216 results match your criteria Immunome Research [Journal]


Maternal Inflammation and Neurodevelopmental Programming: A Review of Preclinical Outcomes and Implications for Translational Psychiatry.

Biol Psychiatry 2018 Aug 27. Epub 2018 Aug 27.

Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, Iowa; Department of Psychiatry, Carver College of Medicine, University of Iowa, Iowa City, Iowa; Iowa Neuroscience Institute, University of Iowa, Iowa City, Iowa. Electronic address:

Early disruptions to neurodevelopment are highly relevant to understanding both psychiatric risk and underlying pathophysiology that can be targeted by new treatments. Much convergent evidence from the human literature associates inflammation during pregnancy with later neuropsychiatric disorders in offspring. Preclinical models of prenatal inflammation have been developed to examine the causal maternal physiological and offspring neural mechanisms underlying these findings. Read More

View Article
August 2018
6 Reads

The immunome of soy bean allergy: Comprehensive identification and characterization of epitopes.

Clin Exp Allergy 2018 Sep 29. Epub 2018 Sep 29.

Ligand Development Unit, Fraunhofer IZI, Leipzig, Germany.

Background: The precise mapping of multiple antibody epitopes recognized by patients' sera allows a more detailed and differentiated understanding of immunological diseases. It may lead to the development of novel therapies and diagnostic tools.

Objective: Mapping soy bean specific epitopes relevant for soy bean allergy patients and persons sensitized to soy bean, and analysis of their IgE/IgG binding spectrum. Read More

View Article
September 2018
1 Read

Immune-Phenotyping and Transcriptomic Profiling of Peripheral Blood Mononuclear Cells From Patients With Breast Cancer: Identification of a 3 Gene Signature Which Predicts Relapse of Triple Negative Breast Cancer.

Front Immunol 2018 11;9:2028. Epub 2018 Sep 11.

John van Geest Cancer Research Centre, School of Science and Technology, Nottingham Trent University, Nottingham, United Kingdom.

Interactions between the immune system and tumors are highly reciprocal in nature, leading to speculation that tumor recurrence or therapeutic resistance could be influenced or predicted by immune events that manifest locally, but can be detected systemically. Multi-parameter flow cytometry was used to examine the percentage and phenotype of natural killer (NK) cells, myeloid-derived suppressor cells (MDSCs), monocyte subsets and regulatory T (Treg) cells in the peripheral blood of of 85 patients with breast cancer (50 of whom were assessed before and after one cycle of anthracycline-based chemotherapy), and 23 controls. Transcriptomic profiles of peripheral blood mononuclear cells (PBMCs) in 23 patients were generated using a NanoString gene profiling platform. Read More

View Article
September 2018
7 Reads

TSNAdb: A Database for Tumor-specific Neoantigens from Immunogenomics Data Analysis.

Genomics Proteomics Bioinformatics 2018 08 15;16(4):276-282. Epub 2018 Sep 15.

Institute of Drug Metabolism and Pharmaceutical Analysis and Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:

Tumor-specific neoantigens have attracted much attention since they can be used as biomarkers to predict therapeutic effects of immune checkpoint blockade therapy and as potential targets for cancer immunotherapy. In this study, we developed a comprehensive tumor-specific neoantigen database (TSNAdb v1.0), based on pan-cancer immunogenomic analyses of somatic mutation data and human leukocyte antigen (HLA) allele information for 16 tumor types with 7748 tumor samples from The Cancer Genome Atlas (TCGA) and The Cancer Immunome Atlas (TCIA). Read More

View Article

Hierarchy of human IgG recognition within the Staphylococcus aureus immunome.

Sci Rep 2018 Sep 5;8(1):13296. Epub 2018 Sep 5.

New York University School of Medicine, Department of Medicine, New York, 10016, USA.

Staphylococcus aureus is an opportunistic pathogen that causes a range of serious infections associated with significant morbidity, by strains increasingly resistant to antibiotics. However, to date all candidate vaccines have failed to induce protective immune responses in humans. We need a more comprehensive understanding of the antigenic targets important in the context of human infection. Read More

View Article
September 2018
28 Reads

Molecular predictors of prevention of recurrence in HCC with sorafenib as adjuvant treatment and prognostic factors in the phase 3 STORM trial.

Gut 2018 Aug 14. Epub 2018 Aug 14.

BCLC Group, Liver Unit, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)-Hospital Clínic, CIBERehd, Universitat de Barcelona, Barcelona, Spain.

