6,478 results match your criteria Journal of Biotechnology[Journal]


Detection of cell-free, exosomal and whole blood mitochondrial DNA copy number in plasma or whole blood of patients with serous epithelial ovarian cancer.

J Biotechnol 2019 Apr 16. Epub 2019 Apr 16.

Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Ovarian tumor is one of the leading causes of cancer among women. Patients are diagnosed at an advanced stage, usually. There is a need for new specific and sensitive biomarkers. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.015DOI Listing

Predictive accuracy of the breast cancer genetic risk model based on eight common genetic variants: The BACkSIDE study.

J Biotechnol 2019 Apr 16. Epub 2019 Apr 16.

Division of Oncology, Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava (JFMED UK), Slovakia.

Breast cancer (BC) development is caused by the interaction of environmental and genetic factors. At least 90 susceptible genetic variants with different population penetration and incidence have been associated with BC. This paper therefore analysed the individual discrimination power of 8 low penetrant common genetic variants and calculated the predictive accuracy of the genetic risk model. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193012
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http://dx.doi.org/10.1016/j.jbiotec.2019.04.014DOI Listing
April 2019
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On the critical evaluation and confirmation of germline sequence variants identified using massively parallel sequencing.

J Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Institute for Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia; Geneton Ltd., Bratislava, Slovakia. Electronic address:

Although massively parallel sequencing (MPS) is becoming common practice in both research and routine clinical care, confirmation requirements of identified DNA variants using alternative methods are still topics of debate. When evaluating variants directly from MPS data, different read depth statistics, together with specialized genotype quality scores are, therefore, of high relevance. Here we report results of our validation study performed in two different ways: 1) confirmation of MPS identified variants using Sanger sequencing; and 2) simultaneous Sanger and MPS analysis of exons of selected genes. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.013DOI Listing
April 2019
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Inhibitory effect of Bacillus velezensis on biofilm formation by Streptococcus mutans.

J Biotechnol 2019 Apr 12;298:57-63. Epub 2019 Apr 12.

Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon 22012, Republic of Korea; Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea. Electronic address:

Streptococcus mutans plays a key role in the development of dental caries and promotes the formation of oral biofilm produced by glucosyltransferases (GTFs). Bacillus velezensis K68 was isolated from traditional fermented foods and inhibits biofilm formation mediated by S. mutans. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.009DOI Listing
April 2019
1 Read

PEGAylated graphene oxide/superparamagnetic nanocomposite as a high-efficienc loading nanocarrier for controlled delivery of methotrexate.

J Biotechnol 2019 Apr 12. Epub 2019 Apr 12.

Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran.

Polymer-coated nanocarriers play an important role in targeted drug delivery. The use of polymers such as polyethylene glycol increases stability, biocompatibility, and blood circulation time of the drug, and may consequently improve the success of drug delivery. In the present work, a simple approach has been reported for synthesizing polyethylene glycol bis amin (PEGA) functionalized graphene oxide/iron oxide nanocomposite as a remarkable unit for loading drugs. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.006DOI Listing
April 2019
2 Reads

Significance of Liquid Biopsy in Glioblastoma - A Review.

J Biotechnol 2019 Apr 12. Epub 2019 Apr 12.

University of Debrecen, Faculty of Medicine, Department of Human Genetics, Hungary.

Glioblastoma (GBM) is the most common and most aggressive primary malignant brain tumor with a 16-24-months overall survival time (OS). Effective management is hindered by intratumoral heterogeneity, a characteristic trait of GBM which results in subpopulations of cells with altered therapeutic responsiveness, different invasiveness and growth potential. Correct initial molecular profiling of the tumor, as well as following its molecular biological changes are further impeded by the intracranial location of the tumors, hence the risks of surgical interventions. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.011DOI Listing

Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications.

J Biotechnol 2019 Apr 10;298:35-44. Epub 2019 Apr 10.

Université de Bordeaux/Bordeaux INP, ENSCBP, 16 avenue Pey-Berland, Pessac, 33607, France; CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629, LCPO), Pessac, France.

Elastin-like polypeptides (ELPs) are biocompatible-engineered polypeptides, with promising interest in tissue engineering due to their intrinsic biological and physical properties, and their ease of production. The IKVAV (Ile-Lys-Val-Ala-Val) laminin-1 sequence has been shown to sustain neuron attachment and growth. In this study, the IKVAV adhesion sequence, or a scrambled VKAIV sequence, were incorporated by genetic engineering in the structure of an ELP, expressed in Escherichia coli and purified. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.010DOI Listing
April 2019
8 Reads

Target genes for plant productivity improvement.

