3,364 results match your criteria Journal of Applied Toxicology [Journal]


Cytotoxic and proinflammatory responses induced by ZnO nanoparticles in in vitro intestinal barrier.

J Appl Toxicol 2019 Apr 24. Epub 2019 Apr 24.

Department of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, via Celoria 10, I-20133, Milan, Italy.

ZnO nanoparticles (NPs) are widely used nowadays, thus the gastrointestinal exposure to ZnO NPs is likely to be relevant and the effects on the intestinal barrier should be investigated. Polarized Caco-2 cells were exposed from the apical (Ap) and basolateral (Bl) compartments to increasing concentrations (0, 10, 50 and 100 μg/mL) of sonochemical (sono) and commercial ZnO NPs. The transepithelial electrical resistance (TEER), cell viability, proinflammatory cytokine release and presence of protein oxidative damage were evaluated after exposure. Read More

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http://dx.doi.org/10.1002/jat.3800DOI Listing

Growth-promoting effects of low-level butyl benzyl phthalate exposure on human neuroblastoma SH-SY5Y cells.

J Appl Toxicol 2019 Apr 23. Epub 2019 Apr 23.

Department of Health Toxicology, MOE Key Laboratory of Environmental and Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.

The purpose of present study was to investigate the impact of butyl benzyl phthalate (BBP) on SH-SY5Y neuroblastoma cells in vitro. The cell counting kit-8 was used to measure cell proliferation and flow cytometry was utilized to study cell cycle phases and apoptosis. Western blotting and quantitative real-time polymerase chain reaction were used to detect levels of aromatase, estrogen receptors (ERs) and some apoptosis and cell cycle-related genes. Read More

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http://dx.doi.org/10.1002/jat.3803DOI Listing

In silico prediction of drug-induced rhabdomyolysis with machine-learning models and structural alerts.

J Appl Toxicol 2019 Apr 21. Epub 2019 Apr 21.

Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.

Drug-induced rhabdomyolysis (DIR) is a serious adverse reaction and can be fatal. In the present study, we focused on the modeling and understanding of the molecular basis of DIR of small molecule drugs. A series of machine-learning models were developed using an Online Chemical Modeling Environment platform with a diverse dataset. Read More

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http://dx.doi.org/10.1002/jat.3808DOI Listing

A novel screening method for transition metal-based anticancer compounds using zebrafish embryo-larval assay and inductively coupled plasma-mass spectrometry analysis.

J Appl Toxicol 2019 Apr 8. Epub 2019 Apr 8.

Environmental and Occupational Health Sciences Institute, Rutgers University, 170 Frelinghuysen Road, Piscataway, NJ, 08854, USA.

As novel metallodrugs continue to emerge, they are evaluated using models, including zebrafish, that offer unique sublethal endpoints. Testing metal-based anticancer compounds with high-throughput zebrafish toxicological assays requires analytical methods with the sensitivity to detect these sublethal tissue doses in very small sample masses (e.g. Read More

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http://dx.doi.org/10.1002/jat.3802DOI Listing
April 2019
4 Reads

Is normalized hindlimb length measurement in assessment of thyroid disruption in the amphibian metamorphosis assay relevant?

J Appl Toxicol 2019 Apr 8. Epub 2019 Apr 8.

Fort Environmental Laboratories, Stillwater, Oklahoma.

The amphibian metamorphosis assay represents an OECD Level 3 and EDSP Tier 1 ecotoxicity test assessing thyroid activity of chemicals in African clawed frog (Xenopus laevis). To evaluate the effectiveness of snout-vent length (SVL) normalization of hindlimb length (HLL), correlation between the HLL and SVL or body weight was evaluated in the control groups of 10 individual studies from three laboratories. Two studies required separate analysis of the Nieuwkoop-Faber (NF) stage ≤60 and >60 animals creating a total of 12 data sets. Read More

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http://dx.doi.org/10.1002/jat.3801DOI Listing
April 2019
2 Reads

The toxic potential of a fourth-generation E-cigarette on human lung cell lines and tissue explants.

J Appl Toxicol 2019 Apr 8. Epub 2019 Apr 8.

Karolinska Institutet, Department of Clinical Sciences, Division of Cardiology, Danderyd University Hospital, Stockholm, Sweden.

The use of electronic cigarettes (E-cigs) is rapidly increasing. The latest generation of E-cigs is highly customizable, allowing for high heating coil temperatures. The aim of this study was to assess the toxic potential of a fourth-generation E-cig. Read More

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http://dx.doi.org/10.1002/jat.3799DOI Listing
April 2019
9 Reads

Nanosized carbon black exposure induces neural injury: effects on nicotinamide adenine dinucleotide phosphate oxidases and endoplasmic reticulum stress.

J Appl Toxicol 2019 Apr 1. Epub 2019 Apr 1.

Key Lab of Modern Toxicology (NJMU), Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, 211166, China.

