185,316 results match your criteria Insight: The Journal of the American Society of Ophthalmic Registered Nurses [Journal]


Multiplatform metabolomics provides insight into the molecular basis of chronic kidney disease.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 3;1117:49-57. Epub 2019 Apr 3.

Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

Changes in metabolites composition can reflect currently present pathological processes in a living organism and constitute a basis for diagnosis and treatment improvements. Thus, the multiplatform metabolomics approach was applied for the investigation of molecular mechanisms of chronic kidney disease (CKD) progression. The high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS) and gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ/MS) serum metabolic fingerprinting followed by uni- and multivariate statistical analysis was carried out to determine metabolic pattern differentiating CKD patients and healthy controls. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.003DOI Listing

Trophic transfer of cyclic methyl siloxanes in the marine food web in the Bohai Sea, China.

Ecotoxicol Environ Saf 2019 Apr 15;178:86-93. Epub 2019 Apr 15.

International Joint Research Centre for Persistent Toxic Substances (IJRC-PTS), College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China. Electronic address:

Trophic transfer of cyclic methyl siloxanes (CMS) in aquatic ecosystems is an important criterion for assessing its environmental risks. This study researched the trophic transfer of four CMS (octamethylcyclotetrasiloxane (D), decamethylcyclopentasiloxane (D), dodecamethylcyclohexasiloxane (D), and tetradecamethylcycloheptasiloxane (D)) in marine food web from zooplankton up to seabirds in the Chinese Bohai Sea. In the zooplankton-invertebrate-fish-seabird based food web, the significant trophic magnifications were found for D to D (D: R = 0. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.034DOI Listing

Autophagy represses fascaplysin-induced apoptosis and angiogenesis inhibition via ROS and p8 in vascular endothelia cells.

Biomed Pharmacother 2019 Apr 15;114:108866. Epub 2019 Apr 15.

School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.

Fascaplysin is a natural product isolated from marine sponges that exhibits broad anticancer activity. Previous studies revealed that fascaplysin-induced apoptosis and angiogenesis inhibition in vascular endothelial cells contributed to its anticancer activity. Accumulating evidence indicates that autophagy plays a significant role in mediating the function of vascular endothelial cells (VECs) and the response to cancer therapy. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108866DOI Listing

The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis.

Mol Plant 2019 Apr 15. Epub 2019 Apr 15.

Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai, China; John Innes Centre, Norwich, NR4 7UH UK. Electronic address:

Scutellaria baicalensis Georgi is important in Chinese Traditional Medicine where preparations of dried roots, 'Huang Qin', are used for liver and lung complaints including complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S16742052193013
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http://dx.doi.org/10.1016/j.molp.2019.04.002DOI Listing
April 2019
7 Reads

Leishmania infantum LeIF and its recombinant polypeptides induce the maturation of dendritic cells in vitro: an insight for dendritic cells based vaccine.

Immunol Lett 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Molecular Epidemiology and Experimental Pathology, Institut Pasteur de Tunis, Université Tunis El Manar, 13 Place Pasteur, BP 74, 1002 Tunis-Belvedère, Tunisia. Electronic address:

We previously showed that recombinant Leishmania infantum eukaryotic initiation factor (LieIF) was able to induce the secretion of cytokines IL-12, IL-10 and TNF-α by human monocytes. In this study, we explored in vitro the potential of LieIF to induce phenotypic maturation and functional differentiation of murine bone - marrow derived dendritic cells (BM-DCs). Moreover, in order to identify potential immunnomodulatory regions of LieIF, eight recombinant overlapping protein fragments covering the whole amino acid sequence ofprotein; were constructed and assessed in vitro for their ability to induce maturation of BM-DCs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01652478183041
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http://dx.doi.org/10.1016/j.imlet.2019.04.001DOI Listing
April 2019
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Unveiling toxicological aspects of venom from the Aesculapian False Coral Snake Erythrolamprus aesculapii.

Toxicon 2019 Apr 15. Epub 2019 Apr 15.

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto Nacional de Medicina Tropical (INMeT), Neuquén y Jujuy s/n, 3370, Puerto Iguazú, Argentina. Electronic address:

Most colubrid snake venoms have been poorly studied, despite the fact that they represent a great resource for biological, ecological, toxinological and pharmacological research. Herein, we explore the venom delivery system of the Aesculapian False Coral Snake Erythrolamprus aesculapii as well as some biochemical and toxicological properties of its venom. Its Duvernoy's venom gland is composed of serous secretory cells arranged in densely packed secretory tubules, and the most striking feature of its fang is their double-curved shape, exhibiting a beveled bladelike appearance near the tips. Read More

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http://dx.doi.org/10.1016/j.toxicon.2019.04.007DOI Listing

Mesenchymal Stem Cells: from Regeneration to Cancer.

