study.

J Clin Virol 2018 12 22;109:29-34. Epub 2018 Oct 22.

Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.

Background: In Cambodia, access to hepatitis B surface antigen (HBsAg) screening is low for pregnant women and Hepatitis B Virus (HBV) DNA quantification is poorly accessible.

Objectives: To evaluate the performance of a serial algorithm using two HBV rapid diagnostic tests (RDTs), in which samples positive for HBsAg were further tested for HBeAg as a surrogate marker for HBV DNA quantification.

Study Design: In 2015, we prospectively collected plasma samples from 250 pregnant women consulting for antenatal care in one hospital in Phnom Penh including 128 with a known positive HBsAg status. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183026
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http://dx.doi.org/10.1016/j.jcv.2018.10.007DOI Listing
December 2018
6 Reads

Evaluation of performance characteristics of hepatitis B e antigen serologic assays.

J Clin Virol 2018 Dec 13;109:22-28. Epub 2018 Oct 13.

Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA 30329 United States. Electronic address:

Background: Hepatitis B e antigen (HBeAg) is considered an indicator of high hepatitis B virus (HBV) replication. Performance characteristics of commercially available HBeAg assays have not been determined, thus it is unknown whether lack of HBeAg detection is because of test sensitivity or HBV basal core promoter and precore mutations.

Objectives: We studied the correlation between HBeAg reactivity with HBV DNA levels in three commercially available HBeAg assays using 335 HBsAg and HBV DNA positive serum/plasma samples. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.005DOI Listing
December 2018
1 Read

Diagnosis of rotavirus infection in a vaccinated population: Is a less sensitive immunochromatographic method more suitable for detecting wild-type rotavirus than real-time RT-PCR?

J Clin Virol 2018 Dec 18;109:19-21. Epub 2018 Oct 18.

UCD National Virus Reference Laboratory, University College Dublin, Dublin 4, Ireland.

Background: Diagnosis of wild-type rotavirus disease may be complicated by the detection of vaccine-derived virus which can be detected in stool samples following immunisation. We evaluate an immunochromatographic assay and real-time RT-PCR to determine which is more suitable for the detection of wild-type rotavirus.

Objectives: To compare the Ct values of wild-type rotavirus and Rotarix determined by real-time RT-PCR. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.006DOI Listing
December 2018
12 Reads

HPV genotyping and E6/E7 transcript assays for cervical lesion detection in an Asian screening population-Cobas and Aptima HPV tests.

J Clin Virol 2018 Dec 13;109:13-18. Epub 2018 Oct 13.

Department of Pathology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. Electronic address:

Background: High-risk human papillomavirus (hrHPV) detection and genotyping by Cobas HPV test has become an important technical platform in cervical cancer screening. It may be used as a co-test with cervical cytology or as a standalone test. Aptima HPV assay (AHPV) is another hrHPV test detecting 13 genotypes through qPCR based amplification of viral E6/E7 transcripts. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183025
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http://dx.doi.org/10.1016/j.jcv.2018.10.004DOI Listing
December 2018
37 Reads

Risk factors for BK virus viremia and nephropathy after kidney transplantation: A systematic review.

J Clin Virol 2018 Dec 12;109:6-12. Epub 2018 Oct 12.

Department of Virology, Amiens University Medical Center, Amiens, France; AGIR Research Unit, EA 4294, Jules Verne University of Picardie, Amiens, France. Electronic address:

In the last 20 years, the management of BK polyomavirus (BKPyV) reactivation in kidney transplant patients has become a true challenge for the transplant community. The only treatment option is based on the early identification of at-risk patients. The number of reported risk factors for BKPyV reactivation has increased markedly in the literature last years, although they are sometimes in an unclear or contradictory manner. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.002DOI Listing
December 2018
18 Reads

Performance of a commercial assay for detecting and quantifying HEV RNA in faeces.

