3,909 results match your criteria Journal of Assisted Reproduction and Genetics [Journal]


Understanding mechanisms of oocyte development by follicular fluid lipidomics.

J Assist Reprod Genet 2019 Apr 23. Epub 2019 Apr 23.

Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Rua Embau, 231, CEP: 04039-060, Sao Paulo, SP, Brazil.

Purpose: The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development.

Methods: To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Read More

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http://dx.doi.org/10.1007/s10815-019-01428-7DOI Listing

Gene expression analysis of follicular cells revealed inflammation as a potential IVF failure cause.

J Assist Reprod Genet 2019 Apr 18. Epub 2019 Apr 18.

Centre de recherche en reproduction, développement et santé intergénérationnelle (CRDSI), Université Laval, 2440 Boulevard Hochelaga, Québec, QC, G1V 0A6, Canada.

Purpose: Hormonal stimulation prior to IVF influences the ovarian environment and therefore impacts oocytes and subsequent embryo quality. Not every patient has the same response to the same treatment and many fail for unknown reasons. Knowing why a cycle has failed and how the follicles were affected would allow clinicians to adapt the treatment accordingly and improve success rate. Read More

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http://dx.doi.org/10.1007/s10815-019-01447-4DOI Listing

Is a frozen embryo transfer in a programmed cycle really the best option?

J Assist Reprod Genet 2019 Apr 13. Epub 2019 Apr 13.

Division of Reproductive Sciences and Women's Health Research, Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, 720 Rutland Ave, Baltimore, MD, 21205, USA.

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http://link.springer.com/10.1007/s10815-019-01449-2
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http://dx.doi.org/10.1007/s10815-019-01449-2DOI Listing
April 2019
2 Reads

Apoptosis of mural granulosa cells is increased in women with diminished ovarian reserve.

J Assist Reprod Genet 2019 Apr 13. Epub 2019 Apr 13.

Reproductive Medical Center, The Sixth Affiliated Hospital of Sun Yat-sen University, 17th Shou-gou-ling Rd, Guangzhou, 510655, China.

Purpose: To evaluate the relationship between apoptosis of granulosa cells in women with normal ovarian reserve versus diminished ovarian reserve, and relate that to follicular fluid hormones, and to clinical outcomes.

Methods: A prospective cohort study was initiated between October 2015 and June 2016 involving a total of 164 women undergoing IVF/ICSI cycles at a single IVF center. Mural and cumulus granulosa cells, and follicularfluid were collected during oocyte retrieval. Read More

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http://link.springer.com/10.1007/s10815-019-01446-5
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http://dx.doi.org/10.1007/s10815-019-01446-5DOI Listing
April 2019
2 Reads

Transcriptomic analysis of the interaction of choriocarcinoma spheroids with receptive vs. non-receptive endometrial epithelium cell lines: an in vitro model for human implantation.

J Assist Reprod Genet 2019 Apr 10. Epub 2019 Apr 10.

Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.

Purpose: Several in vitro systems have been reported to model human implantation; however, the molecular dynamics of the trophoblast vs. the epithelial substrate during attachment have not been described. We have established an in vitro model which allowed us to dissect the transcriptional responses of the trophoblast and the receptive vs. Read More

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http://dx.doi.org/10.1007/s10815-019-01442-9DOI Listing
April 2019
3 Reads

Body mass index restrictions in fertility treatment: a national survey of OB/GYN subspecialists.

J Assist Reprod Genet 2019 Apr 8. Epub 2019 Apr 8.

Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, 48108, USA.

Purpose: To explore the attitudes of reproductive endocrinology and infertility (REI) and maternal-fetal medicine (MFM) subspecialists regarding the necessity and appropriateness of body mass index (BMI) cutoffs for women seeking fertility treatment.

Methods: Members of the Society for Reproductive Endocrinology and Infertility (SREI) and the Society for Maternal Fetal Medicine (SMFM) were invited to participate in a survey querying their knowledge of existing institutional or clinic BMI policies and personal opinions regarding upper and lower BMI cutoffs for a range of fertility treatments, including oral ovulation agents, gonadotropins, and in vitro fertilization.

Results: Respondents included 398 MFMs and 201 REIs. Read More

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http://dx.doi.org/10.1007/s10815-019-01448-3DOI Listing
April 2019
2 Reads

Successful embryo cryopreservation immediately following a full-term delivery in a woman with newly diagnosed Ewing's sarcoma.

J Assist Reprod Genet 2019 Apr 5. Epub 2019 Apr 5.

