3,877 results match your criteria Journal of Assisted Reproduction and Genetics [Journal]


Expanded genetic carrier screening in clinical practice: a current survey of patient impressions and attitudes.

J Assist Reprod Genet 2019 Feb 13. Epub 2019 Feb 13.

Department of Obstetrics and Gynecology, Boonshoft School of Medicine, Wright State University, 128 Apple Street, Suite 3800 Weber CHE, Dayton, OH, 45409, USA.

Purpose: Expanded genetic carrier screening (ECS) is an important part of gynecological practice and preconception planning. We evaluated the awareness and attitudes among women regarding ECS and factors that may influence decision-making in a family planning context.

Methods: A 32-question survey in an academic university practice was given to 521 women who were either currently pregnant (n = 108), undergoing gynecologic care who were considering future fertility (n = 308), and considering or receiving fertility treatment (n = 105). Read More

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http://dx.doi.org/10.1007/s10815-019-01414-zDOI Listing
February 2019

Preimplantation genetic testing using Karyomapping for a paternally inherited reciprocal translocation: a case study.

J Assist Reprod Genet 2019 Feb 12. Epub 2019 Feb 12.

Monash IVF, Suite 1, 252 Clayton Rd, Clayton, Victoria, 3168, Australia.

Purpose: Preimplantation genetic testing (PGT) using Karyomapping is used to screen embryos for single gene disorders prior to implantation. While Karyomapping is not designed to screen for abnormalities in chromosome copy number, this testing is based upon a genome-wide analysis of single nucleotide polymorphisms (SNP) and, as such, some chromosome abnormalities are detected. The aim of this study was to validate whether Karyomapping could provide reliable and accurate PGT for a paternal 46,XY,t(10;19)(p15;p13. Read More

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http://dx.doi.org/10.1007/s10815-019-01413-0DOI Listing
February 2019

Maternal age, history of miscarriage, and embryonic/fetal size are associated with cytogenetic results of spontaneous early miscarriages.

J Assist Reprod Genet 2019 Feb 9. Epub 2019 Feb 9.

Center for Maternal-Fetal, Neonatal and Reproductive Medicine, National Center for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.

Purpose: To clarify the associations of the maternal age, history of miscarriage, and embryonic/fetal size at miscarriage with the frequencies and profiles of cytogenetic abnormalities detected in spontaneous early miscarriages.

Methods: Miscarriages before 12 weeks of gestation, whose karyotypes were evaluated by G-banding between May 1, 2005, and May 31, 2017, were included in this study. The relationships between their karyotypes and clinical findings were assessed using trend or chi-square/Fisher's exact tests and multivariate logistic analyses. Read More

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http://dx.doi.org/10.1007/s10815-019-01415-yDOI Listing
February 2019

Human β-defensin 1 in follicular fluid and semen: impact on fertility.

J Assist Reprod Genet 2019 Feb 2. Epub 2019 Feb 2.

Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy.

Purpose: β-defensins are antimicrobial peptides expressed at mucosal level of male and female genito-urinary tract, where they exert protective functions against infections, possibly preserving human health and fertility. In our study, we investigated the possible involvement of β-defensins in female and male infertility in Italian infertile couples (i) evaluating the presence of human β-defensin 1 (hBD-1) in follicular fluid (FF) and its correlation with in vitro fertilization (IVF) outcomes; (ii) investigating the relationship between hBD-1 levels in semen and IVF outcomes (comprising correlation with sperm parameters); and (iii) exploring the effect of hBD-1 peptide on spermatozoa motility in vitro.

Methods: A perspective observational analytic pilot study was conducted. Read More

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http://dx.doi.org/10.1007/s10815-019-01409-wDOI Listing
February 2019
1 Read

An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploidy.

J Assist Reprod Genet 2019 Feb 1. Epub 2019 Feb 1.

DAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy.

Purpose: To investigate the association of cumulus cell (CC)-related expression of a selected cluster of key genes (PTGS2, CAMK1D, HAS2, STC1, and EFNB2) with embryo development to blastocyst.

Methods: Exploratory study at a private clinic. Eighteen advanced maternal age patients were enrolled (37. Read More

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http://dx.doi.org/10.1007/s10815-019-01410-3DOI Listing
February 2019

Artificial intelligence and machine learning for human reproduction and embryology presented at ASRM and ESHRE 2018.

J Assist Reprod Genet 2019 Jan 28. Epub 2019 Jan 28.

Department of Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Sixteen artificial intelligence (AI) and machine learning (ML) approaches were reported at the 2018 annual congresses of the American Society for Reproductive Biology (9) and European Society for Human Reproduction and Embryology (7). Nearly every aspect of patient care was investigated, including sperm morphology, sperm identification, identification of empty or oocyte containing follicles, predicting embryo cell stages, predicting blastocyst formation from oocytes, assessing human blastocyst quality, predicting live birth from blastocysts, improving embryo selection, and for developing optimal IVF stimulation protocols. This represents a substantial increase in reports over 2017, where just one abstract each was reported at ASRM (AI) and ESHRE (ML). Read More

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http://link.springer.com/10.1007/s10815-019-01408-x
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http://dx.doi.org/10.1007/s10815-019-01408-xDOI Listing
January 2019
5 Reads

Ethnical and sociocultural differences causing infertility are poorly understood-insights from the Arabian perspective.