J Clin Virol 2018 Dec 10;109:1-5. Epub 2018 Oct 10.

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, National Reference Center for Hepatitis E, F-31300, France.

Background: Detecting hepatitis E virus (HEV) RNA in faeces is useful for diagnosing and monitoring HEV infections, particularly in immunocompromised patients requiring ribavirin therapy.

Objectives: This study evaluated the performance of the Altona RealStar HEV RNA kit for detecting and quantifying HEV in faeces.

Study Design: RNA was extracted from 94 stool samples by two methods: QIAamp Viral RNA Mini kit and MagNA Pure 96 automate. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.003DOI Listing
December 2018
11 Reads

Neonatal HCMV-related polymicrogyria in seroimmune women: What is the optimal pregnancy management?

J Clin Virol 2018 Nov 2;108:141-146. Epub 2018 Oct 2.

Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Section of Microbiology, Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. Electronic address:

Background: Human cytomegalovirus (HCMV) infection is the most common congenital infection in developed countries. Recent studies highlighted similar percentages of symptoms in HCMV congenitally-infected infants following either primary or non-primary maternal infections.

Objectives: To highlight correlation between neonatal brain abnormalities, detected by ultrasounds and magnetic resonance image in HCMV congenitally-infected infants, and maternal virological parameters during pregnancy, especially in seroimmune mothers. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.10.001DOI Listing
November 2018
2 Reads

Biological relevance of Cytomegalovirus genetic variability in congenitally and postnatally infected children.

J Clin Virol 2018 Nov 2;108:132-140. Epub 2018 Oct 2.

Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. Electronic address:

Background: Human cytomegalovirus (HCMV) is the leading cause of congenital infections resulting in severe morbidity and mortality among infected children. Although the virus is highly polymorphic, particularly in genes contributing to immune evasion, the mechanisms underlying its genetic variability and pathogenicity are only partially understood.

Objectives: We aimed to characterize different HCMV clinical strains isolated from 21 congenitally- or postnatally-infected children for in vitro growth properties and genetic polymorphisms. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.019DOI Listing
November 2018
2 Reads

CD4 T cell responses to flaviviruses.

J Clin Virol 2018 Nov 3;108:126-131. Epub 2018 Oct 3.

Center for Virology, Medical University of Vienna, Vienna, Austria.

Flaviviruses pose an increasing threat to global health with their potential to cause severe disease in millions of people. Protective and long-lived immunity is closely linked to the generation of CD4 T cells, which provide B cell help and support high affinity neutralizing antibody responses. Research performed during the last years revealed important new insights into the antigen specificities and diverse effector functions of CD4 T cell responses to flaviviruses. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.020DOI Listing
November 2018

Recognition, treatment, and sequelae of congenital cytomegalovirus in Australia: An observational study.

J Clin Virol 2018 Nov 27;108:121-125. Epub 2018 Sep 27.

School of Women's and Children's Health, University of New South Wales, Sydney, Australia; Serology & Virology Division, SEALS Microbiology, Prince of Wales Hospital, Sydney, Australia; School of Medical Sciences, University of New South Wales, Sydney, Australia; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia. Electronic address:

Background And Objectives: Australian national surveillance data was used to assess recognition, sequelae, and antiviral therapy for congenital cytomegalovirus (CMV) cases.

Study Design: Data from congenital CMV cases reported through the Australian Paediatric Surveillance Unit born January 1999 to December 2016 were described and Chi-square tests used to characterise trends and associations in case reporting, maternal CMV serology testing, and antiviral therapy. Descriptive analyses for hearing loss and developmental delay were reported for cases born ≥2004, following introduction of universal neonatal hearing screening. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.017DOI Listing
November 2018
2 Reads

Virome definition in cerebrospinal fluid of patients with neurological complications after hematopoietic stem cell transplantation.

J Clin Virol 2018 Nov 30;108:112-120. Epub 2018 Sep 30.

Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden. Electronic address:

Background: Neurological complications (NC) in allogeneic hematopoietic stem cell transplant (HSCT) recipients lead to long-term sequelae and result in significant morbidity and mortality. Since risk factors for NC include viral infection or reactivation, virome inspection after HSCT might be helpful to the clinical management of patients after HSCT.

Objectives And Study Design: In this study we investigated whether any viruses are found in association with NC after HSCT. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183024
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http://dx.doi.org/10.1016/j.jcv.2018.09.014DOI Listing
November 2018
2 Reads

Differences in the ease with which mutant viruses escape from human monoclonal antibodies against the HA stem of influenza A virus.

J Clin Virol 2018 Nov 26;108:105-111. Epub 2018 Sep 26.

Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Japan; Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Japan; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, USA. Electronic address:

Background: Broadly protective human monoclonal antibodies that recognize the conserved epitopes in the HA of influenza A virus are being developed as therapeutic agents. Emergence of resistant viruses must always be considered when developing therapeutic agents against influenza.

Objectives: We examined human hetero-reactive mAbs against the HA stem of influenza A virus for the ease with which escape mutant viruses emerged. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.016DOI Listing
November 2018
2 Reads

Is active hepatitis C associated with the size of HIV reservoir in successfully treated patients?

J Clin Virol 2018 Nov 27;108:103-104. Epub 2018 Sep 27.

Virology Laboratory Unit, L'Archet Hospital, University of Nice, France.

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http://dx.doi.org/10.1016/j.jcv.2018.09.018DOI Listing
November 2018
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Within-host evolution of virus variants during chronic infection with novel GII.P26-GII.26 norovirus.

J Clin Virol 2018 Nov 19;108:96-102. Epub 2018 Sep 19.

Institute of Virology, Leipzig University, Leipzig, Germany. Electronic address:

Background: Noroviruses are a leading cause of acute gastroenteritis in all age groups. They generally cause a rapidly self-limiting illness. However, chronic norovirus diarrheal disease occurs in immunocompromised individuals, and is accompanied by persistent shedding of infectious norovirus in stool. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183024
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http://dx.doi.org/10.1016/j.jcv.2018.09.013DOI Listing
November 2018
12 Reads

Clinical impact of rapid molecular detection of respiratory pathogens in patients with acute respiratory infection.

J Clin Virol 2018 Nov 14;108:90-95. Epub 2018 Sep 14.

Clinical Virology Unit, Centro de Educación Médica e Investigaciones Clínicas "CEMIC" - Consejo Nacional de Investigaciones Científicas y Técnicas "CONICET", Av. Galván 4102 (1431), Buenos Aires City, Argentina.

Background: Acute respiratory infections (ARI) are a leading cause of morbidity and mortality worldwide. There is a need to demonstrate the clinical impact of using the new, rapid and sensitive molecular assays in prospectively designed studies.

Objectives: To study the impact on medical management of a rapid molecular assay in patients with respiratory infections. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532183022
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http://dx.doi.org/10.1016/j.jcv.2018.09.009DOI Listing
November 2018
10 Reads

PCR assays for detection of human astroviruses: In silico evaluation and design, and in vitro application to samples collected from patients in the Netherlands.

J Clin Virol 2018 Nov 13;108:83-89. Epub 2018 Sep 13.

Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia. Electronic address:

Background: Human astroviruses (HAstV) comprise three phylogenetically compact and non-adjacent groups of species including classical HAstV (HAstV-C) and the novel ones (HAstV-VA/HMO and HAstV-MLB). Of these, HAstV-C is known to be responsible for gastroenteritis while the novel HAstV are associated with cases of neurological disorders. Accurate detection of all known variants by (real-time) PCR is challenging because of the high intra- and intergroup genetic divergence of HAstV. Read More

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http://dx.doi.org/10.1016/j.jcv.2018.09.007DOI Listing
November 2018
1 Read