Carbon black in ambient air is believed to be the cause of many diseases; however, its potential neural toxicity and the underlying mechanisms remain poorly understood. The present study is to evaluate the toxic effects of carbon black nanoparticles, Printex 90, on the neural cell line PC-12. The study revealed that Printex 90 treatment significantly decreased cell viability, accompanied by an enormous increase in reactive oxygen species generation and a decrease in ATP. Read More

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http://dx.doi.org/10.1002/jat.3796DOI Listing
April 2019
6 Reads

Exposures to the environmental contaminants pentachlorophenol and dichlorodiphenyltrichloroethane increase production of the proinflammatory cytokine, interleukin-1β, in human immune cells.

J Appl Toxicol 2019 Mar 25. Epub 2019 Mar 25.

Department of Chemistry, Tennessee State University, 3500 John A. Merritt Blvd., Nashville, TN, 37209, USA.

Pentachlorophenol (PCP) and dichlorodiphenyltrichloroethane (DDT) are organochlorine environmental contaminants found in human blood at very significant levels (as high as 5 μm for PCP and 260 nm for DDT). Cancers of the blood (lymphoma and myeloma) and kidney as well as others have been associated with exposure to these contaminants. Interleukin (IL)-1β is a proinflammatory cytokine and is involved in stimulating cell proliferation. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jat.3798
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http://dx.doi.org/10.1002/jat.3798DOI Listing
March 2019
5 Reads

Activation of the N-methyl-d-aspartate receptor is involved in glyphosate-induced renal proximal tubule cell apoptosis.

J Appl Toxicol 2019 Mar 25. Epub 2019 Mar 25.

School of Public Health and Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University, Shanghai, 200032, China.

Glyphosate-based herbicides have been used worldwide for decades and have been suggested to induce nephrotoxicity, but the underlying mechanism is not yet clear. In this study, we treated a human renal proximal tubule cell line (HK-2) with glyphosate for 24 hours at concentrations of 0, 20, 40 and 60 μm. Glyphosate was found to reduce cell viability and induce apoptosis and oxidative stress in a dose-dependent manner. Read More

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http://dx.doi.org/10.1002/jat.3795DOI Listing
March 2019
2 Reads

Rebaudioside A administration prevents experimental liver fibrosis: an in vivo and in vitro study of the mechanisms of action involved.

J Appl Toxicol 2019 Mar 18. Epub 2019 Mar 18.

Department of Pharmacology, Cinvestav-IPN, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360, Apartado Postal 14-740, Mexico City, Mexico.

Rebaudioside A (Reb A) is a diterpenoid isolated from the leaves of Stevia rebaudiana (Bertoni) that has been shown to possess pharmacological activity, including anti-inflammatory and antioxidant properties. However, the ability of Reb A to prevent liver injury has not been evaluated. Therefore, we aimed to study the potential of Reb A (20 mg/kg; two times daily intraperitoneally) to prevent liver injury induced by thioacetamide (TAA) administration (200 mg/kg; three times per week intraperitoneally). Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jat.3797
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http://dx.doi.org/10.1002/jat.3797DOI Listing

A microarray-based comparative analysis of gene expression profiles in thyroid glands in amphibian metamorphosis: differences in effects between chemical exposure and food restriction.

J Appl Toxicol 2019 Mar 18. Epub 2019 Mar 18.

Bioscience Research Laboratory, Sumitomo Chemical Co., Ltd., 3-1-98 Kasugade-naka, Konohana-ku, Osaka City, Osaka, 554-8558, Japan.

Tadpoles during metamorphosis are sensitive to chemical exposure as shown in the amphibian metamorphosis assay, which is a method to detect effects of chemicals on the functions of hypothalamus-pituitary-thyroid axis. The present study reports existence of different modes of action between pyriproxyfen (PYR) and 6-propyl-2-thiouracil (PTU) under different feeding conditions based on gene expression profiles (transcriptomics) in the thyroid glands of tadpoles of the African clawed frog, Xenopus laevis. PTU and PYR were exposed to the tadpoles during metamorphosis under normal (fed groups, both of PTU and PRY) and restricted feeding (fasted groups, PTU only) conditions; and effects were compared to control groups. Read More

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http://dx.doi.org/10.1002/jat.3791DOI Listing

Cr(VI)-induced genotoxicity and cell cycle arrest in human osteoblast cell line MG-63.

J Appl Toxicol 2019 Mar 18. Epub 2019 Mar 18.

Department of Biology and CESAM, Laboratory of Biotechnology and Cytomics, University of Aveiro, Aveiro, Portugal.

Occupational environments are major exposure routes to Cr(VI). However, Cr(VI) may also establish in bone tissues by ingestion or through Cr containing orthopaedic prostheses that, due to wear and corrosion, may release metal particles and ions potentially affecting bone tissue. The aim of this work was to evaluate the effects of clinically relevant concentrations of Cr(VI) in human osteoblasts, by integrating genotoxic effects, evaluated by the comet assay and cytokinesis-blocked micronucleus assay (scoring the presence of micronucleus, nucleoplasmic bridges and nuclear division index), with the effects on cell cycle and cell viability. Read More

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http://dx.doi.org/10.1002/jat.3793DOI Listing

Effects of inhibition of hepatic sulfotransferase activity on renal genotoxicity induced by lucidin-3-O-primeveroside.