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Drive, Ann Arbor, MI, 48109-5276, USA.

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http://dx.doi.org/10.1007/s10815-019-01445-6DOI Listing
April 2019
2 Reads

Which embryo selection method should be offered to the patients?

J Assist Reprod Genet 2019 Apr 5. Epub 2019 Apr 5.

Division of Reproductive Endocrinology And Infertility, Department of Obstetrics & Gynecology and Women's Health, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, New York, NY, USA.

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http://dx.doi.org/10.1007/s10815-019-01443-8DOI Listing
April 2019
1 Read

The role of FAS, FAS-L, BAX, and BCL-2 gene polymorphisms in determining susceptibility to unexplained recurrent pregnancy loss.

J Assist Reprod Genet 2019 Apr 2. Epub 2019 Apr 2.

Department of Genetics, Institute of Biosciences, Universidade Federal do Rio Grande do Sul (UFRGS), Bento Gonçalves Avenue 9500, Campus do Vale, Porto Alegre, RS, CEP 91501970, Brazil.

Purpose: Idiopathic recurrent pregnancy loss (RPL) is a multifactorial reproductive disorder where an impaired control of apoptosis is likely involved. Triggering the cell death mechanism occurs in a spatiotemporal manner and is strongly related to a healthy pregnancy. Single nucleotide polymorphisms (SNPs) at the regulatory regions of genes are known to influence the expression patterns of apoptosis-related molecules. Read More

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http://dx.doi.org/10.1007/s10815-019-01441-wDOI Listing
April 2019
1 Read

Observation of two separate bipolar spindles in the human zygote.

J Assist Reprod Genet 2019 Mar 30. Epub 2019 Mar 30.

Reproductive Medicine Center, Henan Provincial People's Hospital, Zhengzhou, Henan, China.

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http://dx.doi.org/10.1007/s10815-019-01440-xDOI Listing
March 2019
1.772 Impact Factor

Increased pregnancy complications following frozen-thawed embryo transfer during an artificial cycle.

J Assist Reprod Genet 2019 Mar 29. Epub 2019 Mar 29.

Institute of Reproduction and Stem Cell Engineering, School of Basic Medicine, Central South University, Changsha, China.

Purpose: This study aimed to clarify the risks of adverse pregnancy outcomes in patients and their offspring after frozen embryo transfer (FET) during an artificial cycle (AC).

Methods: We conducted a retrospective cohort study that included all FET cycles and subsequent deliveries in a single centre between August 2013 and March 2016. Pregnancy, obstetric and neonatal outcomes were compared among patients treated during an AC or a natural cycle with luteal phase support (NC-LPS). Read More

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http://dx.doi.org/10.1007/s10815-019-01420-1DOI Listing
March 2019
3 Reads

mTOR inhibitor sirolimus negatively impacts in vitro fertilization outcomes.

J Assist Reprod Genet 2019 Mar 25. Epub 2019 Mar 25.

Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, 499 Illinois St, San Francisco, CA, 94158, USA.

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http://link.springer.com/10.1007/s10815-019-01439-4
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http://dx.doi.org/10.1007/s10815-019-01439-4DOI Listing
March 2019
3 Reads

Current status of spent embryo media research for preimplantation genetic testing.

J Assist Reprod Genet 2019 Mar 21. Epub 2019 Mar 21.

Progenesis Inc., 4150 Regents Park Row, Suite 245, La Jolla, CA, 92037, USA.

In recent years, a growing body of literature has emerged investigating the clinical utility of spent embryo media (SEM) for preimplantation genetic testing for aneuploidy (PGT-A) (Hammond et al. in Fertil Steril. 107(1):220-8, 2017; Xu et al. Read More

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http://dx.doi.org/10.1007/s10815-019-01437-6DOI Listing

The clinical application of preimplantation genetic diagnosis for X-linked retinitis pigmentosa.

J Assist Reprod Genet 2019 Mar 19. Epub 2019 Mar 19.

Research Center for Molecular Diagnosis of Genetic Diseases, Fuzhou General Hospital, Clinical College of Fujian Medical University/Dongfang Hospital, Xiamen University Medical College, 156 Xi'erhuanbei Road, Fuzhou City, 350025, Fujian Province, People's Republic of China.

Objective: To investigate the usefulness of preimplantation genetic diagnosis (PGD) based on mutated allele revealed by sequencing with aneuploidy and linkage analyses (MARSALA) for a pedigree with X-linked retinitis pigmentosa (XLRP).

