9,747 results match your criteria Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences[Journal]


Untargeted LC/MS-based metabolic phenotyping (metabonomics/metabolomics): The state of the art.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 8;1117:136-147. Epub 2019 Apr 8.

Division of Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College, Exhibition Road, South Kensington, London SW7 2AZ, UK. Electronic address:

Liquid chromatography (LC) hyphenated to mass spectrometry is currently the most widely used means of determining metabolic phenotypes via both untargeted and targeted analysis. At present a range of analytical separations, including reversed-phase, hydrophilic interaction and ion-pair LC are employed to maximise metabolome coverage with ultra (high) performance liquid chromatography (UHPLC) increasingly displacing conventional high performance liquid chromatography because of the need for short analysis times and high peak capacity in such applications. However, it is widely recognized that these methodologies do not entirely solve the problems facing researchers trying to perform comprehensive metabolic phenotyping and in addition to these "routine" approaches there are continuing investigations of alternative separation methods including 2-dimensional/multi column approaches. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.009DOI Listing

Liquid chromatography-mass spectrometry-based determination of ergocristine, ergocryptine, ergotamine, ergovaline, hypoglycin A, lolitrem B, methylene cyclopropyl acetic acid carnitine, N-acetylloline, N-formylloline, paxilline, and peramine in equine hair.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 5;1117:127-135. Epub 2019 Apr 5.

Institute of Forensic Medicine, Jena University Hospital, Jena, Germany. Electronic address:

Ingestion of hypoglycin A (HGA) in maple seeds or alkaloids produced by symbiotic fungi in pasture grasses is thought to be associated with various syndromes in grazing animals. This article describes analytical methods for monitoring long-term exposure to HGA, its metabolite MCPA-carnitine, as well as ergocristine, ergocryptine, ergotamine, ergovaline, lolitrem B, N-acetylloline, N-formylloline, peramine, and paxilline in equine hair. After extraction of hair samples separation was achieved using two ultra high performance liquid chromatographic systems (HILIC or RP-C18, ammonium formate:acetonitrile). Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.016DOI Listing

Minicircle DNA purification: Performance of chromatographic monoliths bearing lysine and cadaverine ligands.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 14;1118-1119:7-16. Epub 2019 Apr 14.

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal. Electronic address:

Minicircle DNA (mcDNA) technology is in the vanguard of vectors designed for gene therapy, since the absence of prokaryotic sequences confers to mcDNA higher biosafety in comparison to other DNA vectors. However, the presence of other isoforms and non-recombined parental molecules hampers the isolation of supercoiled (sc) mcDNA with the chromatographic methods already established for plasmid purification. In this work, two monolithic supports were modified with lysine and its decarboxylated derivative, cadaverine, to explore their performance in the sc mcDNA purification. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183170
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http://dx.doi.org/10.1016/j.jchromb.2019.04.024DOI Listing
April 2019
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Determination of total or free cefazolin and metronidazole in human plasma or interstitial fluid by HPLC-UV for pharmacokinetic studies in man.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 12;1118-1119:51-54. Epub 2019 Apr 12.

University of Leipzig, Integrated Research and Treatment Center (IFB) Adiposity Diseases, Leipzig, Germany; Dept. of Anaesthesiology and Intensive Care Medicine, University Hospital Leipzig, Leipzig, Germany.

Cefazolin (CFZ) plus metronidazole (MTZ) is commonly used for perioperative antibiotic prophylaxis. An HPLC-UV method is described for the simultaneous determination of total or free cefazolin and metronidazole in human plasma or in microdialysate of subcutaneous tissue. Separation was performed isocratically using a reversed phase column and phosphate buffer/acetonitrile as mobile phase. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.025DOI Listing

An FPSE-HPLC-PDA method for rapid determination of solar UV filters in human whole blood, plasma and urine.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 16;1118-1119:40-50. Epub 2019 Apr 16.

International Forensic Research Institute, Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA. Electronic address:

This paper reports a novel fabric phase sorptive extraction-high performance liquid chromatography-photodiode array detection (FPSE-HPLC-PDA) method for the simultaneous extraction and analysis of six benzophenone derivative UV filters including benzophenone (BZ); 5-benzoyl-4-hydroxy-methoxybenzenesulfonic acid (BP-4); bis(4-hydroxyphenyl)methanone (4-DHB); bis(2,4-dihydroxyphenyl)methanone (BP-2); (2,4-dihydroxybenzophenone) (BP-1) and 2,2'-dihydroxy-4-methoxybenzophenone (DHMB) in human whole blood, plasma and urine samples. Chromatographic separation method was conducted using a Spherisorb ODS 1 (C) column in isocratic elution mode with a run time <25 min. The FPSE-HPLC-PDA method was validated in the range from 0. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.028DOI Listing
April 2019
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Analysis of PK11195 concentrations in rodent whole blood and tissue samples by rapid and reproducible chromatographic method to support target-occupancy PET studies.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 12;1118-1119:33-39. Epub 2019 Apr 12.