Authors:
B Nowicka

J Biotechnol 2019 Apr 9;298:21-34. Epub 2019 Apr 9.

Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland. Electronic address:

The use of chemical fertilizers and pesticides, as well as the development of high-yielding varieties enabled substantial increase in crop productivity during the 20th century. However, the increase in yield over the last two decades has been slower. It is thought that further improvement in productivity of the major crop species using traditional cultivation methods is limited. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.008DOI Listing
April 2019
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Increased fermentative adenosine production by gene-targeted Bacillus subtilis mutation.

J Biotechnol 2019 Apr 8;298:1-4. Epub 2019 Apr 8.

College of Biotechnology and Pharmaceutical Engineering, Bioenergy Research Institute, Nanjing Tech University, Nanjing, 211816, China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing, 211816, China; Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture, Nanjing, 211816, China. Electronic address:

Adenosine, which is produced mainly by microbial fermentation, plays an important role in the therapy of cardiovascular disease and has been widely used as an antiarrhythmic agent. In this study, guanosine 5'-monophosphate (GMP) synthetase gene (guaA) was inactivated by gene-target manipulation to increase the metabolic flux from inosine 5'-monophosphate (IMP) to adenosine in B. subtilis A509. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.007DOI Listing
April 2019
1 Read

Enhancement of galactose consumption rate in Saccharomyces cerevisiae CEN.PK2-1 by CRISPR Cas9 and adaptive evolution for fermentation of Kappaphycus alvarezii hydrolysate.

J Biotechnol 2019 May 5;297:78-84. Epub 2019 Apr 5.

Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea. Electronic address:

Ethanol ferrmentation of Kappaphycus alvarezii hydrolysates was performed using wild-type (WT) Saccharomyces cerevisiae CEN.PK2-1, hexokinase 2 deleted (Δhxk2) and adapted strain on high galactose concentrations. The WT and Δhxk2 strains produced 8. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.012DOI Listing
May 2019
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Constructing an efficient salicylate biosynthesis platform by Escherichia coli chromosome integration.

J Biotechnol 2019 Apr 5;298:5-10. Epub 2019 Apr 5.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

Salicylate (SA) is an important platform chemical widely used in cosmetic and pharmaceutical industries. In this study, an efficient SA producing strain was constructed by step-by-step chromosome integration. First, the SA biosynthetic module controlled by promoters P or P was integrated into the chromosome of E. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.004DOI Listing
April 2019
1 Read

Expression of CD24 in plasma, exosome and ovarian tissue samples of serous ovarian cancer patients.

J Biotechnol 2019 Apr 5;298:16-20. Epub 2019 Apr 5.

Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

CD24 is a small molecular weight cell-surface protein and an independent marker for poor prognosis in the different type of cancers. We aimed to determine the expression of CD24 in plasma, exosomes and ovarian tissue samples of serous ovarian cancer patients. We collected tissue and blood samples from 21 cases of serous ovarian cancer and eight healthy controls. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.018DOI Listing
April 2019
2 Reads

Assessment of fed-batch cultivation strategies for an inducible CHO cell line.

J Biotechnol 2019 Apr 5;298:45-56. Epub 2019 Apr 5.

Department of Chemical Engineering, École Polytechnique de Montréal, P.O. Box 6079, succ. Centre-Ville, Montréal QC, H3C 3A7, Canada. Electronic address:

In order to maximize cell growth and productivity for an inducible CHO cell line expressing rituximab, various fed-batch culture strategies were investigated. In each case, the performance was evaluated for cultures induced at moderate and high cell density conditions (4 × 10 and 10 × 10 cells/mL) to assess the impact of the timing of induction. We first demonstrate the importance of starting the feeding process during the growth phase, as this translated into significantly improved integral of viable cells and antibody concentration, when compared to post-induction feeding only. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.005DOI Listing
April 2019
3 Reads

Quantitative RT-PCR-based miRNA profiling of blastemal Wilms' tumors from formalin-fixed paraffin-embedded samples.

J Biotechnol 2019 Apr 5;298:11-15. Epub 2019 Apr 5.

