6,791 results match your criteria International journal of molecular medicine[Journal]


Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells.

Int J Mol Med 2019 Mar 22. Epub 2019 Mar 22.

Department of Food Science and Technology and Research Institute for Basic Science, Hoseo University, Asan, Chungnam 31499, Republic of Korea.

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic disorders, including atopic dermatitis and asthma. Ursolic acid (UA) has various pharmacological properties, such as antioxidant, anti‑inflammatory and anticancer. However, the effect of UA on TSLP regulation has not been fully elucidated. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4144DOI Listing

[Corrigendum] NLRP3 inflammasome is responsible for Hantavirus inducing interleukin‑1β in THP‑1 cells.

Int J Mol Med 2019 Apr 1. Epub 2019 Apr 1.

Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.

Subsequently to the publication of the above article, the authors have realized that an error was introduced in the preparation of Fig. 2B for publication. In Fig. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4155DOI Listing
April 2019
2 Reads

Oxaliplatin aggravates hepatic oxidative stress, inflammation and fibrosis in a non‑alcoholic fatty liver disease mouse model.

Int J Mol Med 2019 Apr 1. Epub 2019 Apr 1.

Department of Experimental Pathology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

Oxaliplatin (OXA)‑based chemotherapy is widely used in the treatment of gastrointestinal tumors; however, it is associated with chemotherapy‑associated liver injury. Whether OXA induces liver injury and aggravates the already existing hepatic oxidative stress, inflammation and fibrosis in non‑alcoholic fatty liver disease (NAFLD), and whether these effects can be alleviated by reduced glutathione (GSH) treatment, remains unclear. In the present study, OXA induced acute liver injury in NAFLD mice. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4154DOI Listing
April 2019
1 Read

Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion.

Int J Mol Med 2019 Mar 29. Epub 2019 Mar 29.

Department of Cardiology, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100070, P.R. China.

The current study investigated the role of sRAGE in the production of IFN‑γ in macrophages with I/R treatment. The number of macrophages in myocardial tissues treated with I/R with or without sRAGE was determined via immunohistochemical staining. Proliferative activity of macrophages was analyzed by a 5‑BrdU incorporation assay. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4152DOI Listing

Salidroside attenuates oxidized low‑density lipoprotein‑induced endothelial cell injury via promotion of the AMPK/SIRT1 pathway.

Int J Mol Med 2019 Apr 1. Epub 2019 Apr 1.

Department of Cardiovascular, Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China.

Oxidized low‑density lipoprotein (ox‑LDL)‑induced endothelial damage contributes to the initiation and pathogenesis of atherosclerosis. Salidroside can alleviate atherosclerosis and attenuate endothelial cell injury induced by ox‑LDL. However, the mechanisms involved in this process are not fully understood. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4153DOI Listing
April 2019
1.880 Impact Factor

Identification of lncRNA and mRNA expression profiles in rat spinal cords at various time‑points following cardiac ischemia/reperfusion.

Int J Mol Med 2019 Mar 29. Epub 2019 Mar 29.

Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

The identification of the expression patterns of long non‑coding RNAs (lncRNAs) and mRNAs in the spinal cord under normal and cardiac ischemia/reperfusion (I/R) conditions is essential for understanding the genetic mechanisms underlying the pathogenesis of cardiac I/R injury. The present study used high‑throughput RNA sequencing to investigate differential gene and lncRNA expression patterns in the spinal cords of rats during I/R‑induced cardiac injury. Male Sprague Dawley rats were assigned to the following groups: i) Control; ii) 2 h (2 h post‑reperfusion); and iii)v0. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4151DOI Listing
March 2019
3 Reads
1.880 Impact Factor

Protein arginine methyltransferase 6 suppresses adipogenic differentiation by repressing peroxisome proliferator‑activated receptor γ activity.

Int J Mol Med 2019 Mar 27. Epub 2019 Mar 27.

Research Institute of Pharmaceutical Sciences, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.

The present study demonstrated that protein arginine methyltransferase 6 (PRMT6) negatively regulates the activity of peroxisome proliferator‑activated receptor γ (PPARγ). The results indicated that the overexpression of PRMT6 inhibited the transactivity of PPARγ and subsequently decreased the expression levels of PPARγ target genes. Contrarily, the depletion or inhibition of PRMT6 increased PPARγ reporter activity and the expression of its target genes. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4147DOI Listing
March 2019
1.880 Impact Factor

Human peripheral blood‑derived exosomes for microRNA delivery.

Int J Mol Med 2019 Mar 28. Epub 2019 Mar 28.

Division of Cardiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.

