4,638 results match your criteria Journal of Bioscience and Bioengineering [Journal]


Activity-stability trade-off in random mutant proteins.

J Biosci Bioeng 2019 Apr 12. Epub 2019 Apr 12.

Department of Biomolecular Chemistry, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan. Electronic address:

In our previous study, we investigated the relationship between protein evolution and stability through the random mutational drift of an esterase from hyperthermophilic archaeon Sulfolobus tokodaii. The results revealed that evolvability, which is the appearance frequency of variants with higher activity than the parent protein, correlates with parental stability. This suggests that protein evolution that does not take stability into account does not make sense. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.017DOI Listing

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli.

J Biosci Bioeng 2019 Apr 12. Epub 2019 Apr 12.

Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, N13W8, Kitaku, Sapporo 060-8628, Japan.

Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (RuBisCO) generates 2-phosphoglycolate (2PG) as one of the metabolites from the Calvin-Benson-Bassham (CBB) cycle. In this study, we focused on the fact that glycolate (GL) derived from 2PG can be incorporated into the bacterial polyhydroxyalkanoate (PHA) as the monomeric constituent by using the evolved PHA synthase (PhaC1STQK). In this study, the function of the RuBisCO-mediated pathway for GL-based PHA synthesis was evaluated using Escherichia coli JW2946 with the deletion of glycolate oxidase gene (ΔglcD) as the model system. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.002DOI Listing

Expansion and differentiation of human iPS cells in a three-dimensional culture using hollow fibers and separation of the specific population by magnetic-activated cell sorting.

J Biosci Bioeng 2019 Apr 11. Epub 2019 Apr 11.

Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

In order to employ pluripotent stem cells in the field of regenerative medicine, it is necessary to establish a large-scale culture system for cell differentiation. We have developed a novel three-dimensional method for culturing human induced pluripotent stem (iPS) cells, using hollow fibers (HFs). The cells immobilized inside HFs can proliferate and form multicellular aggregates, capable of achieving a high cell density and promoting further spontaneous cell differentiation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183106
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http://dx.doi.org/10.1016/j.jbiosc.2019.03.014DOI Listing
April 2019
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Enzymatic and molecular characterization of an endoglucanase E from Clostridium cellulovorans 743B.

J Biosci Bioeng 2019 Apr 10. Epub 2019 Apr 10.

Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan. Electronic address:

Endoglucanase E (EngE) is a cellulosomal enzyme of the glycoside hydrolase family 5 generated by the cellulosome-producing bacterium Clostridium cellulovorans 743B. Although its basic activities and properties have been characterized, its substrate specificity, product range, and steady-state kinetics remain unclear. The current study prepared recombinant EngE (rEngE) and analyzed its substrate specificity and product range using thin layer chromatography. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.013DOI Listing

Time-resolved analysis of short term metabolic adaptation at dark transition in Synechocystis sp. PCC 6803.

J Biosci Bioeng 2019 Apr 10. Epub 2019 Apr 10.

Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

In photosynthetic organisms, such as cyanobacteria, ATP and NADPH are generated through the light reaction, and then are used for CO fixation in the dark reaction. As light intensity always fluctuates under natural conditions, balancing the cofactor regeneration and consumption is essential to maintain active CO fixation as well as for metabolic engineering of strains that produce biochemicals. In this study, a time-resolved metabolome analysis of Synechocystis sp. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723193016
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http://dx.doi.org/10.1016/j.jbiosc.2019.03.016DOI Listing
April 2019
3 Reads

Estimating effects of yeast extract compositions on Escherichia coli growth by a metabolomics approach.

J Biosci Bioeng 2019 Apr 9. Epub 2019 Apr 9.

Biotechnology and Food Chemistry Course Program, School of Regional Innovation and Social Design Engineering, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan. Electronic address:

Bioprocess stability depends on the variety of yeast extract, which varies from lot-to-lots and between brands, thereby leading to variable bacterial growth and productivity in manufacturing processes. As a model experiment for good stability of bioprocesses, Escherichia coli growth in media containing different brands of yeast extract was evaluated and predicted using component-profiling and multivariate data analysis (metabolomics approach). The components of yeast extract were extracted from media containing varying concentrations of yeast extract and analyzed using gas chromatography-mass spectrometer. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.012DOI Listing
April 2019
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Cellulase productivity of Trichoderma reesei mutants developed in Japan varies with varying pH conditions.