Methods: One pathogenic mutation (c.494G > A) of the retinitis pigmentosa GTPase regulator (RPGR) gene was identified in a pedigree affected by XLRP. Read More

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http://dx.doi.org/10.1007/s10815-019-01434-9DOI Listing
March 2019
1 Read

Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte.

J Assist Reprod Genet 2019 Mar 18. Epub 2019 Mar 18.

Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista, Rubião Junior, Botucatu, São Paulo, 18618-970, Brazil.

Purpose: We first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.

Methods: In vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Read More

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http://dx.doi.org/10.1007/s10815-019-01436-7DOI Listing

Association of genetic polymorphism of vascular endothelial growth factor in the etiology of recurrent pregnancy loss: a triad study.

J Assist Reprod Genet 2019 Mar 15. Epub 2019 Mar 15.

Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016, India.

Purpose: The study estimates the association of VEGF gene polymorphism (-1154 G/A, -2549 I/D, -2578 C/A, and +936 C/T) in recurrent pregnancy loss from South Indian population.

Methods: A total of 100 couples with the history of recurrent pregnancy loss and 100 couples with medically terminated pregnancies were considered. Fetal tissues with < 20 weeks of gestation including peripheral blood from case and control couples were collected. Read More

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http://link.springer.com/10.1007/s10815-019-01431-y
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http://dx.doi.org/10.1007/s10815-019-01431-yDOI Listing
March 2019
12 Reads

Transcription profile of the insulin-like growth factor signaling pathway during human ovarian follicular development.

J Assist Reprod Genet 2019 Mar 15. Epub 2019 Mar 15.

Laboratory of Reproductive Biology, The Juliane Marie Centre for Women, Children and Reproduction, Rigshospitalet, Copenhagen University Hospital, Copenhagen University, DK-2100, Copenhagen, Denmark.

Purpose: The IGF signaling cascade exerts important regulatory functions in human ovarian folliculogenesis. The scope of this study was to evaluate the transcription profile of insulin-like growth factor (IGF) genes during human ovarian follicle development and to analyze follicle fluid levels of key IGF proteins.

Methods: Gene expression profiling was performed with microarray gene analysis. Read More

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http://dx.doi.org/10.1007/s10815-019-01432-xDOI Listing
March 2019
1 Read

In vivo veritas, in vitro-not so sure when it comes to ARTs.

J Assist Reprod Genet 2019 03;36(3):361-362

Center for Human Reproduction, New York, NY, USA.

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http://dx.doi.org/10.1007/s10815-019-01435-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439108PMC
March 2019
1 Read

PEX10, SIRPA-SIRPG, and SOX5 gene polymorphisms are strongly associated with nonobstructive azoospermia susceptibility.

J Assist Reprod Genet 2019 Mar 13. Epub 2019 Mar 13.

Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430032, China.

Purpose: Male infertility is a multifactorial syndrome encompassing a wide variety of disorders. A previous Chinese genome-wide single-nucleotide polymorphism (SNP) association studies have identified four SNPs (rs12097821 in PRMT6 gene, rs2477686 in PEX10 gene, rs6080550 in SIRPA-SIRPG, and rs10842262 in SOX5 gene) as being significantly associated with risk factors for nonobstructive azoospermia (NOA). However, the results were not fully repeated in later studies, which calls for further investigations. Read More

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http://link.springer.com/10.1007/s10815-019-01417-w
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http://dx.doi.org/10.1007/s10815-019-01417-wDOI Listing
March 2019
7 Reads

Unlocking the mysteries of the cumulus-oocyte complex-a critical cellular partnership for developmental competence.

J Assist Reprod Genet 2019 03 12;36(3):411-412. Epub 2019 Mar 12.

Smithsonian Conservation Biology Institute, National Zoological Park, Washington, DC, USA.

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http://dx.doi.org/10.1007/s10815-019-01421-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439104PMC

Guidance for elective single-embryo transfer should be applied to frozen embryo transfer cycles.

J Assist Reprod Genet 2019 Mar 11. Epub 2019 Mar 11.

Nashville Fertility Center, 345 23rd Ave. N., Suite 401, Nashville, TN, 37203, USA.

Purpose: To provide clinicians with data showing the benefits of transferring a single blastocyst in frozen embryo transfer (FET) cycles so that they may counsel their patients accordingly.

