4,602 results match your criteria Journal of Bioscience and Bioengineering [Journal]


Biochemical characterization of a β-N-acetylhexosaminidase from Streptomyces alfalfae and its application in the production of N-acetyl-d-glucosamine.

J Biosci Bioeng 2019 Feb 16. Epub 2019 Feb 16.

College of Life Sciences, Hebei Agricultural University, Baoding 071000, P.R. China. Electronic address:

N-Acetyl-d-glucosamine (GlcNAc) is a valuable monosaccharide widely used in the medical, agricultural, biofuel, and food industries. Its efficient and environment-friendly production depends on the binary system of β-N-acetylhexosaminidase (HEX) and chitinase. In the present study, a HEX of glycoside hydrolasefamily 20 was identified in Streptomyces alfalfae ACCC40021, and was overexpressed in Escherichia coli. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.017DOI Listing
February 2019

Exploring the glyphosate-degrading characteristics of a newly isolated, highly adapted indigenous bacterial strain, Providencia rettgeri GDB 1.

J Biosci Bioeng 2019 Feb 16. Epub 2019 Feb 16.

Department of Chemical and Materials Engineering, National I-Lan University, I-Lan 26047, Taiwan. Electronic address:

This study explored the characteristics of a newly isolated glyphosate (GLYP)-degrading bacterium Providencia rettgeri GDB 1, for GLYP bioremediation. Due to the serial selection pressure of high GLYP concentrations for enriched isolation, this highly tolerant GLYP biodegrader shows very promising capabilities for GLYP removal (approximately 71.4% degradation efficiency) compared to previously reported strains. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.012DOI Listing
February 2019

Influence of graphene oxide and biochar on anaerobic degradation of petroleum hydrocarbons.

J Biosci Bioeng 2019 Feb 12. Epub 2019 Feb 12.

Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.

The anaerobic degradation of petroleum is an important process in natural environments. So far, few studies have considered the response of the microbial community to nanomaterials during this process. This study explored the potential effects of graphene oxide and biochar on the anaerobic degradation of petroleum hydrocarbons in long-term experiments. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.006DOI Listing
February 2019
1 Read

Gas chromatography-mass spectrometry metabolomics-based prediction of potato tuber sprouting during long-term storage.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

In order to supply potato (Solanum tuberosum L.) tubers for the processed food industry throughout the year, suppliers should provide consistent quality potatoes even after long-term storage. Despite being one of the most important foods, there is no simple way to control tuber quality and, in particular, controlling sprouting. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183073
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http://dx.doi.org/10.1016/j.jbiosc.2019.01.016DOI Listing
February 2019
1 Read

Cost analysis of oil cake-to-biodiesel production in packed-bed micro-flow reactors with immobilized lipases.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

Group Micro Flow Chemistry and Process Technology, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands; School of Chemical Engineering, The University of Adelaide, Adelaide, 5005 South Australia, Australia. Electronic address:

Biodiesel production depends to a great extent on the use of cheap raw materials, since biodiesel itself is a mass product, not a high-value product. New processing methods, such as micro-flow continuous processing combined with enzymatic catalysis, open doors to the latter. As reported here, the window of opportunity in enzyme-catalyzed biodiesel production is the conversion of waste cooking oil. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.004DOI Listing
February 2019

Time-course colony tracking analysis for evaluating induced pluripotent stem cell culture processes.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8602, Japan; Stem Cell Evaluation Technology Research Association (SCA), Hacho-bori, Chuou-ku, Tokyo 104-0032, Japan; Institute of Nano-Life-Systems, Institute of Innovation for Future Society, Nagoya University, Division of Micro-Nano Mechatronics, Furocho, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address:

Increasing the yield and maintaining a high quality of induced pluripotent stem cells (iPSCs) is necessary for manufacturing iPSCs at the industrial scale. However, because iPSCs are delicate, it is important to evaluate their quality during processing. To examine the status of cultured iPSCs non-invasively, morphology-based iPSC colony evaluation may be an efficient technology for cellular status monitoring and analysis. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.011DOI Listing
February 2019
1 Read

Biosynthesis of γ-aminobutyrate by engineered Lactobacillus brevis cells immobilized in gellan gum gel beads.