J Assist Reprod Genet 2019 Jan 26. Epub 2019 Jan 26.

IVIRMA Middle-East Fertility Clinic, Marina Village, Villa B 22, POB 60202, Abu Dhabi, United Arab Emirates.

Infertility is acknowledged worldwide as a major health concern. Although global levels of primary and secondary infertility have hardly changed between 1990 and 2010, significant regional differences have been reported. The prevalence of infertility in women has been estimated to be one in every seven couples in the western world and one in every four couples in developing countries. Read More

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http://dx.doi.org/10.1007/s10815-019-01411-2DOI Listing
January 2019
1 Read

Obstetrical complications of thin endometrium in assisted reproductive technologies: a systematic review.

J Assist Reprod Genet 2019 Jan 25. Epub 2019 Jan 25.

Virginia Center for Reproductive Medicine, Reston, VA, USA.

Purpose: The aim of the systematic review is to describe the obstetrical complications associated with pregnancies in the context of a thin endometrial lining.

Methods: Systematic review of the literature.

Results: Patients who conceive in the setting of a thin endometrium have a significantly increased risk of early pregnancy loss, namely miscarriage and ectopic pregnancy. Read More

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http://dx.doi.org/10.1007/s10815-019-01407-yDOI Listing
January 2019
2 Reads

The interaction effect of angiogenesis and endothelial dysfunction-related gene variants increases the susceptibility of recurrent pregnancy loss.

J Assist Reprod Genet 2019 Jan 24. Epub 2019 Jan 24.

Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

Purpose: The role of genetic polymorphisms in the pathogenesis of recurrent pregnancy loss (RPL) has been studied intensively. Complex diseases, including miscarriage, are believed to have a polygenic basis, and gene-gene interactions can play a significant role in the etiology of the disease. This study was conducted to investigate the association of gene-gene interactions with angiogenesis, endothelial dysfunction-related gene polymorphisms, and RPL. Read More

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http://dx.doi.org/10.1007/s10815-019-01403-2DOI Listing
January 2019

Unbalanced X;9 translocation in an infertile male with de novo duplication Xp22.31p22.33.

J Assist Reprod Genet 2019 Jan 24. Epub 2019 Jan 24.

Laboratory of Genetics, Center of Experimental Medicine and Translational Research, Biomedical Research Foundation of the Academy of Athens, (BRFAA), Athens, Greece.

Purpose: Male carriers of an X-autosome translocation are generally infertile, regardless of the position of the breakpoint on the X chromosome while the pathogenicity of Xp22.3 subtelomeric duplications is under debate. To shed light into this controversy, we present a rare case, of an azoospermic male with no other significant clinical findings, in whom classical cytogenetics revealed additional unbalanced chromosomal material, at the telomere of the long arm of one homolog of chromosome 9. Read More

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http://dx.doi.org/10.1007/s10815-019-01405-0DOI Listing
January 2019
1 Read

A comparison of morphokinetic markers predicting blastocyst formation and implantation potential from two large clinical data sets.

J Assist Reprod Genet 2019 Jan 22. Epub 2019 Jan 22.

IVIRMA Valencia, IVF, Valencia, Spain.

Purpose: To demonstrate whether the standard morphokinetic markers used for embryo selection have a similar relationship to blastocyst formation and implantation in two large clinical data sets.

Methods: This is a retrospective cohort analysis striving to answer two distinct questions utilizing data sets from two large IVF clinics. Blastocysts (BL) and implanted blastocysts (I) in both clinics, IVI-Valencia (BL = 11,414, I = 479) and WMC (BL = 15,902; I = 337), were cultured in a time-lapse system (EmbryoScope, Vitrolife, Sweden). Read More

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http://dx.doi.org/10.1007/s10815-018-1396-xDOI Listing
January 2019
1 Read

Morbid obesity-related changes in the expression of lipid receptors, transporters, and HSL in human sperm.

J Assist Reprod Genet 2019 Jan 18. Epub 2019 Jan 18.

Servicio de Bioquímica-Investigación, Madrid, Spain.

Objective: To study the location and expression of receptors (SR-BI/CLA-1, SR-BII, and LDLr) and transporter (ABCA1) involved in uptake and efflux of cholesterol in human spermatozoa and assess whether obesity alters its location/expression and whether this could be related to infertility.

Design: Observational study.

Setting: None PATIENT(S): Ten controls and 20 obese patients. Read More

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http://link.springer.com/10.1007/s10815-019-01406-z
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http://dx.doi.org/10.1007/s10815-019-01406-zDOI Listing
January 2019
4 Reads

Women's morbid conditions are associated with decreased odds of live birth in the first IVF/ICSI treatment: a retrospective single-center study.