Exosomes serve important functions in cell‑to‑cell communication and biological functions by serving as a delivery cargo shuttle for various molecules. The application of an improved delivery method for microRNAs (miRNAs/miRs) may enhance their potential as a therapeutic tool in cardiac diseases. Thus, the present study investigated whether human peripheral blood‑derived exosomes may be used as a delivery cargo system for miRNAs, and whether the delivery of miR‑21 using a human peripheral blood derived‑exosome may influence the degree of remodeling following myocardial infarction (MI). Read More

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http://dx.doi.org/10.3892/ijmm.2019.4150DOI Listing

Spleen tyrosine kinase‑induced JNK‑dependent NLRP3 activation is involved in diabetic cardiomyopathy.

Int J Mol Med 2019 Mar 27. Epub 2019 Mar 27.

School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, P.R. China.

Diabetic cardiomyopathy (DCM) is a leading contributor to the increased morbidity and mortality rates associated with diabetes. Persistent inflammation has previously been reported to be involved in the pathogenesis of DCM. However, the exact underlying molecular mechanisms remain to be fully elucidated. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4148DOI Listing
March 2019
3 Reads

Development of finasteride polymer microspheres for systemic application in androgenic alopecia.

Int J Mol Med 2019 Mar 28. Epub 2019 Mar 28.

KNU‑Center for Nonlinear Dynamics, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Science Kyungpook National University, Daegu 41566, Republic of Korea.

The use of finasteride for alleviating hair loss has been investigated, and it has been applied as an oral dose medication. However, due to the inconvenience of daily drug administration over long period of time, novel controllable finasteride delivery has been actively investigated. As a novel method of finasteride delivery, the development of finasteride‑loaded microspheres for subcutaneous administration is becoming increasingly pharmaceutically important. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4149DOI Listing
March 2019
1 Read

FasL on decidual macrophages mediates trophoblast apoptosis: A potential cause of recurrent miscarriage.

Int J Mol Med 2019 Mar 26. Epub 2019 Mar 26.

Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development, Wuhan, Hubei 430060, P.R. China.

Macrophages can induce Fas ligand (FasL)‑mediated apoptosis, and the deregulation of apoptosis is known to be associated with recurrent miscarriage (RM). The aim of the present study was to investigate the possible involvement of FasL in macrophage‑mediated trophoblast apoptosis and its potential role in RM. Human decidual and placental villous tissues were collected from 81 women (21 for the RM group, 26 for the spontaneous abortion group and 34 for the control group) at 7‑9 weeks of gestation. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4146DOI Listing
March 2019
1 Read
1.880 Impact Factor

Musashi 1-positive cells derived from mouse embryonic stem cells treated with LY294002 are prone to differentiate into intestinal epithelial-like tissues.

Int J Mol Med 2019 Mar 26. Epub 2019 Mar 26.

Department of Gastroenterology, The Second Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China.

The majority of Musashi 1 (Msi1)‑positive cells derived from mouse embryonic stem cells (mESCs) are prone to differentiate into neural epithelial‑like cells, and only a small proportion of Msi1‑positive cells differentiate into intestinal epithelial‑like cells. Whether inhibiting the phosphatidylinositol 3‑kinase (PI3K) signaling of mESCs can promote the differentiation of Msi1‑positive cells into intestinal epithelial‑like cells remains to be fully elucidated. In the present study, to inhibit PI3K signaling, mESCs were treated with LY294002. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4145DOI Listing

Downregulation of Keap1 contributes to poor prognosis and Axitinib resistance of renal cell carcinoma via upregulation of Nrf2 expression.

Int J Mol Med 2019 May 14;43(5):2044-2054. Epub 2019 Mar 14.

Department of Dermatology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530007, P.R. China.

Kelch‑like ECH‑associated protein 1 (Keap1)/nuclear factor erythroid 2‑related factor 2 (Nrf2) signaling has a protective effect on normal cells. A number of previous studies demonstrated that Keap1/Nrf2 signaling is associated with drug resistance in numerous tumors. The aim of the present study was to investigate the roles of Keap1 in renal cell carcinoma (RCC) and its effect on sensitivity to chemotherapy. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443340PMC
May 2019
1 Read

4'‑O‑Methylalpinumisoflavone inhibits the activation of onocytes/macrophages to an immunostimulatory phenotype induced by 27‑hydroxycholesterol.

Int J Mol Med 2019 May 18;43(5):2177-2186. Epub 2019 Mar 18.

Department of Pharmacology, Pusan National University, School of Medicine, Yangsan, Gyeongnam 50612, Republic of Korea.