J Biosci Bioeng 2019 Feb 7. Epub 2019 Feb 7.

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

γ-Aminobutyrate (GABA) is an important chemical in pharmaceutical field. The use of lactic acid bacteria as biocatalysts for the conversion of l-monosodium glutamate (MSG) into GABA opens interesting perspectives for the production of this functional compound. In this work, an engineered GABA high-producing strain Lactobacillus brevis GadA was constructed by overexpressing a C-terminally truncated GadA mutant, which is active in expanded pH range. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.010DOI Listing
February 2019

Electricity generation from sweet potato-shochu waste using microbial fuel cells.

J Biosci Bioeng 2019 Feb 5. Epub 2019 Feb 5.

Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address:

Electricity generation and treatment of sweet potato-shochu waste, acidic and organic-rich slurry, was examined using cassette-electrode microbial fuel cells (CE-MFCs). Among CE-MFCs with raw (73 g-chemical oxygen demand chromium COD/L) and different concentration of diluted sweet potato-shochu waste (0.5, 1, 5, 10, and 20 g-COD/L) without pH control, the maximum power density (1. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.015DOI Listing
February 2019

Survival of Kluyveromyces marxianus with stigmasterol as subjected to freezing stress.

J Biosci Bioeng 2019 Feb 1. Epub 2019 Feb 1.

Department of Biological Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.

Kluyveromyces marxianus is an aerobic yeast and is interested to be applied in many industries. This research was aimed to study the effect of the sterol alternative to ergosterol on the freezing stress of K. marxianus UBU1-11, a thermotolerant yeast. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.003DOI Listing
February 2019

Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex.

J Biosci Bioeng 2019 Feb 1. Epub 2019 Feb 1.

Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan. Electronic address:

The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.003DOI Listing
February 2019

Peroxisomal Fba2p and Tal2p complementally function in the rearrangement pathway for xylulose 5-phosphate in the methylotrophic yeast Pichia pastoris.

J Biosci Bioeng 2019 Jan 30. Epub 2019 Jan 30.

Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address:

In this work, we analyzed several genes participating in the rearrangement pathway for xylulose 5-phosphate (Xu5P) in the methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii). P. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.008DOI Listing
January 2019

Photobacterium sp. NNA4, an efficient hydroxylamine-transforming heterotrophic nitrifier/aerobic denitrifier.

J Biosci Bioeng 2019 Jan 29. Epub 2019 Jan 29.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

An efficient heterotrophic nitrifying/aerobic denitrifying strain, Photobacterium sp. NNA4 was isolated from a recirculating aquaculture system (RAS). NNA4 was capable of utilizing ammonia, nitrate or nitrite as sole N-source with maximal removal rates of 12. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.014DOI Listing
January 2019
1 Read

Mutagenesis of Rhodobacter sphaeroides using atmospheric and room temperature plasma treatment for efficient production of coenzyme Q10.

J Biosci Bioeng 2019 Jan 29. Epub 2019 Jan 29.

College of Forestry, Beijing Forestry University, Beijing 100083, China.

Coenzyme Q10 (CoQ10) plays an important role in the human respiratory chain and is widely used as medicine and dietary supplement. To improve the fermentation efficiency of CoQ10, a modified version of atmospheric and room temperature plasma (ARTP) treatment was used to mutate Rhodobacter sphaeroides. Meanwhile, Vitamin K3, a structural analog of CoQ10, was used as an inhibitor for mutant selection. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.005DOI Listing
January 2019

Identification and application of novel low pH-inducible promoters for lactic acid production in the tolerant yeast Candida glycerinogenes.

J Biosci Bioeng 2019 Jan 30. Epub 2019 Jan 30.

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address:

Bioproduction of organic acids under low pH condition without adding inducer and neutralizer is an economical craft to decrease downstream cost. Candida glycerinogenes had higher tolerances to low pH and lactic acid than Saccharomyces cerevisiae. QRT-PCR analysis showed that four of fifteen candidates functioned as potentially acid-inducible promoters in C. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.005DOI Listing
January 2019

Predictive selection and evaluation of appropriate functional peptides for intestinal delivery with a porous silica gel.

