6,211 results match your criteria Journal of Biomedical Optics [Journal]


Excitation parameters optimized for coherent anti-Stokes Raman scattering imaging of myelinated tissue.

J Biomed Opt 2019 Apr;24(4):1-8

University of Calgary, Hotchkiss Brain Institute, Cumming School of Medicine, Department of Clinical, Canada.

Coherent anti-Stokes Raman scattering (CARS) generates a strong label-free signal in the long wavenumber C─H stretching region. Lipid-rich myelinated tissues, such as brain and spinal cord, would appear to be ideal subjects for imaging with CARS laser-scanning microscopy. However, the highly ordered, biochemically complex, and highly scattering nature of such tissues complicate the use of the technique. Read More

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http://dx.doi.org/10.1117/1.JBO.24.4.046502DOI Listing

Phantom-based evaluation of near-infrared intracranial hematoma detector performance.

J Biomed Opt 2019 Apr;24(4):1-10

U.S. Food and Drug Administration, Center for Devices and Radiological Health, Silver Spring, Maryla, United States.

Near-infrared spectroscopy (NIRS) is emerging as a rapid, low-cost approach for point-of-care triage of hematomas resulting from traumatic brain injury. However, there remains a lack of standardized test methods for benchtop performance assessment of these devices and incomplete understanding of relevant light-tissue interactions. We propose a phantom-based test method for systems operating near the 800-nm oxy-/deoxy-hemoglobin isosbestic point and implement it to evaluate a clinical system. Read More

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http://dx.doi.org/10.1117/1.JBO.24.4.045001DOI Listing
April 2019
2 Reads

Unraveling the molecular nature of melanin changes in metastatic cancer.

J Biomed Opt 2019 Apr;24(5):1-13

Duke University, Department of Chemistry, Durham, North Carolina, United States.

More people die from melanoma after a stage I diagnosis than after a stage IV diagnosis, because the tools available to clinicians do not readily identify which early-stage cancers will be aggressive. Near-infrared pump-probe microscopy detects fundamental differences in melanin structure between benign human moles and melanoma and also correlates with metastatic potential. However, the biological mechanisms of these changes have been difficult to quantify, as many different mechanisms can contribute to the pump-probe signal. Read More

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http://dx.doi.org/10.1117/1.JBO.24.5.051414DOI Listing
April 2019
1 Read

Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues.

J Biomed Opt 2019 Apr;24(4):1-6

Stanford University, Edward L. Ginzton Laboratory, Stanford, California, United States.

We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude. Read More

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http://dx.doi.org/10.1117/1.JBO.24.4.046501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454294PMC
April 2019
1 Read

Photoacoustic imaging in the second near-infrared window: a review.

J Biomed Opt 2019 Apr;24(4):1-20

Nanyang Technological University, School of Chemical and Biomedical Engineering, Singapore.

Photoacoustic (PA) imaging is an emerging medical imaging modality that combines optical excitation and ultrasound detection. Because ultrasound scatters much less than light in biological tissues, PA generates high-resolution images at centimeters depth. In recent years, wavelengths in the second near-infrared (NIR-II) window (1000 to 1700 nm) have been increasingly explored due to its potential for preclinical and clinical applications. Read More

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http://dx.doi.org/10.1117/1.JBO.24.4.040901DOI Listing
April 2019
2 Reads
2.859 Impact Factor

Transducer-matched multipulse excitation for signal-to-noise ratio improvement in diode laser-based photoacoustic systems.

J Biomed Opt 2019 Apr;24(4):1-8

Ruhr University Bochum, Photonics and Terahertz Technology, Faculty of Electrical Engineering and In, Germany.

We analyze transducer-matched multipulse excitation as a method for improving of the signal-to-noise ratio (SNR) for diode laser-based photoacoustic systems. We discuss the principle of the technique, its advantages, and potential drawbacks and perform measurements to analyze the obtainable SNR increase. We show in experiment and computationally that a lower boundary estimate of 1. Read More

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http://dx.doi.org/10.1117/1.JBO.24.4.046001DOI Listing

Single snapshot of optical properties image quality improvement using anisotropic two-dimensional windows filtering.