J Biosci Bioeng 2019 Apr 9. Epub 2019 Apr 9.

Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan. Electronic address:

The ascomycete Trichoderma reesei is known to produce a variety of cellulases and hemicellulases and the hyper-cellulolytic mutants of this fungus are useful as industrial cellulase producers. In Japan, PC-3-7, derived from the early mutant QM9414, is well-known as a cellulase hyperproducing mutant. In addition to the productivity of enzymes, the composition of secreted enzymes greatly influences biomass saccharification. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.005DOI Listing

Establishment of a transient CRISPR-Cas9 genome editing system in Candida glycerinogenes for co-production of ethanol and xylonic acid.

J Biosci Bioeng 2019 Apr 6. Epub 2019 Apr 6.

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address:

Candida glycerinogenes, an industrial yeast with excellent multi-stress tolerance, has been applied to glycerol production for decades. However, its genetic manipulation was limited by the absence of meiosis, the diploid genome, and the lack of molecular tools. We described here the implementation of a transient CRISPR-Cas9 system for efficient genome editing in C. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.009DOI Listing

Evaluation of synthetic gene encoding α-galactosidase through metagenomic sequencing of paddy soil.

J Biosci Bioeng 2019 Apr 5. Epub 2019 Apr 5.

Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 40227, Taiwan; Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung 40227, Taiwan. Electronic address:

Many genes of industrial relevance can be found in soil. In this study, metagenome sequencing of paddy soil was performed with 55.68 Gb sequences and 1,787,113 putative open reading frames (ORFs). Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.006DOI Listing
April 2019
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High-energy ball milling treatment of soybean for Bacillus thuringiensis culture media.

J Biosci Bioeng 2019 Apr 5. Epub 2019 Apr 5.

Instituto de Química Aplicada, Universidad del Papaloapan, Circuito Central 200, Parque Industrial, 68301 Tuxtepec, Oaxaca, Mexico. Electronic address:

Soybean meal has been intensively used as a substrate in culture media for several microorganisms. However, the fermentable sugar containing the soybean needs to be released from the solid matrix through different processes. Against this backdrop, the present study explores the use of high-energy ball milling as a one-step treatment method for expedited production of fermentable sugars of textured soybean. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.010DOI Listing

Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles.

J Biosci Bioeng 2019 Apr 5. Epub 2019 Apr 5.

Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

To develop a remote control system of transgene expression through localized cellular heating of magnetic nanoparticles, a heat-inducible transgene expression system was introduced into mammalian cells. Cells were labeled with magnetic nanoparticles and exposed to an alternating magnetic field. The magnetically labeled cells expressed the transgene in a monolayer and multilayered cell sheets in which cells were heated around the magnetic nanoparticles without an apparent temperature increase in the culture medium. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.008DOI Listing

Full-time dynamics of batch-wise enzymatic cycling system composed of two kinds of dehydrogenase mediated by NAD(P)H for mass production of chiral hydroxyl compounds.

Authors:
Tsuneo Yamane

J Biosci Bioeng 2019 Apr 4. Epub 2019 Apr 4.

Graduate School of Biological and Agricultural Sciences, Nagoya University, Furo-cho, Chikusa Ward, Nagoya 464-8601, Japan. Electronic address:

Enzymatic cycling system (coupled dehydrogenase-catalyzed biosystem being composed of two elementary enzymatic reactions mediated by NAD(P)H + NAD(P)) is industrially attractive for reducing prochiral carbonyl compounds to the corresponding chiral hydroxyl compounds. The reaction rate equation of the batch-wise biosystem was generally derived by ordered Bi Bi mechanism of two-substrate enzyme reaction on several reasonable assumptions. The rate equations of the batch-wise biosystem was generalized by transforming them into the dimensionless forms. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723193009
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http://dx.doi.org/10.1016/j.jbiosc.2019.03.004DOI Listing
April 2019
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Evaluation of meter-long monolithic columns for selected reaction monitoring mass spectrometry.

J Biosci Bioeng 2019 Apr 4. Epub 2019 Apr 4.

