6,421 results match your criteria Journal of Biotechnology[Journal]


Type II flavoprotein monooxygenase PsFMO_A from the bacterium Pimelobacter sp. Bb-B catalyzes enantioselective Baeyer-Villiger oxidations with a relaxed cofactor specificity.

J Biotechnol 2019 Jan 28. Epub 2019 Jan 28.

Chair of Organic Chemistry I, Faculty of Chemistry, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany. Electronic address:

Microbial consortia, which degrade branched, long-chain hydrocarbons, can be regarded as a promising source of novel enzymes for the stereo- and regio-selective oxyfunctionalization of hydrocarbons. The hydrocarbon-degrading bacterium Pimelobacter sp. Bb-B was isolated from the consortium associated with the colonial hydrocarbon-excreting microalga Botryococcus braunii. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.011DOI Listing
January 2019
1 Read

CHO Genome Mining for Synthetic Promoter Design.

J Biotechnol 2019 Jan 28. Epub 2019 Jan 28.

Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield S1 3JD, UK. Electronic address:

Synthetic promoters are an attractive alternative for use in mammalian hosts such as CHO cells as they can be designed de novo with user-defined functionalities. In this study, we describe and validate a method for bioprocess-directed design of synthetic promoters utilizing CHO genomic sequence information. We designed promoters with two objective features, (i) constitutive high-level recombinant gene transcription, and (ii) upregulated transcription under mild hypothermia or late-stage culture. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.015DOI Listing
January 2019
5 Reads

High-yield production of D-1,2,4-butanetriol from lignocellulose-derived xylose by using a synthetic enzyme cascade in a cell-free system.

J Biotechnol 2019 Feb 28;292:76-83. Epub 2019 Jan 28.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China.

Approaches using metabolic engineering to produce D-1, 2, 4-butanetriol (BT) from renewable biomass in microbial systems have achieved initial success. However, due to the lack of incomplete understanding of the complex branch pathway, the efficient fermentation system for BT production was difficult to develop. Here we reconstituted a cell-free system in vitro using purified enzymes to produce BT from d-xylose. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.004DOI Listing
February 2019

Food-grade expression of an iron-containing acid urease in Bacillus subtilis.

J Biotechnol 2019 Jan 28. Epub 2019 Jan 28.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Jiangsu, Wuxi, 214122, China. Electronic address:

Enzymatic degradation of urea, the precursor of carcinogenic compound ethylcarbamate in rice wine, is always attractive. In the present study, we achieved high efficient production of Bacillus paralicheniformis iron-containing urease (Bp_Urease) in B. subtilis with the food-grade expression system. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.012DOI Listing
January 2019

Construction of stabilized (R)-selective amine transaminase from Aspergillus terreus by consensus mutagenesis.

J Biotechnol 2019 Jan 28;293:8-16. Epub 2019 Jan 28.

Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, PR China. Electronic address:

Amine transaminases are a class of efficient and industrially-desired biocatalysts for the production of chiral amines. In this study, stabilized variants of the (R)-selective amine transaminase from Aspergillus terreus (AT-ATA) were constructed by consensus mutagenesis. Using Consensus Finder (http://cbs-kazlab. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.007DOI Listing
January 2019
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Whole-cell cascade for the preparation of enantiopure β-O-4 aryl ether compounds with glutathione recycling.

J Biotechnol 2019 Jan 28;293:1-7. Epub 2019 Jan 28.

Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany. Electronic address:

Bacterial β-etherases and glutathione lyases are glutathione-dependent enzymes that catalyze the selective cleavage of β-O-4 aryl ether bonds found in lignin. Their glutathione (GSH) dependence is regarded as major limitation for their application in the production of aromatics from lignin polymer and oligomers, as stoichiometric glutathione amounts are required. Thus, recycling of the GSH cofactor by a NAD(P)H-dependent glutathione reductase was proposed previously. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.002DOI Listing
January 2019

Engineering Escherichia coli for respiro-fermentative production of pyruvate from glucose under anoxic conditions.

J Biotechnol 2019 Jan 26;293:47-55. Epub 2019 Jan 26.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center "Kurchatov Institute", 1-st Dorozhniy pr., 1, 117545, Moscow, Russia.

An Escherichia coli K-12 MG1655-derived strain was engineered for respiro-fermentative production of pyruvate from glucose under anoxic conditions, which is preferred for industrial-scale microbial synthesis of valuable chemicals. The pathways of anaerobic pyruvate dissimilation were blocked in the strain by the deletion of the ackA, pta, poxB, ldhA, adhE, and pflB genes. The phosphoenolpyruvate-dependent phosphotransferase system of glucose transport and phosphorylation was substituted by an alternative ATP-dependent system resulting from the overexpression of galP and glk upon deletion of ptsG. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.013DOI Listing
January 2019

Characterization of folding-sensitive nanobodies as tools to study the expression and quality of protein particle immunogens.

