304 results match your criteria Journal of Biological Engineering [Journal]


A prototype RFID tag for detecting bumblebee visitations within fragmented landscapes.

J Biol Eng 2019 7;13:13. Epub 2019 Feb 7.

1Royal Botanic Gardens, Kew, Surrey, TW9 3AB UK.

Detecting the arbitrary movements of fast-moving insects under field conditions is notoriously difficult because existing technologies are limited by issues of size, weight, range and cost. Here, we establish proof-of-concept for a prototype long-range, passive radio frequency identification (RFID) tagging system for detecting bumblebees and similar sized insects. The prototype tags, weighing 81 mg (49% of mean bee body weight), were flown by bumblebees in a glasshouse and detected at a distance of 1. Read More

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http://dx.doi.org/10.1186/s13036-019-0143-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367791PMC
February 2019

Quest for cardiovascular interventions: precise modeling and 3D printing of heart valves.

J Biol Eng 2019 6;13:12. Epub 2019 Feb 6.

5Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.

Digitalization of health care practices is substantially manifesting itself as an effective tool to diagnose and rectify complex cardiovascular abnormalities. For cardiovascular abnormalities, precise non-invasive imaging interventions are being used to develop patient specific diagnosis and surgical planning. Concurrently, pre surgical 3D simulation and computational modeling are aiding in the effective surgery and understanding of valve biomechanics, respectively. Read More

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http://dx.doi.org/10.1186/s13036-018-0132-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366048PMC
February 2019

Transcriptome-guided target identification of the TetR-like regulator SACE_5754 and engineered overproduction of erythromycin in .

J Biol Eng 2019 24;13:11. Epub 2019 Jan 24.

1School of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601 China.

Background: Erythromycin A (Er-A) produced by the actinomycete is an important antibiotic extensively used in human medicine. Dissecting of transcriptional regulators and their target genes associated with erythromycin biosynthesis is crucial to obtain erythromycin overproducer strains through engineering of relevant regulatory elements in .

Results: Here, we identified a TetR family transcriptional regulator (TFR), SACE_5754, negatively controlling erythromycin production. Read More

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http://dx.doi.org/10.1186/s13036-018-0135-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346578PMC
January 2019

Hydrophobins: multifunctional biosurfactants for interface engineering.

J Biol Eng 2019 23;13:10. Epub 2019 Jan 23.

1Department of Biomedical Engineering, University of Virginia, Thornton Hall, P.O. Box 400259, Charlottesville, VA 22904 USA.

Hydrophobins are highly surface-active proteins that have versatile potential as agents for interface engineering. Due to the large and growing number of unique hydrophobin sequences identified, there is growing potential to engineer variants for particular applications using protein engineering and other approaches. Recent applications and advancements in hydrophobin technologies and production strategies are reviewed. Read More

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http://dx.doi.org/10.1186/s13036-018-0136-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343262PMC
January 2019

AFM Imaging Reveals Multiple Conformational States of ADAMTS13.

J Biol Eng 2019 22;13. Epub 2019 Jan 22.

Institute of Biomechanics, School of Biosciences and Bioengineering, South China University of Technology, Guangzhou, 510006 China.

Background: ADAMTS13 (A disintegrin and metalloprotease with a thrombospondin type 1 motif 13) cleaves Von Willebrand factor (VWF) to regulate its size, thereby preventing aberrant platelet aggregation and thrombus. Deficiency of ADAMTS13 caused by either genetic mutations or by inhibitory autoantibodies against ADAMTS13 leads to thrombotic thrombocytopenic purpura (TTP). Recently, ADAMTS13 was reported to adopt a "closed" conformation with lower activity and an "open" one resulting from the engagements of VWF D4-CK domains or antibodies to the distal domains of ADAMTS13, or mutations in its spacer domain. Read More

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http://dx.doi.org/10.1186/s13036-018-0102-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343300PMC
January 2019
1 Read

Utilising datasheets for the informed automated design and build of a synthetic metabolic pathway.

J Biol Eng 2019 18;13. Epub 2019 Jan 18.

1Department of Bioengineering, Imperial College London, London, UK.

