52,765 results match your criteria Journal of Bacteriology[Journal]


A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in .

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA

Here we introduce plasmids for xylose-regulated expression and repression of genes in The xylose-inducible expression vector allows for ∼100-fold induction of an reporter gene. Induction is titratable and uniform from cell-to-cell. The gene repression plasmid is a CRISPR-interference (CRISPRi) system based on a nuclease-defective, codon-optimized allele of the Cas9 protein () that is targeted to a gene of interest by a constitutively-expressed single guide RNA (sgRNA). Read More

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http://dx.doi.org/10.1128/JB.00711-18DOI Listing
February 2019

The KupA and KupB proteins of IL1403 are novel c-di-AMP receptor proteins responsible for potassium uptake.

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Department of General Microbiology, GZMB, Georg-August-Universität, Göttingen, Germany

Cyclic di-adenosine monophosphate (c-di-AMP) is a second messenger involved in diverse metabolic processes including osmolyte uptake, cell wall homeostasis, as well as antibiotic and heat resistance. In , a lactic acid bacterium which is used in the dairy industry and as a cell factory in biotechnological processes, the only reported interaction partners of c-di-AMP are the pyruvate carboxylase and BusR, the transcription regulator of the operon for glycine betaine uptake. However, recent studies uncovered a major role of c-di-AMP in the control of potassium homeostasis, and potassium as the signal that triggers c-di-AMP synthesis. Read More

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http://dx.doi.org/10.1128/JB.00028-19DOI Listing
February 2019

Inorganic polyphosphate accumulation in is regulated by DksA but not by (p)ppGpp.

Authors:
Michael J Gray

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Department of Microbiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294

Production of inorganic polyphosphate (polyP) by bacteria is triggered by a variety of different stress conditions. PolyP is required for stress survival and virulence in diverse pathogenic microbes. Previous studies have hypothesized a model for regulation of polyP synthesis in which production of the stringent response second messenger (p)ppGpp directly stimulates polyP accumulation. Read More

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http://dx.doi.org/10.1128/JB.00664-18DOI Listing
February 2019

Thymineless death in is unaffected by the chromosomal replication complexity.

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Thymineless death (TLD) is a rapid loss of viability of unclear mechanism in cultures of mutants starved for thymine/thymidine (T-starvation). It is accepted that T-starvation repeatedly breaks replication forks, while recombinational repair restores them, - but when the resulting futile breakage-repair cycle affects the small replication bubbles at , the origin is degraded, killing the cell. Indeed, cells with increased chromosomal replication complexity (CRC), expressed as elevated ori/ter ratio, die deeper during TLD. Read More

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http://dx.doi.org/10.1128/JB.00797-18DOI Listing
February 2019

Calcium ion modulates amino acid sensing of the chemoreceptor Mlp24 of .

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

Bacteria sense environmental chemicals using chemosensor proteins, most of which are present in the cytoplasmic membrane. Canonical chemoreceptors bind their specific ligands in their periplasmic domain, and the ligand binding creates a molecular stimulus that is transmitted into the cytoplasm, leading to various cellular responses, such as chemotaxis and specific gene expression. , the causative agent of cholera, contains about 44 putative sensor proteins, which are homologous to methyl-accepting chemotaxis proteins involved in chemotaxis. Read More

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http://dx.doi.org/10.1128/JB.00779-18DOI Listing
February 2019

Resistance to two vinylglycine antibiotic analogs is conferred by inactivation of two separate amino acid transporters in .

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, Saskatchewan, Canada, S4S0A2

is the causal agent of fire blight of apple and pear trees. Several bacteria have been shown to produce antibiotics that antagonize including pantocins, herbicolins, dapdiamides, and the vinylglycines, 4-formylaminooxyvinylglycine (FVG) and 4-aminoethoxyvinylglycine (AVG). BRT175 was previously shown to exhibit antibiotic activity against via the production of Natural Product 1 (PNP-1), later shown to be FVG; however, exposure of to FVG results in spontaneously resistant mutants. Read More

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http://dx.doi.org/10.1128/JB.00658-18DOI Listing
February 2019

Defining the role of the Sht-family proteins in zinc acquisition and complement evasion.