Objective: Sorafenib is the standard systemic therapy for advanced hepatocellular carcinoma (HCC). Survival benefits of resection/local ablation for early HCC are compromised by 70% 5-year recurrence rates. The phase 3 STORM trial comparing sorafenib with placebo as adjuvant treatment did not achieve its primary endpoint of improving recurrence-free survival (RFS). Read More

View Article
August 2018
5 Reads

Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies.

MAbs 2018 Oct 1;10(7):1060-1072. Epub 2018 Aug 1.

a Department of Molecular Medicine and Medical Biotechnology , University of Naples "Federico II" , Napoli ( NA ), Italy.

Immune checkpoints are emerging as novel targets for cancer therapy, and antibodies against them have shown remarkable clinical efficacy with potential for combination treatments to achieve high therapeutic index. This work aims at providing a novel approach for the generation of several novel human immunomodulatory antibodies capable of binding their targets in their native conformation and useful for therapeutic applications. We performed a massive parallel screening of phage libraries by using for the first time activated human lymphocytes to generate large collections of single-chain variable fragments (scFvs) against 10 different immune checkpoints: LAG-3, PD-L1, PD-1, TIM3, BTLA, TIGIT, OX40, 4-1BB, CD27 and ICOS. Read More

View Article
October 2018
5 Reads

Immunome differences between porcine ileal and jejunal Peyer's patches revealed by global transcriptome sequencing of gut-associated lymphoid tissues.

Sci Rep 2018 Jun 13;8(1):9077. Epub 2018 Jun 13.

GABI, INRA, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.

The epithelium of the intestinal mucosa and the gut-associated lymphoid tissues (GALT) constitute an essential physical and immunological barrier against pathogens. In order to study the specificities of the GALT transcriptome in pigs, we compared the transcriptome profiles of jejunal and ileal Peyer's patches (PPs), mesenteric lymph nodes (MLNs) and peripheral blood (PB) of four male piglets by RNA-Seq. We identified 1,103 differentially expressed (DE) genes between ileal PPs (IPPs) and jejunal PPs (JPPs), and six times more DE genes between PPs and MLNs. Read More

View Article
June 2018
1 Read

Cellular conditions of weakly chelated magnesium ions strongly promote RNA stability and catalysis.

Nat Commun 2018 06 1;9(1):2149. Epub 2018 Jun 1.

Department of Chemistry, Pennsylvania State University, University Park, PA, 16802, USA.

Most RNA folding studies have been performed under non-physiological conditions of high concentrations (≥10 mM) of Mg, while actual cellular concentrations of Mg are only ~1 mM in a background of greater than 50 mM Mg. To uncover cellular behavior of RNA, we devised cytoplasm mimic systems that include biological concentrations of amino acids, which weakly chelate Mg. Amino acid-chelated Mg (aaCM) of ~15 mM dramatically increases RNA folding and prevents RNA degradation. Read More

View Article
June 2018
1 Read

The Human Vaccines Project: Towards a comprehensive understanding of the human immune response to immunization.

Hum Vaccin Immunother 2018 28;14(9):2214-2216. Epub 2018 Jun 28.

b Human Vaccines Project , NY , NY , USA.

Although the success of vaccination to date has been unprecedented, our inadequate understanding of the details of the human immune response to immunization has resulted in several recent vaccine failures and significant delays in the development of high-need vaccines for global infectious diseases and cancer. Because of the need to better understand the immense complexity of the human immune system, the Human Vaccines Project was launched in 2015 with the mission to decode the human immune response to accelerate development of vaccines and immunotherapies for major diseases. The Project currently has three programs: 1) The Human Immunome Program, with the goal of deciphering the complete repertoire of B and T cell receptors across the human population, termed the Human Immunome, 2) The Rules of Immunogenicity Program, with the goal of understanding the key principles of how a vaccine elicits a protective and durable response using a system immunology approach, and 3) The Universal Influenza Vaccine Initiative (UIVI), with the goal of conducting experimental clinical trials to understand the influence of influenza pre-exposures on subsequent influenza immunization and the mechanisms of protection. Read More

View Article
June 2018
1 Read

Multi-omics in high-grade serous ovarian cancer: Biomarkers from genome to the immunome.

Am J Reprod Immunol 2018 Aug 21;80(2):e12975. Epub 2018 May 21.

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.

