2,608 results match your criteria Journal of Clinical Laboratory Analysis[Journal]


Clinical features and treatment outcome of elderly multiple myeloma patients with impaired renal function.

J Clin Lab Anal 2019 Apr 19:e22888. Epub 2019 Apr 19.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Background: Renal impairment (RI) is a most common complication of multiple myeloma (MM), which is associated with an increased risk of early death and worse survival.

Methods: We retrospectively analyzed clinical features and outcomes of 77 MM patients over 70 years old and compared the differences between with and without RI groups.

Results: The percentage of elder MM patients with RI was 61%. Read More

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http://dx.doi.org/10.1002/jcla.22888DOI Listing

HbA1c showed a positive association with carcinoembryonic antigen (CEA) level in only diabetes, not prediabetic or normal individuals.

J Clin Lab Anal 2019 Apr 19:e22900. Epub 2019 Apr 19.

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

Background: This study was conducted to investigate the association of carcinoembryonic antigen (CEA) and glycated hemoglobin (HbA1c) in normal, prediabetic, and diabetic subjects.

Methods: A total of 2,911 participants who underwent general health checkups were enrolled and categorized into the normal, prediabetes, and diabetes groups. Demographic, anthropological, and clinical variables were investigated, and correlations with CEA were analyzed. Read More

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http://dx.doi.org/10.1002/jcla.22900DOI Listing

ESRRG promoter hypermethylation as a diagnostic and prognostic biomarker in laryngeal squamous cell carcinoma.

J Clin Lab Anal 2019 Apr 19:e22899. Epub 2019 Apr 19.

Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, China.

Background: Estrogen-related receptor gamma (ESRRG) has been identified as a tumor suppressor gene in several cancers. We aimed to evaluate ESRRG promoter methylation in laryngeal squamous cell carcinoma (LSCC) and its relative clinical value in LSCC.

Methods: Bisulfite pyrosequencing assays were performed on 91 pairs of tumor and paracancer tissues from LSCC patients in China. Read More

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http://dx.doi.org/10.1002/jcla.22899DOI Listing

Family-based Association between Allele T of rs4646536 in CYP27B1 and vitamin D deficiency.

J Clin Lab Anal 2019 Apr 16:e22898. Epub 2019 Apr 16.

College of Public Health, Zhengzhou University, Zhengzhou, China.

Background: The circulating concentration of 25(OH)D is widely applied to indicate the vitamin D status, as the directly metabolic genes of 25(OH)D, CYP2R1, and CYP27B1 are associated with the concentration of 25(OH)D. However, the contributions of allelic transmission disequilibrium of single nucleotide polymorphisms (SNPs) in these genes to vitamin D deficiency remain unclear. We aimed at investigating the family-based association between SNPs of CYP2R1 and CYP27B1 and vitamin D deficiency. Read More

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http://dx.doi.org/10.1002/jcla.22898DOI Listing

Thymidine kinase 1 concentration in pleural effusion is a diagnostic marker and survival predictor for malignant pleural effusion.

J Clin Lab Anal 2019 Apr 15:e22901. Epub 2019 Apr 15.

Department of Medical Oncology, Fuyang People's Hospital, Fuyang, China.

Objective: Thymidine kinase 1 (TK1) is a key enzyme in the pyrimidine salvage pathway. Increased TK1 concentration correlates with cell division. TK1 is an emerging biomarker in cancer diagnosis; however, its effectiveness in diagnosis and management for malignant pleural effusion (MPE) is unclear. Read More

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http://dx.doi.org/10.1002/jcla.22901DOI Listing
April 2019
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Delta neutrophil index as a prognostic marker in emergent abdominal surgery.

J Clin Lab Anal 2019 Apr 15:e22895. Epub 2019 Apr 15.

Department of Colorectal Surgery, University of Hallym College of Medicine, Hallym University Sacred Heart Hospital, Anyang, Republic of Korea.

Background: Delta neutrophil index (DNI) is the fraction of circulating immature granulocytes provided by a routine, complete blood cell analyzer. It is known to be a useful prognostic marker of sepsis. The aim of this study was to evaluate the role of DNI in the diagnosis and prognosis of patients who had undergone emergent surgery for an acute abdomen. Read More

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http://dx.doi.org/10.1002/jcla.22895DOI Listing
April 2019
3 Reads

SNPs in LncRNA genes are associated with non-small cell lung cancer in a Chinese population.

J Clin Lab Anal 2019 Apr 13:e22858. Epub 2019 Apr 13.