J Appl Toxicol 2019 Apr 12;39(4):650-657. Epub 2018 Dec 12.

Division of Pathology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa, 210-9501, Japan.

Sulfotransferase 1A (SULT1A) expression is lower in the liver of humans than that of rodents. Therefore, species differences should be taken into consideration when assessing the risk of rodent hepatocarcinogens metabolically activated by SULT1A in humans. Although some renal carcinogens require SULT1A-mediated activation, it is unclear how SULT1A activity in the liver affects renal carcinogens. Read More

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http://dx.doi.org/10.1002/jat.3755DOI Listing

In vitro and in silico hormonal activity studies of di-(2-ethylhexyl)terephthalate, a di-(2-ethylhexyl)phthalate substitute used in medical devices, and its metabolites.

J Appl Toxicol 2019 Mar 8. Epub 2019 Mar 8.

Derttech "Packtox", NUTOX, INSERM U1231, Université Bourgogne Franche-Comté, AgroSup Dijon, 21000, Dijon, France.

Plasticizers added to polyvinylchloride used in medical devices can be released into patients' biological fluids. The substitution of di-(2-ethylhexyl)phthalate (DEHP) by alternative plasticizers is essential but their safety must be demonstrated. DEHP, di-(2-ethylhexyl)terephthalate (DEHT) and their metabolites were investigated using level 2 Organization for Economic Co-operation and Development bioassays to screen for in vitro hormonal changes. Read More

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http://dx.doi.org/10.1002/jat.3792DOI Listing
March 2019
1 Read

Effect of perfluorooctanesulfonate exposure on steroid hormone levels and steroidogenic enzyme activities in juvenile Silurana tropicalis.

J Appl Toxicol 2019 Mar 7. Epub 2019 Mar 7.

S.C. Johnson & Son, Racine, WI, USA.

The impact of the perfluoro-chemical, perfluorooctanesulfonate (PFOS), on gonadal steroidogenesis during sexual differentiation in Silurana tropicalis was examined because of its ubiquity in the environment, bioaccumulative nature and potential to disturb endocrine activity. A partial life cycle study exposing S. tropicalis to varying concentrations of PFOS 0. Read More

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http://dx.doi.org/10.1002/jat.3794DOI Listing

Repeated oral dose toxicity study of nickel oxide nanoparticles in Wistar rats: a histological and biochemical perspective.

J Appl Toxicol 2019 Mar 6. Epub 2019 Mar 6.

Toxicology Lab, Applied Biology Department, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, 500007, India.

Despite the increasing use of nickel oxide (NiO) nanoparticles (NPs), limited information is available on their toxicological effects. Health consequences of 28 days repeated oral exposure to NiO NPs have not been explored thoroughly. Hence, toxicity investigations were performed after 28-day daily exposure in albino Wistar rats with NiO NPs following Organization for Economic Co-operation and Development test guideline 407. Read More

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http://dx.doi.org/10.1002/jat.3790DOI Listing
March 2019
1 Read

An assessment of the impact of multi-route co-exposures on human variability in toxicokinetics: A case study with binary and quaternary mixtures of volatile drinking water contaminants.

J Appl Toxicol 2019 Mar 5. Epub 2019 Mar 5.

Department of Environmental and Occupational Health, ESPUM, IRSPUM, Université de Montréal, Montreal, QC, Canada.

This study aimed to assess the impact of multi-route co-exposures to chemicals on interindividual variability in toxicokinetics. Probabilistic physiologically based pharmacokinetic multi-route interaction models were developed for adults and four younger subpopulations. Drinking water-mediated multi-route exposures were simulated for benzene alone or in co-exposure with toluene, ethylbenzene and m-xylene, for trichloroethylene or vinyl chloride (VC), alone and in mixture. Read More

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http://dx.doi.org/10.1002/jat.3787DOI Listing
March 2019
1 Read

Estrogenic effects of fluorinated diiodine alkanes in MCF-7 cells, H295R cells and zebrafish embryo assays.

J Appl Toxicol 2019 Mar 4. Epub 2019 Mar 4.

National Shanghai Center for New Drug Safety Evaluation and Research, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China.

Fluorinated diiodine alkanes (FDIAs), important industrial intermediates in the synthesis of various perfluorinated compounds, which are distributed widely in wildlife and humans. Recent studies showed that FDIAs had in vitro estrogenic effects. However, to date, little information is available regarding the in vivo estrogenic effects of FDIAs and the mechanisms are unclear. Read More

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http://dx.doi.org/10.1002/jat.3783DOI Listing
March 2019
2.982 Impact Factor

Cell-specific immune-modulation of cadmium on murine macrophages and mast cell lines in vitro.