J Biosci Bioeng 2019 Jan 29. Epub 2019 Jan 29.

Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan; Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8601, Japan. Electronic address:

Bioactive peptides have a positive impact on body functions and conditions and may influence health. However, peptides are degraded by digestive enzymes, such as pepsin in the stomach when ingested orally. In order to solve this problem, we previously focused on porous silica gel and found that by using calcined silica gel, hydrophobic and negatively charged peptides could be efficiently delivered into the intestine, because peptides adsorbed on the cavity of the silica gel could be protected from enzymatic degradation. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2019.01.001DOI Listing
January 2019

Expression of alcohol oxidase gene from Ochrobactrum sp. AIU 033 in recombinant Escherichia coli through the twin-arginine translocation pathway.

J Biosci Bioeng 2019 Jan 28. Epub 2019 Jan 28.

Department of Biological Chemistry and Food Science, Iwate University, Ueda-3, Morioka 020-8550, Japan.

We cloned a set of genes encoding alcohol oxidase from Ochrobactrum sp. AIU 033 (OcAOD), which exhibits the appropriate substrate specificity for glyoxylic acid production from glycolic acid. The set of genes for OcAOD contained two open reading frames consisting of 555-bp (aodB) and 1572-bp (aodA) nucleotides, which encode the precursor for the β-subunit and α-subunit of OcAOD, respectively. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.012DOI Listing
January 2019

Overexpression and repression of the tyrosinase gene in Lentinula edodes using the pChG vector.

J Biosci Bioeng 2019 Jan 22. Epub 2019 Jan 22.

Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami-shi, Iwate 024-0003, Japan; Maruha Nichiro Holdings Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.

Tyrosinase is an industrially useful enzyme, however, it causes gill browning of Lentinula edodes fruiting bodies during preservation. In this study, we constructed two vectors, pChG-gTs and pChG-gTa, expressing sense and antisense tyrosinase gene of L. edodes, respectively, using promoters derived from the glyceraldehyde-3-phosphate dehydrogenase gene. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.013DOI Listing
January 2019

Date pits activated carbon for divalent lead ions removal.

J Biosci Bioeng 2019 Jan 21. Epub 2019 Jan 21.

Department of Chemical Engineering, The University of Nottingham-Malaysia Campus, Jalan Broga, 43500 Selangor Darul Ehsan, Malaysia. Electronic address:

Phosphoric acid impregnated activated carbon from date pits (DPAC) was prepared through single step activation. Prepared DPAC was studied for its structural, elemental, chemical, surface and crystal nature. Adsorption ability of the DPAC was assessed through divalent lead ions separation studies. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.011DOI Listing
January 2019

Mutated fabG gene encoding oxidoreductase enhances the cost-effective fermentation of jasmine rice vinegar in the adapted strain of Acetobacter pasteurianus SKU1108.

J Biosci Bioeng 2019 Jan 21. Epub 2019 Jan 21.

Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. Electronic address:

A low-nutrient adapted strain, Acetobacter pasteurianus G-40, was successfully obtained by repetitive cultivation of A. pasteurianus 7E-13 under selective pressure. The adapted strain could grow well and produce 3. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.006DOI Listing
January 2019

Ferrous chloride and ferrous sulfate improve the fungicidal efficacy of cold atmospheric argon plasma on melanized Aureobasidium pullulans.

J Biosci Bioeng 2019 Jan 21. Epub 2019 Jan 21.

Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki Hashiuecho 1, Kyoto 606-8585, Japan. Electronic address:

Since cold atmospheric pressure plasma (CAP) has not only bactericidal activity but also fungicidal activity without toxic residues and thermal damage, it is considered as an alternative method for sterilization of fungi on the surfaces of perishable foodstuffs and human bodies. Aureobasidium pullulans is a ubiquitous yeast-like fungus and called black yeast because it produces melanin, a dark biological pigment. It is well known that various melanized fungi show hyper-resistance to extreme stress conditions including high levels of radioactivity. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.008DOI Listing
January 2019
1 Read

Comparative biocontrol ability of chitinases from bacteria and recombinant chitinases from the thermophilic fungus Thermomyces lanuginosus.

