179,277 results match your criteria Insight: The Journal of the American Society of Ophthalmic Registered Nurses [Journal]


Scalable Inference of Ordinary Differential Equation Models of Biochemical Processes.

Methods Mol Biol 2019 ;1883:385-422

Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany.

Ordinary differential equation models have become a standard tool for the mechanistic description of biochemical processes. If parameters are inferred from experimental data, such mechanistic models can provide accurate predictions about the behavior of latent variables or the process under new experimental conditions. Complementarily, inference of model structure can be used to identify the most plausible model structure from a set of candidates, and, thus, gain novel biological insight. Read More

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January 2019

Renal toxicity of heavy metals (cadmium and mercury) and their amelioration with ascorbic acid in rabbits.

Environ Sci Pollut Res Int 2018 Dec 13. Epub 2018 Dec 13.

Department of Veterinary Biomedical Sciences, PMAS Arid Agriculture University, Rawalpindi, PK-46300, Pakistan.

Cadmium and mercury are among the most toxic and dangerous environmental pollutants that may cause fatal implications. Vitamin C is an important chain-breaking antioxidant and enzyme co-factor against heavy metals. The objective of the present study was to evaluate the toxicological effects of cadmium chloride, mercuric chloride, and their co-administration on biochemical parameters of blood serum and metal bioaccumulation in kidneys and also to elucidate the protective effect of vitamin C in rabbits against these metals. Read More

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December 2018

Identification of potential genes involved in triterpenoid saponins biosynthesis in Gleditsia sinensis by transcriptome and metabolome analyses.

J Nat Med 2018 Dec 13. Epub 2018 Dec 13.

Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, Miyagi, 980-8577, Japan.

Gleditsia sinensis is widely used as a medicinal plant in Asia, especially in China. Triterpenes, alkaloids, and sterols were isolated from Gleditsia species. Among them, triterpenoid saponins are very important metabolites owing to their various pharmacological activities. Read More

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December 2018

Glucose metabolic brain patterns to discriminate amyotrophic lateral sclerosis from Parkinson plus syndromes.

EJNMMI Res 2018 Dec 13;8(1):110. Epub 2018 Dec 13.

Department of Nuclear Medicine and Molecular Imaging, Division of Nuclear Medicine, KU Leuven, Herestraat 49, 3000, Leuven, Belgium.

Background: F-FDG brain PET measures metabolic changes in neurodegenerative disorders and may discriminate between different diseases even at an early stage. The objective of this study was to classify patients with amyotrophic lateral sclerosis (ALS) and Parkinson plus syndromes (PP). To this end, different approaches were evaluated using generalized linear models and corresponding glucose metabolic brain patterns. Read More

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December 2018

Microarray, IPA and GSEA Analysis in Mice Models.

Bio Protoc 2018 Sep;8(17)

Department of Animal Sciences, Purdue University, West Lafayette, United States.

This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety of processes across different tissue samples, conditions, and the like. The full method detailed below can be applied to determine the effects of muscle-specific Notch1 activation in the mouse model and to analyze previously published microarray data of human liposarcoma cell lines. Read More

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September 2018

The author identified by his method: EuPA YPIC challenge solved.

EuPA Open Proteom 2018 Dec 13;20:1-8. Epub 2018 Oct 13.

Moscow Institute of Physics and Technology (State University), Moscow, Russia.

Here we present the results of our attempt on the EuPA YPIC challenge. The task was to sequence the provided synthetic peptides, build the sentence encrypted in them and determine from which book the sentence originated. The task itself, while holding no direct scientific value, offers an insight in less formal terms (for participants at least) on how the overall process of a scientific study of a "new protein" looks like. Read More

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December 2018

Bone marrow endothelial cells sustain a tumor-specific CD8 T cell subset with suppressive function in myeloma patients.

Oncoimmunology 2019 22;8(1):e1486949. Epub 2018 Oct 22.

Department of Biomedical Sciences and Human Oncology, Unit of Internal Medicine "Guido Baccelli", University of Bari Medical School, Bari, Italy.