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

ISP, Université de Tours, INRA, UMR1282, F-37032 Tours, France

is part of the human intestinal and uro-genital microbiota, but also a leading cause of septicemia and meningitis in neonates. Its ability to cause disease depends upon the acquisition of nutrients from its environment, including the transition metal ion zinc. The primary zinc acquisition system of the pathogen is the Adc/Lmb ABC permease, which is essential for viability in zinc-restricted environments. Read More

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http://dx.doi.org/10.1128/JB.00757-18DOI Listing
February 2019

Transfer RNA Modification Profiles and Codon Decoding Strategies in .

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, Ohio 45221-0172, United States

Transfer RNAs (tRNAs) play a critical role in messenger RNA (mRNA) decoding, and post-transcriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and localized to particular tRNA sequences. However, far less is known regarding tRNA modifications in archaea. Read More

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http://dx.doi.org/10.1128/JB.00690-18DOI Listing
February 2019

RpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth.

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Coxiella Pathogenesis Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

, the etiological agent of Q fever, undergoes a unique biphasic developmental cycle where bacteria transition from a replicating (exponential phase) large cell variant (LCV) form to a non-replicating (stationary phase) small cell variant (SCV) form. The alternative sigma factor RpoS is an essential regulator of stress responses and stationary phase physiology in several bacterial species, including which has a developmental cycle superficially similar to that of Here, we used a Δ mutant to define the role of RpoS in intracellular growth and SCV development. Growth yields following infection of Vero epithelial cells or THP-1 macrophage-like cells with the mutant in the SCV form, but not the LCV form, were significantly lower than that of wild-type bacteria. Read More

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http://dx.doi.org/10.1128/JB.00009-19DOI Listing
February 2019

Roles of RecA, Nucleotide Excision Repair and TLS Polymerases In Counteracting Cr(VI)-Promoted DNA Damage.

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Department of Biology, Division of Natural and Exact Sciences, University of Guanajuato, Guanajuato, Gto. México

Bacteria deploy global programs of gene expression, including components of the SOS-response, to counteract the cytotoxic and genotoxic effects of environmental DNA damaging factors. Here, we report that genetic damage promoted by hexavalent chromium elicited the SOS-response in , as evidenced by the induction of transcriptional , and P - fusions. Accordingly, strains deficient in homologous recombination (RecA) and nucleotide excision repair (UvrA), components of the SOS response, were significantly more sensitive to Cr(VI) treatment than cells of the wild-type strain. Read More

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http://dx.doi.org/10.1128/JB.00073-19DOI Listing
February 2019

Nitrogen regulator GlnR directly controls transcription of operon involved in methylcitrate cycle in .

J Bacteriol 2019 Feb 11. Epub 2019 Feb 11.

Lab of Biosystems and Microanalysis, Biomedical Nanotechnology Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

utilizes fatty acids of the host as the carbon source. Metabolism of odd chain fatty acids by produces propionyl-CoA. Methylcitrate cycle is essential for Mycobacteria to utilize the propionyl-CoA to persist and grow on these fatty acids. Read More

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http://dx.doi.org/10.1128/JB.00099-19DOI Listing
February 2019
1 Read

Inactivation of inhibits biofilm formation by activating the disulfide stress regulator Spx in .

Authors:
Kazuo Kobayashi

J Bacteriol 2019 Feb 4. Epub 2019 Feb 4.

Division of Biological Science, Nara Institute of Science & Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan

forms biofilms in response to internal and external stimuli. We previously showed that the deletion mutant was defective in biofilm formation but the reason for this remains unidentified. CysL is a transcriptional activator of the operon, which encodes sulfite reductase, an enzyme involved in cysteine biosynthesis. Read More

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http://dx.doi.org/10.1128/JB.00712-18DOI Listing
February 2019
1 Read

Can Inhibit Growth of Streptococcal Species via Siderophore Production.