J Assist Reprod Genet 2019 Jan 17. Epub 2019 Jan 17.

Department of Pediatrics, Obstetrics and Gynecology, Faculty of Medicine, University of Valencia, Valencia, Spain.

Purpose: The present study aims to ascertain whether there is a causal relationship between women's disease conditions present at the starting time of the first intended oocyte retrieval cycle and IVF/ICSI outcomes, primarily odds of live birth in the first IVF/ICSI treatment.

Methods: This is a retrospective study of infertile healthy and diseased women that had a live birth and/or exhibited a complete first oocyte retrieval cycle. Generalized Estimating Equations (GEE) models were applied to adjust standard errors for the potential correlation among women exhibiting the same infertility etiology. Read More

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http://dx.doi.org/10.1007/s10815-019-01401-4DOI Listing
January 2019
1 Read

Pregnancy rates after pre-implantation genetic screening for aneuploidy are only superior when trophectoderm biopsy is performed on hatching embryos.

J Assist Reprod Genet 2019 Jan 15. Epub 2019 Jan 15.

Obstetrics and Gynecology, Staten Island University Hospital, 475 Seaview Avenue, Staten Island, NY, 10305, USA.

Purpose: In vitro fertilization with trophectoderm embryo biopsy and pre-implantation genetic screening with comprehensive chromosomal screening (PGS-CCS) for aneuploidy is becoming increasingly more popular. Embryos are cryopreserved and implanted in a subsequent frozen thawed embryo transfer cycle (FET). No studies have investigated differences in pregnancy outcomes by timing of trophectoderm biopsy relative to stages of blastocyst development. Read More

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http://dx.doi.org/10.1007/s10815-019-01400-5DOI Listing
January 2019

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes.

J Assist Reprod Genet 2019 Jan 12. Epub 2019 Jan 12.

Faculty of Medicine, Department of Histology and Embryology, Masaryk University, Kamenice 3, 625 00, Brno, Czech Republic.

Propose: The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI).

Methods: The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. Read More

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http://link.springer.com/10.1007/s10815-018-1393-0
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http://dx.doi.org/10.1007/s10815-018-1393-0DOI Listing
January 2019
7 Reads

Cumulus-corona gene expression analysis combined with morphological embryo scoring in single embryo transfer cycles increases live birth after fresh transfer and decreases time to pregnancy.

J Assist Reprod Genet 2019 Jan 9. Epub 2019 Jan 9.

Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium.

Purpose: Clinical pregnancy rate after IVF with eSET stagnates between 30 and 40%. In order to increase pregnancy and live birth rates, multiple embryo transfer is still common practice. Providing additional non-invasive tools to choose the competent embryo for transfer could avoid multiple pregnancy and improve time to pregnancy. Read More

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http://dx.doi.org/10.1007/s10815-018-01398-2DOI Listing
January 2019

In assisted reproduction by IVF or ICSI, the rate at which embryos develop to the blastocyst stage is influenced by the fertilization method used: a split IVF/ICSI study.

J Assist Reprod Genet 2019 Jan 9. Epub 2019 Jan 9.

Centre for Reproductive and Genetic Health, London, UK.

Purpose: To compare in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI) in regard to post-fertilization development and outcome with the purpose of ascertaining the most effective fertilization method for assisted reproduction.

Methods: A retrospective cohort study of 136 split IVF/ICSI cycles (where sibling oocytes are fertilized by two different methods using the same sperm sample).

Results: IVF-derived embryos developed to the blastocyst stage at a significantly faster rate than ICSI-derived embryos. Read More

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http://link.springer.com/10.1007/s10815-018-1358-3
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http://dx.doi.org/10.1007/s10815-018-1358-3DOI Listing
January 2019
10 Reads

Single best euploid versus single best unknown-ploidy blastocyst frozen embryo transfers: a randomized controlled trial.

J Assist Reprod Genet 2019 Jan 7. Epub 2019 Jan 7.

Antalya IVF, Halide Edip Cd. No:7, Kanal Mh, 07080, Antalya, Turkey.

Purpose: This paper aims to investigate the efficacy of IVF with preimplantation genetic testing for aneuploidy (PGT-A), using only best-scoring blastocysts from young (≤ 35 years) infertile patients undergoing single blastocyst frozen embryo transfers (FET).

Method: In this randomized controlled trial (RCT) registered 29 March 2017, 302 infertile patient-couples eligible to participate underwent autologous ICSI blastocyst freeze-all cycles. Two-hundred and twenty patient-couples satisfied the inclusion criteria (i. Read More

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http://dx.doi.org/10.1007/s10815-018-01399-1DOI Listing
January 2019

Off the street phasing (OTSP): no hassle haplotype phasing for molecular PGD applications.

J Assist Reprod Genet 2019 Jan 8. Epub 2019 Jan 8.

