2,451 results match your criteria Intervirology[Journal]


Human Metapneumovirus: Laboratory Methods for Isolation, Propagation, and Plaque Titration.

Intervirology 2019 Mar 27:1-6. Epub 2019 Mar 27.

Facultad de Odontología, Universidad Nacional de Colombia, Bogotá,

The human metapneumovirus (hMPV) is an important viral agent associated with severe infections of the upper and lower airways, especially in young children and immunosuppressed subjects. Nevertheless, in vitro studies of hMPV are very difficult due to the little knowledge we have on its laboratory manipulation.

Objective: The aim of this study was to isolate and propagate hMPV from patients, and to establish a method to quantify the virus by plaque assay. Read More

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http://dx.doi.org/10.1159/000497309DOI Listing
March 2019
3 Reads
1.773 Impact Factor

Human Papillomavirus in Thai Women and Men with Anogenital Warts.

Intervirology 2018 22;61(5):223-229. Epub 2019 Mar 22.

Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand,

Objective: Anogenital warts are caused by human papillomavirus (HPV). Globally, HPV genotypes 6 and 11 are most often associated with anogenital warts. However, the diversity of HPV genotypes found in patients with genital warts in Thailand is unknown. Read More

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http://dx.doi.org/10.1159/000497351DOI Listing
March 2019
1 Read

Analysis of Protein Expression in Human Cells Cocultured with Porcine Peripheral Blood Mononuclear Cells.

Intervirology 2018 19;61(5):237-246. Epub 2019 Mar 19.

Beijing Key Laboratory of Blood Safety and Supply Technologies, Institute of Health Service and Transfusion Medicine, Academy of Military Medical Sciences, Beijing, China.

Objective: Porcine endogenous retroviruses (PERV) involved in pig to human xenotransplantation have raised great concerns because of their ubiquitous nature in pigs and their ability of infecting human cells in vitro. Although no significant cytopathic effect attributed to PERV was evident on PERV-infected human embryonic kidney 293 (HEK293) cells, we did proteomic analysis to investigate the differences of protein profile in order to further characterize the effect of PERV infection.

Methods: HEK293 cells were cocultured with porcine peripheral blood mononuclear cells (PBMCs). Read More

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http://dx.doi.org/10.1159/000495179DOI Listing
March 2019
1 Read

Evaluation of Acquired HIV Drug Resistance among People Living with HIV Who Have Taken Antiretroviral Therapy for 9-15 Months in 14 Triangular Clinics in Iran, 2015-2016.

Intervirology 2019 Mar 12:1-9. Epub 2019 Mar 12.

Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High-Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran.

Aims: The aim of this study was to evaluate drug resistance patterns among Iranian people living with HIV who have taken antiretroviral therapy for 9-15 months.

Methods: A cross-sectional study was conducted between December 2015 and May 2016. Two hundred fifty-two blood samples were collected from all eligible HIV-infected patients at fourteen healthcare settings, located in major provinces in Iran. Read More

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http://dx.doi.org/10.1159/000497036DOI Listing
March 2019
1.773 Impact Factor

Iterative Excretion of Human Cosaviruses from Different Genotypes Associated with Combined Immunodeficiency Disorder.

Intervirology 2018 6;61(5):247-254. Epub 2019 Feb 6.

WHO Regional Reference Laboratory for Poliomyelitis and Measles, Laboratory of Clinical Virology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia,

Background: Human cosaviruses (HCoSVs) are newly discovered enteric viruses in the Picornaviridae family. They have been described in non-polio acute flaccid paralysis, diarrheal patients, and healthy individuals. They remain rarely documented in immunodeficient patients. Read More

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http://dx.doi.org/10.1159/000495182DOI Listing
February 2019
4 Reads

The HPV16 E1 Carboxyl Domain Provides a Helper Function for Adeno-Associated Virus Replication.

Intervirology 2018 17;61(4):185-192. Epub 2019 Jan 17.

Beijing Friendship Hospital, Capital Medical University, Beijing, China,

Background/aims: Recombinant adeno-associated virus (rAAV) is now in the clinic, yet production of rAAV remains problematic. We previously determined that human papillomavirus type 16 (HPV16) E1 protein boosts rAAV yields and E1 enhances AAV Rep78's replication-related biochemistries. Here, we deletion-mapped the helper domain within E1 to help glean its mechanism of action. Read More

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http://dx.doi.org/10.1159/000495137DOI Listing
January 2019
3 Reads

Antiviral Activity of Synthetic Peptides Derived from Physiological Proteins.