J Bacteriol 2019 Feb 4. Epub 2019 Feb 4.

Department of Microbiology and Immunology, v, Hanover, NH 03755

Cystic Fibrosis (CF) is a genetic disease that causes patients to accumulate thick, dehydrated mucus in the lung and develop chronic, polymicrobial infections due to reduced mucociliary clearance. These chronic polymicrobial infections and subsequent decline in lung function are significant factors in the morbidity and mortality of CF. and spp. Read More

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http://dx.doi.org/10.1128/JB.00014-19DOI Listing
February 2019
1 Read

One Basic Blueprint, Many Different Motors.

Authors:
Michael D Manson

J Bacteriol 2019 Feb 4. Epub 2019 Feb 4.

Department of Biology, Texas A&M University, College Station, TX 77843-3258

The cytoplasmic C-ring of the bacterial flagellum is known as the switch complex. It binds the response regulator phospho-CheY to control the direction of flagellar rotation. The C-ring of enteric bacteria is well characterized. Read More

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http://dx.doi.org/10.1128/JB.00019-19DOI Listing
February 2019
1 Read

SlyA and HilD counteract H-NS-mediated repression on the virulence operon of Typhimurium and thus promote its activation by OmpR.

J Bacteriol 2019 Feb 4. Epub 2019 Feb 4.

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

H-NS-mediated repression of acquired genes and the subsequent adaptation of regulatory mechanisms that counteract this repression have played a central role in the pathogenicity evolution. The pathogenicity island 2 (SPI-2) is an acquired chromosomal region containing genes necessary for to colonize and replicate in different niches of hosts. The operon, located in SPI-2, encodes the two-component system SsrA-SsrB, which positively controls the expression of the SPI-2 genes, but also other many genes located outside SPI-2. Read More

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http://dx.doi.org/10.1128/JB.00530-18DOI Listing
February 2019

Identification of a fifth antibacterial toxin produced by a single strain.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Bacteroidales are the most abundant Gram-negative bacteria of the healthy human colonic microbiota, in many individuals comprising nearly 50% of their colonic bacteria. Numerous species and strains of gut Bacteroidales are present simultaneously at high concentration in this ecosystem. Studies are revealing that gut have numerous antibacterial weapons to antagonize closely related members. Read More

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http://dx.doi.org/10.1128/JB.00577-18DOI Listing
January 2019
1 Read

Experimental evolution of extreme resistance to ionizing radiation in after 50 cycles of selection.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Department of Biochemistry, University of Wisconsin - Madison. Madison, WI 53706-1544

In previous work (1, 2), we demonstrated that could acquire substantial levels of resistance to ionizing radiation (IR) via directed evolution. Major phenotypic contributions involved adaptation of organic systems for DNA repair. We have now undertaken an extended effort to generate populations that are as resistant to IR as After an initial 50 cycles of selection using high-energy electron beam IR, four replicate populations exhibit major increases in IR resistance, but have not yet reached IR resistance equivalent to Regular deep sequencing reveals complex evolutionary patterns with abundant clonal interference. Read More

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http://dx.doi.org/10.1128/JB.00784-18DOI Listing
January 2019

Light modulates the physiology of non-phototrophic Actinobacteria.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

University of Delaware, Department of Civil and Environmental Engineering, University of Delaware, Center for Bioinformatics and Computational Biology, University of Delaware, Sequencing and Genotyping Center;University of Delaware, Delaware Biotechnology Institute; University of Delaware, Department of Biology; University of Innsbruck, Research Department for Limnology, Mondsee.