The epidemiological, animal and cell effects of plant metabolites suggest versatile health benefits of flavonoids. However, whether flavonoids affect the deleterious biological activity of oxygenated cholesterol molecules remains to be elucidated. The present study investigated the effects of 4'‑O‑methylalpinumisoflavone (mAI) isolated from Maclura tricuspidata (Cudrania tricuspidata) on the 27‑hydroxycholesterol (27OHChol)‑induced activation of monocytes/macrophages using human THP‑1 cells. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4135DOI Listing
May 2019
1 Read

Activation of FXR by obeticholic acid induces hepatic gene expression of SR-BI through a novel mechanism of transcriptional synergy with the nuclear receptor LXR.

Int J Mol Med 2019 May 18;43(5):1927-1938. Epub 2019 Mar 18.

Department of Veterans Affairs, Palo Alto Health Care System, Palo Alto, CA 94304, USA.

The farnesoid X receptor (FXR) is known to regulate the gene expression of SR‑BI, which mediates plasma high‑density lipoprotein (HDL)‑cholesterol uptake. Our previous study demonstrated that the activation of FXR by obeticholic acid (OCA) lowered plasma HDL‑cholesterol levels and increased the hepatic mRNA and protein expression levels of SR‑BI in hypercholesterolemic hamsters, but not in normolipidemic hamsters, suggesting that dietary cholesterol may be involved in the OCA‑induced transcription of SR‑BI. In the present study, a functional 90‑base‑pair regulatory region was identified in the first intron of the SR‑BI gene of hamster and mouse that contains a FXR response element (IR‑1) and an adjacent liver X receptor (LXR) response element (LXRE). Read More

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http://dx.doi.org/10.3892/ijmm.2019.4136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443341PMC

Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription.

Int J Mol Med 2019 May 20;43(5):2164-2176. Epub 2019 Mar 20.

Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan.

The hypocretin/orexin neuropeptide system coordinates the regulation of various physiological processes. Our previous study reported that a reduction in the expression of pleomorphic adenoma gene‑like 1 (Plagl1), which encodes a C2H2 zinc‑finger transcription factor, occurs in hypocretin neuron‑ablated transgenic mice, suggesting that PLAGL1 is co‑expressed in hypocretin neurons and regulates hypocretin transcription. The present study examined whether canonical prepro‑hypocretin transcription is functionally modulated by PLAGL1. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445593PMC
May 2019
1 Read

Neuroinflammation and adult hippocampal neurogenesis in neuropathic pain and alkyl glycerol ethers treatment in aged mice.

Int J Mol Med 2019 May 21;43(5):2153-2163. Epub 2019 Mar 21.

'A.V. Zhirmunsky National Scientific Center of Marine Biology', Far Eastern Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russia.

Neuropathic pain is a condition characterized by unpleasant sensory and emotional experiences associated with a number of diseases or injuries affecting the sensory system through various mechanisms. In this study, we focused on the impact of chronic neuropathic pain on the microglial state and hippocampal neurogenesis in aged mice. In addition, we examined the effects of alkyl glycerol ethers (AGE) treatment on behavioral parameters, hippocampal neuronal and microglial plasticity in aged C57BL/6 mice with neuropathic pain. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445594PMC
May 2019
1 Read

Peimine suppresses interleukin‑1β‑induced inflammation via MAPK downregulation in chondrocytes.

Int J Mol Med 2019 May 21;43(5):2241-2251. Epub 2019 Mar 21.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

Osteoarthritis (OA) is the most common type of degenerative joint disease and secreted inflammatory molecules serve a pivotal role in it. Peimine has been reported to have anti‑inflammatory activity. In order to investigate the potential therapeutic role of Peimine in OA, mouse articular chondrocytes were treated with IL‑1β and different doses of Peimine in vitro. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4141DOI Listing
May 2019
2 Reads

Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells.

Int J Mol Med 2019 May 19;43(5):2144-2152. Epub 2019 Mar 19.

Department of Analytical and Forensic Medical Toxicology, Sechenov University, Moscow 119991, Russia.

The aim of this study was to investigate the antioxidant and anti‑apoptotic activities, as well as the underlying mechanisms of action, of Scrophularia buergeriana (S. buergeriana) extract (SBE) in glutamate‑induced SH‑SY5Y cell death. The roots of S. Read More

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http://www.spandidos-publications.com/10.3892/ijmm.2019.4139
Publisher Site
http://dx.doi.org/10.3892/ijmm.2019.4139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443351PMC
May 2019
4 Reads

Isobaric tags for relative and absolute quantitation‑based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3 h.

Int J Mol Med 2019 May 19;43(5):1991-2004. Epub 2019 Mar 19.

Department of Cardiology, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China.