J Biomed Opt 2019 Mar;24(7):1-21

University of Strasbourg, ICube Laboratory, Illkirch, France.

Imaging methods permitting real-time, wide-field, and quantitative optical mapping of biological tissue properties offer an unprecedented range of applications for clinical use. Following the development of spatial frequency domain imaging, we introduce a real-time demodulation method called single snapshot of optical properties (SSOPs). However, since this method uses only a single image to generate absorption and reduced scattering maps, it was limited by a degraded image quality resulting in artifacts that diminished its potential for clinical use. Read More

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http://dx.doi.org/10.1117/1.JBO.24.7.071611DOI Listing

Perspective review on applications of optics in cerebral endovascular neurosurgery.

J Biomed Opt 2019 Mar;24(3):1-7

University of Toronto, Division of Neurosurgery, Department of Surgery, Toronto, Ontario, Canada.

Cerebral endovascular neurosurgery has transformed the way we manage cerebrovascular disease. Several landmark trials have demonstrated the effectiveness of endovascular techniques leading to continued technological development and applications for various diseases. The utilization of optical technologies and devices is already underway in the field of endovascular neurosurgery. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.030601DOI Listing
March 2019
1 Read

Thulium fiber laser ablation of kidney stones using an automated, vibrating fiber.

J Biomed Opt 2019 Mar;24(3):1-10

University of North Carolina at Charlotte, Department of Physics and Optical Science, Charlotte, Nor, United States.

Our preliminary study investigates an automated, vibrating fiber optic tip for dusting of kidney stones during thulium fiber laser (TFL) lithotripsy. A (0.75-mm diameter and 5-mm length) magnetic bead was attached to the fiber jacket, centered 2 cm from distal fiber tip. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.038001DOI Listing

Concentration of FAD as a marker for cervical precancer detection.

J Biomed Opt 2019 Mar;24(3):1-7

Indian Institute of Technology Kanpur, Department of Physics, Kanpur, Uttar Pradesh, India.

We report the ex vivo results of an in-house fabricated portable device based on polarized fluorescence measurements in the clinical environment. This device measures the polarized fluorescence and elastic scattering spectra with 405-nm laser and white light sources, respectively. The dominating fluorophore with 405-nm excitation is flavin adenine dinucleotide (FAD) with a fluorescence peak around 510 nm. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035008DOI Listing

Connectivity properties in the prefrontal cortex during working memory: a near-infrared spectroscopy study.

J Biomed Opt 2019 Mar;24(5):1-7

Britton Chance Ctr. for Biomedical Photonics, Huazhong Univ. of Science and Technology, China.

Working memory (WM) plays a crucial role in human brain functions. The application of brain connectivity analysis helps to understand the brain network properties in WM. Combination of functional and effective connectivity can provide new insights for exploring network attributes. Read More

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http://dx.doi.org/10.1117/1.JBO.24.5.051410DOI Listing
March 2019
2.859 Impact Factor

Optical biopsy of head and neck cancer using hyperspectral imaging and convolutional neural networks.

J Biomed Opt 2019 Mar;24(3):1-9

University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States.

For patients undergoing surgical cancer resection of squamous cell carcinoma (SCCa), cancer-free surgical margins are essential for good prognosis. We developed a method to use hyperspectral imaging (HSI), a noncontact optical imaging modality, and convolutional neural networks (CNNs) to perform an optical biopsy of ex-vivo, surgical gross-tissue specimens, collected from 21 patients undergoing surgical cancer resection. Using a cross-validation paradigm with data from different patients, the CNN can distinguish SCCa from normal aerodigestive tract tissues with an area under the receiver operator curve (AUC) of 0. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036007DOI Listing
March 2019
1 Read

Hyperspectral near-infrared spectroscopy assessment of the brain during hypoperfusion.

J Biomed Opt 2019 Mar;24(3):1-6

St. Michael's Hospital, Li Ka Shing Knowledge Institute, Toronto, Ontario, Canada.