Light is a source of energy and an environmental cue that is available in excess in most surface environments. In prokaryotic systems, conversion of light to energy by photoautotrophs and photoheterotrophs is well understood, but the conversion of light to information and the cellular response to that information has been characterized in only a few species. Our goal was to explore the response of freshwater Actinobacteria, which are ubiquitous in illuminated aquatic environments, to light. Read More

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http://dx.doi.org/10.1128/JB.00740-18DOI Listing
January 2019

SpoIVA-SipL complex formation is essential for spore assembly.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA

Spores are the major infectious particle of the Gram-positive nosocomial pathogen, (formerly ) , but the molecular details of how this organism forms these metabolically dormant cells remain poorly characterized. The composition of the spore coat in differs markedly from that defined in the well-studied organism, , with only 25% of the ∼70 spore coat proteins being conserved between the two organisms, and only 2 of 9 coat assembly (morphogenetic) proteins defined in having homologs in We previously identified SipL as a clostridia-specific coat protein essential for functional spore formation. Heterologous expression analyses in revealed that SipL directly interacts with SpoIVA, a coat morphogenetic protein conserved in all spore-forming organisms, through SipL's C-terminal LysM domain. Read More

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http://dx.doi.org/10.1128/JB.00042-19DOI Listing
January 2019

Genetics behind the biosynthesis of nonulosonic acid containing lipooligosaccharides in .

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Department of Chemistry and Biology, Ryerson University, Toronto, Canada.

and are the most common cause of bacterial gastroenteritis in the world. Ganglioside mimicry by lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from the glycosyltransferase (GT) family 42 are essential for the expression of ganglioside mimics in Recently, two novel GT-42 genes, and , have been identified in Despite being present in ∼11% of currently available genomes, the biological role of and is unknown. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00759-18
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http://dx.doi.org/10.1128/JB.00759-18DOI Listing
January 2019
2 Reads

Roles of the DedD protein in cell constriction.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Department of Molecular Biology & Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960

Two key tasks of the bacterial septal ring (SR) machinery during cell constriction are the generation of an inward growing annulus of septal peptidoglycan (sPG) and the concomitant splitting of its outer edge into two layers of polar PG that will be inherited by the two new cell ends. FtsN is an essential SR protein that helps trigger the active constriction phase in by inducing a self-enhancing cycle of processes that includes both sPG synthesis and splitting, and that we refer to as the sPG-loop. DedD is a SR protein that resembles FtsN in several ways. Read More

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http://dx.doi.org/10.1128/JB.00698-18DOI Listing
January 2019
1 Read
2.808 Impact Factor

Mechanisms of resistance to the contact-dependent bacteriocin CdzC/D in .

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Department of Biology Howard Hughes Medical Institute Graduate Program in Microbiology Graduate Program in Biology; Massachusetts Institute of Technology, Cambridge, MA, USA

The Cdz bacteriocin system allows the aquatic oligotrophic bacterium to kill closely related species in a contact-dependent manner. The toxin, which aggregates on the surface of producer cells, is composed of two small hydrophobic proteins, CdzC and CdzD, each bearing an extended glycine-zipper motif, that together induce inner membrane depolarization and kill target cells. To further characterize the mechanism of Cdz delivery and toxicity, we screened for mutations that render a target strain resistant to Cdz-mediated killing. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00538-18
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http://dx.doi.org/10.1128/JB.00538-18DOI Listing
January 2019
6 Reads

Novel 3,6-Dihydroxypicolinic Acid Decarboxylase Mediated Picolinic Acid Catabolism in JQ135.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

Picolinic acid (PA), a typical C2-carboxylated pyridine derivative, is a metabolite of L-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Read More

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http://dx.doi.org/10.1128/JB.00665-18DOI Listing
January 2019
5 Reads

Motor properties of PilT-independent type 4 pilus retraction in gonococci.

J Bacteriol 2019 Jan 28. Epub 2019 Jan 28.

University of Cologne, Institute for Biological Physics

Bacterial type 4 pili (T4P) belong to the strongest molecular machines. The gonococcal T4P retraction ATPase PilT supports forces exceeding 100 pN during T4P retraction. Here, we address the question whether gonococcal T4P retract in the absence of PilT. Read More

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http://dx.doi.org/10.1128/JB.00778-18DOI Listing
January 2019

Characterization of L-carnitine metabolism in .

J Bacteriol 2019 Jan 22. Epub 2019 Jan 22.