J Biosci Bioeng 2019 Jan 19. Epub 2019 Jan 19.

Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa.

Microbial chitinases (EC 3.2.1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183037
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http://dx.doi.org/10.1016/j.jbiosc.2018.11.007DOI Listing
January 2019
5 Reads

Maintenance of an undifferentiated state of human-induced pluripotent stem cells through botulinum hemagglutinin-mediated regulation of cell behavior.

J Biosci Bioeng 2019 Jan 16. Epub 2019 Jan 16.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

Applications of human induced pluripotent stem cell (hiPSC) culture are impaired by problems with long term maintenance of pluripotency. In this study, we report that exposure to botulinum hemagglutinin (HA), an E-cadherin function-blocking agent, suppressed deviation from an undifferentiated state in hiPSC colonies. Time-lapse imaging of live cells revealed that cells in central regions of colonies moved slowly and underwent a morphological change to a cobblestone-like shape via interaction between contacting cells, forming dense, multiple layers. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.014DOI Listing
January 2019
2 Reads

Utility of a rotation/revolution-type agitator for chondrocyte isolation during preparation of engineered cartilage.

J Biosci Bioeng 2019 Jan 12. Epub 2019 Jan 12.

Division of Life Science and Engineering, School of Science and Engineering, Tokyo Denki University, Ishizaka, Hatoyama-cho, Hiki-gun, Saitama 350-0394, Japan. Electronic address:

During the manufacture of cell- and tissue-based products, such as engineered cartilage for autologous chondrocyte implantation, maximizing the number of cells isolated from donor tissue substantially improves the productivity of these products. The method used for agitating tissues with digestive fluid and enzymes can considerably affect both the quality and quantity of isolated cells. This study aimed to investigate the effectiveness of a rotation/revolution-type agitator for chondrocyte isolation following the enzymatic digestion of rat costal cartilage. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.010DOI Listing
January 2019

Acidogenic properties of carbohydrate-rich wasted potato and microbial community analysis: Effect of pH.

J Biosci Bioeng 2019 Jan 12. Epub 2019 Jan 12.

Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), China Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China.

Vegetable waste is one of the major organic solid residues available for sustainable biogas production. The aim of this study was to investigate the possibility and optimal controlling strategy for acidogenic fermentation of wasted potato (WP). Three leaching bed reactors (LBRs) were operated at various pH values (6. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.009DOI Listing
January 2019
4 Reads

Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks.

J Biosci Bioeng 2019 Jan 12. Epub 2019 Jan 12.

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation (STIN), Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.

The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183086
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http://dx.doi.org/10.1016/j.jbiosc.2018.12.002DOI Listing
January 2019
3 Reads

Anti-melanization effects and inhibitory kinetics of tyrosinase of bird's nest fern (Asplenium australasicum) frond extracts on melanoma and human skin.

J Biosci Bioeng 2019 Jan 10. Epub 2019 Jan 10.

Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd, Taichung 40227, Taiwan. Electronic address:

Some bioactive properties of p-coumaric acid and fucose-rich polysaccharide in skin health have been studied, including melanogenesis inhibition of the phenolic acid and growth inhibitory effects of the polysaccharide on melanoma. The dermatological benefits of bird's nest fern extracts (BNFE), containing both substantial fucose-rich polysaccharide and p-coumaric acid, like promoting collagen production and growth of fibroblast cell and further improving the elasticity and dryness of human skins have been demonstrated in our previous study. Besides, the anti-melanization effects of various BNFE on B16-F10 melanoma and human skin were first studied here. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.005DOI Listing
January 2019
6 Reads

Bio-ionic liquid pretreatment and ultrasound-promoted enzymatic hydrolysis of black soybean okara.

J Biosci Bioeng 2019 Jan 8. Epub 2019 Jan 8.

