2,567 results match your criteria Journal of Clinical Laboratory Analysis[Journal]


Increased serum adiponectin predicts improved coronary flow and clinical outcomes in patients with ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention.

J Clin Lab Anal 2019 Feb 19:e22864. Epub 2019 Feb 19.

Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with ST-segment elevation myocardial infarction (STEMI) is unclear.

Methods: A total of 144 patients with STEMI treated by PPCI were enrolled and divided into two groups based on the mean serum APN level on admission. Read More

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http://dx.doi.org/10.1002/jcla.22864DOI Listing
February 2019

Upregulation of miR-146a-5p is associated with increased proliferation and migration of vascular smooth muscle cells in aortic dissection.

J Clin Lab Anal 2019 Feb 19:e22843. Epub 2019 Feb 19.

Guangdong Cardiovascular Institute, Guangdong Provincial People' Hospital, Guangdong Academy of Medical Sciences, Guangdong, Guangzhou, China.

Background: This study aimed to investigate whether miR-146a-5p was involved in the pathogenesis of thoracic aortic dissection (AD) via regulating the biological function of vascular smooth muscle cells (VSMCs).

Methods: Circulating miR-146a-5p level was measured by quantitative polymerase chain reaction (qPCR) in AD patients and healthy controls. Human dissected aortic samples were obtained from patients with thoracic AD Stanford type A undergoing surgical repair, and normal control samples were from organ donors who died from nonvascular diseases. Read More

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http://dx.doi.org/10.1002/jcla.22843DOI Listing
February 2019

Distribution of serum neuron-specific enolase and the establishment of a population reference interval in healthy adults.

J Clin Lab Anal 2019 Feb 19:e22863. Epub 2019 Feb 19.

Department of Laboratory Medicine, The Second People's Hospital of Lianyungang, Lianyungang, China.

Background: Neuron-specific enolase (NSE) is an important tumor marker in the serum of patients with lung cancer. Elevated serum NSE levels are also associated with many other diseases. However, there is no unified population reference interval for serum NSE. Read More

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http://dx.doi.org/10.1002/jcla.22863DOI Listing
February 2019

Determination of hemolysis index thresholds for biochemical tests on Siemens Advia 2400 chemistry analyzer.

J Clin Lab Anal 2019 Feb 19:e22856. Epub 2019 Feb 19.

Department of clinical laboratory, Characteristic Medical center of Chinese People's Armed Police Force, Pingjin Hospital, Tianjin, China.

Background: In vitro hemolysis is still the most common source of pre-analytical nonconformities. This study aimed to investigate the hemolytic effects on commonly used biochemical tests as well as to determine the hemolysis index (HI) thresholds on Siemens Advia 2400 chemistry analyzer.

Methods: Peripheral blood samples were collected from forty healthy volunteers. Read More

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http://dx.doi.org/10.1002/jcla.22856DOI Listing
February 2019

The clinical value of hematological markers in rheumatoid arthritis patients treated with tocilizumab.

J Clin Lab Anal 2019 Feb 19:e22862. Epub 2019 Feb 19.

Department of Preventive Medicine, Medical School of Ningbo University, Ningbo, China.

Background: Emerging evidence indicates that some hematological markers have critical value in evaluating treatment response. This study was performed to determine the clinical value of hemoglobin (Hb), platelet (Plt), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in rheumatoid arthritis (RA) patients treated with tocilizumab (TCZ).

Methods: Fifty-two RA patients receiving TCZ were recruited and followed for 6 months. Read More

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http://dx.doi.org/10.1002/jcla.22862DOI Listing
February 2019

Intra- and inter-laboratory agreement of the FAM19A4/mir124-2 methylation test: Results from an international study.

J Clin Lab Anal 2019 Feb 13:e22854. Epub 2019 Feb 13.

Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, Amsterdam, the Netherlands.

Background: HPV-based cervical screening detects women at an increased risk of cervical cancer and precancer. To differentiate among HPV-positive women those with (pre)cancer, triage testing is necessary. The detection of cancer-associated host-cell DNA methylation (FAM19A4 and hsa-mir124-2) in cervical samples has shown valuable as triage test. Read More

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http://dx.doi.org/10.1002/jcla.22854DOI Listing
February 2019

Establishment of a bead-based duplex assay for the simultaneous quantitative detection of Neuropilin-1 and Neuropilin-2 using xMAP technology and its clinical application.

J Clin Lab Anal 2019 Feb 13:e22850. Epub 2019 Feb 13.

Cancer Research Center, Medical College, Xiamen University, Xiamen, China.