Intervirology 2018 17;61(4):166-173. Epub 2019 Jan 17.

Department of Diagnostic, Clinic and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy,

Background/aims: New antivirals are needed to supplement or replace currently used drugs. The aim of this study was to evaluate the antiviral activity of synthetic peptides derived from physiological proteins.

Methods: Vero cell monolayers were infected with herpes simplex virus 1, vesicular stomatitis virus, adenovirus, and coxsackievirus B5 strains in the presence of different concentrations of the selected peptides and viral yield was determined by plaque reduction assays to evaluate the antiviral activity of the peptides. Read More

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http://dx.doi.org/10.1159/000494354DOI Listing
January 2019
2 Reads

Development of a SYBR Green II Real-Time Polymerase Chain Reaction for the Clinical Detection of the Duck-Origin Goose Parvovirus in China.

Intervirology 2018 17;61(5):230-236. Epub 2019 Jan 17.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China,

Objective: To establish an efficient, convenient and quantitative method for the clinical detection of the duck-origin goose parvovirus.

Method: In the present study, a real-time polymerase chain reaction (PCR) method was established for detecting the duck-origin goose parvovirus using the fluorescent chimeric dye SYBR Green II. Specific primers were designed to target a highly conserved region of the VP3 gene of the duck-origin goose parvovirus. Read More

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http://dx.doi.org/10.1159/000495181DOI Listing
January 2019
3 Reads
1.773 Impact Factor

Viral Exploration of Negative Acute Febrile Cases Observed during Chikungunya Outbreaks in Gabon.

Intervirology 2018 9;61(4):174-184. Epub 2019 Jan 9.

Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon,

Non-malarial febrile illness outbreaks were documented in 2007 and 2010 in Gabon. After investigation, these outbreaks were attributed to the chikungunya and dengue viruses (CHIKV and DENV). However, for more than half of the samples analyzed, the causative agent was not identified. Read More

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http://dx.doi.org/10.1159/000495136DOI Listing
January 2019
10 Reads

Loss of Transfected Human Brain Micro-Vascular Endothelial Cell Integrity during Herpes Simplex Virus Infection.

Intervirology 2018 12;61(4):193-203. Epub 2018 Dec 12.

Faculty of Medicine and Biomedical Sciences, MAHSA University, Saujana Putra Campus, Jenjarum, Malaysia,

Objective: Herpes simplex virus infection through the neuronal route is the most well-studied mode of viral encephalitis that can persists in a human host for a lifetime. However, the involvement of other possible infection mechanisms by the virus remains underexplored. Therefore, this study aims to determine the temporal effects and mechanisms by which the virus breaches the human brain micro-vascular endothelial cells of the blood-brain barrier. Read More

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http://dx.doi.org/10.1159/000495180DOI Listing
December 2018
3 Reads

High Herpesvirus Diversity in Wild Rodent and Shrew Species in Central Africa.

Intervirology 2018 16;61(4):155-165. Epub 2018 Nov 16.

Metabiota, San Francisco, California, USA,

Objective: Herpesviruses belong to a diverse order of large DNA viruses that can cause diseases in humans and animals. With the goal of gathering information about the distribution and diversity of herpesviruses in wild rodent and shrew species in central Africa, animals in Cameroon and the Democratic Republic of the Congo were sampled and tested by PCR for the presence of herpesvirus DNA.

Methods: A broad range PCRs targeting either the Polymerase or the terminase gene were used for virus detection. Read More

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https://www.karger.com/Article/FullText/493796
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http://dx.doi.org/10.1159/000493796DOI Listing
November 2018
20 Reads

High-Throughput Sequencing of Putative Novel microRNAs in Rhesus Monkey Peripheral Blood Mononuclear Cells following EV71 and CA16 Infection.

Intervirology 2018 7;61(3):133-142. Epub 2018 Nov 7.

Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China,

Objectives: Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) remain the major pathogens in hand, foot, and mouth disease (HFMD) cases, but the mechanisms of the different pathogeneses that follow EV71 and CA16 infection remain largely unknown.

Methods: Herein, we utilized microRNA (miRNA) deep sequencing to investigate the roles of novel differentially expressed miRNAs in peripheral blood mononuclear cells (PBMCs) infected with EV71 and CA16.

Results: The results identified 13 novel differentially expressed miRNAs in each group. Read More

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https://www.karger.com/Article/FullText/493798
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http://dx.doi.org/10.1159/000493798DOI Listing
March 2019
22 Reads

A Molecular Epidemiological Investigation of Carriage of the Adeno-Associated Virus in Murine Rodents and House Shrews in China.

