1,521 results match your criteria International Journal of Laboratory Hematology [Journal]


Evaluation of a rapid and automated heparin-induced thrombocytopenia immunoassay.

Int J Lab Hematol 2019 Apr 15. Epub 2019 Apr 15.

Florida Hospital Center for Thrombosis Research, Winter Park, Florida.

Introduction: Heparin-induced thrombocytopenia (HIT) is a potentially life-threatening adverse reaction of heparin. Laboratory evaluation of HIT is often not available within a reasonable time. We evaluated the HemosIL HIT-Ab (Instrumentation Laboratory), a rapid, on-demand, fully automated, latex immunoturbidimetric assay (LIA). Read More

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http://dx.doi.org/10.1111/ijlh.13029DOI Listing
April 2019
1 Read

Impact of low volume citrate tubes on results of first-line hemostasis testing.

Int J Lab Hematol 2019 Apr 15. Epub 2019 Apr 15.

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

Introduction: Pediatric tubes are increasingly used for drawing blood for hemostasis testing. This study has investigated the potential impact of low volume citrate tubes on results of first-line hemostasis testing.

Methods: The study population comprised 34 patients on warfarin therapy and 17 ostensibly healthy volunteers. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/ijlh.13028
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http://dx.doi.org/10.1111/ijlh.13028DOI Listing
April 2019
1 Read

Importance of prognostic stratification via gene mutation analysis in elderly patients with acute myelogenous leukemia.

Int J Lab Hematol 2019 Apr 10. Epub 2019 Apr 10.

Department of Hematology, Nippon Medical School, Tokyo, Japan.

Introduction: Acute myelogenous leukemia (AML) in elderly patients is associated with an increased incidence of complications and treatment-related toxicity because of the frequency of comorbid disease and age-related deterioration in organ function. Despite advances in AML treatment in recent years, elderly patients have experienced limited benefit, and their outcomes remain poor. This study aimed to perform a comprehensive gene mutation analysis in elderly AML patients and identify gene mutations that could serve as prognostic factors. Read More

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http://dx.doi.org/10.1111/ijlh.13025DOI Listing
April 2019
4 Reads

Gene mutations associated with thrombosis detected by whole-exome sequencing in paroxysmal nocturnal hemoglobinuria.

Int J Lab Hematol 2019 Apr 10. Epub 2019 Apr 10.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Background: Thrombosis is a most common and lethal complication of paroxysmal nocturnal hemoglobinuria (PNH), which is a complex progression and its mechanism remains unclear. We tried to explore the possible genetic background of thrombosis in PNH patients and provide potential gene mutations associated with thrombosis in PNH patients.

Methods: The CD59 cells of 7 PNH and 6 PNH- aplastic anemia (AA) patients were sorted by flow cytometry and sequenced by whole-exome sequencing (WES). Read More

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http://dx.doi.org/10.1111/ijlh.13018DOI Listing
April 2019
1 Read

The potential value of thrombin generation assay in the diagnosis of FV inhibitors.

Int J Lab Hematol 2019 Apr 6. Epub 2019 Apr 6.

Laboratoire d'Hémostase, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Lyon, France.

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http://dx.doi.org/10.1111/ijlh.13027DOI Listing
April 2019
1 Read

A novel 223 kb deletion in the beta-globin gene cluster was identified in a Chinese thalassemia major patient.

Int J Lab Hematol 2019 Apr 4. Epub 2019 Apr 4.

Department of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Introduction: Although mutations in the human beta-globin gene cluster are essentially point mutations, several large deletions have been described in recent years.

Methods: We have identified a novel 223 kb deletion in a Chinese patient by multiplex ligation-dependent probe amplification and characterized it by next-generation sequencing, Gap-PCR, and DNA sequence analysis.

Results: The deletion extends from the 3'UTR of the δ globin gene (HBD) to 215 kb downstream of the HBB. Read More

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http://dx.doi.org/10.1111/ijlh.13021DOI Listing
April 2019
1 Read

Percentage of hypochromic red cells as a potential screening test to evaluate iron status in blood donors.

Int J Lab Hematol 2019 Apr 2. Epub 2019 Apr 2.

Hematology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.