Background: Neuropilins (Nrps) are a new type of broad-spectrum tumor marker. Currently, a method for accurate simultaneous quantification of Nrps is not available. We aimed to develop a bead-based and duplexed flow cytometric assay that could be used for accurate and simultaneous quantification of Nrp1 and Nrp2 for scientific research or clinical diagnosis. Read More

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http://dx.doi.org/10.1002/jcla.22850DOI Listing
February 2019

Predictive value of soluble fms-like tyrosine kinase-1 against placental growth factor for preeclampsia in a Chinese pregnant women population.

J Clin Lab Anal 2019 Feb 13:e22861. Epub 2019 Feb 13.

Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu, China.

Objective: The purpose of the present study was to explore the predictive effects of soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) for preeclampsia.

Methods: A total of 1580 singleton pregnant women aged 18-45 years were included in this study. Serum samples were collected and stored frozen during their regular obstetric examinations. Read More

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http://doi.wiley.com/10.1002/jcla.22861
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http://dx.doi.org/10.1002/jcla.22861DOI Listing
February 2019
5 Reads

Interleukin-21 receptor gene polymorphism is associated with hepatitis B virus-related hepatocellular carcinoma in Chinese patients.

J Clin Lab Anal 2019 Feb 13:e22860. Epub 2019 Feb 13.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chendu, Sichuan, China.

Background: We investigated the relationship between hepatitis B virus (HBV)-related pathogenesis and single nucleotide polymorphisms (SNPs) in interleukin-21 (IL-21)-JAK-STAT signaling pathway genes.

Methods: We used the high-resolution melting (HRM) method to genotype five SNPs (IL-21 rs2221903, IL-21 rs4833837, IL-21 receptor (IL-21R) rs2285452, JAK3 rs3008, and STAT3 rs1053023) in 546 HBV-infected patients and 353 healthy Chinese subjects. The HBV-infected patients were further divided into subgroups based on the HBV-related pathologies: chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC), and HBV-related hepatocellular carcinoma (HCC). Read More

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http://dx.doi.org/10.1002/jcla.22860DOI Listing
February 2019

Detection of the major bacterial pathogens among children suffering from empyema in Ahvaz city, Iran.

J Clin Lab Anal 2019 Feb 9:e22855. Epub 2019 Feb 9.

Department of microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Introduction: Empyema is one of the important causes of pediatric hospital admissions.

Aim: In this study, we had investigated the frequency rates of S aureus, S pneumoniae, H influenzae, and P aeruginosa using PCR and bacterial culture among children suffering from empyema in Ahvaz city, Iran.

Methods: This was a descriptive study conducted on the patients hospitalized in ICUs of two teaching Hospitals of Ahvaz, Iran, between March and September 2018 on 105 pleural fluid (PF) samples of the children less than 16 years of age with the diagnosis of empyema thoracis. Read More

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http://dx.doi.org/10.1002/jcla.22855DOI Listing
February 2019
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Prevalence of pks gene cluster and characteristics of Klebsiella pneumoniae-induced bloodstream infections.

J Clin Lab Anal 2019 Feb 8:e22838. Epub 2019 Feb 8.

Department of Clinical Laboratory, Xiangya Hospital Central South University, Changsha, China.

Background: The emerging pks-positive (pks ) strains have aroused great public concern recently. Colibactin, encoded by pks gene cluster, has been reported to be involved in DNA damage and increased virulence. Little is known about its prevalence among Klebsiella pneumoniae-induced bloodstream infections (BSIs). Read More

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http://dx.doi.org/10.1002/jcla.22838DOI Listing
February 2019

Neutrophil-to-lymphocyte ratio can specifically predict the severity of hypertriglyceridemia-induced acute pancreatitis compared with white blood cell.

J Clin Lab Anal 2019 Feb 8:e22839. Epub 2019 Feb 8.

Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: We aimed to evaluate the values of neutrophil-to-lymphocyte ratio (NLR) and white blood cell (WBC) in predicting severity of acute pancreatitis (AP) with different etiologies.

Methods: We compared NLR and WBC levels in patients with different etiologies and AP severity. The optimal cutoff value for them to predict severe acute pancreatitis (SAP) was determined by receiver operating characteristic (ROC) curve analysis. Read More

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http://dx.doi.org/10.1002/jcla.22839DOI Listing
February 2019
1 Read

Iodine status of euthyroid adults: A cross-sectional, multicenter study.

J Clin Lab Anal 2019 Feb 8:e22837. Epub 2019 Feb 8.

Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China.

Background: Iodine, an essential nutrient, is the most important trace element in thyroid hormone synthesis and maintenance of thyroid function. This study investigated the iodine nutrition status in healthy Chinese adults and assessed the relationship between urinary iodine concentration (UIC) and thyroid hormone levels.

Methods: A cross-sectional, multicenter study was conducted between October 2017 and January 2018, with 1017 adults recruited from five cities in China. Read More

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http://dx.doi.org/10.1002/jcla.22837DOI Listing
February 2019
1 Read

The association between CRP / Albumin ratio and in-stent restenosis development in patients with ST-segment elevation myocardial infarction.