University/BHF Centre for Cardiovascular Science, University of Edinburgh, UK; Edinburgh Preclinical Imaging (EPI), University of Edinburgh, UK.

In Positron Emission Tomography (PET) research, it is important to assess not only pharmacokinetics of a radiotracer in vivo, but also of the drugs used in blocking/displacement PET studies. Typically, pharmacokinetic/pharmacodynamic (PK/PD) analyses of drugs used in rodent PET studies are based on population average pharmacokinetic profiles of the drugs due to limited blood volume withdrawal while simultaneously maintaining physiological homeostasis. This likely results in bias of PET data quantification, including unknown bias of target occupancy (TO) measurements. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.026DOI Listing

Untargeted gas chromatography-mass spectrometry-based metabolomics analysis of kidney and liver tissue from the Lewis Polycystic Kidney rat.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 12;1118-1119:25-32. Epub 2019 Apr 12.

Separation Science and Metabolomics Laboratory, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia; Metabolomics Australia, Western Australia Node, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.

Polycystic kidney disease (PKD) encompasses a spectrum of inherited disorders that lead to end-stage renal disease (ESRD). There is no cure for PKD and current treatment options are limited to renal replacement therapy and transplantation. A better understanding of the pathobiology of PKD is needed for the development of new, less invasive treatments. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.021DOI Listing

Bract as a novel extraction phase in thin-film SPME combined with 96-well plate system for the high-throughput determination of estrogens in human urine by liquid chromatography coupled to fluorescence detection.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 16;1118-1119:17-24. Epub 2019 Apr 16.

Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis 88040900, SC, Brazil. Electronic address:

In this study, an environmentally friendly and high-throughput method was developed for the determination of estrone (E1), 17β-estradiol (E2), 17α-ethinylestradiol (EE2) and estriol (E3) in human urine by liquid chromatography-fluorescence detector (HPLC-FLD). A biosorbent (bract) was proposed as extraction phase for Thin-Film SPME combined with 96-well system. The characterization of the biosorbent was performed by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.037DOI Listing

Mechanistic investigation of phosphoprotein enrichment by fly ash-based chromatography.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 16;1118-1119:1-6. Epub 2019 Apr 16.

Faculty of Medical Technology, Prince of Songkla University, Songkhla, Thailand. Electronic address:

In this work, the mechanistic details contributing to the binding of phosphoproteins on fly ash (FA) has been investigated. The effects of factors influencing adsorption of phosphoprotein, i.e. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.035DOI Listing
April 2019
1 Read

High performance liquid chromatography (HPLC) based direct and simultaneous estimation of excipients in biopharmaceutical products.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 12;1117:118-126. Epub 2019 Apr 12.

Department of Chemical Engineering, Indian Institute of Technology, 110016 Hauz Khas, India. Electronic address:

Excipients are critical components of a biotherapeutic product as they have a direct impact on the product's stability and hence on its safety and efficacy. A typical biotherapeutic drug product has a variety of excipients and these need to be maintained within very tight specifications in order to ensure consistency in product quality. Hence, there is always a need for estimation of excipient concentration in the drug product. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.022DOI Listing

Hydrophobic-interaction chromatography for purification of influenza A and B virus.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 1;1117:103-117. Epub 2019 Apr 1.

Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, Germany; Chair of Bioprocess Engineering, Otto-von-Guericke University, 39106 Magdeburg, Germany.

Options for hydrophobic-interaction chromatography (HIC) for purification of cell culture-derived influenza A and B virus have been assessed using a 96-well plate format using a semi-high-throughput approach. Follow-up experiments at preparative scale were used to characterize dynamic binding capacity, viral hemagglutinin protein (HA protein) recovery, and the influence of influenza virus (IV) strains on yield and contamination levels. Virus recoveries of up to 96% with a residual DNA level of about 1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232193016
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http://dx.doi.org/10.1016/j.jchromb.2019.03.037DOI Listing
April 2019
1 Read

Simultaneous determination of pesticides, mycotoxins, and metabolites as well as other contaminants in cereals by LC-LC-MS/MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 6;1117:86-102. Epub 2019 Apr 6.

Food Chemistry, Technical University Dresden, Bergstraße 66, D-01069 Dresden, Germany.