School of Public Health, Shanghai Jiaotong University School of Medicine, Hongqiao International Institute of Medicine, Shanghai Tongren Hospital, Shanghai, China.

Background: It has indicated that single nuclear polymorphisms (SNPs) in the regions encoding non-coding transcripts are associated with lung cancer susceptibility. In a previous microarray study, we identified 13 differentially expressed long non-coding RNAs (lncRNAs) in non-small cell lung cancer (NSCLC) and associations of SNPs in these lncRNA genes with lung cancer were unknown. We conducted a case-control study to address this issue. Read More

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http://dx.doi.org/10.1002/jcla.22858DOI Listing
April 2019
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The prognosis value of EphA3 and the androgen receptor in prostate cancer treated with radical prostatectomy.

J Clin Lab Anal 2019 Apr 8:e22871. Epub 2019 Apr 8.

Department of Clinical Laboratory, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Background: This study aimed to preliminarily assess the relationship between erythropoietin-producing hepatocellular carcinoma receptor A3 (EphA3) and androgen receptor (AR) protein expression levels and prognosis in prostate cancer (PCa) to better understand the role of EphA3 in the prognosis and progression of PCa.

Materials: We investigated the expression of EphA3 and AR in human PCa by immunohistochemistry.

Results: EphA3 and AR were both significantly upregulated in PCa, with expression mainly localized to the nucleus. Read More

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http://dx.doi.org/10.1002/jcla.22871DOI Listing
April 2019
1 Read

Correlation between of small dense low-density lipoprotein cholesterol with acute cerebral infarction and carotid atherosclerotic plaque stability.

J Clin Lab Anal 2019 Apr 6:e22891. Epub 2019 Apr 6.

Department of Clinical Laboratory, Affiliated Hospital of North China University of Science and Technology, Tangshan, China.

Background: Acute cerebral infarction (ACI) is seriously harmful to human health worldwide. However, at present, the risk of disease onset is still not accurately predicted for some people.

Methods: Five hundred and nineteen patients with ACI and 300 healthy controls were included in this study. Read More

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http://dx.doi.org/10.1002/jcla.22891DOI Listing
April 2019
1 Read

The effect of continuous blood purification on P38MAPK signaling pathway in patients with multiple organ dysfunction syndrome.

J Clin Lab Anal 2019 Apr 5:e22849. Epub 2019 Apr 5.

Emergency Department, Shanghai Ninth People's Hospital, Shanghai, China.

Objective: The aim of the study was to investigate the role of p38MAPK signaling pathway in patients with multiple organ dysfunction syndrome treated with continuous blood purification.

Methods: Blood samples were obtained to analyze the protein level of inflammatory factors (IL-1, IL-8, IL-10, and TNF-α) and phosphorylated p38MAPK by utilizing ELISA assay and Western blotting, respectively. The relative mRNA level of iNOS was detected by using RT-PCR. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.22849
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http://dx.doi.org/10.1002/jcla.22849DOI Listing
April 2019
1 Read

Blood cell parameters for screening and diagnosis of hereditary spherocytosis.

J Clin Lab Anal 2019 Apr 3:e22844. Epub 2019 Apr 3.

Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: There is currently no single index for the diagnostic screening of hereditary spherocytosis (HS). However, hematology analyzers are widely used in hospital laboratories because of their highly automated performance and quality control procedure, and detection of some blood cell parameters may be useful for the early screening of HS.

Methods: We investigated the values of blood cell parameters for the screening and differential diagnosis of HS. Read More

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http://dx.doi.org/10.1002/jcla.22844DOI Listing
April 2019
3 Reads

PCR detection for syphilis diagnosis: Status and prospects.

J Clin Lab Anal 2019 Apr 2:e22890. Epub 2019 Apr 2.

Institute of Pathogenic Biology and Key Laboratory of Special Pathogen Prevention and Control of Hunan Province, Collaborative Innovation Center for New Molecular Drug Research, University of South China, Hengyang, China.

Syphilis, a re-emerging public health problem worldwide caused by Treponema pallidum subsp pallidum (T. pallidum), usually induces systemic and chronic inflammation in hosts who do not receive timely therapy after exposing to high-risk factors such as leprous sexual contact. Before the treatment, rapid and accurate detection of syphilis is essential. Read More

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http://dx.doi.org/10.1002/jcla.22890DOI Listing
April 2019
1 Read
1.144 Impact Factor

Serum amylase activity altered by the ABO blood group system in Chinese subjects.

