992,737 results match your criteria In Vitro Cellular & Developmental Biology - Animal [Journal]


In silico prediction of short hairpin RNA and in vitro silencing of activin receptor type IIB in chicken embryo fibroblasts by RNA interference.

Mol Biol Rep 2019 Mar 16. Epub 2019 Mar 16.

Division of Animal Genetics & Breeding, Indian Veterinary Research Institute, Izatnagar, U.P., India.

Gene silencing by RNA interference is extensively used reverse genetic approach to analyse the implications of any gene in mammalian systems. The silencing of the Activin type IIB receptor belonging to transforming growth factor beta superfamily has demonstrated increase in muscle growth in many species. We designed five short hairpin RNA constructs targeting coding region of chicken ACTRIIB. Read More

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http://link.springer.com/10.1007/s11033-019-04756-0
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http://dx.doi.org/10.1007/s11033-019-04756-0DOI Listing
March 2019
1 Read

Individual and combined toxicity of carboxylic acid functionalized multi-walled carbon nanotubes and benzo a pyrene in lung adenocarcinoma cells.

Environ Sci Pollut Res Int 2019 Mar 16. Epub 2019 Mar 16.

School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Co-exposure to carboxylic acid functionalized multi-walled carbon nanotubes (F-MWCNTs) and polycyclic aromatic hydrocarbons (PAHs) such as benzo a pyrene (BaP) in ambient air have been reported. Adsorption of BaP to F-MWCNTs can influence combined toxicity. Studying individual toxicity of F-MWCNTs and BaP might give unrealistic data. Read More

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http://dx.doi.org/10.1007/s11356-019-04795-xDOI Listing

Induction of p53-mediated senescence is essential for the eventual anticancer therapeutic effect of RH1.

Arch Pharm Res 2019 Mar 16. Epub 2019 Mar 16.

Institute for Innovative Cancer Research, Asan Medical Center, Seoul, 05505, Korea.

RH1 (2, 5-diaziridinyl-3-(hydroxymethy)-6-methyl-1, 4-benzoquinone) is a bioreductive anticancer drug. The mechanism underlying its therapeutic properties has not yet been elucidated. In this study, we aimed to determine whether RH1 exerts its anticancer effect via p53-mediated apoptosis and senescence in vitro and in vivo. Read More

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http://dx.doi.org/10.1007/s12272-019-01132-1DOI Listing

The roles of FGF21 in atherosclerosis pathogenesis.

Rev Endocr Metab Disord 2019 Mar 16. Epub 2019 Mar 16.

Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran.

FGF21 is a peptide hormone that regulates homeostasis of lipid and glucose as well as energy metabolism. It is mainly expressed and secreted in liver and adipose tissues, and it is expressed in lower amounts in the aorta. Recent clinical and preclinical studies indicate increased serum FGF21 levels in atherosclerosis patients. Read More

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http://dx.doi.org/10.1007/s11154-019-09488-xDOI Listing

Presence of vasculature results in faster insulin response in adipocytes in vascularized adipose tissue model.

ALTEX 2019 Mar 11. Epub 2019 Mar 11.

Cell Biology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.

Besides being an energy storage, adipose tissue is an endocrine organ closely associated with vascular system. Human relevant in vitro models are needed to study adipose tissue and related diseases. Vasculature plays a central role in the development and inhibition of adipose tissue related diseases. Read More

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http://dx.doi.org/10.14573/altex.1811271DOI Listing

Heterogeneous clinical phenotypes and cerebral malformations reflected by rotatin cellular dynamics.

Brain 2019 Mar 16. Epub 2019 Mar 16.

Department of Clinical Genetics, Erasmus University Medical Center (Erasmus MC), P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.

Recessive mutations in RTTN, encoding the protein rotatin, were originally identified as cause of polymicrogyria, a cortical malformation. With time, a wide variety of other brain malformations has been ascribed to RTTN mutations, including primary microcephaly. Rotatin is a centrosomal protein possibly involved in centriolar elongation and ciliogenesis. Read More

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http://dx.doi.org/10.1093/brain/awz045DOI Listing

Murrayanine Induces Cell Cycle Arrest, Oxidative Stress, and Inhibition of Phosphorylated p38 Expression in A549 Lung Adenocarcinoma Cells.

Med Sci Monit 2019 Mar 17;25:2002-2008. Epub 2019 Mar 17.

Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao, Shandong, China (mainland).