Department of Human Genetics, Faculty of Medicine, University of Debrecen, 1 Egyetem tér, 4032, Debrecen, Hungary. Electronic address:

Blastemal Wilms' tumors are associated with poor chemo-responsiveness and an adverse prognosis. Our aim was to contribute to the miRNA profiling of the disease, while demonstrating the value of archived formalin-fixed, paraffin-embedded (FFPE) samples as miRNA sources. MiRNA was extracted from tumor and normal tissues of 8 patients diagnosed with blastemal Wilms' tumor in Hungary. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.001DOI Listing

Liquid biopsy for predictive mutational profiling of solid cancer: The pathologist's perspective.

Authors:
Gábor Méhes

J Biotechnol 2019 May 3;297:66-70. Epub 2019 Apr 3.

Department of Pathology, Faculty of Medicine, University of Debrecen, Nagyerdei krt 98, H-4032, Debrecen, Hungary. Electronic address:

The pathological diagnostics of cancer - based on the histological features - is today increasingly completed by molecular profiling at variable depth in an almost evident fashion. Predictive information should cover potential therapeutic targets and/or major resistance mechanisms the nature of which is subject of alteration during the course of the treatment. Mutational profiling recently became technically available by the analysis of circulating free DNA obtained following non-invasive peripheral blood or body fluid sampling. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.002DOI Listing
May 2019
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Circulating epithelial-mesenchymal transition-associated miRNAs are promising biomarkers in ovarian cancer.

J Biotechnol 2019 May 3;297:58-65. Epub 2019 Apr 3.

Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. Electronic address:

Ovarian cancer is the fifth most common cause of cancer death among women that is mostly due to the difficulty of early diagnosis. Circulating miRNAs proved to be reliable biomarkers in various cancers. We screened 9 miRNAs, which are involved in epithelial-mesenchymal transition, in the plasma samples of patients with malignant (n = 28) or non-malignant (n = 12) ovarian tumors and disease-free healthy volunteers (n = 60) by qRT-PCR. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.04.003DOI Listing

Comparison of plasma and urinary microRNA-483-5p for the diagnosis of adrenocortical malignancy.

J Biotechnol 2019 May 30;297:49-53. Epub 2019 Mar 30.

2nd Department of Internal Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi str. 46., 1088 Budapest, Hungary; MTA-SE Molecular Medicine Research Group, Hungarian Academy of Sciences and Semmelweis University, Szentkirályi str. 46., 1088 Budapest, Hungary. Electronic address:

Introduction: Minimally invasive circulating microRNAs might be used for the preoperative differentiation of adrenocortical carcinoma (ACC) and adrenocortical adenoma (ACA). So far, the best blood-borne microRNA biomarker of ACC is circulating hsa-miR-483-5p. The expression of urinary hsa-miR-483-5p as a non-invasive marker of malignancy and its correlation with plasma hsa-miR-483-5p, has not been investigated, yet. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.017DOI Listing
May 2019
3 Reads

Enhancing the synthesis of latex clearing protein by different cultivation strategies.

J Biotechnol 2019 May 30;297:32-40. Epub 2019 Mar 30.

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149, Münster, Germany.

In this study, we improved the synthesis of the latex clearing protein from Gordonia polyisoprenivorans VH2 (Lcp1), a key enzyme for the initial cleavage of the rubber backbone. Cultivations using a recombinant strain of Escherichia coli were optimized to overcome poor solubility of Lcp1 and improve the production yields. Different cultivation temperatures and agitation rates were evaluated in the process to demonstrate their impact on the solubility of Lcp1. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.019DOI Listing
May 2019
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Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase.

J Biotechnol 2019 May 27;297:71-77. Epub 2019 Mar 27.

Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010, Graz, Austria. Electronic address:

With more than 50,000 members, terpenoids are one of the most important classes of natural products and show an enormous diversity. Due to their unique odors and specific bioactivities they already find wide application in the flavor, fragrance and pharma industries. Since most terpenoids can only be obtained by natural product extraction, the discovery of biosynthetic genes for the generation of terpene diversity becomes increasingly important. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.006DOI Listing
May 2019
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Identification of miR-146a and miR-196a-2 single nucleotide polymorphisms at patients with high-grade serous ovarian cancer.

J Biotechnol 2019 May 20;297:54-57. Epub 2019 Mar 20.

