1,474 results match your criteria International Journal of Laboratory Hematology [Journal]


Clotting and chromogenic factor VIII assay variability in post-infusion and spiked samples containing full-length recombinant FVIII or recombinant factor VIII Fc fusion protein (rFVIIIFc).

Int J Lab Hematol 2018 Dec 17. Epub 2018 Dec 17.

UK NEQAS Blood Coagulation, Sheffield, UK.

Introduction: Variability in FVIII measurement is a recognized problem. There are limited data for samples containing recombinant Factor VIII Fc fusion protein (rFVIIIFc). Many studies use samples for which factor concentrate has been spiked into FVIII deficient plasma in vitro. Read More

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http://dx.doi.org/10.1111/ijlh.12940DOI Listing
December 2018

Eosin-5'-maleimide binding test-Do we use appropriate reference values to detect hereditary spherocytosis in neonates?

Int J Lab Hematol 2018 Dec 10. Epub 2018 Dec 10.

Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland.

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http://dx.doi.org/10.1111/ijlh.12958DOI Listing
December 2018
3 Reads

Evaluation of next-generation sequencing-based clonality analysis of T-cell receptor gamma gene rearrangements based on a new interpretation algorithm.

Int J Lab Hematol 2018 Dec 8. Epub 2018 Dec 8.

Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium.

Introduction: T-cell receptor gene (TRG) rearrangement profiling is an essential component of the workup at diagnosis of T-cell malignancies. TRG amplification by polymerase chain reaction (PCR) and analysis by capillary electrophoresis (PCR-CE) is mostly widely used but is hampered by a subjective interpretation of its results and possible false-positive interpretation of clonality. Several studies evaluated the advantage of TRG rearrangement analysis by Next Generation Sequencing (TRG-NGS), however few have proposed an adequate data interpretation algorithm. Read More

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http://dx.doi.org/10.1111/ijlh.12954DOI Listing
December 2018

Measurement of extended half-life recombinant factor IX products in clinical practice.

Int J Lab Hematol 2018 Dec 7. Epub 2018 Dec 7.

Department Of Coagulation, Royal Hallamshire Hospital, Sheffield, UK.

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http://dx.doi.org/10.1111/ijlh.12953DOI Listing
December 2018

Genetic variants of erythropoietin (EPO) and EPO receptor genes in familial erythrocytosis.

Int J Lab Hematol 2018 Dec 3. Epub 2018 Dec 3.

Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Objectives: Erythrocytosis is characterized by the expansion of erythrocyte compartment including elevated red blood cell number, hematocrit, and hemoglobin content. Familial erythrocytosis (FE) is a congenital disorder with different genetic background. Type 1 FE is primary FE caused by mutation in erythropoietin receptor gene (EPOR). Read More

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http://dx.doi.org/10.1111/ijlh.12949DOI Listing
December 2018
2 Reads

Gaucher disease screening at a general adult hematology tertiary care centre: A prospective study.

Int J Lab Hematol 2018 Dec 4. Epub 2018 Dec 4.

Division of Hematology, Department of Medicine, Western University, London, Ontario, Canada.

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http://dx.doi.org/10.1111/ijlh.12960DOI Listing
December 2018

How I investigate Eosinophilia.

Int J Lab Hematol 2018 Nov 30. Epub 2018 Nov 30.

Medical College of Georgia, Augusta University, Augusta, Georgia.

Eosinophilia is typically secondary, that is, reactive, in nature and is associated with a wide variety of neoplastic and non-neoplastic disorders. Clonal eosinophilia is also seen in a wide variety of hematopoietic neoplasms, and sub-classification can be diagnostically challenging. A proper evaluation of persistent eosinophilia involves correlation of clinical history, laboratory data, cellular morphology, and ancillary testing. Read More

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http://dx.doi.org/10.1111/ijlh.12955DOI Listing
November 2018
1 Read

The phenotypic and molecular diversity of hemoglobinopathies in India: A review of 15 years at a referral center.

Int J Lab Hematol 2018 Nov 29. Epub 2018 Nov 29.

National Institute of Immunohaematology, Mumbai, India.