BACKGROUND Murrayanine is a carbazole alkaloid derived from Murraya koenigii, which has been used in traditional Chinese medicine in the treatment of cancer. This study aimed to investigate the effects of murrayanine on human lung adenocarcinoma cells in vitro and to investigate the mechanisms of its action. MATERIAL AND METHODS A549 human lung adenocarcinoma cells and MRC-5 human lung fibroblasts were grown in culture, and an MTT assay determined cell viability. Read More

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http://dx.doi.org/10.12659/MSM.913873DOI Listing

The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways.

Phytomedicine 2019 Feb 18;58:152864. Epub 2019 Feb 18.

School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, 30 Qingquan Road of Laishan District, Yantai 264003, Shandong, PR China. Electronic address:

Background: Ulcerative colitis (UC) is a chronic inflammatory condition of the intestines and is difficult to cure once diagnosed. The efficacy of the current clinical treatment for UC is limited. Common anti-inflammatory drugs are prone to adverse effects, while novel biological agents are expensive, although tolerated by patients. Read More

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http://dx.doi.org/10.1016/j.phymed.2019.152864DOI Listing
February 2019

Evaluation of synthetic substituted 1,2-dioxanes as novel agents against human leishmaniasis.

Eur J Med Chem 2019 Mar 3;170:126-140. Epub 2019 Mar 3.

Alma Mater Studiorum - University of Bologna, Department of Chemistry "G. Ciamician", Via Selmi 2, 40126, Bologna, Italy.

The treatment of human leishmaniasis is currently based on few compounds that are highly toxic, expensive and have a high rate of treatment failure. A number of recent studies on new drugs focuses on natural or semi-synthetic compounds. Among them, the endoperoxide artemisinin, extracted from Artemisia annua, and some of its derivatives have shown leishmanicidal activity. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.02.070DOI Listing

Supramolecular nanoassembly of lysozyme and α-lactalbumin (apo α-LA) exhibits selective cytotoxicity and enhanced bioavailability of curcumin to cancer cells.

Colloids Surf B Biointerfaces 2019 Mar 8;178:297-306. Epub 2019 Mar 8.

Structural Biology and Nanomedicine Laboratory, Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, 769008, Odisha, India. Electronic address:

Hybrid supramolecular spherical nanoassembly of hen egg white lysozyme and bovine apo α lactalbumin (SN) was prepared with a mean size of ˜55.2 nm using an optimized desolvation method via chemical crosslinking. The nanoassembly, SN demonstrated dose-dependent reactive oxygen species (ROS) mediated cytotoxicity in multiple cancer cells such as MCF-7, MDA-MB231, HeLa and MG 63. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.03.016DOI Listing

A Novel Gastric Spheroid Co-Culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium.

Cell Mol Gastroenterol Hepatol 2019 Mar 13. Epub 2019 Mar 13.

Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA. Electronic address:

Background & Aims: Gastric dendritic cells (DCs) control the adaptive response to infection with Helicobacter pylori, a major risk factor for peptic ulcer disease and gastric cancer. We hypothesize that DC interactions with the gastric epithelium position gastric DCs for uptake of luminal H. pylori and promote DC responses to epithelial-derived mediators. Read More

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http://dx.doi.org/10.1016/j.jcmgh.2019.02.010DOI Listing

Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro.

Cell Mol Gastroenterol Hepatol 2019 Mar 13. Epub 2019 Mar 13.

Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India. Electronic address:

Background And Aims: Non-dividing hepatocytes in end-stage liver disease indicates permanent growth arrest similar to senescence. Identifying senescence in vivo is often challenging and mechanisms inhibiting senescence are poorly understood. In lower organisms mitochondrial unfolded protein response (UPR) helps in increasing longevity; however, its role in senescence and liver disease is poorly understood. Read More

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http://dx.doi.org/10.1016/j.jcmgh.2019.03.001DOI Listing

Exploration of TiO nanoparticle mediated microdynamic therapy on Cancer treatment.

Nanomedicine 2019 Mar 13. Epub 2019 Mar 13.

Department of Orthopedics, Guangdong General Hospital, Guangdong, Academy of Medical Sciences, Guangzhou, China. Electronic address:

Radical therapy takes advantage of the reactive oxygen species produced in greater quantities within tumor cells than in normal cells. Here, for the first time, we explore a TiO nanoparticle mediated microwave induced radical therapy (termed as Microdynamic Therapy) as a new cancer treatment method. The experiments in vitro and in vivo demonstrate that colloidal TiO nanoparticles could significantly suppress the growth of osteosarcomas, even under low power (5W) microwave (MW) irradiation for 5min. Read More

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http://dx.doi.org/10.1016/j.nano.2019.02.016DOI Listing

Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo.

Free Radic Biol Med 2019 Mar 13. Epub 2019 Mar 13.