Two-thirds of out-of-hospital cardiac arrest patients, who survive to hospital admission, die in the hospital from neurological injuries related to cerebral hypoperfusion. Therefore, noninvasive real-time monitoring of the cerebral oxygen metabolism in cardiac arrest patients is extremely important. Hyperspectral near-infrared spectroscopy (hNIRS) is a noninvasive technique that measures concentrations of the key chromophores in the brain, such as oxygenated hemoglobin, deoxygenated hemoglobin, and cytochrome C oxidase (CCO), an intracellular marker of oxygen consumption. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035007DOI Listing
March 2019
2 Reads

Slope-based segmentation of articular cartilage using polarization-sensitive optical coherence tomography phase retardation image.

J Biomed Opt 2019 Mar;24(3):1-14

University of British Columbia, Department of Electrical and Computer Engineering, Vancouver, Britis, Canada.

A segmentation method based on phase retardation measurements from polarization-sensitive optical coherence tomography (PS-OCT) is developed to differentiate the structural zones of articular cartilage. The organization of collagen matrix in articular cartilage varies over the different structural zones, generating different tissue birefringence. Analyzing the slope of the accumulated phase retardation at different depths can detect the variation in tissue birefringence and be used to segment the structural zones. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036006DOI Listing
March 2019
2 Reads

Validation of combined Monte Carlo and photokinetic simulations for the outcome correlation analysis of benzoporphyrin derivative-mediated photodynamic therapy on mice.

J Biomed Opt 2019 Mar;24(3):1-9

University of Pennsylvania, Department of Radiation Oncology, Philadelphia, Pennsylvania, United States.

We compare previously reported benzoporphyrin derivative (BPD)-mediated photodynamic therapy (PDT) results for reactive singlet oxygen concentration (also called singlet oxygen dose) on mice with simulations using a computational device, Dosie™, that calculates light transport and photokinetics for PDT in near real-time. The two sets of results are consistent and validate the use of the device in PDT treatment planning to predict BPD-mediated PDT outcomes in mice animal studies based on singlet oxygen dose, which showed a much better correlation with the cure index than the conventional light dose. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416474PMC

Fiber-optic plasmonic probe with nanogap-rich Au nanoislands for on-site surface-enhanced Raman spectroscopy using repeated solid-state dewetting.

J Biomed Opt 2019 Mar;24(3):1-6

Korea Advanced Institute of Science and Technology (KAIST), Department of Bio and Brain Engineering,, Republic of Korea.

We report a fiber-optic plasmonic probe with nanogap-rich gold nanoislands for on-site surface-enhanced Raman spectroscopy (SERS). The plasmonic probe features nanogap-rich Au nanoislands on the top surface of a single multimode fiber. Au nanoislands were monolithically fabricated using repeated solid-state dewetting of thermally evaporated Au thin film. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.037001DOI Listing
March 2019
2 Reads

Real-time, wide-field, and quantitative oxygenation imaging using spatiotemporal modulation of light.

J Biomed Opt 2019 Mar;24(7):1-7

University of Strasbourg, ICube Laboratory, Strasbourg, France.

Quantitative diffuse optical imaging has the potential to provide valuable functional information about tissue status, such as oxygen saturation or blood content to healthcare practitioners in real time. However, significant technical challenges have so far prevented such tools from being deployed in the clinic. Toward achieving this goal, prior research introduced methods based on spatial frequency domain imaging (SFDI) that allow real-time (within milliseconds) wide-field imaging of optical properties but at a single wavelength. Read More

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http://dx.doi.org/10.1117/1.JBO.24.7.071610DOI Listing

Normalized field autocorrelation function-based optical coherence tomography three-dimensional angiography.

J Biomed Opt 2019 Mar;24(3):1-8

Boston University, Neurophotonics Center, Department of Biomedical Engineering, Boston, Massachusett, United States.