Pharmacol Ther 2019 Apr 15. Epub 2019 Apr 15.

The Jackson Laboratory, Bar Harbor, ME 04609, USA. Electronic address:

Mesenchymal stem cells (MSCs) are multipotent tissue stem cells that differentiate into a number of mesodermal tissue types, including osteoblasts, adipocytes, chondrocytes and myofibroblasts. MSCs were originally identified in the bone marrow (BM) of humans and other mammals, but recent studies have shown that they are multilineage progenitors in various adult organs and tissues. MSCs that localize at perivascular sites function to rapidly respond to external stimuli and coordinate with the vascular and immune systems to accomplish the wound healing process. Read More

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http://dx.doi.org/10.1016/j.pharmthera.2019.04.005DOI Listing

Chronic Kidney Disease in Agricultural Communities.

Am J Med 2019 Apr 15. Epub 2019 Apr 15.

Department of Biomedical Informatics, The Ohio State University. Electronic address:

Patients residing in agricultural communities have a high risk of developing chronic kidney disease. In the Great Plains, geo-environmental risk factors (variable climate, temperature, air quality, water quality and drought) combine with agro-environmental risk factors (exposure to fertilizers, soil conditioners, herbicides, fungicides and pesticides) to increase risk for toxic nephropathy. However, research defining the specific influence of agricultural chemicals on the progression of kidney disease in rural communities has been somewhat limited. Read More

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http://dx.doi.org/10.1016/j.amjmed.2019.03.036DOI Listing

Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.

Arch Biochem Biophys 2019 Apr 15. Epub 2019 Apr 15.

Nanoscience & Nanotechnology & Innovative Instrumentation (NAST) Centre, University of Rome Tor Vergata, Rome, Italy; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, USA. Electronic address:

Matrix vesicles (MVs) are a class of extracellular vesicles that initiate mineralization in cartilage, bone, and other vertebrate tissues by accumulating calcium ions (Ca) and inorganic phosphate (P) within their lumen and forming a nucleation core (NC). After further sequestration of Ca and P, the NC transforms into crystalline complexes. Direct evidence of the existence of the NC and its maturation have been provided solely by analyses of dried samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183104
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http://dx.doi.org/10.1016/j.abb.2019.04.003DOI Listing
April 2019
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KLF4 activates NFκB signaling and esophageal epithelial inflammation via the Rho-related GTP-binding protein RHOF.

PLoS One 2019 18;14(4):e0215746. Epub 2019 Apr 18.

Division of Gastroenterology, University of Pennsylvania Perelman School of Medicine, Philadelphia, United States of America.

Understanding the regulatory mechanisms within esophageal epithelia is essential to gain insight into the pathogenesis of esophageal diseases, which are among the leading causes of morbidity and mortality throughout the world. The zinc-finger transcription factor Krüppel-like factor (KLF4) is implicated in a large number of cellular processes, such as proliferation, differentiation, and inflammation in esophageal epithelia. In murine esophageal epithelia, Klf4 overexpression causes chronic inflammation which is mediated by activation of NFκB signaling downstream of KLF4, and this esophageal inflammation produces epithelial hyperplasia and subsequent esophageal squamous cell cancer. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215746PLOS

RNA sequencing of intestinal mucosa reveals novel pathways functionally linked to celiac disease pathogenesis.

PLoS One 2019 18;14(4):e0215132. Epub 2019 Apr 18.

Mass General Hospital for Children and Division of Pediatric Gastroenterology and Nutrition, Harvard Medical School, Boston, Massachusetts, United States of America.

Background & Aims: The early steps in the pathophysiology of celiac disease (CD) leading to loss of tolerance to gluten are poorly described. Our aim was to use RNA sequencing of duodenal biopsies in patients with active CD, CD in remission, and non-CD controls to gain insight into CD pathophysiology, identify additional genetic signatures linked to CD, and possibly uncover targets for future therapeutic agents.

Methods: We performed whole transcriptome shotgun sequencing of intestinal biopsies in subjects with active and remission CD and non-CD controls. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215132PLOS

Varicose veins of lower extremities: Insights from the first large-scale genetic study.

PLoS Genet 2019 Apr 18;15(4):e1008110. Epub 2019 Apr 18.