Division of Nephrology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address:

Traffic emission is responsible for most small-sized particulate matter (PM) air pollution in urban areas. Several recent studies have indicated that traffic-related PM may aggravate kidney disease. Furthermore, exposure to particulate air pollution may be related to the risk of chronic kidney disease (CKD). Read More

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http://dx.doi.org/10.1016/j.freeradbiomed.2019.03.008DOI Listing

Simultaneous blocking of CD47 and PD-L1 increases innate and adaptive cancer immune responses and cytokine release.

EBioMedicine 2019 Mar 14. Epub 2019 Mar 14.

Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou, China; Marine Drug R&D Center, Institute of Oceangraphy, Minjiang University, Fuzhou 350108, China. Electronic address:

Background: Treatment multiple tumors by immune therapy can be achieved by mobilizing both innate and adaptive immunity. The programmed death ligand 1 (PD-L1; or CD274, B7-H1) is a critical "don't find me" signal to the adaptive immune system. Equally CD47 is a critical "don't eat me" signal to the innate immune system and a regulator of the adaptive immune response. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S23523964193015
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http://dx.doi.org/10.1016/j.ebiom.2019.03.018DOI Listing
March 2019
3 Reads

A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.

Int J Pharm 2019 Mar 12. Epub 2019 Mar 12.

GDHPPCLab, School of Chemistry, Sun Yat-Sen University, 510275, China; Key Lab for Polymer Composite and Functional Materials of Ministry of Education, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address:

FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery. The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy. Spherical FK506 / NH-PEG-b-PLA / HPMC NMs with mean diameter of 101. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.03.022DOI Listing

F-actin dynamics regulates mammalian organ growth and cell fate maintenance.

J Hepatol 2019 Mar 13. Epub 2019 Mar 13.

Department of Molecular Medicine DMM, University of Padova, Italy. Electronic address:

Background & Aims: In vitro, several data indicate that cell function can be regulated by the mechanical properties of cells and of the microenvironment. Cells measure these features by developing forces via their actomyosin cytoskeleton, and respond accordingly by transducing forces into biochemical signals that instruct cell behavior. Among these, the transcriptional coactivators YAP/TAZ recently emerged as key factors mediating multiple responses to actomyosin contractility. Read More

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http://dx.doi.org/10.1016/j.jhep.2019.02.022DOI Listing

Seno-suppressive molecules as new therapeutic perspectives in rheumatic diseases.

Biochem Pharmacol 2019 Mar 13. Epub 2019 Mar 13.

IRMB (Institut of Regenerative Medicine and Biotherapies), Inserm U1183, Univ Montpellier, Montpellier, France. Electronic address:

Over the past years, through in vitro studies and unique animal models, biologists and clinicians have demonstrated that cellular senescence is at the root of numerous age-related chronic diseases including osteoarthritis and osteoporosis. This non-proliferative cellular syndrome can modify other surrounding tissue-resident cells through the establishment of a deleterious catabolic and inflammatory microenvironment. Targeting these deleterious cells through local or systemic seno-therapeutic agent delivery in pre-clinical models improves dramatically clinical signs and extends health span. Read More

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http://dx.doi.org/10.1016/j.bcp.2019.03.017DOI Listing

Korean Red ginseng extract inhibits glioblastoma propagation by blocking the Wnt signaling pathway.

J Ethnopharmacol 2019 Mar 13. Epub 2019 Mar 13.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea; Institute of Animal Molecular Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

Ethnopharmacological Relevance: Korean Red ginseng extract (RG) is one of the most widely used traditional health functional food in Asia, which invigorates immunity and vital energy. RG have been suggested to inhibit proliferation, invasion, and inflammation in several cancer cell lines. Correspondingly, clinical studies have raised the possibility that RG could augment therapeutic efficacy in cancer patients. Read More

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http://dx.doi.org/10.1016/j.jep.2019.03.031DOI Listing

Glycerol derived process contaminants in refined coconut oil induce cholesterol synthesis in HepG2 cells.

Food Chem Toxicol 2019 Mar 13. Epub 2019 Mar 13.

Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA; Dell Pediatric Research Institute and the Depts. of Chemistry, of Pediatrics, and of Nutrition, University of Texas at Austin, 1400 Barbara Jordan Blvd, Austin, TX, 78723, USA. Electronic address:

Despite its 50-year history, the conventional diet-heart hypothesis holding that dietary saturated fats raise serum cholesterol, and with it, cardiovascular risk, remains controversial. Harsh chemical and physical treatment generates process contaminants, and refined oils raise serum and tissue cholesterol in vivo independent of saturated fat content. We developed an in vitro bioassay for rapidly assessing the influence of oils on cholesterol metabolism in the human liver HepG2 cell line, and tested it using coconut oil (CO) of various stages of refinement. Read More

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http://dx.doi.org/10.1016/j.fct.2019.03.005DOI Listing

The γ-secretase inhibitor GSI-I interacts synergistically with the proteasome inhibitor bortezomib to induce ALK+ anaplastic large cell lymphoma cell apoptosis.