Methods: This is a closed cohort study of 678 FET cycles occurring between January 2011 and December 2017 in a private IVF laboratory and associated physicians' practice. Patients included in the analysis were less than 38 years of age at oocyte collection, had at least two vitrified blastocysts, and were undergoing their first autologous FET cycle. Read More

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http://dx.doi.org/10.1007/s10815-019-01433-wDOI Listing
March 2019
1 Read

Double chromosomal translocation in an infertile man: one-step FISH meiotic segregation analysis and reproductive prognosis.

J Assist Reprod Genet 2019 Mar 8. Epub 2019 Mar 8.

Département de Génétique Médicale, Assistance Publique-Hôpitaux de Paris, Hôpital Armand Trousseau, Unité INSERM 933, 26 avenue du Dr Arnold Netter, 75012, Paris, France.

Background: The prevalence of chromosomal translocations is 1/500 in the general population. While in the vast majority of cases, carriers have a normal phenotype; they can present with difficulty conceiving due to the presence of a proportion of unbalanced gametes as a consequence of abnormal chromosomal segregation during meiosis. Since complex translocations involve three or more chromosomes, meiotic segregation leads to a greater number of possible combinations which effectively complicate both their study and therapeutic care. Read More

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http://dx.doi.org/10.1007/s10815-019-01430-zDOI Listing

Endometrial lymphocyte concentrations in adverse reproductive outcome populations.

J Assist Reprod Genet 2019 Mar 7. Epub 2019 Mar 7.

RCSI, Beaumont Hospital, Dublin, Ireland.

Purpose: The uterine immunophenotype is relatively poorly understood, with most studies reporting proportions/percentages. A novel technique to calculate local endometrial lymphocyte concentrations is described, and used to compare results between aetiological subgroups such as repeated implantation failure (RIF) and recurrent pregnancy loss (RPL) with male-factor controls.

Methods: 455 patients had an endometrial biopsy performed. Read More

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http://dx.doi.org/10.1007/s10815-019-01427-8DOI Listing

Understanding the role of killer cell immunoglobulin-like receptors in pregnancy complications.

J Assist Reprod Genet 2019 Mar 7. Epub 2019 Mar 7.

Inmunología, Centro de Investigaciones Biomédicas (CINBIO), Universidade de Vigo, Vigo, Spain.

Pregnancy is a unique immunological situation in which a fetus-bearing paternal histocompatibility antigens can survive in a maternal environment without apparent rejection. To face this challenge, cells of the uterine immune system show characteristic changes in absolute number and composition during pregnancy. Particularly relevant to this process are uterine natural killer (uNK) cells and their cell surface receptors, killer immunoglobulin-like receptors (KIRs). Read More

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http://dx.doi.org/10.1007/s10815-019-01426-9DOI Listing

Early detection of cryostorage tank failure using a weight-based monitoring system.

J Assist Reprod Genet 2019 Mar 5. Epub 2019 Mar 5.

Columbia University Fertility Center, 5 Columbus Circle, PH, New York, NY, 10019, USA.

Purpose: To study the relationship between liquid nitrogen loss and temperature in cryostorage dewars and develop an early-warning alarm for impending tank failure.

Methods: Cryostorage dewars were placed on custom-engineered scales, and weight and temperature data were continuously monitored in the setting of slow, medium, and fast rate-loss of LN to simulate three scenarios of tank failure.

Results: LN Tank weights and temperatures were continuously monitored and recorded, with a calculated alarm trigger set at 10% weight loss and temperature of - 185 °C. Read More

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http://dx.doi.org/10.1007/s10815-019-01402-3DOI Listing
March 2019
2 Reads
1.772 Impact Factor

Homozygous missense mutation Arg207Cys in the WEE2 gene causes female infertility and fertilization failure.

J Assist Reprod Genet 2019 Mar 2. Epub 2019 Mar 2.

State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: To investigate a novel mutation in the WEE2 gene in a female patient with primary infertility and fertilization failure.

Methods: Sanger sequencing was used to detect mutations in WEE2. The pathogenicity of the identified variant and its possible effects on the WEE2 protein were evaluated with in silico tools and molecular modeling. Read More

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http://dx.doi.org/10.1007/s10815-019-01418-9DOI Listing
March 2019
2 Reads

Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma.

J Assist Reprod Genet 2019 Feb 28. Epub 2019 Feb 28.

Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: Methylated cell-free fetal DNA (cffDNA) in maternal plasma can potentially be used as a biomarker for accurate noninvasive prenatal testing (NIPT) of fetal disorders. Recovery and purification of cffDNA are key steps for downstream applications. In this study, we aimed to developed and evaluated different aspects of an optimized method and compared its efficiency with common methods used for extraction of methylated cffDNA. Read More

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http://dx.doi.org/10.1007/s10815-019-01425-wDOI Listing
February 2019
1 Read
1.772 Impact Factor

Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing.