Introduction: The hemoglobinopathies pose a significant health burden in India. Apart from the β thalassemias and sickle cell disorders, α thalassemias and structural hemoglobin variants are also common. Here we have reviewed the phenotypic and molecular diversity of hemoglobinopathies encountered at a referral center in western India over a period of 15 years. Read More

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http://dx.doi.org/10.1111/ijlh.12948DOI Listing
November 2018
1 Read

Platelet miR-28 expression level and thrombocytosis in MPN patients.

Int J Lab Hematol 2018 Nov 27. Epub 2018 Nov 27.

Department of Health, Krasnoyarsk Branch of the "National Research Center for Hematology", Krasnoyarsk, Russian Federation.

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http://dx.doi.org/10.1111/ijlh.12951DOI Listing
November 2018

Flow cytometry identification of nonhemopoietic neoplasms during routine immunophenotyping.

Int J Lab Hematol 2018 Nov 27. Epub 2018 Nov 27.

Pathology Division, Citta' della Salute e della Scienza Hospital, Turin, Italy.

Introduction: Nonhemopoietic neoplasms (NHNs) may be encountered during routine flow cytometry (FC) immunophenotyping. The clue of their presence mainly relies on detection of CD45-negative (CD45-) cells with altered scatter parameters.

Methods: In this study, we evaluated a monoclonal antibody combination conceived to characterize the CD45- population by FC, suspected of belonging to NHNs, when present. Read More

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http://dx.doi.org/10.1111/ijlh.12946DOI Listing
November 2018

Utility of peripheral blood smear in rapid diagnosis of Pertussis.

Int J Lab Hematol 2018 Nov 27. Epub 2018 Nov 27.

A.O.R.N. "G. Rummo" Benevento, U.O. Patologia Clinica, Benevento, Italy.

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http://dx.doi.org/10.1111/ijlh.12947DOI Listing
November 2018

Influence of DOAC Stop on coagulation assays in samples from patients on rivaroxaban or apixaban.

Int J Lab Hematol 2018 Nov 23. Epub 2018 Nov 23.

Haematology Laboratory, Barts Health NHS Trust, The Royal London Hospital, London, UK.

Introduction: Direct oral anticoagulants (DOACs) require no laboratory monitoring, but they interfere with almost all clotting tests to a varying degree, depending on the DOAC, assay principles and reagents used. DOAC Stop (Haematex Research, Sydney, Australia) has recently been shown to adsorb DOACs from spiked and patient plasmas. The aim of our work was to investigate the DOAC Stop effect on a range of haemostasis assays on plasmas collected from patients on rivaroxaban or apixaban, to see whether it removes the effect of these drugs to enable more accurate interpretation of coagulation assays. Read More

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http://dx.doi.org/10.1111/ijlh.12950DOI Listing
November 2018
21 Reads

Quantitative detection of IKZF1 deletion by digital PCR in patients with acute lymphoblastic leukemia.

Int J Lab Hematol 2018 Nov 20. Epub 2018 Nov 20.

Department of Hematology, Faculty of Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

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http://dx.doi.org/10.1111/ijlh.12945DOI Listing
November 2018
1 Read

A level-headed approach to measuring direct oral anticoagulants: A 2-year retrospective analysis of DOAC levels from a tertiary UK centre.

Int J Lab Hematol 2018 Nov 8. Epub 2018 Nov 8.

Department of Haematology, Manchester Royal Infirmary, Manchester University Hospitals NHS Foundation Trust, Manchester, UK.

Introduction: Direct oral anticoagulants (DOAC) are commonly prescribed and measuring drug levels may be useful in a number of contexts. However, data on DOAC level measurement and their clinical utility in real-world studies are limited.

Methods: We carried out a 2-year retrospective cohort study of DOAC levels measured at our institution. Read More

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http://doi.wiley.com/10.1111/ijlh.12944
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http://dx.doi.org/10.1111/ijlh.12944DOI Listing
November 2018
6 Reads

ETP-ALL with aberrant B marker expression: Case series and a brief review of literature.

Int J Lab Hematol 2018 Nov 8. Epub 2018 Nov 8.

Department of Medical Oncology, Dr BRA IRCH, All India Institute of Medical Sciences (AIIMS), New Delhi, India.