J Appl Toxicol 2019 Mar 3. Epub 2019 Mar 3.

Division of Toxicology, Wageningen University and Research, Stippeneng 4, 6708WE, Wageningen, The Netherlands.

Toxic trace metals are widespread contaminants that are potentially immunotoxic even at environmentally low exposure levels. They can modulate the immunity to infections, e.g. Read More

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http://dx.doi.org/10.1002/jat.3788DOI Listing
March 2019
5 Reads

Molecular interactions of chlorpyrifos and its environmental degradation products with human sex hormone-binding globulin: an in silico study.

J Appl Toxicol 2019 Feb 27. Epub 2019 Feb 27.

Department of Zoology, Cotton University, Guwahati, 781001, Assam, India.

In recent years, there has been a widespread interest and awareness about health issues posed by endocrine-disrupting chemicals present in the environment. These chemicals, often present in food and many consumer products, can interfere with hormone biosynthesis and metabolism and may result in deviation from normal homeostatic control. Chlorpyrifos (CPF), a major endocrine-disrupting chemical is used worldwide as an agricultural insecticide against a broad spectrum of insect pests in rice cultivation and to control termites. Read More

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http://dx.doi.org/10.1002/jat.3789DOI Listing
February 2019
4 Reads

Cytotoxicity profile of pristine graphene on brain microvascular endothelial cells.

J Appl Toxicol 2019 Feb 19. Epub 2019 Feb 19.

Division of Neurotoxicology, National Center for Toxicological Research, Jefferson, AR, USA.

Graphene-based nanomaterials hold the potential to be used in a wide variety of applications, including biomedical devices. Pristine graphene (PG) is an un-functionalized, defect-free type of graphene that could be used as a material for neural interfacing. However, the neurotoxic effects of PG, particularly to the blood-brain barrier (BBB), have not been fully studied. Read More

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http://doi.wiley.com/10.1002/jat.3786
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http://dx.doi.org/10.1002/jat.3786DOI Listing
February 2019
1 Read

Acute kidney injury model established by systemic glutathione depletion in mice.

J Appl Toxicol 2019 Feb 15. Epub 2019 Feb 15.

Department of Drug Safety Sciences, Division of Clinical Pharmacology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.

Glutathione (GSH) is one of the most extensively studied tripeptides. The roles for GSH in redox signaling, detoxification of xenobiotics and antioxidant defense have been investigated. A drug-induced rhabdomyolysis mouse model was recently established in L-buthionine-(S,R)-sulfoximine (BSO; a GSH synthesis inhibitor)-treated normal mice by co-administration of antibacterial drug and statin. Read More

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http://doi.wiley.com/10.1002/jat.3780
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http://dx.doi.org/10.1002/jat.3780DOI Listing
February 2019
5 Reads

Predicting the cytotoxicity of chemicals using ensemble learning methods and molecular fingerprints.

J Appl Toxicol 2019 Feb 14. Epub 2019 Feb 14.

School of Life Science, Liaoning University, Shenyang, 110036, China.

The prediction of compound cytotoxicity is an important part of the drug discovery process. However, it usually appears as poor predictive performance because the datasets are high-throughput and have a class-imbalance problem. In this study, several strategies of performing a structure-activity relationship study for a cytotoxic endpoint in the AID364 dataset were explored to solve the class-imbalance problem. Read More

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http://dx.doi.org/10.1002/jat.3785DOI Listing
February 2019
3 Reads

In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood.

J Appl Toxicol 2019 Feb 14. Epub 2019 Feb 14.

Toxicology Lab, Applied Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India.

The current study was intended to elucidate the cytotoxicity, genotoxicity ability of nickel oxide (NiO) nanoparticles (NPs) and assessment of preliminary mechanism of the toxicity. Characterization studies showed that NiO-NPs have a particle size of 17.94 (±3. Read More

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http://doi.wiley.com/10.1002/jat.3784
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http://dx.doi.org/10.1002/jat.3784DOI Listing
February 2019
5 Reads

Direct activation of aryl hydrocarbon receptor by benzo[a]pyrene elicits T-helper 2-driven proinflammatory responses in a mouse model of allergic dermatitis.

J Appl Toxicol 2019 Feb 12. Epub 2019 Feb 12.

Toxicology Division, Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 303-0043, Japan.

The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that binds to various environmental chemicals and contributes to numerous toxicological effects. However, the direct effects of AhR on the development of allergic diseases are not fully understood. The main aim of this study was to elucidate the action of AhR in the development of cutaneous allergies. Read More

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http://dx.doi.org/10.1002/jat.3782DOI Listing
February 2019

Effect of chronic exposure to inorganic arsenic on intestinal cells.

J Appl Toxicol 2019 Feb 12. Epub 2019 Feb 12.

Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Calle Agustín Escardino 7, 46980, Paterna, Valencia, Spain.

Chronic exposure to inorganic arsenic (As)-As(III) + As(V)-is associated with type 2 diabetes, vascular diseases and various types of cancer. Although the oral route is the main way of exposure to inorganic As, the adverse gastrointestinal effects produced by chronic exposure are not well documented. The aim of the present study is to evaluate the effect of chronic exposure to As(III) on the intestinal epithelium. Read More

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http://dx.doi.org/10.1002/jat.3778DOI Listing
February 2019

Dietary cadmium exposure and renal dysfunction: A risk estimation.

Authors:
Tomoyuki Kawada

J Appl Toxicol 2019 Feb 12. Epub 2019 Feb 12.

Department of Hygiene and Public Health, Nippon Medical School, Tokyo, 113-8602, Japan.

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http://dx.doi.org/10.1002/jat.3781DOI Listing
February 2019

Benzo fury: A new trend in the drug misuse scene.

J Appl Toxicol 2019 Feb 5. Epub 2019 Feb 5.

UCIBIO, REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, Porto, 4050-313, Portugal.

Benzofurans, also known by users as benzo fury or benzofury, are synthetic phenethylamines and constitute the third most prominent group of new psychoactive substances (NPS). As the use of these substances has been spread as an alternative to the classic illicit psychostimulants, such as amphetamines, their legal status was reviewed, resulting in an utter prohibition of these NPS in many countries worldwide. Herein, the prevalence of abuse, chemistry, biological effects, metabolism, and the potential harms and risky behaviors associated with the abuse of benzofurans are reviewed. Read More

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http://dx.doi.org/10.1002/jat.3774DOI Listing
February 2019
2 Reads

Genotoxic effects of silver nanoparticles with/without coating in human liver HepG2 cells and in mice.

J Appl Toxicol 2019 Jan 31. Epub 2019 Jan 31.

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.

With the rapid expansion of human exposure to silver nanoparticles (AgNPs), the genotoxicity screening is critical to the biosafety evaluation of nanosilver. This study assessed DNA damage and chromosomal aberration in the human hepatoma cell line (HepG2) as well as the effects on the micronucleus of bone marrow in mice induced by 20 nm polyvinylpyrrolidone-coated nanosilver (PVP-AgNPs) and 20 nm bare nanosilver (AgNPs). Our results showed that the two types of AgNPs, in doses of 20-160 μg/mL, could cause genetic toxicological changes on HepG2 cells. Read More

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http://doi.wiley.com/10.1002/jat.3779
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http://dx.doi.org/10.1002/jat.3779DOI Listing
January 2019
21 Reads

Immunohistochemistry of γ-H2AX as a method of early detection of urinary bladder carcinogenicity in mice.

J Appl Toxicol 2019 Jan 31. Epub 2019 Jan 31.

Division of Pathology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, 210-9501, Japan.

Phosphorylated histone H2AX (γ-H2AX) has been demonstrated as a DNA damage marker both in vitro and in vivo. We previously reported the effects of genotoxic carcinogens in the urinary bladder of rats by immunohistochemical analysis of γ-H2AX using samples from 28-day repeated-dose tests. To evaluate the application of γ-H2AX as a biomarker of carcinogenicity in the bladder, we examined species differences in γ-H2AX formation in the urinary bladder of mice. Read More

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http://doi.wiley.com/10.1002/jat.3775
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http://dx.doi.org/10.1002/jat.3775DOI Listing
January 2019
1 Read

Role of iron and oxidative stress in the exacerbation of allergic inflammation in murine lungs caused by urban particulate matter <2.5 μm and desert dust.

J Appl Toxicol 2019 Jan 30. Epub 2019 Jan 30.

Department of Environmental Toxicology, University of California, Davis, CA, 95616, USA.

Simultaneous exposure of lipopolysaccharide (LPS) and urban particulate matter <2.5 μm (PM2.5) or desert dust exacerbated murine asthma. Read More

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http://dx.doi.org/10.1002/jat.3773DOI Listing
January 2019
5 Reads

The neonicotinoids acetamiprid and imidacloprid impair neurogenesis and alter the microglial profile in the hippocampal dentate gyrus of mouse neonates.

J Appl Toxicol 2019 Jan 29. Epub 2019 Jan 29.

Laboratory of Developmental Biology, Department of Life Science, Kindai University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan.

Acetamiprid (ACE) and imidacloprid (IMI) are widely used neonicotinoid pesticides. They bind selectively to insect nicotinic acetylcholine receptors (nAChRs) and are considered non-hazardous to mammals. Few studies have assessed the activation of vertebrate nAChRs and the neurodevelopmental toxicity following in utero or neonatal exposure to neonicotinoids; therefore, we evaluated the effects of ACE or IMI exposure on neurogenesis and microglial profiles in the developing hippocampal dentate gyrus (DG) of mouse neonates. Read More

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http://dx.doi.org/10.1002/jat.3776DOI Listing
January 2019
1 Read

Electronic cigarette liquid exposure induces flavor-dependent osteotoxicity and increases expression of a key bone marker, collagen type I.