Epithelial ovarian cancer (EOC) is a lethal gynaecological disease that imposes significant burden on health care and patient quality of life. High-grade serous carcinoma of the ovary (HGSC) is the most prevalent histological type of EOC. A vast majority of HGSC cases are diagnosed at late stages of the disease, limiting the opportunity for clinical intervention and resulting in a 10-year survival rate of <20%. Read More

View Article
August 2018
2 Reads

Macrophages induce "budding" in aggressive human colon cancer subtypes by protease-mediated disruption of tight junctions.

Oncotarget 2018 Apr 13;9(28):19490-19507. Epub 2018 Apr 13.

UMC Utrecht Cancer Center, University Medical Center Utrecht, Utrecht, The Netherlands.

Primary human colorectal tumors with a high stromal content have an increased capacity to metastasize. Cancer-associated fibroblasts (CAFs) promote metastasis, but the contribution of other stromal cell types is unclear. Here we searched for additional stromal cell types that contribute to aggressive tumor cell behavior. Read More

View Article
April 2018
10 Reads

Integrative network analyses of wilt transcriptome in chickpea reveal genotype dependent regulatory hubs in immunity and susceptibility.

Sci Rep 2018 Apr 25;8(1):6528. Epub 2018 Apr 25.

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

Host specific resistance and non-host resistance are two plant immune responses to counter pathogen invasion. Gene network organizing principles leading to quantitative differences in resistant and susceptible host during host specific resistance are poorly understood. Vascular wilt caused by root pathogen Fusarium species is complex and governed by host specific resistance in crop plants, including chickpea. Read More

View Article
April 2018
3 Reads

Immune Control of --Common, High-Frequency T-Cell Responses to a Broad Repertoire of Immunoprevalent Epitopes.

Front Immunol 2018 20;9:484. Epub 2018 Mar 20.

Centre for Research & Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.

(Bp) is an environmental bacterial pathogen that causes potentially lethal sepsis in susceptible individuals and is considered a Category B, Tier-1 biothreat agent. As such, it is crucial to gain an improved understanding of protective immunity and potential vaccine candidates. The nature of immune correlates dictating why most exposed individuals in endemic regions undergo asymptomatic seroconversion while others succumb to life-threatening sepsis is largely uncharted. Read More

View Article
March 2018
13 Reads
1 Citation

The Immunome of Colon Cancer: Functional In Silico Analysis of Antigenic Proteins Deduced from IgG Microarray Profiling.

Genomics Proteomics Bioinformatics 2018 02 2;16(1):73-84. Epub 2018 Mar 2.

Molecular Diagnostics, AIT - Austrian Institute of Technology, A-1190 Vienna, Austria. Electronic address:

Characterization of the colon cancer immunome and its autoantibody signature from differentially-reactive antigens (DIRAGs) could provide insights into aberrant cellular mechanisms or enriched networks associated with diseases. The purpose of this study was to characterize the antibody profile of plasma samples from 32 colorectal cancer (CRC) patients and 32 controls using proteins isolated from 15,417 human cDNA expression clones on microarrays. 671 unique DIRAGs were identified and 632 were more highly reactive in CRC samples. Read More

View Article
February 2018
7 Reads

The Immunome in Two Inherited Forms of Pulmonary Fibrosis.

Front Immunol 2018 31;9:76. Epub 2018 Jan 31.

Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, United States.

The immunome (immune cell phenotype, gene expression, and serum cytokines profiling) in pulmonary fibrosis is incompletely defined. Studies focusing on inherited forms of pulmonary fibrosis provide insights into mechanisms of fibrotic lung disease in general. To define the cellular and molecular immunologic phenotype in peripheral blood, high-dimensional flow cytometry and large-scale gene expression of peripheral blood mononuclear cells and serum proteomic multiplex analyses were performed and compared in a cohort with familial pulmonary fibrosis (FPF), an autosomal dominant disorder with incomplete penetrance; Hermansky-Pudlak syndrome pulmonary fibrosis (HPSPF), a rare autosomal recessive disorder; and their unaffected relatives. Read More

View Article
January 2018
5 Reads

Identifying protein biomarkers in predicting disease severity of dengue virus infection using immune-related protein microarray.

Medicine (Baltimore) 2018 Feb;97(5):e9713

Faculty of Medicine, MAHSA University, Bandar Saujana Putra.