J Biotechnol 2019 Jan 26;293:17-23. Epub 2019 Jan 26.

Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435 (C1405BWE), Ciudad de Buenos Aires, Argentina. Electronic address:

Vaccination is as one of the most beneficial biopharmaceutical interventions against pathogens due to its ability to induce adaptive immunity through targeted activation of the immune system. Each vaccine needs a tailor-made set of tests in order to monitor its quality throughout the development and manufacturing. The analysis of the conformational state of protein nanoparticles is one of the key steps in vaccine quality control. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.001DOI Listing
January 2019

'Structural constraints in cyanobacteria-mediated whole-cell biotransformation of methoxylated and methylated derivatives of 2'-hydroxychalcone.

J Biotechnol 2019 Jan 26;293:36-46. Epub 2019 Jan 26.

Department of Analytical and Ecological Chemistry, Faculty of Chemistry, University of Opole, Oleska 48, 45-052, Opole, Poland. Electronic address:

Halophilic and freshwater strains of cyanobacteria representing the Oscillatoriales, Nostocales, Chroococcales, and Synechococcales orders of Cyanophyta were examined to determine (i) the resistance of their cultures when suppressed by the presence of exogenous methoxylated and methylated derivatives of 2'-hydroxychalcone, (ii) morphological changes in cells treated with the tested chalcones and, most importantly, (iii) whether these photoautotrophic microorganisms transform chalcone derivatives in a structure- or strain-dependent manner. The growth of cyanobacterial cultures depended on chalcone derivatives and the strain; nevertheless, trends for correlations between these parameters are difficult to determine. The exposure of cyanobacteria to the tested chalcones revealed severe membrane damage that was consistent with the disruption of membrane integrity. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.005DOI Listing
January 2019
1 Read

Deep eutectic solvents for redox biocatalysis.

J Biotechnol 2019 Jan 26;293:24-35. Epub 2019 Jan 26.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands. Electronic address:

Deep eutectic solvents (DES) are a class of neoteric solvents used in multiple applications amongst which biocatalytic processes. Due to its simple preparation, low cost and inherent biodegradable properties, its use as a non-volatile biocompatible co-solvent with both whole cells and isolated enzymes has displayed increased enzyme activity and stability translating to higher product conversions and a surprising higher enantioselectivity in a range of biotransformations. This review lays out the latest updates on the use of DES in redox biocatalytic reactions. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.018DOI Listing
January 2019
1 Read
2.871 Impact Factor

N-Alkyl-α-amino acids in Nature and their biocatalytic preparation.

J Biotechnol 2019 Jan 26;293:56-65. Epub 2019 Jan 26.

Advanced Manufacturing Technologies, GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK. Electronic address:

N-Alkylated-α-amino acids are useful building blocks for the pharmaceutical and fine chemical industries. Enantioselective methods of N-alkylated-α-amino acid synthesis are therefore highly valuable and widely investigated. While there are a variety of chemical methods for their synthesis, they often employ stoichiometric quantities of hazardous reagents such as pyrophoric metal hydrides or genotoxic alkylating agents, whereas biocatalytic routes can provide a greener and cleaner alternative to existing methods. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193001
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http://dx.doi.org/10.1016/j.jbiotec.2019.01.006DOI Listing
January 2019
5 Reads

Bioinspired genetic engineering of supramolecular assembled formate dehydrogenase with enhanced biocatalysis activities.

J Biotechnol 2019 Feb 26;292:50-56. Epub 2019 Jan 26.

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, 361005, China; Collaborative Innovation Center of Chemistry for Energy Materials, Xiamen University, Xiamen, 361005, China. Electronic address:

A bioinspired strategy for the synthesis of supramolecular and biocatalytical materials was developed base on protein-protein supramolecular interaction and genetic engineering. Formate dehydrogenase (FDH) and its functional fragments were separately fused to form a multi-function domain. The fusion proteins and functional fragments self-assembled into the expanded and controllable supramolecular interaction networks. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.017DOI Listing
February 2019

Cold hydrolysis of cassava pulp and its use in simultaneous saccharification and fermentation (SSF) process for ethanol fermentation.

J Biotechnol 2019 Feb 26;292:57-63. Epub 2019 Jan 26.