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan; JST, Core Research for Evolutional Science and Technology (CREST), 7 Goban-cho, Chiyoda-ku, Tokyo 102-0076, Japan; Kyoto Integrated Science & Technology Bio-Analysis Center, 134 Chudoji Minamimachi, Simogyo-ku, Kyoto 600-8813, Japan. Electronic address:

Proteome is extremely complex as many proteins with a large dynamic range are involved. Nano-liquid chromatography/mass spectrometry-based proteomics has made it possible to separate and identify thousands of proteins in one shot. Although the number of identified proteins in proteomics has significantly improved, it is necessary to increase detection sensitivity to clearly identify low-abundant proteins. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.03.001DOI Listing
April 2019
2 Reads

Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release.

J Biosci Bioeng 2019 Apr 3. Epub 2019 Apr 3.

Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan. Electronic address:

Manganese peroxidase (MnP) is a fungal heme-containing enzyme which oxidizes Mn to Mn, a diffusible and strong non-specific oxidant capable of attacking bulky phenolic substrates. Therefore, MnP is indispensable in the polymer and paper industries. Previous attempts of MnP expression in Escherichia coli resulted in the formation of inclusion bodies which required in vitro refolding. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.011DOI Listing

Guaiacol oxidation activity of herbivorous land crabs, Chiromantes haematocheir and Chiromantes dehaani.

J Biosci Bioeng 2019 Apr 2. Epub 2019 Apr 2.

Department of Environmental Science, Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.

The land crabs, Chiromantes haematocheir (Akate-gani) and Chiromantes dehaani (Kurobenkei-gani) inhabit seaside forests in Japan. The crabs mainly consume plant material and its detritus. Therefore, they are expected to possess the ability to degrade the major components of biomass, cellulose and lignin in order to digest plant materials. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.012DOI Listing

Exploring useful fermentation strategies for the production of hydroxyectoine with a halophilic strain, Halomonas salina BCRC 17875.

J Biosci Bioeng 2019 Mar 29. Epub 2019 Mar 29.

Graduate School of Biotechnology and Bioengineering, Yuan Ze University, No. 135 Yuan-Tung Rd., Chung-Li Dist., Taoyuan City 32003, Taiwan, ROC. Electronic address:

Hydroxyectoine, an ectoine derivative, is the most common compatible solute in halophilic microorganisms for resisting harsh environments. Compatible solutes can be utilized in fields such as cosmetics, medicine, and biochemistry. Moderately halophilic microorganisms produce much less hydroxyectoine as compared with ectoine. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183118
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http://dx.doi.org/10.1016/j.jbiosc.2019.02.015DOI Listing
March 2019
2 Reads

Liver-specific extracellular matrix hydrogel promotes liver-specific functions of hepatocytes in vitro and survival of transplanted hepatocytes in vivo.

J Biosci Bioeng 2019 Mar 29. Epub 2019 Mar 29.

Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.

A solubilized liver-specific extracellular matrix (L-ECM) substratum was obtained by decellularization of porcine liver using Triton X-100 and pepsin treatments. The L-ECM was able to immobilize hepatocyte growth factor at a high efficiency of 87%. L-ECM gelled spontaneously in a physiologically neutral environment. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.014DOI Listing

Proteomic study of bioactive peptides from tempe.

J Biosci Bioeng 2019 Mar 29. Epub 2019 Mar 29.

Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Darmaga, Bogor, West Java 16680, Indonesia. Electronic address:

Tempe is a traditional Indonesian fermented soybean mostly produced in small industries and sold locally throughout the country. Studies on the bioactive peptides in tempe are rare. Here, we studied bioactive peptides in samples from three tempe producers with different degrees of sanitation. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.019DOI Listing
March 2019
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Micro and ultrastructural changes monitoring during decellularization for the generation of a biocompatible liver.

J Biosci Bioeng 2019 Mar 20. Epub 2019 Mar 20.

College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea. Electronic address:

Decellularization of a whole organ is an attractive process that has been used to create 3D scaffolds structurally and micro-architecturally similar to the native one. Currently used decellularization protocols exhibit disrupted extracellular matrix (ECM) structure and denatured ECM proteins. Therefore, maintaining a balance between ECM preservation and cellular removal is a major challenge. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.007DOI Listing

Ultrasonically-guided flow focusing generates precise emulsion droplets for high-throughput single cell analyses.