Background: The automation of modular cloning methodologies permits the assembly of many genetic designs. Utilising characterised biological parts aids in the design and redesign of genetic pathways. The characterisation information held on datasheets can be used to determine whether a biological part meets the design requirements. Read More

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http://dx.doi.org/10.1186/s13036-019-0141-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339355PMC
January 2019
1 Read

Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications.

J Biol Eng 2019 18;13. Epub 2019 Jan 18.

Department of Biological Systems Engineering, University of Nebraska-Lincoln, 231 L.W. Chase Hall, Lincoln, NE 68583-0726 USA.

Background: Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expanded from many tissues, and are being investigated for use in cell therapies. Though MSC therapies have demonstrated some success, none have been FDA approved for clinical use. MSCs lose stemness decreasing therapeutic potential, and face additional barriers decreasing therapeutic efficacy. Read More

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-019-0140-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339289PMC
January 2019
3 Reads

An injectable sulfonated reversible thermal gel for therapeutic angiogenesis to protect cardiac function after a myocardial infarction.

J Biol Eng 2019 17;13. Epub 2019 Jan 17.

1Department of Bioengineering, University of Colorado Denver Anschutz Medical Campus, Aurora, CO 80045 USA.

Background: Cardiovascular disease and myocardial infarction are associated with high mortality and morbidity and a more effective treatment remains a major clinical need. The intramyocardial injection of biomaterials has been investigated as a potential treatment for heart failure by providing mechanical support to the myocardium and reducing stress on cardiomyocytes. Another treatment approach that has been explored is therapeutic angiogenesis that requires careful spatiotemporal control of angiogenic drug delivery. Read More

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-019-0142-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337754PMC
January 2019
3 Reads

Coatings on mammalian cells: interfacing cells with their environment.

J Biol Eng 2019 17;13. Epub 2019 Jan 17.

Chemical and Materials Engineering, University of Kentucky, 177 FPAT, Lexington, KY 40506-0046 USA.

The research community is intent on harnessing increasingly complex biological building blocks. At present, cells represent a highly functional component for integration into higher order systems. In this review, we discuss the current application space for cellular coating technologies and emphasize the relationship between the target application and coating design. Read More

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http://dx.doi.org/10.1186/s13036-018-0131-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337841PMC
January 2019
1 Read

Bioengineered models to study tumor dormancy.

J Biol Eng 2019 10;13. Epub 2019 Jan 10.

Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203 USA.

The onset of cancer metastasis is the defining event in cancer progression when the disease is considered lethal. The ability of metastatic cancer cells to stay dormant for extended time periods and reawaken at later stages leading to disease recurrence makes treatment of metastatic disease extremely challenging. The tumor microenvironment plays a critical role in deciding the ultimate fate of tumor cells, yet the mechanisms by which this occurs, including dormancy, is not well understood. Read More

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http://dx.doi.org/10.1186/s13036-018-0137-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327399PMC
January 2019

Follicle development as an orchestrated signaling network in a 3D organoid.

J Biol Eng 2019 9;13. Epub 2019 Jan 9.

Department of Biomedical Engineering, University of Michigan, 2126 Lurie Biomedical Engineering, 1101 Beal Avenue, Ann Arbor, MI 48109 USA.

The ovarian follicle is the structural and functional unit of the ovary, composed of the female gamete (the oocyte) and supportive somatic cells. Follicles are not only the source of a female's germ cell supply, but also secrete important hormones necessary for proper endocrine function. Folliculogenesis, the growth and maturation of the follicular unit, is a complex process governed by both intrafollicular crosstalk and pituitary-secreted hormones. Read More

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http://dx.doi.org/10.1186/s13036-018-0134-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327556PMC
January 2019
4 Reads

The effects of low-dose radiation on articular cartilage: a review.

J Biol Eng 2019 7;13. Epub 2019 Jan 7.

Department of Bioengineering, Clemson University, 301 Rhodes Research Center, Clemson, SC 29634 USA.

Articular cartilage is a specialized connective tissue, predominately composed of water, collagen, and proteoglycans, that provides a smooth, lubricated surface for articulation in joints. It has long been considered radioinsensitive and therefore unaffected by exposure to radiation in medical settings. Due to the increased amount of yearly radiation exposure through radiotherapy and ionizing radiation diagnostic procedures, there has been a renewed interest in how radioinsensitive articular cartilage actually is. Read More

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http://dx.doi.org/10.1186/s13036-018-0125-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322226PMC
January 2019

Engineered Models of Tumor Dormancy and Reactivation.