Medical Genetics Institute, Shaare Zedek Medical Center (SZMC), Bayit Str. 12, P.O.Box 3235, 91031, Jerusalem, Israel.

Purpose: Pre-implantation genetic diagnosis (PGD) for molecular disorders requires the construction of parental haplotypes. Classically, haplotype resolution ("phasing") is obtained by genotyping multiple polymorphic markers in both parents and at least one additional relative. However, this process is time-consuming, and immediate family members are not always available. Read More

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http://link.springer.com/10.1007/s10815-018-1392-1
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http://dx.doi.org/10.1007/s10815-018-1392-1DOI Listing
January 2019
5 Reads

Hydatidiform molar pregnancy following assisted reproduction.

J Assist Reprod Genet 2019 Jan 5. Epub 2019 Jan 5.

Department of Reproductive Medicine, Saint Mary's Hospital, Manchester Academic Health Sciences Centre, Manchester University NHS Foundation Trust, Oxford Road, Manchester, M13 9WL, UK.

Introduction: The use of assisted reproduction techniques (ART) is increasing; however, reports of molar pregnancy following ART remain scarce. Currently, the Human Fertility and Embryology Authority (HFEA) collates data on the molar pregnancies that have resulted through the use of ART. Recently, they have indicated that they will no longer collect these data. Read More

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http://link.springer.com/10.1007/s10815-018-1389-9
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http://dx.doi.org/10.1007/s10815-018-1389-9DOI Listing
January 2019
4 Reads

Testosterone therapy for women with poor ovarian response undergoing IVF: a meta-analysis of randomized controlled trials.

J Assist Reprod Genet 2019 Jan 5. Epub 2019 Jan 5.

Instituto Valenciano de Infertilidad, IVI-RMA Global, Avenida del Talgo 68-70, 28023, Madrid, Spain.

Purpose: The aim of the present systematic review and meta-analysis was to summarize evidence on the effectiveness of testosterone supplementation for poor ovarian responders (POR) on IVF outcomes. The primary outcome was live birth rate (LBR); secondary outcomes were clinical pregnancy rate (CPR), miscarriage rate (MR), total and MII oocytes, and total embryos.

Methods: This meta-analysis of randomized controlled trials (RCTs) evaluates the effects of testosterone administration before/during COS compared with a control group in patients defined as POR. Read More

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http://link.springer.com/10.1007/s10815-018-1383-2
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http://dx.doi.org/10.1007/s10815-018-1383-2DOI Listing
January 2019
1 Read

Monkeys, mice and menses: the bloody anomaly of the spiny mouse.

J Assist Reprod Genet 2019 Jan 5. Epub 2019 Jan 5.

Centre for Reproductive Health, Hudson Institute of Medical Research, 27-31 Wright St, Clayton, 3168, Australia.

The common spiny mouse (Acomys cahirinus) is the only known rodent to demonstrate a myriad of physiological processes unseen in their murid relatives. The most recently discovered of these uncharacteristic traits: spontaneous decidual transformation of the uterus in virgin females, preceding menstruation. Menstruation occurring without experimental intervention in rodents has not been documented elsewhere to date, and natural menstruation is indeed rare in the animal kingdom outside of higher order primates. Read More

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http://dx.doi.org/10.1007/s10815-018-1390-3DOI Listing
January 2019

Successful in vitro maturation of oocytes in a woman with gonadotropin-resistant ovary syndrome associated with a novel combination of FSH receptor gene variants: a case report.

J Assist Reprod Genet 2019 Jan 5. Epub 2019 Jan 5.

Centre d'aide médicale à la procréation FERTILYS, Laval, Québec, Canada.

Infertility due to Gonadotropin-Resistant Ovary Syndrome (GROS) is a rare type of hypergonadotropic hypogonadism. Here, we report an original case of GROS, associated with compound heterozygous follicle-stimulating hormone receptor (FSHR) variants, in a woman who achieved a live birth by in vitro maturation (IVM) of her oocytes. This 31-year-old woman consulted our assisted reproduction center for a second opinion after having been advised, because of pervasive high serum follicle-stimulating hormone (FSH) levels, to pursue in vitro fertilization (IVF) with donor oocytes. Read More

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http://dx.doi.org/10.1007/s10815-018-1394-zDOI Listing
January 2019

Medical or surgical treatment before embryo transfer improves outcomes in women with abnormal endometrial BCL6 expression.

J Assist Reprod Genet 2019 Jan 4. Epub 2019 Jan 4.

Department of Obstetrics and Gynecology, Greenville Health System, 890 W. Faris Rd, Ste 470, Greenville, SC, 29605, USA.

Purpose: To evaluate the effect of medical or surgical treatment prior to embryo transfer in women with elevated endometrial BCL6 expression and suspected endometriosis in a prospective, cohort study design at a university-associated infertility clinic.