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http://dx.doi.org/10.1111/ijlh.12942DOI Listing
November 2018
2 Reads
1.870 Impact Factor

Apoptosis: A biomarker of high-risk phenotype in pediatric acute myeloid leukemia?

Int J Lab Hematol 2018 Nov 1. Epub 2018 Nov 1.

Department of Medical Oncology, Dr. B. R. A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.

Introduction: Dysregulation of apoptosis has been explored in acute myeloid leukemia (AML); yet, its correlation with clinical outcomes in pediatric AML is unknown. This study was aimed to analyze percentage of apoptosis and apoptosis mediated through the intrinsic pathway with clinical outcomes in patients with pediatric AML.

Methods: This prospective study included pediatric AML patients enrolled from July 2013 to August 2016. Read More

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http://doi.wiley.com/10.1111/ijlh.12939
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http://dx.doi.org/10.1111/ijlh.12939DOI Listing
November 2018
8 Reads

Evaluating the performance of automated UV enzymatic assay for screening of glucose 6-phosphate dehydrogenase deficiency.

Int J Lab Hematol 2018 Nov 1. Epub 2018 Nov 1.

Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Introduction: A precise and reliable screening assay for glucose 6-phosphate dehydrogenase (G6PD) deficiency would greatly help avoiding unwanted outcomes due to bilirubin neurotoxicity in neonatal jaundice and antimalarial-induced haemolytic anaemia in malaria patients. Currently, available assays are laborious and require sophisticated laboratory expertise. This study aimed to evaluate the performance of a recently introduced automated screening assay for G6PD deficiency by comparing with a routine spectrophotometric assay. Read More

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http://dx.doi.org/10.1111/ijlh.12943DOI Listing
November 2018
7 Reads

Development of a multiplex assay to detect TERC and TERT mutations associated with immunosuppression therapy failure in Aplastic Anaemia patients.

Int J Lab Hematol 2018 Oct 27. Epub 2018 Oct 27.

Division of Haematology, Department of Pathology, University of Cape Town, Cape Town, South Africa.

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http://doi.wiley.com/10.1111/ijlh.12938
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http://dx.doi.org/10.1111/ijlh.12938DOI Listing
October 2018
3 Reads

A comparison of global coagulation assays between normal controls and patients with thrombocytopenia.

Int J Lab Hematol 2018 Oct 26. Epub 2018 Oct 26.

Department of Haematology, Northern Hospital, Epping, Victoria, Australia.

Introduction: Some patients with thrombocytopenia may be at risk of bleeding although quantitative platelet count is not always a sufficient predictive factor. Global coagulation assays such as thromboelastography (TEG ), calibrated automated thrombogram (CAT) and overall haemostatic potential (OHP) may provide a better assessment of an individual's haemostatic profile.

Methods: Blood samples were collected from thrombocytopenic patients. Read More

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http://doi.wiley.com/10.1111/ijlh.12941
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http://dx.doi.org/10.1111/ijlh.12941DOI Listing
October 2018
14 Reads

Comparison of light transmission aggregometry and multiple electrode aggregometry for the evaluation of patients with mucocutaneous bleeding.

Int J Lab Hematol 2018 Oct 25. Epub 2018 Oct 25.

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.

Introduction: The "gold standard" diagnostic test for assessing in vitro platelet function, light transmission aggregometry (LTA), has limitations to application because of sample requirements. Whole blood or multiple electrode aggregometry (MEA) using the Multiplate analyzer (Roche Diagnostics) requires smaller blood volumes and less sample manipulation than LTA, making it an attractive clinical testing option. Direct comparisons of MEA with LTA for diagnosis of platelet aggregation abnormalities are few. Read More

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http://doi.wiley.com/10.1111/ijlh.12937
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http://dx.doi.org/10.1111/ijlh.12937DOI Listing
October 2018
3 Reads

The diagnostic use of ADVIA 2120i Siemens and an "APL criteria" can help to reduce the rate of early death in the APL.

Int J Lab Hematol 2018 Oct 24. Epub 2018 Oct 24.

Department of Laboratory Medicine, Civitanova Marche, Italy.