J Clin Lab Anal 2019 Feb 8:e22848. Epub 2019 Feb 8.

Department of Cardiology, Erzurum Training and Research Hospital, Erzurum, Turkey.

Background: Although interventional methods are the first-line treatment options in ST-segment elevation myocardial infarction (STEMI), the incidence of stent restenosis (SR) is frequent. We investigated the relationship between CRP/albumin ratio (CAR), a novel indicator of inflammatory response, and SR in this study.

Method: This study was carried out on the patients who underwent coronary angiography in our clinic between January 2017 and September 2017. Read More

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http://dx.doi.org/10.1002/jcla.22848DOI Listing
February 2019
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FLT3, a prognostic biomarker for acute myeloid leukemia (AML): Quantitative monitoring with a simple anti-FLT3 interaction and flow cytometric method.

J Clin Lab Anal 2019 Feb 8:e22859. Epub 2019 Feb 8.

Center for Research and Development of Natural Products for Health, Chiang Mai University, Chiang Mai, Thailand.

Background: Overexpression of fms-like tyrosine kinase 3 (FLT3) protein in leukemia is highly related to poor prognosis and reduced survival rate in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) patients. Simple but efficient quantification of FLT3 protein levels on the leukemic cell surface using flow cytometry had been developed for rapid determination of FLT3 on intact cell surface.

Methods: Quantitation protocol for FLT3 biomarker in clinical samples was developed and validated. Read More

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http://dx.doi.org/10.1002/jcla.22859DOI Listing
February 2019
2 Reads

Clinical significance of cancer-associated fibroblasts and their correlation with microvessel and lymphatic vessel density in lung adenocarcinoma.

J Clin Lab Anal 2019 Feb 8:e22832. Epub 2019 Feb 8.

Department of Oncology, Qingdao Municipal Hospital, Qingdao, China.

Background: To determine whether cancer-associated fibroblasts (CAFs) are associated with microvessel density (MVD) and lymphatic vessel density (LVD) in lung adenocarcinoma (ADC) or are not prognostic.

Methods: Ninety-three lung adenocarcinoma patients without adjuvant therapy between January 2010 and June 2011 were enrolled. CAFs, MVD, and LVD were identified by α-smooth muscle actin (α-SMA), CD34 and D2-40 staining via immunohistochemistry. Read More

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http://dx.doi.org/10.1002/jcla.22832DOI Listing
February 2019
2 Reads

How useful is bone marrow study as an initial investigative tool without lymph node biopsy in malignant lymphoma?: Eleven years of experience at a single institution.

J Clin Lab Anal 2019 Feb 6:e22841. Epub 2019 Feb 6.

Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Korea.

Background: Bone marrow (BM) study plays an important role as initial investigation specimen of lymphoma as well as staging lymphoma. This study aimed to investigate the utility of BM studies for classification of lymphoma and evaluate features of BM involvement by lymphoma over a period of 11 years.

Methods: A total of 1162 cases of BM studies for lymphoma evaluation were reviewed for the incidence of lymphoma subtypes, the percentage of marrow involvement, the pattern of involvement and discordance with histopathologic diagnoses of lymph nodes and other tissues. Read More

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http://dx.doi.org/10.1002/jcla.22841DOI Listing
February 2019
1 Read

Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study.

J Clin Lab Anal 2019 Feb 4:e22842. Epub 2019 Feb 4.

Department of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, Suzhou, Jiangsu, China.

Background: Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce.

Methods: Fifty incident T2DM cases and 100 matched non-T2DM controls were selected from a prospective cohort study of "135." The composition of the blood microbiome was characterized using bacterial 16S ribosomal RNA (16S rRNA) gene sequencing from pre-diagnostic blood sample. Read More

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http://dx.doi.org/10.1002/jcla.22842DOI Listing
February 2019
2 Reads

Maternal peripheral blood platelet-to-white blood cell ratio and platelet count as potential diagnostic markers of histological chorioamnionitis-related spontaneous preterm birth.

J Clin Lab Anal 2019 Feb 4:e22840. Epub 2019 Feb 4.

Obstetrical Department, Fujian Provincial Maternity and Children's Hospital, affiliated Hospital of Fujian Medical University, Fujian, China.

Background: Histological chorioamnionitis (HCA) is one of the leading causes of spontaneous preterm birth, thus, to identify novel biomarkers for the early diagnosis of HCA is in a great need.

Objective: To investigate the diagnostic value of maternal peripheral blood platelet-to-white blood cell ratio (PLT/WBC) and platelet (PLT) counts in HCA-related preterm birth.

Methods: A total of 400 patients with preterm birth were enrolled in this study: non-HCA group (n = 193) and HCA group (n = 207), and 87 full-term pregnancies were enrolled as the control. Read More

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http://dx.doi.org/10.1002/jcla.22840DOI Listing
February 2019

Clinical features and disease severity of Turkish FMF children carrying E148Q mutation.