Department of Food Science, Fu Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang Dist., New Taipei City 24205, Taiwan, ROC. Electronic address:

The effective processing method to produce fermentable sugars and modify the microstructure of black soybean okara using bio-ionic liquid (bio-IL) pretreatment and ultrasound-promoted enzymatic hydrolysis was investigated. The morphology and structural characteristics of okara before and after bio-IL pretreatment and enzymatic hydrolysis under different ultrasonic frequencies were analyzed by field emission scanning electron microscope (FE-SEM), X-ray energy dispersive spectrometer (EDS), and Fourier transform infrared spectroscopy (FTIR). Without pretreatment, the production of total reducing sugar (TRS) under ultrasound (40 kHz/300 W) was 3. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.007DOI Listing
January 2019

Characterization and antioxidant activity of an acidic exopolysaccharide from Lactobacillus plantarum JLAU103.

J Biosci Bioeng 2018 Dec 29. Epub 2018 Dec 29.

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China. Electronic address:

An acidic exopolysaccharide (EPS) designated as EPS103 was isolated, purified and characterized from Lactobacillus plantarum JLAU103. EPS103 had a relatively lower molecular weight of 12.4 KDa and was consisted of arabinose, rhamnose, fucose, xylose, mannose, fructose, galactose, and glucose in an approximate molar ratio of 4. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.004DOI Listing
December 2018
2 Reads

Analyzing the relationship between the inorganic element profile of sake dilution water and dimethyl trisulfide formation using multi-element profiling.

J Biosci Bioeng 2018 Dec 28. Epub 2018 Dec 28.

Hakutsuru Sake Brewing Corporation, Ltd., 4-5-5 Sumiyoshiminami-machi, Higashinada-ku, Kobe, Hyogo 658-0041, Japan.

Dimethyl trisulfide (DMTS) is the main component of hineka, an off-flavor generated in sake during storage. Genshu, or undiluted sake, is usually diluted with water during warimizu, the process of adjusting the alcohol content of sake. In this study, we evaluated how the inorganic element composition of sake dilution water affects the DMTS-producing potential of the sake (DMTS-pp, determined as the DMTS concentration in sake stored at 70°C for 1 week after dilution) using inductively coupled plasma-mass spectrometry (ICP-MS). Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.013DOI Listing
December 2018

Cometabolic degradation of bisphenol A by pure culture of Ralstonia eutropha and metabolic pathway analysis.

J Biosci Bioeng 2018 Dec 28. Epub 2018 Dec 28.

Department of Pharmacology, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Bisphenol A (BPA) is a toxic compound emitting to the environment mainly by polycarbonate production facilities. In this research, BPA with the initial concentrations in the range of 1-40 mg l was degraded by Ralstonia eutropha. The bacteria were unable to use BPA as the sole carbon source. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.12.001DOI Listing
December 2018

Anaerobic glycerol-3-phosphate dehydrogenase complex from hyperthermophilic archaeon Thermococcus kodakarensis KOD1.

J Biosci Bioeng 2018 Dec 21. Epub 2018 Dec 21.

Department of Biomolecular Chemistry, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.

Glycerol-3-phosphate (G3P) is a key intermediate of glycerol metabolism and is oxidized to dihydroxyacetone phosphate aerobically or anaerobically by appropriate G3P dehydrogenases. A hyperthermophilic archaeon Thermococcus kodakarensis KOD1 has a novel operon consisting of three genes encoding an anaerobic G3P dehydrogenase (G3PDH), an NADH oxidase (NOX), and a molybdopterin oxidoreductase (MOX). Typically, the G3PDH gene (glpA) is included in an operon with genes encoding essential subunits of the G3PDH complex, glpB and glpC. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.012DOI Listing
December 2018

Secretory leakage of IgG1 aggregates from recombinant Chinese hamster ovary cells.

J Biosci Bioeng 2018 Dec 21. Epub 2018 Dec 21.

Graduate School of Engineering, Osaka University, U1E-801, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Aggregation of therapeutic antibodies is one of the most important issues to be resolved in manufacturing processes because of reduced efficacy and immunogenicity. Despite aggregation studies in vitro, little is known about the aggregation mechanism in cell culture processes. In this study, we investigated the process of aggregate formation of IgG1 antibodies during the culture of Chinese hamster ovary (CHO) cells to determine how aggregation occurs. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.015DOI Listing
December 2018

Physiological characterization of poly-β-hydroxybutyrate accumulation in the moderately thermophilic hydrogen-oxidizing bacterium Hydrogenophilus thermoluteolus TH-1.