Introduction: Acute promyelocytic leukemia (APL) is a type of acute myeloid leukemia (AML) with a life-threatening coagulopathy. Once it is suspected, ATRA should be started. Appreciation of APL details is critical, but an experienced hematopathologist may not be available. Read More

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http://doi.wiley.com/10.1111/ijlh.12935
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http://dx.doi.org/10.1111/ijlh.12935DOI Listing
October 2018
8 Reads

Significantly prolonged PT and APTT could indicate a wide spectrum of clinical manifestations in three patients with plasma cell disorders.

Int J Lab Hematol 2018 Oct 19. Epub 2018 Oct 19.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

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http://dx.doi.org/10.1111/ijlh.12936DOI Listing
October 2018
1 Read

The role of platelet hyperreactivity in venous thromboembolism after total knee arthroplasty in elderly population without pharmacologic prophylaxis: A single-center study.

Int J Lab Hematol 2018 Oct 17. Epub 2018 Oct 17.

Laboratory Medicine, Chungnam National University Hospital, Daejeon, South Korea.

Introduction: While it is suggested that platelet hyperreactivity plays a role in the arterial thrombi, its link with venous thromboembolism (VTE) is not well defined. Aggregometry using low concentrations of agonists is proposed as a reliable method to detect hyperreactivity. The aim of this study was to examine whether platelet hyperreactivity affects the development of VTE after total knee arthroplasty (TKA). Read More

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http://dx.doi.org/10.1111/ijlh.12932DOI Listing
October 2018
1 Read

The CD9 CD11b HLA-DR immunophenotype can be used to diagnose acute promyelocytic leukemia.

Int J Lab Hematol 2018 Oct 13. Epub 2018 Oct 13.

The Haematology Department, The Second Hospital of Shanxi Medical University, Taiyuan City, Shanxi Province, China.

Objective: To investigate the immunophenotypic characteristics of acute promyelocytic leukemia (APL) and explore the sensitivity and specificity of various antibody combinations for the timely and accurate diagnosis APL.

Methods: A retrospective analysis was performed using morphological, immunological, genetic, and molecular biological data from 92 patients diagnosed with APL and 190 controls diagnosed with non-APL acute myeloid leukemia.

Results: For APL diagnosis, the CD9/CD11b/human leukocyte antigen (HLA)-DR antibody combination had 85% sensitivity and 95% specificity, AUC = 0. Read More

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http://dx.doi.org/10.1111/ijlh.12929DOI Listing
October 2018
3 Reads

Are prothrombin time and clot waveform analysis useful in detecting a bleeding risk in liver cirrhosis?

Int J Lab Hematol 2018 Oct 9. Epub 2018 Oct 9.

Department of Medical Science and Public Health, University of Cagliari, Cagliari, Italy.

Introduction: Prothrombin time is thought to be unreliable in cirrhotic patients to predict the risk of bleeding. We investigated whether prothrombin time ratio was an independent risk factor for bleeding alongside its clot waveform analysis.

Methods: We studied 307 consecutive cirrhotic patients and 115 healthy subjects. Read More

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http://dx.doi.org/10.1111/ijlh.12934DOI Listing
October 2018
3 Reads

Hidden myelodysplastic syndrome (MDS): A prospective study to confirm or exclude MDS in patients with anemia of uncertain etiology.

Int J Lab Hematol 2018 Oct 5. Epub 2018 Oct 5.

Hematology, Hospital Universitario de Salamanca-IBSAL, Salamanca, Spain.

Introduction: Diagnosis of myelodysplastic syndromes (MDSs) when anemia is the only abnormality can be complicated. The aim of our study was to investigate the primary causes of anemia and/or macrocytosis of uncertain etiology.

Methods: We conducted a multicenter, prospective study over 4 months in three hematology laboratories. Read More

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http://doi.wiley.com/10.1111/ijlh.12933
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http://dx.doi.org/10.1111/ijlh.12933DOI Listing
October 2018
9 Reads

Atypical chronic lymphocytic leukemia: Brief historical overview and current usage of an equivocal concept.

Int J Lab Hematol 2018 Oct 5. Epub 2018 Oct 5.

Hematology Laboratory, ICO-Hospital Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.