J Clin Lab Anal 2019 Feb 4:e22852. Epub 2019 Feb 4.

Department of Pediatric Rheumatology and Nephrology, Ankara University School of Medicine, Ankara, Turkey.

Background: Familial Mediterranean fever (FMF) is the most common hereditary monogenic autoinflammatory disease caused by mutations in the MEFV gene. It is controversial whether E148Q alteration is an insignificant variant or a disease-causing mutation. The aim of this study was to evaluate the clinical features and disease severity of FMF patients carrying E148Q mutation. Read More

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http://dx.doi.org/10.1002/jcla.22852DOI Listing
February 2019
2 Reads

Vitamin D levels and vitamin D receptor variants are associated with chronic heart failure in Chinese patients.

Authors:
Yi Hao Yueling Chen

J Clin Lab Anal 2019 Feb 4:e22847. Epub 2019 Feb 4.

Department of Cardiothoracic Surgery, Beijing Luhe Hospital of Capital Medical University, Beijing, China.

Background: Vitamin D is an indispensable molecule for human health. Wide ranges of diseases are linked with vitamin D deficiencies. Role of vitamin D in chronic heart failure has been demonstrated in different populations; however, reports are limited in Chinese population. Read More

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http://dx.doi.org/10.1002/jcla.22847DOI Listing
February 2019
1 Read

Association of Crohn's disease with Foxp3 gene polymorphisms and its colonic expression in Chinese patients.

J Clin Lab Anal 2019 Feb 1:e22835. Epub 2019 Feb 1.

Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Background: Fork head/winged helix transcription factor (Foxp3) plays a pivotal role in regulatory T (Treg) cells. The present study aimed to assess the association of Crohn's disease (CD) with Foxp3 polymorphisms and its colonic expression in Chinese patients.

Methods: The Foxp3 polymorphisms, rs3761547, rs2232365, rs2294021, and rs3761548, were examined by SNaPshot in 268 CD patients and 490 controls. Read More

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http://dx.doi.org/10.1002/jcla.22835DOI Listing
February 2019
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Correlations between serum amyloid A, C-reactive protein and clinical indices of patients with acutely exacerbated chronic obstructive pulmonary disease.

J Clin Lab Anal 2019 Jan 21:e22831. Epub 2019 Jan 21.

Department of Critical Care Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, China.

Background: To explore the correlations between SAA, CRP, and clinical indices of patients with acutely exacerbated chronic obstructive pulmonary disease (AECOPD).

Methods: A total of 120 patients with AECOPD and another 120 with remitted COPD were enrolled in an AECOPD group and a COPD remission group, respectively. Meanwhile, 120 healthy subjects were included as a control group. Read More

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http://doi.wiley.com/10.1002/jcla.22831
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http://dx.doi.org/10.1002/jcla.22831DOI Listing
January 2019
3 Reads

Diagnostic value of derived neutrophil-to-lymphocyte ratio in patients with ovarian cancer.

J Clin Lab Anal 2019 Jan 21:e22833. Epub 2019 Jan 21.

Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Inflammation plays an important role in the occurrence and development of cancer. Numerous studies have used the derived neutrophil-to-lymphocyte ratio (dNLR) to evaluate prognosis in many types of cancer. However, the relationship between dNLR and ovarian cancer and its value in the differential diagnosis of benign and malignant ovarian tumors remain unknown. Read More

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http://doi.wiley.com/10.1002/jcla.22833
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http://dx.doi.org/10.1002/jcla.22833DOI Listing
January 2019
4 Reads

Seasonal influence on TORCH infection and analysis of multi-positive samples with indirect immunofluorescence assay.

J Clin Lab Anal 2019 Jan 21:e22828. Epub 2019 Jan 21.

Department of Laboratory Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Background: TORCH including the pathogens of Toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV), and herpes simplex virus (HSV) causes intrauterine infections and poses a worldwide threat to women especially in pregnancy. In this study, we described the seasonal difference in TORCH infection and analyzed the anti-TORCH IgM multipositive serum samples by the indirect immunofluorescence assays (IFA).

Methods: To observe the seasonal influence of the anti-TORCH IgG and IgM antibodies, a retrospective study was conducted with 10 669 women (20-40 y old) before pregnancy from August 2016 to July 2017. Read More

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http://doi.wiley.com/10.1002/jcla.22828
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http://dx.doi.org/10.1002/jcla.22828DOI Listing
January 2019
3 Reads

Association of pro- and anti-inflammatory cytokines in preeclampsia.

J Clin Lab Anal 2019 Jan 21:e22834. Epub 2019 Jan 21.

Department of Anatomy, Vardhman Mahavir Medical College & Safdarjung Hospital, New Delhi, India.