Génomique métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, 91057 Evry, France

L-carnitine is a trimethylammonium compound mostly known for its contribution to fatty acid transport into mitochondria. In bacteria, it is synthesized from γ-butyrobetaine (GBB) and can be used as a carbon source. L-carnitine can be formed directly by GBB hydroxylation or synthesized a biosynthetic route analogous to fatty-acid degradation. Read More

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http://dx.doi.org/10.1128/JB.00772-18DOI Listing
January 2019
1 Read

PmrC/EptA and CptA negatively affect outer membrane vesicle production in .

J Bacteriol 2019 01 22. Epub 2019 Jan 22.

Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada.

Outer membrane vesicles (OMVs) are naturally produced by Gram-negative bacteria by a bulging of the outer membrane (OM) and subsequent release in the environment. By serving as vehicles for various cargos, including proteins, nucleic acids and small metabolites, OMVs are central to interbacterial interactions and both symbiotic and pathogenic host bacterial interactions. However, despite their importance, the mechanism of OMV formation remains unclear. Read More

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http://dx.doi.org/10.1128/JB.00454-18DOI Listing
January 2019
2 Reads

RNase HIII is important for Okazaki fragment processing in .

J Bacteriol 2019 Jan 22. Epub 2019 Jan 22.

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109

RNA-DNA hybrids are common in chromosomal DNA. Persistent RNA-DNA hybrids result in replication fork stress, DNA breaks, and neurological disorders in humans. During replication, Okazaki fragment synthesis relies on frequent RNA primer placement, providing one of the most prominent forms of covalent RNA-DNA strands The mechanism of Okazaki fragment maturation, which involves RNA removal and subsequent DNA replacement, in bacteria lacking RNase HI remains unclear. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00686-18
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http://dx.doi.org/10.1128/JB.00686-18DOI Listing
January 2019
11 Reads

Characterization of the HslUV and ClpXP protease systems in free-living and symbiotic states.

J Bacteriol 2019 Jan 22. Epub 2019 Jan 22.

Molecular Plant Science Program and Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340

Symbiotic nitrogen fixation (SNF) in the interaction between the soil bacteria and legume plant is carried out in specialized root organs called nodules. During nodule development, each symbiont must drastically alter their proteins, transcripts and metabolites in order to support nitrogen fixation. Moreover, bacteria within the nodules are under stress, including challenges by plant antimicrobial peptides, low pH, limited oxygen availability, and strongly reducing conditions, all of which challenge proteome integrity. Read More

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http://dx.doi.org/10.1128/JB.00498-18DOI Listing
January 2019
1 Read

The GGDEF domain of the phosphodiesterase PdeB in mediates recruitment by the polar landmark protein HubP.

J Bacteriol 2019 Jan 22. Epub 2019 Jan 22.

Justus-Liebig Universität, Department of Microbiology and Molecular Biology, 35392 Giessen, Germany

Bacteria commonly exhibit a high degree of cellular organization and polarity, which affects many vital processes such as replication, cell division and motility. In and other bacteria, HubP is a polar marker protein, which is involved in proper chromosome segregation, placement of the chemotaxis system and various aspects of pili- and flagella-mediated motility. Here we show that HubP also recruits a transmembrane multi-domain protein, PdeB, to the flagellated cell pole. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00534-18
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http://dx.doi.org/10.1128/JB.00534-18DOI Listing
January 2019
3 Reads

Plasmid characteristics modulate the propensity of gene exchange in bacterial vesicles.

J Bacteriol 2019 Jan 22. Epub 2019 Jan 22.

University of Southern California, Department of Biological Sciences University of Southern California, Department of Physics and Astronomy, University of Southern California, Seaver Science Center (SSC) 212, 920 Bloom Walk, Los Angeles, CA 90089

Horizontal gene transfer is responsible for the exchange of many types of genetic elements, including plasmids. Properties of the exchanged genetic element are known to influence the efficiency of transfer via the mechanisms of conjugation, transduction or transformation. Recently, an alternative, general pathway of horizontal gene transfer has been identified, gene exchange by extracellular vesicles. Read More

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http://dx.doi.org/10.1128/JB.00430-18DOI Listing
January 2019

Global analysis of genes essential for growth and for fitness during competitive infection of Fischer 344 rats.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Defence Science and Technology Laboratory, Chemical, Biological and Radiological Division, Porton Down, Salisbury, SP4 0JQ, UK.