A 2D LC-MS/MS method for the simultaneous determination of 350 pesticides, 16 mycotoxins as well as the growth regulators Chlormequat and Mepiquat was developed. The method is applicable to cereals and products thereof. Attention should be paid to the simultaneous analysis of the cereal-relevant mycotoxins aflatoxin B1, B2, G1 and G2, ochratoxin A, deoxynivalenol and zearalenone. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.013DOI Listing

Development and validation of an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid and creatinine in porcine and broiler chicken plasma.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 8;1117:77-85. Epub 2019 Apr 8.

Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, Ghent University, Merelbeke, Belgium. Electronic address:

In order to study the renal function, in terms of glomerular filtration and effective renal plasma flow, in broiler chickens and pigs, an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid (PAH) and exogenously administered creatinine in plasma was developed and validated. Sample preparation consisted of a deproteinization step using methanol for porcine plasma and an Ostro™ Protein Precipitation & Phospholipid Removal Plate was used for broiler chicken plasma. Chromatographic separation was achieved on a Hypersil Gold aQ column using 0. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.017DOI Listing

An LC-MS/MS method to profile urinary mercapturic acids, metabolites of electrophilic intermediates of occupational and environmental toxicants.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 6;1117:66-76. Epub 2019 Apr 6.

EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address:

Introduction: Mercapturic acids are urinary metabolites of occupational and environmental toxicants. The aim of this work was to develop and validate an analytical assay for the determination of several urinary mercapturic acids to be used as biomarkers of exposure.

Method: An isotope dilution tandem mass spectrometric method, coupled with reversed-phase liquid chromatography, was developed for the analysis of mercapturic acids derived from several compounds, including those of benzene, toluene, 1,3-butadiene, styrene, acrylonitrile, 4-chloronitrobenzene, acrylamide, acrolein, propylene oxide, N,N-dimethylformamide, crotonaldehyde, ethylene oxide, and methylating and ethylating agents. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.015DOI Listing

UHPLC assisted simultaneous separation of apigenin and prednisolone and its application in the pharmacokinetics of apigenin.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 3;1117:58-65. Epub 2019 Apr 3.

Kayyali Chair for Pharmaceutical Industries, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, P.O. Box: 2457, Saudi Arabia. Electronic address:

A new ultra-high performance liquid chromatography-mass spectrometry-mass spectrometry (UHPLC-MS/MS) system has been formulated for the resolution of closely related drugs apigenin (API, a bioflavinoid) and prednisolone (PRD) from their mixture. This developed method comprised of a "BEH™ C column (50 mm × 2.1 mm, 1. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.006DOI Listing

Multiplatform metabolomics provides insight into the molecular basis of chronic kidney disease.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 3;1117:49-57. Epub 2019 Apr 3.

Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

Changes in metabolites composition can reflect currently present pathological processes in a living organism and constitute a basis for diagnosis and treatment improvements. Thus, the multiplatform metabolomics approach was applied for the investigation of molecular mechanisms of chronic kidney disease (CKD) progression. The high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS) and gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ/MS) serum metabolic fingerprinting followed by uni- and multivariate statistical analysis was carried out to determine metabolic pattern differentiating CKD patients and healthy controls. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183116
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http://dx.doi.org/10.1016/j.jchromb.2019.04.003DOI Listing
April 2019
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Quantitation of vitamin K1 in serum using online SPE-LC-MS/MS and the challenges of working with vitamin K.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 7;1117:41-48. Epub 2019 Apr 7.

Department of Clinical Biochemistry and Immunology, Lillebaelt Hospital, Vejle, Denmark.

Vitamin K1 (phylloquinone) is one of the vitamin Ks. Several studies have previously investigated the role of vitamin K1 status in respect to disease, but without consistent results. Since vitamin K deficiency has been associated with different disease states it is important to develop a biochemical analysis with sufficient sensitivity and a low limit of quantitation (LOQ). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183176
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http://dx.doi.org/10.1016/j.jchromb.2019.04.010DOI Listing
April 2019
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Simultaneous quantitation of 9 anabolic and natural steroidal hormones in equine urine by UHPLC-MS/MS triple quadrupole.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 2;1117:36-40. Epub 2019 Apr 2.

School of Pharmacy, University of Camerino, Camerino, Italy.

A new fast and easy analytical procedure for the simultaneous detection and quantification of 9 anabolic steroids (deslorelin, dexamethasone sodium phosphate, prednisolone, methylprednisolone, stanozolol, boldenone, nandrolone, dexamethasone isonicotinate and altrenogest) in horse urine for doping control have been developed by using the ultra-high-performance liquid chromatography coupled with tandem mass spectrometry technique (UHPLC-MS/MS). A total amount of 400 μl of sample was evaporated, restored and injected in the UHPLC-MS/MS. The proposed method was fully validated showing a recovery higher than 89. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.002DOI Listing
April 2019
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Comprehensive metabolism study of polydatin in rat plasma and urine using ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 4;1117:22-35. Epub 2019 Apr 4.

Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address:

Polydatin is one of the main bioactive constituents isolated from Polygonum cuspidatum Sieb. et Zucc., which possesses various pharmacological activities. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232193022
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http://dx.doi.org/10.1016/j.jchromb.2019.04.005DOI Listing
April 2019
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A pilot case-control study of urine metabolomics in preterm neonates with necrotizing enterocolitis.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 8;1117:10-21. Epub 2019 Apr 8.

1(st) Department of Neonatology, School of Medicine, Aristotle University of Thessaloniki, Greece; Biomic_AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, B1.4, Thessaloniki, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, 57001, Greece. Electronic address:

Necrotizing enterocolitis (NEC) is a leading cause of gastrointestinal morbidity and mortality in preterm neonates. The aim of this pilot study was to explore using metabolomics alternations in the urine metabolites related to NEC that could possibly serve as diagnostic biomarkers of the disease. Urine samples were prospectively collected at the day of initial evaluation for NEC from 15 diseased preterm neonates (five Bell's stage I and ten stage II/III) and an equal number of matched controls. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232193008
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http://dx.doi.org/10.1016/j.jchromb.2019.04.019DOI Listing
April 2019
1 Read

Measurement of serum steroid profiles by HPLC-tandem mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 3;1117:1-9. Epub 2019 Apr 3.

Department of Laboratory Medicine, Seoul National University Bundang Hospital, Gyeonggi-do, Republic of Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address:

Accurate investigations of adrenal hormone levels play a vital role in pediatric endocrinology for the detection of steroid-related disorders. In this study, we developed and validated a simultaneous assay for eight serum steroids, i.e. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.001DOI Listing
April 2019
2 Reads
2.729 Impact Factor

GC-MS analysis of D-pinitol in carob: Syrup and fruit (flesh and seed).

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 3;1116:60-64. Epub 2019 Apr 3.

Department of Chemistry, University of Cyprus, P.O. Box 20537, 1678 Nicosia, Cyprus. Electronic address:

D-pinitol (3-O-methyl-D-chiro-inositol) is a well-known bioactive compound with anti-diabetic and anti-oxidant biological functions. A gas chromatography-mass spectrometry (GC-MS) method was developed for its quantitation in carob syrup, flesh and seed samples originated from Cyprus. The analysis was performed after derivatization of carbohydrates and polyols into trimethylsilyl ether derivatives. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232193036
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http://dx.doi.org/10.1016/j.jchromb.2019.04.008DOI Listing
May 2019
2 Reads

A method to directly analyze free-drug-related species in antibody-drug conjugates without sample preparation.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 5;1116:51-59. Epub 2019 Apr 5.

Department of Analytical Sciences, MedImmune, One Medimmune Way, Gaithersburg, MD 20878, USA. Electronic address:

Residual free-drug-related species that are present in antibody-drug conjugates (ADC) are a potential safety risk to patients and are therefore categorized as a critical quality attribute that must be strictly monitored and controlled. Among the many analytical methods developed for free-drug analysis, reversed-phase liquid chromatography (RP-LC) is the most common approach. Conventional RP-LC methods for free-drug analysis, however, involve labor-intensive sample preparation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183177
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http://dx.doi.org/10.1016/j.jchromb.2019.04.012DOI Listing
May 2019
12 Reads

Development and validation of a sensitive LC-MS/MS method for the determination of 6-hydroxykynurenic acid in rat plasma and its application to pharmacokinetics study.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 27;1116:44-50. Epub 2019 Mar 27.

Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address:

A sensitive and specific bioanalytical method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification of 6-hydroxykynurenic acid (6-HKA) in a small quantity of rat plasma has been developed and comprehensively validated. Tolbutamide (Tol) was used as the internal standard (IS). An aliquot of 50 μL rat plasma sample was deproteinized by 150 μL methanol, and then 100 μL of its supernatant was mixed with 100 μL water after centrifugation. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.033DOI Listing
May 2019
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Determination of daptomycin in human plasma and breast milk by UPLC/MS-MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 27;1116:38-43. Epub 2019 Mar 27.

Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy. Electronic address:

During the lactation, the choice of a proper antibiotic is crucial since the drug can cross into breast milk causing toxicity to the infant. Therefore, an extraction protocol and LC/MS-MS method for the determination of daptomycin in human milk and plasma were developed, validated and applied to a case of a breastfeeding mother affected by a purulent acute soft skin infection treated with daptomycin. Because of daptomycin high protein binding and its high molecular weight, the optimisation of the extraction protocol and analytical conditions were deeply investigated, and several parameters were taken into account: in particular the type of extraction, internal standard, the type of organic modifier, pH of the aqueous solution, and gradient. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.036DOI Listing
May 2019
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Development and validation of an extraction method for the analysis of perfluoroalkyl substances (PFASs) in environmental and biotic matrices.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 28;1116:30-37. Epub 2019 Mar 28.

Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address:

Although long chained PFASs have been phased-out in several countries, their persistence in the environment and bioaccumulative potential cause the environmental and biotic concentrations to remain high, highlighting the need to further monitor these pollutants. Currently several methods are used for the quantification of perfluoroalkyl substances (PFASs) in biological matrices including different ways to correct for recovery losses, each with its specific pros and contras. With this paper we aim to re-evaluate current methodologies and to create an updated new analytical guideline that is applicable for both abiotic and biotic matrices. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.034DOI Listing
May 2019
3 Reads

Miniaturized imprinted solid phase extraction to the selective analysis of Coenzyme Q10 in urine.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 23;1116:24-29. Epub 2019 Mar 23.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Tecnología Farmacéutica, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Argentina. Electronic address:

Coenzyme Q10 (CoQ10) is an important cofactor in the mitochondrial respiratory chain and a potent endogenous antioxidant. CoQ10 deficiency is currently associated with numerous diseases like mitochondrial and neurodegenerative pathologies, in which the earliest diagnosis and treatment with CoQ10 supplementation becomes paramount for patient's treatment. Consequently, the determination of CoQ10 levels in different biological matrices positions as a fundamental tool. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.029DOI Listing
May 2019
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Quantification of residual AEBSF-related impurities by reversed-phase liquid chromatography.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 20;1116:19-23. Epub 2019 Mar 20.

Vaccine Production Program, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Gaithersburg, MD, USA. Electronic address:

During research of a broadly neutralizing antibody (bNAb) for HIV-1 infection, site-specific clipping was observed during cell culture incubation. Protease inhibitor, 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF), was supplemented to the cell culture feeding to mitigate clipping as one of the control strategies. It led to the need and development of a new assay to monitor the free AEBSF-related impurities during the purification process. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.022DOI Listing
May 2019
5 Reads

In-vitro metabolic profiling study of potential topoisomerase inhibitors 'pyrazolines' in RLMs by mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 23;1114-1115:125-133. Epub 2019 Mar 23.

Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address:

Taking into consideration of the cytotoxicity and topo-IIα inhibitory activity of pyrazoline derivatives (1-3) against HCT15 cells, and known topo-IIα inhibitor, etoposide, respectively, the compounds were biotransformed in rat liver microsomes. LC-MS/MS and MALDI mass spectrometric techniques has been used for analysis. All three compounds were biotransformed into demethylated metabolites. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.026DOI Listing

Toward a better understanding of chronic kidney disease with complementary chromatographic methods hyphenated with mass spectrometry for improved polar metabolome coverage.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 26;1116:9-18. Epub 2019 Mar 26.

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland; Swiss Center of Human Applied Toxicology, University of Basel, Switzerland. Electronic address:

The prevalence of chronic kidney disease (CKD) is increasing worldwide. New technical approaches are needed to improve early diagnosis, disease understanding and patient monitoring, and to evaluate new therapies. Metabolomics, as a prime candidate in the field of CKD research, aims to comprehensively analyze the metabolic complexity of biological systems. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.031DOI Listing
May 2019
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Monitoring polysorbate hydrolysis in biopharmaceuticals using a QC-ready free fatty acid quantification method.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 26;1116:1-8. Epub 2019 Mar 26.

F. Hoffmann-La Roche, Grenzacherstrasse 124, 4070 Basel, Switzerland. Electronic address:

Hydrolysis of the non-ionic surfactant polysorbate upon long-term storage poses significant challenges to development of biopharmaceutical liquid formulations. Low concentrations of intact surfactant may compromise its protective properties and thus affect protein stability. In addition, accumulation of polysorbate hydrolysis products is increasingly put into context with the formation of visible and subvisible particulates based on the low solubility of the main degradation products. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232193027
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http://dx.doi.org/10.1016/j.jchromb.2019.03.030DOI Listing
May 2019
2 Reads

Lipidomic profiles disturbed by the internet gaming disorder in young Korean males.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 23;1114-1115:119-124. Epub 2019 Mar 23.