Introduction: The pro- and anti-inflammatory cytokines play crucial role in the development and functions of placenta. Any changes in these cytokines may be associated with many pregnancy-related disorders like preeclampsia. Therefore, the present study is aimed to study the expression of pro-inflammatory (TNF-α, IL-6) and anti-inflammatory (IL-4, IL-10) cytokines in placenta and serum of preeclamptic pregnant women. Read More

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http://dx.doi.org/10.1002/jcla.22834DOI Listing
January 2019
5 Reads

Detection of 21q11.2-q22.11 deletions in a fetus by NIPT.

J Clin Lab Anal 2019 Jan 21:e22711. Epub 2019 Jan 21.

Department of Obstetrics and Gynecology, The First Affiliated Hospital Of Air Force Medical University, Xi'an, Shaanxi, China.

Background: Non-invasive prenatal testing (NIPT) is extensively used in the detection of fetal trisomies 21, 18, and 13, which is promptly becoming a common clinical practice. Concerned about the clinical application of non-invasive detection of the fetal autosomal duplications or deletion.

Case Presentation: A 34-year-old, healthy pregnant woman was referred to the First Affiliated Hospital of the Air Force Medical University. Read More

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http://doi.wiley.com/10.1002/jcla.22711
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http://dx.doi.org/10.1002/jcla.22711DOI Listing
January 2019
6 Reads

Development and validation of an advanced fragment analysis-based assay for the detection of 22 pathogens in the cerebrospinal fluid of patients with meningitis and encephalitis.

J Clin Lab Anal 2019 Jan 21:e22707. Epub 2019 Jan 21.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Background: Meningitis and encephalitis (ME) are central nervous system (CNS) infections mainly caused by bacteria, mycobacteria, fungi, viruses, and parasites that result in high morbidity and mortality. The early, accurate diagnosis of pathogens in the cerebrospinal fluid (CSF) and timely medication are associated with better prognosis. Conventional methods, such as culture, microscopic examination, serological detection, CSF routine analysis, and radiological findings, either are time-consuming or lack sensitivity and specificity. Read More

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http://dx.doi.org/10.1002/jcla.22707DOI Listing
January 2019
1 Read

Determination of vitamin D in tears of healthy individuals by the electrochemiluminescence method.

J Clin Lab Anal 2019 Jan 21:e22830. Epub 2019 Jan 21.

Laboratório de Análises Clínicas da Faculdade de Medicina do ABC, Santo André, Brazil.

Background: Vitamin D is a fat-soluble steroid hormone which can be converted into various forms and is of extreme physiological importance to our body. However, its functions and local metabolic pathways in some organs, such as the eye, have not yet been well studied. We aimed to verify the correlation between vitamin D levels in blood and tear fluid and the possibility of using tear fluid as a biological material for monitoring eye disorders in the future. Read More

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http://doi.wiley.com/10.1002/jcla.22830
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http://dx.doi.org/10.1002/jcla.22830DOI Listing
January 2019
2 Reads

Comprehensive analysis of presurgical factors predicting psychiatric disorders in patients with refractory temporal lobe epilepsy and mesial temporal sclerosis underwent cortico-amygdalohippocampectomy.

J Clin Lab Anal 2018 Dec 5:e22724. Epub 2018 Dec 5.

Department of Neurosurgery, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Chengdu, China.

Background: This study aimed to evaluate the predictive value of presurgical factors for psychiatric disorders (PD) in refractory temporal lobe epilepsy and mesial temporal sclerosis (TLE-MTS) patients underwent cortico-amygdalohippocampectomy (CAH).

Methods: A total of 98 refractory TLE-MTS patients underwent CAH were consecutively enrolled in this cohort study. Several presurgical factors were recorded, such as married status, employment status, highest education, disease duration, family history of epilepsy, and disorganized VEEG background activity. Read More

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http://dx.doi.org/10.1002/jcla.22724DOI Listing
December 2018
1 Read
1.144 Impact Factor

Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure.

J Clin Lab Anal 2018 Nov 29:e22713. Epub 2018 Nov 29.

Department of Blood Purification, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Chronic renal failure (CRF) has become a major public health concern, which increases the risk of stroke and systemic thromboembolism. Therefore, therapeutic strategies are in urgent requirement. This study was conducted for investigating efficacy of hemodialysis (HD), hemodiafiltration (HDF), and hemoperfusion (HP) in patients with CRF and the correlation with the presence of complications following HD therapy. Read More

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http://dx.doi.org/10.1002/jcla.22713DOI Listing
November 2018

Analytical validation of an in-house method for adenosine deaminase determination.

J Clin Lab Anal 2018 Nov 29:e22823. Epub 2018 Nov 29.

Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.