Department of Biotechnology, Khon Kaen University, Khon Kaen, 40002 Thailand. Electronic address:

The present study investigated cold hydrolysis of cassava pulp (CP) and the use of cold hydrolysis with simultaneous saccharification and fermentation (SSF) for ethanol production. Cold hydrolysis of 100 g-CP/L at 50 °C for 2 h, followed by at 30 °C for 72 h resulted in the production of 71.5 ± 1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193000
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http://dx.doi.org/10.1016/j.jbiotec.2019.01.003DOI Listing
February 2019
2 Reads

Highly active nanobiocatalysis in deep eutectic solvents via metal-driven enzyme-surfactant nanocomposite.

J Biotechnol 2019 Feb 26;292:39-49. Epub 2019 Jan 26.

National Engineering Research Center for Coatings, CNOOC Changzhou Paint and Coatings Industry Research Institute Co., Ltd, Changzhou 213016, China.

Metal-driven papain-surfactant nanocomposite (PA@MSNC), a novel soft nanobiocatalyst, was successfully prepared via one-pot self-assembly technique in aqueous solution for the biosynthesis of N-(benzyloxycarbonyl)-L-alanyl-L-glutamine (Z-Ala-Gln) dipeptide in deep eutectic solvents (DESs). The metal-driven self-assembly process generated PA@MSNC as nanospheres of ˜130 nm in diameter, with high protein loading and relative enzyme activity of 420 mg/g and 80% (4270 U/g protein), respectively. PA@MSNC showed high apparent substrate affinity and catalytic efficiency. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.010DOI Listing
February 2019
1 Read

Efficient production of aggregation prone 4-α-glucanotransferase by combined use of molecular chaperones and chemical chaperones in Escherichia coli.

J Biotechnol 2019 Feb 26;292:68-75. Epub 2019 Jan 26.

College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.

In this study, a combined optimization strategy, based on co-expression of molecular chaperones and supplementation of osmolytes, was used to reduce the formation of inclusion bodies and enhance the expression of the soluble form of 4-α-glucanotransferase. The 4-α-glucanotransferase yield was enhanced by co-expression with pGro7 and supplementation of trimetlylamine oxide. Subsequently, the effects of process conditions (temperature, inducer concentration, and arabinose concentration) on cell growth and 4-α-glucanotransferase production were also investigated in shake flasks. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.014DOI Listing
February 2019

Inhibition of recombinant enzyme 3-hydroxy-3-methylglutaryl-CoA reductase from Candida glabrata by α-asarone-based synthetic compounds as antifungal agents.

J Biotechnol 2019 Feb 26;292:64-67. Epub 2019 Jan 26.

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala, Col. Sto. Tomás s/n, 11340 Mexico City, Mexico. Electronic address:

Due to increasing resistance of Candida species to antifungal drugs, especially azoles, new drugs are needed. The proposed compounds 3 and 4 are analogous to α-asarone (2), a naturally occurring potent inhibitor of HMGR with hypolipidemic and antifungal activity. We used the recombinant enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase of Candida glabrata (CgHMGR) as a model to test the effectiveness of the test compounds. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.008DOI Listing
February 2019

Thermal and operational deactivation of Aspergillus fumigatus β-glucosidase in ethanol/water pretreated wheat straw enzymatic hydrolysis.

J Biotechnol 2019 Feb 19;292:32-38. Epub 2019 Jan 19.

Chemical Engineering and Materials Department, Faculty of Chemistry, Universidad Complutense, 28040, Madrid, Spain. Electronic address:

The stabilization effects on a novel commercial β-glucosidase preparation from Aspergillus fumigatus during saccharification of ethanol-water pretreated wheat straw were analysed in comparison to this enzyme stability during cellobiose hydrolysis. For this purpose, the kinetics of β-glucosidase residual activity during cellobiose hydrolysis from 40 till 70 °C were studied, resulting in the fitting of a first-order partial deactivation model. Furthermore, a subsequent fitting of a kinetic model including this first-order deactivation equation and a Michaelis-Menten equation with double competitive inhibition by glucose and uncompetitive inhibition by cellobiose to released glucose was successful. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.009DOI Listing
February 2019

Directed expression of halophilic and acidophilic β-glucosidases by introducing homologous constitutive expression cassettes in marine Aspergillus niger.

J Biotechnol 2019 Feb 18;292:12-22. Epub 2019 Jan 18.

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

The hydrolysis step by β-glucosidase (BGL) is generally recognized as the major limiting step in cellulose degradation and the BGLs with prominent enzymatic properties are of great importance for efficient utilization of lignocellulosic biomass. In order to identify some salt-tolerant BGLs, two BGL genes were cloned from marine Aspergillus niger ZJUBE-1 genome. Then two bgl expression cassettes driven by gpdA promoter were respectively transformed into marine A. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.015DOI Listing
February 2019
2 Reads

Biosynthesis in vitro of bacillamide intermediate-heterocyclic AlaCys by heterologous expression of nonribosomal peptide synthetase (NRPS).