Optical coherence tomography angiography (OCTA) has been widely used for en face visualization of the microvasculature, but is challenged for real three-dimensional (3-D) topologic imaging due to the "tail" artifacts that appear below large vessels. Further, OCTA is generally incapable of differentiating descending arterioles from ascending venules. We introduce a normalized field autocorrelation function-based OCTA (g1-OCTA), which minimizes the tail artifacts and is capable of distinguishing penetrating arterioles from venules in the 3-D image. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414735PMC

Optical coherence elastography of cold cataract in porcine lens.

J Biomed Opt 2019 Mar;24(3):1-7

Univ. of Houston, United States.

Cataract is one of the most prevalent causes of blindness around the world. Understanding the mechanisms of cataract development and progression is important for clinical diagnosis and treatment. Cold cataract has proven to be a robust model for cataract formation that can be easily controlled in the laboratory. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444576PMC
March 2019
5 Reads

Large field-of-view phase and fluorescence mesoscope with microscopic resolution.

J Biomed Opt 2019 Mar;24(3):1-9

Université Grenoble Alpes, CEA, LETI, Grenoble, France.

Phase and fluorescence are complementary contrasts that are commonly used in biology. However, the coupling of these two modalities is traditionally limited to high magnification and complex imaging systems. For statistical studies of biological populations, a large field-of-view is required. Read More

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https://www.spiedigitallibrary.org/journals/journal-of-biome
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http://dx.doi.org/10.1117/1.JBO.24.3.036501DOI Listing
March 2019
6 Reads

Optical phantoms for biomedical polarimetry: a review.

J Biomed Opt 2019 Mar;24(3):1-12

Florida International University, Department of Biomedical Engineering, Miami, Florida, United States.

Calibration, quantification, and standardization of the polarimetric instrumentation, as well as interpretation and understanding of the obtained data, require the development and use of well-calibrated phantoms and standards. We reviewed the status of tissue phantoms for a variety of applications in polarimetry; more than 500 papers are considered. We divided the phantoms into five groups according to their origin (biological/nonbiological) and fundamental polarimetric properties of retardation, depolarization, and diattenuation. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.030901DOI Listing

Characterizing short-wave infrared fluorescence of conventional near-infrared fluorophores.

J Biomed Opt 2019 Mar;24(3):1-5

Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States.

The observed behavior of short-wave infrared (SWIR) light in tissue, characterized by relatively low scatter and subdiffuse photon transport, has generated considerable interest for the potential of SWIR imaging to produce high-resolution, subsurface images of fluorescence activity in vivo. These properties have important implications for fluorescence-guided surgery and preclinical biomedical research. Until recently, translational efforts have been impeded by the conventional understanding that fluorescence molecular imaging in the SWIR regime requires custom molecular probes that do not yet have proven safety profiles in humans. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408334PMC
March 2019
1 Read

Multispectral imaging of cortical vascular and hemodynamic responses to a shock wave: observation of spreading depolarization and oxygen supply-demand mismatch.

J Biomed Opt 2019 Mar;24(3):1-17

Tokyo University of Agriculture and Technology, Graduate School of Bio-Applications and Systems Engi, Japan.

Blast-induced traumatic brain injury has been a recent major concern in neurotraumatology. However, its pathophysiology and mechanism are not understood partly due to insufficient information on the brain pathophysiology during/immediately after shock wave exposure. We transcranially applied a laser-induced shock wave (LISW, ∼19  Pa  ·  s) to the left frontal region in a rat and performed multispectral imaging of the ipsilateral cortex through a cranial window (n  =  4). Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035005DOI Listing

Parametric standing wave generation of a shallow reflection plane in a nonrigid sample for use in a noninvasive blood glucose monitor.

J Biomed Opt 2019 Mar;24(3):1-7

Kagawa University, Faculty of Engineering, Takamatsu-City, Kagawa, Japan.