The Department of Psychiatry, Yonsei University College of Medicine, Seoul 03722, Republic of Korea; Institute of Behavioral Science in Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. Electronic address:

Internet Gaming Disorder (IGD) is characterized by uncontrollable and persistent playing of internet games despite the occurrence of negative consequences. Although there is a worldwide treatment demand, IGD still doesn't have an explicit biomarker. The primary goal of the study is to characterize lipidomic profiles specific to internet gaming disorder (IGD) based on liquid-chromatography Orbitrap mass-spectrometry (LC Orbitrap MS). Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.027DOI Listing
May 2019
2 Reads

Rapid determination of sulfonamides in chicken using two-dimensional online cleanup mode with three columns coupled to liquid chromatography-tandem mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 15;1114-1115:110-118. Epub 2019 Mar 15.

Zhejiang Institution of Food and Drug Control, Zhejiang, Hangzhou 310052, China. Electronic address:

A novel method based on online cleanup mode combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established. After an automated sample cleanup system with aqueous gel column, sulfonamides in chicken were detected in multiple reaction monitoring (MRM) mode. The total run time of the system, which included automated extraction, analytical chromatography and re-equilibration, was within 30 min. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183172
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http://dx.doi.org/10.1016/j.jchromb.2019.03.015DOI Listing
May 2019
9 Reads

Screening of Bifidobacterium strains with assignment of functional groups to bind with benzo[a]pyrene under food stress factors.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 20;1114-1115:100-109. Epub 2019 Mar 20.

College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083 Beijing, China; Key Laboratory of Forest Food Processing and Safety, Beijing, China. Electronic address:

The purpose of this study was to find the component of the Bifidobacterium cell wall more particularly the functional groups from peptidoglycan involved in the mechanism of binding with Benzo[a]pyrene. Additionally, the effect of different stress factors (acid, heat, alkaline, oxidative, osmotic, enzymatic, and detergent factors) on the functional group and the overall binding mechanism of Bifidobacterium with B[a]p were also evaluated. Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) were used to explain the binding mechanism of Bifidobacterium with B[a]p along with HPLC (high-performance liquid chromatography). Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.024DOI Listing
May 2019
2 Reads

Application of a label-free and domain-specific free thiol method in monoclonal antibody characterization.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 26;1114-1115:93-99. Epub 2019 Mar 26.

Analytical Development, Biogen Inc., 125 Broadway, Cambridge, MA 02142, United States of America. Electronic address:

Characterization of free thiol variants in antibody therapeutics is important for biopharmaceutical development, as the presence of free thiols may have an impact on aggregate formation, structural and thermal stability, as well as antigen-binding potency of monoclonal antibodies. Most current methods for free thiol quantification involve labeling of free thiol groups by different tagging molecules followed by UV, fluorescence or mass spectrometry (MS) detection. Here, we optimized a label-free liquid chromatography (LC)-UV/MS method for free thiol quantification at a subunit level and compared this method with two orthogonal and conventional approaches, Ellman's assay and peptide mapping with differential alkylation. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.032DOI Listing
May 2019
1 Read

Purification of peroxidase enzyme from radish species in fast and high yield with affinity chromatography technique.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 27;1114-1115:86-92. Epub 2019 Mar 27.

Atatürk University, Science Faculty, Chemistry Department, 25240 Erzurum, Turkey. Electronic address:

In this study, an effective single step affinity method is presented for purifying plant peroxidase (POD) enzymes from radish species. This method make possible to purify the enzymes in high yield and purity. Briefly, 10 different 4-amino benzohydrazide derivatives were synthesized and identified as new competitive POD inhibitors. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.035DOI Listing
May 2019
5 Reads

Urine and fecal samples targeted metabolomics of carobs treated rats.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 23;1114-1115:76-85. Epub 2019 Mar 23.

Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece; Biomic_Auth, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research of Aristotle University of Thessaloniki, Innovation Area of Thessaloniki, Thermi 57001, Greece.

Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition. The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions. Metabolomics-based analysis provides an ultimate tool, for the deciphering of nutritional intervention derived metabolic alterations. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.028DOI Listing
May 2019
1 Read

Fabrication of a ferrocene-based monolithic column with a network structure and its application in separation of protein and small molecules.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 15;1114-1115:71-75. Epub 2019 Mar 15.

Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China. Electronic address:

A novel ferrocene-based monolith with a network structure was fabricated via in situ free radical polymerization using vinyl ferrocene as the co-monomer within a stainless-steel column (50 × 4.6 mm i.d. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.016DOI Listing
May 2019
2.729 Impact Factor

Use of UHPLC-QTOF-MS/MS with combination of in silico approach for distributions and metabolites profile of flavonoids after oral administration of Niuhuang Shangqing tablets in rats.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 21;1114-1115:55-70. Epub 2019 Mar 21.

School of Pharmacy, Nanchang University, Nanchang 330006, China.