J Biosci Bioeng 2018 Dec 19. Epub 2018 Dec 19.

Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address:

Hydrogenophilus thermoluteolus strain TH-1 is a thermophilic hydrogen-oxidizing microorganism that has the highest growth rate among autotrophs. Genomic analysis revealed that this strain comprises the complete gene set for poly-β-hydroxybutyrate (PHB) synthesis, i.e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183055
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http://dx.doi.org/10.1016/j.jbiosc.2018.11.011DOI Listing
December 2018
7 Reads

Lipolytic bacterial strains mediated transesterification of non-edible plant oils for generation of high quality biodiesel.

J Biosci Bioeng 2018 Dec 19. Epub 2018 Dec 19.

Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address:

Biodiesel is one of the best alternative to depleting fossil fuels for transport sector. However, biodiesel production via lipase mediated transesterification has limitation of high costing microbial enzymes. In order to overcome this limitation, a process of sequential treatment of oil industry wastewater using isolated lipolytic bacterial strains and biodiesel production from non-edible plant oils was studied. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.001DOI Listing
December 2018

High-cell density culture of poly(lactate-co-3-hydroxybutyrate)-producing Escherichia coli by using glucose/xylose-switching fed-batch jar fermentation.

J Biosci Bioeng 2018 Dec 17. Epub 2018 Dec 17.

Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kitaku, Sapporo 060-8628, Japan; CREST, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. Electronic address:

Poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] is produced in engineered Escherichia coli harboring the genes encoding an LA-polymerizing enzyme (LPE) and monomer-supplying enzymes. In this study, high cell-density fed-batch jar fermentation was developed using xylose and/or glucose as the carbon source. Fed-batch fermentation was initially performed with 20 g/L sugar during the batch phase for 24 h, and subsequent sugar feeding from 24 to 86 h. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.006DOI Listing
December 2018

In vitro and in vivo inhibition of Hass avocado polyphenol oxidase enzymatic browning by paeonol, β-cyclodextrin, and paeonol:β-cyclodextrin inclusion complex.

J Biosci Bioeng 2018 Dec 17. Epub 2018 Dec 17.

Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, IMIBIO-CONICET, Chacabuco 917, 5700 San Luis, Argentina.

Polyphenol oxidase (PPO) was extracted from Hass avocados and its physicochemical properties were analyzed. The optimum pH and temperature of the enzyme were pH 7.5 and 20°C. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.009DOI Listing
December 2018

Production of highly thermo-tolerant laccase from novel thermophilic bacterium Bacillus sp. PC-3 and its application in functionalization of chitosan film.

J Biosci Bioeng 2018 Dec 17. Epub 2018 Dec 17.

Department of Biotechnology, National Institute of Technology Raipur, Raipur, Chhattisgarh 492010, India. Electronic address:

In this study, a novel thermophilic bacterial strain was isolated from Tattapani hot spring located in the Chhattisgarh state of India. The laccase was produced via submerged fermentation and purified by ammonium sulfate precipitation and anion exchange chromatography up to 13.7 fold. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.008DOI Listing
December 2018

High-sensitive liquid chromatography-tandem mass spectrometry-based chiral metabolic profiling focusing on amino acids and related metabolites.

J Biosci Bioeng 2018 Dec 15. Epub 2018 Dec 15.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

Metabolomics has been an evolving science with a wide range of applications in various fields. However, previous studies have rarely focused on metabolite chirality. In this study, to achieve metabolic profiling of chiral amino acids and related metabolites, we developed a high-throughput method using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.10.003DOI Listing
December 2018

Exoelectrogenic response of Pichia fermentans influenced by mediator and reactor design.

J Biosci Bioeng 2018 Dec 10. Epub 2018 Dec 10.