Background: The adenosine deaminase (ADA) enzyme is a marker of inflammatory processes whose activity can be measured through a colorimetric method developed as an in-house assay. This validation can reduce costs and expand the alternatives for laboratory diagnosis.

Methods: The ADA analysis was achieved through a modified form of Giusti and Galanti's (1984) method. Read More

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http://dx.doi.org/10.1002/jcla.22823DOI Listing
November 2018
1 Read

Serum markers in the differential diagnosis of Waldenstrom macroglobulinemia and other IgM monoclonal gammopathies.

J Clin Lab Anal 2018 Nov 28:e22827. Epub 2018 Nov 28.

The Central Laboratory, Fujian Medical University Union Hospital, Fuzhou, China.

Background: IgM monoclonal gammopathy can be present in a broad spectrum of diseases. We evaluated the value of serum markers in the differential diagnosis of Waldenstrom macroglobulinemia (WM) and other types of IgM monoclonal gammopathies.

Methods: We included patients who were first admitted to hospital and identified as having IgM monoclonal gammopathy by serum immunofixation electrophoresis (sIFE). Read More

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http://dx.doi.org/10.1002/jcla.22827DOI Listing
November 2018
4 Reads

Altered expression of circular RNAs in human placental chorionic plate-derived mesenchymal stem cells pretreated with hypoxia.

J Clin Lab Anal 2018 Nov 28:e22825. Epub 2018 Nov 28.

Guangzhou Women and Children's Medical Center, Sun Yat-sen University, Guangzhou, China.

Background: Hypoxic preconditioning alters the biological properties of mesenchymal stem cells (MSCs). It is not known whether this process has an effect on circular RNAs (circRNAs) in MSCs.

Methods: Human placental chorionic plate-derived MSCs (hpcpMSCs) isolated from the same placentae were classed into two groups: hypoxic pretreated (hypoxia) group and normally cultured (normoxia) group. Read More

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http://dx.doi.org/10.1002/jcla.22825DOI Listing
November 2018
1 Read
1.144 Impact Factor

Assessment of rapid diagnostic tests and fourth-generation Enzyme-Linked Immunosorbent Assays in the screening of Human Immunodeficiency and Hepatitis B virus infections among first-time blood donors in Libreville (Gabon).

J Clin Lab Anal 2018 Nov 28:e22824. Epub 2018 Nov 28.

Laboratoire de Biologie Moléculaire et Cellulaire, Université des Sciences et Techniques de Masuku, Franceville, Gabon.

Background: Blood transfusion is a pathway for the transmission of blood-borne pathogens such as human immunodeficiency virus (HIV) and hepatitis B virus (HBV) from donors to recipients in most countries in sub-Saharan Africa, including Gabon. The study aimed to compare the performance of four rapid diagnostic tests (RDTs: Alere DETERMINE, BIOSYNEX Exacto Pro HIV, MEDIFF HIV 1&2, and BIOSYNEX IMMUNOQUICK HBsAg) with results of 4th-generation immunoenzymatic assays COBAS 6000 e601 and EVOLIS BioRad for the detection of HIV and hepatitis B surface antigen (HBsAg) in blood donors in Libreville, Gabon.

Methods: Reactive and nonreactive blood samples for HIV and HBsAg were selected using fourth-generation ELISA COBAS 6000 e601 and EVOLIS BioRad. Read More

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http://dx.doi.org/10.1002/jcla.22824DOI Listing
November 2018
4 Reads
1.144 Impact Factor

Use of the Sysmex XT-4000i hematology analyzer in the differentiation of cerebrospinal fluid cells in children.

J Clin Lab Anal 2018 Nov 28:e22822. Epub 2018 Nov 28.

Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland.

Background: Routine analysis of pleocytosis and cellular composition of cerebrospinal fluid (CSF) is carried out with a phase-contrast microscope. The use of hematological analyzers seems to be an alternative to the manual method. The aim of the study was to assess the usefulness of the automated technique for counting and differentiating CSF cells in children. Read More

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http://doi.wiley.com/10.1002/jcla.22822
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http://dx.doi.org/10.1002/jcla.22822DOI Listing
November 2018
1 Read

Parathyroidectomy decreases serum intact parathyroid hormone and calcium levels and prolongs overall survival in elderly hemodialysis patients with severe secondary hyperparathyroidism.

J Clin Lab Anal 2018 Nov 28:e22696. Epub 2018 Nov 28.

Department of Thyroid Breast Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The objective of this study was to assess the effect of parathyroidectomy (PTX) treatment on prolonging overall survival (OS) as well as decreasing levels of intact parathyroid hormone (iPTH), calcium (Ca), and phosphorus (P) in elderly hemodialysis patients with severe secondary hyperparathyroidism (SHPT).