J Biol Eng 2018 27;12:37. Epub 2018 Dec 27.

1Department of Biomedical Engineering, University of Delaware, 150 Academy Street, 161 Colburn Lab, Newark, DE 19716 USA.

Metastatic recurrence is a major hurdle to overcome for successful control of cancer-associated death. Residual tumor cells in the primary site, or disseminated tumor cells in secondary sites, can lie in a dormant state for long time periods, years to decades, before being reactivated into a proliferative growth state. The microenvironmental signals and biological mechanisms that mediate the fate of disseminated cancer cells with respect to cell death, single cell dormancy, tumor mass dormancy and metastatic growth, as well as the factors that induce reactivation, are discussed in this review. Read More

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http://dx.doi.org/10.1186/s13036-018-0120-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307145PMC
December 2018

Tissue engineering strategies for the induction of angiogenesis using biomaterials.

J Biol Eng 2018 27;12:36. Epub 2018 Dec 27.

2Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

Angiogenesis is touted as a fundamental procedure in the regeneration and restoration of different tissues. The induction of de novo blood vessels seems to be vital to yield a successful cell transplantation rate loaded on various scaffolds. Scaffolds are natural or artificial substances that are considered as one of the means for delivering, aligning, maintaining cell connection in a favor of angiogenesis. Read More

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http://dx.doi.org/10.1186/s13036-018-0133-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307144PMC
December 2018

CD24 identifies nucleus pulposus progenitors/notochordal cells for disc regeneration.

J Biol Eng 2018 22;12:35. Epub 2018 Dec 22.

1Department of Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Er Road, Shanghai, 200025 China.

Background: Cell-based therapy by transplantation of nucleus pulposus (NP) progenitor/notochordal cells has been proposed as a promising way to halt and reverse the progression of disc degeneration. Although some studies have provided a broad panel of potential markers associated with the phenotype of notochordal cells, suitability of these markers for isolation of notochordal cells for the treatment of disc degeneration is unclear.

Results: Here, we found that the number of CD24-positive NP cells significantly decreased with increasing severity of disc degeneration. Read More

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http://dx.doi.org/10.1186/s13036-018-0129-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303933PMC
December 2018
2 Reads

Online evaluation of the metabolic activity of on (poly)galacturonic acid.

J Biol Eng 2018 18;12:34. Epub 2018 Dec 18.

1AVT - Biochemical Engineering, RWTH Aachen University, Jochen Büchs, Forckenbeckstr. 51, 52074 Aachen, Germany.

Background: Pectin is a rather complex and highly branched polysaccharide strengthening the plant cell wall. Thus, many different pectinases are required for an efficient microbial conversion of biomass waste streams with a high pectin content like citrus peel, apple pomace or sugar beet pulp. The screening and optimization of strains growing on pectic substrates requires both, quantification of the residual substrate and an accurate determination of the enzymatic activity. Read More

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http://dx.doi.org/10.1186/s13036-018-0128-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299674PMC
December 2018

Delivery systems of CRISPR/Cas9-based cancer gene therapy.

J Biol Eng 2018 18;12:33. Epub 2018 Dec 18.

Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale G.B. Morgagni 50 -, 50134 Florence, Italy.

CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is today one of the most reliable method for gene-editing, supporting previous gene therapies technologies such as TALEN, Meganucleases and ZFNs. There is a growing up number of manuscripts reporting several successful gene-edited cancer cell lines, but the real challenge is to translate this technique to the clinical practice. While treatments for diseases based on a single gene mutation is closer, being possible to target and repair the mutant allele in a selective way generating specific guide RNAs (gRNAs), many steps need to be done to apply CRISPR to face cancer. Read More

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http://dx.doi.org/10.1186/s13036-018-0127-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299643PMC
December 2018

Lymphatic Tissue Engineering and Regeneration.

J Biol Eng 2018 17;12:32. Epub 2018 Dec 17.

1Department of Aerospace and Mechanical Engineering, Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN 46556 USA.