Acute myocardial infarction (AMI) is one of the most common and life‑threatening cardiovascular diseases. However, the ability to diagnose AMI within 3 h is currently lacking. The present study aimed to identify the differentially expressed proteins of AMI within 3 h and to investigate novel biomarkers using isobaric tags for relative and absolute quantitation (ITRAQ) technology. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443345PMC
May 2019
1 Read

Molecular interplay between microRNA-130a and PTEN in palmitic acid-mediated impaired function of endothelial progenitor cells: Effects of metformin.

Int J Mol Med 2019 May 20;43(5):2187-2198. Epub 2019 Mar 20.

Department of Endocrinology and Metabolism, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.

Metformin serves an important role in improving the functions of endothelial progenitor cells (EPCs). MicroRNAs (miRNAs), small non‑coding RNAs, have been investigated as significant regulators of EPC vascular functions. The present study investigated the molecular crosstalk between metformin and miRNA‑130a (miR‑130a) in the functions of EPCs exposed to palmitic acid (PA). Read More

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http://dx.doi.org/10.3892/ijmm.2019.4140DOI Listing
May 2019
2 Reads

[Corrigendum] A 44‑gene set constructed for predicting the prognosis of clear cell renal cell carcinoma.

Int J Mol Med 2019 May 19;43(5):2261. Epub 2019 Mar 19.

Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.

Following the publication of this article, the authors have realized that the affiliation for the third author, Feng Liu, was presented incorrectly: Dr Liu is based at the Department of Nephrology at the China‑Japan Union Hospital of Jilin University. Therefore, the author affiliations for this paper should have appeared as follows: Yonggang Wang1, Yao Wang1 and Feng Liu2. Departments of 1Urology and 2Nephrology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443334PMC
May 2019
1 Read

Penehyclidine hydrochloride alleviates lipopolysaccharide‑induced acute lung injury in rats: Potential role of caveolin‑1 expression upregulation.

Int J Mol Med 2019 May 1;43(5):2064-2074. Epub 2019 Mar 1.

Department of Anesthesiology and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.

The aim of the present study was to examine the protective effect of caveolin‑1 (Cav‑1) in the penehyclidine hydrochloride (PHC)‑based inhibition of lipopolysaccharide (LPS)‑induced acute lung injury (ALI) in vivo and in vitro, in addition to the potential underlying mechanisms. In vivo, an ALI rat model was established via intratracheal administration of LPS (5 mg/kg), and PHC (2 mg/kg) was administered 30 min following LPS treatment. In vitro, the Cav‑1 gene was knocked down by small interfering (si)RNA in J774A. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443352PMC

Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells.

Int J Mol Med 2019 May 1;43(5):2055-2063. Epub 2019 Mar 1.

Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, P.R. China.

Hydrogen sulfide (H2S) is an endogenous gaseous signaling molecule that plays important roles in the cardiovascular system. In our previous studies, we demonstrated that H2S regulates lipid metabolism. In the present study, we aimed to explore the mechanisms through which H2S regulates lipid metabolism in HepG2 cells in vitro. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443339PMC
May 2019
1 Read

Sirt1 promotes autophagy and inhibits apoptosis to protect cardiomyocytes from hypoxic stress.

Int J Mol Med 2019 May 6;43(5):2033-2043. Epub 2019 Mar 6.

Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China.

Sirtuin 1 (Sirt1) exerts its cardioprotective effects in various cardiovascular diseases via multiple cellular activities. However, the therapeutic implications of Sirt1 in hypoxic cardiomyocytes and the underlying mechanisms remain elusive. The present study investigated whether Sirt1 regulates autophagy and apoptosis in hypoxic H9C2 cardiomyocytes and in an experimental hypoxic mouse model. Read More

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http://www.spandidos-publications.com/10.3892/ijmm.2019.4125
Publisher Site
http://dx.doi.org/10.3892/ijmm.2019.4125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443335PMC
May 2019
2 Reads

PEDF decreases cardiomyocyte edema during oxygen‑glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1.

Int J Mol Med 2019 May 12;43(5):1979-1990. Epub 2019 Mar 12.

Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China.

Myocardial edema is divided into cellular edema and interstitial edema; however, the dynamic change of cardiomyocyte edema has not been described in detail. Pigment epithelium‑derived factor (PEDF) is known for its protective effects on ischemic cardiomyocytes; however, the association between PEDF and cardiomyocyte edema remains to be fully elucidated. In the present study, rat neonatal left ventricular cardiomyocytes were isolated and treated with oxygen‑glucose deprivation (OGD) and recovery. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445592PMC

Upregulation of microRNA‑590 in rheumatoid arthritis promotes apoptosis of bone cells through transforming growth factor‑β1/phosphoinositide 3‑kinase/Akt signaling.