When monitoring a moist sample using mid-infrared spectroscopy, its thickness must be <100  μm to avoid light absorption from the water. Therefore, we propose an ultrasonic-assisted mid-infrared spectroscopic imaging method that can generate a reflection plane at a depth of 100  μm from the surface of the sample by creating an ultrasonic standing wave. A frequency of 10 MHz is required to obtain an optical path length of 100  μm in biological samples. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036003DOI Listing
March 2019
1 Read

Measurement of the refractive index of whole blood and its components for a continuous spectral region.

J Biomed Opt 2019 Mar;24(3):1-5

Nankai University, School of Physics and TEDA Applied Physics, Key Laboratory of Weak-Light Nonlinea, China.

The refractive index of blood is a key biophysical parameter, which can reflect the physiological state. We measured the refractive index of whole blood and other components, such as serum, plasma, and hemoglobin, based on internal reflection by using a homemade apparatus in the spectral range of 400 to 750 nm. In addition to the hemoglobin solution, which has a Soret band about 420 nm and two Q-bands between 500 and 600 nm, the measurements of other samples are the normal dispersion curve. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403469PMC

Comparison of phosphorescent agents for noninvasive sensing of tumor oxygenation via Cherenkov-excited luminescence imaging.

J Biomed Opt 2019 Mar;24(3):1-8

Thayer School of Engineering at Dartmouth, United States.

Cherenkov emission generated in tissue during radiotherapy can be harnessed for the imaging biochemistry of tissue microenvironments. Cherenkov-excited luminescence scanned imaging (CELSI) provides a way to optically and noninvasively map oxygen-related signals, which is known to correlate to outcomes in radiotherapy. Four candidate phosphorescent reagents PtG4, MM2, Ir(btb)2  (  acac  )  , and MitoID were studied for oxygen sensing, testing in a progressive series of (a) in solution, (b) in vitro, and (c) in subcutaneous tumors. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397946PMC
March 2019
1 Read

Optimized depth-resolved estimation to measure optical attenuation coefficients from optical coherence tomography and its application in cerebral damage determination.

J Biomed Opt 2019 Mar;24(3):1-11

Northeastern Univ. at Qinhuangdao, China.

The optical attenuation coefficient (OAC) reflects the optical properties of various tissues or tissues of the same type under different physiological conditions. Quantitative measurement of OAC from optical coherence tomography (OCT) signals can provide additional information and can increase the potential for OCT applications. We present an optimized depth-resolved estimation (ODRE) method that derives a precise mapping between the measured OCT signal and the OAC. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035002DOI Listing
March 2019
8 Reads
2.859 Impact Factor

Near-infrared imaging of demineralization on the occlusal surfaces of teeth without the interference of stains.

J Biomed Opt 2019 Mar;24(3):1-8

Univ. of California, San Francisco, United States.

Most new caries lesions are found in the pits and fissures of the occlusal surface. Radiographs have extremely low sensitivity for early occlusal decay, and by the time the lesion is severe enough to appear on a radiograph, it typically has penetrated well into the dentin and surgical intervention is required. The occlusal surfaces are often heavily stained, and visual and tactile detection have poor sensitivity and specificity. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.036002DOI Listing
March 2019
2 Reads

Dynamic visualization of the whole process of cytotoxic T lymphocytes killing B16 tumor cells in vitro.

J Biomed Opt 2019 Mar;24(5):1-7

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazho, China.

Cytotoxic T lymphocytes (CTLs) play a key role in adoptive cell therapy (ACT) by destroying tumor cells. Although some mechanisms of CTLs killing tumor cells have already been revealed, the precise dynamic information of CTLs' interaction with tumor cells is still not known. Here, we used confocal microscopy to visualize the whole process of how CTLs kill tumor cells in vitro. Read More

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http://dx.doi.org/10.1117/1.JBO.24.5.051413DOI Listing
March 2019
2.859 Impact Factor

Optical catapulting of microspheres in mucus models-toward overcoming the mucus biobarrier.

J Biomed Opt 2019 Mar;24(3):1-9

Technical University of Denmark, DTU Fotonik, Department of Photonics Engineering, Kongens Lyngby, Denmark.