Endothelial cells (EC) line the bone marrow microvasculature and are in close contact with CD8 T cells that come and go across the permeable capillaries. Because of these intimate interactions, we investigated the capacity of EC to act as antigen-presenting cells (APC) and modulate CD8 T cell activation and proliferation in bone marrow of patients with multiple myeloma (MM) and monoclonal gammopathy of undetermined significance. We found that EC from MM patients show a phenotype of semi-professional APC given that they express low levels of the co-stimulatory molecules CD40, CD80 and CD86, and of the inducible co-stimulator ligand (ICOSL). Read More

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October 2018

Genetic associations of T cell cancer immune response with tumor aggressiveness in localized prostate cancer patients and disease reclassification in an active surveillance cohort.

Oncoimmunology 2019 29;8(1):e1483303. Epub 2018 Oct 29.

Departments of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Determining prostate cancer (PCa) aggressiveness and reclassification are critical events during the treatment of localized disease and for patients undergoing active surveillance (AS). Since T cells play major roles in cancer surveillance and elimination, we aimed to identify genetic biomarkers related to T cell cancer immune response which are predictive of aggressiveness and reclassification risks in localized PCa. The genotypes of 3,586 single nucleotide polymorphisms (SNPs) from T cell cancer immune response pathways were analyzed in 1762 patients with localized disease and 393 who elected AS. Read More

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October 2018

Identification of key genes and pathways by bioinformatics analysis with TCGA RNA sequencing data in hepatocellular carcinoma.

Mol Clin Oncol 2018 Dec 27;9(6):597-606. Epub 2018 Sep 27.

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

Improved insight into the molecular characteristics of hepatocellular carcinoma (HCC) is required to predict prognosis and to develop a new rationale for targeted therapeutic strategy. Bioinformatics methods, including functional enrichment and network analysis combined with survival analysis, are required to process a large volume of data to obtain further information on differentially expressed genes (DEGs). The RNA sequencing data related to HCC in The Cancer Genome Atlas (TCGA) database were analyzed to screen DEGs, which were separately submitted to perform gene enrichment analysis to identify gene sets and signaling pathways, and to construct a protein-protein interaction (PPI) network. Read More

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December 2018

Frequent activating STAT3 mutations and novel recurrent genomic abnormalities detected in breast implant-associated anaplastic large cell lymphoma.

Oncotarget 2018 Nov 16;9(90):36126-36136. Epub 2018 Nov 16.

Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a rare form of T-cell lymphoma that occurs after implantation of breast prostheses. We performed comprehensive next generation sequencing based genomic characterization of 11 cases of BIA-ALCL including sequence variant detection on 180 genes frequently mutated in haematological malignancy, genome-wide copy number assessment, structural variant detection involving the T-cell receptor loci and TRB deep-sequencing. We observed sequence variants leading to JAK/STAT activation in 10 out of 11 patients. Read More

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November 2018

Novel insight into the function of tankyrase.

Authors:
Mi Kyung Kim

Oncol Lett 2018 Dec 5;16(6):6895-6902. Epub 2018 Oct 5.

Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

Tankyrases are multifunctional poly(ADP-ribose) polymerases that regulate a variety of cellular processes, including Wnt signaling, telomere maintenance and mitosis regulation. Tankyrases interact with target proteins and regulate their interactions and stability through poly(ADP-ribosyl) ation. In addition to their roles in telomere maintenance and regulation of mitosis, tankyrase proteins regulate tumor suppressors, including AXIN, phosphatase and tensin homolog and angiomotin. Read More

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December 2018

Mapping the interview transcript: Identifying spatial policy areas from daily working practices.

Area (Oxf) 2018 Dec 24;50(4):529-541. Epub 2017 Nov 24.

School of Geography and Planning Cardiff University Cardiff UK.

An interview transcript can be a rich source of geographical references whose potential are not always fully realised in their conventional analysis. Geo-referencing techniques can be used to assign a spatial footprint to place names, adding value to these data and allowing the geographic information within them to be exploited when coupled with GIS technology. This paper discusses a method of analysing and visualising interview transcripts in order to understand the spatial extent of public policy practitioners' activities. Read More

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December 2018
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Author Correction: New insight for pharmacogenomics studies from the transcriptional analysis of two large-scale cancer cell line panels.