Int J Mol Med 2019 May 28;43(5):2212-2220. Epub 2019 Feb 28.

Department of Orthopedics and Traumatology, Yuxi Municipal Hospital of Traditional Chinese Medicine, Yuxi, Yunnan 653100, P.R. China.

The aim of the present study was to further define the role of microRNA (miR)‑590 in osteoarthritis (OA) and to investigate the underlying mechanism. In brief, reverse transcription‑quantitative polymerase chain reaction was used to analyze miR‑590 expression in bone tissue samples from rats with OA. Results indicated the expression of miR‑590 was increased. Read More

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http://www.spandidos-publications.com/10.3892/ijmm.2019.4116
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http://dx.doi.org/10.3892/ijmm.2019.4116DOI Listing
May 2019
6 Reads

Single nucleotide polymorphism rs3746444 in miR‑499a affects susceptibility to non‑small cell lung carcinoma by regulating the expression of CD200.

Int J Mol Med 2019 May 5;43(5):2221-2229. Epub 2019 Mar 5.

Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.

The present study evaluated the association between single nucleotide polymorphism (SNP) rs3746444 and the risk of non‑small cell lung carcinoma (NSCLC) in a Chinese population. Computational analyses and luciferase assays were performed to investigate the regulatory relationship between miR‑499a and CD200. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot assays were performed to examine the effect of rs3746444 on the expression of miR‑499a and CD200. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4124DOI Listing

Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway.

Int J Mol Med 2019 May 12;43(5):2133-2143. Epub 2019 Mar 12.

Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine (Natural Medicine) and Translational Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, P.R. China.

Myrica rubra is well known for its delicious taste and high nutritional value. The present study investigated the potential protective effects and mechanisms of M. rubra flavonoids (MRF) extract on isoproterenol (ISO)‑induced myocardial injury in rats and hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443338PMC
May 2019
2 Reads

Potential role of the IL17RC gene in the thoracic ossification of the posterior longitudinal ligament.

Int J Mol Med 2019 May 12;43(5):2005-2014. Epub 2019 Mar 12.

Department of Orthopedics, Xuanwu Hospital of Capital Medical University, Beijing 100053, P.R. China.

The thoracic ossification of the posterior longitudinal ligament (T‑OPLL) can cause thoracic spinal stenosis, which results in intractable myelopathy and radiculopathy. Our previous whole‑genome sequencing study first reported rs199772854 in the interleukin 17 receptor C (IL17RC) gene as a potentially pathogenic loci for T‑OPLL. The aim of the present study was to examine the effects of the IL17RC gene rs199772854A site mutation on osteogenesis by establishing a model of osteogenic differentiation. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443333PMC
May 2019
1 Read

Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice.

Int J Mol Med 2019 May 6;43(5):2103-2117. Epub 2019 Mar 6.

Institute of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.

Family with sequence similarity 20‑member C (FAM20C), a recently characterized Golgi kinase, performs numerous biological functions by phosphorylating more than 100 secreted proteins. However, the role of FAM20C in the salivary glands remains undefined. The present study demonstrated that FAM20C is mainly located in the cytoplasm of duct epithelial cells in the salivary glands. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443332PMC
May 2019
1 Read

LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins.

Int J Mol Med 2019 May 8;43(5):2118-2132. Epub 2019 Mar 8.

Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, Manama 26671, Kingdom of Bahrain.

Type 2 diabetes mellitus (T2DM) is a disease associated with a number of metabolic disturbances, including protein metabolism. In the present study, blood samples were obtained from Bahraini subjects, including 6 patients with T2DM and 6 age‑ and sex‑matched, non‑diabetic, healthy controls. Depleted and non‑depleted sera were prepared from the collected blood, and the global protein expression changes were evaluated by liquid chromatography tandem mass spectrometry. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443330PMC
May 2019
1 Read

GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathway.

Int J Mol Med 2019 May 12;43(5):1939-1950. Epub 2019 Mar 12.

Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

Preeclampsia (PE) is a pathological condition that manifests during pregnancy as the occurrence of an abnormal urine protein level and increased blood pressure due to inadequate cytotrophoblast invasion. To elucidate the mechanism underlying PE, the present study primarily focused on the regulatory effects and mechanism of the G protein γ 7 (GNG7) on placental cytotrophoblasts in a rat PE model. Initially, the PE model was established with 45 specific pathogen‑free Sprague‑Dawley rats (30 females and 15 males). Read More

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http://dx.doi.org/10.3892/ijmm.2019.4129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443336PMC
May 2019
2 Reads

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.

