52,833 results match your criteria Journal of Bacteriology[Journal]


A double strand break does not promote pilin antigenic variation.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611

The major subunit of the Type IV pilus (T4p) of undergoes Antigenic variation (Av) dependent on a guanine quadruplex (G4) DNA structure located upstream of the pilin gene. Since the presence of G4 DNA induces genome instability in both eukaryotic and prokaryotic chromosomes, we tested whether a double strand break (DSB) at the site of the G4 sequence can substitute for G4 directed pilin Av. The G4 motif was replaced by an I-SceI cut site and the cut site was also introduced to locations near the origin of replication and the terminus. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00256-19
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http://dx.doi.org/10.1128/JB.00256-19DOI Listing
April 2019
6 Reads

Copper-induced expression of a transmissible lipoprotein intramolecular transacylase alters lipoprotein acylation and the Toll-like receptor 2 response to .

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA

Bacterial lipoproteins are globular proteins anchored to the extracytoplasmic surfaces of cell membranes through lipidation at a conserved -terminal cysteine. Lipoproteins contribute to an array of important cellular functions for bacteria, as well as being a focal point for innate immune system recognition through binding to Toll-like receptor 2 (TLR2) heterodimer complexes. Although lipoproteins are conserved among nearly all classes of bacteria, the presence and type of α-amino-linked acyl chain is highly variable and even strain specific within a given bacterial species. Read More

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http://dx.doi.org/10.1128/JB.00195-19DOI Listing
April 2019
1 Read

Acetate metabolism and the inhibition of bacterial growth by acetate.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Univ. Grenoble Alpes, Inria, 38000 Grenoble, France.

During aerobic growth on glucose, excretes acetate, a mechanism called "overflow metabolism". At high concentrations, the secreted acetate inhibits growth. Several mechanisms have been proposed for explaining this phenomenon, but a thorough analysis is hampered by the diversity of experimental conditions and strains used in these studies. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00147-19
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http://dx.doi.org/10.1128/JB.00147-19DOI Listing
April 2019
1 Read

Guanidine-riboswitch-regulated efflux transporters protect bacteria against ionic liquid toxicity.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

The Joint BioEnergy Institute, Emeryville, California 94608, USA

Plant cell walls contain a renewable, nearly limitless supply of sugar that could be used to support microbial production of commodity chemicals and biofuels. Imidazolium ionic liquid (IIL) solvents are among the best reagents for gaining access to the sugars in this otherwise recalcitrant biomass. However, the sugars from IIL-treated biomass are inevitably contaminated with residual IILs that inhibit growth in bacteria and yeast, blocking biochemical production by these organisms. Read More

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http://dx.doi.org/10.1128/JB.00069-19DOI Listing

requires the DNA-specific endonuclease EndA to degrade eDNA to disperse from the biofilm.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Binghamton Biofilm Research Center, Department of Biological Sciences, Binghamton University, Binghamton, NY 13902

The dispersion of biofilms is an active process resulting in the release of planktonic cells from the biofilm structure. While much is known about the process of dispersion cue perception and the subsequent modulation of the c-di-GMP pool, little is known about subsequent events resulting in the release of cells from the biofilm. Given that dispersion coincides with void formation and an overall erosion of the biofilm structure, we asked whether dispersion involves degradation of the biofilm matrix. Read More

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http://dx.doi.org/10.1128/JB.00059-19DOI Listing

25 Annual Midwest Microbial Pathogenesis Conference.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Department of Microbiology and Immunology, University of Iowa.

The 25th Annual Midwest Microbial Pathogenesis Conference (MMPC) was held at the University of Iowa from September 28-30, 2018. The conference has a long-standing tradition of providing scientists from the Midwest with a forum to present and discuss cutting edge advances in microbial pathogenesis with particular focus on bacterial interactions with the environment, host, and other microbes. This commentary summarizes the genesis of the MMPC, topics presented at the conference, and manuscripts found in the special MMPC section of this issue. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00239-19
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http://dx.doi.org/10.1128/JB.00239-19DOI Listing
April 2019
1 Read

Impact of FiuA outer-membrane receptor polymorphism on the resistance of towards peptidoglycan lipid II-targeting PaeM pyocins.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.