Sci Rep 2018 Dec 13;8(1):17945. Epub 2018 Dec 13.

Residual Tumor & Response to Treatment Laboratory (RT2Lab), PSL Research University, Translational Research Department, F-75248, Paris, France.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

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December 2018

Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells.

Sci Rep 2018 Dec 13;8(1):17818. Epub 2018 Dec 13.

Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, R3T 2N2, Canada.

In mammalian cells cytoplasm ion concentrations and hence cytoplasm conductivity is an important indicator of their physiological state. Changes in the cytoplasm conductivity has been associated with physiological changes such as progression of cancer and apoptosis. In this work, a model that predicts the effects of physiological changes in ion transport on the cytoplasm conductivity of Chinese hamster ovary (CHO) cells is demonstrated. Read More

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December 2018

Clinical trial design and new therapies for pulmonary arterial hypertension.

Eur Respir J 2018 Dec 13. Epub 2018 Dec 13.

San Diego School of Medicine, University of California, La Jolla, CA, USA.

Until 20 years ago the treatment of pulmonary arterial hypertension (PAH) was based on case reports and small series, and was largely ineffectual. As a deeper understanding of the pathogenesis and pathophysiology of PAH evolved over the subsequent two decades, coupled with epidemiological studies defining the clinical and demographic characteristics of the condition, a renewed interest in treatment development emerged through collaborations between international experts, industry and regulatory agencies. These efforts led to the performance of robust, high-quality clinical trials of novel therapies that targeted putative pathogenic pathways, leading to the approval of more than 10 novel therapies that have beneficially impacted both the quality and duration of life. Read More

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December 2018
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High-affinity allergen-specific human antibodies cloned from single IgE B cell transcriptomes.

Science 2018 Dec;362(6420):1306-1309

Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.

Immunoglobulin E (IgE) antibodies protect against helminth infections but can also cause life-threatening allergic reactions. Despite their role in human health, the cells that produce these antibodies are rarely observed and remain enigmatic. We isolated single IgE B cells from individuals with food allergies and used single-cell RNA sequencing to elucidate the gene expression and splicing patterns unique to these cells. Read More

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December 2018
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Transcriptome-wide isoform-level dysregulation in ASD, schizophrenia, and bipolar disorder.

Science 2018 Dec;362(6420)

Department of Psychiatry, Semel Institute, David Geffen School of Medicine, University of California, Los Angeles, 695 Charles E. Young Drive South, Los Angeles, CA 90095, USA.

Most genetic risk for psychiatric disease lies in regulatory regions, implicating pathogenic dysregulation of gene expression and splicing. However, comprehensive assessments of transcriptomic organization in diseased brains are limited. In this work, we integrated genotypes and RNA sequencing in brain samples from 1695 individuals with autism spectrum disorder (ASD), schizophrenia, and bipolar disorder, as well as controls. Read More

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December 2018
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Effect of gap junction-mediated intercellular communication on TGF-β induced epithelial-to-mesenchymal transition.

Biochem Biophys Res Commun 2018 Dec 10. Epub 2018 Dec 10.

Department of Cellular Physiological Chemistry, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan. Electronic address:

Epithelial-to-mesenchymal transition (EMT) is the process in which epithelial cells lose cell polarity and cell adhesion with surrounding cells to obtain migratory and invasive abilities. On the other hand, the expression of connexin is decreased or lacked in the many types of tumor cells. This study examined the effect of gap junctional intercellular communication (GJIC) on EMT induced by the transforming growth factor-β1 (TGF-β1). Read More

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December 2018

Host-specific expression of Ixodes scapularis salivary genes.

Ticks Tick Borne Dis 2018 Dec 3. Epub 2018 Dec 3.

Section of Infectious Diseases, New Haven, CT, 06520, USA; Howard Hughes Medical Institute, Chevy Chase, Maryland, 20815, USA.