Int J Mol Med 2019 May 5;43(5):2015-2023. Epub 2019 Mar 5.

Department of Medicine, Tianjin Huanhu Hospital, Tianjin 300350, P.R. China.

Barbaloin (Bar) has a myocardial protective effect, but its mechanism of action is uncertain. The endoplasmic reticulum stress (ERS)‑mediated apoptosis pathway serves an important role in the pathogenesis of myocardial ischemia‑reperfusion injury (MIRI). Inhibiting ERS may significantly improve the progression of MIRI and serve a role in its prevention. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443342PMC

Honokiol induces apoptosis and suppresses migration and invasion of ovarian carcinoma cells via AMPK/mTOR signaling pathway.

Int J Mol Med 2019 May 5;43(5):1969-1978. Epub 2019 Mar 5.

Research Institute for Medicinal Sciences, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.

Honokiol, a natural biphenolic compound, exerts anticancer effects through a variety of mechanisms on multiple types of cancer with relatively low toxicity. Adenosine 5'‑phosphate‑activated protein kinase (AMPK), an essential regulator of cellular homeostasis, may control cancer progression. The present study aimed to investigate whether the anticancer activities of honokiol in ovarian cancer cells were mediated through the activation of AMPK. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443331PMC
May 2019
1 Read

Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

Int J Mol Med 2019 May 4;43(5):2086-2102. Epub 2019 Mar 4.

Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, P.R. China.

The purpose of the present study was to investigate whether bone marrow mesenchymal stem cells (BMMSCs) modified by CXC‑chemokine receptor type 3 (CXCR3) and heme oxygenase‑1 (HO‑1) genes can repair damaged intestinal epithelial cells in vitro, and the role of the p38 mitogen‑activated protein kinase (p38‑MAPK) pathway in this process. A model of intestinal epithelial crypt cell line‑6 (IEC‑6) damage was created, and BMMSCs were transfected with either the CXCR3 and/or HO‑1 gene in vitro. There were nine experimental groups in which the damaged IEC‑6 cells were co‑cultured with differentially‑treated BMMSCs and lymphocytes for 24 h. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445595PMC
May 2019
1 Read

[Corrigendum] Antiproliferative effects of imatinib mesylate on ZR‑75‑1 and MDA‑MB‑231 cell lines via PDGFR‑β, PDGF‑BB, c‑Kit and SCF expression.

Int J Mol Med 2019 May 4;43(5):2259. Epub 2019 Mar 4.

Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), 14176‑13151 Tehran, Iran.

An interested reader drew to our attention that the above study appeared to contain a high level of overlap with an article by the same authors published in the journal Drug Design, Development and Therapy [Kadivar A, Kamalidehghan B, Akbari Javar H, Karimi B, Sedghi R and Noordin MI: Antiproliferation effect of imatinib mesylate on MCF7, T‑47D tumorigenic and MCF 10A nontumorigenic breast cell lines via PDGFR‑β, PDGF‑BB, c‑Kit and SCF genes. Drug Des Devel Ther 11: 469‑481, 2017]. Following an internal investigation and also in liaison with the authors, it was established that, although the studies were conducted along broadly similar lines, the papers contained entirely different data involving two different subsets of cell lines; the submission to Drug Des Devel Ther aimed to explore the effects of imatinib mesylate on three different groups, with each group being represented by a cell line, whereas the submission to Int J Mol Med explored the effectiveness of imatinib mesylate in breast cancer cell lines. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443349PMC
May 2019
1.880 Impact Factor

URG11 promotes proliferation and induced apoptosis of LNCaP cells.

Int J Mol Med 2019 May 5;43(5):2075-2085. Epub 2019 Mar 5.

Department of Urology, Tongren Hospital Shanghai Jiao Tong University School of Medicine, Shanghai 200336, P.R. China.

von Willebrand factor C and EGF domain‑containing protein (URG11), a cell growth regulator, is involved in the progression of a variety of types of cancer, including prostate cancer (Pca). However, the functions of the URG11 gene in Pca cells require in‑depth investigation. The mRNA and protein levels of URG11 were measured by reverse transcription quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443344PMC
May 2019
1 Read

Effects of miR‑103a‑3p on the autophagy and apoptosis of cardiomyocytes by regulating Atg5.

Int J Mol Med 2019 05 12;43(5):1951-1960. Epub 2019 Mar 12.

Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, P.R. China.