Intervirology 2018 7;61(3):143-148. Epub 2018 Nov 7.

Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, China,

Objective: To investigate the prevalence of the adeno-associated virus (AAV) in murine rodents and house shrews in 4 provinces of China.

Methods: A total of 469 murine rodents and 19 house shrews were captured between May 2015 and May 2017. Cap gene of AAV sequences was obtained to evaluate the genetic characteristics of rat AAV. Read More

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https://www.karger.com/Article/FullText/493799
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http://dx.doi.org/10.1159/000493799DOI Listing
March 2019
7 Reads

High Prevalence of HBV Lamivudine-Resistant Mutations in HBV/HIV Co-Infected Patients on Antiretroviral Therapy in the Area with the Highest Prevalence of HIV/HBV Co-Infection in China.

Intervirology 2018 26;61(3):123-132. Epub 2018 Oct 26.

Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Guangxi Key Laboratory for the Prevention and Control of Viral Hepatitis, Nanning,

Objectives: We aimed to determine the prevalence of hepatitis B virus (HBV) drug-resistant mutations in patients co- infected with HBV/human immunodeficiency virus (HIV), including both drug-naïve subjects and those who received antiretroviral therapy (ART) in Guangxi, where the prevalence of HIV/HBV co-infection is highest in China.

Methods: Two hundred and three subjects co-infected with HBV/HIV were recruited, including 123 drug-naïve patients (group 1) and 80 who received ART (group 2). The polymerase gene of HBV in the serum of all study subjects was analysed. Read More

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http://dx.doi.org/10.1159/000493797DOI Listing
March 2019
27 Reads

The Effects of IL10 and NK Cells on the Susceptibility to Mouse Cytomegalovirus in BALB/c Mice despite the Compensation of IFNγ.

Intervirology 2018 18;61(3):111-122. Epub 2018 Oct 18.

Objective: The aim of this study was to determine which factors lead to the susceptibility to mouse cytomegalovirus (MCMV) in the spleens of BALB/c mice.

Methods: BALB/c and C57BL/6 mice were randomly divided into a control group and an infection group and sacrificed on day 0, 1, 3, 7, 14, and 28 postinfection. The cytotoxicity of NK cells was determined by evaluating lactate dehydrogenase contents. Read More

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https://www.karger.com/Article/FullText/493316
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http://dx.doi.org/10.1159/000493316DOI Listing
March 2019
6 Reads

Hepatitis E Virus Genotype 1 and Hepatitis A Virus Dual Infection in Pediatric Patients with a Low Socioeconomic Status from Mexico.

Intervirology 2018 2;61(3):105-110. Epub 2018 Oct 2.

Universidad de Guadalajara, Guadalajara,

Objective: We aimed to detect and characterize hepatitis E virus (HEV) RNA in sera samples from a pediatric population infected with the hepatitis A virus (HAV) exhibiting acute hepatitis and to correlate the infection status with the clinical outcome.

Methods: Seventy-five ELISA-positive samples from children containing anti-HAV and anti-HEV IgM were used to amplify and characterize partial regions within HEV ORF2. A statistical comparison of clinical data between HEV IgM-positive/HEV RNA-positive patients and HEV IgM-positive/HEV RNA-negative patients was performed. Read More

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http://dx.doi.org/10.1159/000492425DOI Listing
March 2019
10 Reads

Hepatitis E Virus Genotype 3 Genomes from RNA-Positive but Serologically Negative Plasma Donors Have CUG as the Start Codon for ORF3.

Intervirology 2018 2;61(2):96-103. Epub 2018 Oct 2.

Ulf Lundahls Foundation, Stockholm, Sweden.

Hepatitis E virus (HEV) is a pathogen that causes hepatitis worldwide. Molecular studies have identified HEV RNA in blood products although its significance is not understood. This study was undertaken to characterize HEV genomes in asymptomatic plasma donors from Sweden and Germany lacking anti-HEV. Read More

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http://dx.doi.org/10.1159/000491926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262685PMC
January 2019
8 Reads

Expression of Duplex shRNAs through a Lentiviral Vector against Cellular and Viral Genes Inflicts Sustained Inhibition of Hepatitis C Virus Replication.

Intervirology 2018 25;61(2):79-91. Epub 2018 Sep 25.