The lymphatic system is a major circulatory system within the body, responsible for the transport of interstitial fluid, waste products, immune cells, and proteins. Compared to other physiological systems, the molecular mechanisms and underlying disease pathology largely remain to be understood which has hindered advancements in therapeutic options for lymphatic disorders. Dysfunction of the lymphatic system is associated with a wide range of disease phenotypes and has also been speculated as a route to rescue healthy phenotypes in areas including cardiovascular disease, metabolic syndrome, and neurological conditions. Read More

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-018-0122-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296077PMC
December 2018
11 Reads

An electrically-controlled programmable microfluidic concentration waveform generator.

J Biol Eng 2018 14;12:31. Epub 2018 Dec 14.

1Department of Electrical & Computer Engineering, University of California - Davis, Davis, CA 95616 USA.

Background: Biological systems have complicated environmental conditions that vary both spatially and temporally. It becomes necessary to impose time-varying soluble factor concentrations to study such systems, including cellular responses to pharmaceuticals, inflammation with waxing and waning cytokine concentrations, as well as circadian rhythms and their metabolic manifestations. There is therefore a need for platforms that can achieve time-varying concentrations with arbitrary waveforms. Read More

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http://dx.doi.org/10.1186/s13036-018-0126-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295081PMC
December 2018

Cell surface engineering and application in cell delivery to heart diseases.

J Biol Eng 2018 4;12:28. Epub 2018 Dec 4.

Division of Cardio-Thoracic Surgery, Department of Surgery, University of Arizona College of Medicine, Room 4302D, 1501 N Campbell Ave, Tucson, Arizona 85724 USA.

Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. Due to their secretory functions, differentiation capabilities, specific homing effects through chemotaxis, distinctive therapeutic potentials, and expandability, cells have become an attractive reagent for advanced therapeutic strategies. Therefore, the ability to modify cells and manipulate their functions according to intended therapeutic designs has been the central scientific interest in the field of biomedical research. Read More

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http://dx.doi.org/10.1186/s13036-018-0123-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278044PMC
December 2018
1 Read

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter.

J Biol Eng 2018 4;12:30. Epub 2018 Dec 4.

1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716 China.

Background: Silkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of silkworm transgenic expression and gene therapy. However, the low expression and specificity of natural promoters limit the applications of genetic engineering. Read More

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http://dx.doi.org/10.1186/s13036-018-0121-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280533PMC
December 2018
2 Reads

Imaging retinal melanin: a review of current technologies.

J Biol Eng 2018 4;12:29. Epub 2018 Dec 4.

1Morgridge Institute for Research, Madison, WI USA.

The retinal pigment epithelium (RPE) is essential to the health of the retina and the proper functioning of the photoreceptors. The RPE is rich in melanosomes, which contain the pigment melanin. Changes in RPE pigmentation are seen with normal aging and in diseases such as albinism and age-related macular degeneration. Read More

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http://dx.doi.org/10.1186/s13036-018-0124-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280494PMC
December 2018

Laser MICROSAMPLING of soil microbial community.

J Biol Eng 2018 28;12:27. Epub 2018 Nov 28.

Research Center "Crystallography and Photonics" RAS, Institute of Photonic Technologies, 142190, Troitsk, Moscow, Russia.

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-018-0117-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263061PMC
November 2018
7 Reads

In vitro culture expansion impairs chondrogenic differentiation and the therapeutic effect of mesenchymal stem cells by regulating the unfolded protein response.

J Biol Eng 2018 20;12:26. Epub 2018 Nov 20.

4National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064 China.

In vitro expansion of mesenchymal stem cells (MSCs) has been implicated in loss of multipotency, leading to impaired chondrogenic potential and an eventual therapeutic effect, as reported in our previous study. However, the precise regulatory mechanism is still unclear. Here, we demonstrate that endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) were involved in transformation of MSCs induced by in vitro culture based on the comparative profiling of in vitro cultured bone marrow MSCs at passage 3 (P3 BMSCs) vs. Read More

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http://dx.doi.org/10.1186/s13036-018-0119-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245887PMC
November 2018
1 Read

Bioconversion of cheese whey permeate into fungal oil by .