Autophagy and apoptosis are associated with cardiovascular diseases. Emerging evidence shows that microRNAs (miRs) are critical in the development of pathological processes underlying cardiovascular diseases by regulating the induction of apoptosis and autophagy. The present study aimed to investigate the role of miR‑103a‑3p in cardiomyocyte injury through autophagy and apoptosis. Read More

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http://www.spandidos-publications.com/10.3892/ijmm.2019.4128
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http://dx.doi.org/10.3892/ijmm.2019.4128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443343PMC
May 2019
4 Reads

Induction of cardiomyocyte‑like cells from hair follicle cells in mice.

Int J Mol Med 2019 May 13;43(5):2230-2240. Epub 2019 Mar 13.

Department of Animal Science and Technology, College of Biotechnology and Natural Resources, Chung‑Ang University, Anseong, Gyeonggi‑do 17546, Republic of Korea.

Hair follicles (HFs) are a well‑characterized niche for adult stem cells (SCs), and include epithelial and melanocytic SCs. HF cells are an accessible source of multipotent adult SCs for the generation of the interfollicular epidermis, HF structures and sebaceous glands in addition to the reconstitution of novel HFs in vivo. In the present study, it was demonstrated that HF cells are able to be induced to differentiate into cardiomyocyte‑like cells in vitro under specific conditions. Read More

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http://www.spandidos-publications.com/10.3892/ijmm.2019.4133
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http://dx.doi.org/10.3892/ijmm.2019.4133DOI Listing
May 2019
9 Reads

Enhanced antiproliferative effect of resveratrol in head and neck squamous cell carcinoma using GE11 peptide conjugated liposome.

Int J Mol Med 2019 Apr 13;43(4):1635-1642. Epub 2019 Feb 13.

Shenzhen Key Laboratory for Drug Addiction and Medication Safety, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 510852, P.R. China.

The present study describes the preparation of a dodecapeptide YHWYGYTPQNVI (GE11)‑conjugated liposome bound with polyethylene glycol to enhance the therapeutic effect of resveratrol (RSV) in head and neck cancer cells. The results indicated that (RSV)‑loaded GE11‑conjugated liposomes (RSV‑GL) exhibited a high entrapment efficiency of >95%, with an active drug loading level of 19.5% w/w. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414163PMC
April 2019
3 Reads

Genome editing: A perspective on the application of CRISPR/Cas9 to study human diseases (Review).

Int J Mol Med 2019 Apr 26;43(4):1559-1574. Epub 2019 Feb 26.

Universidad Autónoma de Nuevo León, Department of Biochemistry and Molecular Medicine, School of Medicine and University Hospital 'Dr. José E. González', Monterrey, Nuevo León 64460, México.

Genome editing reemerged in 2012 with the development of CRISPR/Cas9 technology, which is a genetic manipulation tool derived from the defense system of certain bacteria against viruses and plasmids. This method is easy to apply and has been used in a wide variety of experimental models, including cell lines, laboratory animals, plants, and even in human clinical trials. The CRISPR/Cas9 system consists of directing the Cas9 nuclease to create a site‑directed double‑strand DNA break using a small RNA molecule as a guide. Read More

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http://www.spandidos-publications.com/10.3892/ijmm.2019.4112
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http://dx.doi.org/10.3892/ijmm.2019.4112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414166PMC
April 2019
12 Reads
1.880 Impact Factor

Effects of HGF and KGF gene silencing on vascular endothelial growth factor and its receptors in rat ultraviolet radiation‑induced corneal neovascularization.

Int J Mol Med 2019 Apr 27;43(4):1888-1899. Epub 2019 Feb 27.

Department of Otolaryngology, The Second Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

Hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF), two paracrine growth factors, modulate corneal epithelial cell metabolism, apoptosis and survival. Vascular endothelial growth factor (VEGF) serves as a proangiogenic factor in corneal neovascularization (CNV), which is a major cause of vision impairment and corneal blindness. The aim of the present study was to evaluate the ability of HGF and KGF to influence VEGF and its receptor, kinase insert domain receptor (Flk‑1) in corneal injury and CNV in rats induced by ultraviolet radiation (UVR). Read More

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http://dx.doi.org/10.3892/ijmm.2019.4114DOI Listing

Molecular interaction of the antiviral compound CW‑33 and its analogues with the NS2B‑NS3 protease of the Japanese encephalitis virus.

Int J Mol Med 2019 May 27;43(5):2024-2032. Epub 2019 Feb 27.

School of Pharmacy, China Medical University, Taichung 40402, Taiwan, R.O.C.