Background: The RNAi-based transient therapeutic approach has been well explored for its potential against the hepatitis V virus (HCV). However, to achieve a sustained virological response, a consistent presence of siRNA is needed and it can be achieved by constitutively expressing shRNAs. In this context, the lentiviral vector has emerged as an attractive tool for shRNA delivery against HCV. Read More

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https://www.karger.com/Article/FullText/492220
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http://dx.doi.org/10.1159/000492220DOI Listing
January 2019
7 Reads
1.773 Impact Factor

Three Main Inducers of Alphacoronavirus Infection of Enterocytes: Sialic Acid, Proteases, and Low pH.

Intervirology 2018 3;61(2):53-63. Epub 2018 Sep 3.

Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are similar coronaviruses, causing diseases characterized by vomiting, diarrhea, and death from severe dehydration in piglets. Thus, they have caused huge losses to the swine-breeding industry worldwide. Nowadays, they are easily transmitted among the continents via vehicles, equipment, and cargo. Read More

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https://www.karger.com/Article/FullText/492424
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http://dx.doi.org/10.1159/000492424DOI Listing
January 2019
10 Reads
1.770 Impact Factor

RNAi Targeting of Human Metapneumovirus P and N Genes Inhibits Viral Growth.

Intervirology 2018 24;61(3):149-154. Epub 2018 Aug 24.

Background/aims: Human metapneumovirus (hMPV) is an important human respiratory pathogen and is implicated in an array of respiratory illnesses, ranging from asymptomatic infection to severe bronchiolitis. Currently, there is no reliable vaccine or specific antiviral therapy for hMPV infection and treatment is supportive. The use of ribonucleic acid interference has the potential to change that with the targeting of essential viral genes via small interfering RNAs (siRNAs) offering the ability to directly and rapidly treat viral infections. Read More

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http://dx.doi.org/10.1159/000491927DOI Listing
March 2019
5 Reads

Isolation and Quantification of Mimivirus-Like and Marseillevirus-Like Viruses from Soil Samples in An Aboriginal (Orang asli) Village in Peninsular Malaysia.

Intervirology 2018 17;61(2):92-95. Epub 2018 Aug 17.

International Medical University, Bukit Jalil, Malaysia.

Background: The giant amoebal viruses of Mimivirus and Marseillevirus are large DNA viruses and have been documented in water, soil, and sewage samples. The trend of discovering these giant amoebal viruses has been increasing throughout Asia with Japan, India, and Saudi Arabia being the latest countries to document the presence of these viruses. To date, there have been no reports of large amoebal viruses being isolated in South East Asia. Read More

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http://dx.doi.org/10.1159/000491602DOI Listing
January 2019
4 Reads

Spontaneous Viral Clearance in Sixteen HIV-Infected Patients with Chronic Hepatitis C.

Intervirology 2018 16;61(2):64-71. Epub 2018 Aug 16.

Department of Community Medicine, Information and Health Decision Sciences (MEDCIDS), Faculty of Medicine, University of Porto, Porto, Portugal.

Background/aims: Spontaneous viral clearance of the chronic hepatitis C virus (HCV) in human immunodeficiency virus (HIV)-infected patients is a rare event. We aimed to identify the clinical, therapeutic, demographic, and laboratory features associated with spontaneous HCV clearance in 16 HIV-infected patients with chronic hepatitis C (CHC, the largest case series, to our knowledge).

Methods: This case series study reports the findings from 16 HIV/HCV coinfected patients with CHC who experienced spontaneous clearance of HCV infection. Read More

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http://dx.doi.org/10.1159/000490056DOI Listing
January 2019
39 Reads

Association of the Aquaporin 3 Gene Polymorphism (rs2231231) with Epstein-Barr Virus-Associated Cancers in China.

Intervirology 2018 15;61(2):72-78. Epub 2018 Aug 15.

Department of Pathogenic Biology, Qingdao University Medical College, Qingdao, China.

Background/aims: Aquaporins are widely expressed in a variety of cancers and were found to play an important role in cell migration, angiogenesis, tumor formation, and tumor development. Thus, they have been proposed as a potential biomarker for some cancers. Accordingly, we analyzed the association between a single-nucleotide polymorphism locus of aquaporin 3 (rs2231231) and Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (EBVaNPC), lymphoma (EBVaL), and gastric carcinoma in China. Read More

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https://www.karger.com/Article/FullText/491601
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http://dx.doi.org/10.1159/000491601DOI Listing
January 2019
18 Reads

Ultraviolet Inactivation of Chikungunya Virus.

Intervirology 2018 26;61(1):36-41. Epub 2018 Jul 26.