Methods: All subjects had at least 1 year of unexplained infertility (UI) and each prospectively underwent endometrial biopsy and immunostaining for the oncogene BCL6, prior to embryo transfer during an assisted reproductive technology (ART) cycle. To be included, subjects had to have an abnormal BCL6 result, defined by elevated HSCORE ≥ 1. Read More

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http://dx.doi.org/10.1007/s10815-018-1388-xDOI Listing
January 2019
1 Read

Platelet-rich plasma or blood-derived products to improve endometrial receptivity?

J Assist Reprod Genet 2019 Jan 4. Epub 2019 Jan 4.

nilo.frantz Medicina Reprodutiva, Porto Alegre, RS, Brazil.

The use of platelet-rich plasma (PRP) to improve endometrial receptivity is gaining increasing attention in assisted reproduction technologies. The authors report that autologous PRP intrauterine administration improves pregnancy and birth rates, particularly in cases of patients presenting poor endometrial growth. Different groups of scientists proposed a similar approach years ago using whole blood-derived products also to improve endometrial receptivity. Read More

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http://link.springer.com/10.1007/s10815-018-1386-z
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http://dx.doi.org/10.1007/s10815-018-1386-zDOI Listing
January 2019
1 Read

In vitro fertilization alters phospholipid profiles in mouse placenta.

J Assist Reprod Genet 2019 Jan 4. Epub 2019 Jan 4.

Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, China.

Purpose: Studies on humans and rodents have clearly shown that in vitro fertilization (IVF) is associated with abnormal placenta formation and function. Currently, dysregulated placental lipid metabolism is one of the emerging pathogenetic pathways implicated in adverse pregnancy outcomes. The purpose of this study was to identify the effects of IVF on lipid metabolism in the mouse placenta. Read More

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http://link.springer.com/10.1007/s10815-018-1387-y
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http://dx.doi.org/10.1007/s10815-018-1387-yDOI Listing
January 2019
4 Reads

Health care provider perceptions of fertility preservation barriers and challenges with transgender patients and families: qualitative responses to an international survey.

J Assist Reprod Genet 2019 Jan 3. Epub 2019 Jan 3.

Departments of OB-GYN and Population Health, New York University School of Medicine, New York, NY, 10016, USA.

Purpose: To examine provider perceptions of practice behaviors and barriers related to fertility counseling, fertility preservation, and family building among transgender patients.

Methods: Participants were medical and mental health professionals who treat adult and youth transgender patients. Recruitment occurred online and in person, via professional listservs for transgender professionals, conferences, and gender clinics. Read More

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http://link.springer.com/10.1007/s10815-018-1395-y
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http://dx.doi.org/10.1007/s10815-018-1395-yDOI Listing
January 2019
4 Reads

Male infertility: establishing sperm aneuploidy thresholds in the laboratory.

J Assist Reprod Genet 2019 Jan 2. Epub 2019 Jan 2.

Reproductive Genetics Unit, Sistemas Genómicos S.L, Ronda G. Marconi 6, Parque Tecnológico, 46980, Paterna, Valencia, Spain.

Purpose: Fluorescence in situ hybridization (FISH) in spermatozoa provides an estimate of the frequency of chromosomal abnormalities, but there is not a clinical consensus on how to statistically analyze sperm FISH results. We therefore propose a statistical approach to establish sperm aneuploidy thresholds in a fertile population.

Methods: We have determined the distribution and variation of the frequency of nullisomy, disomy, and diploidy for a set of 13 chromosomes (1, 2, 9, 13, 15, 16, 17, 18, 19, 21, 22, X, and Y) in sperm nuclei from 14 fertile men by means of automatized FISH. Read More

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http://dx.doi.org/10.1007/s10815-018-1385-0DOI Listing
January 2019

Whole-exome sequencing identified a novel mutation of AURKC in a Chinese family with macrozoospermia.

J Assist Reprod Genet 2018 Dec 29. Epub 2018 Dec 29.

Center for Reproductive Medicine, Anhui Provincial Hospital Affiliated to University of Science and Technology of China, Hefei City, Anhui Province, China.

Purpose: Macrozoospermia is a rare sperm morphologic abnormality associated with male infertility and is characterized by a high percentage of spermatozoa with large irregular heads. The aim of this study was to identify the genetic cause of an infertile male with macrozoospermia from a consanguineous family.

Methods: Whole-exome sequencing (WES) was performed using peripheral blood genomic DNA from the patient and his parents. Read More

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http://dx.doi.org/10.1007/s10815-018-1374-3DOI Listing
December 2018
1 Read

Editorial prerogatives taken to new heights in matters ARTs.

J Assist Reprod Genet 2019 Jan;36(1):1-2

Center for Human Reproduction, New York, NY, USA.

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http://dx.doi.org/10.1007/s10815-018-1391-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338587PMC
January 2019

Overcoming bioethical, legal, and hereditary barriers to mitochondrial replacement therapy in the USA.

J Assist Reprod Genet 2018 Dec 15. Epub 2018 Dec 15.