Department of Obstetrics and Gynecology, University of Debrecen, Debrecen Hungary. Electronic address:

MicroRNAs play an essential role in the regulation of gene expression and tumor development. Single nucleotide polymorphism (SNP) can be observed in miRNAs and could influence gene expression. We aimed to identify miR-146a rs2910164 and miR-196a-2 rs11614913 polymorphisms in ovarian cancer patients and controls. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.016DOI Listing
May 2019
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Monitoring and control strategies for inclusion body production in E. coli based on glycerol consumption.

J Biotechnol 2019 Apr 20;296:75-82. Epub 2019 Mar 20.

Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria; Research Division Biochemical Engineering, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.

The Gram-negative bacterium E. coli is the host of choice for the production of a multitude of recombinant proteins in industry. Generally, cultivation is easy, media are cheap and a high product titer can be obtained. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.014DOI Listing

Gateway binary vectors with organelle-targeted fluorescent proteins for highly sensitive reporter assay in gene expression analysis of plants.

J Biotechnol 2019 May 19;297:19-27. Epub 2019 Mar 19.

Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, Japan; Bioresources Science, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan. Electronic address:

Fluorescent proteins are valuable tools in the bioscience field especially in subcellular localization analysis of proteins and expression analysis of genes. Fusion with organelle-targeting signal accumulates fluorescent proteins in specific organelles, increases local brightness, and highlights the signal of fluorescent proteins even in tissues emitting a high background of autofluorescence. For these advantages, organelle-targeted fluorescent proteins are preferably used for promoter:reporter assay to define organ-, tissue-, or cell-specific expression pattern of genes in detail. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.015DOI Listing
May 2019
10 Reads

Effects of a 15-amino-acid isoform of amyloid- β expressed by silkworm pupae on B6C3-Tg Alzheimer's disease transgenic mice.

J Biotechnol 2019 Apr 18;296:83-92. Epub 2019 Mar 18.

Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences, Zhejiang Sci-Tech University, 5 Second Avenue, Xiasha Higher Education Zone, Hangzhou, 310018, China. Electronic address:

Silkworms are an economically important insect.Silkworm pupae are also a nutrient-rich food and can be used as a pharmaceutical intermediate.The N-terminus of Aβ includes 1-15 amino acid residues with a B cell surface antigen that is necessary to produce antibody and prevent the adverse reactions observed in response to the full Aβ42 peptide. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.013DOI Listing

Light-dependent growth kinetics enable scale-up of well-mixed phototrophic bioprocesses in different types of photobioreactors.

J Biotechnol 2019 May 18;297:41-48. Epub 2019 Mar 18.

Technical University of Munich, Institute of Biochemical Engineering, Garching, Germany; Technical University of Munich, TUM AlgaeTec Center, Ottobrunn, Germany. Electronic address:

Scaling of phototrophic bioprocesses can be extremely challenging especially when reactor types in the considered scales differ. In this study, the mean integral photon flux density was used to transfer light-dependent growth kinetics of Nannochloropsis salina 40.85 and Nannochloropsis gaditana 2. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.003DOI Listing

In situ microscopy as online tool for detecting microbial contaminations in cell culture.

J Biotechnol 2019 Apr 18;296:53-60. Epub 2019 Mar 18.

Institute of Technical Chemistry, Leibniz University Hannover, 30167, Hanover, Germany.

Microbial contamination in mammalian cell cultures causing rejected batches is costly and highly unwanted. Most methods for detecting a contamination are time-consuming and require extensive off-line sampling. To circumvent these efforts and provide a more convenient alternative, we used an online in situ microscope to estimate the cell diameter of the cellular species in the culture to distinguish mammalian cells from microbial cells depending on their size. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.011DOI Listing

Comparative biodegradation of all chlorinated phenols by the microalga Scenedesmus obliquus - The biodegradation strategy of microalgae.

J Biotechnol 2019 Apr 16;296:61-68. Epub 2019 Mar 16.

Department of Biology, University of Crete, Voutes University Campus, GR-70013 Heraklion, Greece. Electronic address:

This work presents the comparative biodegradation of all chlorinated phenolic compounds by the green alga Scenedesmus obliquus and determines the microalgal bioenergetic strategy. The microalga manages its energy reserves rationally by investing them, either on growth or on the biodegradation of the toxic compound. The microalga seems to follow two distinct detoxification strategies. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.010DOI Listing

Assembly of TALE-based DNA scaffold for the enhancement of exogenous multi-enzymatic pathway.