J Clin Lab Anal 2019 Apr 2:e22883. Epub 2019 Apr 2.

Department of Endocrinology, School of Medicine, Zhongda Hospital, Southeast University, Nanjing, China.

Objective: The underlying interactions between ABO blood group antigens and pancreatic exocrine tissue have been demonstrated, and serum amylase was synthesized by pancreatic ductal cells. Thus, we investigated the link between ABO blood type and serum amylase activity in Chinese subjects.

Methods: Our study included 343 relatively healthy Chinese individuals, and the data were retrieved from electronic medical record database. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.22883
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http://dx.doi.org/10.1002/jcla.22883DOI Listing
April 2019
4 Reads
1.144 Impact Factor

Toll-like receptors, long non-coding RNA NEAT1, and RIG-I expression are associated with HBeAg-positive chronic hepatitis B patients in the active phase.

J Clin Lab Anal 2019 Mar 29:e22886. Epub 2019 Mar 29.

Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Background: Innate immunity plays a crucial role in host-virus interactions and greatly influences viral replication including HBV infection. However, few studies have investigated the possible antiviral immune roles played by TLRs, RIG-I, and long no-coding RNA NEAT1 in chronic HBV infection (CHB) patients in clinical samples and their relationships among immune responses. In this study, we sought to investigate the mRNA expression levels of TLR1-10, RIG-I, and NEAT1 expression in HBeAg-positive CHB treatment-naïve patients with the active phase. Read More

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http://dx.doi.org/10.1002/jcla.22886DOI Listing
March 2019
3 Reads

Impact of atopy on the severity and extrapulmonary manifestations of childhood Mycoplasma pneumoniae pneumonia.

J Clin Lab Anal 2019 Mar 29:e22887. Epub 2019 Mar 29.

Department of Pediatrics, Tianjin Hospital of Integrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin, China.

Objectives: The impact of atopy on disease severity and extrapulmonary manifestations in children with Mycoplasma pneumoniae (MP) pneumonia is unknown.

Methods: Patients diagnosed with MP pneumonia between January 2016, and December 2017, were enrolled in this study. A total of 150 MP pneumonia patients were enrolled at diagnosis and divided into the atopic group (n = 48) and the nonatopic group (n = 102). Read More

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http://dx.doi.org/10.1002/jcla.22887DOI Listing
March 2019
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Association between rs2188380 and the risk of breast cancer in southwest Chinese population.

J Clin Lab Anal 2019 Mar 29:e22889. Epub 2019 Mar 29.

Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Previous studies have revealed that the single nucleotide polymorphism (SNP) rs2188380 was identified as a novel locus of gout. Interestingly, gout resulting from high serum uric acid (SUA) was also identified to be associated with the risk of breast cancer (BC). We hypothesized that maybe there was a relationship between rs2188380 and the risk of BC. Read More

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http://dx.doi.org/10.1002/jcla.22889DOI Listing
March 2019
1 Read
1.144 Impact Factor

Comparison of point-of-care and central laboratory analyzers for blood gas and lactate measurements.

J Clin Lab Anal 2019 Mar 29:e22885. Epub 2019 Mar 29.

Clinical Pathology Department, Faculty of Medicine, Cipto Mangunkusumo National Referral Hospital, University of Indonesia, Jakarta, Indonesia.

Background: Blood gas analysis and blood lactate measurement have important roles in patient management. Point-of-care (POC) testing simplifies and provides rapid blood gas and lactate measurements. This study aimed to compare pH, pCO , pO , and lactate measurements between a POC device and a benchtop blood gas analyzer typically used in a hospital central laboratory, and to evaluate the inter-device variability of the POC device. Read More

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http://dx.doi.org/10.1002/jcla.22885DOI Listing

Genetic polymorphisms of long noncoding RNA RP11-37B2.1 associate with susceptibility of tuberculosis and adverse events of antituberculosis drugs in west China.

J Clin Lab Anal 2019 Mar 28:e22880. Epub 2019 Mar 28.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Background: Little knowledge about the biological functions of RP11-37B2.1, a newly defined long noncoding RNA (lncRNA) molecule, is currently available. Previous studies have shown rs160441, located in the RP11-37B2. Read More

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http://dx.doi.org/10.1002/jcla.22880DOI Listing
March 2019
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The standardization of the report for urine cell counting-A converting factor for Sysmex UF-1000i.

J Clin Lab Anal 2019 Mar 27:e22857. Epub 2019 Mar 27.

Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese medicine, Guangzhou, China.

Background: Multicenter laboratory may apply both automated flow cytometer and microscopy for urinalysis. Automated flow cytometer such as Sysmex UF-1000i evaluates particles with native urine without centrifugation and reports as "counts per μL." Microscopic examination recommended as the reference method for urine sediment analysis reports results as "counts per HPF (or μL). Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.22857
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http://dx.doi.org/10.1002/jcla.22857DOI Listing
March 2019
10 Reads

The relationship among angiotensinogen genes polymorphisms and hs-CRP and coronary artery disease.

J Clin Lab Anal 2019 Mar 26:e22881. Epub 2019 Mar 26.

Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, Taizhou, China.

Objective: To assess the association of gene polymorphisms of angiotensinogen (AGT), the key factor in rennin-angiotensin-aldosterone system (RAAS), with high-sensitivity C-reactive protein (hs-CRP) and coronary artery disease (CAD).

Methods: The current study recruited the patients who were hospitalized and assessed by coronary angiography for suspected CAD. The patients with documented CAD served as CAD group (n = 492) while the patients without documented CAD (n = 87) served as control group. Read More

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http://dx.doi.org/10.1002/jcla.22881DOI Listing
March 2019
1 Read

Genetic diversity and drug resistance of Mycobacterium tuberculosis in Yunnan, China.

J Clin Lab Anal 2019 Mar 21:e22884. Epub 2019 Mar 21.

Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, China.

Background: China is a country with high burden of tuberculosis (TB), especially drug-resistant TB (DR-TB), which is still a serious health problem in Yunnan Province. Mycobacterium tuberculosis (MTB) is the pathogenic microorganism of TB. The epidemiological characteristics of MTB strains in local areas need to be described. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.22884
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http://dx.doi.org/10.1002/jcla.22884DOI Listing
March 2019
4 Reads

Corrigendum.

Authors:

J Clin Lab Anal 2019 Mar 24;33(3):e22829. Epub 2018 Dec 24.

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http://dx.doi.org/10.1002/jcla.22829DOI Listing

Association of PON1 gene promoter DNA methylation with the risk of Clopidogrel resistance in patients with coronary artery disease.

J Clin Lab Anal 2019 Mar 19:e22867. Epub 2019 Mar 19.

Department of Cardiology, Ningbo No. 1 Hospital, Ningbo, Zhejiang, China.

Background And Aims: The failure of therapeutic response to clopidogrel in platelet inhibition, which is called clopidogrel resistance (CR), is more likely to cause cardiovascular events. We aimed to study the contribution of promoter DNA methylation of paraoxonase 1 (PON1) to the risk of clopidogrel poor response.

Methods: Through VerifyNow P2Y12 assay, patient' platelet functions were measured. Read More

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http://dx.doi.org/10.1002/jcla.22867DOI Listing
March 2019
5 Reads

The potential value of red blood cell distribution width in patients with invasive hydatidiform mole.

J Clin Lab Anal 2019 Mar 18:e22846. Epub 2019 Mar 18.

The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Red blood cell distribution width (RDW) has attracted increasing attention in cancer. The aim of this study was to assess the changes of RDW in patients with invasive hydatidiform mole and analyze the relationship between RDW and invasive hydatidiform mole.

Methods: A retrospective analysis was performed on 102 patients diagnosed as invasive hydatidiform mole in the First Affiliated Hospital of Guangxi Medical University from January 2009 to March 2018. Read More

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http://dx.doi.org/10.1002/jcla.22846DOI Listing
March 2019
2 Reads

Up-regulation of circular RNA hsa_circ_0037909 promotes essential hypertension.

J Clin Lab Anal 2019 Mar 12:e22853. Epub 2019 Mar 12.

Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medicine School of Ningbo University, Ningbo, China.

Aims: Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may involve into the molecule regulation mechanism of EH as an important biomedical. Read More

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http://dx.doi.org/10.1002/jcla.22853DOI Listing
March 2019
1 Read

Evaluation of the BioPlex 2200 syphilis total screen (IgG/IgM) with reflex to an automated rapid plasma reagin test.

J Clin Lab Anal 2019 Mar 12:e22878. Epub 2019 Mar 12.

Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina.

Background: We evaluated the recently FDA cleared BioPlex 2200 Syphilis Total Screen and automated rapid plasma reagin (RPR) assay for the detection of total (IgG/IgM) treponemal and non-treponemal antibodies in the reverse syphilis algorithm.