J Biotechnol 2019 Feb 11;292:5-11. Epub 2019 Jan 11.

Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Bacillamide C, a potential natural antialgae active compound, is produced by Bacillus atrophaeus C89 derived from marine sponge Dysidea avara. A nonribosomal peptide synthetase (NRPS) cluster is hypothesized to be involved in the biosynthesis of bacillamide C. The NRPS with a domain string of A1-PCP1-Cy-A2-PCP2-C can be divided into three functional modules. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.024DOI Listing
February 2019
6 Reads

Genome mining reveals the origin of a bald phenotype and a cryptic nucleocidin gene cluster in Streptomyces asterosporus DSM 41452.

J Biotechnol 2019 Feb 11;292:23-31. Epub 2019 Jan 11.

Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs University, Freiburg, Germany. Electronic address:

Streptomyces asterosporus DSM 41452 is a producer of the polyketide annimycin and the non-ribosomal depsipeptide WS9326A. This strain is also notable for exhibiting a bald phenotype that is devoid of spores and aerial mycelium when grown on solid media. Based on the similarity of the 16S rRNA sequence to Streptomyces calvus, the only known producer of the fluorometabolite nucleocidin, the genome of S. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.016DOI Listing
February 2019

Overexpression of RCK1 improves acetic acid tolerance in Saccharomyces cerevisiae.

J Biotechnol 2019 Feb 4;292:1-4. Epub 2019 Jan 4.

Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. Electronic address:

Mixed sugars derived from lignocellulosic biomass can be converted into biofuels and chemicals by engineered microorganisms, but toxic fermentation inhibitors produced from harsh depolymerization processes of lignocellulosic biomass pose a critical challenge for economic production of biofuels and chemicals. Unlike other fermentation inhibitors generated from sugar degradation, acetic acid is inevitably produced from acetylated hemicellulose, and its concentrations in cellulosic hydrolysates are substantially higher than other fermentation inhibitors. The aim of this study was to identify novel genetic perturbations for improved acetic acid tolerance in Saccharomyces cerevisiae. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.013DOI Listing
February 2019
5 Reads

Preparation of a reversible soluble-insoluble β-d-Glucosidase with perfect stability and activity.

J Biotechnol 2019 Feb 4;291:46-51. Epub 2019 Jan 4.

College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Rebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymaticcatalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemicalmodification, represents a simple but effective route. Here, a class of highly stable and active reversible soluble-insoluble β-d-Glucosidase (E-CB-ALD 5K) was prepared using mPEG-ALD (methoxy-polyethylene glycol aldehyde, molecular weight: 5k) as a site-specific modifier and Eudragit L-100(polyacrylic resin) as a carrier. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193000
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http://dx.doi.org/10.1016/j.jbiotec.2018.12.014DOI Listing
February 2019
4 Reads

Microbial production of glycolate from acetate by metabolically engineered Escherichia coli.

J Biotechnol 2019 Feb 4;291:41-45. Epub 2019 Jan 4.

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

Escherichia coli was metabolically engineered to synthesize glycolate using acetate as the carbon source. The native glyoxylate bypass pathway was reinforced by the overexpression of isocitrate lyase and isocitrate dehydrogenase kinase/phosphatase. Glyoxylate/hydroxypyruvate reductase was overexpressed to convert glyoxylate to glycolate. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.012DOI Listing
February 2019
1 Read
2.871 Impact Factor

Corrigendum to "Whole-genome sequence of purple non-sulfur bacteria, Rhodobacter sphaeroides strain MBTLJ-8 with improved CO reduction capacity" [J Biotechnol 288./ (2018) 9-14].

J Biotechnol 2019 Jan;290:67

Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University,567 Baekje-daero, Deokjin-Gu Jeonju, Jeonbuk 54896, South Korea; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea. Electronic address:

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.012DOI Listing
January 2019

Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining.

J Biotechnol 2019 Feb 26;291:26-34. Epub 2018 Dec 26.

College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310012, China; Collaborative innovation center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310032, China. Electronic address:

(R)-Pantolactone (PL) is a key chiral intermediate for the synthesis of calcium (R)-pantothenate and (R)-panthenol used as food additives. The commercial production of (R)-pantothenate is performed by the resolution of racemic pantothenate, which is synthesized through an aldol condensation and a cyanation reaction. In this study, we investigated another synthetic method of (R)-pantothenate through the stereoselective reduction of ketopantoyl lactone (KPL) by aldo-keto reductase (AKR). Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.011DOI Listing
February 2019

A novel anti-cancer L19-interleukin-12 fusion protein with an optimized peptide linker efficiently localizes in vivo at the site of tumors.