Dengue virus is one of the most widespread flaviviruses that re-emerged throughout recent decades. The progression from mild dengue to severe dengue (SD) with the complications such as vascular leakage and hemorrhage increases the fatality rate of dengue. The pathophysiology of SD is not entirely clear. Read More

View Article
February 2018
3 Reads

Profiling the immunome of little brown myotis provides a yardstick for measuring the genetic response to white-nose syndrome.

Evol Appl 2017 12 3;10(10):1076-1090. Epub 2017 Sep 3.

Forensic Science Department Trent University Peterborough ON Canada.

White-nose syndrome (WNS) has devastated populations of hibernating bats in eastern North America, leading to emergency conservation listings for several species including the previously ubiquitous little brown myotis (). However, some bat populations near the epicenter of the WNS panzootic appear to be stabilizing after initial precipitous declines, which could reflect a selective immunogenetic sweep. To investigate the hypothesis that WNS exerts significant selection on the immunome of affected bat populations, we developed a novel, high-throughput sequence capture assay targeting 138 adaptive, intrinsic, and innate immunity genes of putative adaptive significance, as well as their respective regulatory regions (~370 kbp of genomic sequence/individual). Read More

View Article
December 2017
2 Reads

DNA vaccines for fish: Review and perspectives on correlates of protection.

Authors:
R A Dalmo

J Fish Dis 2018 Jan 24;41(1):1-9. Epub 2017 Oct 24.

Faculty of Biosciences, Fisheries & Economics, Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway.

Recently in 2016, the European Medicines Agency (EMA) recommended granting a marketing authorization in the EU for "Clynav," a DNA vaccine against salmon pancreas disease (salmonid alphavirus-3). Generally, DNA vaccines induce both early and late immune responses in fish that may be protective against disease. Several transcriptomic approaches have been performed to map immunome profiles following DNA vaccination, but the precise immune mechanism(s) that is responsible for protection is not known, although reasonable suggestions have been made. Read More

View Article
January 2018
3 Reads

Computational flow cytometry analysis reveals a unique immune signature of the human maternal-fetal interface.

Am J Reprod Immunol 2018 Jan 14;79(1). Epub 2017 Oct 14.

Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA.

Problem: Decidual immune dysregulation is thought to underlie major pregnancy disorders; however, incomplete understanding of the decidual immune interface has hampered the mechanistic investigation.

Method Of Study: Human term decidua was collected, and single-cell phenotypic information was acquired by highly polychromatic flow cytometry. Cellular identity analysis was performed with t-distributed stochastic neighbor embedding, DensVM clustering, and matched to CellOntology database. Read More

View Article
January 2018
20 Reads

Low antigen dose formulated in CAF09 adjuvant Favours a cytotoxic T-cell response following intraperitoneal immunization in Göttingen minipigs.

Vaccine 2017 10 5;35(42):5629-5636. Epub 2017 Sep 5.

Division of Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. Electronic address:

The relationship between the antigen dose and the quality of an immune response generated upon immunization is poorly understood. However, findings show that the immune system is indeed influenced by the antigen dose; hence underlining the importance of correctly determining which dose to use in order to generate a certain type of immune response. To investigate this area further, we used Göttingen minipigs asan animal model especially due to the similar body size and high degree of immunome similarity between humans and pigs. Read More

View Article
October 2017
7 Reads

The prognostic landscape of tumor-infiltrating immune cell and immunomodulators in lung cancer.

Biomed Pharmacother 2017 Nov 18;95:55-61. Epub 2017 Aug 18.

Department of Oncology, Chest Hospital of Hebei Province, Shijiazhuang 050041, PR China.

Tumor-infiltrating immune cells are closely associated with clinical outcome. However, immunohistochemistry-based analysis of tumor infiltrates can be misleading as the representative marker of an immune subpopulation might be expressed in other cell types. In this study, based on a metagene approach (known as CIBERSORT) and an online databse, The Cancer Immunome Atlas (https://tcia. Read More

View Article
November 2017
42 Reads

Characterization of the peripheral blood transcriptome and adaptive evolution of the MHC I and TLR gene families in the wolf (Canis lupus).

BMC Genomics 2017 08 7;18(1):584. Epub 2017 Aug 7.

Qufu Normal University, Jingxuan Street No. 57, Qufu, Shandong province, China.

Background: The wolf (Canis lupus) is one of the most widely distributed terrestrial mammals, because it is well adapted to various ecological niches and their corresponding pathogen environments. Immunological competence is a crucial factor involved in adapting to a changing environment and fighting pathogen infection in animals. In this study, the peripheral blood transcriptome of wolves was generated via RNA-seq to advance understanding of the wolf immunome, with a special focus on the major histocompatibility complex class I (MHC I) and toll-like receptor (TLR) gene families, which are involved in pathogen recognition and defense. Read More

View Article
August 2017
14 Reads

In-depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide.