The generalized phase contrast method is employed as an efficient "phase-only" laser beam-shaping technique in an optical setup built for catapulting microspheres through simple mucus models. The influence of the laser power and mucin concentration on the motion of the microspheres is investigated in terms of instant and average velocities on a 250-μm trajectory, corresponding to the mucus thickness in the human gastrointestinal tract. Increasing the laser power leads to higher velocities in all the tested samples, while increasing the mucin concentration leads to significant velocity decrease for similar laser input power. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.035001DOI Listing
March 2019
2 Reads

Blue diode laser versus traditional infrared diode laser and quantic molecular resonance scalpel: clinical and histological findings after excisional biopsy of benign oral lesions (Erratum).

J Biomed Opt 2019 Feb;24(2)

Division of Oral Medicine and Pathology, Dental Clinic, Ospedale Maggiore, Trieste, Italy.

This erratum corrects the spelling of an author's name. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.029803DOI Listing
February 2019
2 Reads
2.859 Impact Factor

Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields.

J Biomed Opt 2019 Feb;24(5):1-11

Cardiff University, School of Engineering, Cardiff, Wales, United Kingdom.

Biological systems with intrinsic luminescent properties serve as powerful and noninvasive bioreporters for real-time and label-free monitoring of cell physiology. This study employs the bioluminescent marine bacterium Vibrio fischeri to investigate the effects of separated microwave electric (E) and magnetic (H) fields. Using a cylindrical TM010 mode aluminum resonant cavity, designed to spatially separate E and H fields of a pulsed microwave (2. Read More

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http://dx.doi.org/10.1117/1.JBO.24.5.051412DOI Listing
February 2019
1 Read

Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows.

J Biomed Opt 2019 Feb;24(2):1-15

University of Milano-Bicocca, Department of Physics, Milan, Italy.

Mapping flows in vivo is essential for the investigation of cardiovascular pathologies in animal models. The limitation of optical-based methods, such as space-time cross correlation, is the scattering of light by the connective and fat components and the direct wave front distortion by large inhomogeneities in the tissue. Nonlinear excitation of the sample fluorescence helps us by reducing light scattering in excitation. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.025004DOI Listing
February 2019
5 Reads

Design, fabrication, and feasibility analysis of a colorimetric detection system with a smartphone for self-monitoring blood glucose.

J Biomed Opt 2019 Feb;24(2):1-7

iXensor Co. Ltd., Taipei, Taiwan.

Maintaining appropriate insulin levels is very important for diabetes patients. Effective monitoring of blood glucose can aid in maintaining the body's insulin level, and thus reduce disease severities, secondary complications, and related mortalities. However, existing blood glucose measurement devices are inconvenient to carry and involve complex procedures, reducing the willingness of diabetes patients to regularly measure blood glucose. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.027002DOI Listing
February 2019
4 Reads

Development of an integrated multimodal optical imaging system with real-time image analysis for the evaluation of oral premalignant lesions.

J Biomed Opt 2019 Feb;24(2):1-10

Rice University, Department of Bioengineering, Houston, Texas, United States.

Oral premalignant lesions (OPLs), such as leukoplakia, are at risk of malignant transformation to oral cancer. Clinicians can elect to biopsy OPLs and assess them for dysplasia, a marker of increased risk. However, it is challenging to decide which OPLs need a biopsy and to select a biopsy site. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.025003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383051PMC
February 2019
1 Read

Dual-grid mesh-based Monte Carlo algorithm for efficient photon transport simulations in complex three-dimensional media.

J Biomed Opt 2019 Feb;24(2):1-4

Northeastern Univ., United States.

The mesh-based Monte Carlo (MMC) method is an efficient algorithm to model light propagation inside tissues with complex boundaries, but choosing appropriate mesh density can be challenging. A fine mesh improves the spatial resolution of the output but requires more computation. We propose an improved MMC-dual-grid mesh-based Monte Carlo (DMMC)-to accelerate photon simulations using a coarsely tessellated tetrahedral mesh for ray-tracing computation and an independent voxelated grid for output data storage. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.020503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398279PMC
February 2019

Photoacoustic computed tomography of human extremities.