Objective: Chikungunya virus (CHIKV) is a rapidly emerging arbovirus causing millions of infections in more than 40 countries. CHIKV is typically a biosafety level 3 pathogen in many countries and handling of CHIKV requires a high standard of laboratory safety settings. Many studies require the whole virus to be handled in a biosafety level 2 setting. Read More

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http://dx.doi.org/10.1159/000490567DOI Listing
December 2018
5 Reads
1.773 Impact Factor

Effect of Human Coronavirus OC43 Structural and Accessory Proteins on the Transcriptional Activation of Antiviral Response Elements.

Intervirology 2018 24;61(1):30-35. Epub 2018 Jul 24.

Objectives: The molecular mechanisms underlying the pathogenesis of human coronavirus OC43 (HCoV-OC43) infection are poorly understood. In this study, we investigated the ability of HCoV-OC43 to antagonize the transcriptional activation of antiviral response elements.

Methods: HCoV-OC43 structural (membrane M and nucleocapsid N) and accessory proteins (ns2a and ns5a) were expressed individually in human embryonic kidney 293 (HEK-293) cells. Read More

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http://dx.doi.org/10.1159/000490566DOI Listing
December 2018
3 Reads

Prevalence and Clinical Profile of Human Salivirus in Children with Acute Gastroenteritis in Northern Italy, 2014-2015.

Intervirology 2018 18;61(1):49-52. Epub 2018 Jul 18.

Citoimmunodiagnostics Laboratory, Department of Public Health and Pediatric Sciences, University of Turin Medical School, Turin, Italy.

Objective: Human Salivirus (SalV) has been associated with gastroenteritis on all continents.

Methods: This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy.

Results: The most commonly detected virus was Norovirus genogroup II (GII) (33. Read More

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http://dx.doi.org/10.1159/000490568DOI Listing
December 2018
10 Reads

NS3 Variability in Hepatitis C Virus Genotype 1A Isolates from Liver Tissue and Serum Samples of Treatment-Naïve Patients with Chronic Hepatitis C.

Intervirology 2018 18;61(1):1-8. Epub 2018 Jul 18.

Division of Clinical and Molecular Hepatology, University Hospital of Messina, Messina, Italy.

Background: Hepatitis C virus (HCV) NS3 resistance-associated substitutions (RASs) reduce HCV susceptibility to protease inhibitors. Little is known about NS3 RASs in viral isolates from the liver of chronic hepatitis C (CHC) patients infected with HCV genotype-1a (G1a).

Aim: The objective of this work was to study NS3 variability in isolates from the serum and liver of HCV-G1a-infected patients naïve to direct-acting antivirals (DAAs). Read More

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http://dx.doi.org/10.1159/000489307DOI Listing
December 2018
36 Reads

Molecular Characterization of a New G (VP7) Genotype in Group B Porcine Rotavirus.

Intervirology 2018 16;61(1):42-48. Epub 2018 Jul 16.

Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil.

Rotaviruses (RVs), a common cause of viral gastroenteritis in humans and animals, are classified into 9 established groups/species (RVA-RVI). Although RVB has been found in several countries, genetic variation among RVB field strains remains poorly characterized. RVB strains can be classified into G genotypes based on a nucleotide (nt) homology that exceeds a cutoff value of 80% for the gene that encodes the structural protein VP7. Read More

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http://dx.doi.org/10.1159/000490388DOI Listing
December 2018
5 Reads

Parthenolide Influences Herpes simplex virus 1 Replication in vitro.

Intervirology 2018 12;61(1):14-22. Epub 2018 Jul 12.

Postgraduate Program in Pharmaceutical Sciences, State University of Maringá, Maringá, Brazil.

Background/aims: Parthenolide is a sesquiterpene lactone that is present in plants of the Tanacetum genus, for which many biological effects have already been reported, including antiherpetic activity. Although the effectiveness of parthenolide against Herpes simplex virus 1 (HSV-1) has already been demonstrated, such findings are still controversial. The objective of this study was to investigate the ways in which parthenolide exerts anti-HSV-1 activity. Read More

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http://dx.doi.org/10.1159/000490055DOI Listing
December 2018
22 Reads

Is HERV-K and HERV-W Expression Regulated by miR-155 in Kidney Transplant Patients with Human Cytomegalovirus Infection?

Intervirology 2018 10;61(1):23-29. Epub 2018 Jul 10.

Laboratory of Molecular Virology, Maggiore della Carità Hospital, Novara, Italy.