Department of Biosciences, Manipal University, Jaipur 303007, Rajasthan, India. Electronic address:

Microbial fuel cell is one of the most convenient and cost-effective technology for producing the clean energy. This study explores the exoelectrogenic behavior of Pichia fermentans in a microbial fuel cell. Two different reactor designs (double- and single-chambered) were tested in the presence and in the absence of a mediator (methylene blue). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183074
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http://dx.doi.org/10.1016/j.jbiosc.2018.11.004DOI Listing
December 2018
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Removal of selenite from artificial wastewater with high salinity by activated sludge in aerobic sequencing batch reactors.

J Biosci Bioeng 2018 Nov 28. Epub 2018 Nov 28.

Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

Selenite (Se(IV)) in artificial wastewater under high-salinity conditions of 70 g/L was treated by using sequencing batch reactors (SBRs). Activated sludge derived from a municipal wastewater treatment plant was acclimated to high salinity and was subsequently subjected to Se(IV) treatment. Two SBRs with different operating conditions, SBR-A and -B, were tested; the former was fed with influent containing a higher concentration of Se(IV) at 100 mg Se/L, whereas the Se(IV) concentration of influent for the latter was increased stepwise at 20-100 mg Se/L. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.002DOI Listing
November 2018

In vitro synthesis of the human calcium transporter Letm1 within cell-sized liposomes and investigation of its lipid dependency.

J Biosci Bioeng 2018 Nov 28. Epub 2018 Nov 28.

Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

The human mitochondrion-derived calcium transporter Letm1 was synthesized by reconstituted in vitro transcription-translation (IVTT) in cell-sized liposomes and the dependency of Letm1 on phospholipid composition was investigated. Components for IVTT were encapsulated into cell-sized vesicles together with the DNA encoding Letm1, thereby preparing proteoliposomes. The synthesis of Letm1 and pH-dependent calcium transport activity were confirmed by flow cytometry. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.11.003DOI Listing
November 2018

Hematopoiesis-supportive function of growth-arrested human adipose-tissue stromal cells under physiological hypoxia.

J Biosci Bioeng 2018 Nov 29. Epub 2018 Nov 29.

Cell Physiology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye shosse, 76a, 123007 Moscow, Russia; Faculty of Basic Medicine, Moscow State University, Lomonosovsky Prospekt, 31-5, 117192 Moscow, Russia.

Ex vivo expansion of hematopoietic progenitors is considered as an attractive tool to increase the number of stem and progenitor cells (HSPCs) for cell therapy. The efficacy of ex vivo expansion is strongly depends on the feeder cell activity to mimic hematopoietic microenvironment. Here we demonstrated, that combination of mitomycin C-induced growth arrest and tissue-related O (physiological hypoxia) modulated stromal capacity of adipose tissue derived stromal cells (ASCs). Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.10.017DOI Listing
November 2018
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Nanaomycin I and J: New nanaomycins generated by mycothiol-mediated compounds from "Streptomyces rosa subsp. notoensis" OS-3966.

J Biosci Bioeng 2018 Nov 29. Epub 2018 Nov 29.

Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan; Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan. Electronic address:

Two new nanaomycin analogs, nanaomycin I and J, were isolated from a cultured broth of an actinomycete strain, "Streptomyces rosa subsp. notoensis" OS-3966. In our previous study, we have confirmed the occurrence of nanaomycin I (m/z = 482 [M + H]) that lacks a pseudo-disaccharide from the mycothiol of nanaomycin H under same culture condition. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.10.013DOI Listing
November 2018
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Elucidation of human induced pluripotent stem cell behaviors in colonies based on a kinetic model.

J Biosci Bioeng 2018 Nov 27. Epub 2018 Nov 27.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

Maintaining the homogeneity of a stem cell population is one of the challenges in bioprocessing prior to therapeutic applications of stem cells. Concerning human induced pluripotent stem cell (hiPSC) colonies cultured on feeder cells, cells at the peripheral region of the colony were found to have a higher average movement rate than cells at the central region of the colony. This spatial difference in average movement rate might lead to spatial heterogeneity of cell fate decision in the colony. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.10.016DOI Listing
November 2018

Performance and dynamic characteristics of microbial communities in multi-stage anaerobic reactors treating gibberellin wastewater.