J Biotechnol 2019 Feb 23;291:17-25. Epub 2018 Dec 23.

Philochem AG, Libernstrasse 3, 8112, Otelfingen, Switzerland. Electronic address:

Antibody-cytokine fusion proteins are a class of biopharmaceuticals, with the potential to modulate the activity of the immune system at the site of disease. The molecular format used to connect antibody moiety and cytokine payload can have a profound influence on biological activity and pharmacokinetic properties. The optimization of fusion protein format is particularly challenging for heterodimeric cytokines, since various molecular arrangements can be considered. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.004DOI Listing
February 2019
1 Read

Metabolic engineering of Corynebacterium glutamicum for the fermentative production of halogenated tryptophan.

J Biotechnol 2019 Feb 20;291:7-16. Epub 2018 Dec 20.

Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany. Electronic address:

Halogenated compounds, like 7-chloro-l-tryptophan, are important intermediates or components of bioactive substances relevant for the pharmaceutical, chemical and agrochemical industries. About 20% of all pharmaceutical small molecule drugs and around 30% of all active compounds in agrochemistry are halogenated. Chemical halogenation procedures usually are characterized by the use of hazardous or even highly toxic chemicals. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.008DOI Listing
February 2019
3 Reads
2.871 Impact Factor

Targeted delivery of calreticulin to ED-A fibronectin leads to tumor-growth retardation.

J Biotechnol 2019 Jan 20;290:53-58. Epub 2018 Dec 20.

Department of Applied Biosciences, Swiss Federal Institute of Technology (ETH Zürich), Vladimir-Prelog-Weg 4, CH-8093, Zurich, Switzerland. Electronic address:

We report the design and characterization of novel fusion proteins, consisting of the F8 antibody and of murine calreticulin (Calr). The F8 antibody recognizes the alternatively-spliced ED-A domain of fibronectin, an extracellular matrix component found in most tumor types, while calreticulin has previously been described as an "eat-me" signal for dendritic cells and phagocytes. Four fusion proteins, differing in antibody formats and peptide linkers, were produced in mammalian cells, purified to homogeneity and tested in vitro and in vivo. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.007DOI Listing
January 2019
4 Reads

One-pot synthesis of a 3-thiazolidine through combination of an Asinger-type multi-component-condensation reaction with an enzymatic imine reduction.

J Biotechnol 2019 Feb 20;291:35-40. Epub 2018 Dec 20.

Chair of Organic Chemistry I, Faculty of Chemistry, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany. Electronic address:

Addressing the combination of heterocyclic chemistry and biocatalysis, a one-pot synthesis of a 3-thiazolidine by means of an initial Asinger-type multi-component-condensation reaction under formation of a 3-thiazoline and a subsequent enzymatic reduction of this cyclic imine is reported. The one-pot process is conducted in a sequential mode and without the need for a spatial compartmentalization of the single reaction steps. For the biocatalytic reduction an imine reductase was used in combination with a glucose dehydrogenase and D-glucose for in situ-cofactor recycling. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.009DOI Listing
February 2019

Bacterial cellulose as a support for yeast immobilization - Correlation between carrier properties and process efficiency.

J Biotechnol 2019 Feb 20;291:1-6. Epub 2018 Dec 20.

West Pomeranian University of Technology in Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Immunology, Microbiology and Physiological Chemistry, Piastów 45, 70-311 Szczecin, Poland. Electronic address:

The aim of the current study was to analyze physicochemical properties of bacterial cellulose (BC) produced by Komagataeibacter xylinus for various periods of time in stationary conditions with regard to its potential as a carrier for yeast (Saccharomyces cerevisiae and Yarrowia lipolytica) immobilization, and subsequently to correlate the relationship between these properties and the efficiency of the immobilization process. Physicochemical properties of BC, depending on the time of its biosynthesis, were as follows: surface area 7.21-11. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.010DOI Listing
February 2019

Substrate specificity of novel GH16 endo-β-(1→3)-galactanases acting on linear and branched β-(1→3)-galactooligosaccharides.

J Biotechnol 2019 Jan 18;290:44-52. Epub 2018 Dec 18.

Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet 207, DK-2800 Kgs. Lyngby, Denmark. Electronic address:

Arabinogalactan proteins are proteoglycans located in the plant cell wall. Most arabinogalactan proteins are composed of carbohydrate moieties of β-(1→3)-galactan main chains with β-(1→6)-galactan side chains terminated by other glycans. In this study, three novel endo-β-(1→3)-galactanases were identified and the substrate specificity was further studied using well-defined galactan oligomers. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.006DOI Listing
January 2019
2.871 Impact Factor

Enantioselective synthesis of enantiopure β-amino alcohols via kinetic resolution and asymmetric reductive amination by a robust transaminase from Mycobacterium vanbaalenii.

J Biotechnol 2019 Jan 13;290:24-32. Epub 2018 Dec 13.

Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.

Chiral β-amino alcohols are very important chiral building block for preparing bioactive compounds for use in pharmaceutical and fine chemical industries. Synthesis of chiral β-amino alcohols by transaminase is big challenging due to the strict substrate specificities and very low activity of the enzyme. In this work, a (R)-selective ω-transaminase (MVTA) from Mycobacterium vanbaalenii was employed as a biocatalyst for the first time for the synthesis of chiral β-amino alcohol via kinetic resolution and asymmetric reductive amination. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656183071
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http://dx.doi.org/10.1016/j.jbiotec.2018.12.003DOI Listing
January 2019
7 Reads

Disruption of por1 gene in Candida utilis improves co-production of S-adenosylmethionine and glutathione.

J Biotechnol 2019 Jan 13;290:16-23. Epub 2018 Dec 13.

School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China. Electronic address:

The por1 gene encoding one of the mitochondrial porin channels in C. utilis CCTCC M 209298 was disrupted using a homologous recombination method. The co-production of S-adenosylmethionine (SAM) and glutathione (GSH) in the mutant C. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.005DOI Listing
January 2019
1 Read

Comparison and implementation of different control strategies for improving production of rHSA using Pichia pastoris.

J Biotechnol 2019 Jan 11;290:33-43. Epub 2018 Dec 11.

Department of Chemical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, India. Electronic address:

Recombinant protein production by various host organisms is strictly regulated, which is determined mainly by host cell genetics and maintenance of process variables within desirable limits in a reactor. Since the process dynamics typically exhibit nonlinear interactions amongst the critical process variables, the commonly employed controllers are suboptimal for control of most bioprocesses. In this article, we present the implementation of an augmented controller for improving the production of recombinant human serum albumin (rHSA) using Pichia pastoris. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.002DOI Listing
January 2019

Highly efficient production of chiral amines in batch and continuous flow by immobilized ω-transaminases on controlled porosity glass metal-ion affinity carrier.

J Biotechnol 2019 Feb 11;291:52-60. Epub 2018 Dec 11.

Van' t Hoff Institute for Molecular Sciences, HIMS-Biocat & HetCat, University of Amsterdam, Science Park 904, 1098 XH, The Netherlands. Electronic address:

In this study, two stereocomplementary ω-transaminases from Arthrobacter sp. (AsR-ωTA) and Chromobacterium violaceum (Cv-ωTA) were immobilized via iron cation affinity binding onto polymer-coated controlled porosity glass beads (EziG). The immobilization procedure was studied with different types of carrier materials and immobilization buffers of varying compositions, concentrations, pHs and cofactor (PLP) concentrations. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.12.001DOI Listing
February 2019

Optimization of production of vesicular stomatitis virus (VSV) in suspension serum-free culture medium at high cell density.

J Biotechnol 2019 Jan 30;289:144-149. Epub 2018 Nov 30.

Department of Bioprocess Engineering, National Research Council Canada, Building Montreal, Montréal, Canada; Department of Bioengineering McGill University, Montréal, Canada.

During the last decade, oncolytic viruses such as vesicular stomatitis virus (VSV) have gained tremendous popularity as efficient vaccines for infectious diseases as well as for the treatment of cancer. Our laboratory has developed two stable cell lines, 293SF-3F6 (derived from HEK293A cells) and SF-BMAdR cells (a variant of A549 that expresses the E1 region of human adenovirus). These two cell lines were adapted to grow efficiently in suspension culture and in serum-free medium. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.023DOI Listing
January 2019
4 Reads

Enhancement of the Brenneria sp. levansucrase thermostability by site-directed mutagenesis at Glu located at the "-TEAP-" residue motif.

J Biotechnol 2019 Jan 29;290:1-9. Epub 2018 Nov 29.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address:

Levansucrase (EC 2.1.4. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.021DOI Listing
January 2019
9 Reads

Lactobacillus reuteri NAD(P)H oxidase: Properties and coexpression with propanediol-utilization enzymes for enhancing 3-hydroxypropionic acid production from 3-hydroxypropionaldehyde.

J Biotechnol 2019 Jan 29;289:135-143. Epub 2018 Nov 29.

Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address:

Lactobacillus reuteri metabolizes glycerol through propanediol-utilization (Pdu) pathway to 1,3-propanediol (1,3-PD) via 3-hydroxypropionaldehyde (3-HPA) as intermediate. In the resting cells, the oxidized co-factor obtained in the reaction is regenerated by simultaneous oxidation of 3-HPA to 3-hydroxypropionic acid (3-HP) using propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL) and propionate kinase (PduW). We have earlier shown that the use of resting cells of recombinant Escherichia coli expressing the oxidative pathway gives the highest theoretical yield of 1 mol 3-HP per mol 3-HPA but is limited by cofactor depletion. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.010DOI Listing
January 2019
2 Reads

An enhanced regeneration strategy to improve microbial control and prolong resin lifetime for Protein A resin in large-scale monoclonal antibody (mAb) purification.

J Biotechnol 2019 Jan 28;289:118-125. Epub 2018 Nov 28.

CMC Biologics, Teva Pharmaceuticals USA, Inc, PA, United States.

Effective Protein A column regeneration is important in therapeutic monoclonal antibody (mAb) production for product quality and process economics. To design a robust and effective regeneration strategy, resin compatibility, microbial inactivation efficiency and cleaning effectiveness are three major considerations. Regenerating Protein A column using acidic (e. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.022DOI Listing
January 2019
3 Reads

pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose.

J Biotechnol 2019 Jan 26;290:10-15. Epub 2018 Nov 26.

Department of Chemical and Biomolecular Engineering, DOE Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Institute of Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. Electronic address:

Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH's and lipid production enhanced at more acid pH's. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.10.012DOI Listing
January 2019
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Genetic modification of the carotenoid pathway in the red yeast Xanthophyllomyces dendrorhous: Engineering of a high-yield zeaxanthin strain.

J Biotechnol 2019 Jan 26;289:112-117. Epub 2018 Nov 26.

Institute of Molecular Biosciences, Goethe-University, Frankfurt/M, Germany. Electronic address:

The red yeast Xanthophyllomyces dendrorhous was genetically engineered for high-yield accumulation of the carotenoid zeaxanthin. Initially, an astaxanthin hyper-producing mutant was used to generate a β-carotene synthesizing transformant by inactivation of the astaxanthin synthase gene. Subsequently, a bacterial β-carotene hydroxylase gene was genome integrated to establish β-carotene to zeaxanthin conversion. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.019DOI Listing
January 2019

Modification of phage display technique for improved screening of high-affinity binding peptides.

J Biotechnol 2019 Jan 26;289:88-92. Epub 2018 Nov 26.

Department of Bionanotechnology, Graduate School, Hanyang University-ERICA, Ansan 15588, Republic of Korea. Electronic address:

The phage display technique is a combinatorial technology in which random peptides are displayed on the surface of the phage; it is widely used to identify high-affinity peptides that bind to a target protein. However, this technique presents several problems due to non-specific binding of the phages and steric hindrance caused by blocking agents. To overcome these problems, we tested two modified methods and compared their screening performance with that of the conventional method. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.020DOI Listing
January 2019
9 Reads

Engineering diverse eubacteria promoters for robust Gene expression in Streptomyces lividans.

J Biotechnol 2019 Jan 24;289:93-102. Epub 2018 Nov 24.

State key Lab of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Due to the lack of powerful gene regulation elements, the engineering development of Streptomyces is often limited. Here, we disclosed that the heterologous σ -dependent promoters, which have been reported as inefficient tools for gene expression in Streptomyces, could be efficiently recognized by Streptomyces housekeeping factor σ. Therefore, an effective strategy was developed to engineer these promoters for robust gene expression in Streptomyces by fusing them with optimized 5'-untranslation regions (5'-UTRs). Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.017DOI Listing
January 2019
11 Reads

A fluorescent 3D cell culture assay for high throughput screening of cancer drugs down-regulating survivin.

J Biotechnol 2019 Jan 22;289:80-87. Epub 2018 Nov 22.

William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, Ohio, 43210, USA. Electronic address:

Survivin, a member of inhibitor of apoptosis family, is currently undergoing intensive investigations as a promising cancer marker due to its overexpression in multiple tumor tissues and close relationship with chemotherapy resistance. In this study, a novel 3D survivin promoter assay was developed, using enhanced green fluorescent protein (EGFP) as the reporter to assess survivin promoter activity for cancer drug screening. Breast cancer MCF-7 cells were engineered to express EGFP controlled by a human survivin promoter and a CMV promoter, respectively. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.018DOI Listing
January 2019
9 Reads

Stereoselective synthesis of a key chiral intermediate of (S)-Rivastigmine by AKR-GDH recombinant whole cells.