Cell Signal 2019 Mar 13. Epub 2019 Mar 13.

Department of Hematology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China. Electronic address:

Single agent treatment of the γ-secretase inhibitor (GSI-I) or proteasome inhibitor in anaplastic lymphoma kinase positive anaplastic large cell lymphoma (ALK+ ALCL) shows limited response and considerable toxicity. Here, we examined the effects of the combination of low dose GSI-I and the proteasome inhibitor bortezomib (BTZ) in ALK+ ALCL cells in vivo and in vitro. We found that ALK+ ALCL cells treated with the BTZ and GSI-I combination treatment showed elevated apoptosis, consistent with increased caspase activation, compared with BTZ or GSI-I alone. Read More

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http://dx.doi.org/10.1016/j.cellsig.2019.03.013DOI Listing

Size-Adjustable Micelles Co-loaded with Chemotherapeutic Agent and Autophagy Inhibitor for Enhancing Cancer Treatment via Increasing Tumor Retention.

Acta Biomater 2019 Mar 13. Epub 2019 Mar 13.

Key Laboratory of Drug Targeting and Drug Delivery Systems, West China School of Pharmacy, China. Electronic address:

Autophagy plays a key role in the stress response of tumor cells, which contributes to cancer cell survival and resistance to chemotherapy by degrading cytoplasmic proteins to provide energy and clear the hazardous substances. Therefore, combined treatment of chemotherapeutics and autophagy inhibitors is thought to obtain a desirable antitumor effect. Nanoparticles (NPs) show potential in tumor-targeting drug delivery because of the enhanced permeability and retention (EPR) effect. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.03.022DOI Listing

MiR-155-3p acts as a tumor suppressor and reverses paclitaxel resistance via negative regulation of MYD88 in human breast cancer.

Gene 2019 Mar 13. Epub 2019 Mar 13.

Department of Biochemistry and Molecular Biology, Bengbu Medical College, Anhui 233030, China. Electronic address:

MiR-155-3p, which is derived from the same pre-miRNA as miR-155-5p, the latter has been reported to be dysregulated in multiple tumor tissues and associated with clinicopathologic markers, tumor subtypes, and poor survival rates. However, the biological effects of miR-155-3p are rarely explored. In this study, we find that miR-155-3p was down-regulated in breast cancer and MYD88 was validated as the target for miR-155-3p. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.066DOI Listing

The germline-specific expression of Foxl3a and its paralogous Foxl3b are associated with male gonadal differentiation in the Japanese eel, Anguilla japonica.

Gen Comp Endocrinol 2019 Mar 13. Epub 2019 Mar 13.

Department of Aquaculture, National Taiwan Ocean University, Keelung 202, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan. Electronic address:

Unlike its paralog Foxl2, which is well known for its role in ovarian development in vertebrates, the function of Foxl3 is still unclear. Foxl3 is an ancient duplicated copy of Foxl2. It is present as a single copy in ray-finned fish. Read More

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http://dx.doi.org/10.1016/j.ygcen.2019.03.008DOI Listing

Aspirin inhibits hypoxia-mediated lung cancer cell stemness and exosome function.

Pathol Res Pract 2019 Mar 11. Epub 2019 Mar 11.

Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, China. Electronic address:

Background: Epidemiological studies have illustrated that regular aspirin consumption may decrease the risk of non-small cell lung cancer (NSCLC). The present study aims to investigate the mechanism of aspirin-induced inhibition of NSCLC development during hypoxia.

Methods: A549 cells were pre-treated with the vehicle control or aspirin and then subjected to hypoxic culture. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03440338183172
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http://dx.doi.org/10.1016/j.prp.2019.03.008DOI Listing
March 2019
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Engineering a uniaxial substrate-stretching device for simultaneous electrophysiological measurements and imaging of strained peripheral neurons.

Med Eng Phys 2019 Mar 13. Epub 2019 Mar 13.

Institute of Biomedical Engineering, Dept. of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK. Electronic address:

Peripheral nerves are continuously subjected to mechanical strain during everyday movements, but excessive stretch can lead to damage and neuronal cell functionality can also be impaired. To better understand cellular processes triggered by stretch, it is necessary to develop in vitro experimental methods that allow multiple concurrent measurements and replicate in vivo mechanical conditions. Current commercially available cell stretching devices do not allow flexible experimental design, restricting the range of possible multi-physics measurements. Read More

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http://dx.doi.org/10.1016/j.medengphy.2019.02.014DOI Listing

Cordycepin suppresses cell proliferation and migration by targeting CLEC2 in human gastric cancer cells via Akt signaling pathway.