Laboratory of Theoretical and Applied Functional Genomics, Novosibirsk State University, Novosibirsk, Russia.

Varicose veins of lower extremities (VVs) are a common multifactorial vascular disease. Genetic factors underlying VVs development remain largely unknown. Here we report the first large-scale study of VVs performed on a freely available genetic data of 408,455 European-ancestry individuals. Read More

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http://dx.doi.org/10.1371/journal.pgen.1008110DOI Listing

The Pattern of Acetabular Cartilage Wear Is Hip Morphology-dependent and Patient Demographic-dependent.

Clin Orthop Relat Res 2019 May;477(5):1021-1033

C. Pascual-Garrido, E. L. Yanik, J. C. Clohisy, Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, MO, USA D. J. Li, University of Miami Miller School of Medicine, Miami, FL, USA G. Grammatopoulos, Nuffield Orthopaedic Care, University of Oxford, Oxford, UK ANCHOR Group, Washington University, St Louis, MO 63110, USA.

Background: Acetabular cartilage damage has been described in patients with femoroacetabular impingement (FAI). However, most reports of articular cartilage damage in hip FAI have been focused on the acetabular cartilage and derived from single-center, retrospective studies of relatively small patient cohorts. Identifying patterns of articular cartilage wear is important in patient selection, treatment prognosis, and determining whether patterns of intraarticular cartilage wear are secondary to abnormal hip morphology. Read More

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http://dx.doi.org/10.1097/CORR.0000000000000649DOI Listing

Phase 1 trial of intrapleural LTI-01; single chain urokinase in complicated parapneumonic effusions or empyema.

JCI Insight 2019 Apr 18;5. Epub 2019 Apr 18.

Background: Current dosing of intrapleural fibrinolytic therapy (IPFT) in adults with complicated parapneumonic effusion (CPE) / empyema is empiric, as dose-escalation trials have not previously been conducted. We hypothesized that LTI-01 (scuPA), which is relatively resistant to PA inhibitor-1 (PAI-1), would be well-tolerated.

Methods: This was an open-label, dose-escalation trial of LTI-01 IPFT at 50,000-800,000 IU daily for up to 3 days in adults with loculated CPE/empyema and failed pleural drainage. Read More

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http://dx.doi.org/10.1172/jci.insight.127470DOI Listing

Prohibitin promotes de-differentiation and is a potential therapeutic target in neuroblastoma.

JCI Insight 2019 Apr 18;5. Epub 2019 Apr 18.

Gain of the long arm of chromosome 17 (17q) is a cytogenetic hallmark of high-risk neuroblastoma, yet its contribution to neuroblastoma pathogenesis remains incompletely understood. Combining whole-genome and RNA sequencing of neuroblastomas, we identified the prohibitin (PHB) gene as highly expressed in tumors with 17q gain. High PHB expression correlated with poor prognosis and was associated with loss of gene expression programs promoting neuronal development and differentiation. Read More

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http://dx.doi.org/10.1172/jci.insight.127130DOI Listing

Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B.

JCI Insight 2019 Apr 18;5. Epub 2019 Apr 18.

The bone marrow microenvironment (BMME) contributes to the regulation of hematopoietic stem cell (HSC) function, though its role in age-associated lineage skewing is poorly understood. Here we show that dysfunction of aged marrow macrophages (Mφs) directs HSC platelet-bias. Mφs from the marrow of aged mice and humans exhibited an activated phenotype, with increased expression of inflammatory signals. Read More

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http://dx.doi.org/10.1172/jci.insight.124213DOI Listing

Blocking IL-10 receptor signaling ameliorates Mycobacterium tuberculosis infection during influenza-induced exacerbation.

JCI Insight 2019 Apr 18;5. Epub 2019 Apr 18.

Epidemiological findings indicate that coinfection with influenza viruses is associated with an increased risk of death in patients suffering from tuberculosis but the underlying pathomechanisms are not well understood. In this study, we demonstrate that influenza A virus (IAV) coinfection rapidly impairs control of Mycobacterium tuberculosis (Mtb) in C57BL/6 mice. IAV coinfection was associated with significantly increased bacterial loads, reduced survival and a substantial modulation of innate and adaptive immune defenses including an impaired onset and development of Mtb-specific CD4+ T cell responses and the accumulation of macrophages with increased arginase-1 production in the lungs. Read More

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http://insight.jci.org/articles/view/126533
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http://dx.doi.org/10.1172/jci.insight.126533DOI Listing
April 2019
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Down syndrome mouse models have an abnormal enteric nervous system.