J Biomed Opt 2019 Feb;24(2):1-8

California Institute of Technology, Caltech Optical Imaging Laboratory, Department of Electrical Eng, United States.

We present a method of imaging angiographic structures in human extremities, including hands, arms, legs, and feet, using a newly developed photoacoustic computed tomography (PACT) system. The system features deep penetration (1.8 cm in muscular tissues) with high spatial and temporal resolutions. Read More

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https://www.spiedigitallibrary.org/journals/journal-of-biome
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http://dx.doi.org/10.1117/1.JBO.24.2.026003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380242PMC
February 2019
10 Reads
2.859 Impact Factor

Special Section Guest Editorial: Translational Biophotonics.

J Biomed Opt 2019 Feb;24(2):1-2

Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road East, University of California.

This guest editorial introduces the special section on Translational Biophotonics. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.021200DOI Listing
February 2019

Micron resolution, high-fidelity three-dimensional vascular optical imaging phantoms.

J Biomed Opt 2019 Feb;24(2):1-4

Wellcome Trust-EPSRC Centre for Interventional and Surgical Sciences, London, United Kingdom.

Microscopic and mesoscale optical imaging techniques allow for three-dimensional (3-D) imaging of biological tissue across millimeter-scale regions, and imaging phantom models are invaluable for system characterization and clinical training. Phantom models that replicate complex 3-D geometries with both structural and molecular contrast, with resolution and lateral dimensions equivalent to those of imaging techniques (<20  μm), have proven elusive. We present a method for fabricating phantom models using a combination of two-photon polymerization (2PP) to print scaffolds, and microinjection of tailored tissue-mimicking materials to simulate healthy and diseased tissue. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.020502DOI Listing
February 2019
1 Read

Dynamic light scattering optical coherence tomography to probe motion of subcellular scatterers.

J Biomed Opt 2019 Feb;24(2):1-7

Ryerson University, Department of Physics, Faculty of Science, Toronto, Canada.

Optical coherence tomography (OCT) is used to provide anatomical information of biological systems but can also provide functional information by characterizing the motion of intracellular structures. Dynamic light scattering OCT was performed on intact, control MCF-7 breast cancer cells and cells either treated with paclitaxel to induce apoptosis or deprived of nutrients to induce oncosis. Autocorrelations (ACs) of the temporal fluctuations of OCT intensity signals demonstrate a significant decrease in decorrelation time after 24 h in both the paclitaxel-treated and nutrient-deprived cell groups but no significant differences between the two groups. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.025002DOI Listing
February 2019
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Integration of a Raman spectroscopy system to a robotic-assisted surgical system for real-time tissue characterization during radical prostatectomy procedures.

J Biomed Opt 2019 Feb;24(2):1-10

Polytechnique Montreal, Montreal, Canada.

Surgical excision of the whole prostate through a radical prostatectomy procedure is part of the standard of care for prostate cancer. Positive surgical margins (cancer cells having spread into surrounding nonresected tissue) occur in as many as 1 in 5 cases and strongly correlate with disease recurrence and the requirement of adjuvant treatment. Margin assessment is currently only performed by pathologists hours to days following surgery and the integration of a real-time surgical readout would benefit current prostatectomy procedures. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.025001DOI Listing
February 2019
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Sensing, monitoring, and release of therapeutics: the translational journey of next generation bandages (Erratum).

J Biomed Opt 2019 Feb;24(2)

Mass General Research Institute, Boston, Massachusetts, United States.

This erratum corrects an error in "Sensing, monitoring, and release of therapeutics: the translational journey of next generation bandages," by Z. Li et al. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.029802DOI Listing
February 2019

Special Section Guest Editorial: Biomedical Imaging and Sensing.

J Biomed Opt 2019 Feb;24(3)

National Taiwan University, Taiwan.

This guest editorial introduces the special section on Biomedical Imaging and Sensing. Read More

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http://dx.doi.org/10.1117/1.JBO.24.3.031001DOI Listing
February 2019

Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins.