Methods: Prospectively submitted samples (n = 885) were assayed by both the IgG/IgM BioPlex Syphilis Screen and the original IgG BioPlex Syphilis Screen. The IgG screen reactive samples were reflexed to traditional RPR, and IgG/IgM screen reactive samples were reflexed to the automated RPR. Read More

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http://dx.doi.org/10.1002/jcla.22878DOI Listing

Evaluation of global laboratory methods and establishing on-therapy ranges for monitoring apixaban and rivaroxaban: Experience at a single institution.

J Clin Lab Anal 2019 Mar 12:e22869. Epub 2019 Mar 12.

Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.

Background: Apixaban and rivaroxaban are approved for the prevention and treatment of deep vein thrombosis (DVT), pulmonary embolism (PE), and embolic stroke in atrial fibrillation (AF) patients. The aim of this study was to find appropriate methods of monitoring the anticoagulant effects of are direct oral anticoagulants (DOACs) and establish on-therapy ranges using conventional tests.

Methods: A total of 184 samples were collected from 91 patients receiving DOACs. Read More

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http://dx.doi.org/10.1002/jcla.22869DOI Listing
March 2019
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Prevalence of Hepatitis C virus genotypes in nine selected European countries: A systematic review.

J Clin Lab Anal 2019 Mar 7:e22876. Epub 2019 Mar 7.

Scientific Director, IRCCS Fondazione Pascale, Naples, Italy.

Background: Hepatitis C virus (HCV) infection is a global health problem especially for its increasing level of mortality. Detailed knowledge of HCV genotypes prevalence has clinical relevance since the efficacy of therapies is impacted by genotypes and subtypes distribution. Moreover, HCV exhibits a great genetic variability regionally. Read More

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http://dx.doi.org/10.1002/jcla.22876DOI Listing
March 2019
1 Read

Pooled analysis of nuclear acid sequence-based amplification for rapid diagnosis of Mycoplasma pneumoniae infection.

J Clin Lab Anal 2019 Mar 6:e22879. Epub 2019 Mar 6.

Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, China.

Background: Mycoplasma pneumoniae (M pneumoniae) is a common human etiology of respiratory infections. Nuclear acid sequence-based amplification (NASBA) shows good value for the detection of M pneumoniae that surpasses PCR. However, the optimal detection technology still remains to be identified. Read More

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http://dx.doi.org/10.1002/jcla.22879DOI Listing

Establishing and validating of an laboratory information system-based auto-verification system for biochemical test results in cancer patients.

J Clin Lab Anal 2019 Mar 6:e22877. Epub 2019 Mar 6.

Department of Clinical Laboratory, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: To establish and validate an laboratory information system (LIS)-based auto-verification (AV) system by using large amounts of biochemical test results in cancer patients.

Methods: An algorithm of the AV process was designed for pre-analysis, analysis, and post-analysis. The limit range check was adjusted three times, while the delta check criteria were first replaced by the same patients' historical extremum results. Read More

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http://dx.doi.org/10.1002/jcla.22877DOI Listing
March 2019
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IDO and intra-tumoral neutrophils were independent prognostic factors for overall survival for hepatocellular carcinoma.

J Clin Lab Anal 2019 Mar 6:e22872. Epub 2019 Mar 6.

Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, China.

Background: Both Indole-amine-2,3-dioxygenase (IDO) and neutrophils were proved to have pro-tumor effect in some kinds of solid tumors by immune suppression. However, little is known about their effect on hepatocellular carcinoma (HCC) and the relationship between these two immune-suppressive factors. The aim of this study was to evaluate the prognostic significance of IDO and intra-tumoral neutrophils and their correlations in HCC. Read More

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http://doi.wiley.com/10.1002/jcla.22872
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http://dx.doi.org/10.1002/jcla.22872DOI Listing
March 2019
4 Reads

The association of five preoperative serum tumor markers and pathological features in patients with breast cancer.

J Clin Lab Anal 2019 Mar 6:e22875. Epub 2019 Mar 6.

Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen, China.

Background: The utility of frequently used serum tumor markers in breast cancer remains controversial. The study aimed to investigate the role of preoperative carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), cancer antigen 153 (CA153), cancer antigen 724 (CA724), and ferritin (FER) in the management of breast cancer and their relationships with pathological features.

Methods: A total of 804 patients with breast mass who underwent breast surgery and 305 healthy volunteers were enrolled. Read More

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http://dx.doi.org/10.1002/jcla.22875DOI Listing

Aberrant expression of serum circANRIL and hsa_circ_0123996 in children with Kawasaki disease.