Sci Immunol 2016 Jul 23;1(1):aaf6925. Epub 2016 Jun 23.

Division of Hematology and Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Despite remarkable recent achievements of immunotherapy strategies in cancer treatment, clinical responses remain limited to subsets of patients. Predictive markers of disease course and response to immunotherapy are urgently needed. Recent results have revealed the potential predictive value of immune cell phenotype and spatial distribution at the tumor site, prompting the need for multidimensional immunohistochemical analyses of tumor tissues. Read More

View Article
July 2016
16 Reads

Advances in the development of human protein microarrays.

Expert Rev Proteomics 2017 07;14(7):627-641

b Institute of Infectious Disease and Molecular Medicine & Department of Integrative Biomedical Sciences, Faculty of Health Sciences , University of Cape Town , Observatory, South Africa.

Introduction: High-content protein microarrays in principle enable the functional interrogation of the human proteome in a broad range of applications, including biomarker discovery, profiling of immune responses, identification of enzyme substrates, and quantifying protein-small molecule, protein-protein and protein-DNA/RNA interactions. As with other microarrays, the underlying proteomic platforms are under active technological development and a range of different protein microarrays are now commercially available. However, deciphering the differences between these platforms to identify the most suitable protein microarray for the specific research question is not always straightforward. Read More

View Article
July 2017
2 Reads

Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh.

J Infect Dis 2017 07;216(1):125-134

Division of Infectious Diseases, Massachusetts General Hospital.

Background: Cholera is a severe dehydrating illness of humans caused by toxigenic strains of Vibrio cholerae O1 or O139. Identification of immunogenic V. cholerae antigens could lead to a better understanding of protective immunity in human cholera. Read More

View Article
July 2017
44 Reads

Analyses of the peripheral immunome following multiple administrations of avelumab, a human IgG1 anti-PD-L1 monoclonal antibody.

J Immunother Cancer 2017 21;5:20. Epub 2017 Feb 21.

Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Room 8B09, Bethesda, MD USA.

Background: Multiple anti-PD-L1/PD-1 checkpoint monoclonal antibodies (MAb) have shown clear evidence of clinical benefit. All except one have been designed or engineered to omit the possibility to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) as a second potential mode of anti-tumor activity; the reason for this is the concern of lysis of PD-L1 positive immune cells. Avelumab is a fully human IgG1 MAb which has been shown in prior in vitro studies to mediate ADCC versus a range of human tumor cells, and clinical studies have demonstrated anti-tumor activity versus a range of human cancers. Read More

View Article
June 2018
14 Reads

Pan-cancer Immunogenomic Analyses Reveal Genotype-Immunophenotype Relationships and Predictors of Response to Checkpoint Blockade.

Cell Rep 2017 01;18(1):248-262

Biocenter, Division of Bioinformatics, Medical University of Innsbruck, 6020 Innsbruck, Austria. Electronic address:

The Cancer Genome Atlas revealed the genomic landscapes of human cancers. In parallel, immunotherapy is transforming the treatment of advanced cancers. Unfortunately, the majority of patients do not respond to immunotherapy, making the identification of predictive markers and the mechanisms of resistance an area of intense research. Read More

View Article
January 2017
4 Reads

Epigenetic regulation of immune checkpoints: another target for cancer immunotherapy?

Immunotherapy 2017 01;9(1):99-108

Clinical Oncology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Epigenetic changes in oncogenes and tumor-suppressor genes contribute to carcinogenesis. Understanding the epigenetic and genetic components of tumor immune evasion is crucial. Few cancer genetic mutations have been linked to direct correlations with immune evasion. Read More

View Article
January 2017
38 Reads
2.440 Impact Factor

Insights into the innate immunome of actiniarians using a comparative genomic approach.

BMC Genomics 2016 11 2;17(1):850. Epub 2016 Nov 2.

School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, GPO Box 2434, Brisbane, Qld, 4000, Australia.