Certain strains produce a homolog of colicin M, named PaeM, that specifically inhibits peptidoglycan biosynthesis of susceptible strains by hydrolyzing the lipid II intermediate precursor. Two variants of this pyocin were identified whose sequences mainly differed in the N-terminal protein moiety, the region involved in the binding to the FiuA outer membrane receptor and translocation into the periplasm. The antibacterial activity of these two variants, PaeM1 and PaeM2, was tested against various strains comprising reference strains PAO1 and PA14, PaeM-producing strains and sixty clinical isolates. Read More

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http://dx.doi.org/10.1128/JB.00164-19DOI Listing
April 2019
2 Reads

Competence Beyond Genes: Filling in the Details of the Pneumococcal Competence Transcriptome by a Systems Approach.

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL USA.

DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen, A study in this issue (Slager , J Bacteriol doi: 10.1128/JB.00780-18) combines deep-genome annotation and high-resolution transcriptome analyses to considerably extend the previous model of temporal regulation of competence at the operon and component-gene levels. Read More

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http://dx.doi.org/10.1128/JB.00238-19DOI Listing

Functioning of mycobacterial heat-shock repressors requires the master virulence regulator PhoP.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

CSIR-Institute of Microbial Technology, Sector 39 A, Chandigarh 160036, India

A hallmark feature of (Mtb) pathogenesis lies in the ability of the pathogen to survive within the macrophages under a stressful environment. Thus, coordinated regulation of stress proteins is critically important for an effective adaptive response of Mtb, failure to which results in elevated immune recognition of the tubercle bacilli with reduced survival during chronic infections. Here, we show that virulence regulator PhoP impacts on global regulation of heat-shock proteins, which protect Mtb against stress generated by macrophages during infection. Read More

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http://dx.doi.org/10.1128/JB.00013-19DOI Listing

φSa3mw Prophage as a Molecular Regulatory Switch of β-toxin Production.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

Department of Microbiology and Immunology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA

Phage regulatory switches (phage-RSs) are a newly described form of active lysogeny where prophages function as regulatory mechanisms for expression of chromosomally-encoded, bacterial genes. In φSa3int is a widely-distributed family of prophages that integrate into the β-toxin structural gene effectively inactivating it. However, β-toxin producing strains often arise during infections and are more virulent in experimental infective endocarditis and pneumonia infections. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00766-18
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http://dx.doi.org/10.1128/JB.00766-18DOI Listing
April 2019
5 Reads

Dissection of the Hydrogen Metabolism of the Enterobacterium : Identification of a Formate-Dependent and Essential Formate Hydrogenlyase Complex Exhibiting Phylogenetic Similarity to Complex I.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle/Saale, Germany

is a non-pathogenic enterobacterium that was isolated from a vacuum cleaner on the island of Guam. It has one H-oxidizing Hyd-2-type hydrogenase (Hyd), and encodes a H-evolving Hyd that is most similar to the uncharacterized formate hydrogenlyase (FHL-2 ) complex. The FHL-2 complex is predicted to have 5 membrane-integral and between 4-5 cytoplasmic subunits. Read More

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http://dx.doi.org/10.1128/JB.00160-19DOI Listing

Proteomic analysis of the iron starvation response reveals PrrF sRNA-dependent iron regulation of twitching motility, amino acid metabolism, and zinc homeostasis proteins.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

University of Maryland, Baltimore, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, Maryland 21201

Iron is a critical nutrient for most microbial pathogens, and the immune system exploits this requirement by sequestering iron. The opportunistic pathogen exhibits a high requirement for iron yet an exquisite ability to overcome iron deprivation during infection. Upon iron starvation, induces the expression of several high affinity iron acquisition systems, as well as the PrrF sRNAs that mediate an iron-sparing response. Read More

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http://dx.doi.org/10.1128/JB.00754-18DOI Listing

Identification of novel Spx regulatory pathways in uncovers a close relationship between the CtsR and Spx regulons.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