Life Sci 2019 Mar 13. Epub 2019 Mar 13.

Key Laboratory of the Science and Technology Department of Jilin Province, China; Cancer Research Center, Yanbian University, Jilin, Yanbian 133002, China; Key Laboratory of the Changbai Mountain, Yanbian University, Jilin, Yanbian 133002, China. Electronic address:

Purpose: Gastric cancer is a common malignancy worldwide, and is associated with high morbidity and mortality rates. Cordycepin is a 3'-deoxyadenosine drug with significant anti-cancer effects. The aim of this study was to determine the molecular mechanisms underlying cordycepin action on gastric cancer cell proliferation and migration. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.03.025DOI Listing

Matrix metalloproteinase-7 protects against acute kidney injury by priming renal tubules for survival and regeneration.

Kidney Int 2019 Mar 8. Epub 2019 Mar 8.

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA; State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address:

Matrix metalloproteinase-7 (MMP-7) is a secreted endopeptidase that degrades a broad range of substrates. Recent studies have identified MMP-7 as an early biomarker to predict severe acute kidney injury (AKI) and poor outcomes after cardiac surgery; however, the role of MMP-7 in the pathogenesis of AKI is unknown. In this study, we investigated the expression of MMP-7 and the impact of MMP-7 deficiency in several models of AKI. Read More

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http://dx.doi.org/10.1016/j.kint.2018.11.043DOI Listing

Calcimimetics maintain bone turnover in uremic rats despite the concomitant decrease in parathyroid hormone concentration.

Kidney Int 2019 Mar 12. Epub 2019 Mar 12.

Maimonides Institute for Biomedical Research (IMIBIC), Cordoba, Spain; Nephrology Service, Reina Sofia University Hospital, Cordoba, Spain; University of Cordoba, Cordoba, Spain; Spanish Renal Research Network (REDinREN), Institute of Health Carlos III, Madrid, Spain.

Calcimimetics decrease parathyroid hormone (PTH) secretion in patients with secondary hyperparathyroidism. The decrease in PTH should cause a reduction in bone turnover; however, the direct effect of calcimimetics on bone cells, which express the calcium-sensing receptor (CaSR), has not been defined. In this study, we evaluated the direct bone effects of CaSR activation by a calcimimetic (AMG 641) in vitro and in vivo. Read More

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http://dx.doi.org/10.1016/j.kint.2018.12.015DOI Listing

Botulinum Toxin Type A Attenuates Apoptosis in Human Dermal Microvascular Endothelial Cells Exposed to an In Vitro Model of Ischemia/Reperfusion Injury.

Transplant Proc 2019 Jan 7. Epub 2019 Jan 7.

Department of Aesthetic Plastic Surgery and Laser Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Background: Botulinum toxin type A (BTXA) has been reported to increase survival of critically ischemic skin flaps; however, the effect of BTXA in human dermal microvascular endothelial cells (HDMECs) remains to be investigated. This study aimed to investigate the protective effect of BTXA in HDMECs exposed to an in vitro model of ischemia/reperfusion injury.

Methods: HDMECs were isolated from human upper eyelid tissue and were randomly divided into 3 groups: 1. Read More

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http://dx.doi.org/10.1016/j.transproceed.2018.11.006DOI Listing
January 2019

Hyaluronan Disrupts Cardiomyocyte Organization within 3D Fibrin-Based Hydrogels.

Biophys J 2019 Feb 26. Epub 2019 Feb 26.

Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania.

The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function. Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction. Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix. Read More

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http://dx.doi.org/10.1016/j.bpj.2019.02.018DOI Listing
February 2019

Structure-based identification of novel CK2 inhibitors with a linear 2-propenone scaffold as anti-cancer agents.

Biochem Biophys Res Commun 2019 Mar 13. Epub 2019 Mar 13.

Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China.

Protein kinase CK2 has emerged as an attractive cancer therapeutic target. Previous studies have highlighted the challenge of optimizing CK2 ATP-competitive inhibitors that have low druggability due to their polycyclic ring scaffolds. Therefore the development of novel inhibitors with non-polycyclic scaffolds emerges as a promising strategy for drug discovery targeting CK2. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.016DOI Listing

Acaricidal activity and enzyme inhibitory activity of active compounds of essential oils against Psoroptes cuniculi.

Vet Parasitol 2019 Mar 12;267:54-59. Epub 2019 Feb 12.