Introduction: Haemoglobin (Hb) levels are used to assess eligibility for blood donation but are not correlated with iron status. The percentage of hypochromic red cells (%Hypo-He) has been suggested as a useful screening parameter for iron deficiency. The aim of this study was to determine the cut-off level and accuracy of %Hypo-He screening among blood donors. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/ijlh.13009
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http://dx.doi.org/10.1111/ijlh.13009DOI Listing
April 2019
5 Reads

Evaluation of an automated algorithm for interpretation of lupus anticoagulant testing.

Int J Lab Hematol 2019 Mar 12. Epub 2019 Mar 12.

Coagulation Laboratory, Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.

Introduction: Lupus anticoagulant (LAC) testing is a multistep procedure including screening, mixing, and confirmation tests. STA Coag Expert is a software module for STA R Max and STA Compact Max analyzers which includes an on-demand LAC algorithm, based on ISTH guidelines, for automatic interpretation, calculation, and launch of assays in LAC interpretation ("Stago coag algorithm").

Materials And Methods: One hundred ninety four patient samples were analyzed in parallel and interpreted manually and automatically by LAC algorithms. Read More

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http://dx.doi.org/10.1111/ijlh.13001DOI Listing
March 2019
1 Read

Interference in specialized coagulation assays affecting the protein C pathway: Effects of marked haemolysis, hyperbilirubinaemia and lipaemia on chromogenic and clotting tests on two coagulation platforms.

Int J Lab Hematol 2019 Mar 12. Epub 2019 Mar 12.

Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.

Background: Haemolysis, lipaemia and hyperbilirubinaemia represent important challenges in the coagulation laboratory. Test results are influenced not only by the degree of the interfering substance but also by the detection system.

Methods: We investigated the interference of free haemoglobin, triglycerides and bilirubin on a "modified activated protein C (APC) resistance test," protein C activity and protein S (antigen and activity) with two coagulation analysers, the STA-R Evolution and the ACL TOP. Read More

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http://dx.doi.org/10.1111/ijlh.13000DOI Listing

ROTEM reference ranges in a pregnant population from different nationalities/ethnic backgrounds.

Int J Lab Hematol 2019 Mar 4. Epub 2019 Mar 4.

Department of Clinical Chemistry, Haaglanden Medical Centre, The Hague, The Netherlands.

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http://dx.doi.org/10.1111/ijlh.12996DOI Listing
March 2019
2 Reads

Automated differential white blood cell count and cytological analysis can detect near-tetraploid cells in chronic lymphoproliferative disorders.

Int J Lab Hematol 2019 Mar 4. Epub 2019 Mar 4.

Service d'Hématologie Biologique, CHU Pitié-Salpêtrière, Assistance Publique Hôpitaux de Paris, Paris, France.

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http://dx.doi.org/10.1111/ijlh.12997DOI Listing
March 2019
1 Read

Evaluation of an immunochromatographic test for alpha thalassaemia screening in a multi-ethnic population.

Int J Lab Hematol 2019 Mar 4. Epub 2019 Mar 4.

College of Public Health, Medical & Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.

Introduction: The standard screening method for alpha thalassaemia is the examination of HbH preparation. It is labour intensive and poorly standardized. The development of a rapid strip immunochromatographic test (ICT) for haemoglobin Barts offers a fast, user friendly and cost-effective alternative screening tool. Read More

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http://dx.doi.org/10.1111/ijlh.12994DOI Listing
March 2019
1 Read
1.870 Impact Factor

In situ detection of JAK2V617F within viable hematopoietic cells using gold nanoparticle technology.

Int J Lab Hematol 2019 Mar 1. Epub 2019 Mar 1.

Istanbul Faculty of Medicine, Division of Hematology, Department of Internal Medicine, Istanbul University, Istanbul, Turkey.

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http://dx.doi.org/10.1111/ijlh.12991DOI Listing

Revisiting diagnostic criteria for myelodysplastic/myeloproliferative neoplasms with ring sideroblasts and thrombocytosis: Borderline cases without anemia exist.

Int J Lab Hematol 2019 Feb 27. Epub 2019 Feb 27.

Department of Pathology, Stanford University Medical Center, Stanford, California.