Background: Innate immune genes tend to be highly conserved in metazoans, even in early divergent lineages such as Cnidaria (jellyfish, corals, hydroids and sea anemones) and Porifera (sponges). However, constant and diverse selection pressures on the immune system have driven the expansion and diversification of different immune gene families in a lineage-specific manner. To investigate how the innate immune system has evolved in a subset of sea anemone species (Order: Actiniaria), we performed a comprehensive and comparative study using 10 newly sequenced transcriptomes, as well as three publically available transcriptomes, to identify the origins, expansions and contractions of candidate and novel immune gene families. Read More

View Article
November 2016
4 Reads

Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays.

J Proteome Res 2017 01 17;16(1):195-203. Epub 2016 Oct 17.

The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.

We performed an unbiased proteome-scale profiling of humoral autoimmunity in recent-onset type 1 diabetes (T1D) patients and nondiabetic controls against ∼10 000 human proteins using a Nucleic Acid Programmable Protein Array (NAPPA) platform, complemented by a knowledge-based selection of proteins from genes enriched in human pancreas. Although the global response was similar between cases and controls, we identified and then validated six specific novel T1D-associated autoantibodies (AAbs) with sensitivities that ranged from 16 to 27% at 95% specificity. These included AAbs against PTPRN2, MLH1, MTIF3, PPIL2, NUP50 (from NAPPA screening), and QRFPR (by targeted ELISA). Read More

View Article
January 2017
21 Reads

Strategies to modulate the immune system in breast cancer: checkpoint inhibitors and beyond.

Ther Adv Med Oncol 2016 Sep 10;8(5):360-74. Epub 2016 Jul 10.

Division of Experimental Therapeutics, Istituto Europeo di Oncologia, Via Ripamonti 435, 20141 Milano, Italy.

Is breast cancer (BC) immunogenic? Many data suggest that it is. Many observations demonstrated the prognostic role of tumor-infiltrating lymphocytes (TILs) in triple negative (TN) and human epidermal growth factor receptor 2 (HER-2)-positive BC. TNBCs are poorly differentiated tumors with high genetic instability and very high heterogeneity. Read More

View Article
September 2016
6 Reads

Cyclophilin and enolase are the most prevalent conidial antigens of Lomentospora prolificans recognized by healthy human salivary IgA and cross-react with Aspergillus fumigatus.

Proteomics Clin Appl 2016 10 9;10(9-10):1058-1067. Epub 2016 Sep 9.

Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.

Purpose: The study of the immunocompetent airways immune response may provide important information to improve the therapeutic efficacy against Lomentospora (Scedosporium) prolificans. So, this study aimed to identify the most prevalent conidial antigens of this multiresistant fungus recognized by healthy human salivary immunoglobulin A, and to study their expression and cross-reactivity with other fungal species.

Experimental Design: Twenty saliva from immunocompetent donors were used to detect and identify the immunoreactive proteins by 2D immunoblotting and LC-MS/MS. Read More

View Article
October 2016
5 Reads

Secretome, surfome and immunome: emerging approaches for the discovery of new vaccine candidates against bacterial infections.

World J Microbiol Biotechnol 2016 Sep 27;32(9):155. Epub 2016 Jul 27.

Amity Institute of Biotechnology, Amity University, Gwalior, Madhya Pradesh, India.

Functional genomics has made possible advanced structure-to-function investigation of pathogens and helped characterize virulence mechanisms. Proteomics has been become a tool for large-scale identification of proteins involved during invasion and infection by the pathogens. Bacterial surface and secreted proteins play key role in the interaction between the bacterial cell and the host environment. Read More

View Article
September 2016

An in-depth comparison of the porcine, murine and human inflammasomes; lessons from the porcine genome and transcriptome.

Vet Microbiol 2017 Apr 4;202:2-15. Epub 2016 Jun 4.

U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics and Immunology Laboratory, Beltsville, MD 20705, USA.

Emerging evidence suggests that swine are a scientifically acceptable intermediate species between rodents and humans to model immune function relevant to humans. The swine genome has recently been sequenced and several preliminary structural and functional analysis of the porcine immunome have been published. Herein we provide an expanded in silico analysis using an improved assembly of the porcine transcriptome that provides an in depth analysis of genes that are related to inflammasomes, responses to Toll-like receptor ligands, and M1 macrophage polarization and Escherichia coli as a model organism. Read More

View Article
April 2017
11 Reads

Augmentation of Autoantibodies by Helicobacter pylori in Parkinson's Disease Patients May Be Linked to Greater Severity.

PLoS One 2016 21;11(4):e0153725. Epub 2016 Apr 21.