School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China.

Plant essential oils and its chemical compositions are commonly applied in medicinal and other industries due to their broad advanced pharmacological activities. In the present study, we systematically evaluated the acaricidal activities of twelve compounds of essential oils against Psoroptes cuniculi in vitro and in vivo. In addition, to support the clinic uses, their toxicities against immortalized human keratinocytes (HaCaT) and human liver cells (HL-7702) and skin irritation were studied for evaluating the liver and skin safety. Read More

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http://dx.doi.org/10.1016/j.vetpar.2019.01.013DOI Listing

The effects of cooking oil fumes-derived PM on blood vessel formation through ROS-mediated NLRP3 inflammasome pathway in human umbilical vein endothelial cells.

Ecotoxicol Environ Saf 2019 Mar 13;174:690-698. Epub 2019 Mar 13.

Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address:

Background: Cooking oil fumes (COFs), a main pollutant in kitchen air, is a major risk to human health. In our previous research, exposure to COFs-derived PM could cause umbilical vascular endothelial dysfunction, leading to decreased fetal weight. Here, to test the role of ROS-mediated NLRP3 inflammasome pathway in blood vessel formation of human umbilical vein endothelial cells (HUVECs) caused by COFs-derived PM, the cells were exposed to COFs-derived PM at different concentrations with and without N-acetyl-L-cysteine (NAC). Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.03.028DOI Listing
March 2019
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Design, synthesis and evaluation of novel diaryl-1,5-diazoles derivatives bearing morpholine as potent dual COX-2/5-LOX inhibitors and antitumor agents.

Eur J Med Chem 2019 Mar 8;169:168-184. Epub 2019 Mar 8.

State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210023, PR China. Electronic address:

In this paper, 41 hybrid compounds containing diaryl-1,5-diazole and morpholine structures acting as dual COX-2/5-LOX inhibitors have been designed, synthesized and biologically evaluated. Most of them showed potent antiproliferative activities and COX-2/5-LOX inhibitory in vitro. Among them, compound A33 displayed the most potency against cancer cell lines (IC = 6. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.03.008DOI Listing

A peptide antagonist of F11R/JAM-A reduces plaque formation and prolongs survival in an animal model of atherosclerosis.

Atherosclerosis 2019 Feb 22;284:92-101. Epub 2019 Feb 22.

Department of Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY, 11203, USA.

Background And Aims: The F11 Receptor (F11R), AKA Junctional Adhesion Molecule-A (JAM-A) (F11R/JAM-A), is an adhesion protein constitutively expressed on the membrane surface of circulating platelets and the luminal surface of inflamed endothelial cells (EC). Platelet adhesion to an inflamed endothelium is one of the early steps of atherosclerotic plaque formation. Our previous studies, conducted with cultured EC in vitro, have demonstrated the expression of F11R/JAM-A on the luminal surface of inflamed EC, platelet adhesion to inflamed EC through F11R/JAM-A interactions, and inhibition of this interaction by the presence of F11R/JAM-A antagonistic peptide (F11Rpeptide 4D). Read More

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http://dx.doi.org/10.1016/j.atherosclerosis.2019.02.014DOI Listing
February 2019

Anti-biofilm, nitric oxide inhibition and wound healing potential of purpurin-18 phytyl ester isolated from Clinacanthus nutans leaves.

Biomed Pharmacother 2019 Mar 13;113:108724. Epub 2019 Mar 13.

Faculty of Dentistry, Thammasat University, (Rangsit Campus), Paholyothin Road, Klongluang, Pathumthani 12121, Thailand; Center of Excellence in Medicinal Herbs for Treatment of Oral Diseases, Thammasat University. Electronic address:

Aims: Clinacanthus nutans (C. nutans) has demonstrated anti-inflammatory activity, however, the active compound generating this activity remains unknown. The aim of this study was to identify the bioactive compound in C. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108724DOI Listing

Brain-derived neurotrophic factor (BDNF) is expressed in buffalo (Bubalus bubalis) ovarian follicles and promotes oocyte maturation and early embryonic development.

Theriogenology 2019 Feb 23;130:79-88. Epub 2019 Feb 23.

Animal Reproduction Institute, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005, China. Electronic address:

Brain-derived neurotrophic factor (BDNF) has been discovered and characterized for several decades, yet its expression pattern in non-neuronal tissues like ovary and potential mechanism during oocyte maturation are still poorly understood. Thus the present study was devised to determine the expression pattern and mechanism of BDNF during buffalo oocyte maturation. The results revealed that BDNF was presented at different stages of buffalo ovarian follicles as well as during oocyte maturation and early embryo development. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2019.02.020DOI Listing
February 2019

Site-specific conjugation of recognition tags to trastuzumab for peptide nucleic acid-mediated radionuclide HER2 pretargeting.