According to the latest update, 2,578 unique mature micro-RNAs (miRNAs) are currently annotated in the human genome and participate in the regulation of multiple events, such as cellular proliferation or apoptosis. A previous study analyzing global miRNA expression patterns in GH cells (high human endogenous retrovirus, HERV, K vs. low) showed that 2 miRNAs (miR-663 and miR-638) are differentially regulated and exhibit expression parallel to that of HERV-K. Read More

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http://dx.doi.org/10.1159/000490057DOI Listing
December 2018
4 Reads

Epidemiology and Genetic Diversity of Rotavirus in Kunming, China, in 2015.

Authors:
Yongbo Kang Yue Cai

Intervirology 2018 5;61(1):9-13. Epub 2018 Jul 5.

Medical Faculty, Kunming University of Science and Technology, Kunming, China.

Group A rotavirus (RVA) is a serious public problem in China, with a prevalence of 29.7% in diarrhea cases under 5 years of age. A total of 1,224 fecal specimens were collected in 2015 from children under 5 years of age with acute gastroenteritis in Kunming, China, for detection and characterization of rotavirus. Read More

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http://dx.doi.org/10.1159/000489309DOI Listing
December 2018
6 Reads

Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line.

Intervirology 2017 28;60(6):247-262. Epub 2018 Jun 28.

Centre for Research in Medical Entomology, Madurai, India.

Aim: The study was designed to identify putative Chikungunya virus (CHIKV) receptor/s on C6/36 cells that facilitate viral entry.

Methods: The virus overlay protein binding assay (VOPBA) was adopted to identify CHIKV-interacting bands present in C6/36 cell membrane and identity of the protein was established by mass spectrometry. The role of this protein as a putative CHIKV receptor on C6/36 cells was confirmed by infection inhibition assay. Read More

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http://dx.doi.org/10.1159/000489308DOI Listing
June 2018
3 Reads

Genetic Variability in E6 and E7 Oncogenes from Human Papillomavirus Type 58 in Mexican Women.

Intervirology 2017 26;60(6):235-246. Epub 2018 Jun 26.

Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi," Laboratorio de Virología, Mérida, Mexico.

Background/aims: The study aimed to describe human papillomavirus (HPV) 58 genetic variability in E6 and E7 oncogenes from women in southeast Mexico and their phylogenetic relationships with the sequences from other geographical regions.

Methods: The E6-E7 region was amplified by nested PCR, and sequenced for identification of polymorphisms, phylogenetic trees construction, and haplotype and fixation tests.

Results: HPV58 positive samples were obtained from a repository, 54 were amplified, 47 sequences for the E6 gene, and 51 sequences for the E7 gene were obtained. Read More

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http://dx.doi.org/10.1159/000489306DOI Listing
June 2018
3 Reads

Changes in Hemagglutinin and Neuraminidase Genes of pH1N1 Influenza Virus Strains Collected from a North Indian Tertiary Care Hospital during 2015.

Intervirology 2017 26;60(6):263-270. Epub 2018 Jun 26.

Objective: The study was conducted to analyze changes in HA and NA genes of pandemic pH1N1 strains, collected from North Indian tertiary care hospital during 2015 and studied the nucleotide change since 2009. Differences in clinical features of cases positive and negative for influenza viruses were also studied.

Methods: All samples referred for H1N1 testing, were tested for influenza viruses. Read More

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http://dx.doi.org/10.1159/000489632DOI Listing
June 2018
1 Read

Coxsackievirus A24 Variant Associated with Acute Haemorrhagic Conjunctivitis Cases, French Guiana, 2017.

Intervirology 2017 13;60(6):271-275. Epub 2018 Jun 13.

Institut Pasteur, Unité de Biologie des Virus Entériques, INSERM U994, Paris, France.

In 2017, numerous cases of acute haemorrhagic conjunctivitis (AHC) were reported in the Caribbean and in South America. Preliminary reports identified adenoviruses and enteroviruses in some patient samples but, until now, none of the etiologic agents have been fully characterized. We report the full-length genomic sequences of 4 coxsackievirus A24 (CV-A24) isolates collected from AHC patients in French Guiana during this outbreak (May and June 2017). Read More

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http://dx.doi.org/10.1159/000489339DOI Listing
June 2018
5 Reads

Membrane-Associated Kaposi Sarcoma-Associated Herpesvirus Glycoprotein B Promotes Cell Adhesion and Inhibits Migration of Cells via Upregulating IL-1β and TNF-α.

Intervirology 2017 29;60(6):217-226. Epub 2018 Mar 29.

Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina, USA.

Objectives: Kaposi sarcoma-associated herpesvirus (KSHV) glycoprotein B (gB) is expressed on the viral envelope as well as on the cytoplasmic membrane of infected cells. In the current study, we aimed to decipher the impact of membrane-associated gB on adhesion and migration of cells via modulating the expression of cytokines.