J Assist Reprod Genet 2019 Feb 25. Epub 2019 Feb 25.

Unit of Genetics, Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Purpose: To evaluate the effect of sperm concentration adjustment in human ejaculates on the sperm DNA quality and longevity.

Methods: Semen samples were obtained from 30 donors with a normal spermiogram. Following centrifugation, the sperm pellet was resuspended in PBS, and the sperm concentration adjusted to 200, 100, 50, 25, 12, and 6 × 10/mL. Read More

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http://dx.doi.org/10.1007/s10815-019-01423-yDOI Listing
February 2019
2 Reads

Prediction of live birth and cumulative live birth rates in freeze-all-IVF treatment of a general population.

J Assist Reprod Genet 2019 Feb 21. Epub 2019 Feb 21.

Antalya IVF, Halide Edip Cd. No:7, Kanal Mh., 07080, Antalya, Turkey.

Purpose: To investigate the cumulative live birth (cLB) rate of one complete freeze-all-IVF cycle in a general infertile population and to investigate patient and treatment variables that predict blastocyst development and live birth (LB).

Method: In a retrospective observational study, the data of all IVF cycles performed between 1 February 2015 and 31 January 2016 at a single IVF centre was investigated. In the study, patient-couples were followed up for 18 months following oocyte retrieval. Read More

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http://dx.doi.org/10.1007/s10815-019-01422-zDOI Listing
February 2019

Oncofertility: insights from IVF specialists-a worldwide web-based survey analysis.

J Assist Reprod Genet 2019 Feb 20. Epub 2019 Feb 20.

The Reproductive Medicine and IVF Unit, Kaplan Medical Center, 76100, Rehovot, Israel.

Purpose: This research sought to understand IVF-physicians' knowledge of, experience with, and attitudes toward fertility preservation for cancer patients.

Methods: A 35-question, self-report survey request was emailed to IVF providers who were registered on the IVF-Worldwide.com network (3826 clinics). Read More

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http://dx.doi.org/10.1007/s10815-019-01419-8DOI Listing
February 2019

Novel mutation in the ZP1 gene and clinical implications.

J Assist Reprod Genet 2019 Feb 18. Epub 2019 Feb 18.

IVF Center, Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, Guangdong, China.

Purpose: Empty follicle syndrome (EFS) is a complex reproductive disorder characterized by the repeated failure to aspirate oocytes from mature ovarian follicles during in vitro fertilization (IVF). In addition to some cases caused by iatrogenic problems and known genetic factors, there are still many unexplained aspects of EFS. Here, we aimed to assess the clinical and genetic characteristics of two EFS patients. Read More

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http://link.springer.com/10.1007/s10815-019-01404-1
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http://dx.doi.org/10.1007/s10815-019-01404-1DOI Listing
February 2019
6 Reads

The information highway between conceptus and soma: when conversations get confused.

J Assist Reprod Genet 2019 Feb;36(2):177-178

Center for Human Reproduction, New York, NY, USA.

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http://dx.doi.org/10.1007/s10815-019-01416-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420514PMC
February 2019

Expanded genetic carrier screening in clinical practice: a current survey of patient impressions and attitudes.

J Assist Reprod Genet 2019 Feb 13. Epub 2019 Feb 13.

Department of Obstetrics and Gynecology, Boonshoft School of Medicine, Wright State University, 128 Apple Street, Suite 3800 Weber CHE, Dayton, OH, 45409, USA.

Purpose: Expanded genetic carrier screening (ECS) is an important part of gynecological practice and preconception planning. We evaluated the awareness and attitudes among women regarding ECS and factors that may influence decision-making in a family planning context.

Methods: A 32-question survey in an academic university practice was given to 521 women who were either currently pregnant (n = 108), undergoing gynecologic care who were considering future fertility (n = 308), and considering or receiving fertility treatment (n = 105). Read More

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http://dx.doi.org/10.1007/s10815-019-01414-zDOI Listing
February 2019
2 Reads

Preimplantation genetic testing using Karyomapping for a paternally inherited reciprocal translocation: a case study.

J Assist Reprod Genet 2019 Feb 12. Epub 2019 Feb 12.

Monash IVF, Suite 1, 252 Clayton Rd, Clayton, Victoria, 3168, Australia.