JCI Insight 2019 Apr 18;5. Epub 2019 Apr 18.

Children with trisomy 21 (Down syndrome [DS]) have a 130-fold increased incidence of Hirschsprung Disease (HSCR), a developmental defect where the enteric nervous system (ENS) is missing from distal bowel (i.e., distal bowel is aganglionic). Read More

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http://dx.doi.org/10.1172/jci.insight.124510DOI Listing

Glutamine side chain 13C=18O as a non-perturbative IR probe of amyloid fibril hydration and assembly.

J Am Chem Soc 2019 Apr 17. Epub 2019 Apr 17.

Infrared (IR) spectroscopy has provided considerable insights into the structures, dynamics, and formation mechanisms of amyloid fibrils. IR probes, such as main chain 13C=18O, have been widely employed to obtain site-specific structural information, yet only secondary structures and strand-to-strand arrangements can be probed. Very few non-perturbative IR probes are available to report on side chain conformation and environments, which are critical to determining sheet-to-sheet arrangements in steric zippers within amyloids. Read More

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http://dx.doi.org/10.1021/jacs.9b00577DOI Listing

Ru-Based Catechothiolate Complexes Bearing an Unsaturated NHC Ligand: Effective Cross-Metathesis Catalysts for Synthesis of ( Z)-α,β-Unsaturated Esters, Carboxylic Acids, and Primary, Secondary, and Weinreb Amides.

J Am Chem Soc 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, Merkert Chemistry Center , Boston College , Chestnut Hill , Massachusetts 02467 , United States.

Despite notable progress, olefin metathesis methods for preparation of ( Z)-α,β-unsaturated carbonyl compounds, applicable to the synthesis of a large variety of bioactive molecules, remain scarce. Especially desirable are transformations that can be promoted by ruthenium-based catalysts, as such entities would allow direct access to carboxylic esters and amides, or acids (in contrast to molybdenum- or tungsten-based alkylidenes). Here, we detail how, based on the mechanistic insight obtained through computational and experimental studies, a readily accessible ruthenium catechothiolate complex was found that may be used to generate many α,β-unsaturated carbonyl compounds in up to 81% yield and ≥98:2 Z/ E ratio. Read More

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http://dx.doi.org/10.1021/jacs.9b02318DOI Listing

Better Guidance for Surrogates.

Hastings Cent Rep 2019 Mar;49(2)

The March-April issue of the Hastings Center Report offers another in a series of articles over the last few years on the structure and the ethics of surrogate decision-making. Here, Daniel Brudney addresses how to help the surrogate deal with a treatment decision. A core insight he offers is that the structure of the surrogate's decision has been misunderstood and the misunderstanding makes the task yet harder. Read More

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http://dx.doi.org/10.1002/hast.984DOI Listing

A numbering-up strategy of hydrodynamic microfluidic filters for continuous-flow high-throughput cell sorting.

Lab Chip 2019 Apr 18. Epub 2019 Apr 18.

Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

Even though a number of microfluidic systems for particle/cell sorting have been proposed, facile and versatile platforms that provide sufficient sorting throughput and good operability are still under development. Here we present a simple but effective numbering-up strategy to dramatically increase the throughput of a continuous-flow particle/cell sorting scheme based on hydrodynamic filtration (HDF). A microfluidic channel equipped with multiple branches has been employed as a unit structure for size-based filtration, which realizes precise sorting without necessitating sheath flows. Read More

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http://dx.doi.org/10.1039/c9lc00053dDOI Listing

Wearable Sensors Reveal Menses-Driven Changes in Physiology and Enable Prediction of the Fertile Window: Observational Study.

J Med Internet Res 2019 Apr 18;21(4):e13404. Epub 2019 Apr 18.

Department of Reproductive Endocrinology, University Hospital, Zurich, Switzerland.

Background: Previous research examining physiological changes across the menstrual cycle has considered biological responses to shifting hormones in isolation. Clinical studies, for example, have shown that women's nightly basal body temperature increases from 0.28 to 0. Read More

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http://www.jmir.org/2019/4/e13404/
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http://dx.doi.org/10.2196/13404DOI Listing
April 2019
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A unified framework of longitudinal models to examine reciprocal relations.

Psychol Methods 2019 Apr 18. Epub 2019 Apr 18.

Department of Methodology and Statistics, Utrecht University.