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http://doi.wiley.com/10.1111/ijlh.12930
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http://dx.doi.org/10.1111/ijlh.12930DOI Listing
October 2018
2 Reads

Outcomes of 219 chronic myeloid leukaemia patients with additional chromosomal abnormalities and/or tyrosine kinase domain mutations.

Int J Lab Hematol 2018 Oct 4. Epub 2018 Oct 4.

The First Clinical Medical College, Lanzhou University, Lanzhou, Gansu, China.

Introduction: To confirm the role of additional chromosomal abnormalities (ACAs) and kinase domain (KD) mutations in the progression and outcomes of Chronic myeloid leukaemia (CML) patients and the connection between them, we analysed the ACAs and KD mutations of 219 CML patients admitted to our hospital.

Methods: Cytogenetic analysis of metaphases was performed to detect ACAs, and the BCR-ABL1 KD was sequenced to detect KD mutations.

Results: Twenty-four patients (11. Read More

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http://doi.wiley.com/10.1111/ijlh.12928
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http://dx.doi.org/10.1111/ijlh.12928DOI Listing
October 2018
4 Reads

The origin of sickle cell disease in Thailand.

Int J Lab Hematol 2018 Sep 27. Epub 2018 Sep 27.

Siriraj-Thalassaemia Centre, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

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http://dx.doi.org/10.1111/ijlh.12926DOI Listing
September 2018
10 Reads

The immunophenotypic characteristics and flow cytometric scoring system of acute myeloid leukemia with t(8;21) (q22;q22); RUNX1-RUNX1T1.

Int J Lab Hematol 2018 Sep 27. Epub 2018 Sep 27.

Department of Hematopathology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

Introduction: The translocation t(8;21) is one of the most frequent chromosome translocations in AML. Molecular (cyto)genetics is regarded as the gold standard for diagnosis. However, due to the complicated variety of AML-related genetic abnormalities, comprehensive screening for all of these abnormalities may not be cost-effective. Read More

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http://dx.doi.org/10.1111/ijlh.12916DOI Listing
September 2018
32 Reads

Paired APTTs of low and high lupus anticoagulant sensitivity permit distinction from other abnormalities and achieve good lupus anticoagulant detection rates in conjunction with dRVVT.

Int J Lab Hematol 2018 Sep 24. Epub 2018 Sep 24.

Department of Haemostasis and Thrombosis, Viapath Analytics, Guy's & St Thomas' Hospitals, London, UK.

Introduction: A prolonged activated partial thromboplastin time (APTT) may be indicative of a specific or multiple factor deficiency, therapeutic anticoagulation, presence of a nonspecific factor inhibitor, or lupus anticoagulant (LA). Recently, pairing of the LA-sensitive APTT and standard APTT reagents, Cephen LS and Cephen, respectively, has been shown to be effective in LA detection. The present study aimed to evaluate the usefulness of this reagent pair for discriminating between causes of APTT elevation and the detection of LA in conjunction with dilute Russell's viper venom time (dRVVT). Read More

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http://dx.doi.org/10.1111/ijlh.12921DOI Listing
September 2018
4 Reads

Use of a capillary specimen in the laboratory to verify a point-of-care international normalized ratio: Avoidance of a venipuncture in a pediatric setting.

Int J Lab Hematol 2018 Sep 14. Epub 2018 Sep 14.

Haematology Department, SA Pathology Women's and Children's Hospital, North Adelaide, South Australia, Australia.

Introduction: Point-of-care (POC) international normalized ratio (INR) values above an institutional cutoff are confirmed in the laboratory using a gold standard venous specimen. This can be problematic in a pediatric setting.

Method: In this study, 449 consecutive POC INR results were compared to an INR performed in the laboratory on a capillary citrate specimen collected from the same finger-stick. Read More

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http://doi.wiley.com/10.1111/ijlh.12920
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http://dx.doi.org/10.1111/ijlh.12920DOI Listing
September 2018
5 Reads

Carcinocythemia: A rare entity becoming more common? A 3-year, single institution series of seven cases and literature review.

Int J Lab Hematol 2018 Sep 14. Epub 2018 Sep 14.

Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin.