The highly virulent intracellular pathogen is a Gram negative bacterium that has a wide host range including humans and is the causative agent of tularemia. To identify new therapeutic drug targets, vaccine candidates and investigate the genetic basis of virulence in the Fischer 344 rat we have constructed an Schu S4 transposon library. This library consists of over 300,000 unique transposon mutants and represents a transposon insertion for every 6 bp of the genome. Read More

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http://dx.doi.org/10.1128/JB.00630-18DOI Listing
January 2019
4 Reads

Flagellar stators stimulate c-di-GMP production by .

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth

Flagellar motility is critical for surface attachment and biofilm formation in many bacteria. A key regulator of flagellar motility in and other microbes is cyclic diguanylate (c-di-GMP). High levels of this second messenger repress motility and stimulate biofilm formation. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00741-18
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http://dx.doi.org/10.1128/JB.00741-18DOI Listing
January 2019
2 Reads

Purification of a crenarchaeal ATP synthase in the light of the unique bioenergetics of species.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Institute for Microbiology and Archaeal Centre, Regensburg University, Regensburg, Germany.

In this study, the ATP synthase of was purified, characterized and structurally compared to the respective enzymes of the other species to shed light on energy conservation in this unique group of Archaea. The crenarchaeal genus comprises three described species: and from hot marine sediments near Iceland and from a hydrothermal vent system in the Pacific Ocean. This genus is unique among all Archaea due to the unusual cell envelope consisting of two membranes that enclose a large intermembrane compartment (IMC). Read More

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http://dx.doi.org/10.1128/JB.00510-18DOI Listing
January 2019
1 Read

Lag phase - a dynamic, organized, adaptive, and evolvable period that prepares bacteria for cell division.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Department of Chemistry, University of Manitoba, Winnipeg, Canada, R3T 2N2

Lag is a temporary period of non-replication seen in bacteria that are introduced to new media. Despite latency being described by Müller in 1895, until only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature to date on the transcriptomic, proteomic, metabolomic, physiological, biochemical, and evolutionary features of prokaryotic lag. Read More

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http://dx.doi.org/10.1128/JB.00697-18DOI Listing
January 2019

Cystic fibrosis-associated strain-specific adaptations and responses to pH.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA

The airway fluids of cystic fibrosis (CF) patients contain local pH gradients and are more acidic than that of healthy indviduals. pH is a critical factor that is often overlooked in studies seeking to recapitulate the infection microenvironment. We sought to determine the impact of pH on the physiology of a ubiqituous yet understudied microbe, Phylogenomics was first used to reconstruct evolutionary relationships between 74 strains of (59 from CF patients). Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00478-18
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http://dx.doi.org/10.1128/JB.00478-18DOI Listing
January 2019
4 Reads

Tuning the alternative sigma factor SigF through the multidomain regulator Rv1364c and osmosensory kinase, protein kinase D.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Allergy and Infectious Disease Unit, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi-110007

Bacterial alternative sigma factors are mostly regulated by a partner-switching mechanism. Regulation of the virulence associated alternative sigma factor, SigF, of , has been an area of intrigue with more predicted regulators compared to other sigma factors in this organism. Rv1364c is one such predicted regulator, the mechanism of which is confounded by the presence of both anti-sigma and anti-sigma-antagonist functions in a single polypeptide. Read More

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http://dx.doi.org/10.1128/JB.00725-18DOI Listing
January 2019

Effect of the MotA(M206I) mutation on torque generation and stator assembly in the H-driven flagellar motor.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Department of Applied Physics, Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aoba-ku, Sendai, Miyagi 980-8579, Japan

The bacterial flagellar motor is composed of a rotor and a dozen stators and converts the ion flux through the stator into torque. Each stator unit alternates in its attachment to and detachment from the rotor even during rotation. In some species, stator assembly depends on the input energy, but it remains unclear how an electrochemical potential across the membrane (e. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00727-18
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http://dx.doi.org/10.1128/JB.00727-18DOI Listing
January 2019
3 Reads

The respiratory selenite reductase from strain MLS10.