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Parkinson's disease (PD) is the second most common chronic and progressive neurodegenerative disorder. Its etiology remains elusive and at present only symptomatic treatments exists. Helicobacter pylori chronically colonizes the gastric mucosa of more than half of the global human population. Read More

View Article
September 2016
11 Reads

The prostate cancer immunome: In silico functional analysis of antigenic proteins from microarray profiling with IgG.

Proteomics 2016 Apr 4;16(8):1204-14. Epub 2016 Apr 4.

Molecular Diagnostics, Health & Environment Department, Austrian Institute of Technology - AIT, Vienna, Austria.

The study of the immunome of prostate cancer (PCa) and characterization of autoantibody signature from differentially reactive antigens can uncover disease stage proteins, reveal enriched networks and even expose aberrant cellular mechanisms during the disease process. By conducting plasma IgG profiling on protein microarrays presenting 5449 unique human proteins expressed in 15 417 E. coli human cDNA expression clones, we elucidated 471 (21 higher reactive in PCa) differentially reactive antigens in 50 PCa versus 49 patients with benign prostate hyperplasia (BPH) at initial diagnosis. Read More

View Article
April 2016
5 Reads

A transplant "immunome" screening platform defines a targetable epitope fingerprint of multiple myeloma.

Blood 2016 06 31;127(25):3202-14. Epub 2016 Mar 31.

Department of Oncology and Hematology, Bone Marrow Transplantation with section Pneumology, Hubertus Wald Tumorzentrum/University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;

Multiple myeloma (MM) is a hematological cancer for which immune-based treatments are currently in development. Many of these rely on the identification of highly disease-specific, strongly and stably expressed antigens. Here, we profiled the myeloma B-cell immunome both to explore its predictive role in the context of autologous and allogeneic hematopoietic stem cell transplantation (HSCT) and to identify novel immunotherapeutic targets. Read More

View Article
June 2016
19 Reads

Tracing the evolutionary lineage of pattern recognition receptor homologues in vertebrates: An insight into reptilian immunity via de novo sequencing of the wall lizard splenic transcriptome.

Vet Immunol Immunopathol 2016 Apr 4;172:26-37. Epub 2016 Mar 4.

Department of Zoology, Hindu College, University of Delhi, Delhi 110007, India. Electronic address:

Reptiles remain a deprived class in the area of genomic and molecular resources for the vertebrate classes. The transition of squamates from aquatic to terrestrial mode of life caused profound changes in their immune system to combat the altered variety of pathogens on land. The current study aims at delineating the evolution of defence mechanisms in wall lizard, Hemidactylus flaviviridis, by exploring its immunome. Read More

View Article
April 2016
1 Read

Decoding the human immune system to transform the future of global disease prevention and control.

Expert Rev Vaccines 2016 10 25;15(10):1235-6. Epub 2016 May 25.

b West Monument Consulting , Washington , DC , USA.

View Article
October 2016
1 Read

Effects of Systemically Administered Hydrocortisone on the Human Immunome.

Sci Rep 2016 Mar 14;6:23002. Epub 2016 Mar 14.

Trans-NIH Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institutes of Health (NIH), Bethesda, MD, 20892, USA.

Corticosteroids have been used for decades to modulate inflammation therapeutically, yet there is a paucity of data on their effects in humans. We examined the changes in cellular and molecular immune system parameters, or "immunome", in healthy humans after systemic corticosteroid administration. We used multiplexed techniques to query the immunome in 20 volunteers at baseline, and after intravenous hydrocortisone (HC) administered at moderate (250 mg) and low (50 mg) doses, to provide insight into how corticosteroids exert their effects. Read More

View Article
March 2016
11 Reads
11 Citations
5.080 Impact Factor

Immunomics: a 21st century approach to vaccine development for complex pathogens.

Parasitology 2016 Feb;143(2):236-44

Infectious Diseases Programme,QIMR Berghofer Medical Research Institute,Herston,QLD 4029,Australia.

Immunomics is a relatively new field of research which integrates the disciplines of immunology, genomics, proteomics, transcriptomics and bioinformatics to characterize the host-pathogen interface. Herein, we discuss how rapid advances in molecular immunology, sophisticated tools and molecular databases are facilitating in-depth exploration of the immunome. In our opinion, an immunomics-based approach presides over traditional antigen and vaccine discovery methods that have proved ineffective for highly complex pathogens such as the causative agents of malaria, tuberculosis and schistosomiasis that have evolved genetic and immunological host-parasite adaptations over time. Read More

View Article
February 2016
6 Reads

Top-down characterization data on the speciation of the Candida albicans immunome in candidemia.