J Biosci Bioeng 2019 Mar 1;127(3):318-325. Epub 2018 Dec 1.

Beijing Engineering Research Center of Environmental Material for Water Purification, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:

To treat gibberellin (GA) wastewater, a full-scale, multi-stage combined contact process was developed. This whole process employs three anaerobic reactors followed by micro-aerobic, anoxic/aeration and biological oxidation treatment. Pollutant removal results showed that the combined process could remove more than 98% of the chemical oxygen demand (COD), NHN, and SO pollutants because of the different microbial communities in each reactor. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.05.017DOI Listing
March 2019
2 Reads

Cloning, expression, and characterization of novel GH5 endoglucanases from Thermobifida alba AHK119.

J Biosci Bioeng 2018 Dec 1. Epub 2018 Dec 1.

Center for Fiber and Textile Science, Kyoto Institute of Technology, Kyoto 606-8181, Japan.

Thermobifida alba AHK119 exhibits sufficient filter paper-degradation activity in its culture supernatant. AHK119-bMs (1365 bp) and AHK119-E5 (1425 bp), which encode novel GH5 family endoglucanases, were cloned from the genomic DNA of T. alba AHK119. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13891723183019
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http://dx.doi.org/10.1016/j.jbiosc.2018.10.015DOI Listing
December 2018
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Enhancement of the cultivation process conditions of mixed culture methanotrophic Proteobacteria phylum enriched from waste activated sludge as the first step for value added recovery process.

J Biosci Bioeng 2018 Nov 27. Epub 2018 Nov 27.

Department of Civil Engineering, Lassonde School of Engineering, York University, Toronto, Ontario, M3J 1P3, Canada. Electronic address:

Methanotrophs are of great interest due to their distinguish ability of recovering value-added commodities such as methanol and lipids while mitigating methane. The enhancement of methanotrophs cultivation process conditions is a pivotal step to develop a feasible methanotrophic bioreactor. In this study, multiple batch tests have been performed to evaluate the aqueous growth medium elements including nitrogen, copper, and biomass density and the gaseous headspace composition influence on methanotrophs activity and the associated microbial community. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.10.018DOI Listing
November 2018

Metabolome analysis revealed the knockout of glyoxylate shunt as an effective strategy for improvement of 1-butanol production in transgenic Escherichia coli.

J Biosci Bioeng 2019 Mar 25;127(3):301-308. Epub 2018 Oct 25.

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

High 1-butanol titer has been achieved in a transgenic Escherichia coli strain JCL299FT with a heterologous 1-butanol pathway by deleting competing pathways, balancing of cofactor and resolving free CoA imbalance. However, further improvement of 1-butanol production is still possible in the highest producing strain JCL299FT as indicated by the accumulation of acetate, a major undesired by-product during bio-production by microorganisms that competes with 1-butanol production for the available acetyl-CoA and inhibits protein synthesis resulting in poor growth. In this study, liquid chromatography/tandem mass spectrometry (LC/MS/MS)-based metabolome analysis was performed to identify new rate limiting steps in the 1-butanol production pathway of E. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.08.013DOI Listing
March 2019
1 Read

Bio-electrodegradation of 2,4,6-Trichlorophenol by mixed microbial culture in dual chambered microbial fuel cells.

J Biosci Bioeng 2019 Mar 25;127(3):353-359. Epub 2018 Oct 25.

Environmental Research Laboratory, Department of Chemistry, Aligarh Muslim University, Aligarh 202 002, UP, India. Electronic address:

2,4,6-Trichlorophenol (TCP) was bioelectrochemically treated in anodic and cathodic compartments of two identical dual chambered microbial fuel cells MFC-A and MFC-B under anaerobic and aerobic conditions, respectively, and energy was recovered in the form of electricity. It was observed that MFC-B with bio-cathodic treatment of TCP outcompeted the MFC-A with bio-anodic treatment. The maximum power density for MFC-A with bio-anode was found to be 446. Read More

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http://dx.doi.org/10.1016/j.jbiosc.2018.08.012DOI Listing