J Biosci Bioeng 2019 Mar 20. Epub 2019 Mar 20.

Department of Chemical Engineering, The University of Kansas, Lawrence, KS 66044, USA; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66044, USA; Kansas Vaccine Institute, The University of Kansas, Lawrence, KS 66044, USA. Electronic address:

Emulsion-based techniques have dramatically advanced our understanding of single-cell biology and complex single-cell features over the past two decades. Most approaches for precise single cell isolation rely on microfluidics, which has proven highly effective but requires substantial investment in equipment and expertise that can be difficult to access for researchers that specialize in other areas of bioengineering and molecular biotechnology. Inspired by the robust droplet generation technologies in modern flow cytometry instrumentation, here we established a new platform for high-throughput isolation of single cells within droplets of tunable sizes by combining flow focusing with ultrasonic vibration for rapid and effective droplet formation. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.020DOI Listing

Investigation of relationship between sake-making parameters and sake metabolites using a newly developed sake metabolome analysis method.

J Biosci Bioeng 2019 Mar 15. Epub 2019 Mar 15.

National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Department of Molecular Biotechnology, Graduate School of Advanced Science of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-hiroshima, Hiroshima 739-8530, Japan.

We developed a sake metabolome analysis method using liquid chromatography-quadrupole/time-of-flight mass spectrometry to investigate the metabolome of various types of sakes and other alcohol beverages. Our method identified 198 compounds by comparison with standard metabolites. Using this method, we investigated the relationship between several sake-making parameters and sake metabolites by conducting combination experiments of these parameters using small-scale fermentation. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.013DOI Listing

Dense tracking of the dynamics of the microbial community and chemicals constituents in spontaneous wheat sourdough during two months of backslopping.

J Biosci Bioeng 2019 Mar 14. Epub 2019 Mar 14.

Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

Wheat sourdough is a common traditional fermented food that is produced worldwide. However, product quality of spontaneous sourdough is not easy to control because it depends on natural fermentation and backslopping, about which little is known, notably after ten backslopping steps. To this end, we tracked the spontaneous fermentation of three sourdoughs made from wheat flours during 32 backslopping steps for 60 days. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.006DOI Listing
March 2019
4 Reads

Pep-MS assay: Protease hydrolysis assay system using photo-cleavable peptide array and mass spectrometer.

J Biosci Bioeng 2019 Mar 9. Epub 2019 Mar 9.

Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan; Innovative Research Center for Preventative Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan. Electronic address:

Food processing technology such as protein hydrolysis using proteases has been receiving a lot of attention, and it is important to accurately understand the cleavage specificity of each protease for selecting a protease suited to aims. Although numerous methods have been reported to reveal the substrate specificity of proteases, there is no method to evaluate simply, quickly, reasonably, and accurately. This study set out to devise Pep-MS assay, a novel assay system that can be used to comprehensively clarify positions at which proteases cleave, by combining a mass spectrometer and a photo-cleavable peptide array. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.005DOI Listing

Modulation of culture medium confers high-specificity production of isopentenol in Bacillus subtilis.

J Biosci Bioeng 2019 Apr 26;127(4):458-464. Epub 2018 Oct 26.

Department of Biotechnology, National Institute of Technology Raipur, G.E. Road, Raipur 492010, Chhattisgarh, India. Electronic address:

Enthusiasm for mining isoprenoid-based flavors, pharmaceuticals, and nutraceuticals from GRAS (Generally Regarded as Safe) status microbial hosts has increased in the past few years due to the limitations associated with their plant-based extraction and chemical synthesis. Bacillus subtilis, a well-known GRAS microbe, is a promising alternative due to its fast growth rate and the ability to metabolize complex carbon sources. The study focused on the high-specificity production of isopentenol in B. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.10.002DOI Listing

Production of poly(3-hydroyxybutylate) by a novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 using alginate as a sole carbon source.

J Biosci Bioeng 2019 Mar 7. Epub 2019 Mar 7.