Introduction: Myelodysplastic/myeloproliferative neoplasm with ring sideroblasts and thrombocytosis (MDS/MPN-RS-T) is a rare disease in the 2016 revised World Health Organization (WHO) classification. Diagnostic criteria include the following: persistent thrombocytosis (>450 × 10 /L) with clustering of atypical megakaryocytes, refractory anemia, dyserythropoiesis with ring sideroblasts, and the presence of the spliceosome factor 3b subunit (SF3B1) mutation. It is unclear if anemia should be a required criterion for this diagnosis as cases which show all other features of MDS/MPN-RS-T but without anemia exist. Read More

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http://dx.doi.org/10.1111/ijlh.12981DOI Listing
February 2019
3 Reads

Immature granulocytes index as early marker of sepsis.

Int J Lab Hematol 2019 Feb 26. Epub 2019 Feb 26.

Pontifícia Universidade Católica of Rio Grande do Sul - PUCRS, Porto Alegre, Brazil.

Introduction: Sepsis induces the recruitment of immature neutrophils into the circulation. An immature granulocyte percentage (IG%) count greater than 3% has been shown to be an indicator for the risk of sepsis. The aim of this study was to evaluate the IG% as predictor of sepsis compared to blood culture results and sepsis diagnostic confirmation. Read More

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http://dx.doi.org/10.1111/ijlh.12990DOI Listing
February 2019
1 Read

The multifactorial pathogenesis of severe central anemia in a cohort of HIV-positive patients.

Int J Lab Hematol 2019 Feb 26. Epub 2019 Feb 26.

Department of Molecular Medicine and Haematology, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, South Africa.

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http://dx.doi.org/10.1111/ijlh.12986DOI Listing
February 2019
2 Reads

Unusual findings of acute myeloid leukemia with inv(3)(q21q26.2) or t(3;3)(q21;q26.2): A multicenter study.

Int J Lab Hematol 2019 Feb 22. Epub 2019 Feb 22.

Department of Hematology, School of Medicine, The Second Affiliated Hospital, Zhejiang University, Hangzhou, China.

Introduction: AML with inv(3)(q21.3q26.2) or t(3;3)(q21. Read More

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http://dx.doi.org/10.1111/ijlh.12987DOI Listing
February 2019
3 Reads

Age- and sex-specific reference intervals for hematologic analytes in Chinese children.

Int J Lab Hematol 2019 Feb 20. Epub 2019 Feb 20.

Department of Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Introduction: Reference intervals for pediatric laboratory tests need to be interpreted in the context of age- and sex-dependent dynamics. However, few reference intervals for healthy ethnic Han Chinese children have previously attempted to establish using large sample sizes. As such, there are no national hematological standards in China for pediatric reference intervals. Read More

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http://dx.doi.org/10.1111/ijlh.12979DOI Listing
February 2019
1 Read

Standardization of reticulocyte counts in the athlete biological passport.

Int J Lab Hematol 2019 Feb 19. Epub 2019 Feb 19.

World Anti-Doping Agency, Lausanne, Switzerland.

Introduction: The percentage of circulating reticulocytes (RET%) is a useful marker of blood doping in the context of the Athlete Biological Passport (ABP). The viability of the ABP depends on the comparability of sample data obtained across multiple laboratories for a given athlete. With the recent introduction of a different technology for the measurement of reticulocytes, the goal of this study was to compare currently employed Sysmex XT/XE analyzers to the recently introduced Sysmex XN analyzer. Read More

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http://dx.doi.org/10.1111/ijlh.12988DOI Listing
February 2019
4 Reads

Comparison of the bone marrow trephine sample quality between OnControl drill system and the Jamshidi needle.

Int J Lab Hematol 2019 Feb 19. Epub 2019 Feb 19.

Department of Haematology, Canberra Hospital, Canberra, Australian Capital Territory, Australia.

Introduction: Bone marrow biopsies are a key diagnostic and monitoring intervention in haematology with manual bone marrow techniques the established method of choice. Powered biopsy devices are now available, but are not widely used in haematology. This study compared the quality of bone marrow trephines obtained with the Jamshidi needle and OnControl powered drill system. Read More

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http://dx.doi.org/10.1111/ijlh.12984DOI Listing
February 2019

CD56 natural killer cells exhibit abnormal phenotype and function in severe aplastic anemia.