Biomaterials 2019 Feb 14;203:73-85. Epub 2019 Feb 14.

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Pretargeting is a promising strategy to reach high imaging contrast in a shorter time than by targeting with directly radiolabeled monoclonal antibodies (mAbs). One of problems in pretargeting is a site-specific, reproducible and uniform conjugation of recognition tags to mAbs. To solve this issue we propose a photoconjugation to covalently couple a recognition tag to a mAb via a photoactivatable Z domain. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.02.012DOI Listing
February 2019

[Bioinks and in vitro neurovascular unit production - New prospects in Alzheimer's disease research].

Hell J Nucl Med 2019 Jan-Apr;22 Suppl:209-222

Laboratory of Biochemistry, Dept. of Chemistry, Aristotle University, 54124, Thessaloniki, Greece.

Neurovascular dysfunction is a central process in the pathogenesis of the stroke and most neurodegenerative diseases, including Alzheimer's disease. The multi-cell neurovascular unit (NVU) combines the components of the neural, vascular and extracellular matrix (ECM) into an important interface whose proper function is critical to maintaining brain health. Tissue engineering now offers new tools and information to promote understanding of NVU's operation. Read More

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January 2019

Mukonal Inhibits Cell Proliferation, Alters Mitochondrial Membrane Potential and Induces Apoptosis and Autophagy in Human CNE1 Nasopharyngeal Carcinoma Cells.

Med Sci Monit 2019 Mar 16;25:1976-1983. Epub 2019 Mar 16.

Department of Otolaryngology, The Second Hospital of Jilin University, Changchun, Jilin, China (mainland).

BACKGROUND Nasopharyngeal carcinoma results in high patient morbidity and mortality, due to early metastasis, and toxicity due to chemotherapy. Mukonal is plant-derived carbazole alkaloid that has been used in traditional Chinese medicine to treat several types of cancer. This study aimed to investigate the effects of mukonal on cell proliferation, apoptosis, autophagy, and the mitochondrial membrane potential of nasopharyngeal carcinoma cells in vitro. Read More

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http://dx.doi.org/10.12659/MSM.913915DOI Listing

Gemigliptin Attenuates Renal Fibrosis Through Down-Regulation of the NLRP3 Inflammasome.

Diabetes Metab J 2019 Mar 5. Epub 2019 Mar 5.

Division of Endocrinology and Metabolism, Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Korea.

Background: The hypoglycemic drugs dipeptidyl peptidase-4 (DPP-4) inhibitors have proven protective effects on diabetic kidney disease, including renal fibrosis. Although NOD-like receptor protein 3 (NLRP3) inflammasome activation is known to play an important role in the progression of renal fibrosis, the impact of DPP-4 inhibition on NLRP3-mediated inflammation while ameliorating renal fibrosis has not been fully elucidated. Here, we report that the renoprotective effect of gemigliptin is associated with a reduction in NLRP3-mediated inflammation in a murine model of renal fibrosis. Read More

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http://dx.doi.org/10.4093/dmj.2018.0181DOI Listing

In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells.

Methods Mol Biol 2019 ;1969:135-148

Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany.

Bacterial meningitis is a serious, life-threatening infection of the central nervous system (CNS). To cause meningitis, bacteria must interact with and penetrate the meningeal blood-cerebrospinal fluid barrier (mB/CSFB), which comprises highly specialized brain endothelial cells. Neisseria meningitidis (meningococcus) is a leading cause of bacterial meningitis, and examination meningococcus' interaction with the BBB is critical for understanding disease progression. Read More

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http://dx.doi.org/10.1007/978-1-4939-9202-7_10DOI Listing
January 2019

Aflatoxin production and in vitro toxicity of Aspergilli section Flavi isolated from air samples collected from different environments.

Mycotoxin Res 2019 Mar 12. Epub 2019 Mar 12.

Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, 10000, Zagreb, Croatia.

Aspergilli section Flavi, originally isolated from air samples collected from inhabited apartments (AP), unoccupied basements (BS), and processing facilities of a grain mill (GM), were analyzed for their potential to produce aflatoxin B (AFB) on solid media. The isolates were further characterized with regard to their cytotoxic, genotoxic, and pro-inflammatory properties in vitro. Aspergilli were identified based on partial calmodulin (CaM) gene sequencing; the producing capacities of isolates were analyzed by HPLC/FLD and confirmed by genes in biosynthesis (aflR, norA, omtA). Read More

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http://dx.doi.org/10.1007/s12550-019-00345-zDOI Listing

Semaphorin 3F Promotes Transendothelial Migration of Leukocytes in the Inflammatory Response After Survived Cardiac Arrest.