Laboratory of Marine Biotechnology and Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan. Electronic address:

A novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 that produces poly(3-hydroyxybutylate) (PHB) in the alginate-mineral salt (AMS) medium containing 1% (w/v) sodium alginate as a sole carbon source was isolated from a decayed brown seaweed litter. The yield of PHB produced by strain UMI-18 was 1. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.008DOI Listing
March 2019
2 Reads

Effect of extraction methods on the preparation of electrospun/electrosprayed microstructures of tilapia skin collagen.

J Biosci Bioeng 2019 Mar 1. Epub 2019 Mar 1.

College of Food Science & Technology, Shanghai Ocean University, No. 999 Huchenghuan Road, Shanghai 201306, China; Integrated Scientific Research Base on Comprehensive Utilization Technology for By-Products of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China, No. 999 Huchenghuan Road, Shanghai 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People's Republic of China, No. 999 Huchenghuan Road, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, China; State Key Laboratory of Molecular Engineering of Polymers (Fudan University), No. 220 Handan Road, Shanghai 200438, China. Electronic address:

Collagen plays a pivotal role in human physiological functions and extracted collagen has multiple potential applications. Tilapia skin can be applied to extract collagen for maximizing the profit of tilapia processing. Electrospinning/electrospraying is novel micro- and nano-techniques to fabricate microfibers and microspheres in a simple and easy way. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.004DOI Listing
March 2019
1 Read

Application of liposome membrane as the reaction field: A case study using the Horner-Wadsworth-Emmons reaction.

J Biosci Bioeng 2019 Mar 1. Epub 2019 Mar 1.

Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan. Electronic address:

The properties of the liposome membrane as a reaction field were investigated by focusing on the Horner-Wadsworth-Emmons reaction as a case study. Use of the liposomes existing in the gel phase resulted in the enhanced activity of the substrates and furnished the products with same E/Z stereoselectivity as in the liposome-free system. The membrane environment in the gel phase most likely assisted the formation of adducts that induced selective generation of the E-isomer. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.015DOI Listing

Biochemical characterization and mutational analysis of alanine racemase from Clostridium perfringens.

J Biosci Bioeng 2019 Feb 27. Epub 2019 Feb 27.

College of Life Science, Hebei Normal University, Shijiazhuang, 050024 Hebei, PR China. Electronic address:

Clostridium perfringens is a gram-positive, anaerobic, pathogenic bacterium that can cause a wide range of diseases in humans, poultry and agriculturally important livestock. A pyridoxal-5-phosphate-dependent alanine racemase with a function in the racemization of d- and l-alanine is an attractive drug target for C. perfringens and other pathogens due to its absence in animals and humans. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.003DOI Listing
February 2019
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Influence of α-1,3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis.

J Biosci Bioeng 2019 Feb 27. Epub 2019 Feb 27.

United Graduate School of Agricultural Science, Kagoshima University, 1-21-24 Korimoto, Kagoshima-shi, Kagoshima 890-8580, Japan; National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; Department of Bioscience and Biotechnology, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan. Electronic address:

Aspergillus luchuensis NBRC4314 recently underwent genome sequencing. We have not used the frequently used protoplast-polyethylene glycol (PEG) method but have used agrobacterium-mediated transformation (AMT) to genetically engineer this strain because it was difficult to generate protoplasts using commercial cell wall lytic enzymes. In this study, we initially investigated the various conditions for protoplast formation in A. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.018DOI Listing
February 2019
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Establishment of a high throughput-screening system for nucleoside deoxyribosyltransferase II mutant enzymes with altered substrate specificity.

J Biosci Bioeng 2019 Feb 23. Epub 2019 Feb 23.

Protein Science Laboratory of the Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address:

Nucleoside deoxyribosyltransferase II (NDT) catalyzes the transglycosylation reaction of the 2'-deoxyribose moiety between purine and/or pyrimidine bases and has been widely used in the synthesis of nucleoside analogs. The high specificity of NDT for 2'-deoxyribose limits its applications. Because 2'C- and/or 3'C-modified nucleosides have been widely used as antiviral or antitumour agents, improving the activity of NDT towards these modified nucleosides by protein engineering is an area of interest to the pharmaceutical industry. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.002DOI Listing
February 2019

Identification of metabolic engineering targets for improving glycerol assimilation ability of Saccharomyces cerevisiae based on adaptive laboratory evolution and transcriptome analysis.