Department of Microbiology, Cornell University, Ithaca, NY 14853, US

In , the Spx transcription factor controls a large regulon in response to disulfide, heat, and cell wall stresses. The regulatory mechanisms that activate the Spx regulon are remarkably complex, and involve changes in transcription, proteolysis, and post-translational modifications. To identify genes involved in Spx regulation, we performed a transposon screen for mutations affecting expression of , a Spx-dependent gene. Read More

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http://dx.doi.org/10.1128/JB.00151-19DOI Listing

Flagellar stators activate a diguanylate cyclase to inhibit flagellar stators.

Authors:
Daniel B Kearns

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

Department of Biology, Indiana University, Bloomington IN 47405

The bacterial secondary metabolite cyclic-di-GMP is a widespread, cytoplasmic signal that promotes a physiological transition in which motility is inhibited and biofilm formation is activated.…. Read More

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http://dx.doi.org/10.1128/JB.00186-19DOI Listing

Pathoadaptive alteration of biofilm formation in response to the gallbladder environment.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, Ohio, USA.

Typhoid fever, a human-specific disease, is primarily caused by the pathogen serovar Typhi ( Typhi). It is estimated that 3-5% of people infected with typhoid fever become chronic carriers. Studies have demonstrated that a mechanism of chronic carriage involves biofilm formation on gallstone surfaces. Read More

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http://dx.doi.org/10.1128/JB.00774-18DOI Listing
April 2019
1 Read

complex contact-dependent growth inhibition systems mediate interbacterial competition.

J Bacteriol 2019 Apr 8. Epub 2019 Apr 8.

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536

species, including opportunistic pathogens in the complex (Bcc), encode genes to produce contact-dependent growth inhibition (CDI) system proteins. CDI is a phenomenon in which Gram-negative bacteria use the toxic C-terminus of a polymorphic surface-exposed exoprotein, BcpA, to inhibit the growth of susceptible bacteria upon direct cell-cell contact. Production of a small immunity protein, BcpI, prevents autoinhibition. Read More

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http://dx.doi.org/10.1128/JB.00012-19DOI Listing
April 2019
1 Read

Ethanol stimulates trehalose production through a SpoT-DksA-AlgU dependent pathway in .

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

frequently resides among ethanol-producing microbes, making its response to these microbially-produced concentrations of ethanol relevant to understanding its biology. Our transcriptome analysis found that genes involved in trehalose metabolism were induced by low concentrations of ethanol, and biochemical assays showed levels of intracellular trehalose increased significantly upon growth with ethanol. The increase in trehalose was dependent on the TreYZ pathway, but not other trehalose metabolic enzymes TreS or TreA. Read More

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http://dx.doi.org/10.1128/JB.00794-18DOI Listing
April 2019
1 Read

Human bile-mediated regulation of curli fimbriae.

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA

Typhoid fever is caused primarily by serovar Typhi ( Typhi). Approximately 3-5% of individuals infected with Typhi become chronic carriers with the gallbladder (GB) as the site of persistence, as gallstones within the GB are a platform on which the bacteria form a biofilm. Typhi is a human-restricted pathogen; therefore, asymptomatic carriers represent a critical reservoir for further spread of disease. Read More

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http://dx.doi.org/10.1128/JB.00055-19DOI Listing
April 2019
1 Read

Crystal structure of VapBC-1 from nontypeable and the effect of PIN domain mutations on survival during infection.

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Office of the Dean, College of Sciences, Old Dominion University, Norfolk, VA, USA

Toxin-antitoxin (TA) gene pairs have been identified in nearly all bacterial genomes sequenced to date and are thought to facilitate persistence and antibiotic tolerance. TA loci are classified into various types based upon the characteristics of their antitoxins, with those in type II expressing proteic antitoxins. Many toxins from type II modules are ribonucleases that maintain a PilT N-terminus (PIN) domain containing conserved amino acids considered essential for activity. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00026-19
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http://dx.doi.org/10.1128/JB.00026-19DOI Listing
April 2019
4 Reads

Cas4 nucleases can effect specific integration of CRISPR spacers.