Inferring reciprocal effects or causality between variables is a central aim of behavioral and psychological research. To address reciprocal effects, a variety of longitudinal models that include cross-lagged relations have been proposed in different contexts and disciplines. However, the relations between these cross-lagged models have not been systematically discussed in the literature. Read More

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http://doi.apa.org/getdoi.cfm?doi=10.1037/met0000210
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http://dx.doi.org/10.1037/met0000210DOI Listing
April 2019
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Vapor Phase Growth of Semiconductor Nanowires: Key Developments and Open Questions.

Chem Rev 2019 Apr 18. Epub 2019 Apr 18.

Laboratory of Semiconductor Materials, Institute of Materials , École Polytechnique Fédérale de Lausanne , 1015 Lausanne , Switzerland.

Nanowires are filamentary crystals with a tailored diameter that can be obtained using a plethora of different synthesis techniques. In this review, we focus on the vapor phase, highlighting the most influential achievements along with a historical perspective. Starting with the discovery of VLS, we feature the variety of structures and materials that can be synthesized in the nanowire form. Read More

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http://dx.doi.org/10.1021/acs.chemrev.8b00649DOI Listing

Visual P300 Mind-Speller Brain-Computer Interfaces: A Walk Through the Recent Developments With Special Focus on Classification Algorithms.

Clin EEG Neurosci 2019 Apr 18:1550059419842753. Epub 2019 Apr 18.

1 Department of Electronics and Communication Engineering, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India.

Brain-computer interfaces are sophisticated signal processing systems, which directly operate on neuronal signals to identify specific human intents. These systems can be applied to overcome certain disabilities or to enhance the natural capabilities of human beings. The visual P300 mind-speller is a prominent one among them, which has opened up tremendous possibilities in movement and communication applications. Read More

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http://dx.doi.org/10.1177/1550059419842753DOI Listing

Role of cytokines in combinatorial immunotherapeutics of non-small cell lung cancer through systems perspective.

Cancer Med 2019 Apr 17. Epub 2019 Apr 17.

National Centre for Cell Science, SP Pune University Campus, Pune, India.

Lung cancer is the leading cause of deaths related to cancer and accounts for more than a million deaths per year. Various new strategies have been developed and adapted for treatment; still the survival for 5 years is just 16% in patients with non-small cell lung cancer (NSCLC). Most of these strategies to combat NSCLC whether it is a drug molecule or immunotherapy/vaccine candidate require a big cost and time. Read More

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http://dx.doi.org/10.1002/cam4.2112DOI Listing

RNA dynamics by NMR.

Chembiochem 2019 Apr 17. Epub 2019 Apr 17.

Karolinska Institute, Medical Biochemistry and Biophysics, Scheeles Väg 2, 17177, Stockholm, SWEDEN.

An ever-increasing number of functional RNAs require mechanistic understanding. RNA function relies on changes in its structure, so-called dynamics. To reveal dynamic processes and higher energy structures, new Nuclear Magnetic Resonance (NMR) methods have been developed to elucidate these dynamics in RNA with atomic resolution. Read More

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http://dx.doi.org/10.1002/cbic.201900072DOI Listing

New insight into poly (3-hydroxybutyrate) production by Azomonas macrocytogenes isolate KC685000: large scale production, kinetic modeling, recovery and characterization.

Mol Biol Rep 2019 Apr 17. Epub 2019 Apr 17.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Abbassia, 11566, Cairo, Egypt.

About 24 h incubation of Azomonas (A.) macrocytogenes isolate KC685000 in 14L fermenter produced 22% poly (3-hydroxybutyrate) (PHB) per cell dry weight (CDW) biopolymer using 1 vvm aeration, 10% inoculum size, and initial pH of 7.2. Read More

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http://dx.doi.org/10.1007/s11033-019-04798-4DOI Listing

NAV2 facilitates invasion of cutaneous melanoma cells by targeting SNAI2 through the GSK-3β/β-catenin pathway.

Arch Dermatol Res 2019 Apr 17. Epub 2019 Apr 17.

Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.

Previous studies have identified neuron navigator 2(NAV2) as an oncogene in several human tumors. However, the NAV2 gene expression changes and its role in the pathogenesis of cutaneous melanoma have not been clearly illustrated. Further investigations of NAV2 in cutaneous melanoma may provide new mechanistic insight and treatment strategy for this disease. Read More

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http://dx.doi.org/10.1007/s00403-019-01909-wDOI Listing

The impact of insight on post-lobectomy complications.

J Thorac Dis 2019 Mar;11(Suppl 3):S289-S290

Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas, USA.