Ixodes scapularis vectors several pathogens including Borrelia burgdorferi, the agent of Lyme disease. Nymphal and larval stages, and the pathogens transmitted by I. scapularis are maintained in a zoonotic cycle involving rodent reservoir hosts, predominantly Peromyscus leucopus. Read More

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December 2018

Using a birth cohort to study brain health and preclinical dementia: recruitment and participation rates in Insight 46.

BMC Res Notes 2018 Dec 13;11(1):885. Epub 2018 Dec 13.

MRC Unit for Lifelong Health and Ageing at UCL, London, UK.

Objective: Identifying and recruiting people with early pre-symptomatic Alzheimer's disease to neuroimaging research studies is increasingly important. The extent to which results of these studies can be generalised depends on the recruitment and representativeness of the participants involved. We now report the recruitment and participation patterns from a neuroscience sub-study of the MRC National Survey of Health and Development, "Insight 46". Read More

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December 2018

Comparative genomic analysis revealed rapid differentiation in the pathogenicity-related gene repertoires between Pyricularia oryzae and Pyricularia penniseti isolated from a Pennisetum grass.

BMC Genomics 2018 Dec 13;19(1):927. Epub 2018 Dec 13.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: A number of Pyricularia species are known to infect different grass species. In the case of Pyricularia oryzae (syn. Magnaporthe oryzae), distinct populations are known to be adapted to a wide variety of grass hosts, including rice, wheat and many other grasses. Read More

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December 2018

Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions.

BMC Cell Biol 2018 Dec 13;19(1):27. Epub 2018 Dec 13.

Department of Rheumatology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Pvovince, China.

Background: Within the past years, umbilical cord (UC) and amniotic membrane (AM) expanded in human platelet lysate (PL) have been found to become increasingly candidate of mesenchymal stromal cells (MSCs) in preclinical and clinical studies. Different sources of MSCs have different properties, and lead to different therapeutic applications. However, the similarity and differences between the AMMSCs and UCMSCs in PL remain unclear. Read More

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December 2018

New Insight into Sperm Capacitation: A Novel Mechanism of 17β-Estradiol Signalling.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Laboratory of Reproductive Biology, Institute of Biotechnology CAS, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic.

17β-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation-called capacitation-and sperm⁻egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Read More

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December 2018

Tannic Acid-Induced Surface-Catalyzed Secondary Nucleation during the Amyloid Fibrillation of Hen Egg-White Lysozyme.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.

Amyloid fibrillation by hen egg white lysozyme under the influence of tannic acid was investigated by atomic force microscopy and fluorescence spectroscopy. Tannic acid was found to be able to induce the formation of amyloid fibrils with an interesting mixed morphology. Such morphology features with the existence of areas of thickening alternating with areas of normal height. Read More

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December 2018

The Impact of Protein Acetylation/Deacetylation on Systemic Lupus Erythematosus.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA.

Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease in which the body's immune system mistakenly attacks healthy cells. Although the exact cause of SLE has not been identified, it is clear that both genetics and environmental factors trigger the disease. Identical twins have a 24% chance of getting lupus disease if the other one is affected. Read More

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December 2018
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Clinical Significance of CUB and Sushi Multiple Domains 1 Inactivation in Head and Neck Squamous Cell Carcinoma.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Department of Otolaryngology-Head and Neck Surgery, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 150, Seongan-ro Gangdong-Gu, Seoul 134-701, Korea.

Although the genetic alteration of CUB and Sushi multiple domains 1 (CSMD1) is known to be associated with poor prognosis in several cancers, there is a lack of clinical relevance in head and neck cancer. The aim of this study was to offer insight into the clinical significance of CSMD1, utilizing a multimodal approach that leverages publicly available independent genome-wide expression datasets. CSMD1-related genes were found and analyzed to examine the clinical significance of CSMD1 inactivation in the HNSCC cohort of publicly available databases. Read More

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December 2018

Gut Microbiota, Fusobacteria, and Colorectal Cancer.

Diseases 2018 Dec 11;6(4). Epub 2018 Dec 11.

Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.