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, P.R. China

CRISPR-Cas systems incorporate short DNA fragments from invasive genetic elements into host CRISPR arrays in order to generate host immunity. Recently, we demonstrated that the Csa3a regulator protein triggers CCN PAM-dependent CRISPR spacer acquisition in the subtype I-A CRISPR-Cas system of However, the mechanisms underlying specific protospacer selection and spacer insertion remained unclear. Here, we demonstrate that two Cas4 family proteins (Cas4 and Csa1) have essential roles (a) in recognizing the 5' PAM and 3' nucleotide motif of protospacers and (b) in determining both the spacer length and its orientation. Read More

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http://dx.doi.org/10.1128/JB.00747-18DOI Listing
April 2019
1 Read
2.808 Impact Factor

periplasmic protein EipB is a molecular determinant of cell envelope integrity and virulence.

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA.

The Gram-negative cell envelope is a remarkable structure with core components that include an inner membrane, an outer membrane, and a peptidoglycan layer in the periplasmic space between. Multiple molecular systems function to maintain integrity of this essential barrier between the interior of the cell and its surrounding environment. We show that a conserved DUF1849-family protein, EipB, is secreted to the periplasmic space of , a monophyletic group of intracellular pathogens. Read More

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http://dx.doi.org/10.1128/JB.00134-19DOI Listing
April 2019
3 Reads

Does the semi-conservative nature of DNA replication facilitate coherent phenotypic diversity?

Authors:
Vic Norris

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Laboratory of Microbiology Signals and Microenvironment, University of Rouen, 76821 Mont Saint Aignan, France

It has been clear for over sixty years that the principal method whereby cells replicate and segregate their DNA is semi-conservative. It is much less clear as to why it should be like this rather than, say, conservative. Recently, evidence has accumulated that supports the hypothesis that one of the functions of the cell cycle is to generate phenotypically different daughter cells, even in non-differentiating bacteria such as Evidence has also accumulated that the bacterial phenotype is determined by the functioning of extended assemblies of macromolecules termed hyperstructures. Read More

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http://dx.doi.org/10.1128/JB.00119-19DOI Listing
April 2019
2 Reads

That's the way you do it.

Authors:
R Martin Roop

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834.

About 1/3 of the proteins encoded by the bacterial genomes that have been sequenced to date are proteins of 'unknown function'. Studies aimed at defining the biological functions of these proteins represent an important frontier in prokaryotic biology. The studies described by Herrou et al. Read More

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http://dx.doi.org/10.1128/JB.00216-19DOI Listing

Biochemical characterization of the methylmercaptopropionate:cob(I)alamin methyltransferase from .

J Bacteriol 2019 Apr 1. Epub 2019 Apr 1.

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801

Methanogenesis from methylated substrates is initiated by substrate specific methyltransferases that generate the central metabolic intermediate methyl-coenzyme M. This reaction involves a methyl-corrinoid protein intermediate and one or two cognate methyltransferases. Based on genetic data, the MtpC (corrinoid protein) and MtpA (methyltransferase) proteins were suggested to catalyze the methylmercaptopropionate(MMPA):Coenzyme M (CoM) methyl transfer reaction without a second methyltransferase. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00130-19
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http://dx.doi.org/10.1128/JB.00130-19DOI Listing
April 2019
2 Reads

Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis.

J Bacteriol 2019 Mar 25. Epub 2019 Mar 25.

Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie de la Méditerranée (IMM), Aix-Marseille Université - CNRS, UMR 7255, 31 chemin Joseph Aiguier CS7071, 13402 Marseille Cedex 09, France

The Type VI secretion system (T6SS) is an injection apparatus that uses a spring-like mechanism for effector delivery. The contractile tail is composed of a needle tipped by a sharpened spike and wrapped by the sheath that polymerizes in an extended conformation on the assembly platform or baseplate. Contraction of the sheath propels the needle and effectors associated with it into target cells. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00173-19
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http://dx.doi.org/10.1128/JB.00173-19DOI Listing
March 2019
4 Reads

The importance of being an antagonist as well as persistent.