Exergen Corporation, Watertown, MA, USA.

The purpose of the paper is to explore novel means to overcome the controversial ban in the USA against mitochondrial replacement therapy, a form of IVF, with the added step of replacing a woman's diseased mutated mitochondria with a donor's healthy mitochondria to prevent debilitating and often fatal mitochondrial diseases. Long proven effective in non-human species, MRT recently performed in Mexico resulted in the birth of a healthy baby boy. We explore the ethics of the ban, the concerns over hereditability of mitochondrial disease and its mathematical basis, the overlooked role of Mitochondrial Eve, the financial burden of mitochondrial diseases for taxpayers, and a woman's reproductive rights. Read More

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http://dx.doi.org/10.1007/s10815-018-1370-7DOI Listing
December 2018

A subspecialty of the assisted reproductive technologies: cryogenic inventory maintenance.

Authors:
Kathryn J Go

J Assist Reprod Genet 2019 Jan 15;36(1):15-17. Epub 2018 Dec 15.

Boston IVF - the Maine Center, South Portland, ME, USA.

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http://dx.doi.org/10.1007/s10815-018-1378-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338608PMC
January 2019

Lipophilic statins inhibit growth and reduce invasiveness of human endometrial stromal cells.

J Assist Reprod Genet 2018 Dec 15. Epub 2018 Dec 15.

Division of Reproductive Endocrinology and Infertility, Department of Reproductive Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0633, USA.

Purpose: To compare effects of lipid-soluble statins (simvastatin, lovastatin, atorvastatin) and water-soluble statin (pravastatin) on growth and invasiveness of human endometrial stromal (HES) cells.

Methods: Endometrial biopsies were collected during the proliferative phase from five volunteers. HES cells were isolated and cultured in the absence or in the presence of simvastatin, lovastatin, atorvastatin, and pravastatin. Read More

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http://link.springer.com/10.1007/s10815-018-1352-9
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http://dx.doi.org/10.1007/s10815-018-1352-9DOI Listing
December 2018
5 Reads

Infertility patients with chromosome inversions are not susceptible to an inter-chromosomal effect.

J Assist Reprod Genet 2018 Dec 15. Epub 2018 Dec 15.

Colorado Center for Reproductive Medicine, 10290 RidgeGate Circle, Lone Tree, CO, 80124, USA.

Purpose: The aim of this study was to evaluate the incidence of an inter-chromosomal effect (ICE) in blastocyst-stage embryos from carriers of balanced chromosome inversions.

Methods: Infertility patients (n = 52) with balanced inversions (n = 66 cycles), and maternal age-matched controls that concurrently cycled (n = 66), consented to an IVF cycle with preimplantation genetic testing for aneuploidy (PGT-A). Blastocyst-stage embryos underwent trophectoderm biopsy for PGT-A with only euploid blastocysts transferred in a subsequent frozen embryo transfer. Read More

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http://dx.doi.org/10.1007/s10815-018-1376-1DOI Listing
December 2018

Lower follicular n-3 polyunsaturated fatty acid levels are associated with a better response to ovarian stimulation.

J Assist Reprod Genet 2018 Dec 13. Epub 2018 Dec 13.

Free Radicals and Oxidative Stress (FROS) Research Group of the Department of Physiology, Medicine and Nursing School, University of the Basque Country UPV/EHU, 48940, Leioa, Spain.

Objectives: To analyze in detail the fatty acid (FA) composition of follicular fluid (FF) from two-sized follicles at oocyte retrieval and to determine associations of the FAs from large follicles with the woman's age and the response to ovarian stimulation.

Design: Observational study.

Setting: University and fertility clinic. Read More

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http://link.springer.com/10.1007/s10815-018-1384-1
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http://dx.doi.org/10.1007/s10815-018-1384-1DOI Listing
December 2018
10 Reads

Effects of the microfluidic chip technique in sperm selection for intracytoplasmic sperm injection for unexplained infertility: a prospective, randomized controlled trial.

J Assist Reprod Genet 2018 Dec 12. Epub 2018 Dec 12.

Division of Reproductive Endocrinology and IVF Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Baskent University, Adana, Turkey.

Purpose: The new-generation spermatozoon selection method, microfluidic technique called Fertile Chip gives the chance to select spermatozoa with lower DNA fragmentation indexes. We aimed to determine the effect of microfluidic techniques for spermatozoon selection in ICSI treatment in patients with unexplained infertility.

Methods: This prospective randomized controlled study was conducted at a university hospital. Read More

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http://dx.doi.org/10.1007/s10815-018-1375-2DOI Listing
December 2018
2 Reads

Correlation of human telomerase reverse transcriptase single nucleotide polymorphisms with in vitro fertilisation outcomes.

J Assist Reprod Genet 2018 Dec 10. Epub 2018 Dec 10.

Reproductive Medicine Center, Department of Obstetrics and Gynaecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No.107, Yanjiang Xi Road, Guangzhou, 510120, Guangdong, People's Republic of China.