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http://dx.doi.org/10.21037/jtd.2019.01.38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424774PMC

'' Current childcare practices and caregivers' perceptions of and receptivity to early childhood development activities in rural Burkina Faso.

BMJ Glob Health 2019 30;4(2):e001233. Epub 2019 Mar 30.

Department of Epidemiology and Public Health, Institute for Global Health, University College London, London, UK.

Introduction: Effective stimulation and responsive caregiving during the first 2 years is crucial for children's development. By age 3-4 years, over 40% of children in sub-Saharan Africa fail to meet basic cognitive or socioemotional milestones, but there are limited data on parenting and childcare practices. This study, conducted to inform the design of a mass media intervention, explored practices, perceptions, motivators and obstacles to childhood development-related practices among parents and caregivers of children aged 0-2 years in rural Burkina Faso. Read More

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http://dx.doi.org/10.1136/bmjgh-2018-001233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441292PMC

gene predicts clinical recovery after sustained virological response in decompensated hepatitis C cirrhosis.

BMJ Open Gastroenterol 2019 12;6(1):e000241. Epub 2019 Mar 12.

Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Missouri, USA.

Background: Patients with decompensated hepatitis C virus (HCV) cirrhosis experience various outcomes after sustained virological response (SVR), ranging from clinical recovery to further deterioration. We hypothesised that the genetic risk for steatosis, namely the polymorphisms rs738409 of Patatin-like Phospholipase Domain-Containing 3 (), rs58542926 of Transmembrane-6-Superfamily-2 (), and rs641738 of Membrane-bound O-acyltransferase Domain-Containing 7 (), is predictive of recovery.

Methods: We prospectively enrolled 56 patients with Child-Pugh (CPT) B/C cirrhosis who underwent antiviral therapy. Read More

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http://dx.doi.org/10.1136/bmjgast-2018-000241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441264PMC

Analysis of the Attune tibial tray backside: A comparative retrieval study.

Bone Joint Res 2019 Mar 2;8(3):136-145. Epub 2019 Apr 2.

Institute of Orthopaedics and Musculoskeletal Science, University College London and the Royal National Orthopaedic Hospital, Stanmore, UK.

Objectives: The Attune total knee arthroplasty (TKA) has been used in over 600 000 patients worldwide. Registry data show good clinical outcome; however, concerns over the cement-tibial interface have been reported. We used retrieval analysis to give further insight into this controversial topic. Read More

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http://dx.doi.org/10.1302/2046-3758.83.BJJ-2018-0102.R2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446526PMC
March 2019
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Density artefacts at interfaces caused by multiple time-step effects in molecular dynamics simulations.

F1000Res 2018 5;7:1745. Epub 2018 Nov 5.

Laboratory of Physical Chemistry, ETH Zürich, Zurich, 8093, Switzerland.

Molecular dynamics (MD) simulations have become an important tool to provide insight into molecular processes involving biomolecules such as proteins, DNA, carbohydrates and membranes. As these processes cover a wide range of time scales, multiple time-step integration methods are often employed to increase the speed of MD simulations. For example, in the twin-range (TR) scheme, the nonbonded forces within the long-range cutoff are split into a short-range contribution updated every time step (inner time step) and a less frequently updated mid-range contribution (outer time step). Read More

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http://dx.doi.org/10.12688/f1000research.16715.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441880PMC
November 2018

A theoretical insight into the reaction mechanisms of a 2,4,6-trinitrotoluene nitroso metabolite with thiols for toxic effects.

Toxicol Res (Camb) 2019 Mar 1;8(2):270-276. Epub 2019 Feb 1.

The 1st Affiliated Hospital of Dalian Medical University , 116000 Dalian , China . Email:

2,4,6-Trinitrotoluene () is a class C carcinogen as rated by the Environmental Protection Agency. One of the toxicity mechanisms of is the covalent binding of its metabolites to critical proteins. However, knowledge about their molecular reaction mechanisms is scarce. Read More

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http://dx.doi.org/10.1039/c8tx00326bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430087PMC

Ultrasensitive magnetic resonance imaging of systemic reactive oxygen species for early diagnosis of sepsis using activatable nanoprobes.

Chem Sci 2019 Apr 21;10(13):3770-3778. Epub 2019 Feb 21.