J Biosci Bioeng 2019 Feb 22. Epub 2019 Feb 22.

School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan. Electronic address:

Glycerol, a by-product of biodiesel production, has been utilized as a raw material for bioproduction. Saccharomyces cerevisiae, which has been used as a host microorganism for bioproduction, possesses the metabolic pathways for glycerol assimilation, but it cannot grow on glycerol as a carbon source. In this study, we identified metabolic engineering targets to improve the glycerol assimilation ability of S. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.001DOI Listing
February 2019

Wound healing activity and mechanism of action of antimicrobial and lipopolysaccharide-neutralizing peptides from enzymatic hydrolysates of rice bran proteins.

J Biosci Bioeng 2019 Feb 21. Epub 2019 Feb 21.

Department of Materials Science and Technology, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan.

In our previous study, we identified multifunctional cationic peptides from enzymatic hydrolysates of rice bran proteins (RBPs) that have antimicrobial and lipopolysaccharide-neutralizing activities. In this study, we investigated the potential of the peptides RBP-LRR, RBP-EKL, and RBP-SSF to promote proliferation, angiogenesis (tube formation), and migration in human umbilical vein endothelial cells (HUVECs). To determine mechanisms of wound healing actions, angiogenic and migration-promoting activities of these peptides were evaluated following pretreatments of HUVECs with specific inhibitors. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.02.002DOI Listing
February 2019
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Biosynthesis of novel lactate-based polymers containing medium-chain-length 3-hydroxyalkanoates by recombinant Escherichia coli strains from glucose.

J Biosci Bioeng 2019 Feb 22. Epub 2019 Feb 22.

Department of Food and Health Sciences, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, Kumamoto 862-8502, Japan. Electronic address:

Novel lactate (LA)-based polymers containing medium-chain-length 3-hydroxyalkanoates (MCL-3HA) were produced in fadR-deficient Escherichia coli strains from glucose as the sole carbon source. The genes encoding LA and 3-hydroxybutyrate (3HB) monomers supplying enzymes [propionyl-CoA transferase (PCT), d-lactate dehydrogenase (D-LDH), β-ketothiolase (PhaA), and NADPH-dependent acetoacetyl-CoA reductase (PhaB)], MCL-3HA monomers supplying enzymes [(R)-3-hydroxyacyl-ACP thioesterase (PhaG) and (R)-3-hydroxyacyl (3HA)-CoA ligase] via fatty acid biosynthesis pathway, and modified polyhydroxyalkanoate (PHA) synthase [PhaC1(STQK)] of Pseudomonas sp. 61-3 were introduced into E. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.009DOI Listing
February 2019

Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives.

J Biosci Bioeng 2019 Feb 19. Epub 2019 Feb 19.

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki 12622, Giza, Egypt. Electronic address:

This study focused on kinetics of levan yield by Bacillus subtilis M, in a 150 L stirred tank bioreactor under controlled pH conditions. The optimized production medium was composed of (g/L): commercial sucrose 100.0, yeast extract 2. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723173110
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http://dx.doi.org/10.1016/j.jbiosc.2018.09.008DOI Listing
February 2019
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Harvesting of Scenedesmus acuminatus using ultrafiltration membranes operated in alternative feed directions.

J Biosci Bioeng 2019 Feb 18. Epub 2019 Feb 18.

Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory for Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China; Microalgae Biotechnology Center, China Electronics Engineering Design Institute, State Development & Investment Corporation, Beijing 100142, China; Beijing Key Laboratory of Algae Biomass, Beijing 100142, China.

Ultrafiltration membrane harvesting of Scenedesmus acuminatus was tested using alternative feed (AF) directions, i.e., bottom feed-top feed cycle and traditional bottom feed (BF). Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.007DOI Listing
February 2019
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Identification of enzymes from genus Trichoderma that can accelerate formation of ferulic acid and ethyl ferulate in collaboration with rice koji enzyme in sake mash.

J Biosci Bioeng 2019 Feb 19. Epub 2019 Feb 19.