J Appl Toxicol 2019 Jan 28. Epub 2019 Jan 28.

Department of Biology, The College of Idaho, 2112 Cleveland Blvd, Caldwell, ID, 83605, USA.

Electronic cigarettes (e-cigarettes) are nicotine delivery devices advertised as a healthier alternative to conventional tobacco products, but their rapid rise in popularity outpaces research on potential health consequences. As conventional tobacco use is a risk factor for osteoporosis, this study examines whether exposure to electronic liquid (e-liquid) used in e-cigarettes affects bone-forming osteoblasts. Human MG-63 and Saos-2 osteoblast-like cells were treated for 48 hours with 0. Read More

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http://dx.doi.org/10.1002/jat.3777DOI Listing
January 2019

In silico prediction of chemical reproductive toxicity using machine learning.

J Appl Toxicol 2019 Jan 27. Epub 2019 Jan 27.

Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.

Reproductive toxicity is an important regulatory endpoint in health hazard assessment. Because the in vivo tests are expensive, time consuming and require a large number of animals, which must be killed, in silico approaches as the alternative strategies have been developed to assess the potential reproductive toxicity (reproductive toxicity) of chemicals. Some prediction models for reproductive toxicity have been developed, but most of them were built only based on one single endpoint such as embryo teratogenicity; therefore, these models may not provide reliable predictions for toxic chemicals with other endpoints, such as sperm reduction or gonadal dysgenesis. Read More

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http://doi.wiley.com/10.1002/jat.3772
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http://dx.doi.org/10.1002/jat.3772DOI Listing
January 2019
2 Reads
2.982 Impact Factor

Exposure to cadmium and mono-(2-ethylhexyl) phthalate induce biochemical changes in rat liver, spleen, lung and kidney as determined by attenuated total reflection-Fourier transform infrared spectroscopy.

J Appl Toxicol 2019 May 25;39(5):783-797. Epub 2019 Jan 25.

Department of Andrology, Shiyan Renmin Hospital, Hubei University of Medicine, Shiyan, China.

Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive analytical technique for biochemical analysis of macromolecular components within tissue samples. Cadmium (Cd) and mono-(2-ethylhexyl) phthalate (MEHP), a primary metabolite of di-(2-ethylhexyl) phthalate, are present ubiquitously in the environment and in organisms, and have adverse impacts on ecosystems and human health. Herein we employed ATR-FTIR analysis to identify biomolecular changes in rat liver, spleen, lung and kidney after prepubertal exposure to Cd and MEHP. Read More

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http://doi.wiley.com/10.1002/jat.3767
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http://dx.doi.org/10.1002/jat.3767DOI Listing
May 2019
2 Reads

Effect of arsenic exposure on early eye development in zebrafish (Danio rerio).

J Appl Toxicol 2019 Jan 22. Epub 2019 Jan 22.

School of Marine Sciences, University of Maine, Orono, ME, USA.

Arsenic is a metalloid that contaminates drinking water supplies worldwide. Owing to concerns for human health, the World Health Organization and the US Environmental Protection Agency have established a safe level in drinking water of ≤10 ppb. Recently, arsenic exposure has also been linked to lower IQ values in children. Read More

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http://dx.doi.org/10.1002/jat.3770DOI Listing
January 2019

Pharmacological inhibition of TLR4/NF-κB with TLR4-IN-C34 attenuated microcystin-leucine arginine toxicity in bovine Sertoli cells.

J Appl Toxicol 2019 Jan 22. Epub 2019 Jan 22.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University Harbin, People's Republic of China.

This study investigated the pharmacological inhibition of the toll-like receptor 4 (TLR4) genes as a measure to attenuate microcystin-LR (MC-LR) reproductive toxicity. Bovine Sertoli cells were pretreated with TLR4-IN-C34 (C34) for 1 hour. Thereafter the pretreated and non-pretreated Sertoli cells were cultured in medium containing 10% heat-activated fetal bovine serum + 80 μg/L MC-LR for 24 hours to assess the ability of TLR4-IN-C34 to attenuate the toxic effects of MC-LR. Read More

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http://dx.doi.org/10.1002/jat.3771DOI Listing
January 2019
3 Reads

Gender difference in hepatic toxicity of titanium dioxide nanoparticles after subchronic oral exposure in Sprague-Dawley rats.

J Appl Toxicol 2019 May 15;39(5):807-819. Epub 2019 Jan 15.

Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing, 100191, China.