J Clin Lab Anal 2019 Mar 6:e22874. Epub 2019 Mar 6.

Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, China.

Background: Kawasaki disease is a childhood systemic vasculitis that causes coronary artery abnormalities. The etiology remains unknown and there are no specific diagnostic tests. Circular non-coding RNAs are a special class of endogenous RNAs that display some characteristics of an ideal biomarker. Read More

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http://dx.doi.org/10.1002/jcla.22874DOI Listing
March 2019
10 Reads

The clinical significance of HOXA9 promoter hypermethylation in head and neck squamous cell carcinoma.

J Clin Lab Anal 2019 Mar 6:e22873. Epub 2019 Mar 6.

Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, China.

Background: The purpose of the current study was to assess the association between HOXA9 (homeobox A9) promoter methylation and head and neck squamous cell carcinoma (HNSCC) and its diagnostic value.

Methods: Quantitative methylation-specific PCR (qMSP) was applied to measure HOXA9 promoter methylation levels in 145 paired HNSCC and corresponding normal tissue samples. Data from the Cancer Genome Atlas (TCGA) database (n = 578; 528 HNSCC and 50 normal) were also analyzed. Read More

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http://dx.doi.org/10.1002/jcla.22873DOI Listing

Evaluation of Nova StatStrip and FreeStyle Precision Pro blood ketone tests using 3-hydroxybutyrate doped samples.

J Clin Lab Anal 2019 Feb 27:e22851. Epub 2019 Feb 27.

Centre for Inherited Metabolic Diseases, Karolinska University Hospital, Stockholm, Sweden.

Background: The most clinically useful blood ketone in the diagnosis, management, and recovery of diabetes ketoacidosis in both adults and children is 3-hydroxybutyrate. In the absence of laboratory routine methods, several point-of-care methods are in use, but very few clinical evaluations are published.

Methods: This study evaluates linearity and reproducibility of two handheld point-of-care meters for blood 3-hydroxybutyrate measurement for hospital use, Nova StatStrip, and FreeStyle Precision Pro. Read More

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http://dx.doi.org/10.1002/jcla.22851DOI Listing
February 2019

Next-generation sequencing improves molecular epidemiological characterization of thalassemia in Chenzhou Region, P.R. China.

J Clin Lab Anal 2019 Feb 27:e22845. Epub 2019 Feb 27.

Center of Prenatal Diagnosis, Chenzhou No. 1 People's Hospital, Chenzhou, China.

Objectives: Thalassemia is a highly prevalent monogenic inherited disease in southern China. It is important to collect epidemiological data comprehensively for proper prevention and treatment.

Methods: In this study, blood samples collected from 15 807 residents of Chenzhou were primarily screened by hematological tests. Read More

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http://doi.wiley.com/10.1002/jcla.22845
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http://dx.doi.org/10.1002/jcla.22845DOI Listing
February 2019
9 Reads
1.144 Impact Factor

Circulating lncRNA ITSN1-2 is upregulated, and its high expression correlates with increased disease severity, elevated inflammation, and poor survival in sepsis patients.

J Clin Lab Anal 2019 Feb 25:e22836. Epub 2019 Feb 25.

Department of Emergency Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: This study aimed to assess the correlation of long noncoding (lnc) RNA intersectin (ITSN) 1-2 expression with disease risk, severity, inflammation, and survival in sepsis patients.

Methods: Three hundred and nine intensive care unit (ICU)-treated sepsis patients and 300 healthy controls were consecutively recruited in this study. Blood samples were collected from all sepsis patients within 24 hours after admitted to ICU and from healthy controls at the time of health screening, and the expression of lncRNA ITSN1-2 in plasma was detected by quantitative polymerase chain reaction. Read More

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http://dx.doi.org/10.1002/jcla.22836DOI Listing
February 2019

Semiquantitative, fully automated urine test strip analysis.

J Clin Lab Anal 2019 Feb 25:e22870. Epub 2019 Feb 25.

Department of Laboratory Medicine, Ghent University Hospital, Gent, Belgium.

Objectives: Urinalysis is one of the most frequently ordered diagnostic laboratory tests. In order to reduce workload and costs, rapid screening tests such as urine test strip analyses are applied. The aim of this study was to evaluate the analytical performance of the UC-3500 as well as the diagnostic performance in comparison with reference methods. Read More

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http://dx.doi.org/10.1002/jcla.22870DOI Listing
February 2019
2 Reads

Detection of Ureaplasma spp. serovars in genital tract of infertile males.