Department of Biological Resource Sciences, Akita Prefectural University, Nakano Shimoshinjyo, Akita 010-0195, Japan. Electronic address:

The enzymes responsible for acceleration of ferulic acid and ethyl ferulate formation in sake mash were studied. Ferulic acid and ethyl ferulate are formed during the sake brewing process from feruloylated glucuronoarabinoxylan. Cellulase reagent from genus Trichoderma was used instead of rice koji, because rice koji for sake brewing produces extremely low levels of xylan-degrading enzymes. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.014DOI Listing
February 2019
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Biochemical characterization of a β-N-acetylhexosaminidase from Streptomyces alfalfae and its application in the production of N-acetyl-d-glucosamine.

J Biosci Bioeng 2019 Feb 16. Epub 2019 Feb 16.

College of Life Sciences, Hebei Agricultural University, Baoding 071000, P.R. China. Electronic address:

N-Acetyl-d-glucosamine (GlcNAc) is a valuable monosaccharide widely used in the medical, agricultural, biofuel, and food industries. Its efficient and environment-friendly production depends on the binary system of β-N-acetylhexosaminidase (HEX) and chitinase. In the present study, a HEX of glycoside hydrolasefamily 20 was identified in Streptomyces alfalfae ACCC40021, and was overexpressed in Escherichia coli. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.017DOI Listing
February 2019

Exploring the glyphosate-degrading characteristics of a newly isolated, highly adapted indigenous bacterial strain, Providencia rettgeri GDB 1.

J Biosci Bioeng 2019 Feb 16. Epub 2019 Feb 16.

Department of Chemical and Materials Engineering, National I-Lan University, I-Lan 26047, Taiwan. Electronic address:

This study explored the characteristics of a newly isolated glyphosate (GLYP)-degrading bacterium Providencia rettgeri GDB 1, for GLYP bioremediation. Due to the serial selection pressure of high GLYP concentrations for enriched isolation, this highly tolerant GLYP biodegrader shows very promising capabilities for GLYP removal (approximately 71.4% degradation efficiency) compared to previously reported strains. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183091
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http://dx.doi.org/10.1016/j.jbiosc.2019.01.012DOI Listing
February 2019
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Influence of graphene oxide and biochar on anaerobic degradation of petroleum hydrocarbons.

J Biosci Bioeng 2019 Feb 12. Epub 2019 Feb 12.

Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.

The anaerobic degradation of petroleum is an important process in natural environments. So far, few studies have considered the response of the microbial community to nanomaterials during this process. This study explored the potential effects of graphene oxide and biochar on the anaerobic degradation of petroleum hydrocarbons in long-term experiments. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.006DOI Listing
February 2019
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Gas chromatography-mass spectrometry metabolomics-based prediction of potato tuber sprouting during long-term storage.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

In order to supply potato (Solanum tuberosum L.) tubers for the processed food industry throughout the year, suppliers should provide consistent quality potatoes even after long-term storage. Despite being one of the most important foods, there is no simple way to control tuber quality and, in particular, controlling sprouting. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183073
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http://dx.doi.org/10.1016/j.jbiosc.2019.01.016DOI Listing
February 2019
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Cost analysis of oil cake-to-biodiesel production in packed-bed micro-flow reactors with immobilized lipases.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

Group Micro Flow Chemistry and Process Technology, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands; School of Chemical Engineering, The University of Adelaide, Adelaide, 5005 South Australia, Australia. Electronic address:

Biodiesel production depends to a great extent on the use of cheap raw materials, since biodiesel itself is a mass product, not a high-value product. New processing methods, such as micro-flow continuous processing combined with enzymatic catalysis, open doors to the latter. As reported here, the window of opportunity in enzyme-catalyzed biodiesel production is the conversion of waste cooking oil. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.004DOI Listing
February 2019

Time-course colony tracking analysis for evaluating induced pluripotent stem cell culture processes.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8602, Japan; Stem Cell Evaluation Technology Research Association (SCA), Hacho-bori, Chuou-ku, Tokyo 104-0032, Japan; Institute of Nano-Life-Systems, Institute of Innovation for Future Society, Nagoya University, Division of Micro-Nano Mechatronics, Furocho, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address:

Increasing the yield and maintaining a high quality of induced pluripotent stem cells (iPSCs) is necessary for manufacturing iPSCs at the industrial scale. However, because iPSCs are delicate, it is important to evaluate their quality during processing. To examine the status of cultured iPSCs non-invasively, morphology-based iPSC colony evaluation may be an efficient technology for cellular status monitoring and analysis. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.011DOI Listing
February 2019
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Biosynthesis of γ-aminobutyrate by engineered Lactobacillus brevis cells immobilized in gellan gum gel beads.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

γ-Aminobutyrate (GABA) is an important chemical in pharmaceutical field. The use of lactic acid bacteria as biocatalysts for the conversion of l-monosodium glutamate (MSG) into GABA opens interesting perspectives for the production of this functional compound. In this work, an engineered GABA high-producing strain Lactobacillus brevis GadA was constructed by overexpressing a C-terminally truncated GadA mutant, which is active in expanded pH range. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.010DOI Listing
February 2019

Electricity generation from sweet potato-shochu waste using microbial fuel cells.

J Biosci Bioeng 2019 Feb 5. Epub 2019 Feb 5.

Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address:

Electricity generation and treatment of sweet potato-shochu waste, acidic and organic-rich slurry, was examined using cassette-electrode microbial fuel cells (CE-MFCs). Among CE-MFCs with raw (73 g-chemical oxygen demand chromium COD/L) and different concentration of diluted sweet potato-shochu waste (0.5, 1, 5, 10, and 20 g-COD/L) without pH control, the maximum power density (1. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.015DOI Listing
February 2019
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Survival of Kluyveromyces marxianus with stigmasterol as subjected to freezing stress.

J Biosci Bioeng 2019 Feb 1. Epub 2019 Feb 1.

Department of Biological Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.

Kluyveromyces marxianus is an aerobic yeast and is interested to be applied in many industries. This research was aimed to study the effect of the sterol alternative to ergosterol on the freezing stress of K. marxianus UBU1-11, a thermotolerant yeast. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.003DOI Listing
February 2019

Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex.

J Biosci Bioeng 2019 Feb 1. Epub 2019 Feb 1.

Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan. Electronic address:

The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.003DOI Listing
February 2019

Peroxisomal Fba2p and Tal2p complementally function in the rearrangement pathway for xylulose 5-phosphate in the methylotrophic yeast Pichia pastoris.

J Biosci Bioeng 2019 Jan 30. Epub 2019 Jan 30.

Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address:

In this work, we analyzed several genes participating in the rearrangement pathway for xylulose 5-phosphate (Xu5P) in the methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii). P. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.008DOI Listing
January 2019
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Photobacterium sp. NNA4, an efficient hydroxylamine-transforming heterotrophic nitrifier/aerobic denitrifier.

J Biosci Bioeng 2019 Jan 29. Epub 2019 Jan 29.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

An efficient heterotrophic nitrifying/aerobic denitrifying strain, Photobacterium sp. NNA4 was isolated from a recirculating aquaculture system (RAS). NNA4 was capable of utilizing ammonia, nitrate or nitrite as sole N-source with maximal removal rates of 12. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.014DOI Listing
January 2019
2 Reads

Mutagenesis of Rhodobacter sphaeroides using atmospheric and room temperature plasma treatment for efficient production of coenzyme Q10.

J Biosci Bioeng 2019 Jan 29. Epub 2019 Jan 29.

College of Forestry, Beijing Forestry University, Beijing 100083, China.

Coenzyme Q10 (CoQ10) plays an important role in the human respiratory chain and is widely used as medicine and dietary supplement. To improve the fermentation efficiency of CoQ10, a modified version of atmospheric and room temperature plasma (ARTP) treatment was used to mutate Rhodobacter sphaeroides. Meanwhile, Vitamin K3, a structural analog of CoQ10, was used as an inhibitor for mutant selection. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.005DOI Listing
January 2019
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Identification and application of novel low pH-inducible promoters for lactic acid production in the tolerant yeast Candida glycerinogenes.

J Biosci Bioeng 2019 Jan 30. Epub 2019 Jan 30.

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address:

Bioproduction of organic acids under low pH condition without adding inducer and neutralizer is an economical craft to decrease downstream cost. Candida glycerinogenes had higher tolerances to low pH and lactic acid than Saccharomyces cerevisiae. QRT-PCR analysis showed that four of fifteen candidates functioned as potentially acid-inducible promoters in C. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.005DOI Listing
January 2019