J Biomed Opt 2019 Feb;24(2):1-11

University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States.

Intraoperative assessment of breast surgical margins will be of value for reducing the rate of re-excision surgeries for lumpectomy patients. While frozen-section histology is used for intraoperative guidance of certain cancers, it provides limited sampling of the margin surface (typically <1  %   of the margin) and is inferior to gold-standard histology, especially for fatty tissues that do not freeze well, such as breast specimens. Microscopy with ultraviolet surface excitation (MUSE) is a nondestructive superficial optical-sectioning technique that has the potential to enable rapid, high-resolution examination of excised margin surfaces. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.026501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368047PMC
February 2019
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Diagnostic performance of receptor-specific surgical specimen staining correlates with receptor expression level.

J Biomed Opt 2019 Feb;24(2):1-9

Oregon Health and Science University, Department of Biomedical Engineering, Portland, Oregon, United States.

Intraoperative margin assessment is imperative to cancer cure but is a continued challenge to successful surgery. Breast conserving surgery is a relevant example, where a cosmetically improved outcome is gained over mastectomy, but re-excision is required in >25  %   of cases due to positive or closely involved margins. Clinical translation of margin assessment modalities that must directly contact the patient or required administered contrast agents are time consuming and costly to move from bench to bedside. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.026002DOI Listing
February 2019

fNIRS improves seizure detection in multimodal EEG-fNIRS recordings.

J Biomed Opt 2019 Feb;24(5):1-9

Université de Montréal, École Polytechnique de Montréal, Montréal, Québec, Canada.

In the context of epilepsy monitoring, electroencephalography (EEG) remains the modality of choice. Functional near-infrared spectroscopy (fNIRS) is a relatively innovative modality that cannot only characterize hemodynamic profiles of seizures but also allow for long-term recordings. We employ deep learning methods to investigate the benefits of integrating fNIRS measures for seizure detection. Read More

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https://www.spiedigitallibrary.org/journals/journal-of-biome
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http://dx.doi.org/10.1117/1.JBO.24.5.051408DOI Listing
February 2019
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Terahertz spectroscopy of gelatin-embedded human brain gliomas of different grades: a road toward intraoperative THz diagnosis.

J Biomed Opt 2019 Feb;24(2):1-5

Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.

We applied terahertz (THz)-pulsed spectroscopy to study ex vivo the refractive index and absorption coefficient of human brain gliomas featuring different grades, as well as perifocal regions containing both intact and edematous tissues. Glioma samples from 26 patients were considered and analyzed according to further histological examination. In order to fix tissues for the THz measurements, we applied gelatin embedding, which allows for sustaining their THz response unaltered, as compared to that of the freshly excised tissues. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.027001DOI Listing
February 2019
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Cherenkov luminescence imaging of shallow sources in semitransparent media.

J Biomed Opt 2019 Feb;24(2):1-9

Washington University in St. Louis, Department of Radiology, St. Louis, Missouri, United States.

We experimentally investigated the Cherenkov luminescence imaging (CLI) of the isotopes with different beta particles energies (Cu64, F18, Au198, P32, and Br76) in semitransparent biological equivalent media. The main focus of this work is to characterize the CLI when the sources are at the depth comparable with the range of beta particles. The experimental results were compared with Monte Carlo (MC) simulation results to fine tune the simulation parameters to better model the phantom materials. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.026001DOI Listing
February 2019

Impact of hemoglobin breakdown products in the spectral analysis of burn wounds using spatial frequency domain spectroscopy.

J Biomed Opt 2019 Feb;24(2):1-4

University of California Irvine, Beckman Laser Institute and Medical Clinic, Irvine, California, United States.

Burn wounds and wound healing invoke several biological processes that may complicate the interpretation of spectral imaging data. Through analysis of spatial frequency domain spectroscopy data (450 to 1000 nm) obtained from longitudinal investigations using a graded porcine burn wound healing model, we have identified features in the absorption spectrum that appear to suggest the presence of hemoglobin breakdown products, e.g. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.020501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398280PMC
February 2019