J Bacteriol 2019 Jan 14. Epub 2019 Jan 14.

Department of Biological Sciences, Duquesne University, Pittsburgh PA 15282 USA

The putative respiratory selenite [Se(IV)] reductase (Srr) from MLS10 has been identified through a polyphasic approach involving genomics, proteomics, and enzymology. Non-denaturing gel assays were used to identify Srr in cell fractions and the active band was shown to contain a single protein of 80 kDa. The protein was identified through LC-MS-MS as a homolog of the catalytic subunit of polysulfide reductase (PsrA). Read More

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http://dx.doi.org/10.1128/JB.00614-18DOI Listing
January 2019
2 Reads
2.808 Impact Factor

Topoisomerase III Acts at the Replication Fork to Remove Precatenanes.

J Bacteriol 2019 Jan 7. Epub 2019 Jan 7.

Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA

The role of DNA topoisomerase III (Topo III) in bacterial cells has proven elusive. Whereas eukaryotic Top IIIα homologs are clearly involved with homologs of the bacterial DNA helicase RecQ in unraveling double Holliday junctions, preventing crossover exchange of genetic information at unscheduled recombination intermediates, and Top IIIβ homologs have been shown to be involved in regulation of various mRNAs involved in neuronal function, little evidence for similar reactions exists in bacteria. Instead, most data points to Topo III playing a supplemental role to topoisomerase IV in unlinking daughter chromosomes during DNA replication. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00563-18
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http://dx.doi.org/10.1128/JB.00563-18DOI Listing
January 2019
2 Reads

The orphan hybrid histidine protein kinase, SinK, acts as a signal integrator to fine-tune multicellular behavior in .

J Bacteriol 2019 Jan 7. Epub 2019 Jan 7.

Department of Biological Sciences, Wayne State University, Detroit, MI, USA

His-Asp phosphorelay (a.k.a. Read More

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http://dx.doi.org/10.1128/JB.00561-18DOI Listing
January 2019

Prevention of surface-associated calcium phosphate by the two component system CvsSR.

J Bacteriol 2019 Jan 7. Epub 2019 Jan 7.

School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY

CvsSR is a Ca-induced two-component system (TCS) in the plant pathogen pv. tomato DC3000 (). Here we discovered CvsSR is induced by Fe, Zn, and Cd However, only supplementation of Ca to medium resulted in rugose, opaque colonies in Δ and Δ strains. Read More

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http://dx.doi.org/10.1128/JB.00584-18DOI Listing
January 2019

Increased intracellular cyclic-di-AMP levels sensitize subsp. to osmotic stress, and reduce biofilm formation and adherence on intestinal cells.

J Bacteriol 2019 Jan 7. Epub 2019 Jan 7.

Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore

Cyclic-di-AMP is a recently identified second messenger exploited by a number of Gram-positive bacteria to regulate important biological processes. Here, we studied the phenotypic alterations induced by increased intracellular c-di-AMP levels in , an opportunistic pathogen responsible for septicemia and endocarditis in the elderly. We report that a c-di-AMP phosphodiesterase knock-out mutant, which displays a 1. Read More

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http://jb.asm.org/lookup/doi/10.1128/JB.00597-18
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http://dx.doi.org/10.1128/JB.00597-18DOI Listing
January 2019
6 Reads

MOLECULAR MECHANISMS OF INVERSIONS IN THE LOCUS OF .

J Bacteriol 2019 Jan 7. Epub 2019 Jan 7.