J Biotechnol 2019 Jan 20;289:64-70. Epub 2018 Nov 20.

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, People's Republic of China. Electronic address:

Herein, three aldo/ketoreductases (AKRs) were obtained and used to prepare N- ethyl-methyl-carbamic acid-3-[(1S)-hydroxy-ethyl]-phenyl ester ((S)-NEMCA-HEPE), which is a key intermediate of (S)-Rivastigmine. To avoid the usage of extra cofactors, the recombinant whole-cells containing AKRs and glucose dehydrogenase (GDH) were constructed and applied in the reduction reaction. The excellent conversion of 83. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.016DOI Listing
January 2019
5 Reads

Separate production of hydrogen and methane from cassava wastewater with added cassava residue under a thermophilic temperature in relation to digestibility.

J Biotechnol 2019 Feb 20;291:61-71. Epub 2018 Nov 20.

Department of Industrial Chemistry and Textile Technology, Faculty of Science, Maejo University, Chiang Mai, 50290, Thailand. Electronic address:

In this research, the separate production of hydrogen (H) and methane (CH) from cassava wastewater with added cassava residue was investigated using a two-stage upflow anaerobic sludge blanket (UASB) system under thermophilic temperature (55 °C) in a continuous mode of operation and steady state condition. The two-stage UASB system was operated under an optimum chemical oxygen demand (COD) loading rate of 10.29 kg/md (based on the total volume of both bioreactors) of the cassava wastewater with different concentrations of added cassava residue. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.015DOI Listing
February 2019

Setting-up a fast and reliable cytokinin biosensor based on a plant histidine kinase receptor expressed in Saccharomyces cerevisiae.

J Biotechnol 2019 Jan 20;289:103-111. Epub 2018 Nov 20.

EA 2106 Biomolécules et Biotechnologies Végétales, Université de Tours, F-37200 Tours, France. Electronic address:

Cytokinins (CK) have been extensively studied for their roles in plant development. Recently, they also appeared to ensure crucial functions in the pathogenicity of some bacterial and fungal plant pathogens. Thus, identifying cytokinin-producing pathogens is a prerequisite to gain a better understanding of their role in pathogenicity. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.013DOI Listing
January 2019
10 Reads

Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems.

J Biotechnol 2019 Jan 19;289:126-134. Epub 2018 Nov 19.

Department of Biocatalysis, Institute of Catalysis and Petrochemistry (ICP) CSIC, Campus UAM, Cantoblanco, 28049, Madrid, Spain. Electronic address:

Different immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL) exhibited different properties for the ethanolysis of high oleic sunflower oil in solvent-free systems. TLL immobilized by interfacial adsorption on octadecyl (C-18) supports lost its 1,3-regioselectivity and produced more than 99% of ethyl esters. This reaction was influenced by mass-transfer limitations. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.014DOI Listing
January 2019
18 Reads

The immobilization of Candida rugosa lipase on the modified polyethersulfone with MOF nanoparticles as an excellent performance bioreactor membrane.

J Biotechnol 2019 Jan 17;289:55-63. Epub 2018 Nov 17.

Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran.

In this study, the modified nanocomposite membrane of polyethersulfone (PES) with NH-MIL-101(Cr) as a metal-organic framework (MOF) is exploited for Candida rugosa lipase (CRL) immobilization. To that end, the various amounts of NH-MIL-101(Cr) nanoparticles are blended into PES casting solution to fabricate ultrafiltration membrane via phase inversion technique. The incorporation efficiency of NH-MIL-101(Cr) nanoparticles on the membrane morphology is investigated using various techniques, namely atomic force microscopy (AFM), X-ray diffraction (XRD), and contact angle goniometry. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.011DOI Listing
January 2019
13 Reads

Multicopy integrants of crt genes and co-expression of AMP deaminase improve lycopene production in Yarrowia lipolytica.

J Biotechnol 2019 Jan 15;289:46-54. Epub 2018 Nov 15.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address:

Lycopene has been broadly studied in recent decades due to its health benefits including cancer prevention, anti-atherogenic and anti-obesity effects, and modulation of the immune system. To obtain efficient synthesis of lycopene, extensive researches have been conducted in various microbial cells, including Yarrowia lipolytica, to heterologously produce lycopene using various genetic and metabolic engineering methods. In this study, the effects of copy numbers of lycopene synthesis genes, a variety of key central metabolic genes (especially AMP deaminase-encoding gene AMPD), and 5-L fermenter cultivation on lycopene production in Y. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2018.11.009DOI Listing
January 2019
11 Reads