J Biotechnol 2019 Apr 15;296:69-74. Epub 2019 Mar 15.

Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, Hunan, 410073, China.

Synthetic scaffold systems, which exhibit enzyme clustering effect, have been considered as an important parallel approach for metabolic flux control and pathway enhancement. Here, we described an improved DNA-based scaffold system for synthetic tri-enzymatic pathway in Escherichia coli. With plasmid DNA serving as scaffold and exogenous enzymes fused with rationally designed transcription activator-like effectors (TALEs), our approach successfully clustered three TALE-fused enzymes and significantly increased the production of a mevalonate-producing tri-enzymatic pathway with the optimized scaffold structure and plasmid copy number. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.008DOI Listing

Reducing recombinant protein expression during CHO pool selection enhances frequency of high-producing cells.

J Biotechnol 2019 Apr 15;296:32-41. Epub 2019 Mar 15.

National Research Council Canada, 6100 Royalmount Avenue, Montréal, QC H4P 2R2, Canada; Département de biochimie et médecine moléculaire, Faculté de médecine, Université de Montréal, QC, Canada. Electronic address:

Chinese hamster ovary (CHO) cells are the most widely used mammalian host for industrial-scale production of monoclonal antibodies (mAbs) and other protein biologics. Isolation of rare high-producing CHO cell lines from heterogeneous populations of stable transfectants is a daunting task and delays the process of manufacturing of novel biologics. A variety of factors that contribute to the low frequency of high-producing clones have been described; however, the impact of metabolic burden and other stresses (eg. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.009DOI Listing

Functional horseradish peroxidase-streptavidin chimeric proteins prepared using a silkworm-baculovirus expression system for diagnostic purposes.

J Biotechnol 2019 May 15;297:28-31. Epub 2019 Mar 15.

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan; Division of Biotechnology, Center for Future Chemistry, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan. Electronic address:

Rapid, convenient, sensitive detection methods are of the utmost importance in analytical tools. Enzyme-based signal amplification using horseradish peroxidase (HRP) is commonly implemented in clinical diagnostics kits based on enzyme-linked immunosorbent assay (ELISA), by which the limit of detection is greatly improved. Herein we report the design and preparation of recombinant fusion proteins comprising HRP and streptavidin (Stav), in which HRP was fused to either the N- or C-terminus of Stav ((HRP)-Stav or Stav-(HRP), respectively) using a baculovirus-silkworm expression system. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.007DOI Listing
May 2019
3 Reads

Design of a highly thermostable hemicellulose-degrading blend from Thermotoga neapolitana for the treatment of lignocellulosic biomass.

J Biotechnol 2019 Apr 16;296:42-52. Epub 2019 Mar 16.

Dipartimento di Biotecnologie, Università di Verona, Strada Le Grazie 15, 37134, Verona, Italy. Electronic address:

The biological conversion of lignocellulose into fermentable sugars is a key process for the sustainable production of biofuels from plant biomass. Polysaccharides in plant feedstock can be valorized using thermostable mixtures of enzymes that degrade the cell walls, thus avoiding harmful and expensive pre-treatments. (Hyper)thermophilic bacteria of the phylum Thermotogae provide a rich source of enzymes for such industrial applications. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.005DOI Listing

Synthetic Salicylic acid inducible recombinant promoter for translational research.

J Biotechnol 2019 May 14;297:9-18. Epub 2019 Mar 14.

Division of Plant and Microbial Biotechnology, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India. Electronic address:

In the present study, we have developed an inter-molecularly shuffled caulimoviral promoter for protein over-expression by placing the Upstream Activation Sequence (UAS) of Figwort Mosaic Virus (FMV; -249 to -54) at the 5'-end of the Cassava Vein Mosaic Virus (CsVMV) promoter fragment 8 (CsVMV8; -215 to +166) to design a hybrid promoter; FUASCsV8CP. The FUASCsV8CP promoter exhibited approximately 2.1 and 2. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193007
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http://dx.doi.org/10.1016/j.jbiotec.2019.03.004DOI Listing
May 2019
7 Reads

Enhanced secretion of cyclodextrin glucanotransferase (CGTase) by Lactococcus lactis using heterologous signal peptides and optimization of cultivation conditions.

J Biotechnol 2019 Apr 13;296:22-31. Epub 2019 Mar 13.

Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. Electronic address:

In previous studies of Lactococcus lactis, the levels of proteins secreted using heterologous signal peptides were observed to be lower than those obtained using the signal peptide from Usp45, the major secreted lactococcal protein. In this study, G1 (the native signal peptide of CGTase) and the signal peptide M5 (mutant of the G1 signal peptide) were introduced into L. lactis to investigate the effect of signal peptides on lactococcal protein secretion to improve secretion efficiency. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.013DOI Listing
April 2019
1 Read

Production and formulation of a new low-cost biosurfactant to remediate oil-contaminated seawater.

J Biotechnol 2019 Apr 12;295:71-79. Epub 2019 Mar 12.

Catholic University of Pernambuco (UNICAP), Rua do Príncipe, n. 526, Boa Vista, 50050-900, Recife, Pernambuco, Brazil; Advanced Institute of Technology and Innovation (IATI), Rua Joaquim de Brito, n. 216, Boa Vista, 50070-280, Recife, Pernambuco, Brazil. Electronic address:

The aim of the present study was to produce biosurfactants using three bacterial strains (Pseudomonas cepacia CCT6659, Bacillus methylotrophicus UCP 1616 and Bacillus cereus UCP 1615) cultivated in mineral medium containing different carbon (glucose, sucrose, molasses and waste frying oil) and nitrogen [NHNO, (NH)SO, peptone, yeast extract and corn steep liquor] sources. B. cereus stood out as the best biosurfactant producer when inoculated with a 1. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.025DOI Listing

Upscale and characterization of lignin-modifying enzymes from Marasmiellus palmivorus VE111 in a bioreactor under parameter optimization and the effect of inducers.

J Biotechnol 2019 Apr 8;295:1-8. Epub 2019 Mar 8.

Enzymes and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Francisco Getúlio Vargas Street, 1130, 95070-560 Caxias do Sul, RS, Brazil.

Testing different pHs, dissolved oxygen concentrations and temperatures, plus the addition of inducers, to optimize ligninolytic enzyme activity, resulted in increased production of laccases, total peroxidases and manganese peroxidases on the order of 2.1-fold, 4.6-fold and 10-fold, respectively; laccases reached 6588 U/mL, total peroxidases reached 3533 U/mL and manganese peroxidase achieved 60 U/mL. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.002DOI Listing
April 2019
2 Reads

Carboxylated carbon nanomaterials in cell cycle and apoptotic cell death regulation.

J Biotechnol 2019 Apr 7;296:14-21. Epub 2019 Mar 7.

School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei City 110, Taiwan; Master Program in Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei City 110, Taiwan. Electronic address:

Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects of carbon nanomaterials toward human cells, and their modulations in cellular mechanism, are not fully understood. Herein, four types of carbon nanomaterials, include long and short carbon nanotubes and graphene nanosheets, at low and high concentrations, were functionalized and dispersed in the biocompatible buffer for assessment. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.005DOI Listing
April 2019
1 Read

Modification of lipase from Candida cylindracea with dextran using the borane-pyridine complex to improve organic solvent stability.

J Biotechnol 2019 Apr 7;296:1-6. Epub 2019 Mar 7.

Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.

In this study, a commercial lipase derived from Candida cylindracea was chemically modified with dextran by conjugating ε-amine in the lysine residue with the carbonyl residue in oxidized dextran using the borane-pyridine complex as a reducing agent to increase the hydrophilicity of the microenvironment around the lipase in the presence of organic solvents. The degree of modification (53.2%), amount of dextran (0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193007
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http://dx.doi.org/10.1016/j.jbiotec.2019.02.009DOI Listing
April 2019
5 Reads

Efficient biosynthesis of (R)-3-amino-1-butanol by a novel (R)-selective transaminase from Actinobacteria sp.

J Biotechnol 2019 Apr 7;295:49-54. Epub 2019 Mar 7.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China. Electronic address:

(R)-3-amino-1-butanol is a key intermediate of Dolutegravir for the treatment of HIV/AIDS and its green and efficient biosynthesis has attracted a great deal of attention. Transaminases are currently used as promising biocatalyst for the synthesis of chiral amines. However, many transaminases have (S)-specificity and (R)-selective transaminases were less exploited and studied, making the production of (R)-amines remain challenging. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193006
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http://dx.doi.org/10.1016/j.jbiotec.2019.02.008DOI Listing
April 2019
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Systematic identification of a neutral site on chromosome of Synechococcus sp. PCC7002, a promising photosynthetic chassis strain.