The gut microbiota has emerged as an environmental contributor to colorectal cancer (CRC) in both animal models and human studies. It is now generally accepted that bacteria are ubiquitous colonizers of all exposed human body surfaces, including the entire alimentary tract (5). Recently, the concept that a normal bacterial microbiota is essential for the development of inflammation-induced carcinoma has emerged from studies of well-known colonic bacterial microbiota. Read More

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December 2018

A Ruthenium(II) N-Heterocyclic Carbene (NHC) Complex with Naphthalimide Ligand Triggers Apoptosis in Colorectal Cancer Cells via Activating the ROS-p38 MAPK Pathway.

Int J Mol Sci 2018 Dec 9;19(12). Epub 2018 Dec 9.

Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.

The p38 MAPK pathway is known to influence the anti-tumor effects of several chemotherapeutics, including that of organometallic drugs. Previous studies have demonstrated the important role of p38 both as a regulator and a sensor of cellular reactive oxygen species (ROS) levels. Investigating the anti-cancer properties of novel 1,8-naphthalimide derivatives containing Rh(I) and Ru(II) -heterocyclic carbene (NHC) ligands, we observed a profound induction of ROS by the complexes, which is most likely generated from mitochondria (mtROS). Read More

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December 2018

From Synapse to Function: A Perspective on the Role of Neuroproteomics in Elucidating Mechanisms of Drug Addiction.

Proteomes 2018 Dec 9;6(4). Epub 2018 Dec 9.

Department of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA.

Drug addiction is a complex disorder driven by dysregulation in molecular signaling across several different brain regions. Limited therapeutic options currently exist for treating drug addiction and related psychiatric disorders in clinical populations, largely due to our incomplete understanding of the molecular pathways that influence addiction pathology. Recent work provides strong evidence that addiction-related behaviors emerge from the convergence of many subtle changes in molecular signaling networks that include neuropeptides (neuropeptidome), protein-protein interactions (interactome) and post-translational modifications such as protein phosphorylation (phosphoproteome). Read More

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December 2018

Mitochondrial Membrane Dynamics-Functional Positioning of OPA1.

Antioxidants (Basel) 2018 Dec 8;7(12). Epub 2018 Dec 8.

Department of Physiology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.

The maintenance of mitochondrial energetics requires the proper regulation of mitochondrial morphology, and vice versa. Mitochondrial dynamins control mitochondrial morphology by mediating fission and fusion. One of them, optic atrophy 1 (OPA1), is the mitochondrial inner membrane remodeling protein. Read More

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December 2018

Decreases Endothelial Damage and Oxidative Stress Indicators in Patients with Systemic Arterial Hypertension: Results from Exploratory Controlled Clinical Trial.

Mar Drugs 2018 Dec 8;16(12). Epub 2018 Dec 8.

Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico.

(1) Background: () has shown beneficial effects such as being anti-dyslipidemic, antiviral, antioxidant and antihypertensive. However, there are few and limited clinical studies. (2) Methods: a prospective, randomized, parallel pilot study of 4. Read More

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December 2018

An Evolutionary Insight into Zika Virus Strains Isolated in the Latin American Region.

Viruses 2018 Dec 8;10(12). Epub 2018 Dec 8.

Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay.

Zika virus (ZIKV) is an emerging pathogen member of the family. ZIKV has spread rapidly in the Latin American region, causing hundreds of thousands of cases of ZIKV disease, as well as microcephaly in congenital infections. Detailed studies on the pattern of evolution of ZIKV strains have been extremely important to our understanding of viral survival, fitness, and evasion of the host's immune system. Read More

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December 2018

Effects of Rich-Polyphenols Extract of Dendrobium loddigesii on Anti-Diabetic, Anti-Inflammatory, Anti-Oxidant, and Gut Microbiota Modulation in db/db Mice.

Molecules 2018 Dec 7;23(12). Epub 2018 Dec 7.

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.

is a traditional Chinese herb with anti-diabetic effects and has diverse bibenzyls as well as phenanthrenes. Little is known about polyphenols anti-diabetic activities, so, a rich-polyphenols extract of (DJP) was used for treatment of diabetic db/db mice; the serum biochemical index and tissue appearance were evaluated. In order to gain an insight into the anti-diabetic mechanism, the oxidative stress index, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and gut microbiota modulation were determined by ELISA, immunohistochemistry or high throughput sequencing 16S rRNA gene. Read More

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December 2018

MET/HGF Co-Targeting in Pancreatic Cancer: A Tool to Provide Insight into the Tumor/Stroma Crosstalk.