State Key Laboratory of Rare Earth Resources Utilization , Laboratory of Chemical Biology , Changchun Institute of Applied Chemistry , Chinese Academy of Sciences , Changchun , 130022 , P. R. China . Email: ; Email:

Current diagnostic methods for sepsis lack required speed or precision, often failing to make timely accurate diagnosis for early medical treatment. The systemic excess generation of reactive oxygen species (ROS) during sepsis has been considered as an early indicator of sepsis. Herein, we present the rational design of novel activatable nanoprobes (ROS CAs) composed of a clinically approved iron oxide core, Gd-DTPA, and hyaluronic acid (HA) that can image ROS down to sub-micromolar concentrations magnetic resonance imaging (MRI), and use them as sensitive contrast agents for sepsis evaluation. Read More

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http://dx.doi.org/10.1039/c8sc04961kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447818PMC

Making the unconventional μ-P bridging binding mode more conventional in phosphinine complexes.

Chem Sci 2019 Mar 21;10(11):3168-3180. Epub 2019 Jan 21.

Lehn Institute of Functional Materials (LIFM) , School of Chemistry , Sun Yat-Sen University , 510275 Guangzhou , China . Email:

Phosphinines, as aromatic heterocycles, usually engage in coordination as η-P σ-complexes or η-phosphinine π-complexes. The μ-P bridging coordination mode is rarely observed. With the aim to study the effect of different electronic configurations of phosphinines on the coordination modes, a series of anionic phosphinin-2-olates and neutral phosphinin-2-ols were prepared with moderate to high yield. Read More

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http://dx.doi.org/10.1039/c8sc05281fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427944PMC

Bioisosteres of Indomethacin as Inhibitors of Aldo-Keto Reductase 1C3.

ACS Med Chem Lett 2019 Apr 28;10(4):437-443. Epub 2019 Jan 28.

Department of Science and Drug Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, Italy.

Aldo-keto reductase 1C3 (AKR1C3) is an attractive target in drug design for its role in resistance to anticancer therapy. Several nonsteroidal anti-inflammatory drugs such as indomethacin are known to inhibit AKR1C3 in a nonselective manner because of COX-off target effects. Here we designed two indomethacin analogues by proposing a bioisosteric connection between the indomethacin carboxylic acid function and either hydroxyfurazan or hydroxy triazole rings. Read More

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http://dx.doi.org/10.1021/acsmedchemlett.8b00484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466513PMC

Feeling safe or unsafe in psychiatric inpatient care, a hospital-based qualitative interview study with inpatients in Sweden.

Int J Ment Health Syst 2019 8;13:23. Epub 2019 Apr 8.

1University Health Care Research Center, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Background: A major challenge in psychiatric inpatient care is to create an environment that promotes patient recovery, patient safety and good working environment for staff. Since guidelines and programs addressing this issue stress the importance of primary prevention in creating safe environments, more insight is needed regarding patient perceptions of feeling safe. The aim of this study is to enhance our understanding of feelings of being safe or unsafe in psychiatric inpatient care. Read More

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http://dx.doi.org/10.1186/s13033-019-0282-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452515PMC

Incidence and mortality of primary bone cancers in China, 2014.

Chin J Cancer Res 2019 Feb;31(1):135-143

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China.

Objective: In this study, we aimed to estimate the updated incidence and mortality of primary bone cancers based on population-based cancer registration data in 2014, collected by the National Central Cancer Registry of China (NCCRC).

Methods: In 2017, 339 registries' data were qualified based on data quality criteria set down by the NCCRC. Cases of primary bone cancers were retrieved from the national database. Read More

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http://dx.doi.org/10.21147/j.issn.1000-9604.2019.01.08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433577PMC
February 2019

Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling.

Sci Rep 2019 Apr 17;9(1):6199. Epub 2019 Apr 17.

Group of Applied Mechanics and Bioengineering (AMB), Aragón Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain.

The tumour microenvironment (TME) has recently drawn much attention due to its profound impact on tumour development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established in vitro models fail to include essential cues of the TME. Read More

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http://www.nature.com/articles/s41598-019-42529-8
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http://dx.doi.org/10.1038/s41598-019-42529-8DOI Listing
April 2019
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Autoimmune Pulmonary Alveolar Proteinosis Diagnosed After Exposure to a Fire Extinguisher Containing Silica Powder: A Case Study.

Intern Med 2019 Apr 17. Epub 2019 Apr 17.

Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, Japan.