J Biotechnol 2019 Apr 7;295:37-40. Epub 2019 Mar 7.

Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Dalian National Laboratory for Clean Energy, Dalian, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:

Cyanobacteria based photosynthetic biomanufacturing is supposed to be one of the alternative routes for sustainable production of biofuels and biochemicals. Synechococcus sp. PCC 7002 is a promising cyanobacterial chassis strain possessing desired properties for scaled cultivation in future. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.007DOI Listing
April 2019
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Biomass and lipid production by Rhodococcus opacus PD630 in molasses-based media with and without osmotic-stress.

J Biotechnol 2019 May 7;297:1-8. Epub 2019 Mar 7.

Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Chatuchak, Bangkok 10900, Thailand; Center of Excellence on Petrochemical and Materials Technology, Bangkok, Thailand. Electronic address:

Rhodococcus opacus PD630 was used to produce biomass and lipids in molasses-based media with and without osmotic stress. In a 7-day aerobic batch culture at 30 °C, the biomass and lipid concentrations were maximized using an initial molasses concentration of 80 g/L and ammonium acetate (nitrogen source) concentration of 2.14 g/L. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.012DOI Listing

Hydroxytyrosol rich-mixture from olive mill wastewater and production of green products by feeding Rhodopseudomonas sp. S16-FVPT5 with the residual effluent.

J Biotechnol 2019 Apr 7;295:28-36. Epub 2019 Mar 7.

Department of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 1, 56122 Pisa, Italy.

This study disserts on the exploitation of olive mill wastewater (OMW) for the production of both bio-based poly-β-hydroxybutyrate (PHB) and hydrogen (H) by using the residual effluent as feedstock for growing purple bacteria after the recovery of hydroxytyrosol-rich mixtures. In particular, Rhodopseudomonas sp. S16-FVPT5 was fed with either the virgin OMW or dephenolized-OMW (d-OMW). Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.006DOI Listing

Effects of partial maize silage substitution with microalgae on viscosity and biogas yields in continuous AD trials.

J Biotechnol 2019 Apr 7;295:80-89. Epub 2019 Mar 7.

BIOENERGY 2020+ GmbH, Inffeldgasse 21b, A-8010 Graz, Austria; University of Natural Resources and Life Sciences, Department for Agrobiotechnology, Institute of Environmental Biotechnology, Konrad Lorenz Str. 20, A-3430 Tulln, Austria.

The microalga Acutodesmus obliquus was investigated as a feedstock in semi-continuously fed anaerobic digestion trials, where A. obliquus was co-digested with pig slurry and maize silage. Maize silage was substituted by both 10% and 20% untreated, and 20% ultrasonicated microalgae biomass on a VS (volatile solids) basis. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.004DOI Listing

Atmospheric and room temperature plasma (ARTP) mutagenesis enables xylitol over-production with yeast Candida tropicalis.

J Biotechnol 2019 Apr 7;296:7-13. Epub 2019 Mar 7.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi Jiangsu 214122, China. Electronic address:

Xylitol is a sugar alcohol that is used as a sweetener in food and confections. Industrially, xylitol is manufactured by chemical hydrogenation of d-xylose, which requires expensive separation and purification steps as well as high pressure and temperature. The microbial production of xylitol has been examined as an alternative to the chemical process. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.026DOI Listing
April 2019
2.871 Impact Factor

Transient manipulation of the expression level of selected growth rate correlating microRNAs does not increase growth rate in CHO-K1 cells.

J Biotechnol 2019 Apr 7;295:63-70. Epub 2019 Mar 7.

Austrian Centre of Industrial Biotechnology, Graz, Austria; University of Natural Resources and Life Sciences, Vienna, Austria. Electronic address:

Engineering of Chinese Hamster Ovary cells by manipulating microRNA (miRNA) expression levels has been shown to induce advantageous, desired phenotypes. Most of these studies so far were concerned with increasing productivity or reducing growth rate (with the implied intention of thus freeing cellular resources to also increase productivity). Here we evaluated the ability of growth correlating miRNAs to increase the growth rate of CHO-K1 cells by transient overexpression or knock down, respectively. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.011DOI Listing
April 2019
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Endoglucanase improve the growth of homofermentative Lactobacillus spp. in ensilages.