J Bacteriol 2019 Mar 25. Epub 2019 Mar 25.

Center for Molecular Microbiology and Department of Oral Biology, University of Florida, Gainesville, Florida, USA.

The current work by Jain and Darveau (S. Jain and R. Darveau, J Bacteriol 00000000-00, 2019) reports the cloning and phenotypic characterization of the lipid A deacylase enzyme of Attempts to clone and thus provide proof of the existence of this enzyme had gone on for 2 decades. Read More

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http://dx.doi.org/10.1128/JB.00146-19DOI Listing

Role of , a previously uncharacterized gene, in biofilm development and antimicrobial resistance.

J Bacteriol 2019 Mar 25. Epub 2019 Mar 25.

Department of Microbiology and Immunology, University of Minnesota, Minneapolis MN

is a commensal of the human gastrointestinal tract; it is also an opportunistic pathogen and one of the leading causes of hospital-acquired infections. produces biofilms that are highly resistant to antibiotics and it has been previously reported that certain genes of the operon contribute to biofilm-associated antibiotic resistance. Despite several studies examining the operon, many gene products of this operon remain annotated as hypothetical proteins. Read More

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http://dx.doi.org/10.1128/JB.00078-19DOI Listing

Refining the pneumococcal competence regulon by RNA-sequencing.

J Bacteriol 2019 Mar 18. Epub 2019 Mar 18.

Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Nijenborgh 7, 9747 AG Groningen, the Netherlands.

Competence for genetic transformation allows the opportunistic human pathogen to take up exogenous DNA for incorporation into its own genome. This ability may account for the extraordinary genomic plasticity of this bacterium, leading to antigenic variation, vaccine escape, and the spread of antibiotic resistance. The competence system has been thoroughly studied and its regulation is well-understood. Read More

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http://dx.doi.org/10.1128/JB.00780-18DOI Listing
March 2019
3 Reads

The Yin and Yang of Lung Infections in Cystic Fibrosis: A Model for Studying Polymicrobial Interactions.

J Bacteriol 2019 Mar 18. Epub 2019 Mar 18.

Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH 03733

The streptococci are increasingly recognized as a core component of the cystic fibrosis (CF) lung microbiome, yet the role that they play in CF lung disease is unclear. The presence of group (SMG, also known as the group streptococci, AGS) correlates with exacerbation when these microbes are the predominant species in the lung. In contrast, microbiome studies have indicated that increased relative abundance of streptococci in the lung, including members of the oral microflora, correlates with less severe impacts on lung disease compared to other CF-associated microflora, indicating a complex role for this genus in the context of CF. Read More

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http://dx.doi.org/10.1128/JB.00115-19DOI Listing
March 2019
1 Read

Identification of a Formate-Dependent Uric Acid Degradation Pathway in .

J Bacteriol 2019 Mar 18. Epub 2019 Mar 18.

Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Tokyo 192-0397, Japan

Purine is a nitrogen-containing compound that is abundant in nature. In organisms that utilize purine as a nitrogen source, purine is converted to uric acid, which is then converted to allantoin. Allantoin is then converted to ammonia. Read More

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http://dx.doi.org/10.1128/JB.00573-18DOI Listing

The RNase YbeY is vital for ribosome maturation, stress resistance and virulence of the natural genetic engineer .

J Bacteriol 2019 Mar 18. Epub 2019 Mar 18.

Microbial Biology, Ruhr University Bochum, Bochum, Germany

Riboregulation involving regulatory RNAs, RNA chaperones and ribonucleases is fundamental for the rapid adaptation of gene expression to changing environmental conditions. The gene coding for the RNase YbeY belongs to the minimal prokaryotic genome set and has a profound impact on physiology in a wide range of bacteria. Here, we show that the gene is not essential. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00730-18
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http://dx.doi.org/10.1128/JB.00730-18DOI Listing
March 2019
6 Reads

Extragenic suppression of mutant phenotypes in .

J Bacteriol 2019 Mar 18. Epub 2019 Mar 18.

Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, Pennsylvania, USA

Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be post-translationally modified for full functionality; in Enterobacteria this is accomplished by two enzymes, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a conserved lysine position. Mutations to or its modifying enzymes produce pleiotropic phenotypes, including decreases in virulence, swimming motility, and extracellular polysaccharide production, as well as proteomic perturbations. Read More

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http://dx.doi.org/10.1128/JB.00722-18DOI Listing

σ inhibits PNAG exopolysaccharide synthesis and biofilm formation in .

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Laboratory of Microbial Pathogenesis, Navarrabiomed-Universidad Pública de Navarra (UPNA)-Complejo Hospitalario de Navarra (CHN), IdiSNA, Irunlarrea 3. Pamplona-31008, Navarra, Spain

clinical strains are able to produce at least two distinct types of biofilm matrixes: biofilm matrixes made of PIA/PNAG exopolysaccharide whose synthesis is mediated by the locus, and biofilm matrixes built of proteins (polysaccharide-independent). σ is a conserved alternative sigma factor that regulates the expression of more than a hundred genes in response to changes in environmental conditions. While numerous studies agree that σ is required for polysaccharide-independent biofilms, a controversy persists over the role of σ in the regulation of PIA/PNAG dependent biofilm development. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00098-19
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http://dx.doi.org/10.1128/JB.00098-19DOI Listing
March 2019
7 Reads

Cell shape and population migration are distinct steps of swarming that are decoupled on high-percentage agar.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge MA, 02138, USA

Swarming on rigid surfaces requires movement of cells as individuals and as a group of cells. For the bacterium , an individual cell can respond to a rigid surface by elongating and migrating over micron-scale distances. Cells can form groups of transiently aligned cells, and the collective population is capable of migrating over centimeter-scale distances. Read More

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http://dx.doi.org/10.1128/JB.00726-18DOI Listing

Blue light is a universal signal for chemoreceptors.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Department of Physics, Department of Chemistry, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign.

Blue light has been shown to elicit a tumbling response in , a non-phototrophic bacterium. The exact mechanism of this phototactic response is still unknown. Here, we quantify phototaxis in by analyzing single-cell trajectories in populations of free-swimming bacteria before and after light exposure. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00762-18
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http://dx.doi.org/10.1128/JB.00762-18DOI Listing
March 2019
5 Reads

Activity of Spore-Specific Respiratory Nitrate Reductase 1 of A3(2) Requires a Functional Cytochrome Oxidase Supercomplex.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Institute of Microbiology, Martin-Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3, 06120 Halle (Saale), Germany

Spores have strongly reduced metabolic activity and are produced during the complex developmental cycle of the actinobacterium Resting spores can remain viable for decades, yet little is known about how they conserve energy. It is known, however, that they can reduce either oxygen or nitrate using endogenous electron sources. uses either a cytochrome oxidase or a cytochrome - oxidase supercomplex to reduce oxygen, while nitrate is reduced by Nar-type nitrate reductases, which typically oxidize quinol directly. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00104-19
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http://dx.doi.org/10.1128/JB.00104-19DOI Listing
March 2019
4 Reads

The antibiotic korormicin A kills bacteria by producing reactive oxygen species.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

From the Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, 12180 USA

Korormicin is an antibiotic, produced by some , which selectively kills gram negative bacteria that express the Na-pumping NADH:quinone oxidoreductase (Na-NQR.) We show that, although korormicin is an inhibitor of Na-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Instead, perturbation of electron transfer inside the enzyme promotes a reaction between O and one or more redox cofactors in the enzyme (likely the FAD and 2Fe-2S center) leading to the production of reactive oxygen species (ROS). Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00718-18
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http://dx.doi.org/10.1128/JB.00718-18DOI Listing
March 2019
5 Reads

Fine tuning of σ activation suppresses multiple assembly-defective mutations in .

