Shock 2021 Feb 17. Epub 2021 Feb 17.
Department of Surgery and Emory Critical Care Center, Emory University School of Medicine, Atlanta, Georgia, USA Department of Critical Care Medicine, The First Affiliated Hospital, China Medical University, Shenyang, China Department of Emergency and Critical Care Medicine, Chiba University Graduate School of Medicine, Chiba, Japan Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA Department of Pathology and Laboratory Medicine, Emory University Department of Surgery and Emory Transplant Center, Emory University School of Medicine, Atlanta, GA, USA.
Abstract: Sepsis-induced intestinal hyperpermeability is mediated by disruption of the epithelial tight junction, which is closely associated with the peri-junctional actin-myosin ring. Genetic deletion of myosin light chain kinase (MLCK) reverses intestinal hyperpermeability and improves survival in a murine model of intra-abdominal sepsis. In an attempt to determine whether these findings could be translated using a more clinically relevant strategy, this study aimed to determine if pharmacologic inhibition of MLCK using the membrane permeant inhibitor of MLCK (PIK) improved gut barrier function and survival following sepsis. Read More