Data Brief 2016 Mar 11;6:257-61. Epub 2015 Dec 11.

Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS), Spain.

The characterization of pathogen-specific antigenic proteins at the protein species level is crucial in the development and molecular optimization of novel immunodiagnostics, vaccines or immunotherapeutics for infectious diseases. The major requirements to achieve this molecular level are to obtain 100% sequence coverage and identify all post-translational modifications of each antigenic protein species. In this article, we show nearly complete sequence information for five discrete antigenic species of Candida albicans Tdh3 (glyceraldehyde-3-phosphate dehydrogenase), which have been reported to be differentially recognized both among candidemia patients and between candidemia and control patients. Read More

View Article

Immunoproteomics-Based Analysis of the Immunocompetent Serological Response to Lomentospora prolificans.

J Proteome Res 2016 Feb 21;15(2):595-607. Epub 2016 Jan 21.

Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.

The filamentous fungus Lomentospora prolificans is an emerging pathogen causing severe infections mainly among the immunocompromised population. These diseases course with high mortality rates due to great virulence of the fungus, its inherent resistance to available antifungals, and absence of specific diagnostic tools. Despite being widespread in humanized environments, L. Read More

View Article
February 2016
10 Reads

Identification and analysis of divergent immune gene families within the Tasmanian devil genome.

BMC Genomics 2015 Nov 26;16:1017. Epub 2015 Nov 26.

Faculty of Veterinary Science, University of Sydney, Camperdown, NSW, Australia.

Background: The Tasmanian devil (Sarcophilus harrisii) is being threatened with extinction in the wild by a disease known as devil facial tumour disease (DFTD). In order to prevent the spread of this disease a thorough understanding of the Tasmanian devil immune system and its response to the disease is required. In 2011 and 2012 two genome sequencing projects of the Tasmania devil were released. Read More

View Article
November 2015
6 Reads

Novel Humoral Prognostic Markers in Small-Cell Lung Carcinoma: A Prospective Study.

PLoS One 2015 25;10(11):e0143558. Epub 2015 Nov 25.

Division of Clinical Neuroscience, University of Nottingham, Queen's Medical Centre, Nottingham, United Kingdom.

Purpose: Favourable small cell lung carcinoma (SCLC) survival outcomes have been reported in patients with paraneoplastic neurological disorders (PNDs) associated with neuronal antibodies (Neur-Abs), but the presence of a PND might have expedited diagnosis. Our aim was to establish whether neuronal antibodies, independent of clinical neurological features, correlate with SCLC survival.

Experimental Design: 262 consecutive SCLC patients were examined: of these, 24 with neurological disease were excluded from this study. Read More

View Article
June 2016
11 Reads

Seroprofiling at the Candida albicans protein species level unveils an accurate molecular discriminator for candidemia.

J Proteomics 2016 Feb 17;134:144-162. Epub 2015 Oct 17.

Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS), Spain.

Serum antibodies to specific Candida proteins have been reported as potential diagnostic biomarkers for candidemia. However, their diagnostic usefulness at the protein species level has hardly been examined. Using serological proteome analysis, we explored the IgG-antibody responses to Candida albicans protein species in candidemia and control patients. Read More

View Article
February 2016

Deciphering the human immunome.

Expert Rev Vaccines 2015 24;14(11):1421-5. Epub 2015 Aug 24.

b 2 International AIDS Vaccine Initiative, New York, NY, USA.

Technological advances in next-generation DNA sequencing offer great potential to probe the human immune system. On 3 April 2015, leading immunologists and bioinformatics scientists met to consider how best to harness these advances for decoding the human immunome. Read More

View Article
July 2016
4 Reads

Establishing the pig as a large animal model for vaccine development against human cancer.

Front Genet 2015 15;6:286. Epub 2015 Sep 15.

Department of Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark Copenhagen, Denmark.

Immunotherapy has increased overall survival of metastatic cancer patients, and cancer antigens are promising vaccine targets. To fulfill the promise, appropriate tailoring of the vaccine formulations to mount in vivo cytotoxic T cell (CTL) responses toward co-delivered cancer antigens is essential. Previous development of therapeutic cancer vaccines has largely been based on studies in mice, and the majority of these candidate vaccines failed to induce therapeutic responses in the subsequent human clinical trials. Read More

View Article
October 2015
3 Reads