Existing literature pointed out that the liver may be the target organ of toxicity induced by titanium dioxide nanoparticles (TiO NPs) via oral exposure. Gender differences in health effects widely exist and relevant toxicological research is important for safety assessment. To explore the gender susceptibility of TiO NP-induced hepatic toxicity and the underlying mechanism, we examined female and male Sprague-Dawley rats administrated with TiO NPs orally at doses of 0, 2, 10 and 50 mg/kg body weight per day for 90 days. Read More

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http://dx.doi.org/10.1002/jat.3769DOI Listing
May 2019
2 Reads

Palmitate enhanced the cytotoxicity of ZnO nanomaterials possibly by promoting endoplasmic reticulum stress.

J Appl Toxicol 2019 May 8;39(5):798-806. Epub 2019 Jan 8.

College of Animal Science, Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps, Tarim University, Xinjiang, People's Republic of China.

We recently synthesized ZnO nanomaterials (denoted as ZnO nanorods [NRs] and Mini-NRs) and suggested that their cytotoxicity could be related with the activation of endoplasmic reticulum (ER) stress apoptosis. However, in a complex biological microenvironment, the ER stress-apoptosis pathway could also be modulated by biological molecules, such as free fatty acids, leading to unpredicted biological effects. In this study, we investigated the combined toxicity of ZnO NRs/Mini-NRs and palmitate (PA) to THP-1 macrophages. Read More

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http://doi.wiley.com/10.1002/jat.3768
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http://dx.doi.org/10.1002/jat.3768DOI Listing
May 2019
10 Reads

Derivation of an occupational exposure limit for diacetyl using dose-response data from a chronic animal inhalation exposure study.

J Appl Toxicol 2019 May 8;39(5):688-701. Epub 2019 Jan 8.

Cardno ChemRisk, Brooklyn, NY, USA.

Occupational exposure limits (OELs) have been previously proposed for diacetyl; however, most of these values are based on worker cohort studies that are known to have several limitations and confounders. In this analysis, an 8 hour time-weighted average (TWA) OEL for diacetyl was derived based on data from a chronic, 2 year animal inhalation study recently released by the US National Toxicology Program. In that study, complete histopathology was conducted on male and female mice and rats exposed to 0, 12. Read More

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http://dx.doi.org/10.1002/jat.3757DOI Listing
May 2019
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Acute and subacute oral toxicity of copper oxide nanoparticles in female albino Wistar rats.

J Appl Toxicol 2019 May 7;39(5):702-716. Epub 2019 Jan 7.

Toxicology Unit, Applied Biology Division, CSIR-Indian Institute of Chemical, Technology, Hyderabad, Telangana, 500007, India.

The extensive use of copper oxide nanoparticles (CuO-NPs) in various industries and their wide range of applications have led to their accumulation in different ecological niches of the environment. This excess exposure raises the concern about its potential toxic effects on various organisms including humans. However, the hazardous potential of CuO-NPs in the literature is elusive, and it is essential to study its toxicity in different biological models. Read More

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http://doi.wiley.com/10.1002/jat.3760
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http://dx.doi.org/10.1002/jat.3760DOI Listing
May 2019
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Zinc oxide nanoparticles induced gene mutation at the HGPRT locus and cell cycle arrest associated with apoptosis in V-79 cells.

J Appl Toxicol 2019 May 7;39(5):735-750. Epub 2019 Jan 7.

CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, India.

In recent years, the large-scale production of ZnO nanoparticles (NPs) for various applications is increasing exponentially and may pose serious health issues when inhaled either during occupational exposure or in consumer settings. The mechanisms underlying the toxicity of NPs have recently been studied intensively. Despite the existing studies, the mutagenicity of ZnO NPs in the eukaryotic system is still unclear. Read More

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http://doi.wiley.com/10.1002/jat.3763
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http://dx.doi.org/10.1002/jat.3763DOI Listing
May 2019
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Single wall and multiwall carbon nanotubes induce different toxicological responses in rat alveolar macrophages.

J Appl Toxicol 2019 May 3;39(5):764-772. Epub 2019 Jan 3.

Toxicology and Molecular Biology, Institute Jean Lamour UMR 7198 du CNRS, Université de Lorraine, F-54000, Nancy, France.

Human exposure to airborne carbon nanotubes (CNT) is increasing because of their applications in different sectors; therefore, they constitute a biological hazard. Consequently, developing studies on CNT toxicity become a necessity. CNTs can have different properties in term of length, size and charge. Read More

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http://dx.doi.org/10.1002/jat.3765DOI Listing
May 2019
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Changes in antioxidant, inflammatory and metabolic markers during 1 week cultivation of human skin explants.

J Appl Toxicol 2019 May 3;39(5):773-782. Epub 2019 Jan 3.

Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

Human skin explant (HSE) seems to be a useful model for dermatological/cosmetic testing. HSE prepared from donor superfluous skin from plastic surgery operations is cheap and easily obtainable compared to reconstructed models. The HSE use, however, may be limited by the degeneration processes during cultivation. Read More

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http://doi.wiley.com/10.1002/jat.3766
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http://dx.doi.org/10.1002/jat.3766DOI Listing
May 2019
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miR-222/GAS5 is involved in DNA damage and cytotoxic effects induced by temozolomide in T98G cell line.