Int J Lab Hematol 2019 Feb 19. Epub 2019 Feb 19.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Introduction: CD56 NK cells have been highlighted to serve immunoregulatory functions. However, their roles in severe aplastic anemia (SAA) have not been elucidated.

Methods: Here, we investigated the quantities, phenotypes, cytokine secretion abilities, and the cytotoxicities of peripheral CD56 NK cells along with CD56 NK cells obtained from patients with SAA, SAA in remission (R-SAA), and healthy controls (HC). Read More

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http://dx.doi.org/10.1111/ijlh.12982DOI Listing
February 2019
1 Read

Lack of harmonization in high fluorescent cell automated counts with body fluids mode in ascitic, pleural, synovial, and cerebrospinal fluids.

Int J Lab Hematol 2019 Apr 13;41(2):277-286. Epub 2019 Feb 13.

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

Introduction: Cellular analysis in body fluids (BFs) provides important diagnostic information in various pathological settings. This study was hence aimed at comparing automated cell count obtained with Mindray BC-6800 (BC-BF) vs Sysmex XN-series (XN-BF) and evaluating other quantitative and qualitative information provided by these analyzers in ascitic (AF), pleural (PF), synovial (SF), and cerebrospinal (CSF) fluids.

Methods: Three hundred and fifty-one samples (99 AFs, 45 PFs, 75 SFs, and 132 CSFs) were analyzed in parallel with BC-BF, XN-BF, and optical microscopy (OM). Read More

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http://doi.wiley.com/10.1111/ijlh.12968
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http://dx.doi.org/10.1111/ijlh.12968DOI Listing
April 2019
3 Reads

Very unusual expression of multiple aberrant T-cell markers in plasmablastic plasma cell myeloma.

Int J Lab Hematol 2019 Feb 11. Epub 2019 Feb 11.

Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, Ohio.

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http://dx.doi.org/10.1111/ijlh.12985DOI Listing
February 2019
2 Reads

Evaluation of the detection of blasts by Sysmex hematology instruments, CellaVision DM96, and manual microscopy using flow cytometry as the confirmatory method.

Int J Lab Hematol 2019 Feb 11. Epub 2019 Feb 11.

Department of multidisciplinary laboratory medicine and medical biochemistry, Akershus University Hospital, Lørenskog, Norway.

Introduction: The aim of the present study was to evaluate the diagnostic ability of blast flags generated by Sysmex instruments (XE/XN) by comparing with immunophenotyping by flow cytometry (IFCM). Additionally, the ability of manual microscopy and CellaVision DM96 (pre- and reclassification) to predict the presence of "true" blasts was investigated.

Methods: Blood samples (n = 240) with suspect pathology flags reported by the XE were collected from the daily workload and examined by the XN, by manual microscopy, by CellaVision DM96 and by IFCM (CytoDiff Panel). Read More

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http://doi.wiley.com/10.1111/ijlh.12980
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http://dx.doi.org/10.1111/ijlh.12980DOI Listing
February 2019
30 Reads

Quantitative analysis of desmopressin (DDAVP) response in adult patients with type 1 von Willebrand disease.

Int J Lab Hematol 2019 Feb 8. Epub 2019 Feb 8.

Division of Hematology, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.

Introduction: Patients with Type 1 von Willebrand disease (VWD) have reduced amounts of von Willebrand factor (VWF) in their blood. Desmopressin (DDAVP) has been used to raise the blood levels of VWF in these patients. However, not all patients with Type 1 VWD are known to respond to DDAVP therapy. Read More

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http://dx.doi.org/10.1111/ijlh.12978DOI Listing
February 2019
1 Read

Flow cytometric predictive scoring systems for common fusions ETV6/RUNX1, BCR/ABL1, TCF3/PBX1 and rearrangements of the KMT2A gene, proposed for the initial cytogenetic approach in cases of B-acute lymphoblastic leukemia.

Int J Lab Hematol 2019 Feb 7. Epub 2019 Feb 7.

Flow Cytometry Laboratory, Department of Immunology, Athens Regional General Hospital "G. Gennimatas", Athens, Greece.