J Biol Eng 2018 14;12:25. Epub 2018 Nov 14.

1Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616 USA.

Background: Oleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus was cultivated in whey permeate, a byproduct from cheese production, to produce an oil-rich fungal biomass. Response surface methodology was used to optimize the fermentation conditions such as pH and temperature for increased biomass yield and lipid accumulation. Read More

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http://dx.doi.org/10.1186/s13036-018-0116-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237013PMC
November 2018

Prevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments.

J Biol Eng 2018 13;12:24. Epub 2018 Nov 13.

1Institute for Clinical & Experimental Surgery, Saarland University, 66421 Homburg/Saar, Germany.

Background: The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments (ad-MVF) may serve as vascularization units. In contrast to SVF single cells, they represent a mixture of intact arteriolar, capillary and venular vessel segments. Read More

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http://dx.doi.org/10.1186/s13036-018-0118-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234670PMC
November 2018

The genetic insulator RiboJ increases expression of insulated genes.

J Biol Eng 2018 29;12:23. Epub 2018 Oct 29.

1Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185 USA.

A primary objective of synthetic biology is the construction of genetic circuits with behaviors that can be predicted based on the properties of the constituent genetic parts from which they are built. However a significant issue in the construction of synthetic genetic circuits is a phenomenon known as context dependence in which the behavior of a given part changes depending on the choice of adjacent or nearby parts. Interactions between parts compromise the modularity of the circuit, impeding the implementation of predictable genetic constructs. Read More

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-018-0115-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206723PMC
October 2018
1 Read

Optimization of fermentation conditions for an strain engineered using the response surface method to produce a novel therapeutic DNA vaccine for rheumatoid arthritis.

J Biol Eng 2018 10;12:22. Epub 2018 Oct 10.

Department of Immunology and National Center for Biomedicine Analysis, Beijing 307 Hospital, No.8, Dongda Ave, Fengtai District, Beijing, 100071 People's Republic of China.

Background: Fermentation condition optimization and nutrients screening are of equal importance for efficient production of plasmid DNA vaccines. This directly affects the downstream purification and final quality and yield of plasmid DNA vaccines. The present study aimed to optimize the fermentation conditions for high-throughput production of therapeutic DNA vaccine pcDNA-CCOL2A1 by engineered DH5α, using the response surface method (RSM). Read More

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http://dx.doi.org/10.1186/s13036-018-0110-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180442PMC
October 2018
1 Read

Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures.

J Biol Eng 2018 1;12:21. Epub 2018 Oct 1.

1Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Països Catalans 26, 43007 Tarragona, Spain.

Background: The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. Here, the response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon with collagen and fibronectin functionalization has been studied. Read More

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http://dx.doi.org/10.1186/s13036-018-0111-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166296PMC
October 2018

Native-mimicking in vitro microenvironment: an elusive and seductive future for tumor modeling and tissue engineering.

J Biol Eng 2018 12;12:20. Epub 2018 Sep 12.

Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99210 USA.

Human connective tissues are complex physiological microenvironments favorable for optimal survival, function, growth, proliferation, differentiation, migration, and death of tissue cells. Mimicking native tissue microenvironment using various three-dimensional (3D) tissue culture systems in vitro has been explored for decades, with great advances being achieved recently at material, design and application levels. These achievements are based on improved understandings about the functionalities of various tissue cells, the biocompatibility and biodegradability of scaffolding materials, the biologically functional factors within native tissues, and the pathophysiological conditions of native tissue microenvironments. Read More

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http://dx.doi.org/10.1186/s13036-018-0114-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6136168PMC
September 2018
1 Citation

Machine learning analysis of microbial flow cytometry data from nanoparticles, antibiotics and carbon sources perturbed anaerobic microbiomes.

J Biol Eng 2018 12;12:19. Epub 2018 Sep 12.

Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania, Urbana, 61801 USA.

Background: Flow cytometry, with its high throughput nature, combined with the ability to measure an increasing number of cell parameters at once can surpass the throughput of prevalent genomic and metagenomic approaches in the study of microbiomes. Novel computational approaches to analyze flow cytometry data will result in greater insights and actionability as compared to traditional tools used in the analysis of microbiomes. This paper is a demonstration of the fruitfulness of machine learning in analyzing microbial flow cytometry data generated in anaerobic microbiome perturbation experiments. Read More

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http://dx.doi.org/10.1186/s13036-018-0112-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134764PMC
September 2018
10 Reads

Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation.