Methods: A combination of polymerase chain reaction array, cell adhesion assay, and wound-healing migration assay was conducted to study the influence of the gB-induced cytokines on cell adhesion and migration. Read More

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http://dx.doi.org/10.1159/000487596DOI Listing
March 2018
2 Reads

Zika virus Pathogenesis in Infant Mice after Natural Transmission by the Bite of Infected Mosquitoes.

Intervirology 2017 29;60(6):227-234. Epub 2018 Mar 29.

Microbial Containment Complex, National Institute of Virology, Pune, India.

Objectives: The objective of this study was to understand natural disease progression in infant CD1 mice after the bite of Aedes aegypti mosquitoes infected by the Zika virus (ZIKV, MR-766 strain).

Methods: A. aegypti mosquitoes were experimentally infected with ZIKV MR-766 strain via the oral feeding route. Read More

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http://dx.doi.org/10.1159/000486841DOI Listing
March 2018
5 Reads
1.770 Impact Factor

Development of an Immunoassay for Detection of Torque Teno Virus (TTV) Antibodies Using the N22 Expression Product from TTV Genotype 2.

Intervirology 2017 27;60(5):207-216. Epub 2018 Mar 27.

Aims: This study describes an immunoassay to detect anti-torque teno virus (TTV) antibodies using a peptide obtained from expression of the N22 region of TTV genotype 2.

Methods: The N22 region (∼500 bp) of TTV genotype 2 was cloned in a pET-28a(+) vector and expressed in ZYM-5052 autoinduction medium. Following metal affinity chromatography, a purified polypeptide was used as an antigen for the development of an immunoassay to detect anti-TTV antibodies in human sera. Read More

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http://dx.doi.org/10.1159/000487481DOI Listing
September 2018
8 Reads
1.770 Impact Factor

Genetic Variability and Sequence Relatedness of Matrix Protein in Viruses of the Families Paramyxoviridae and Pneumoviridae.

Intervirology 2017 6;60(5):181-189. Epub 2018 Mar 6.

Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Zagreb, Croatia.

Background: The families Paramyxoviridae and Pneumoviridae comprise a broad spectrum of viral pathogens that affect human health. The matrix (M) protein of these viruses has a central role in their life cycle. In line with this, molecular characteristics of the M proteins from variable viruses that circulated in Croatia were investigated. Read More

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http://dx.doi.org/10.1159/000487049DOI Listing
September 2018
8 Reads

Establishment of a Competitive Binding Assay Identifying the Different Characteristics of Neutralizing Epitopes of Hepatitis E Virus.

Intervirology 2017 6;60(5):190-195. Epub 2018 Mar 6.

Department of Microbiology and Immunology, School of Medicine, Southeast University, Nanjing, China.

Aims: To confirm the different characteristics of genotype-specific and common neutralizing epitopes of hepatitis E virus (HEV).

Methods: A competitive binding assay was established with known genotype-common neutralizing monoclonal antibodies (mAbs) 3G1 and 5G5 as well as genotype-specific neutralizing mAbs 2B1 and 4C5. HEV ORF2 recombinant p166W01 derived from genotype 1 and p166Chn derived from genotype 4 were used as coated antigens, to determine whether the mAbs recognize independent, similar, or overlapping epitopes. Read More

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http://dx.doi.org/10.1159/000487050DOI Listing
September 2018
9 Reads

Aichivirus in Children with Diarrhea in Northern Italy.

Intervirology 2017 2;60(5):196-200. Epub 2018 Mar 2.

Department of Public Health and Pediatric Sciences, University of Turin Medical School, Turin, Italy.

Objective: Since its discovery, Aichivirus (AiV) A has been detected, with an incidence of 0.9-4.1%, primarily when studying outbreaks of diarrhea in children or young adults. Read More

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http://dx.doi.org/10.1159/000487051DOI Listing
September 2018
4 Reads

Detection of Genotype 1a and 1f of Hepatitis E Virus in Patients Treated at Tertiary Care Hospitals in Central India.

Intervirology 2017 1;60(5):201-206. Epub 2018 Mar 1.

Background And Aims: An acute hepatitis, caused by hepatitis E virus (HEV), is a significant global health concern, especially in developing countries. HEV has 1 serotype and 8 genotypes, which are further divided into subtypes. Despite the availability of sporadic cases and outbreak data from India, specific information regarding HEV epidemiological data is lacking from central India. Read More

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http://dx.doi.org/10.1159/000487052DOI Listing
September 2018
3 Reads

Elevated HPV16 E1 Expression Is Associated with Cervical Cancer Progression.