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Department of Molecular Biology, Princeton University, Princeton, NJ, 08540

The Gram-negative outer membrane (OM) is a selectively permeable asymmetric bilayer that allows vital nutrients to diffuse into the cell but prevents toxins and hydrophobic molecules from entering. Functionally and structurally diverse β-barrel outer membrane proteins (OMPs) build and maintain the permeability barrier, making the assembly of OMPs crucial for cell viability. In this work, we characterize an assembly-defective mutant of the maltoporin LamB, We show that the folding defect of LamB results in an accumulation of unfolded substrate that is toxic to the cell when the periplasmic protease DegP is removed. Read More

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http://dx.doi.org/10.1128/JB.00745-18DOI Listing

Suppressor mutations in overcome the acute temperature-sensitive phenotype of Δ and Δ Δ- mutants of .

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

School of Life Sciences, Arizona State University, Tempe AZ 85287-4501

In , the periplasmic protease DegP plays a critical role in degrading misfolded outer membrane proteins (OMPs). Consequently, mutants lacking DegP display a temperature-sensitive growth defect, presumably due to the toxic accumulation of misfolded OMPs. The Tol-Pal complex plays a poorly defined but an important role in envelope biogenesis, since mutants defective in this complex display a classical periplasmic leakage phenotype. Read More

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http://dx.doi.org/10.1128/JB.00742-18DOI Listing

Reciprocal regulation of PASTA kinase signaling by differential modification.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Department of Microbiology and Immunology, Center for Infectious Disease Research, Medical College of Wisconsin, Milwaukee, WI 53226

Transmembrane Ser/Thr kinases containing extracellular PASTA (BP nd er/hr-ssociated) domains are ubiquitous among and Such PASTA kinases regulate critical bacterial processes including antibiotic resistance, cell division, cell envelope homeostasis, virulence, and are sometimes essential for viability. Previous studies of purified PASTA kinase fragments revealed they are capable of autophosphorylation , typically at multiple sites on the kinase domain. Autophosphorylation of a specific structural element of the kinase known as the activation loop is thought to enhance kinase activity in response to stimuli. Read More

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http://dx.doi.org/10.1128/JB.00016-19DOI Listing
March 2019
1 Read
2.808 Impact Factor

Characterization of the phage shock protein response.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Department of Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA

The phage shock protein (Psp) system is a stress response pathway that senses and responds to inner membrane damage. The genetic components of the Psp system are present in several clinically relevant Gram-negative bacteria, including However, most of the current knowledge about the Psp response stems from studies in and In fact, the Psp response in has remained completely uncharacterized. In this study, we demonstrate that does have a functional Psp response system. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00761-18
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http://dx.doi.org/10.1128/JB.00761-18DOI Listing
March 2019
2 Reads

Onwards and [K]upwards: a new potassium importer under the spell of cyclic-di-AMP.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland, Australia

Cyclic diadenosine monophosphate (c-di-AMP) is a second messenger which plays a major role in osmotic homeostasis in bacteria. In the work of Quintana et al. (J Bacteriol DOI: 10. Read More

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http://dx.doi.org/10.1128/JB.00150-19DOI Listing
March 2019
1 Read

Entry exclusion of conjugative plasmids of the IncA, IncC and related untyped incompatibility groups.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Département de biologie, Université de Sherbrooke, Sherbrooke, Quebec, Canada

Conjugative plasmids of the IncC group, formerly known as A/C, disseminate antibiotic resistance genes globally in diverse pathogenic species of Gammaproteobacteria. genomic island 1 (SGI1) can be mobilized by IncC plasmids and was recently shown to reshape the conjugative type IV secretion system (T4SS) encoded by these plasmids to evade entry exclusion. Entry exclusion blocks DNA translocation between cells containing identical or highly similar plasmids. Read More

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http://dx.doi.org/10.1128/JB.00731-18DOI Listing

Comparative analysis of ionic strength tolerance between freshwater and marine Caulobacterales adhesins.

J Bacteriol 2019 Mar 11. Epub 2019 Mar 11.

