J Vis Exp 2022 Jun 3(184). Epub 2022 Jun 3.
Women's Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center; The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University; Women's Health Integrated Research Center, Inova Women's Service Line, Inova Health System;
The tumor microenvironment (TME) represents a complex ecosystem comprised of dozens of distinct cell types, including tumor, stroma, and immune cell populations. To characterize proteome-level variation and tumor heterogeneity at scale, high-throughput methods are needed to selectively isolate discrete cellular populations in solid tumor malignancies. This protocol describes a high-throughput workflow, enabled by artificial intelligence (AI), that segments images of hematoxylin and eosin (H&E)-stained, thin tissue sections into pathology-confirmed regions of interest for selective harvest of histology-resolved cell populations using laser microdissection (LMD). Read More