Introduction: In B-acute lymphoblastic leukemia (B-ALL), the identification of cytogenetic prognostic factors is important for stratifying patients into risk groups and tailoring treatment accordingly. The purpose of this study was to propose flow cytometric (FCM) scoring systems (SSs) for predicting t(12;21)(p13;q22), t(9;22)(q34;q11), t(11q23), and t(1;19)(q23;p13.3) translocations. Read More

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http://dx.doi.org/10.1111/ijlh.12983DOI Listing
February 2019
2 Reads

Optimal wavelength for the clot waveform analysis: Determination of the best resolution with minimal interference of the reagents.

Int J Lab Hematol 2019 Feb 7. Epub 2019 Feb 7.

Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Research Institute for Life Sciences (NARILIS), University of Namur, Namur, Belgium.

Introduction: Clot waveform analysis (CWA), a new methodology to assess coagulation process, can be usefully applied in various clinical settings. However, its clinical use is limited mainly because of the absence of standardization. No consensus exists regarding the wavelengths at which CWA has to be performed what is crucial for the sensitivity of the CWA. Read More

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http://doi.wiley.com/10.1111/ijlh.12975
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http://dx.doi.org/10.1111/ijlh.12975DOI Listing
February 2019
9 Reads

Very rare lineage switch from acute myeloid leukemia to mixed phenotype acute leukemia, B/Myeloid, during chemotherapy with no clonal evolution.

Int J Lab Hematol 2019 Feb 1. Epub 2019 Feb 1.

Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio.

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http://dx.doi.org/10.1111/ijlh.12977DOI Listing
February 2019
3 Reads

An in vitro study to investigate the interference of enoxaparin on plasma levels of direct oral factor Xa inhibitors measured by chromogenic assays.

Int J Lab Hematol 2019 Jan 30. Epub 2019 Jan 30.

Arianna Anticoagulazione Foundation, Bologna, Italy.

Introduction: Co-administration of enoxaparin and a direct oral factor Xa inhibitor (xabans: apixaban, edoxaban, rivaroxaban) could give rise to the problem of overlapping the anti-Xa activity when measuring direct oral anticoagulant (DOAC) levels. We aimed to evaluate in vitro the degree of the interference of increasing enoxaparin concentrations on xaban plasma levels measured by different chromogenic anti-Xa assays with drug-specific calibrators and controls.

Methods: Seven plasma samples were spiked with apixaban, edoxaban, or rivaroxaban at fixed concentration, and enoxaparin at increasing concentrations (0, 0. Read More

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http://dx.doi.org/10.1111/ijlh.12974DOI Listing
January 2019
1 Read

A pilot study to introduce a local external quality assurance scheme for D-dimers in the National Health Laboratory Service, in South Africa.

Int J Lab Hematol 2019 Apr 28;41(2):298-303. Epub 2019 Jan 28.

National Health Laboratory Service (NHLS), Department of Molecular Medicine and Haematology, University of the Witwatersrand (WITS), Johannesburg, South Africa.

Introduction: Laboratory quality assurance (QA) includes internal quality control (IQC), external quality assurance (EQA) and quality improvement (QI). EQA identifies quality deviations and training needs. D-dimers are breakdown products of thrombus and results guide various clinical decisions. Read More

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http://dx.doi.org/10.1111/ijlh.12973DOI Listing
April 2019
1 Read

Evaluation of the suitability of the World Health Organization International Reference Reagent for Hb A quantitation (89/666) for continued use.

Int J Lab Hematol 2019 Apr 20;41(2):262-270. Epub 2019 Jan 20.

Manchester Metropolitan University, Manchester, UK.

Introduction: The accurate determination of Hb A is a key marker when screening for a β-thalassaemia carrier. Data from external quality assessment (EQA) exercises have shown a lack of alignment of Hb A quantitation both within and between methods. The only reference material available for Hb A quantitative assay at the time of writing is the World Health Organization International Reference Reagent (89/666; WHO IRR) prepared in the 1980s and not validated for all current methodologies. Read More

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http://dx.doi.org/10.1111/ijlh.12966DOI Listing
April 2019
3 Reads

Platelet satellitism around lymphocytes: Case report and literature review.