J Clin Lab Anal 2019 Feb 25:e22865. Epub 2019 Feb 25.

Department of Clinical Laboratory, Sir Run Run Shaw hospital, School of medicine, Zhejiang University, Hangzhou, China.

Background: The colonization of Ureaplasma species in genital tract is related with male infertility. However, it has been postulated based upon limited study that virulence is related to serotype specificity. The aim of this study was to determine the distribution of Ureaplasma serovars in genital tract of infertile males and analyze their role in male infertility. Read More

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http://dx.doi.org/10.1002/jcla.22865DOI Listing
February 2019
2 Reads

Study on the diagnosis of gout with xanthine and hypoxanthine.

J Clin Lab Anal 2019 Feb 25:e22868. Epub 2019 Feb 25.

West China Hospital, Sichuan University, Chengdu, China.

Background: Hyperuricemia is the only biochemical index in the classification of acute gouty arthritis in American Rheumatism Association 1977 and the main basis of clinical diagnosis for most doctors. However, nearly half of the time gout occurs without hyperuricemia, especially in an acute attack,which leads to an urgent need to find a new substitute diadynamic criteria of gout. Xanthine and hypoxanthine, as precursors of uric acid, have been reported to be high in gout patients with hyperuricemia and presumed to be gout biomarkers. Read More

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http://doi.wiley.com/10.1002/jcla.22868
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http://dx.doi.org/10.1002/jcla.22868DOI Listing
February 2019
9 Reads

Sample-to-cutoff ratios using Architect HIV Ag/Ab Combo: The influence with the results of supplemental tests and optimal cutoff value to predict HIV infection.

J Clin Lab Anal 2019 Feb 25:e22866. Epub 2019 Feb 25.

Xi'an NO.3 Hospital, Xi'an, China.

Background: The Architect HIV Ag/Ab Combo has excellent performance for HIV screening; however, the false-positive rate (FPR) was high in low HIV prevalence setting.

Objectives: The purpose of this study was to analyze the influence of sample-to-cutoff (s/co) ratios by Architect HIV Ag/Ab Combo with the results of confirmatory test and explore the potential utility of s/co to predict HIV infection.

Methods: A retrospective review on Architect HIV Ag/Ab Combo reactive results was performed at a teaching hospital in Xi'an. Read More

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http://dx.doi.org/10.1002/jcla.22866DOI Listing
February 2019
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Increased serum adiponectin predicts improved coronary flow and clinical outcomes in patients with ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention.

J Clin Lab Anal 2019 Feb 19:e22864. Epub 2019 Feb 19.

Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with ST-segment elevation myocardial infarction (STEMI) is unclear.

Methods: A total of 144 patients with STEMI treated by PPCI were enrolled and divided into two groups based on the mean serum APN level on admission. Read More

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http://dx.doi.org/10.1002/jcla.22864DOI Listing
February 2019

Upregulation of miR-146a-5p is associated with increased proliferation and migration of vascular smooth muscle cells in aortic dissection.

J Clin Lab Anal 2019 Feb 19:e22843. Epub 2019 Feb 19.

Guangdong Cardiovascular Institute, Guangdong Provincial People' Hospital, Guangdong Academy of Medical Sciences, Guangdong, Guangzhou, China.

Background: This study aimed to investigate whether miR-146a-5p was involved in the pathogenesis of thoracic aortic dissection (AD) via regulating the biological function of vascular smooth muscle cells (VSMCs).

Methods: Circulating miR-146a-5p level was measured by quantitative polymerase chain reaction (qPCR) in AD patients and healthy controls. Human dissected aortic samples were obtained from patients with thoracic AD Stanford type A undergoing surgical repair, and normal control samples were from organ donors who died from nonvascular diseases. Read More

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http://dx.doi.org/10.1002/jcla.22843DOI Listing
February 2019
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Distribution of serum neuron-specific enolase and the establishment of a population reference interval in healthy adults.

J Clin Lab Anal 2019 Feb 19:e22863. Epub 2019 Feb 19.

Department of Laboratory Medicine, The Second People's Hospital of Lianyungang, Lianyungang, China.

Background: Neuron-specific enolase (NSE) is an important tumor marker in the serum of patients with lung cancer. Elevated serum NSE levels are also associated with many other diseases. However, there is no unified population reference interval for serum NSE. Read More

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February 2019
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Determination of hemolysis index thresholds for biochemical tests on Siemens Advia 2400 chemistry analyzer.