Introduction: Carcinocythemia is a rare phenomenon defined as morphologically identifiable, circulating tumor cells in the peripheral blood. No modern case series of carcinocythemia exists in the literature.

Methods: Blood smears from carcinocythemia patients were reviewed and associated clinicopathologic findings described and compared to the literature. Read More

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http://dx.doi.org/10.1111/ijlh.12924DOI Listing
September 2018
2 Reads

The association of HBG2, BCL11A, and HMIP polymorphisms with fetal hemoglobin and clinical phenotype in Iraqi Kurds with sickle cell disease.

Int J Lab Hematol 2018 Sep 14. Epub 2018 Sep 14.

ViennaLab Diagnostics GmbH, Wein, Austria.

Introduction: Fetal hemoglobin (HbF) is the major modifier for sickle cell disease (SCD) severity. HbF is modulated mainly by three major quantitative trait loci (QTL) on chromosomes 2, 6, and 11.

Methods: Five SNPs in the three QTLs (HBG2, rs7482144; BCL11A, rs1427407 and rs10189857; and HBS1L-MYB intergenic region, rs28384513 and rs9399137) were investigated by multiplex PCR and reverse hybridization, and their roles in HbF and clinical phenotype variability in Iraqi Kurds with SCD were assessed. Read More

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http://dx.doi.org/10.1111/ijlh.12927DOI Listing
September 2018
2 Reads

Biomarkers of disseminated intravascular coagulation in pediatric intensive care unit in Thailand.

Int J Lab Hematol 2018 Sep 12. Epub 2018 Sep 12.

Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Introduction: Disseminated intravascular coagulation (DIC) is a systemic activation of hemostatic system caused by several causes. Biomarkers including antithrombin (AT), protein C (PC), and thrombomodulin (TM) were reported as the additional markers for DIC in adults. This study aimed to determine the association between biomarkers among patients with overt DIC (ODIC) and nonovert DIC (NDIC) in children in PICU. Read More

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http://dx.doi.org/10.1111/ijlh.12917DOI Listing
September 2018
2 Reads

External quality assessment of p210 BCR-ABL1 transcript quantification by RT-qPCR: Findings and recommendations.

Int J Lab Hematol 2018 Sep 11. Epub 2018 Sep 11.

National Center for Clinical Laboratories, National Center of Gerontology, Beijing Hospital, Beijing, China.

Introduction: External quality assessment (EQA) is an essential tool for quality assurance of analytical testing processes of p210 BCR-ABL1 transcripts by RT-qPCR. As an EQA provider, the National Center for Clinical Laboratories organized an EQA scheme of p210 BCR-ABL1 testing in China for the first time to identify existing problems and ensure the reliability of p210 BCR-ABL1 testing.

Methods: Using armored RNA technology, we first constructed pACYC-MS2-p210 and CG recombinant plasmids and expressed p210 and CG armored RNAs, with packaging segments of p210 BCR-ABL1 fusion gene (FG) and four common control gene (CG) transcripts. Read More

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http://doi.wiley.com/10.1111/ijlh.12919
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http://dx.doi.org/10.1111/ijlh.12919DOI Listing
September 2018
4 Reads

Dilute Russell viper venom time interpretation and clinical correlation: A two-year retrospective institutional review.

Int J Lab Hematol 2018 Sep 8. Epub 2018 Sep 8.

Department of Laboratory Medicine, University of California, San Francisco, California.

Introduction: The dilute Russell viper venom time (dRVVT) detects lupus anticoagulant (LA). International Society for Thrombosis and Haemostasis (ISTH) guidelines specify positivity criteria, which differ from the assay manufacturer's criteria.

Methods: Two years of dRVVT testing at our institution were reviewed. Read More

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http://dx.doi.org/10.1111/ijlh.12925DOI Listing
September 2018
3 Reads

The importance of commutability in material used for quality control purposes.

Int J Lab Hematol 2018 Sep 5. Epub 2018 Sep 5.

UK NEQAS (Blood Coagulation), Sheffield, UK.