Methods: A total of 304 elderly hemodialysis patients with severe SHPT were consecutively enrolled in this cohort study. According to whether PTX operations were applied, patients were classified into PTX group (N = 112) and Control group (N = 192) and were followed up for 3 years. Read More

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http://dx.doi.org/10.1002/jcla.22696DOI Listing
November 2018
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Association of the study between LncRNA-H19 gene polymorphisms with the risk of breast cancer.

J Clin Lab Anal 2018 Nov 28:e22826. Epub 2018 Nov 28.

Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran.

Background: The H19 is a maternally expressed imprinted gene transcribing a long noncoding RNA (lncRNA), which has previously been reported to be involved in tumorigenesis and cancer progression. The aim of this study was to evaluate the associations between two lncRNA-H19 (rs3741219 T>C and rs217727 C>T) gene polymorphisms with the risk of breast cancer (BC).

Methods: In a case-control investigation, we evaluated 150 BC patients and 100 cancer-free subjects in East Azerbaijan Province of Iran. Read More

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http://dx.doi.org/10.1002/jcla.22826DOI Listing
November 2018
8 Reads
1.144 Impact Factor

Response to the "Letter to the editor regarding article, 'High-mobility group box 1 as a biomarker in critically ill patients'."

J Clin Lab Anal 2018 Nov 25:e22820. Epub 2018 Nov 25.

Department of Medicine III, RWTH-University Hospital Aachen, Aachen, Germany.

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http://dx.doi.org/10.1002/jcla.22820DOI Listing
November 2018
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Importance of sample volume to the measurement and interpretation of plasma osmolality.

J Clin Lab Anal 2018 Nov 25:e22727. Epub 2018 Nov 25.

U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts.

Background: Small sample volumes may artificially elevate plasma osmolality (Posm) measured by freezing point depression. The purpose of this study was to compare two widely different sample volumes of measured Posm (mmol/kg) to each other, and to calculated osmolarity (mmol/L), across a physiological Posm range (~50 mmol/kg).

Methods: Posm was measured using freezing point depression and osmolarity calculated from measures of sodium, glucose, and blood urea nitrogen. Read More

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http://doi.wiley.com/10.1002/jcla.22727
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http://dx.doi.org/10.1002/jcla.22727DOI Listing
November 2018
11 Reads

Letter to the Editor Regarding Article, "High mobility group box 1 as a biomarker in critically ill patients".

J Clin Lab Anal 2018 Nov 25:e22821. Epub 2018 Nov 25.

Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University and Guangxi Key Laboratory Base of Precision Medicine in Cardio-cerebrovascular Diseases Control and Prevention and Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, Nanning, China.

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http://doi.wiley.com/10.1002/jcla.22821
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http://dx.doi.org/10.1002/jcla.22821DOI Listing
November 2018
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Combined testing for herpes simplex virus and Mycobacterium tuberculosis DNA in cerebrospinal fluid of patients with aseptic meningitis in Burkina Faso, West Africa.

J Clin Lab Anal 2018 Nov 25:e22719. Epub 2018 Nov 25.

UMR 1058, INSERM/EFS/Université de Montpellier, Montpellier, France.

Background: Little is known about the involvement of herpes simplex virus (HSV) or Mycobacterium tuberculosis (MTB) as potentially curable causes of central nervous system (CNS) infections in sub-Saharan Africa.

Objective: In this study, we developed a PCR assay dedicated to simultaneous testing of HSV1/HSV2 and MTB in Burkina Faso, a country where HSV is neglected as a cause of CNS infection and where TB prevalence is high.

Methods: A consensus HSV1/HSV2 set of primers and probe were designed and combined to primers and probe targeting the IS6110 repetitive insertion sequence of MTB. Read More

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http://dx.doi.org/10.1002/jcla.22719DOI Listing
November 2018
2 Reads

Hsa_circ_0000467 promotes cancer progression and serves as a diagnostic and prognostic biomarker for gastric cancer.

J Clin Lab Anal 2018 Nov 21:e22726. Epub 2018 Nov 21.

Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, China.

Background: Emerging evidence indicates that dysregulation of circular RNAs (circRNAs) is implicated in the development of malignancies. However, the diagnostic value and functional role of circRNAs in gastric cancer (GC) remain largely elusive.

Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to detect the expression of hsa_circ_0000467 in tissues, cell lines, and plasma. Read More

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http://dx.doi.org/10.1002/jcla.22726DOI Listing
November 2018
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Insignificant interference of Elevit in pregnant women serum samples with HBsAg immunoassay on Sysmex.

J Clin Lab Anal 2018 Nov 21:e22725. Epub 2018 Nov 21.

Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu, China.

Background: The pregnant women often take Elevit as the multivitamin supplement which has a substantial amount of biotin that might potentially interfere with the HBsAg immunoassay performed by the prevalent Sysmex system in clinical laboratories. We therefore wanted to determine this, so that the therapeutic intervention on the hepatitis B virus infection during pregnancy and birth would not be missed.