J Clin Lab Anal 2019 Feb 19:e22856. Epub 2019 Feb 19.

Department of clinical laboratory, Characteristic Medical center of Chinese People's Armed Police Force, Pingjin Hospital, Tianjin, China.

Background: In vitro hemolysis is still the most common source of pre-analytical nonconformities. This study aimed to investigate the hemolytic effects on commonly used biochemical tests as well as to determine the hemolysis index (HI) thresholds on Siemens Advia 2400 chemistry analyzer.

Methods: Peripheral blood samples were collected from forty healthy volunteers. Read More

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http://dx.doi.org/10.1002/jcla.22856DOI Listing
February 2019
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The clinical value of hematological markers in rheumatoid arthritis patients treated with tocilizumab.

J Clin Lab Anal 2019 Feb 19:e22862. Epub 2019 Feb 19.

Department of Preventive Medicine, Medical School of Ningbo University, Ningbo, China.

Background: Emerging evidence indicates that some hematological markers have critical value in evaluating treatment response. This study was performed to determine the clinical value of hemoglobin (Hb), platelet (Plt), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in rheumatoid arthritis (RA) patients treated with tocilizumab (TCZ).

Methods: Fifty-two RA patients receiving TCZ were recruited and followed for 6 months. Read More

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http://dx.doi.org/10.1002/jcla.22862DOI Listing
February 2019
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Intra- and inter-laboratory agreement of the FAM19A4/mir124-2 methylation test: Results from an international study.

J Clin Lab Anal 2019 Feb 13:e22854. Epub 2019 Feb 13.

Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, Amsterdam, the Netherlands.

Background: HPV-based cervical screening detects women at an increased risk of cervical cancer and precancer. To differentiate among HPV-positive women those with (pre)cancer, triage testing is necessary. The detection of cancer-associated host-cell DNA methylation (FAM19A4 and hsa-mir124-2) in cervical samples has shown valuable as triage test. Read More

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http://dx.doi.org/10.1002/jcla.22854DOI Listing
February 2019

Establishment of a bead-based duplex assay for the simultaneous quantitative detection of Neuropilin-1 and Neuropilin-2 using xMAP technology and its clinical application.

J Clin Lab Anal 2019 Feb 13:e22850. Epub 2019 Feb 13.

Cancer Research Center, Medical College, Xiamen University, Xiamen, China.

Background: Neuropilins (Nrps) are a new type of broad-spectrum tumor marker. Currently, a method for accurate simultaneous quantification of Nrps is not available. We aimed to develop a bead-based and duplexed flow cytometric assay that could be used for accurate and simultaneous quantification of Nrp1 and Nrp2 for scientific research or clinical diagnosis. Read More

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http://dx.doi.org/10.1002/jcla.22850DOI Listing
February 2019

Predictive value of soluble fms-like tyrosine kinase-1 against placental growth factor for preeclampsia in a Chinese pregnant women population.

J Clin Lab Anal 2019 Feb 13:e22861. Epub 2019 Feb 13.

Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu, China.

Objective: The purpose of the present study was to explore the predictive effects of soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) for preeclampsia.

Methods: A total of 1580 singleton pregnant women aged 18-45 years were included in this study. Serum samples were collected and stored frozen during their regular obstetric examinations. Read More

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http://doi.wiley.com/10.1002/jcla.22861
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http://dx.doi.org/10.1002/jcla.22861DOI Listing
February 2019
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Interleukin-21 receptor gene polymorphism is associated with hepatitis B virus-related hepatocellular carcinoma in Chinese patients.

J Clin Lab Anal 2019 Feb 13:e22860. Epub 2019 Feb 13.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chendu, Sichuan, China.

Background: We investigated the relationship between hepatitis B virus (HBV)-related pathogenesis and single nucleotide polymorphisms (SNPs) in interleukin-21 (IL-21)-JAK-STAT signaling pathway genes.

Methods: We used the high-resolution melting (HRM) method to genotype five SNPs (IL-21 rs2221903, IL-21 rs4833837, IL-21 receptor (IL-21R) rs2285452, JAK3 rs3008, and STAT3 rs1053023) in 546 HBV-infected patients and 353 healthy Chinese subjects. The HBV-infected patients were further divided into subgroups based on the HBV-related pathologies: chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC), and HBV-related hepatocellular carcinoma (HCC). Read More

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http://dx.doi.org/10.1002/jcla.22860DOI Listing
February 2019
1 Read