Purpose: Preimplantation genetic testing (PGT) using Karyomapping is used to screen embryos for single gene disorders prior to implantation. While Karyomapping is not designed to screen for abnormalities in chromosome copy number, this testing is based upon a genome-wide analysis of single nucleotide polymorphisms (SNP) and, as such, some chromosome abnormalities are detected. The aim of this study was to validate whether Karyomapping could provide reliable and accurate PGT for a paternal 46,XY,t(10;19)(p15;p13. Read More

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http://link.springer.com/10.1007/s10815-019-01413-0
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http://dx.doi.org/10.1007/s10815-019-01413-0DOI Listing
February 2019
2 Reads

Maternal age, history of miscarriage, and embryonic/fetal size are associated with cytogenetic results of spontaneous early miscarriages.

J Assist Reprod Genet 2019 Feb 9. Epub 2019 Feb 9.

Center for Maternal-Fetal, Neonatal and Reproductive Medicine, National Center for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.

Purpose: To clarify the associations of the maternal age, history of miscarriage, and embryonic/fetal size at miscarriage with the frequencies and profiles of cytogenetic abnormalities detected in spontaneous early miscarriages.

Methods: Miscarriages before 12 weeks of gestation, whose karyotypes were evaluated by G-banding between May 1, 2005, and May 31, 2017, were included in this study. The relationships between their karyotypes and clinical findings were assessed using trend or chi-square/Fisher's exact tests and multivariate logistic analyses. Read More

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http://link.springer.com/10.1007/s10815-019-01415-y
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http://dx.doi.org/10.1007/s10815-019-01415-yDOI Listing
February 2019
5 Reads

Human β-defensin 1 in follicular fluid and semen: impact on fertility.

J Assist Reprod Genet 2019 Feb 2. Epub 2019 Feb 2.

Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy.

Purpose: β-defensins are antimicrobial peptides expressed at mucosal level of male and female genito-urinary tract, where they exert protective functions against infections, possibly preserving human health and fertility. In our study, we investigated the possible involvement of β-defensins in female and male infertility in Italian infertile couples (i) evaluating the presence of human β-defensin 1 (hBD-1) in follicular fluid (FF) and its correlation with in vitro fertilization (IVF) outcomes; (ii) investigating the relationship between hBD-1 levels in semen and IVF outcomes (comprising correlation with sperm parameters); and (iii) exploring the effect of hBD-1 peptide on spermatozoa motility in vitro.

Methods: A perspective observational analytic pilot study was conducted. Read More

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http://dx.doi.org/10.1007/s10815-019-01409-wDOI Listing
February 2019
3 Reads

An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploidy.

J Assist Reprod Genet 2019 Feb 1. Epub 2019 Feb 1.

DAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy.

Purpose: To investigate the association of cumulus cell (CC)-related expression of a selected cluster of key genes (PTGS2, CAMK1D, HAS2, STC1, and EFNB2) with embryo development to blastocyst.

Methods: Exploratory study at a private clinic. Eighteen advanced maternal age patients were enrolled (37. Read More

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http://dx.doi.org/10.1007/s10815-019-01410-3DOI Listing
February 2019

Artificial intelligence and machine learning for human reproduction and embryology presented at ASRM and ESHRE 2018.

J Assist Reprod Genet 2019 Jan 28. Epub 2019 Jan 28.

Department of Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Sixteen artificial intelligence (AI) and machine learning (ML) approaches were reported at the 2018 annual congresses of the American Society for Reproductive Biology (9) and European Society for Human Reproduction and Embryology (7). Nearly every aspect of patient care was investigated, including sperm morphology, sperm identification, identification of empty or oocyte containing follicles, predicting embryo cell stages, predicting blastocyst formation from oocytes, assessing human blastocyst quality, predicting live birth from blastocysts, improving embryo selection, and for developing optimal IVF stimulation protocols. This represents a substantial increase in reports over 2017, where just one abstract each was reported at ASRM (AI) and ESHRE (ML). Read More

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http://link.springer.com/10.1007/s10815-019-01408-x
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http://dx.doi.org/10.1007/s10815-019-01408-xDOI Listing
January 2019
6 Reads

Ethnical and sociocultural differences causing infertility are poorly understood-insights from the Arabian perspective.

J Assist Reprod Genet 2019 Jan 26. Epub 2019 Jan 26.

IVIRMA Middle-East Fertility Clinic, Marina Village, Villa B 22, POB 60202, Abu Dhabi, United Arab Emirates.