Int J Mol Sci 2018 Dec 7;19(12). Epub 2018 Dec 7.

Department of Oncology, University of Torino, 10060 Candiolo, Italy.

The 'onco-receptor' MET (Hepatocyte Growth Factor Receptor) is involved in the activation of the invasive growth program that is essential during embryonic development and critical for wound healing and organ regeneration during adult life. When aberrantly activated, MET and its stroma-secreted ligand HGF (Hepatocyte Growth Factor) concur to tumor onset, progression, and metastasis in solid tumors, thus representing a relevant target for cancer precision medicine. In the vast majority of tumors, wild-type behaves as a 'stress-response' gene, and relies on ligand stimulation to sustain cancer cell 'scattering', invasion, and protection form apoptosis. Read More

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December 2018

An in silico analysis of human sperm genes associated with asthenozoospermia and its implication in male infertility.

Medicine (Baltimore) 2018 Dec;97(49):e13338

Central Laboratory.

Asthenozoospermia is the most common clinical symptom of male infertility. Molecular markers associated with asthenozoospermia spermatozoa are scarcely identified. The objective of this study was to screen the differentially expressed genes (DEGs) in asthenozoospermia spermatozoa and assess the underlying bioinformatics roles in regulation of sperm quality. Read More

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December 2018

Psychosocial consequences of living kidney donation: a prospective multicentre study on health-related quality of life, donor-recipient relationships and regret.

Nephrol Dial Transplant 2018 Dec 13. Epub 2018 Dec 13.

Institute of Psychology, Health, Medical and Neuropsychology Unit, Leiden University, Leiden, The Netherlands.

Background: Previous studies have indicated decreased health-related quality of life (HRQoL) shortly after kidney donation, returning to baseline in the longer term. However, a subgroup of donors experiences persistent HRQoL problems. To identify which HRQoL aspects are impacted most by the donation and to identify at-risk donors, more specific insight into psychosocial donation consequences is needed. Read More

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December 2018

Molecular mechanisms in skeletal muscle underlying insulin resistance in lean women with polycystic ovary syndrome.

J Clin Endocrinol Metab 2018 Dec 13. Epub 2018 Dec 13.

Molecular Physiology Section, Department of Nutrition, Exercise and Sports, University of Copenhagen, Denmark.

Context: Skeletal muscle molecular mechanisms underlying insulin resistance in women with polycystic ovary syndrome (PCOS) are poorly understood.

Objective: To provide insight into mechanisms regulating skeletal muscle insulin resistance in lean women with PCOS.

Participants And Methods: A hyperinsulinemic-euglycemic clamp with skeletal muscle biopsies was performed. Read More

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December 2018

Calcium imaging, MEA recordings and Immunostaining images dataset of Neuron-Astrocyte Networks in Culture under the Effect of Norepinephrine.

Gigascience 2018 Dec 13. Epub 2018 Dec 13.

School of Electrical Engineering, Tel-Aviv University, Tel-Aviv, Israel.

Background: Monitoring the activity and morphology of Neuron-astrocyte networks in culture is powerful tool for studying dynamics, structure and communication in neuron-astrocyte networks independently or as a model of sub-brain network. These cultures are known to produce stereotypical patterns of activity e.g. Read More

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December 2018

Insight into the role of TRAIL in liver diseases.

Biomed Pharmacother 2018 Dec 10;110:641-645. Epub 2018 Dec 10.

Center of Infectious Diseases, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang, Wuhou District, Chengdu, Sichuan Province, China. Electronic address:

TNF-related apoptosis inducing ligand (TRAIL) is a potential antitumor protein known for its ability to selectively eliminate various types of tumor cells without exerting toxic effects in normal cells and tissues. TRAIL has recently been suggested as a potential therapeutic target in hepatocellular carcinoma (HCC) because it promotes apoptosis in cancer cells. Furthermore, studies on the role of TRAIL in liver injury have reported that TRAIL plays an essential role in viral hepatitis, fatty liver diseases, etc. Read More

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December 2018

The loss of tolerance to CHI3L1 - A putative role in inflammatory bowel disease?