Int J Lab Hematol 2019 Jan 20. Epub 2019 Jan 20.

Clinical Laboratory, Core-Hematology Department, Laboratori Clínic Metropolitana Nord (LCMN), Hospital Universitari Germans Trias i Pujol, Badalona, Spain.

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http://dx.doi.org/10.1111/ijlh.12972DOI Listing
January 2019
5 Reads

Utility of new red cell parameters for distinguishing functional iron deficiency from absolute iron deficiency in children with familial Mediterranean fever.

Int J Lab Hematol 2019 Apr 9;41(2):293-297. Epub 2019 Jan 9.

Department of Pediatric Rheumatology and Nephrology, Faculty of Medicine, Gazi University, Ankara, Turkey.

Introduction: Few data are available on the clinical utility of new red cell parameters for detecting anemia in children with inflammatory diseases. The aim was to investigate the utility of three new red cell parameters for distinguishing functional iron deficiency (FID) from absolute iron deficiency (AID) in children with familial Mediterranean fever (FMF).

Methods: The study involved 198 children with genetically confirmed FMF and 18 healthy-age and sex-matched controls. Read More

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http://doi.wiley.com/10.1111/ijlh.12971
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http://dx.doi.org/10.1111/ijlh.12971DOI Listing
April 2019
9 Reads

Acute myeloid leukemia with t(10;11)(p11-12;q23.3): Results of Russian Pediatric AML registration study.

Int J Lab Hematol 2019 Apr 9;41(2):287-292. Epub 2019 Jan 9.

Dmitry Rogachev National Medical and Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia.

Introduction: Translocations involving the KMT2A gene (also known as MLL) are frequently diagnosed in pediatric acute leukemia cases with either lymphoblastic or myeloid origin. KMT2A is translocated to multiple partner genes, including MLLT10/AF10 localizing at chromosomal band 10p12. KMT2A-MLLT10 is one of the common chimeric genes diagnosed in acute leukemia with KMT2A rearrangement (8%), especially in acute myeloid leukemia (AML; 18%). Read More

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http://dx.doi.org/10.1111/ijlh.12969DOI Listing
April 2019
18 Reads

Lnc-SOX6-1 upregulation correlates with poor risk stratification and worse treatment outcomes, and promotes cell proliferation while inhibits apoptosis in pediatric acute myeloid leukemia.

Int J Lab Hematol 2019 Apr 9;41(2):234-241. Epub 2019 Jan 9.

Department of Hematology and Oncology, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Introduction: To investigate the correlation of long noncoding RNA-SOX6-1 (lnc-SOX6-1) with clinicopathological features and treatment outcomes in pediatric acute myeloid leukemia (AML) patients, and further explore its function in AML cell proliferation and apoptosis.

Methods: A total of 146 de novo pediatric AML patients and 73 nonhematologic malignancy patients/donors were recruited. Bone marrow samples were obtained, followed by measurement of lnc-SOX6-1 expression by qPCR. Read More

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http://dx.doi.org/10.1111/ijlh.12952DOI Listing
April 2019
3 Reads

Development of new methodologies for the chromogenic estimation of betrixaban concentrations in plasma.

Int J Lab Hematol 2019 Apr 3;41(2):250-261. Epub 2019 Jan 3.

Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Research Institute for LIfe Sciences (NARILIS), University of Namur, Namur, Belgium.

Introduction: Chromogenic anti-Xa assays are the most appropriate tests to estimate the amount of betrixaban in plasma but the sensitivity of available tests is limited and improvements are needed to encompass the on-therapy range.

Methods: Betrixaban was spiked at concentrations ranging from 0 to 500 ng/mL in plasma from healthy donors. Three commercial tests were used (Biophen DiXaI , STA Liquid Anti-Xa, and HemosIL Liquid Anti-Xa), and adaptation of their sample dilution scheme was performed. Read More

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http://dx.doi.org/10.1111/ijlh.12963DOI Listing
April 2019
1 Read

A CBC algorithm combined with immature platelet fraction is able to identify JAK2 V617F mutation-positive polycythaemia vera patients.

Int J Lab Hematol 2019 Apr 2;41(2):271-276. Epub 2019 Jan 2.