Methods: Elevit was both serially diluted in vitro and orally taken by healthy volunteers whose blood samples were then taken at different time points. Read More

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http://dx.doi.org/10.1002/jcla.22725DOI Listing
November 2018
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Circulating CEA-dNLR score predicts clinical outcome of metastatic gallbladder cancer patient.

Authors:
Jing-Hui Du Jun Lu

J Clin Lab Anal 2019 Feb 21;33(2):e22684. Epub 2018 Nov 21.

Department of Clinical Laboratory, Longyan People Hospital, Longyan, China.

Background: Cancer-related inflammation promotes gallbladder tumorigenesis and metastasis of gallbladder cancer (mGBC). The levels of circulating inflammatory-related cell and protein as well as the ratios of them may imply the severity of chronic inflammation in GBC patients, and all of them are candidate prognostic biomarkers for mGBC.

Materials And Methods: In our study, pre-treatment circulating immune cell, fibrinogen (Fib), albumin (Alb), and pre-albumin (pAlb) were detected in 220 mGBC patients, and we calculated neutrophil-to-lymphocyte ratio (NLR), derived neutrophil-to-lymphocyte ratio (dNLR), lymphocyte-to-monocyte ratio (LMR), platelet-to-lymphocyte ratio (PLR), Alb-to-Fib ratio (AFR), and Fib-to-pAlb ratio (FPR) replying on the detection. Read More

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http://dx.doi.org/10.1002/jcla.22684DOI Listing
February 2019
18 Reads

Comparison of non-invasive tests with invasive tests in the diagnosis of celiac disease.

J Clin Lab Anal 2018 Nov 21:e22722. Epub 2018 Nov 21.

Department of Pathology, Ankara Training and Research Hospital, Health Sciences University, Altındağ, Ankara, Turkey.

Background/aims: Today, invasive diagnostic tests are necessary for definite diagnosis of adult celiac disease (CD). However, in selected children patients, the need for invasive tests is ceased. In this study, we evaluated adult patients according to the ESPGHAN (European Pediatric Gastroenterology Hepatology and Nutrition Society) criteria. Read More

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http://dx.doi.org/10.1002/jcla.22722DOI Listing
November 2018
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Association of VDR-FokI and VDBP-Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case-control study in the population of China.

J Clin Lab Anal 2019 Feb 21;33(2):e22669. Epub 2018 Nov 21.

Department of Pain, the People's Hospital of Mengyin County, Mengyin, China.

Background: Cervical spondylotic myelopathy (CSM), a common degenerative disorder, is characterized by chronic progressive compression of the cervical spinal cord. The present case-control study aimed to explore the potential role of VDR-FokI and VDBP-Thr420Lys polymorphisms in the susceptibility to CSM in the Chinese population.

Methods: The study enrolled 318 CSM patients and 282 healthy individuals whose clinical data were retrospectively analyzed. Read More

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http://dx.doi.org/10.1002/jcla.22669DOI Listing
February 2019
18 Reads

Current status of treatment of type 2 diabetes mellitus in Ningbo, China.

J Clin Lab Anal 2018 Nov 21:e22717. Epub 2018 Nov 21.

Department of Endocrinology, the Affiliated Hospital of Medical School of Ningbo University, Ningbo, China.

Background: The treatment status of type 2 diabetes mellitus (T2DM) in Ningbo has not been reported in the past. To evaluate the current status of T2DM in Ningbo and provide evidence to formulate more policies, a multicenter investigation was needed.

Methods: The Ningbo Clinical Research Group of Diabetes constituted nine hospitals. Read More

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http://dx.doi.org/10.1002/jcla.22717DOI Listing
November 2018
10 Reads

Preliminary study of the relationship between promoter methylation of the ANGPTL2 gene and coronary heart disease.

J Clin Lab Anal 2018 Nov 21:e22702. Epub 2018 Nov 21.

Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China.

Background: Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Read More

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http://dx.doi.org/10.1002/jcla.22702DOI Listing
November 2018
9 Reads

A low cost and input tailing method of quality control on multiple annealing, and looping-based amplification cycles-based whole-genome amplification products.

J Clin Lab Anal 2018 Nov 21:e22697. Epub 2018 Nov 21.

Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University of Chinese PLA, Shanghai, China.

Background: Single-cell whole-genome sequencing provides novel insights into the nature of genetic heterogeneity in normal and diseased cells. However, amplification of formalin-fixed tissues with low cell numbers is still problematic and multiple annealing, and looping-based amplification cycles (MALBAC) is a commonly used whole-genome amplification (WGA) method with low cell numbers.

Methods: We developed a low-input tailing method to evaluate the MALBAC-based WGA from sub-nanogram or less quantities of input DNA. Read More

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http://dx.doi.org/10.1002/jcla.22697DOI Listing
November 2018
8 Reads