Clin Immunol 2018 Dec 10. Epub 2018 Dec 10.

Institute for Biotechnology, Faculty of Environment and Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968 Senftenberg, Germany. Electronic address:

The incidence of inflammatory bowel disease (IBD) is steadily increasing. IBD is characterized by a chronic inflammation of the gastrointestinal tract and is classified into the two main groups Crohn's disease (CD) and ulcerative colitis (UC). Genetic predispositions, environmental factors and a dysregulated immune response are known to part take in the onset of IBD. Read More

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December 2018

Di-2-ethylhexylphthalate promotes thyroid cell proliferation and DNA damage through activating thyrotropin-receptor-mediated pathways in vitro and in vivo.

Food Chem Toxicol 2018 Dec 10. Epub 2018 Dec 10.

Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea. Electronic address:

Phthalates are being suggested to be associated with altered thyroid function and proliferative changes, but detailed mechanisms remain unclear. Here, we examined the effects of di-(2-ethylhexyl) phthalate (DEHP) on DNA damage and proliferation in thyroid using thyroid carcinoma cell line, 8505C, in vitro and the rats orally treated with DEHP at 0, 0.3, 3, 30 and 150 mg/kg for 90 days from post-natal day 9 in vivo. Read More

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December 2018

Profiling of carboxyl-containing metabolites in smokers and non-smokers by stable isotope labeling combined with LC-MS/MS.

Anal Biochem 2018 Dec 10. Epub 2018 Dec 10.

China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China. Electronic address:

Profiling of carboxyl-containing metabolites in smokers and non-smokers provides insight into the smoking-related biological events and causal relationships between exposure and adverse events. However, more comprehensive analysis of carboxyl-containing metabolites in bio-matrices with high sensitivity and accuracy is challenging. In this work, stable isotope labeling in combination with liquid chromatography-tandem mass spectrometry was used for untargeted profiling and relative quantification of carboxyl-containing metabolites in plasma of smokers and non-smokers. Read More

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December 2018

In vitro lipid transfer assays of phosphatidylinositol transfer proteins provide insight into the in vivo mechanism of ligand transfer.

Biochim Biophys Acta Biomembr 2018 Dec 10. Epub 2018 Dec 10.

Department of Chemistry and Centre for Biotechnology, Brock University, St. Catharines, Ontario L2A 3S1, Canada. Electronic address:

Fluorescence resonance energy transfer (FRET) assays and membrane binding determinations were performed using three phosphatidylinositol transfer proteins, including the yeast Sec14 and two mammalian proteins PITPα and PITPβ. These proteins were able to specifically bind the fluorescent phosphatidylcholine analogue NBD-PC ((2-(12-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine)) and to transfer it to small unilamellar vesicles (SUVs). Rate constants for transfer to vesicles comprising 100% PC were slower for all proteins than when increasing percentages of phosphatidylinositol were incorporated into the same SUVs. Read More

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December 2018

Guinea pig immunoglobulin VH and VL naïve repertoire analysis.

PLoS One 2018 13;13(12):e0208977. Epub 2018 Dec 13.

Laboratory of Molecular and Cellular Biology, Graduate School of Science and Engineering for Research, University of Toyama, Toyama-shi, Toyama, Japan.

The guinea pig has been used as a model to study various human infectious diseases because of its similarity to humans regarding symptoms and immune response, but little is known about the humoral immune response. To better understand the mechanism underlying the generation of the antibody repertoire in guinea pigs, we performed deep sequencing of full-length immunoglobulin variable chains from naïve B and plasma cells. We gathered and analyzed nearly 16,000 full-length VH, Vκ and Vλ genes and analyzed V and J gene segment usage profiles and mutation statuses by annotating recently reported genome data of guinea pig immunoglobulin genes. Read More

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December 2018

Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid.