J Biol Eng 2018 12;12:17. Epub 2018 Sep 12.

1College of Bioengineering & School of Life Sciences, Chongqing University, Chongqing, 400044 China.

Background: Generation of reactive oxygen species (ROS), triggered by ultraviolet radiation (UVR), is associated with carcinogenesis of the skin. UV irradiation induced superoxide anion (O2) is the key ROS involved in the cellular damage. The cytoprotective efficacy of an unknown anti-oxidant compound can be evaluated by analyzing the production of O2 from treated cells. Read More

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http://dx.doi.org/10.1186/s13036-018-0099-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134779PMC
September 2018
5 Reads

Engineering and monitoring cellular barrier models.

J Biol Eng 2018 12;12:18. Epub 2018 Sep 12.

Institut de Microelectrònica de Barcelona, IMB-CNM (CSIC), 08193, Bellaterra, Barcelona, Spain.

Epithelia and endothelia delineate tissue compartments and control their environments by regulating the passage of ions and solutes. This barrier function is essential for the development and maintenance of multicellular organisms, and its dysfunction is associated with numerous human diseases. Recent advances in biomaterials and microfabrication technologies have evolved in vitro approaches for modelling biological barriers. Read More

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-018-0108-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134550PMC
September 2018
10 Reads

Bio-hybrid inorganic microparticles derived from CO for highly efficient and selective removal of antibiotics.

J Biol Eng 2018 6;12:16. Epub 2018 Sep 6.

1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 136-701 Republic of Korea.

Background: Antibiotics, which are the most important medication in human history, have brought global concerns due to their potential risk to human health and environment by accelerating the development of drug-resistant bacteria, and accumulating in the food chain system. Among antibiotics, oxytetracycline (OTC) is widely used in aquaculture, and its potential risk of toxicity to human by bioaccumulation has been reported. Therefore, the effective removal of OTC is highly needed. Read More

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http://dx.doi.org/10.1186/s13036-018-0113-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127930PMC
September 2018
1 Read

A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator.

J Biol Eng 2018 14;12:15. Epub 2018 Aug 14.

1Department of Bioengineering, Imperial College London, London, SW7 2AZ UK.

Background: Effects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one paradigm, endothelial cells are cultured in devices that produce spatially varying shear stress profiles, and the local profile is compared with the properties of cells at the same position. Read More

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http://dx.doi.org/10.1186/s13036-018-0107-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092814PMC
August 2018
13 Reads

Synthesis of control unit for future biocomputer.

J Biol Eng 2018 14;12:14. Epub 2018 Aug 14.

Department of Electrical Engineering, National Chung Hsing University, Taichung, 402 Taiwan.

Background: Synthesis of a variety of biological circuits for specific functional purposes has made a tremendous progress in recent years. The ultimate goal of combining molecular biology and engineering is to realize a functional biocomputer. To address this challenge, all previous efforts work toward building up the bio-computer as the ultimate goal. Read More

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http://dx.doi.org/10.1186/s13036-018-0109-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092829PMC
August 2018
18 Reads

A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl.

J Biol Eng 2018 3;12:12. Epub 2018 Aug 3.

Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455 USA.

Background: While hypoxia has been well-studied in various tumor microenvironments, its role in cancer cell dormancy is poorly understood, in part due to a lack of well-established in vitro and in vivo models. Hypoxic conditions under conventional hypoxia chambers are relatively unstable and cannot be maintained during characterization outside the chamber since normoxic response is quickly established. To address this challenge, we report a robust in vitro cancer dormancy model under a hypoxia-mimicking microenvironment using cobalt chloride (CoCl), a hypoxia-mimetic agent, which stabilizes hypoxia inducible factor 1-alpha (HIF1α), a major regulator of hypoxia signaling. Read More

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http://dx.doi.org/10.1186/s13036-018-0106-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091074PMC
August 2018
1 Read

Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications.

J Biol Eng 2018 14;12:13. Epub 2018 Aug 14.

1Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany.

Background: Biosafety is a key aspect in the international Genetically Engineered Machine (iGEM) competition, which offers student teams an amazing opportunity to pursue their own research projects in the field of Synthetic Biology. iGEM projects often involve the creation of genetically engineered bacterial strains. To minimize the risks associated with bacterial release, a variety of biosafety systems were constructed, either to prevent survival of bacteria outside the lab or to hinder horizontal or vertical gene transfer. Read More

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http://dx.doi.org/10.1186/s13036-018-0105-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090650PMC
August 2018
4 Reads

Anti-CRISPR-based biosensors in the yeast .

J Biol Eng 2018 1;12:11. Epub 2018 Aug 1.

School of Life Science and Technology, 2 Yikuang Street, Nan Gang DistrictHarbin, 150080 People's Republic of China.

Background: Anti-CRISPR proteins are expressed by phages as a reaction to the bacterial CRISPR-Cas defense system. Recently, the structures of anti-CRISPR proteins have been determined, and their diverse functions have been clarified. Anti-CRISPR proteins such as LmAcrIIA2 and LmAcrIIA4 interact with the SpCas9:gRNA system and occlude the protospacer adjacent motif (PAM) recognition site, thereby preventing SpCas9:gRNA from binding to the DNA. Read More

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http://dx.doi.org/10.1186/s13036-018-0101-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090965PMC
August 2018
16 Reads

Manipulation of two regulatory genes for efficient production of chromomycins in .

J Biol Eng 2018 7;12. Epub 2018 Jun 7.

1Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322-4105 USA.

Background: Regulatory genes play critical roles in natural product biosynthetic pathways. Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain for production of these molecules by manipulating the regulatory genes. Read More

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http://dx.doi.org/10.1186/s13036-018-0103-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992853PMC
June 2018
12 Reads

Improved biodegradability of hardly-decomposable wastewaters from petrochemical industry through photo-Fenton method and determination of optimum operational conditions by response surface methodology.

J Biol Eng 2018 20;12:10. Epub 2018 Jun 20.

Faculty of Civil, Water and Environmental Engineering, Shahid Beheshti University A.C, Abbaspour Boulevard, Hakimieh, Tehranpars, Tehran, 17765-1719 Iran.

Background: Petrochemical wastewaters are highly polluting due to having various destructive materials such as aromatic hydrocarbons and heavy metal ions. Therefore, they need to be treated before disposal to the environment. However, due to low biodegradability, applying common treatment methods such as activated sludge is not feasible for these wastewaters. Read More

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http://dx.doi.org/10.1186/s13036-018-0104-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011265PMC
June 2018
8 Reads

Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology.

J Biol Eng 2018 10;12. Epub 2018 May 10.

1Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.

Background: Coral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong colors in ambient light. This endows CPs with certain advantages over FPs, such as instrument-free detection uncomplicated by ultra-violet light damage or background fluorescence, efficient Förster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Read More

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http://dx.doi.org/10.1186/s13036-018-0100-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946454PMC
May 2018
2 Reads

RSM based optimization of nutritional conditions for cellulase mediated Saccharification by .

J Biol Eng 2018 3;12. Epub 2018 May 3.

1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.

Background: Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of was explored while attacking poplar twigs. Read More

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http://dx.doi.org/10.1186/s13036-018-0097-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934882PMC
May 2018
3 Reads

The rat pancreatic body tail as a source of a novel extracellular matrix scaffold for endocrine pancreas bioengineering.

J Biol Eng 2018 27;12. Epub 2018 Apr 27.

1Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035 China.

Background: Regenerative medicine and tissue engineering are promising approaches for organ transplantation. Extracellular matrix (ECM) based scaffolds obtained through the decellularization of natural organs have become the preferred platform for organ bioengineering. In the field of pancreas bioengineering, acellular scaffolds from different animals approximate the biochemical, spatial and vascular relationships of the native extracellular matrix and have been proven to be a good platform for recellularization and in vitro culture. Read More

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http://dx.doi.org/10.1186/s13036-018-0096-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923185PMC
April 2018
9 Reads

Enhancing the functional output of transplanted islets in diabetic mice using a drug-eluting scaffold.