We herein report a case of autoimmune pulmonary alveolar proteinosis (PAP) diagnosed after one-time exposure to silica powder. Owing to the misuse of a silica-containing fire extinguisher and the inhalation of large amounts of its powder, the patient experienced prolonged cough and visited our hospital. The findings of chest computed tomography and surgical lung biopsy specimens led to the diagnosis of PAP. Read More

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http://dx.doi.org/10.2169/internalmedicine.1557-18DOI Listing

UCP1 expression-associated gene signatures of human epicardial adipose tissue.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Department of Medicine, Faculty of Medicine, Université Laval, Québec, Québec, Canada.

Multiple reports of uncoupling protein 1 (UCP1) expression have established its presence in human epicardial adipose tissue (eAT). Its functional relevance to eAT, however, remains largely unknown. In a recent study, we reported that adrenergic stimulation of eAT was associated with downregulation of secreted proteins involved in oxidative stress-related and immune-related pathways. Read More

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http://dx.doi.org/10.1172/jci.insight.123618DOI Listing

Myelin repair stimulated by CNS-selective thyroid hormone action.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Department of Physiology & Pharmacology and Program in Chemical Biology, Oregon Health & Science University, Portland, Oregon, USA.

Oligodendrocyte processes wrap axons to form neuroprotective myelin sheaths, and damage to myelin in disorders, such as multiple sclerosis (MS), leads to neurodegeneration and disability. There are currently no approved treatments for MS that stimulate myelin repair. During development, thyroid hormone (TH) promotes myelination through enhancing oligodendrocyte differentiation; however, TH itself is unsuitable as a remyelination therapy due to adverse systemic effects. Read More

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http://dx.doi.org/10.1172/jci.insight.126329DOI Listing

Autophagy links antimicrobial activity with antigen presentation in Langerhans cells.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Division of Dermatology, Department of Medicine, and.

DC, through the uptake, processing, and presentation of antigen, are responsible for activation of T cell responses to defend the host against infection, yet it is not known if they can directly kill invading bacteria. Here, we studied in human leprosy, how Langerhans cells (LC), specialized DC, contribute to host defense against bacterial infection. IFN-γ treatment of LC isolated from human epidermis and infected with Mycobacterium leprae (M. Read More

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http://dx.doi.org/10.1172/jci.insight.126955DOI Listing

A glycopolymer improves vascoelasticity and mucociliary transport of abnormal cystic fibrosis mucus.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Department of Medicine, University of Alabama at Birmingham (UAB), Birmingham, Alabama, USA.

Cystic fibrosis (CF) is characterized by increased mucus viscosity and delayed mucociliary clearance that contributes to progressive decline of lung function. Mucus in the respiratory and GI tract is excessively adhesive in the presence of airway dehydration and excess extracellular Ca2+ upon mucin release, promoting hyperviscous, densely packed mucins characteristic of CF. Therapies that target mucins directly through ionic interactions remain unexploited. Read More

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http://dx.doi.org/10.1172/jci.insight.125954DOI Listing

DNA-encoded bispecific T cell engagers and antibodies present long-term antitumor activity.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.

Specific antibody therapy, including mAbs and bispecific T cell engagers (BiTEs), are important new tools for cancer immunotherapy. However, these approaches are slow to develop and may be limited in their production, thus restricting the patients who can access these treatments. BiTEs exhibit a particularly short half-life and difficult production. Read More

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https://insight.jci.org/articles/view/126086
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http://dx.doi.org/10.1172/jci.insight.126086DOI Listing
April 2019
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Classical and intermediate monocytes scavenge non-transferrin-bound iron and damaged erythrocytes.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Department of Internal Medicine II.

Myelomonocytic cells are critically involved in iron turnover as aged RBC recyclers. Human monocytes are divided in 3 subpopulations of classical, intermediate, and nonclassical cells, differing in inflammatory and migratory phenotype. Their functions in iron homeostasis are, however, unclear. Read More

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https://insight.jci.org/articles/view/98867
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http://dx.doi.org/10.1172/jci.insight.98867DOI Listing
April 2019
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Cinacalcet corrects biased allosteric modulation of CaSR by AHH autoantibody.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Department of Endocrinology and Nephrology, The University of Tokyo School of Medicine, Tokyo, Japan.

Biased agonism is a paradigm that may explain the selective activation of a signaling pathway via a GPCR that activates multiple signals. The autoantibody-induced inactivation of the calcium-sensing receptor (CaSR) causes acquired hypocalciuric hypercalcemia (AHH). Here, we describe an instructive case of AHH in which severe hypercalcemia was accompanied by an increased CaSR antibody titer. Read More

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http://dx.doi.org/10.1172/jci.insight.126449DOI Listing