External Quality Assessment (EQA) is an important part of laboratory quality assurance. Spiking of normal plasma is sometimes employed to mimic clinical samples. It is important that spiked material gives similar results to clinical samples (ie, is commutable) to ensure appropriate conclusions can be drawn from EQA exercises. Read More

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http://doi.wiley.com/10.1111/ijlh.12918
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http://dx.doi.org/10.1111/ijlh.12918DOI Listing
September 2018
8 Reads

Screening of hereditary spherocytosis and pyruvate kinase deficiency by automated blood count using erythrocytic and reticulocytic parameters.

Int J Lab Hematol 2018 Dec 5;40(6):697-703. Epub 2018 Sep 5.

Department of Biological Hematology, Rouen University Hospital, Rouen, France.

Introduction: Development of additional parameters for complete blood count has emerged in recent hematology analyzers, leading to many publications. However, few studies have been conducted on advanced RBC parameters and hemolytic anemias. We investigated the interest of Sysmex unique parameters, MicroR and HypoHe, as well as the immature fraction of reticulocytes (IRF) in combination with complete blood and reticulocyte count, for screening hereditary spherocytosis (HS) and pyruvate kinase deficiency. Read More

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http://doi.wiley.com/10.1111/ijlh.12906
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http://dx.doi.org/10.1111/ijlh.12906DOI Listing
December 2018
10 Reads

Usability of femoral head bone marrow to verify reference ranges for the assessment of myelodysplasia by flow cytometry.

Int J Lab Hematol 2018 Dec 2;40(6):726-733. Epub 2018 Sep 2.

Department of Immunology, Laboratory Medical Immunology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, the Netherlands.

Background: A flow cytometry score (FCS) based on four parameters has been proposed for analysis of myelodysplastic syndromes patients with <5% blasts. We evaluated whether bone marrow aspirate samples isolated from femoral heads could be used for verification of cutoff values of the individual parameters score (IPS), contributing to the FCS and compared the applicability of FCS parameters in two centers.

Study Design And Methods: Bone marrow cells were obtained from femoral heads of patients who underwent hip replacement surgery. Read More

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http://dx.doi.org/10.1111/ijlh.12911DOI Listing
December 2018
5 Reads

GlycA is not a useful biomarker of inflammation in sickle cell disease.

Int J Lab Hematol 2018 Dec 27;40(6):704-709. Epub 2018 Aug 27.

Sickle Cell Branch, National Heart, Lung and Blood Institute, The National Institutes of Health, Bethesda, Maryland.

Introduction: Sickle cell disease (SCD) is a multisystemic disorder, the pathology being driven by recurrent inflammation particularly during a vaso-occlusive crisis. GlycA, a composite measure of protein glycation, is a sensitive biomarker for disorders associated with vascular inflammation. We determined the utility of GlycA as a biomarker of inflammation in SCD. Read More

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http://doi.wiley.com/10.1111/ijlh.12907
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http://dx.doi.org/10.1111/ijlh.12907DOI Listing
December 2018
16 Reads

Guidance for quality control practices and precision goals for CBCs based on IQMH patterns-of-practice survey.

Int J Lab Hematol 2018 Aug 23. Epub 2018 Aug 23.

Institute for Quality Management in Healthcare, Toronto, Ontario, Canada.

Introduction: Effective medical laboratory quality management systems ensure confidence in analyzing and reporting accurate and reliable patient results. To guarantee quality assurance, each laboratory needs appropriate internal quality control (IQC) procedures to monitor their test systems. The Institute for Quality Management in Healthcare (IQMH) Centre for Proficiency Testing conducted a survey on quality control (QC) practices in routine hematology. Read More

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http://dx.doi.org/10.1111/ijlh.12915DOI Listing
August 2018
1 Read

Biological variation estimates of prothrombin time, activated partial thromboplastin time, and fibrinogen in 28 healthy individuals.

Int J Lab Hematol 2018 Dec 20;40(6):721-725. Epub 2018 Aug 20.

Hematology, Ankara Numune Training and Research Hospital, Ankara, Turkey.