Haematology Laboratory, Medlab Central, Palmerston North Hospital, Palmerston North, New Zealand.

Introduction: The recent lowering of the World Health Organisation (WHO) haemoglobin (Hb) and haematocrit (HCT) thresholds for diagnosis of polycythaemia vera (PV) has markedly increased the proportion of complete blood counts (CBC) that could be referred for haematologist review for comment, including advice on JAK2 V617F mutation analysis. The utility of an algorithm based on CBC indices and immature platelet fraction (IPF) to select cases for haematologist review was investigated.

Methods: IPF analysis was performed on 22 patients with known JAK2 V617F mutation and 41 patients who were negative for this mutation previously tested because of suspicion of PV. Read More

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http://dx.doi.org/10.1111/ijlh.12967DOI Listing
April 2019
8 Reads

Differentiation of homozygous hemoglobin E and hemoglobin E-β -thalassemia in children.

Int J Lab Hematol 2018 Dec 24. Epub 2018 Dec 24.

Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

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http://doi.wiley.com/10.1111/ijlh.12962
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http://dx.doi.org/10.1111/ijlh.12962DOI Listing
December 2018
4 Reads

Identification of a novel hemoglobin variant Hb Jilin [α139(HC1)Lys>Gln; HBA2:C.418 A>C] in a Chinese family.

Int J Lab Hematol 2018 Dec 22. Epub 2018 Dec 22.

Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China.

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http://doi.wiley.com/10.1111/ijlh.12964
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http://dx.doi.org/10.1111/ijlh.12964DOI Listing
December 2018
9 Reads
1.870 Impact Factor

Analytical performance of the new D-dimer and antithrombin assay on Roche cobas t 711 analyzer.

Int J Lab Hematol 2018 Dec 18. Epub 2018 Dec 18.

Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research, NSW Health Pathology, Westmead Hospital, Westmead, New South Wales, Australia.

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http://dx.doi.org/10.1111/ijlh.12957DOI Listing
December 2018
2 Reads

Peripheral blood lymphoagglutination and platelet satellitism in marginal zone lymphomas.

Int J Lab Hematol 2019 Apr 18;41(2):e50-e53. Epub 2018 Dec 18.

Hospices Civils de Lyon, Lyon, Rhône-Alpes, France.

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http://dx.doi.org/10.1111/ijlh.12956DOI Listing
April 2019
1 Read

Clotting and chromogenic factor VIII assay variability in post-infusion and spiked samples containing full-length recombinant FVIII or recombinant factor VIII Fc fusion protein (rFVIIIFc).

Int J Lab Hematol 2019 Apr 17;41(2):176-183. Epub 2018 Dec 17.

UK NEQAS Blood Coagulation, Sheffield, UK.

Introduction: Variability in FVIII measurement is a recognized problem. There are limited data for samples containing recombinant Factor VIII Fc fusion protein (rFVIIIFc). Many studies use samples for which factor concentrate has been spiked into FVIII deficient plasma in vitro. Read More

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http://dx.doi.org/10.1111/ijlh.12940DOI Listing
April 2019
3 Reads

Eosin-5'-maleimide binding test-Do we use appropriate reference values to detect hereditary spherocytosis in neonates?

Int J Lab Hematol 2018 Dec 10. Epub 2018 Dec 10.

Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland.

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December 2018
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Evaluation of next-generation sequencing-based clonality analysis of T-cell receptor gamma gene rearrangements based on a new interpretation algorithm.

Int J Lab Hematol 2019 Apr 8;41(2):242-249. Epub 2018 Dec 8.

Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium.

Introduction: T-cell receptor gene (TRG) rearrangement profiling is an essential component of the workup at diagnosis of T-cell malignancies. TRG amplification by polymerase chain reaction (PCR) and analysis by capillary electrophoresis (PCR-CE) is mostly widely used but is hampered by a subjective interpretation of its results and possible false-positive interpretation of clonality. Several studies evaluated the advantage of TRG rearrangement analysis by Next Generation Sequencing (TRG-NGS), however few have proposed an adequate data interpretation algorithm. Read More

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http://dx.doi.org/10.1111/ijlh.12954DOI Listing
April 2019
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