Infertility is acknowledged worldwide as a major health concern. Although global levels of primary and secondary infertility have hardly changed between 1990 and 2010, significant regional differences have been reported. The prevalence of infertility in women has been estimated to be one in every seven couples in the western world and one in every four couples in developing countries. Read More

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http://dx.doi.org/10.1007/s10815-019-01411-2DOI Listing
January 2019
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Obstetrical complications of thin endometrium in assisted reproductive technologies: a systematic review.

J Assist Reprod Genet 2019 Jan 25. Epub 2019 Jan 25.

Virginia Center for Reproductive Medicine, Reston, VA, USA.

Purpose: The aim of the systematic review is to describe the obstetrical complications associated with pregnancies in the context of a thin endometrial lining.

Methods: Systematic review of the literature.

Results: Patients who conceive in the setting of a thin endometrium have a significantly increased risk of early pregnancy loss, namely miscarriage and ectopic pregnancy. Read More

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http://dx.doi.org/10.1007/s10815-019-01407-yDOI Listing
January 2019
4 Reads

The interaction effect of angiogenesis and endothelial dysfunction-related gene variants increases the susceptibility of recurrent pregnancy loss.

J Assist Reprod Genet 2019 Jan 24. Epub 2019 Jan 24.

Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

Purpose: The role of genetic polymorphisms in the pathogenesis of recurrent pregnancy loss (RPL) has been studied intensively. Complex diseases, including miscarriage, are believed to have a polygenic basis, and gene-gene interactions can play a significant role in the etiology of the disease. This study was conducted to investigate the association of gene-gene interactions with angiogenesis, endothelial dysfunction-related gene polymorphisms, and RPL. Read More

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http://dx.doi.org/10.1007/s10815-019-01403-2DOI Listing
January 2019

Unbalanced X;9 translocation in an infertile male with de novo duplication Xp22.31p22.33.

J Assist Reprod Genet 2019 Jan 24. Epub 2019 Jan 24.

Laboratory of Genetics, Center of Experimental Medicine and Translational Research, Biomedical Research Foundation of the Academy of Athens, (BRFAA), Athens, Greece.

Purpose: Male carriers of an X-autosome translocation are generally infertile, regardless of the position of the breakpoint on the X chromosome while the pathogenicity of Xp22.3 subtelomeric duplications is under debate. To shed light into this controversy, we present a rare case, of an azoospermic male with no other significant clinical findings, in whom classical cytogenetics revealed additional unbalanced chromosomal material, at the telomere of the long arm of one homolog of chromosome 9. Read More

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http://dx.doi.org/10.1007/s10815-019-01405-0DOI Listing
January 2019
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A comparison of morphokinetic markers predicting blastocyst formation and implantation potential from two large clinical data sets.

J Assist Reprod Genet 2019 Jan 22. Epub 2019 Jan 22.

IVIRMA Valencia, IVF, Valencia, Spain.

Purpose: To demonstrate whether the standard morphokinetic markers used for embryo selection have a similar relationship to blastocyst formation and implantation in two large clinical data sets.

Methods: This is a retrospective cohort analysis striving to answer two distinct questions utilizing data sets from two large IVF clinics. Blastocysts (BL) and implanted blastocysts (I) in both clinics, IVI-Valencia (BL = 11,414, I = 479) and WMC (BL = 15,902; I = 337), were cultured in a time-lapse system (EmbryoScope, Vitrolife, Sweden). Read More

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http://dx.doi.org/10.1007/s10815-018-1396-xDOI Listing
January 2019
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Morbid obesity-related changes in the expression of lipid receptors, transporters, and HSL in human sperm.

J Assist Reprod Genet 2019 Jan 18. Epub 2019 Jan 18.

Servicio de Bioquímica-Investigación, Madrid, Spain.

Objective: To study the location and expression of receptors (SR-BI/CLA-1, SR-BII, and LDLr) and transporter (ABCA1) involved in uptake and efflux of cholesterol in human spermatozoa and assess whether obesity alters its location/expression and whether this could be related to infertility.

Design: Observational study.

Setting: None PATIENT(S): Ten controls and 20 obese patients. Read More

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http://link.springer.com/10.1007/s10815-019-01406-z
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http://dx.doi.org/10.1007/s10815-019-01406-zDOI Listing
January 2019
7 Reads

Women's morbid conditions are associated with decreased odds of live birth in the first IVF/ICSI treatment: a retrospective single-center study.

J Assist Reprod Genet 2019 Jan 17. Epub 2019 Jan 17.

Department of Pediatrics, Obstetrics and Gynecology, Faculty of Medicine, University of Valencia, Valencia, Spain.