J Biol Eng 2018 18;12. Epub 2018 Apr 18.

Department of Heptobiliary Surgery, Yinzhou People's Hospital, Ningbo, Zhejiang China.

Background: Islet transplantation is increasingly used in the diabetic patients to control the blood glucose level. However, the functional output of transplanted islets remains hampered due to the local inflammation, loss of islets, etc. To that end, in this study we explored to enhance the functional output of transplanted islets in diabetic mice by employing a drug-eluting scaffold with a payload of interleukin 4 (IL-4). Read More

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http://dx.doi.org/10.1186/s13036-018-0098-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907474PMC
April 2018
6 Reads

Disposable microfluidic micromixers for effective capture of oocysts from water samples.

J Biol Eng 2018 27;12. Epub 2018 Mar 27.

1Future Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes Campus, Mawson Lakes, South Australia Australia.

Background: Protecting drinking water supplies from pathogens such as is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determined by the effectiveness of the concentration and separation methods used. The purpose of this study is to develop micromixers able to specifically isolate and concentrate , while allowing in situ analysis. Read More

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http://dx.doi.org/10.1186/s13036-018-0095-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870503PMC
March 2018
1 Read

Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli.

J Biol Eng 2018 15;12. Epub 2018 Mar 15.

1Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany.

Background: Tryptophan derivatives such as 5-hydroxytryptophan (5HTP) and serotonin are valuable molecules with pharmaceutical interest. 5HTP is presently mainly obtained by extraction from the plant and serotonin is produced by chemical synthesis. A simple biotechnological method for the production of these compounds is desired. Read More

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https://jbioleng.biomedcentral.com/articles/10.1186/s13036-0
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http://dx.doi.org/10.1186/s13036-018-0094-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856393PMC
March 2018
10 Reads

Synthesis, secretion, function, metabolism and application of natriuretic peptides in heart failure.

J Biol Eng 2018 12;12. Epub 2018 Jan 12.

1Department of Geriatric Cardiology, Chinese People's Liberation Army General Hospital, Beijing, 100853 China.

As a family of hormones with pleiotropic effects, natriuretic peptide (NP) system includes atrial NP (ANP), B-type NP (BNP), C-type NP (CNP), dendroaspis NP and urodilatin, with NP receptor-A (guanylate cyclase-A), NP receptor-B (guanylate cyclase-B) and NP receptor-C (clearance receptor). These peptides are genetically distinct, but structurally and functionally related for regulating circulatory homeostasis in vertebrates. In humans, ANP and BNP are encoded by NP precursor A (NPPA) and NPPB genes on chromosome 1, whereas CNP is encoded by NPPC on chromosome 2. Read More

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http://dx.doi.org/10.1186/s13036-017-0093-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766980PMC
January 2018
10 Reads

Evaluation of green tea extract as a safe personal hygiene against viral infections.

J Biol Eng 2018 8;12. Epub 2018 Jan 8.

Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.

Background: Viral infections often pose tremendous public health concerns as well as economic burdens. Despite the availability of vaccines or antiviral drugs, personal hygiene is considered as effective means as the first-hand measure against viral infections. The green tea catechins, in particular, epigallocatechin-3-gallate (EGCG), are known to exert potent antiviral activity. Read More

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http://dx.doi.org/10.1186/s13036-017-0092-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759362PMC
January 2018
8 Reads

Re-using biological devices: a model-aided analysis of interconnected transcriptional cascades designed from the bottom-up.

J Biol Eng 2017 14;11:50. Epub 2017 Dec 14.

Laboratory of Bioinformatics, Mathematical Modelling and Synthetic Biology, Department of Electrical, Computer and Biomedical Engineering, University of Pavia, 27100 Pavia, Italy.

Background: The study of simplified, ad-hoc constructed model systems can help to elucidate if quantitatively characterized biological parts can be effectively re-used in composite circuits to yield predictable functions. Synthetic systems designed from the bottom-up can enable the building of complex interconnected devices via rational approach, supported by mathematical modelling. However, such process is affected by different, usually non-modelled, unpredictability sources, like cell burden. Read More

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http://dx.doi.org/10.1186/s13036-017-0090-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729246PMC
December 2017
6 Reads