Center for Infectious Disease Research, Department of Basic Medical Science, School of Medicine, Tsinghua University, Beijing 100084,China

(pneumococcus), a major human pathogen, is well known for its adaptation to various host environments. Multiple DNA inversions in the three DNA methyltransferase genes ( and ) of colony opacity determinant () locus generate extensive epigenetic and phenotypic diversity. However, it is unclear whether all three genes are functional and how the inversions mechanistically occur. Read More

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http://dx.doi.org/10.1128/JB.00581-18DOI Listing
January 2019

Selectivity among anti-σ factors by ClpX influences intracellular levels of Extracytoplasmic Function σ factors.

J Bacteriol 2019 Jan 7. Epub 2019 Jan 7.

Molecular Biophysics Unit, Indian Institute of Science, Bangalore-560012, India

Extracytoplasmic Function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. membrane associated anti-σ factors have a small stable RNA gene A-like degron for targeted proteolysis. Interaction between the unfoldase, ClpX, and the substrate with an accessible degron initiates energy-dependent proteolysis. Read More

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http://dx.doi.org/10.1128/JB.00748-18DOI Listing
January 2019

SodA contributes to the virulence of avian pathogenic O2 strain E058 in experimentally infected chickens.

J Bacteriol 2019 Jan 2. Epub 2019 Jan 2.

Animal Infectious Disease Laboratory, Ministry of Agriculture, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China

Avian pathogenic (APEC), the common pathogen of avian colibacillosis, encounter reactive oxygen species (ROS) during the infection process. Superoxide dismutases (SODs), acting as antioxidant factors, can protect against ROS-mediated host defenses. Our previous reports showed that the gene (encoding a Mn-cofactor-containing SOD [MnSOD]) is highly expressed during the septicemic infection process of APEC. Read More

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http://dx.doi.org/10.1128/JB.00625-18DOI Listing
January 2019
1 Read

Membrane Proteomes and Ion Transporters in Dormant and Germinating Spores.

J Bacteriol 2019 Jan 2. Epub 2019 Jan 2.

Department of Biological Sciences, Virginia Tech, 926 West Campus Drive, Blacksburg, VA 24061

Bacterial endospores produced by and species can remain dormant and highly resistant to environmental insults for long periods but can also rapidly germinate in response to a nutrient rich environment. Multiple proteins involved in sensing and responding to nutrient germinants, initiating solute and water transport, and accomplishing spore wall degradation are associated with the membrane surrounding the spore core. In order to more fully catalog proteins that may be involved in spore germination as well as to identify protein changes taking place during germination, unbiased proteomic analyses of membrane preparations isolated from dormant and germinated spores of and were undertaken. Read More

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http://dx.doi.org/10.1128/JB.00662-18DOI Listing
January 2019

Transcriptional regulation of by both IcaR and TcaR in .

J Bacteriol 2019 Jan 2. Epub 2019 Jan 2.

Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE. USA.

is a primary cause of biofilm-mediated infections in humans due to adherence to foreign bodies. A major staphylococcal biofilm accumulation molecule is polysaccharide intracellular adhesin (PIA), which is synthesized by enzymes encoded by the operon. Expression of PIA is highly variable amongst clinical isolates suggesting that PIA expression levels are selected in certain niches of the host. Read More

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http://dx.doi.org/10.1128/JB.00524-18DOI Listing
January 2019

Characterization of FlgP, an Essential Protein for Flagellar Assembly in .

J Bacteriol 2019 Mar 11;201(5). Epub 2019 Feb 11.

Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico

The flagellar lipoprotein FlgP has been identified in several species of bacteria, and its absence provokes different phenotypes. In this study, we show that in the alphaproteobacterium , a Δ mutant is unable to assemble the hook and the filament. In contrast, the membrane/supramembrane (MS) ring and the flagellar rod appear to be assembled. Read More

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http://dx.doi.org/10.1128/JB.00752-18DOI Listing
March 2019
2.808 Impact Factor

2018 Jack Kenney Award for Outstanding Service.

Authors:
Thomas J Silhavy

J Bacteriol 2019 Jan 7;201(1). Epub 2018 Dec 7.

Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA

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http://dx.doi.org/10.1128/JB.00617-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287461PMC
January 2019