Background: Although tests of global hemostasis prothrombin time (PT) and activated partial thromboplastin time (aPTT) should not be used for prediction of bleeding risk, these tests are often used by many clinicians in daily practice particularly as a preoperative screening test. Robust biological variation (BV) data are needed for safe clinical applications of these tests. In this study, a stringent protocol was followed to estimate the BV's for PT, aPTT, and fibrinogen levels. Read More

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http://doi.wiley.com/10.1111/ijlh.12910
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http://dx.doi.org/10.1111/ijlh.12910DOI Listing
December 2018
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Evaluation of an immunochromatographic strip test for alpha-thalassaemia screening.

Int J Lab Hematol 2018 Dec 17;40(6):691-696. Epub 2018 Aug 17.

LabPlus, Haematology Department, Auckland City Hospital, Auckland, New Zealand.

Introduction: Hb H inclusion test (HbH-i) commonly used for α-thalassaemia screening is not standardised and is labour-intensive. This study evaluated a strip test based on immunochromatographic detection of Hb Bart's (ICT) for use as a routine screening test for α-thalassaemia screening in the clinical laboratory setting.

Methods: The performance characteristics of the ICT was determined by comparing the results of ICT and HbH-i on 67 patients, and the α-globin genotype on 47 of these patients who also had the molecular analysis. Read More

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http://doi.wiley.com/10.1111/ijlh.12905
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http://dx.doi.org/10.1111/ijlh.12905DOI Listing
December 2018
8 Reads

Marked acanthocytosis in the setting of Klippel-Trenaunay syndrome: A case report.

Int J Lab Hematol 2018 Aug 16. Epub 2018 Aug 16.

Laboratory Haematology Service, Department of Pathology, Centro Hospitalar Universitário do Porto, Porto, Portugal.

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http://dx.doi.org/10.1111/ijlh.12913DOI Listing
August 2018
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Easy discrimination of hematogones from lymphoblasts in B-cell progenitor acute lymphoblastic leukemia patients using CD81/CD58 expression ratio.

Int J Lab Hematol 2018 Dec 16;40(6):734-739. Epub 2018 Aug 16.

Laboratoire d'Hématologie, Réseau des Laboratoires Hospitaliers Universitaires de Bruxelles (LHUB-ULB), Bruxelles, Belgique.

Introduction: The discrimination of leukemia lymphoblasts (LB) in diagnosis and follow-up of B-cell progenitor acute lymphoblastic leukemia (BCP-ALL) by multiparameter flow cytometry (MFC) may be difficult due to the presence of hematogones (HG). The aim of this study was to compare lymphoblasts of BCP-ALL and HG for the expression of the most discriminating antigens.

Methods: A total of 82 bone marrow samples (39 BCP-ALL and 43 patients with HG) were analyzed using MFC. Read More

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http://dx.doi.org/10.1111/ijlh.12912DOI Listing
December 2018
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Live remote digital microscopy in peripheral blood smear evaluation: Intraobserver concordance and experience.

Int J Lab Hematol 2018 Dec 14;40(6):740-746. Epub 2018 Aug 14.

Department of Pathology and Laboratory Medicine, Indiana University school of Medicine, Indianapolis, Indiana.

Introduction: Peripheral blood smear (PBS) review is a routine laboratory test which requires pathologist's interpretation when abnormal indices, atypical cells, or critical findings are identified. Real-time remote digital microscopy (DM) can potentially facilitate rapid review when an on-site pathologist is not available. Herein, we assess intraobserver concordance of PBS evaluation with light microscopy (LM) and DM using VisionTek M6 robotic DM and TeamViewer imaging software. Read More

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http://dx.doi.org/10.1111/ijlh.12914DOI Listing
December 2018
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Results of a cross Canada survey of blood film review practice patterns by technologists and pathologists.

Int J Lab Hematol 2018 Dec 31;40(6):710-714. Epub 2018 Jul 31.

Kingston General Hospital, Kingston, Ontario, Canada.

Introduction: No common or widely accepted criteria exist for physician review of blood films or for reported standards of physician rate of blood film review. Individual institutions generally have internal criteria for physician review of blood films. To better understand how and why blood film reviews are performed at different institutions across Canada, with a specific interest in physician blood film review, we undertook a survey to assess the current practise patterns of physician review of blood films across Canada. Read More

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http://dx.doi.org/10.1111/ijlh.12908DOI Listing
December 2018
1 Read