J Appl Toxicol 2019 May 21;39(5):726-734. Epub 2018 Dec 21.

Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangdong Province, Guangzhou, 510515, China.

Temozolomide (TMZ), a therapeutic DNA alkylator that can cause lethal DNA damage in cancer cells, is widely used for the standard chemotherapy against glioblastoma. However, long-term treatment with TMZ often causes drug resistance and poor prognosis, the mechanism of which remains largely unclear. This study aimed to investigate the possible role of miR-222/GAS5 axis on DNA damage and cytotoxic effects induced by TMZ in glioblastoma cells (T98G). Read More

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http://doi.wiley.com/10.1002/jat.3762
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http://dx.doi.org/10.1002/jat.3762DOI Listing
May 2019
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Regulation of NRF2, AP-1 and NF-κB by cigarette smoke exposure in three-dimensional human bronchial epithelial cells.

J Appl Toxicol 2019 May 21;39(5):717-725. Epub 2018 Dec 21.

Laboratory of Cellular Pharmacology, School of Pharmacy, Aichi-Gakuin University, 1-100 Kusumoto, Chikusa, Nagoya, 464-8650, Japan.

Cigarette smoke (CS) is a complex mixture of chemicals and interacts with various physiological processes. We previously reported that nuclear factor erythroid 2-related factor 2 (NRF2) was the most sensitive transcription factor to aqueous CS extract (AqCSE) exposure in monolayer cultured human bronchial epithelial cell lines. Recently, in vitro three-dimensional (3D) culture models have been used to supplement pharmacological and toxicological assessments. Read More

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http://doi.wiley.com/10.1002/jat.3761
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http://dx.doi.org/10.1002/jat.3761DOI Listing
May 2019
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Comprehensive lipid profiling of bleomycin-induced lung injury.

J Appl Toxicol 2019 Apr 18;39(4):658-671. Epub 2018 Dec 18.

Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba-shi, Chiba, Japan.

Drug-induced lung injury is an adverse effect of drug treatment that can result in respiratory failure. Because lipid profiling could provide cutting-edge understanding of the pathophysiology of toxicological responses, we performed lipidomic analyses of drug-induced lung injury. We used a mouse model of bleomycin-induced lung injury and followed the physiological responses at the acute inflammatory (day 2), inflammatory-to-fibrosis (day 7) and fibrosis (day 21) phases. Read More

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http://dx.doi.org/10.1002/jat.3758DOI Listing
April 2019
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Maternal di-(2-ethylhexyl) phthalate exposure alters hepatic insulin signal transduction and glucoregulatory events in rat F male offspring.

J Appl Toxicol 2019 May 18;39(5):751-763. Epub 2018 Dec 18.

Department of Endocrinology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, 600 113, India.

Di-(2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer with endocrine disrupting properties. Its widespread use resulted in constant human exposure including fetal development and postnatal life. Epidemiological and experimental data have shown that DEHP has a negative influence on glucose homeostasis. Read More

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http://doi.wiley.com/10.1002/jat.3764
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http://dx.doi.org/10.1002/jat.3764DOI Listing
May 2019
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Prenatal exposure to PCBs in Cyp1a2 knock-out mice interferes with F fertility, impairs long-term potentiation, reduces acoustic startle and impairs conditioned freezing contextual memory with minimal transgenerational effects.

J Appl Toxicol 2019 Apr 18;39(4):603-621. Epub 2018 Dec 18.

Department of Pediatrics, University of Cincinnati College of Medicine, and Division of Neurology, Cincinnati Children's Research Foundation, Cincinnati, OH, 45229, USA.

Polychlorinated biphenyls (PCBs) are toxic environmental pollutants. Humans are exposed to PCB mixtures via contaminated food or water. PCB exposure causes adverse effects in adults and after exposure in utero. Read More

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http://dx.doi.org/10.1002/jat.3751DOI Listing
April 2019
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Effects of co-exposure of lipopolysaccharide and β-glucan (Zymosan A) in exacerbating murine allergic asthma associated with Asian sand dust.

J Appl Toxicol 2019 Apr 12;39(4):672-684. Epub 2018 Dec 12.

Environmental Chemistry Division, National Institute for Environmental Studies, Ibaraki, 305-8506, Japan.

During the 2000s, Asian sand dust (ASD) was implicated in the increasing prevalence of respiratory disorders, including asthma. We previously demonstrated that a fungus from ASD aerosol exacerbated ovalbumin (OVA)-induced airways inflammation. Exposure to heat-inactivated ASD (H-ASD) and either Zymosan A (ZymA, containing β-glucan) or lipopolysaccharide (LPS) exacerbated allergic airways inflammation in a mouse model, but the effects of co-exposure of LPS and β-glucan are unclear. Read More

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http://dx.doi.org/10.1002/jat.3759DOI Listing
April 2019
1 Read