Med Sci Monit 2018 Dec 13;24:9032-9044. Epub 2018 Dec 13.

Institute of Burns, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).

BACKGROUND Hedgehog (Hh) signaling pathway-related genes have important roles in several physiological and disease processes that involve cell proliferation. Long non-coding region RNAs (lncRNAs) have a regulatory role on gene expression. Keloid is characterized by excessive proliferation of scar tissue following trauma. Read More

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December 2018

Relationships Among Fatigue, Anxiety, Depression, and Pain and Health-Promoting Lifestyle Behaviors in Women With Early-Stage Breast Cancer.

Cancer Nurs 2018 Dec 12. Epub 2018 Dec 12.

Author Affiliations: Department of Biobehavioral Nursing Science, College of Nursing, University of Florida, Gainesville (Drs Lynch Kelly, Alexander-Delpech, Lyon, Siangphoe, and Yang); School of Nursing (Dr. Starkweather), University of Connecticut, Storrs.

Background: With a nearly 89% 5-year survival rate for women with early-stage breast cancer, symptoms are a priority. Healthy lifestyle behaviors may be temporally associated with symptoms; however, evidence is lacking.

Objective: This research examined temporal relationships among healthy lifestyle behaviors and symptoms in women diagnosed with breast cancer receiving chemotherapy. Read More

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December 2018

Next Generation Sequencing in Molecular Diagnosis of Lynch Syndrome - a Pilot Study Using New Stratification Criteria.

Acta Medica (Hradec Kralove) 2018 ;61(3):98-102

Department of Molecular Biology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Slovakia.

The development of the new technologies such as the next-generation sequencing (NGS) makes more accessible the diagnosis of genetically heterogeneous diseases such as Lynch syndrome (LS). LS is one of the most common hereditary form of colorectal cancer. This autosomal dominant inherited disorder is caused by deleterious germline mutations in one of the mismatch repair (MMR) genes - MLH1, MSH2, MSH6 or PMS2, or the deletion in the EPCAM gene. Read More

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January 2018

Pharyngo-Esophageal and Cardio-Respiratory Interactions: Potential Implications for Premature Infants at-risk of Clinically Significant Cardiorespiratory Events.

Am J Physiol Gastrointest Liver Physiol 2018 Dec 13. Epub 2018 Dec 13.

Section of Neonatology-Pediatric Gastroenterology, The Research Institute at Nationwide Children's Hospital, United States.

Objective: Aims were to: 1) examine pharyngo-esophageal and cardio-respiratory responses to provoking pharyngeal stimuli, and 2) determine potential contributory factors impacting heart rate (HR) changes to provide insight into cardio-respiratory events occurring in preterm infants.

Methods: Forty-eight neonates (29 males, born at 27.7 ± 0. Read More

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December 2018

Matched / Mismatched Cases in Proline-Catalyzed Cascade Reactions with Carbohydrates - a Computational Insight into the Role of D- and L-Proline.

J Org Chem 2018 Dec 13. Epub 2018 Dec 13.

The cascade reactions of carbohydrates with methyl ketones in the presence of proline feature complex running reaction steps. By extensive quantum mechanical simulation a coherent reaction mechanism was identified matching the experimental data. The present calculations indicate a Mannich reaction / proline hydrolysis / retro aza-Michael cascade to form an intermediate α,β-unsaturated ethyl ketone. Read More

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December 2018

Insight into the Dynamics of Different Fluorophores in the Interior of Aerosol OT Lamellar Structures in Presence of Sugars: From Picosecond-to-Femtosecond Study.

J Phys Chem B 2018 Dec 13. Epub 2018 Dec 13.

It is well reported that sugar molecules provide different types of stabilization to the biomembranes both in vitro as well as in vivo. In the present article, our focus is to investigate the interactions of two sugar molecules (sucrose and sucralose) with the lamellar structures of aerosol OT (AOT). We have attempted the structural characterization of the lamellae in presence and absence of the sugars with the aid of dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), Fourier transformed infrared spectroscopy (FTIR), polarized optical microscopy (POM) and cryogenic-transmission electron microscopic (Cryo-TEM) techniques. Read More

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December 2018