In a previous study from our group, a novel compound, namely CW‑33 (ethyl 2‑(3',5'‑dimethylanilino)‑​4‑oxo‑4,5‑dihydrofuran‑3‑carboxylate) was identified that exhibited antiviral activity for Japanese encephalitis virus (JEV). The viral NS2B‑NS3 serine protease serves an important role in cytoplasmic cleavage events that occur during viral polyprotein maturation. The inhibition of viral RNA and protein syntheses was responsible for the antiviral activities of the novel furanonaphthoquinone derivatives that were discovered for the prevention of JEV infection. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443346PMC
May 2019
6 Reads

Proteasome activator REGγ promotes inflammation in Leydig cells via IkBε signaling.

Int J Mol Med 2019 May 27;43(5):1961-1968. Epub 2019 Feb 27.

Department of Urology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.

The development of testicular inflammation affects the normal male reproductive function. The proteasome activator complex subunit 3 (REGγ) has been suggested to regulate experimental colitis. However, to the best of our knowledge, a potential association between REGγ and testicular inflammation has not been demonstrated. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443337PMC
May 2019
1.880 Impact Factor

Dimethylglycine sodium salt protects against oxidative damage and mitochondrial dysfunction in the small intestines of mice.

Int J Mol Med 2019 May 11;43(5):2199-2211. Epub 2019 Feb 11.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.

Few studies have investigated the use of dimethylglycine sodium salt (DMG‑Na) to protect against small intestinal damage, despite its prevalence in the treatment of human diseases. The present study aimed to evaluate the protective effects of DMG‑Na against oxidative damage and mitochondrial dysfunction in the small intestines of mice. A total of 100 male Kunming mice were randomly assigned to five groups (n=20 per group): i) Mice gastric intubation with 0. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4093DOI Listing

SIRT1 inhibits apoptosis in in vivo and in vitro models of spinal cord injury via microRNA-494.

Int J Mol Med 2019 Apr 22;43(4):1758-1768. Epub 2019 Feb 22.

Department of Orthopaedics, Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.

The aim of the present study was to investigate the function and mechanism of sirtuin 1 (SIRT1) in spinal cord injury (SCI). Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression levels of microRNA (miR)‑494. MTT assay, lactate dehydrogenase activity assay and flow cytometry were used to analyze the effects of miR‑494 on cell growth and apoptosis in a model of SCI. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414168PMC
April 2019
1.880 Impact Factor

Berberine prevents human nucleus pulposus cells from IL‑1β‑induced extracellular matrix degradation and apoptosis by inhibiting the NF‑κB pathway.

Int J Mol Med 2019 Apr 21;43(4):1679-1686. Epub 2019 Feb 21.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.

Intervertebral disc degeneration (IDD) is widely considered to be one of the main causes of lower back pain, which is a chronic progressive disease closely related to inflammation, nucleus pulposus (NP) cell apoptosis and extracellular matrix (ECM) degradation. Berberine (BBR) is an alkaloid compound with an anti‑inflammatory effect and has been reported to exert therapeutic action in several inflammatory diseases, including osteoarthritis. Therefore, it was hypothesized that BBR may have a therapeutic effect on IDD through inhibition of the inflammatory response. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414164PMC

Tanshinone IIA inhibits lipopolysaccharide‑induced inflammatory responses through the TLR4/TAK1/NF‑κB signaling pathway in vascular smooth muscle cells.

Int J Mol Med 2019 Apr 18;43(4):1847-1858. Epub 2019 Feb 18.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.

To aim of the present study was to determine whether Tanshinone IIA (Tan IIA) inhibits lipopolysaccharide (LPS)‑induced inflammation in vascular smooth muscle cells (VSMCs) from rats and elucidate the underlying molecular mechanism. VSMCs were primarily cultured and then treated with LPS (1 µg/l) and Tan IIA (25, 50 and 100 µmol/l) for 24 h. Monocyte chemoattractant protein (MCP)‑1, interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α levels were detected by ELISA and reverse transcription‑quantitative polymerase chain reaction. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4100DOI Listing

Co‑expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis.

Int J Mol Med 2019 Apr 20;43(4):1687-1698. Epub 2019 Feb 20.

Department of Obstetrics and Gynecology, China Medical University Shengjing Hospital, Shenyang, Liaoning 110004, P.R. China.

CD147 is a highly glycosylated transmembrane protein expressed on the surface of tumor cells. In the present study, the expression and clinical significance of the Lewis y antigen and CD147 in epithelial ovarian cancer (EOC) were analyzed, and the function and correlation in between the expression of Lewis y and CD147 were evaluated using immunohistochemical staining, reverse transcription‑quantitative polymerase chain reaction analysis, immunocytochemical staining, immunoprecipitation and western blotting. The results showed that the expression of CD147 was higher in EOC tissues and correlated with a higher tumor burden. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414171PMC