Inflammation 2019 Mar 15. Epub 2019 Mar 15.

Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, Hugstetter Strasse 55, 79106, Freiburg im Breisgau, Germany.

Leukocyte transmigration through the blood vessel wall is a fundamental step of the inflammatory response and requires expression of adhesion molecule PECAM-1. Accumulating evidence implicates that semaphorin (Sema) 3F and its receptor neuropilin (NRP) 2 are central regulators in vascular biology. Herein, we assess the role of Sema3F in leukocyte migration in vitro and in vivo. Read More

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http://dx.doi.org/10.1007/s10753-019-00985-4DOI Listing

Identification of naphthalene carboxylase subunits of the sulfate-reducing culture N47.

Biodegradation 2019 Mar 15. Epub 2019 Mar 15.

Biofilm Centre, Universität Duisburg-Essen, Essen, Germany.

Expanding industrialization and the associated usage and production of mineral oil products has caused a worldwide spread of polycyclic aromatic hydrocarbons. These pollutants accumulate and persist under anoxic conditions but little is known about the biochemical reactions catalyzing their anaerobic degradation. Recently, carboxylation of naphthalene was demonstrated for the sulfate-reducing culture N47. Read More

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http://dx.doi.org/10.1007/s10532-019-09872-zDOI Listing

Fluorescence imaging of meningioma cells with somatostatin receptor ligands: an in vitro study.

Acta Neurochir (Wien) 2019 Mar 15. Epub 2019 Mar 15.

Klinik für Neurochirurgie, Universitätsklinikum des Saarlandes, 66421, Homburg, Saar, Germany.

Background: The use of five-aminolevulinic acid (5-ALA) in the staining of malignant glioma cells has significantly improved intraoperative radicality in the resection of gliomas in the last decade. Currently, there is no comparable selective fluorescent substance available for meningiomas. There is however a demand for intraoperative fluorescent identification of, e. Read More

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http://dx.doi.org/10.1007/s00701-019-03872-xDOI Listing

Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.

Acta Neuropathol 2019 Mar 15. Epub 2019 Mar 15.

Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, 10900 Euclid Ave, E516, Cleveland, OH, 44106-4970, USA.

Both α-Synuclein (αSyn) accumulation and mitochondrial dysfunction have been implicated in the pathology of Parkinson's disease (PD). Although studies suggest that αSyn and its missense mutant, A53T, preferentially accumulate in the mitochondria, the mechanisms by which αSyn and mitochondrial proteins regulate each other to trigger mitochondrial and neuronal toxicity are poorly understood. ATP-dependent Clp protease (ClpP), a mitochondrial matrix protease, plays an important role in regulating mitochondrial protein turnover and bioenergetics activity. Read More

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http://dx.doi.org/10.1007/s00401-019-01993-2DOI Listing
March 2019
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Cyclolinopeptide F, a cyclic peptide from flaxseed inhibited RANKL-induced osteoclastogenesis via downergulation of RANK expression.

J Nat Med 2019 Mar 15. Epub 2019 Mar 15.

Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41 Shinagawa-ku, Tokyo, 142-8501, Japan.

Previously, we reported that cyclolinopeptides (CLs) extracted from flaxseed inhibited receptor activator of nuclear factor κ-B ligand (RANKL)-induced osteoclastogenesis from mouse bone marrow cells in vitro. However, mode of action involved in CLs-inhibited osteoclastogenesis has been yet unknown. Therefore, in this study, we investigated the details of inhibitory activity of cyclolinopeptide-F (CL-F) in osteoclastogenesis, as a representative of CLs. Read More

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http://dx.doi.org/10.1007/s11418-019-01292-wDOI Listing

Cancer targeting peptides.

Cell Mol Life Sci 2019 Mar 15. Epub 2019 Mar 15.

Biology Program, New York University Abu Dhabi, PO Box 129188, Saadiyat Island Campus, Abu Dhabi, United Arab Emirates.

Despite continuing advances in the development of biomacromolecules for therapeutic purposes, successful application of these often large and hydrophilic molecules has been hindered by their inability to efficiently traverse the cellular plasma membrane. In recent years, cell-penetrating peptides (CPPs) have received considerable attention as a promising class of delivery vectors due to their ability to mediate the efficient import of a large number of cargoes in vitro and in vivo. However, the lack of target specificity of CPPs remains a major obstacle to their clinical development. Read More

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http://dx.doi.org/10.1007/s00018-019-03061-0DOI Listing
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