Department of Biology, Indiana University, 1001 E. 3 St. Bloomington, Indiana 47405, USA

Bacterial adhesion is affected by environmental factors such as ionic strength, pH, temperature, and shear forces. Therefore, marine bacteria must have developed adhesins with different composition and structures than their freshwater counterparts to adapt to their natural environment. The dimorphic α-proteobacterium is a marine budding bacterium in the Caulobacterales clade. Read More

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http://dx.doi.org/10.1128/JB.00061-19DOI Listing

A new ESX-1 substrate in that is required for hemolysis but not host cell lysis.

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

Department of Biological Sciences University of Notre Dame, Notre Dame, Indiana

The ESX-1 (ESAT-6 system-1) secretion system plays a conserved role in the virulence of diverse mycobacterial pathogens including the human pathogen, and an environmental mycobacterial species. The ESX-1 system promotes the secretion of protein virulence factors to the extra-cytoplasmic environment. The secretion of these proteins triggers the host response by lysing the phagosome during macrophage infection. Read More

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http://dx.doi.org/10.1128/JB.00760-18DOI Listing
March 2019
3 Reads

The lipoprotein NlpE is a Cpx sensor that serves as a sentinel for protein sorting and folding defects in the envelope.

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

de Duve Institute, Université catholique de Louvain, Brussels, Belgium

The envelope of Gram-negative bacteria is a complex compartment that is essential for viability. To ensure survival of the bacterial cell in fluctuating environments, several signal transduction systems called envelope stress response systems (ESRS) exist to monitor envelope biogenesis and homeostasis. The Cpx two-component system is an extensively studied ESRS in that is active during exposure to a vast array of stresses and protects the envelope under these harmful circumstances. Read More

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http://dx.doi.org/10.1128/JB.00611-18DOI Listing
March 2019
2 Reads

A putative type V pilus contributes to biofilm formation capacity.

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

Institut Pasteur, Genetics of Biofilms Laboratory. 25-28 rue du Docteur Roux, 75015 Paris,cedex 15, France.

is a prominent anaerobe member of the healthy human gut microbiota. While the majority of functional studies on addressed its impact on the immune system and the utilization of diet polysaccharide, biofilm capacity and its contribution to intestinal colonization are still poorly characterized. We tested the natural adhesion of 34 isolates and showed that, although biofilm capacity is widespread among strains, this phenotype is masked or repressed in the widely used reference strain VPI 5482. Read More

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http://dx.doi.org/10.1128/JB.00650-18DOI Listing

Decreased Effective Macromolecular Crowding in Adapted to Hyperosmotic Stress.

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands

adapts to changing environmental osmolality to survive and maintain growth. It has been shown that GFP diffusion in cells adapted to osmotic upshifts is higher than expected from the increase in biopolymer volume fraction. To better understand the physicochemical state of the cytoplasm in adapted cells, we now follow the macromolecular crowding during adaptation with FRET-based sensors. Read More

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http://dx.doi.org/10.1128/JB.00708-18DOI Listing

Ethanolamine Utilization and Bacterial Microcompartment (BMC) Formation are Subject to Carbon Catabolite Repression (CCR).

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, Texas 77030.

Ethanolamine is a compound prevalent in the gastrointestinal tract that can be used as a carbon, nitrogen and/or energy source. a GI commensal and opportunistic pathogen, contains about 20 thanolamine ilization () genes encoding the necessary regulatory, enzymatic and structural proteins for this process. Here, using a chemically defined medium, two regulatory factors that affect EA utilization were examined. Read More

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http://dx.doi.org/10.1128/JB.00703-18DOI Listing

Flagellar mutants have reduced pilus synthesis in .

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

Department of Biology, Indiana University, 1001 E. 3 Street, Bloomington, IN

Surface appendages such as flagella and type IV pili mediate a broad range of bacterial behaviors including motility, attachment, and surface sensing. While many species harbor both flagella and type IV pili, little is known about how or if their synthesis is coupled. Here, we show that deletions of genes encoding different flagellum machinery components result in a reduction of pilus synthesis in First, we show that different flagellar mutants exhibit different levels of sensitivity to a pilus-dependent phage and that fewer cells within populations of flagellar mutants make pili. Read More

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http://dx.doi.org/10.1128/JB.00031-19DOI Listing