Purpose: The present study aims to ascertain whether there is a causal relationship between women's disease conditions present at the starting time of the first intended oocyte retrieval cycle and IVF/ICSI outcomes, primarily odds of live birth in the first IVF/ICSI treatment.

Methods: This is a retrospective study of infertile healthy and diseased women that had a live birth and/or exhibited a complete first oocyte retrieval cycle. Generalized Estimating Equations (GEE) models were applied to adjust standard errors for the potential correlation among women exhibiting the same infertility etiology. Read More

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http://dx.doi.org/10.1007/s10815-019-01401-4DOI Listing
January 2019
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Pregnancy rates after pre-implantation genetic screening for aneuploidy are only superior when trophectoderm biopsy is performed on hatching embryos.

J Assist Reprod Genet 2019 Jan 15. Epub 2019 Jan 15.

Obstetrics and Gynecology, Staten Island University Hospital, 475 Seaview Avenue, Staten Island, NY, 10305, USA.

Purpose: In vitro fertilization with trophectoderm embryo biopsy and pre-implantation genetic screening with comprehensive chromosomal screening (PGS-CCS) for aneuploidy is becoming increasingly more popular. Embryos are cryopreserved and implanted in a subsequent frozen thawed embryo transfer cycle (FET). No studies have investigated differences in pregnancy outcomes by timing of trophectoderm biopsy relative to stages of blastocyst development. Read More

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http://dx.doi.org/10.1007/s10815-019-01400-5DOI Listing
January 2019
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Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes.

J Assist Reprod Genet 2019 Mar 12;36(3):445-452. Epub 2019 Jan 12.

Faculty of Medicine, Department of Histology and Embryology, Masaryk University, Kamenice 3, 625 00, Brno, Czech Republic.

Propose: The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI).

Methods: The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. Read More

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http://link.springer.com/10.1007/s10815-018-1393-0
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http://dx.doi.org/10.1007/s10815-018-1393-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439061PMC
March 2019
12 Reads

Cumulus-corona gene expression analysis combined with morphological embryo scoring in single embryo transfer cycles increases live birth after fresh transfer and decreases time to pregnancy.

J Assist Reprod Genet 2019 Mar 9;36(3):433-443. Epub 2019 Jan 9.

Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium.

Purpose: Clinical pregnancy rate after IVF with eSET stagnates between 30 and 40%. In order to increase pregnancy and live birth rates, multiple embryo transfer is still common practice. Providing additional non-invasive tools to choose the competent embryo for transfer could avoid multiple pregnancy and improve time to pregnancy. Read More

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http://dx.doi.org/10.1007/s10815-018-01398-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439096PMC

In assisted reproduction by IVF or ICSI, the rate at which embryos develop to the blastocyst stage is influenced by the fertilization method used: a split IVF/ICSI study.

J Assist Reprod Genet 2019 Jan 9. Epub 2019 Jan 9.

Centre for Reproductive and Genetic Health, London, UK.

Purpose: To compare in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI) in regard to post-fertilization development and outcome with the purpose of ascertaining the most effective fertilization method for assisted reproduction.

Methods: A retrospective cohort study of 136 split IVF/ICSI cycles (where sibling oocytes are fertilized by two different methods using the same sperm sample).

Results: IVF-derived embryos developed to the blastocyst stage at a significantly faster rate than ICSI-derived embryos. Read More

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http://link.springer.com/10.1007/s10815-018-1358-3
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http://dx.doi.org/10.1007/s10815-018-1358-3DOI Listing
January 2019
18 Reads

Single best euploid versus single best unknown-ploidy blastocyst frozen embryo transfers: a randomized controlled trial.

J Assist Reprod Genet 2019 Jan 7. Epub 2019 Jan 7.

Antalya IVF, Halide Edip Cd. No:7, Kanal Mh, 07080, Antalya, Turkey.

Purpose: This paper aims to investigate the efficacy of IVF with preimplantation genetic testing for aneuploidy (PGT-A), using only best-scoring blastocysts from young (≤ 35 years) infertile patients undergoing single blastocyst frozen embryo transfers (FET).

Method: In this randomized controlled trial (RCT) registered 29 March 2017, 302 infertile patient-couples eligible to participate underwent autologous ICSI blastocyst freeze-all cycles. Two-hundred and twenty patient-couples satisfied the inclusion criteria (i. Read More

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http://dx.doi.org/10.1007/s10815-018-01399-1DOI Listing
January 2019
1 Read