Purpose: To explore whether it is possible to predict the number and quality of the embryo using a few particular hTERT SNPs.

Methods: We included 997 Han Chinese women who were genetically unrelated and underwent assisted reproduction using IVF from September 2014 to December 2015. DNA was genotyped by using TaqMan real-time quantitative PCR. Read More

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http://link.springer.com/10.1007/s10815-018-1379-y
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http://dx.doi.org/10.1007/s10815-018-1379-yDOI Listing
December 2018
10 Reads

Transdermal versus oral estrogen: clinical outcomes in patients undergoing frozen-thawed single blastocyst transfer cycles without GnRHa suppression, a prospective randomized clinical trial.

J Assist Reprod Genet 2018 Dec 5. Epub 2018 Dec 5.

Assisted Reproductive Technologies and Reproductive Genetics Centre, Istanbul Memorial Hospital, Piyale Pasa Bulvari, 34385, Okmeydani, Istanbul, Turkey.

Purpose: To conduct a non-inferiority study to compare the clinical outcomes of transdermal estrogen patch and oral estrogen in patients undergoing frozen-thawed single blastocyst transfer non-donor cycles without GnRHagonist (GnRHa) suppression.

Methods: A total of 317 women with irregular menses or anovulatory cycle undergoing frozen-thawed embryo transfer (FET) non-donor cycles without GnRHa suppression were involved in a prospective randomized clinical trial between May 2017 and October 2017. The trial was conducted in an ART and Reproductive Genetics Centre within a private hospital. Read More

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http://link.springer.com/10.1007/s10815-018-1380-5
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http://dx.doi.org/10.1007/s10815-018-1380-5DOI Listing
December 2018
9 Reads

Bringing perspective to the next wave of human ARTs.

J Assist Reprod Genet 2018 Dec;35(12):2107-2108

Center for Human Reproduction, New York, NY, USA.

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http://dx.doi.org/10.1007/s10815-018-1382-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289925PMC
December 2018

Association between parental anthropometric measures and the outcomes of intracytoplasmic sperm injection cycles.

J Assist Reprod Genet 2018 Dec 5. Epub 2018 Dec 5.

Fertility Medical Group, Sao Paulo, 01401-002, Brazil.

Purpose: To study the association between body mass index (BMI), waist circumference (WC), waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR), and response to controlled ovarian stimulation, semen quality, and intracytoplasmic sperm injection (ICSI) outcomes.

Methods: This prospective observational study included data from 402 couples undergoing ICSI between January/2010-September/2016. Participants had their weight, height, waist, and hip measured before ICSI treatment. Read More

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http://dx.doi.org/10.1007/s10815-018-1381-4DOI Listing
December 2018
3 Reads

Rare genetic variants potentially involved in ovarian hyperstimulation syndrome.

J Assist Reprod Genet 2018 Nov 27. Epub 2018 Nov 27.

Center for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium.

Purpose: We aim to investigate whether there is a genetic predisposition in women who developed ovarian hyperstimulation syndrome (OHSS) after GnRH antagonist protocol with GnRH agonist trigger and freeze-all approach.

Methods: Four patients with OHSS after GnRH agonist trigger and freeze-all approach were gathered from the worldwide patient population. These patients were analyzed through Whole Exome Sequencing. Read More

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http://dx.doi.org/10.1007/s10815-018-1372-5DOI Listing
November 2018

Fertility preservation in women with malignancies: the accuracy of antral follicle count collected randomly during the menstrual cycle in predicting the number of oocytes retrieved.

J Assist Reprod Genet 2018 Nov 26. Epub 2018 Nov 26.

Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Purpose: To evaluate the capacity of random antral follicle count (AFC), i.e., AFC recorded at any time during the menstrual cycle, to predict the number of retrieved mature oocytes in women with malignancies undergoing random start ovarian hyperstimulation METHODS: A consecutive series of 72 women with malignancies who underwent ovarian hyperstimulation aimed at egg freezing between July 2014 and December 2016 was retrospectively reviewed. Read More

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http://link.springer.com/10.1007/s10815-018-1377-0
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http://dx.doi.org/10.1007/s10815-018-1377-0DOI Listing
November 2018
5 Reads

Effect of intra-ovarian injection of mesenchymal stem cells in aged mares.

J Assist Reprod Genet 2018 Nov 23. Epub 2018 Nov 23.

Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, 77843-4466, USA.

Purpose: This study aims to determine if intra-ovarian injection of bone marrow-derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females.

Methods: Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Read More

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http://dx.doi.org/10.1007/s10815-018-1371-6DOI Listing
November 2018
9 Reads
1.772 Impact Factor

Chromosomal abnormalities in couples with recurrent spontaneous miscarriage: a 21-year retrospective study, a report of a novel insertion, and a literature review.

J Assist Reprod Genet 2018 Nov 23. Epub 2018 Nov 23.

Laboratory of Genomics and Human Genetics, Département de la Recherche Scientifique, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360, Casablanca, Morocco.