J Biotechnol 2019 Apr 7;295:55-62. Epub 2019 Mar 7.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, PR China. Electronic address:

Endoglucanase, an important component of cellulases, is used as additives in ensiling of forage crops. However, its detailed role is unclear in ensilages. In the present study, two endoglucanases Cel5 and Cel9 produced by strain Paenibacillus panacisoli SDMCC050309, previously isolated from ensiled corn stover, were identified in the cultures by microcrystalline cellulose absorption coupled with zymogram analysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193007
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http://dx.doi.org/10.1016/j.jbiotec.2019.02.010DOI Listing
April 2019
6 Reads

Multi-enzyme cascades as synthetic tool for biocatalysis.

J Biotechnol 2019 03 6;294:88. Epub 2019 Mar 6.

Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010, Graz, Austria. Electronic address:

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.001DOI Listing

Structure based virtual screening of novel noncompetitive antagonist  of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor.

J Biotechnol 2019 Apr 1;295:9-18. Epub 2019 Mar 1.

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) subtype ionotropic glutamate receptors are attractive antiepileptic targets responsible for mediating the majority of excitatory neurotransmission and plasticity. The noncompetitive antagonists obtain more and more attention as drug candidates for treatment of the neurological diseases involving excessive activity of AMPARs, due to they regulate AMPA receptors (AMPARs) activity independently of endogenous glutamate levels unlike the competitive antagonists. Development of novel AMPAR noncompetitive antagonists, which are safer and more efficacious than competitive antagonists, is highly under demand. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.023DOI Listing
April 2019
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Identification of a native promoter P for gene expression in Paenibacillus polymyxa.

J Biotechnol 2019 Apr 1;295:19-27. Epub 2019 Mar 1.

College of Life Sciences and Shandong Key Laboratory of Agricultural Microbiology and National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an, 271018, China.

Paenibacillus polymyxa is a rhizobacterium that has attracted substantial attention due to its ability to produce functional metabolites and promote plant growth. Metabolic and genetic improvements in this species will benefit research and other applications of the bacterium. However, a suitable gene expression system has not been established in this species. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.002DOI Listing
April 2019
5 Reads

Design of a multiepitopic Zaire ebolavirus protein and its expression in plant cells.

J Biotechnol 2019 Apr 28;295:41-48. Epub 2019 Feb 28.

Laboratorio de Biofarmacéuticos recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico. Electronic address:

The recent Ebola virus disease (EVD) outbreaks make the development of efficacious and low cost vaccines against Ebola virus (EBOV) an urgent goal. Multiepitopic vaccines allow a rational design rendering vaccines able to induce proper immune responses in terms of polarization and potency. In addition, the pathogen variants can be easily covered by including epitopes conserved among relevant isolates. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193005
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http://dx.doi.org/10.1016/j.jbiotec.2019.02.003DOI Listing
April 2019
10 Reads
2.871 Impact Factor

Biochemical characteristics and potential application of a novel ethanol and glucose-tolerant β-glucosidase secreted by Pichia guilliermondii G1.2.

J Biotechnol 2019 Mar 20;294:73-80. Epub 2019 Feb 20.

Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, São Paulo, Brazil. Electronic address:

β-glucosidases are glycoside hydrolases that-particularly those from filamentous fungi-have been extensively explored in cellulose fiber saccharification and wine quality improvement. However, these enzymes from yeast have been poorly studied. In this study, an ethanol-glucose tolerant β-glucosidase that is secreted by Pichia guilliermondii (current name Meyerozyma guilliermondii) was purified and characterized. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.02.001DOI Listing
March 2019
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Production of bioactive oligopeptide hydrolyzed by protease derived from aerial microalga Vischeria helvetica.

J Biotechnol 2019 Mar 14;294:67-72. Epub 2019 Feb 14.

Department of Chemistry and Life Science, School of Advanced Engineering, Kogakuin University, 2665-1 Nakano-machi, Hachioji, Tokyo 192-0015, Japan.

This study focused on a culture system of aerial microalgae with the decomposition of casein protein for obtaining bioactive compounds such as peptides with inhibitory activity against angiotensin-converting enzyme (ACE). The aerial microalga Vischeria helvetica exhibited growth in Bold's basal medium supplemented with casein protein as nitrogen source. The algal cells secreted protease and amino oxidase into the medium, and ammonium ions as a nitrogen source was produced by the conjugated-enzyme reaction. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.01.021DOI Listing