Niuhuang Shangqing tablet (NHSQT), a well-known traditional Chinese medicine preparation, has been used as an over- the- counter drug for the treatment of headache, dizziness in China. The flavonoids are the main active components in NHSQT, however, there have no reports about their distribution and metabolic fate in vivo after oral administration of NHSQTs so far. An novel UHPLC-QTOF-MS/MS method combined with in silico approach was applied to identify the flavonoids and metabolites profiling in biological samples following oral administration NHSQTs for the first time. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.021DOI Listing
May 2019
1 Read

Facile and sensitive determination of urinary mandelic acid by combination of metal organic frameworks with microextraction by packed sorbents.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 21;1114-1115:45-54. Epub 2019 Mar 21.

Department of Biostatistics, School of Public Health and Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

In this study, two hybrid metal organic frameworks including MOF-5@ FeO-NH and MOF-5@ SBA-15 for the first time were synthetized and combined with microextraction by packed sorbent (MEPS) to extract of mandelic acid (MA) from urine samples. The synthetized sorbents were characterized using FE-SEM, XRD and FT-IR techniques. The important parameters in MEPS procedure including sample volume, extraction draw_discard cycles, elution solvent volume and desorption draw-eject cycles were optimized using response surface methodology (RSM) with central composite design (CCD). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232193003
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http://dx.doi.org/10.1016/j.jchromb.2019.03.023DOI Listing
May 2019
2 Reads

A cost-effective method for purification and characterization of human urinary albumin.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 20;1114-1115:31-44. Epub 2019 Mar 20.

Northwest Lipid Metabolism and Diabetes Research Laboratories (NWRL), Department of Medicine, University of Washington, 401 Queen Anne Ave North, Seattle, WA 98109, USA.

We describe a simplified approach for the purification and characterization of urinary albumin, a key biomarker currently used for understanding the onset and prognosis of microalbuminuria. Urinary albumin was purified from human urine collected from diabetic kidney disease patients by using 2-stage tangential flow filtration process and set of column chromatography steps. The relative molecular mass of urinary albumin is 66,871 Da (SYNAPT G2 High Definition Mass Spectrometry System). Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.020DOI Listing
May 2019
1 Read
2.729 Impact Factor

Magnetic solid phase extraction with carbon-coated FeO nanoparticles coupled to HPLC-UV for the simultaneous determination of losartan, carvedilol, and amlodipine besylate in plasma samples.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 20;1114-1115:24-30. Epub 2019 Mar 20.

Department of Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran.

In this paper, a chemical coprecipitation method was used for the synthesis of FeO magnetic nanoparticles. The FeO magnetic nanoparticles were then modified by carbon via a simple hydrothermal method by using glucose. The synthesized FeO and carbon-coated FeO (C/FeO) magnetic nanoparticles were characterized by diverse techniques such as XRD, FESEM, EDX, FTIR, and BET. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.025DOI Listing

Targeted UPLC-MS/MS high-throughput metabolomics approach to assess the purine and pyrimidine metabolism.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 8;1113:98-106. Epub 2019 Mar 8.

Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan west Road, Tianjin 300193, China. Electronic address:

Purines and pyrimidines, the important components of DNA and RNA, are closely related to metabolic syndrome and disorder, such as renal disease, gout and diabetic nephropathy etc. Given the importance of the biological significance of purines and pyrimidines, it is necessary to further develop a rapid and sensitive method for practical detection of a large-scale analyses. In this study, based on 96-well solid phase extraction plates-ultra performance liquid chromatography-triple quadrupole mass spectrometry (SPE-UPLC-QqQ-MS/MS), a novel approach for simultaneous determination of 23 purines and pyrimidines in biological samples was developed. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.008DOI Listing
April 2019
2 Reads

Aqueous two phase based selective extraction of mannose/glucose specific lectin from Indian cultivar of Pisum sativum seed.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 19;1114-1115:13-23. Epub 2019 Mar 19.

Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, Mangalore 575025, India. Electronic address:

Pisum sativum lectin (Psl) being a high-value protein has marked its application in the biomedical and therapeutic field. Aqueous two phase extraction (ATPE) was implemented as a selective partitioning technique for the partial purification of Psl from its seeds. PEG/citrate based biodegradable aqueous two phase system (ATPS) was screened and the factors such as the type and concentration of citrate salts, molar mass and concentration of polyethylene glycol (PEG), tie line length (TLL) and additive (NaCl) concentration, pH, crude load and volume ratio were studied for the selective partition of Psl. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.019DOI Listing

Immunoaffinity microcryogels for purification of transferrin.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 18;1114-1115:5-12. Epub 2019 Mar 18.

Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara, Turkey. Electronic address:

Immunoaffinity chromatography has a huge interest in the biomedical and biotechnological fields, in particular for one-step isolation, purification and removal of analyte compounds. In this study, uniform-sized microcryogels, a new type of cryogels, were synthesized using 2-hydroxyetyhl methacrylate and epoxy-group-containing monomer, glycidyl methacrylate for purification of a plasma protein, transferrin. Immunoaffinity microcryogels containing anti-Tf antibodies were characterized by Raman spectroscopy, Fourier transform infrared spectroscopy, optical microscopy, scanning electron microscopy, density measurements and swelling tests. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183137
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http://dx.doi.org/10.1016/j.jchromb.2019.03.017DOI Listing
May 2019
2 Reads

The study and application of analyte adduct based ionisation of propofol in the analysis with liquid chromatography-tandem mass spectrometry.

Authors:
Marek Dziadosz

J Chromatogr B Analyt Technol Biomed Life Sci 2019 May 18;1114-1115:1-4. Epub 2019 Mar 18.

Institute of Legal Medicine, Hannover Medical School (MHH), Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address:

The ionisation of propofol was studied on the basis of analyte adduct formation with the components of the mobile phase. The identification of an appropriate propofol adduct ion generated in an acetate salt based buffer in the negative electrospray ionisation mode made the propofol LC-MS/MS analysis with two multiple reaction monitoring experiments possible without analyte derivatisation. Since a product ion of the deprotonated analyte was also used in the detection, these two fragmentation experiments were performed with different precursor ions. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.018DOI Listing

Development and validation of a UPLC-UV method for the quantification of thiopurine methyltransferase enzyme activity in human erythrocytes.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 14;1113:91-97. Epub 2019 Mar 14.

Service de Biochimie, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France; INSERM U1147, Centre Universitaire des Saints Pères, Paris, France; Université Paris Descartes, Paris, France; Service de Néphrologie, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France.

Thiopurines are drugs widely used for the treatment of autoimmune conditions, inflammatory bowel disease or acute lymphoblastic leukemia. Determination of thiopurine methyltransferase activity (TPMT), a major determinant of thiopurines toxicity, has been suggested before implementing thiopurine treatment. An ultraperformance liquid chromatography (UPLC) method was developed and validated for the quantification of TPMT enzyme activity based on the conversion of 6-mercaptopurine (6-MP) to 6-methylmercaptopurine (6-MMP) using S-adenosyl-L-methionine (SAM) as methyl donor in red blood cell lysates (RBC). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183138
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http://dx.doi.org/10.1016/j.jchromb.2019.03.014DOI Listing
April 2019
4 Reads

Establishment of a rapid and simple liquid chromatography tandem mass spectrometry method for measuring aldosterone in urine.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 14;1113:84-90. Epub 2019 Mar 14.

Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing 100730, China.

Aldosterone (ALD) measurement plays a critical role in screening and diagnosis of primary aldosteronism. A variety of liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for serum ALD have been developed. However, the few LC-MS/MS methods for 24 h urinary ALD that have been reported require tedious sample pretreatment procedure. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.012DOI Listing

A novel xanthine oxidase inhibitor WSJ-557 study on pharmacokinetics and tissue distribution in rats by UPLC-MS/MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 15;1113:77-83. Epub 2019 Mar 15.

Department of Pharmacy, the First Hospital of China Medical University, 155 Nanjing North Street, Shenyang 110001, Liaoning, China; School of Pharmaceutical Science, China Medical University, 77 Puhe Road, Shenyang 110122, Liaoning, China. Electronic address:

As a novel non-purine xanthine oxidase inhibitor, WSJ-557 is a potential drug for gout. To determine the WSJ-557 concentration in plasma and various tissues of rats, a fast and sensitive method was first established by the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in this paper. The liquid-liquid extraction of ethyl acetate was adopted for the sample preparation, and carbamazepine was taken as the internal standard. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.03.013DOI Listing
April 2019
3 Reads

A simple GC-MS method for the determination of diphenylamine, tolylfluanid propargite and phosalone in liver fractions.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 6;1113:69-76. Epub 2019 Mar 6.

Aix Marseille Univ, CNRS, LCE, Marseille, France.

In this study, an accurate and robust gas chromatography/mass spectrometry method was developed for quantitative analysis of diphenylamine, tolylfluanid, propargite and phosalone in liver fractions. Different injector parameters were optimized by an experimental design technique (central composite design). An optimal combination of injector temperature (°C), splitless time (min) and overpressure (kPa) values enabled to maximize the chromatographic responses. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15700232183120
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http://dx.doi.org/10.1016/j.jchromb.2019.03.005DOI Listing
April 2019
8 Reads