Purpose: The aim of this study is to evaluate the frequency and nature of chromosomal abnormalities in Moroccan couples with recurrent spontaneous miscarriage (RSM). In addition, the data were compared with those reported elsewhere in order to give a global estimation of chromosomal abnormalities frequencies.

Methods: The study was performed for all couples with RSM who were referred to the cytogenetic department, Pasteur Institute of Morocco, from different hospitals in Morocco between 1996 and 2016. Read More

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http://dx.doi.org/10.1007/s10815-018-1373-4DOI Listing
November 2018
10 Reads

Can peri-ovulatory putrescine supplementation improve egg quality in older infertile women?

J Assist Reprod Genet 2018 Nov 22. Epub 2018 Nov 22.

Ottawa Hospital Research Institute, The Ottawa Hospital-General Campus, 501 Smyth Road, Box 511, Ottawa, K1H 8L6, Canada.

The aging-related decline in fertility is an increasingly pressing medical and economic issue in modern society where women are delaying family building. Increasingly sophisticated, costly, and often increasingly invasive, assisted reproductive clinical protocols and laboratory technologies (ART) have helped many older women achieve their reproductive goals. Current ART procedures have not been able to address the fundamental problem of oocyte aging, the increased rate of egg aneuploidy, and the decline of developmental potential of the eggs. Read More

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http://dx.doi.org/10.1007/s10815-018-1327-xDOI Listing
November 2018
29 Reads

Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos.

J Assist Reprod Genet 2018 Nov 16. Epub 2018 Nov 16.

Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg C, Denmark.

Purpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently "primed" for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Read More

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http://dx.doi.org/10.1007/s10815-018-1365-4DOI Listing
November 2018
1 Read

Chromosome constitution of equal-sized three-cell embryos using next-generation sequencing technology.

J Assist Reprod Genet 2018 Nov 16. Epub 2018 Nov 16.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, 100853, China.

Purpose: To study the chromosome constitution of equal-sized three-cell embryo.

Methods: We determined the chromosome constitution of 105 blastomeres from 35 embryos using multiple annealing and looping-based amplification cycles (MALBAC) together with NGS sequencing technology. Chromosomal copy number variation (CNV) analysis was successfully performed in 27 embryos. Read More

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http://dx.doi.org/10.1007/s10815-018-1362-7DOI Listing
November 2018
2 Reads

Treatment of female rhesus macaques with a somatostatin receptor antagonist that increases oocyte fertilization rates without affecting post-fertilization development outcomes.

J Assist Reprod Genet 2018 Nov 15. Epub 2018 Nov 15.

Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA.

Purpose: To determine the effects of PGL1001, a somatostatin receptor isoform-2 (SSTR-2) antagonist, on ovarian follicle development, oocyte fertilization, and subsequent embryo developmental potential in the rhesus macaque.

Methods: Cycling female rhesus macaques (N = 8) received vehicle through one menstrual (control) cycle, followed by daily injections of PGL1001, a SSTR-2 antagonist, for three menstrual (treatment) cycles. Main endpoints include overall animal health and ovarian hormones (e. Read More

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http://link.springer.com/10.1007/s10815-018-1369-0
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http://dx.doi.org/10.1007/s10815-018-1369-0DOI Listing
November 2018
7 Reads

Single blastomeres as a source of mouse embryonic stem cells: effect of genetic background, medium supplements, and signaling modulators on derivation efficiency.

J Assist Reprod Genet 2019 Jan 14;36(1):99-111. Epub 2018 Nov 14.

Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Spain.

Purpose: To assess the role of the genetic background, the culture medium supplements, and the presence of modulators of signaling pathways on mouse embryonic stem cell derivation from single blastomeres from 8-cell embryos.

Methods: Mice from permissive and non-permissive genetic backgrounds, different culture media supplements, knockout serum replacement (KSR) and N2B27, and the presence or absence of 2i treatment were used to derive mouse embryonic stem cells (mESC) from single blastomeres isolated from 8-cell embryos and from control embryos at the blastocyst stage. After the sixth passage, the putative mESC were analyzed by immunofluorescence to assess their pluripotency and, after in vitro differentiation induction, their ability to differentiate into derivatives of the three primary germ layers. Read More

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http://link.springer.com/10.1007/s10815-018-1360-9
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http://dx.doi.org/10.1007/s10815-018-1360-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338609PMC
January 2019
11 Reads

Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations.

J Assist Reprod Genet 2018 Nov 12. Epub 2018 Nov 12.

IVF Lab & Wolfe PGD-Stem Cell Lab, Racine IVF Unit, Lis Maternity Hospital, Tel-Aviv Sourasky Medical Center, Tel Aviv, Israel, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 6 Weizman Street, 6423906, Tel Aviv, Israel.

Purpose: The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy.

Methods: The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. Read More

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http://dx.doi.org/10.1007/s10815-018-1361-8DOI Listing
November 2018
2 Reads