Intervirology 2017 1;60(5):171-180. Epub 2018 Mar 1.

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Objectives: The primary replication protein, HPV E1, has been shown to play a role in mitigating host defence and disrupting normal cell cycle processes, leading to the development of cancer. This study investigated the expression profile of HPV16 E1 in various stages of cervical cancer development and the factors that control E1 expression.

Methods: One hundred and twenty-four HPV16-positive cervical samples ranging from normal to CIN 1, CIN 2/3, and SCC lesions were studied. Read More

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https://www.karger.com/Article/FullText/487048
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http://dx.doi.org/10.1159/000487048DOI Listing
September 2018
7 Reads

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq.

Intervirology 2017 9;60(4):156-164. Epub 2018 Feb 9.

Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University, Baghdad, Iraq.

Objective: This study aims to provide a molecular and epidemiological characterization of bovine papillomavirus (BPV) infections in Iraq.

Methods: The present study focuses on identifying BPV based on clinical and epidemiological manifestations, histopathological examinations, and polymerase chain reactions (PCR). Samples were collected from 163 animals suffering from cutaneous bovine papillomatosis, including 129 females (79. Read More

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http://dx.doi.org/10.1159/000486594DOI Listing
August 2018
7 Reads
1.773 Impact Factor

Cytomegalovirus Glycoprotein B Genotype Distribution in Italian Transplant Patients.

Intervirology 2017 2;60(4):165-170. Epub 2018 Feb 2.

Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy.

Background: The cytomegalovirus (CMV) UL55 gene encodes for a glycoprotein implicated in virus pathogenesis. Based on UL55 polymorphism, CMV has been divided into 4 genotypes. Previous studies investigated the possible role of genotypes in the clinical outcome of infection in different categories of patients; however, few data are available, particularly in the transplant setting and Italian case records. Read More

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http://dx.doi.org/10.1159/000486593DOI Listing
August 2018
5 Reads

Type-Specific Human Papillomavirus Prevalence in Iranian Women with Normal Cervical Cytology: The Impact of Current HPV Vaccines.

Intervirology 2017 25;60(4):125-130. Epub 2018 Jan 25.

Objective: Human papillomavirus (HPV) vaccination is currently not included in the national vaccination program in Iran. Regional data on the distribution of HPV types in women are important as they can predict the impact of currently available vaccines and help health policy makers to consider all the possibilities with regard to HPV vaccination.

Methods: A total of 1,218 Iranian women with normal cervical cytology were included in this study. Read More

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http://dx.doi.org/10.1159/000485898DOI Listing
August 2018
5 Reads

Survival of Viruses in Water.

Intervirology 2018 10;61(5):214-222. Epub 2018 Jan 10.

Institut Pasteur de Lille, Lille, France.

Water, a frequent vehicle for the transmission of viruses, may permit their survival, but many environmental factors will have an adverse effect on the viral population. Risk evaluation requires identification of these factors and assessment of the inactivation rate of infectious viruses. A higher temperature means a faster reduction of the viral population, as do increased sunlight, higher antimicrobial concentration, or higher oxygen levels. Read More

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http://dx.doi.org/10.1159/000484899DOI Listing
January 2018
3 Reads

Preliminary Characterization of El Tor Vibriophage M4.

Intervirology 2017 6;60(4):149-155. Epub 2018 Jan 6.

Division of Electron Microscopy, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, India.

Aims: The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4).

Methods: We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron microscopy. Read More

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http://dx.doi.org/10.1159/000485835DOI Listing
August 2018
9 Reads

Resistance-Associated Mutations and Polymorphisms among Integrase Inhibitor-Naïve HIV-1 Patients in Kuwait.

Intervirology 2017 7;60(4):131-137. Epub 2017 Dec 7.

Department of Microbiology, Faculty of Medicine, Kuwait University, Safat, Kuwait.

Objectives: Resistance-associated mutations (RAMs) in the integrase of different HIV-1 subtypes were investigated in a cohort of patients never exposed to integrase strand transfer inhibitors (INSTIs).

Methods: The viral RNA was extracted from plasma samples of 53 INSTI-naïve patients, and the integrase genetic region was sequenced and analyzed for subtype assignment and drug resistance.

Results: The median viral load at sampling was 5. Read More

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https://www.karger.com/Article/FullText/484692
Publisher Site
http://dx.doi.org/10.1159/000484692DOI Listing
August 2018
9 Reads